A transfer factor detection apparatus and method

Abstract

The application discloses a kind of transfer factor detection equipment and method, it is related to biological detection technical field, including microplate reader, detection chamber, microporous plate, detection component, the inner wall of the detection chamber is symmetrically provided with chute, and the inner wall of chute is connected with tray with sliding, the bottom of the tray is provided with push positioning mechanism, to in push detection, the detection position of the microporous plate is fixed with adhesion.This kind of transfer factor detection equipment, in push detection process, can be rotated by driving tray to drive gear, using the cooperation of guide groove and shaft, four positioning blocks are synchronously moved inward, so that four positioning blocks can be used to adhere positioning four sidewalls of microporous plate when being pushed to detection position, guarantee the accuracy of microporous plate position in detection process, in turn guarantee the accuracy of subsequent detection result of the equipment.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, specifically to a device and method for detecting transfer factor. Background Technology

[0002] With the development of modern animal husbandry and the increasing scale and intensification of farming, disease has become a major problem plaguing the industry. The current common solution is immunization; however, due to various reasons, immunization sometimes fails, leading to outbreaks of atypical infectious diseases, which pose challenges to diagnosis and treatment. Transfer factor, released by immune-active T lymphocytes, is a factor that can transfer immune sensitization information. It can specifically transfer the immune function of a donor cell to a recipient animal, non-specifically enhancing the recipient animal's immune function. It is a novel immune stimulant widely used to enhance the immune function of poultry and prevent and treat viral diseases.

[0003] Since transfer factor is an important immunomodulatory factor in poultry, it is necessary to detect its level after injection to assess the integrity of the poultry's immune function and detect potential immune deficiencies in a timely manner. Current detection methods include enzyme-linked immunosorbent assay (ELISA), Western blotting, and mass spectrometry. Among them, ELISA is widely used due to its advantages of high sensitivity, high specificity, and ease of operation. The ELISA analyzer, also known as an ELISA reader or microplate detector, is a dedicated instrument for ELISA. It uses colorimetric analysis and, in conjunction with appropriate reagents, is used for the qualitative and quantitative analysis of transfer factor in biological samples.

[0004] Currently, when using an ELISA reader, the operator places the microplate containing the enzyme on a pull-out tray and then pushes the tray into the reader to detect the transfer factor in the sample. The operator positions the microplate during placement, but the lack of positioning components during tray insertion makes it impossible to guarantee the accuracy of the microplate's position within the reader. This can lead to the microplate tilting or shifting. Furthermore, due to its inherent instability during movement, the solution inside the microplate can easily overflow and mix with the solutions in other wells, causing contamination and affecting the accuracy of subsequent ELISA reader results. Summary of the Invention

[0005] The purpose of this invention is to provide a device and method for detecting transfer factors, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides a detection device for transfer factor, comprising an enzyme-linked immunosorbent assay (ELISA) reader, a detection chamber, a microplate, and a detection component. The inner wall of the detection chamber is symmetrically provided with grooves, and a tray is slidably connected to the inner wall of the groove.

[0007] The bottom of the tray is provided with a push positioning mechanism to fit and fix the detection position of the microporous plate during push detection;

[0008] The top of the tray is symmetrically provided with a push protection mechanism to block the upper surface of the microporous plate during push detection;

[0009] The inner wall of the detection chamber is symmetrically equipped with a push-pull cleaning mechanism to clean the sealing surface before and after sealing during the push-to-detection process.

[0010] Preferably, the pushing and positioning mechanism includes a main rod, the top of which is rotatably connected to the bottom of the tray. A large gear is fixedly connected to the bottom of the main rod, and a small gear meshes with one side of the large gear. A secondary rod is fixedly connected to the top of the small gear, and the secondary rod is rotatably connected to the bottom of the tray. A rack is fixedly connected to one side of the inner wall of the detection chamber corresponding to the small gear, and one side of the small gear meshes with the tooth groove of the rack.

[0011] Preferably, the top of the large gear is provided with guide grooves at equal intervals, and the inner wall of the guide groove is slidably connected to a shaft. The top of the shaft is fixedly connected to a positioning block. The top of the tray is provided with a limiting groove corresponding to the positioning block, and the outer walls of the two sides of the positioning block slide against the inner wall of the limiting groove. The inner wall of the limiting groove is symmetrically provided with support grooves, and the outer walls of the two sides of the positioning block are fixedly connected to the support grooves, and the outer walls of the support blocks slide against the inner wall of the support groove.

