A traditional Chinese medicine composition for treating gastric cancer, a preparation method and application thereof

Abstract

The application discloses a traditional Chinese medicine composition for treating gastric cancer and a preparation method and application thereof, and relates to the following raw medicinal materials in parts by weight: Baizhi 3-9 parts, Huanglian 3-9 parts, Renshen 3-9 parts, Shijiancha 15-30 parts, Yuxizuo 10-15 parts, Huzhang 9-15 parts and Wuyao 3-9 parts. The traditional Chinese medicine composition is developed on the basis of the pathogenesis of gastric cancer, research on the theory of Sanjiao system and laboratory research, has the functions of regulating qi and stomach, resolving stasis and relieving pain, removing blood stasis and detoxification, and dredging Sanjiao, and has good effects on the treatment of gastric cancer. The general therapeutic principle is to strengthen the body resistance and eliminate pathogenic factors, the treatment method is to regulate qi and stomach, resolve stasis and relieve pain, and remove blood stasis and detoxification. Experiment proves that the traditional Chinese medicine composition has good anti-gastric cancer effect and small toxicity, is a great innovation for treating gastric cancer, and has remarkable clinical significance.

Description

Technical Field

[0001] This invention relates to a traditional Chinese medicine formula for treating gastric cancer, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. Background Technology

[0002] Stomach cancer is a malignant tumor originating from the stomach wall. It is the fifth most common cancer worldwide and the third leading cause of cancer-related deaths. According to Globocan 2022 data, there were 691,000 new cases of stomach cancer globally, with Asia having the highest incidence rate, accounting for 71.4% of global cases. In China, there are approximately 359,000 new cases of stomach cancer and 260,000 deaths annually, ranking fifth in incidence and third in mortality among malignant tumors, respectively. Stomach cancer exhibits significant geographical distribution differences. Incidence rates are higher in East Asian countries, with China being a high-incidence area, with both incidence and mortality rates significantly exceeding the world average. This may be related to diet, Helicobacter pylori infection, and genetic factors. Treatment for stomach cancer focuses on surgery, chemotherapy, targeted therapy, and immunotherapy. With the continuous development of molecular biology, genetics, and precision medicine, stomach cancer treatment is becoming increasingly diversified, emphasizing individualized and multidisciplinary comprehensive treatment.

[0003] Traditional Chinese medicine (TCM) is gaining increasing importance in the comprehensive treatment of gastric cancer, particularly in its role as an adjuvant to chemotherapy, symptom relief, quality of life enhancement, and recurrence reduction. In recent years, with the deepening of basic research on TCM and the advancement of modern medical technology, the role of TCM in gastric cancer treatment has received clearer scientific evidence. Therefore, strengthening the research and development of novel TCM formulas for gastric cancer and further improving treatment efficacy is of great significance for enhancing clinical prevention and treatment capabilities and reducing the mortality rate of gastric cancer patients. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a traditional Chinese medicine formula, preparation method and application for treating gastric cancer, so as to play a role in the clinical diagnosis, treatment and prevention of gastric cancer, reduce the mortality rate of gastric cancer patients, prolong survival time and improve the quality of life of patients.

[0005] To achieve the above objectives, one aspect of the technical solution of the present invention is to provide a traditional Chinese medicine formula for treating gastric cancer, comprising the following raw materials in parts by weight: 3-9 parts Angelica dahurica, 3-9 parts Coptis chinensis, 3-9 parts Panax ginseng, 15-30 parts Hedyotis diffusa, 10-15 parts Ziziphus jujuba, 9-15 parts Polygonum cuspidatum, and 3-9 parts Lindera strychnifolia.

[0006] Preferably, the traditional Chinese medicine formula for treating gastric cancer includes the following raw materials in parts by weight: 3 parts Angelica dahurica, 3 parts Coptis chinensis, 3 parts ginseng, 15 parts Hedyotis diffusa, 10 parts Citrus aurantium, 9 parts Polygonum cuspidatum, and 3 parts Lindera strychnifolia.

[0007] On the other hand, the technical solution of the present invention provides a method for preparing the traditional Chinese medicine formula for treating gastric cancer, wherein each raw material is extracted with ethanol, then dried, and then the dried extracts are mixed to obtain the final product. Specifically, the method includes the following steps:

[0008] (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder.

[0009] (2) Add ethanol solution to each raw material, soak at room temperature in the dark, then add to a reflux flask for extraction, boil, filter, take the filtrate, recover the residue, repeat the above extraction steps, and take the filtrate.

[0010] (3) Mix the two filtrates of each raw material separately, put them on a rotary evaporator to remove ethanol, then evaporate the water in a water bath, freeze them in a refrigerator, freeze-dry them, and finally pulverize them into powder.

[0011] (4) Mix the powdered raw materials together to obtain the final product.

[0012] Furthermore, in step (2), the volume concentration of the ethanol solution is 70-75%, and the amount used is 8-10 times the volume of the raw drug.

[0013] Furthermore, the soaking time in step (2) is 24-48 hours.

[0014] Furthermore, the reflux temperature in step (2) is 90-100℃.

[0015] Furthermore, the evaporation temperature of the rotary evaporator in step (3) is 42-50℃.

[0016] Furthermore, the water bath temperature in step (3) is 70-80℃.

[0017] On the other hand, the technical solution of the present invention is to provide the application of the aforementioned traditional Chinese medicine formula in the preparation of drugs for treating gastric cancer.

[0018] Pharmacological effects of each active pharmaceutical ingredient in this invention:

[0019] Angelica dahurica, pungent and warm in nature; enters the stomach, large intestine, and lung meridians. Main functions: relieves exterior syndromes and dispels cold, dispels wind and relieves pain, clears nasal passages, dries dampness and stops leukorrhea, reduces swelling and drains pus.

[0020] Coptis chinensis, bitter and cold in nature; enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians. Main functions: clears heat and dries dampness, drains fire and detoxifies.

[0021] Ginseng is sweet, slightly bitter, and slightly warm in nature; it enters the spleen, lung, heart, and kidney meridians. Its main functions include: greatly replenishing vital energy, restoring pulse and consolidating the body, tonifying the spleen and lungs, generating fluids and nourishing blood, and calming the mind and improving intelligence.

[0022] Salvia chinensis Benth., pungent, bitter, slightly cold in nature; attributive to the liver and spleen meridians. Main effects: promoting blood circulation to remove stasis, clearing heat and promoting diuresis, dissipating binds and detumescence.

[0023] Fructus Akebiae, bitter, cold in nature; attributive to the liver, gallbladder, stomach and bladder meridians. Main effects: soothing the liver and regulating qi, activating blood circulation to stop pain, dissipating binds, inducing diuresis.

[0024] Polygonum cuspidatum Sieb. et Zucc., slightly bitter, slightly cold in nature; attributive to the liver, gallbladder and lung meridians. Main effects: promoting diuresis for treating jaundice, clearing heat and detoxifying, removing stasis and alleviating pain, relieving cough and resolving phlegm.

[0025] Lindera aggregata (Sims) Kosterm., pungent, warm in nature; attributive to the lung, spleen, kidney and bladder meridians. Main effects: promoting qi circulation to relieve pain, warming the kidney and dispelling cold.

[0026] Traditional Chinese medicine believes that gastric cancer belongs to the category of cancer diseases, which are mostly caused by factors such as improper diet, emotional disorders, weakness of the spleen and stomach, internal binding of static blood, stagnation of phlegm-dampness, invasion of pathogenic cold into the stomach, damp-heat of the spleen and stomach, etc. The basic pathogenesis is the stagnation of qi, phlegm-dampness and static blood in the stomach, resulting in disharmony of the stomach qi. The vital qi is deficient internally, and pathogenic toxins invade, and the disease occurs over time.