[0012] Preferably, the pushing protection mechanism includes a first fixing block, the bottom of which is fixedly connected to the top of the tray. A rotating rod is slidably connected inside the first fixing block, and a second fixing block is slidably connected to the outer wall of the other end of the rotating rod. The bottom of the second fixing block is fixedly connected to the top of the tray. A baffle is fixedly connected to the outer wall of the rotating rod. The tops of the two baffles are inclined. A nano-coating is sprayed on the side of the two baffles that are close to each other. A first spring is attached to the outer wall of the end of the rotating rod that is close to the second fixing block.

[0013] Preferably, one end of the first spring is fixedly connected to one side wall of the second fixing block, and the other end of the first spring is fixedly connected to a support ring. The outer wall of the end of the rotating rod near the second fixing block is provided with a rotating groove corresponding to the support ring, and the inner wall of the support ring is in contact with the inner wall of the rotating groove for rotation. The outer wall of the end of the rotating rod near the first fixing block is fixedly connected to a locking strip, and the side wall of the first fixing block away from the second fixing block is provided with a locking groove corresponding to the locking strip. The outer wall of the locking strip is in contact with the inner wall of the locking groove for sliding.

[0014] Preferably, torsion springs are provided at equal intervals on the outer wall of the rotating rod, and one end of the torsion springs is attached to the outer wall of the rotating rod. The other ends of the two torsion springs are respectively attached to the side wall of the first fixing block and the second fixing block that are close to each other. The side wall of the first fixing block that is away from the second fixing block is fixedly connected to the corresponding locking strip with a locking post. The side of the two baffles that are far apart is fixedly connected to the corresponding push-pull cleaning mechanism with a trigger top block.

[0015] Preferably, the push-pull cleaning mechanism includes a slide rod, with both ends of the slide rod fixedly connected to the inner wall of the detection chamber. A slider is slidably connected to the outer wall of the slide rod, and a mounting groove is provided on one side wall of the slider. An mounting block is slidably connected to the inner wall of the mounting groove, and a cleaning block is fixedly connected to the bottom of the mounting block. A cleaning cavity is provided through one side wall of the cleaning block corresponding to the baffle, and the inner wall of the cleaning cavity is slidably attached to the outer wall of the baffle. An electrostatic adsorption film is provided on the inner wall of the cleaning cavity.

[0016] Preferably, a second spring is fitted to the outer wall of the slide bar, one end of the second spring is fixedly connected to the inner wall of the detection chamber, and the other end of the second spring is fixedly connected to one side wall of the slide bar.

[0017] Preferably, a tray plate is fixedly installed on one side of the tray, and a handle is fixedly installed on one side of the tray plate.

[0018] This invention provides a method for detecting transfer factors, comprising the following steps:

[0019] Pushing and positioning: First, pull the tray out of the detection chamber. Then, carefully place the microplate in the center of the tray and manually press the two baffles to fit against the upper surface of the microplate. Then, push the tray back into the detection chamber. At this time, the four positioning blocks move inward synchronously during the pushing process so that when pushed to the detection position, the four positioning blocks can fit and position the four side walls of the microplate.

[0020] Push protection: When the baffle is pressed from the vertical position to the horizontal position, the first spring is used to make the locking strip slide into the slot to fix the two baffles and block the upper surface of the microporous plate during the pushing process. When the microporous plate moves to the detection position, the locking strip will disengage from the slot. At this time, the torsion spring is used to make the two baffles return to the vertical position to prevent the baffles from blocking the detection position.

[0021] Push-pull cleaning: During the pushing process, one side of the two trigger top blocks can contact and push one side of the cleaning block. When the two baffles remain vertical after resetting, the cleaning block returns to its original position under the rebound of the second spring and uses the electrostatic adsorption film in the cleaning chamber to adsorb and clean the dust on the surface of the baffle after sealing. When the tray is pulled out, the baffle can slide from the cleaning block to the outside, ensuring the cleanliness of the sealing surface before and after sealing.

[0022] Compared with the prior art, the beneficial effects of the present invention are:

[0023] 1. During the push-to-detection process, the large gear is rotated by pushing the tray. The guide groove and shaft work together to make the four positioning blocks move inward synchronously. When pushed to the detection position, the four positioning blocks can fit and position the four side walls of the microplate, ensuring the accuracy of the microplate position during the detection process, and thus ensuring the accuracy of the subsequent detection results of this equipment.

[0024] 2. During the push-to-detection process, after placing the microplate, the two baffles can be manually pressed to adhere to and fix them to the upper surface of the microplate. This seals the upper surface of the microplate during push-to-detection, preventing the solution inside the microplate from overflowing and mixing with other solutions, thus avoiding contamination. At the same time, when pushed to the detection position, the two baffles can automatically release their self-fixation, preventing the baffles from obstructing the detection position, thereby ensuring the accuracy of the subsequent detection results of this equipment.

[0025] 3. During the push-to-detection process, the trigger block can be used to charge and push the cleaning block, so that when the two baffles are reset to the vertical position, the cleaning chamber can be used to clean the sealing surface of the baffles. At the same time, when the tray is pulled out after detection to detect the next set of samples, the reset cleaning block can be used to clean the sealing surface of the baffles again, ensuring the cleanliness of the sealing surface before and after the baffles are sealed, preventing the solution from coming into contact with and mixing with the dust on the sealing surface when shaking, ensuring the purity of the solution during detection, and thus ensuring the accuracy of the subsequent detection results of this equipment. Attached Figure Description

[0026] Figure 1 This is a flowchart of the detection process of the present invention;

[0027] Figure 2 This is a schematic diagram of the overall structure of the present invention;

[0028] Figure 3 This is a schematic diagram of the tray in the extended state of the present invention;

[0029] Figure 4 This is a schematic diagram of the internal structure of the detection chamber of the present invention;

[0030] Figure 5 This is a schematic diagram of the push positioning mechanism of the present invention;

[0031] Figure 6 This is a schematic diagram of the structure of the first part of the push protection mechanism of the present invention;

[0032] Figure 7 This is a schematic diagram of the structure of the second part of the push protection mechanism of the present invention;

[0033] Figure 8 This is a schematic diagram of the push-pull cleaning mechanism of the present invention.

[0034] In the diagram: 1. Microplate reader; 2. Detection chamber; 3. Slide; 4. Tray; 8. Rack; 9. Microplate; 10. Detection component; 11. Support plate; 5. Pushing and positioning mechanism; 501. Main rod; 502. Large gear; 503. Small gear; 504. Secondary rod; 505. Guide groove; 506. Shaft; 507. Positioning block; 508. Limiting groove; 509. Support groove; 510. Support block; 6. Pushing and protective mechanism; 601. First fixing... 602. Fixed block; 603. Rotating rod; 604. Second fixed block; 605. Baffle; 606. First spring; 607. Support ring; 608. Rotating groove; 609. Locking strip; 610. Locking slot; 611. Torsion spring; 612. Locking post; 613. Trigger top block; 7. Push-pull cleaning mechanism; 701. Sliding rod; 702. Sliding block; 703. Mounting groove; 704. Mounting block; 705. Cleaning block; 706. Cleaning cavity; 707. Second spring. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Example 1, please refer to Figure 1-8 The present invention provides a detection device for transfer factor, including an enzyme-linked immunosorbent assay (ELISA) reader 1, a detection chamber 2, a microplate 9, and a detection component 10. The inner wall of the detection chamber 2 is symmetrically provided with grooves 3, and a tray 4 is slidably connected to the inner wall of the grooves 3.

[0037] The bottom of the tray 4 is provided with a push positioning mechanism 5. Further, the push positioning mechanism 5 includes a main rod 501, and the top of the main rod 501 is rotatably connected to the bottom of the tray 4. A large gear 502 is fixedly connected to the bottom of the main rod 501, and a small gear 503 is meshed on one side of the large gear 502. A secondary rod 504 is fixedly connected to the top of the small gear 503, and the secondary rod 504 is rotatably connected to the bottom of the tray 4. A rack 8 is fixedly connected to one side of the inner wall of the detection chamber 2 corresponding to the small gear 503, and one side of the small gear 503 meshes with the tooth groove of the rack 8.

[0038] Furthermore, after pushing the microplate 9 to the detection position, click the start detection button. The microplate reader 1 will use the detection component 10 to make each well enter the light beam for detection in sequence. The detection principle of this product is an existing technology product, so the specific detection principle will not be described in detail here.