[0027] The inventors believe that examining stomach cancer from the perspective of the Triple Burner reveals a close correlation between its occurrence and pathological mechanisms such as obstruction of the Middle Burner, stagnation of Qi and blood, and internal accumulation of toxins. The Triple Burner governs all Qi and regulates water metabolism. When the Triple Burner is free of primordial Qi, stagnation leads to dampness, which in turn generates heat, affecting blood circulation and easily causing Qi stagnation and blood stasis. Qi stagnation leads to blood stasis, which in turn forms accumulations, which can transform into heat, potentially developing into stomach cancer. The Middle Burner, including the spleen, stomach, liver, and gallbladder, is the source of food digestion and absorption, as well as the production of Qi and blood. Stomach cancer, a malignant tumor of the digestive system, is closely related to functional abnormalities of the Middle Burner. When the Middle Burner is obstructed, spleen and stomach functions are impaired, Qi and blood production is insufficient, and the ascending and descending of Qi becomes abnormal, potentially leading to the accumulation of pathological products such as phlegm, dampness, and blood stasis, ultimately forming stomach cancer. Stomach cancer patients often have toxins such as damp heat, phlegm, and blood stasis in their bodies. These toxins accumulate in the Triple Burner, causing dysfunction of the internal organs, deficiency of vital Qi, and ultimately inducing stomach cancer. Therefore, in the treatment of stomach cancer, attention should be paid to comprehensive treatment that includes clearing the triple burner, regulating qi and blood, detoxifying and dispersing nodules, and overall conditioning. Therefore, this formula uses *Scutellaria baicalensis* and *Ziziphus jujuba* as the chief herbs. *Scutellaria baicalensis* invigorates blood circulation, removes blood stasis, clears heat and dampness, disperses nodules and reduces swelling. *Ziziphus jujuba* soothes the liver, regulates qi, detoxifies and relieves pain. Together, they regulate qi stagnation, clear heat stagnation, and prevent the Shao Yang fire from acting recklessly. Together, they unblock the qi mechanism and water passages of the three jiaos, ensuring the smooth flow of qi and blood and normal water metabolism. *Polygonum cuspidatum*, *Coptis chinensis*, and *Lindera strychnifolia* are the assistant herbs. *Polygonum cuspidatum* assists *Scutellaria baicalensis* in enhancing its blood-activating and stasis-removing effects, while also clearing heat, detoxifying, and dispersing blood stasis to relieve pain. It enters the liver and gallbladder meridians and regulates the three jiaos. *Coptis chinensis* assists *Scutellaria baicalensis* in enhancing its heat-clearing effects, while also drying dampness and purging fire to detoxify. *Lindera strychnifolia* assists *Ziziphus jujuba* in enhancing its qi-regulating, pain-relieving, and detoxifying effects, while also soothing the liver, regulating qi, dispersing nodules, and promoting urination. *Angelica dahurica* and ginseng expel pathogens, opening the way for their elimination, replenishing vital energy, tonifying the spleen and lungs, and enhancing the body's resistance and disease-fighting ability. When used together, these two herbs can both expel pathogens and strengthen the body's resistance, achieving a better therapeutic effect. The two herbs work together as adjuvants, complementing the combined effects of the principal and assistant herbs. Together, they regulate qi and harmonize the stomach, disperse stagnation and relieve pain, resolve blood stasis and detoxify, and unblock the three jiaos (upper, middle, and lower burners).

[0028] Beneficial effects

[0029] This invention's traditional Chinese medicine formula is based on the pathogenesis of gastric cancer and was developed through theoretical research and laboratory studies of the Triple Energizer system. It possesses the functions of regulating Qi and harmonizing the stomach, dispersing nodules and relieving pain, resolving blood stasis and detoxifying, and unblocking the Triple Energizer. It has shown good efficacy in the treatment of gastric cancer. The overall treatment principle is to strengthen the body's resistance and eliminate pathogenic factors, with the treatment methods being to regulate Qi and harmonize the stomach, disperse nodules and relieve pain, and resolve blood stasis and detoxify. Experiments have demonstrated that this invention's traditional Chinese medicine formula has good anti-gastric cancer effects with low toxicity, representing a significant innovation in the treatment of gastric cancer. Attached Figure Description

[0030] Figure 1 The effect of traditional Chinese medicine formulations on the epigenetic state of tumor cells.

[0031] Figure 2 The growth inhibition rate of the traditional Chinese medicine formula on tumor cells.

[0032] Figure 3 The effect of traditional Chinese medicine formulations on the migration ability of tumor cells is shown in the figure. In the figure, ns indicates no statistical significance. Detailed Implementation

[0033] The specific embodiments of the present invention will be further described in detail below with reference to examples.

[0034] Example 1

[0035] A traditional Chinese medicine formula for treating gastric cancer includes the following raw materials in parts by weight: Angelica dahurica 3 parts, Coptis chinensis 3 parts, ginseng 3 parts, Hedyotis diffusa 15 parts, Citrus aurantium 10 parts, Polygonum cuspidatum 9 parts, and Lindera strychnifolia 3 parts.

[0036] Its preparation method is as follows:

[0037] (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder.

[0038] (2) Soak the above raw materials in 8 times their volume of 70% (v / v) ethanol solution; at room temperature, cover with plastic wrap and aluminum foil to protect from light, and stir and shake with a glass rod twice from time to time for a total of 24 hours; then add the soaked raw materials to a reflux flask for extraction, boil at 90℃ for 1 hour, filter, take the filtrate, recover the residue, repeat the above steps again for reflux extraction, boiling, filtering, take the filtrate, filter with three layers of filter paper, and use an external air pump to speed up the filtration.

[0039] (3) Mix the two filtrates of each raw material, put them on a 45°C rotary evaporator to remove the ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 78°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and pulverize them into powder.

[0040] (4) Mix all the raw drug powders together and store at -20℃ to obtain the final product.