[0039] In this embodiment, when preparing to place the microplate 9, the tray 4 is first pulled out of the detection chamber 2, then the microplate 9 is carefully placed in the center of the tray 4, and then the tray 4 is pushed back into the detection chamber 2. The top of the main rod 501 is rotatably connected to the bottom of the tray 4, and a large gear 502 is fixedly connected to the bottom of the main rod 501. A small gear 503 meshes with one side of the large gear 502. At the same time, a secondary rod 504 is fixedly connected to the top of the small gear 503, and the rotation of the secondary rod 504 is connected to the bottom of the tray 4. A rack 8 is fixedly connected to one side of the inner wall of the detection chamber 2 corresponding to the small gear 503. Thus, during the process of pushing the tray 4, the rack 8 can drive the small gear 503 to rotate, thereby synchronously driving the large gear 502 to rotate.

[0040] Next, guide grooves 505 are equidistantly opened through the top of the large gear 502, and a shaft 506 is slidably connected to the inner wall of the guide groove 505. A positioning block 507 is fixedly connected to the top of the shaft 506. At the same time, a limiting groove 508 is opened through the top of the tray 4 corresponding to the positioning block 507. The outer walls on both sides of the positioning block 507 slide against the inner wall of the limiting groove 508. Thus, during the rotation of the large gear 502, the positioning block 507 can be restricted by the limiting groove 508, allowing the shaft 506 to slide from the inner wall of the farthest end of the guide groove 505 to the inner wall of the nearest end. This allows the four positioning blocks 507 to move inward synchronously during the pushing process. When pushed to the detection position, the four positioning blocks 507 can be used to fit and position the four side walls of the microporous plate 9, ensuring the accuracy of the position of the microporous plate 9 during the detection process.

[0041] Next, support grooves 509 are symmetrically provided on the inner wall of the limiting groove 508, and support blocks 510 are fixedly connected to the outer walls of the positioning block 507 on both sides corresponding to the support grooves 509. The outer wall of the support block 510 slides against the inner wall of the support groove 509, so that the positioning block 507 can be supported by the cooperation of the support block 510 and the support groove 509, ensuring the stability of the positioning block 507 during movement.

[0042] As described above, during the push-to-detection process, the large gear 502 can be rotated by pushing the tray 4. With the cooperation of the guide groove 505 and the shaft 506, the four positioning blocks 507 move inward synchronously. When pushed to the detection position, the four positioning blocks 507 can fit and position the four side walls of the microporous plate 9, ensuring the accuracy of the position of the microporous plate 9 during the detection process, and thus ensuring the accuracy of the subsequent detection results of this equipment.

[0043] Example 2:

[0044] Based on the above embodiments, a push protection mechanism 6 is symmetrically arranged on the top of the tray 4. Further, the push protection mechanism 6 includes a first fixing block 601, and the bottom of the first fixing block 601 is fixedly connected to the top of the tray 4. A rotating rod 602 is slidably connected inside the first fixing block 601, and a second fixing block 603 is slidably connected to the outer wall of the other end of the rotating rod 602. The bottom of the second fixing block 603 is fixedly connected to the top of the tray 4. A baffle 604 is fixedly connected to the outer wall of the rotating rod 602. The tops of the two baffles 604 are inclined. A nano-coating is sprayed on the side of the two baffles 604 that are close to each other. A first spring 605 is attached to the outer wall of the end of the rotating rod 602 that is close to the second fixing block 603.

[0045] In this embodiment, during the push detection process, a rotating rod 602 is first slidably connected to the inside of the first fixing block 601, and a second fixing block 603 is slidably connected to the outer wall of the other end of the rotating rod 602. A baffle 604 is fixedly connected to the outer wall of the rotating rod 602, and the tops of the two baffles 604 are inclined, allowing the two baffles 604 to be manually pressed against the upper surface of the microporous plate 9 after placement. Next, a first spring 605 is attached to the outer wall of the rotating rod 602 near the second fixing block 603, and a support ring 606 is fixedly connected to the other end of the first spring 605. The inner wall of the support ring 606 is attached to the inner wall of the rotating groove 607 for rotation. Simultaneously, the rotating rod 602 rotates near the outer wall of the first fixing block 601. A retaining strip 608 is fixedly connected to the outer wall of the end, and a retaining groove 609 is opened on the side wall of the first fixing block 601 away from the second fixing block 603 corresponding to the retaining strip 608. The outer wall of the retaining strip 608 slides against the inner wall of the retaining groove 609. When the baffle 604 is pressed from the vertical state to the horizontal state, it can drive the retaining strip 608 to rotate to the position of the retaining groove 609, so that the retaining strip 608 and the retaining groove 609 are aligned. At this time, the first spring 605 drives the rotating rod 602 to slide, so that the retaining strip 608 slides into the retaining groove 609, automatically fixing the two baffles 604, so as to seal the upper surface of the microporous plate 9 during the pushing process, and prevent the solution loaded inside the microporous plate 9 from overflowing and mixing with other solutions during the movement, thus preventing contamination.

[0046] A nano-coating is sprayed on one side of the two baffles 604 that are close to each other. This allows the solution to be prevented from sticking to the sealing surface when it sloshes during the sealing process, thus avoiding a decrease in the solution content in the micropores and ensuring the accuracy of the subsequent test results of this equipment.

[0047] Then, during the pushing process, when the microplate 9 approaches the detection position, one end of the rotating rod 602 abuts against the inner wall of the detection chamber 2. Continuing to push the microplate 9 to the detection position, the rotating rod 602, constrained by the inner wall of the detection chamber 2, causes the retaining strip 608 to disengage from the retaining slot 609. At this point, torsion springs 610 are evenly spaced along the outer wall of the rotating rod 602, with one end of each torsion spring 610 resting on the outer wall of the rotating rod 602, and the other ends of the two torsion springs 610 respectively resting on the... The side wall of the first fixed block 601 is close to the side wall of the second fixed block 603, so that the torsion spring 610 can drive the rotating rod 602 to reverse and drive the two baffles 604 to rotate in the opposite direction. The side wall of the first fixed block 601 away from the second fixed block 603 is fixedly connected to the corresponding locking strip 608 with the locking post 611, so as to limit the reverse rotation position of the baffles 604, so that the two baffles 604 can accurately maintain the vertical state after resetting, and avoid the baffles 604 from blocking the detection position.

[0048] As described above, during the push-to-detection process, after placing the microplate 9, the two baffles 604 can be manually pressed to adhere and fix to the upper surface of the microplate 9, thereby sealing the upper surface of the microplate 9 during the push-to-detection process. This prevents the solution loaded inside the microplate 9 from overflowing and mixing with other solutions, thus avoiding contamination. At the same time, when pushed to the detection position, the two baffles 604 can automatically release their self-fixation, preventing the baffles 604 from obstructing the detection position, thereby ensuring the accuracy of the subsequent detection results of this device.

[0049] Example 3:

[0050] Based on the above embodiments, the inner wall of the detection chamber 2 is symmetrically provided with a push-pull cleaning mechanism 7. Further, the push-pull cleaning mechanism 7 includes a slide rod 701, and the two ends of the slide rod 701 are fixedly connected to the inner wall of the detection chamber 2. The outer wall of the slide rod 701 is slidably connected to a slider 702, and a mounting groove 703 is opened on one side wall of the slider 702. The inner wall of the mounting groove 703 is slidably connected to a mounting block 704, and a cleaning block 705 is fixedly connected to the bottom of the mounting block 704. A cleaning cavity 706 is opened through one side wall of the cleaning block 705 corresponding to the baffle 604. The inner wall of the cleaning cavity 706 is slidably attached to the outer wall of the baffle 604. An electrostatic adsorption film is provided on the inner wall of the cleaning cavity 706.

[0051] In this embodiment, a slider 702 is slidably connected to the outer wall of the slide rod 701, and a mounting groove 703 is provided on one side wall of the slider 702. A mounting block 704 is slidably connected to the inner wall of the mounting groove 703, and a cleaning block 705 is fixedly connected to the bottom of the mounting block 704. A second spring 707 is fitted to the outer wall of the slide rod 701. Therefore, when the tray 4 is not pushed in, both sliders 702 are located at the entrance of the detection chamber 2 under the elastic force of the second spring 707. The side wall of the mounting block 704 is also slidably connected to the inner wall of the mounting block 704 under the elastic force of the second spring 707. The cleaning block 705 is pressed against the inner wall of the entrance of the detection chamber 2 to install and fix it, preventing the cleaning block 705 from separating from the slider 702. Then, when the tray 4 is pushed after the two baffles 604 are pressed to a horizontal state, the trigger top block 612 is fixedly connected to the push-pull cleaning mechanism 7 through the side of the two baffles 604 that is far apart. This allows one side of the two trigger top blocks 612 to contact and push one side of the cleaning block 705, causing the cleaning block 705 to move deeper into the inner wall of the detection chamber 2 and compress the second spring 707.