[0041] Example 2

[0042] A traditional Chinese medicine formula for treating gastric cancer includes the following raw materials in parts by weight: 6 parts Angelica dahurica, 6 parts Coptis chinensis, 3 parts ginseng, 30 parts Hedyotis diffusa, 15 parts Citrus aurantium, 12 parts Polygonum cuspidatum, and 9 parts Lindera strychnifolia.

[0043] Its preparation method is as follows:

[0044] (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder.

[0045] (2) Soak the above raw materials in 8 times their volume of 75% (v / v) ethanol solution; at room temperature, cover with plastic wrap and aluminum foil to protect from light, and stir and shake with a glass rod twice from time to time for a total of 24 hours; then add the soaked raw materials to a reflux flask for extraction, boil at 90℃ for 1 hour, filter, take the filtrate, recover the residue, repeat the above steps again for reflux extraction, boiling, filtering, take the filtrate, filter with three layers of filter paper, and use an external vacuum pump to speed up the filtration.

[0046] (3) Mix the two filtrates of each raw material, put them on a 42°C rotary evaporator to remove the ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 80°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and pulverize them into powder.

[0047] (4) Mix all the raw drug powders together and store at -20℃ to obtain the final product.

[0048] Example 3

[0049] A traditional Chinese medicine formula for treating gastric cancer includes the following raw materials in parts by weight: Angelica dahurica 7 parts, Coptis chinensis 7 parts, ginseng 7 parts, Hedyotis diffusa 20 parts, Citrus aurantium 12 parts, Polygonum cuspidatum 10 parts, and Lindera strychnifolia 6 parts.

[0050] Its preparation method is as follows:

[0051] (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder.

[0052] (2) Soak the above raw materials in 9 times their volume of 70% (v / v) ethanol solution; at room temperature, cover with plastic wrap and aluminum foil to protect from light, and stir and shake with a glass rod twice from time to time for a total of 36 hours; then add the soaked raw materials to a reflux flask for extraction, boil at 95℃ for 1 hour, filter, take the filtrate, recover the residue, repeat the above steps again for reflux extraction, boiling, filtering, take the filtrate, filter with three layers of filter paper, and use an external vacuum pump to speed up the filtration.

[0053] (3) Mix the two filtrates of each raw material, put them on a 50°C rotary evaporator to remove the ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 70°C, freeze them in a -80°C freezer, and finally dry them into a solid state using a freezer and pulverize them into powder.

[0054] (4) Mix all the raw drug powders together and store at -20℃ to obtain the final product.

[0055] Example 4

[0056] A traditional Chinese medicine formula for treating gastric cancer includes the following raw materials in parts by weight: Angelica dahurica 9 parts, Coptis chinensis 9 parts, ginseng 9 parts, Hedyotis diffusa 30 parts, Citrus aurantium 15 parts, Polygonum cuspidatum 15 parts, and Lindera strychnifolia 9 parts.

[0057] Its preparation method is as follows:

[0058] (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder.

[0059] (2) Soak the above raw materials in 10 times their volume of 75% (v / v) ethanol solution; at room temperature, cover with plastic wrap and aluminum foil to protect from light, and stir and shake with a glass rod twice from time to time for a total of 48 hours; then add the soaked raw materials to a reflux flask for extraction, boil at 97℃ for 1 hour, filter, take the filtrate, recover the residue, repeat the above steps again for reflux extraction, boil, filter, take the filtrate, filter with three layers of filter paper, and use an external vacuum pump to speed up the filtration.

[0060] (3) Mix the two filtrates of each raw material, put them on a 45°C rotary evaporator to remove the ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 75°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and pulverize them into powder.

[0061] (4) Mix all the raw drug powders together and store at -20℃ to obtain the final product.

[0062] Experiments on the inhibition of tumor growth by traditional Chinese medicine formulations for gastric cancer:

[0063] 1. Materials

[0064] 1.1 Cell lines

[0065] Human gastric cancer cell line AGS; human gastric cancer cell line HGC-27; normal human gastric mucosal epithelial cell line GES-1 (provided by the Key Laboratory of Traditional Chinese Medicine Syndrome Signal Transduction in Henan Province).

[0066] 1.2 Reagents

[0067] Thiazole blue (MTT), supplied by Beijing Solarbio Technology Co., Ltd., item number M8180.

[0068] 2 methods

[0069] 2.1 Cell Culture

[0070] AGS cells, HGC-27 cells, and GES-1 cells were cultured in 1640 medium containing 10% (v / v) fetal bovine serum (FBS), 5958 mg / L 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES), 100 U / mL penicillin, and 100 μg / mL streptomycin, respectively. The cells were placed in an incubator at 37°C and 5% CO2 humidity. The cells were passaged every 1–2 days. All experiments used logarithmic cell growth.