[0052] When the two baffles 604 remain vertical after being reset, a cleaning cavity 706 is formed through one side wall of the cleaning block 705 corresponding to the baffle 604. The inner wall of the cleaning cavity 706 slides against the outer wall of the baffle 604, and an electrostatic adsorption film is provided on the inner wall of the cleaning cavity 706. This allows the cleaning block 705 to slide from both sides of the baffle 604 under the rebound of the second spring 707. The electrostatic adsorption film in the cleaning cavity 706 is used to adsorb and clean the dust on the surface of the baffle 604 after sealing. Similarly, when the tray 4 is pulled out after the test is completed to test the next set of samples, the baffle 604, which is in a vertical state, can slide from the cleaning block 705 to the outside. The reset cleaning block 705 can be used to clean the sealing surface of the baffle 604 again, ensuring the cleanliness of the sealing surface before and after sealing the baffle 604. This prevents the solution from coming into contact with and mixing with the dust on the sealing surface when it shakes during the sealing process, thereby ensuring the purity of the solution during the test.

[0053] Meanwhile, when cleaning the cleaning chamber 706, since the cleaning block 705 is located at the outlet of the detection chamber 2 under normal circumstances, it is convenient to put your hand in. You can manually push the cleaning block 705 a certain distance into the depth of the detection chamber 2, and then press the slider 702 with your other hand. At this time, the cleaning block 705 can be removed to facilitate cleaning the cleaning chamber 706.

[0054] As described above, during the push-to-detection process, the trigger top block 612 can be used to push the cleaning block 705 with stored force. When the two baffles 604 are reset to the vertical position, the cleaning chamber 706 can be used to clean the sealing surface of the baffles 604. At the same time, when the tray 4 is pulled out after the detection is completed to detect the next set of samples, the reset cleaning block 705 can be used to clean the sealing surface of the baffles 604 again. This ensures the cleanliness of the sealing surface before and after the baffles 604 are sealed, and prevents the solution from coming into contact with and mixing with the dust on the sealing surface when it is shaken. This ensures the purity of the solution during detection, and thus ensures the accuracy of the subsequent detection results of this equipment.

[0055] The detection method of this transfer factor detection device is as follows: First, pull the tray 4 out of the detection chamber 2. Then, carefully place the microplate 9 in the center of the tray 4. Manually press the two baffles 604 to fit against the upper surface of the microplate 9. Then, push the tray 4 back into the detection chamber 2. During the process of pushing the tray 4, the rack 8 can drive the pinion 503 to rotate, which in turn drives the large gear 502 to rotate synchronously. During the rotation of the large gear 502, the positioning block 507 can be restricted by the limiting groove 508, so that the shaft 506 slides from the inner wall of the farthest end of the guide groove 505 to the inner wall of the nearest end. This allows the four positioning blocks 507 to move inward synchronously during the pushing process, so that when pushed to the detection position, the four positioning blocks 507 can fit and position the four side walls of the microplate 9.

[0056] Next, when the baffle 604 is pressed from the vertical state to the horizontal state, it can drive the locking strip 608 to rotate to the position of the locking groove 609, so that the locking strip 608 and the locking groove 609 are aligned. At this time, the first spring 605 uses its elastic force to drive the rotating rod 602 to slide, so that the locking strip 608 slides into the locking groove 609, automatically fixing the two baffles 604, so as to seal the upper surface of the microporous plate 9 during the pushing process;

[0057] When the microplate 9 approaches the detection position, one end of the rotating rod 602 abuts against the inner wall of the detection chamber 2. Continuing to push the rod causes the microplate 9 to move to the detection position. At this point, the rotating rod 602, constrained by the inner wall of the detection chamber 2, causes the retaining strip 608 to disengage from the retaining groove 609. Then, the torsion spring 610 drives the rotating rod 602 to reverse, causing the two baffles 604 to rotate in the opposite direction. The retaining pin 611 restricts the reverse rotation of the baffles 604, ensuring that the two baffles 604 accurately maintain a vertical position after resetting, preventing the baffles 604 from obstructing the detection position.

[0058] Next, when the tray 4 is pushed after the two baffles 604 are pressed to a horizontal position, a trigger block 612 is fixedly connected to the push-pull cleaning mechanism 7 via the opposite side of the two baffles 604. This allows one side of the two trigger blocks 612 to contact and push one side of the cleaning block 705, causing the cleaning block 705 to move deeper into the inner wall of the detection chamber 2 and compress the second spring 707. When the two baffles 604 remain vertical after resetting, the cleaning block 705... The spring 707 slides from both sides of the baffle 604 under its rebound, and the electrostatic adsorption film in the cleaning chamber 706 adsorbs and cleans the dust on the surface of the baffle 604 after sealing. Similarly, when the tray 4 is pulled out after the test is completed to test the next set of samples, the baffle 604, which is in a vertical state at this time, can slide into the outside through the cleaning block 705. The reset cleaning block 705 can be used to clean the sealing surface of the baffle 604 again, ensuring the cleanliness of the sealing surface before and after sealing the baffle 604.

[0059] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for detecting a transfer factor, characterized by, Including enzyme mark appearance (1), detection chamber (2), microporous plate (9), detection component (10), the inner wall of detection chamber (2) is symmetrically provided with sliding groove (3), and the inner wall of sliding groove (3) is connected with tray (4) by sliding. The bottom of the tray (4) is provided with a push positioning mechanism (5) to fix the detection position of the microporous plate (9) during detection. The top of the tray (4) is symmetrically provided with a pushing protection mechanism (6) near both sides of the chute (3), the pushing protection mechanism (6) comprises a first fixed block (601), and the bottom of the first fixed block (601) is fixedly connected to the top of the tray (4); a rotating rod (602) is slidably connected to the inside of the first fixed block (601); the other end of the rotating rod (602) is slidably connected with a second fixed block (603), and the bottom of the second fixed block (603) is fixedly connected to the top of the tray (4); a baffle (604) is fixedly connected to the outer wall of the rotating rod (602); the top of the two baffles (604) is beveled; the side of the two baffles (604) close to each other is sprayed with a nano coating; a first spring (605) is attached to the outer wall of one end of the rotating rod (602) close to the second fixed block (603); one end of the first spring (605) is fixedly connected to one side wall of the second fixed block (603); the other end of the first spring (605) is fixedly connected with a supporting ring (606); a rotating groove (607) is formed in the outer wall of one end of the rotating rod (602) close to the second fixed block (603) and corresponding to the supporting ring (606); the inner wall of the supporting ring (606) is rotatably attached to the inner wall of the rotating groove (607); a clamping strip (608) is fixedly connected to the outer wall of one end of the rotating rod (602) close to the first fixed block (601); a clamping groove (609) is formed in the side wall of the first fixed block (601) away from the second fixed block (603) and corresponding to the clamping strip (608); the outer wall of the clamping strip (608) is slidably attached to the inner wall of the clamping groove (609); when the baffle (604) is pressed from a vertical state to a horizontal state, the clamping strip (608) is rotated to the position of the clamping groove (609), so that the clamping strip (608) is aligned with the clamping groove (609); at this time, the rotating rod (602) is slid by the elastic force of the first spring (605), so that the clamping strip (608) slides into the clamping groove (609), and the two baffles (604) are automatically fixed, so as to block the upper surface of the microplate (9) during pushing; torsional springs (610) are equidistantly arranged on the outer wall of the rotating rod (602), one end of each torsional spring (610) is overlapped with the outer wall of the rotating rod (602), and the other end of each torsional spring (610) is overlapped with the side wall of the first fixed block (601) and the second fixed block (603) close to each other; a clamping column (611) is fixedly connected to the side wall of the first fixed block (601) away from the second fixed block (603) and corresponding to the clamping strip (608); trigger top blocks (612) are fixedly connected to the side of the two baffles (604) away from each other and corresponding to the pushing and pulling cleaning mechanism (7); when the microplate (9) approaches the detection position, one end of the rotating rod (602) abuts against the inner wall of the detection chamber (2); at this time, the microplate (9) is moved to the detection position by continuing to push,At this time, the rotating rod (602) is limited by the inner wall of the detection chamber (2) to drive the clamping strip (608) to separate from the clamping groove (609), the torsional spring (610) drives the rotating rod (602) to reverse, drives the two baffles (604) to reverse, the side wall of the first fixed block (601) away from the second fixed block (603) is fixedly connected with the clamping column (611) corresponding to the clamping strip (608), the reverse rotating position of the baffle (604) can be limited, so that the two baffles (604) can accurately keep the vertical state after resetting. The inner wall of the detection chamber (2) is symmetrically provided with a push-pull cleaning mechanism (7) to clean the sealing surface of the baffle (604) before and after sealing during detection.

2. The detection apparatus of a transfer factor according to claim 1, wherein The push positioning mechanism (5) includes a main rod (501), and the top of the main rod (501) is rotatably connected to the bottom of the tray (4). The bottom of the main rod (501) is fixedly connected with a large gear (502), and one side of the large gear (502) is engaged with a small gear (503). The top of the small gear (503) is fixedly connected with a secondary rod (504), and the secondary rod (504) is rotatably connected to the bottom of the tray (4). One side of the inner wall of the detection chamber (2) is fixedly connected with a rack (8) corresponding to the small gear (503), and one side of the small gear (503) is engaged with the tooth groove of the rack (8).

3. The device for detecting a transfer factor according to claim 2, wherein The top of the large gear (502) is provided with a guide groove (505) at equal distances, and the inner wall of the guide groove (505) is slidably connected with a shaft rod (506). The top of the shaft rod (506) is fixedly connected with a positioning block (507). The top of the tray (4) is provided with a limiting groove (508) corresponding to the positioning block (507), and the outer walls of the two sides of the positioning block (507) slide with the inner walls of the limiting grooves (508). The inner walls of the limiting grooves (508) are symmetrically provided with support grooves (509). The outer walls of the two sides of the positioning block (507) are fixedly connected with support blocks (510) corresponding to the support grooves (509), and the outer walls of the support blocks (510) slide with the inner walls of the support grooves (509).

4. The device for detecting a transfer factor according to claim 3, wherein The push-pull cleaning mechanism (7) includes a sliding rod (701), and the two ends of the sliding rod (701) are fixedly connected to the inner wall of the detection chamber (2). The outer wall of the sliding rod (701) is slidably connected with a sliding block (702), and one side wall of the sliding block (702) is provided with a mounting groove (703). The inner wall of the mounting groove (703) is slidably connected with a mounting block (704), and the bottom of the mounting block (704) is fixedly connected with a cleaning block (705). One side wall of the cleaning block (705) is provided with a cleaning cavity (706) corresponding to the baffle (604), and the inner wall of the cleaning cavity (706) slides with the outer wall of the baffle (604). The inner wall of the cleaning cavity (706) is provided with an electrostatic adsorption film.

5. The device for detecting a transfer factor according to claim 4, wherein The outer wall of the sliding rod (701) is provided with a second spring (707), one end of the second spring (707) is fixedly connected to the inner wall of the detection chamber (2), and the other end of the second spring (707) is fixedly connected to one side wall of the sliding block (702).

6. The detection apparatus of a transfer factor according to claim 1, wherein One side of the tray (4) is fixedly provided with a supporting plate (11), and one side surface of the supporting plate (11) is fixedly provided with a handle.

7. A detection method of a detection apparatus for a transfer factor according to claim 5, characterized by, The method comprises the following steps: S1: positioning by pushing, the tray (4) is pulled out from the detection chamber (2), the microplate (9) is placed at the center position of the tray (4), two baffle plates (604) are manually pressed to be attached to the upper surface of the microplate (9), the tray (4) is pushed into the detection chamber (2) again, at this time, four positioning blocks (507) are synchronously moved inward during the pushing process, so that the four sidewalls of the microplate (9) are positioned by the four positioning blocks (507) when the tray (4) is pushed to the detection position; S2: protection by pushing, when the baffle plate (604) is pressed from the vertical state to the horizontal state, the clamping strip (608) is slid into the clamping groove (609) by the first spring (605), so that the two baffle plates (604) are fixed, and the upper surface of the microplate (9) is blocked during the pushing process, when the microplate (9) moves to the detection position, the clamping strip (608) is separated from the clamping groove (609), the two baffle plates (604) are reset to the vertical state by the torsional spring (610), so that the baffle plate (604) is prevented from shielding the detection position; S3: cleaning by pushing and pulling, one side surface of the two trigger top blocks (612) is in contact with one side of the cleaning block (705) during the pushing process, when the two baffle plates (604) are reset to the vertical state, the cleaning block (705) is reset under the rebound of the second spring (707) and the dust on the surface of the baffle plate (604) is adsorbed and cleaned by the electrostatic adsorption film in the cleaning cavity (706), and when the tray (4) is pulled out, the baffle plate (604) slides from the cleaning block (705) to the outside, so that the cleanliness of the surface of the baffle plate (604) before and after blocking is ensured.

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