[0071] 2.2 Observation of the growth status of gastric cancer cells in the normal group and the traditional Chinese medicine group

[0072] The three cell lines from section 2.1 were cultured to the logarithmic growth phase. When they covered more than 70% of a 100 mm culture dish, they were digested with trypsin containing disodium ethylenediaminetetraacetate (EDTA) and diluted with 1.2 × 10⁻⁶ mol / L. 5 Inoculate the culture medium at a density of 100 cells / mL into 6-well plates, setting up a control group and a treatment group (the traditional Chinese medicine formula in Example 1). Add the medicine every other day. For the control group, add 2 mL / well of 1640 medium containing 10% (v / v) fetal bovine serum directly. For the treatment group, add 2 mL / well of 1640 medium containing 10% (v / v) fetal bovine serum of the same concentration of traditional Chinese medicine extract (the traditional Chinese medicine formula in Example 1) as described in section 2.3. Continue culturing for 48 hours. Observe and record the results under a microscope.

[0073] 2.3 MTT assay for dose-response relationship of cell growth inhibition

[0074] AGS cells, HGC-27 cells, and GES-1 cells were 5 × 10⁵ 3 200 μL of each cell was seeded into a 96-well plate and cultured at 37°C in a 5% CO2 incubator for 24 h. After 24 h, when the cells were fully adherent, the culture medium in the wells was discarded. Five groups were set up, including a control group and a traditional Chinese medicine group, with three replicates in each group. The control group was supplemented with 200 μL of 1640 medium containing 10% (v / v) fetal bovine serum. The five traditional Chinese medicine groups were supplemented with 200 μL of traditional Chinese medicine extracts (the traditional Chinese medicine formula in Example 1, with 10% (v / v) fetal bovine serum in 1640 medium) at concentrations of 25, 50, 100, 200, and 400 μg / mL, respectively. The extracts were added slowly along the well walls. Continue culturing for 48 hours, remove the culture supernatant, add 100 mL of 0.5 mg / mL MTT solution to each well, continue culturing for 4 hours, carefully aspirate the supernatant, and add 150 μL of dimethyl sulfoxide (DMSO) to dissolve the purple precipitate. Measure the OD value using a microplate reader at a wavelength of 490 nm. (Follow the formula:)

[0075] Inhibition rate (%) = (1 - average A of the Chinese herb wells) 490值 / Average A of the control well 490值 )×100%

[0076] IC30 Value and IC 50 Value calculation: After repeating the above experiment three or more times, the obtained experimental data (cell inhibition rate) were imported into SPSS for analysis and calculation.

[0077] 2.4 Cell scratch assay to detect cell migration ability

[0078] AGS cells were loaded at 6 × 10 5 Cells / mL were seeded into 6-well plates with pre-marked lines on the bottom, with 3 replicates per well. Cells were incubated at 37°C and 5% CO2 for 24 hours until a monolayer was formed. Using a sterile 200μL pipette tip, a straight line was drawn from top to bottom along the center of each well. The supernatant and floating cells were removed. Cells were washed three times with PBS buffer. The control group received 1640 medium containing 2% (v / v) fetal bovine serum. The treatment group was screened according to the IC50 assay results in section 2.3. 30 (Drug concentration at which inhibition rate reaches 30%), add 2% (v / v) fetal bovine serum containing 20 μg / mL, 40 μg / mL, and 80 μg / mL of traditional Chinese medicine extract (formula from Example 1), and incubate in a 37°C, 5% CO2 incubator. Photographs are taken at 0h, 24h, and 48h. Before each photograph, the liquid is removed, and the cells are gently washed 1-2 times with PBS buffer, then the liquid is replaced to remove floating cells and avoid affecting the objectivity and aesthetics of the photographs. The experiment is repeated three times. The migration area is calculated using ImageJ software. The scratch healing rate is calculated using the formula:

[0079] Scratch healing rate (%) = (0h - 24h / 48h) / 0h × 100%

[0080] 2.5 Statistical Methods

[0081] Experimental data were tested for homogeneity of variance and normality using SPSS 25.0 software. The results are presented in [data missing]. This indicates that the application software GraphPad Prism 8 is used for plotting and analysis.

[0082] 3 Experimental Results

[0083] 3.1 Effects of Traditional Chinese Medicine Formulas on the Growth and Morphology of Human Gastric Cancer Cell Lines AGS, HGC-27, and GES-1

[0084] Microscopic observation revealed that cells in the control group adhered to the wall, with clear cell boundaries, obvious mitotic figures, and protruding pseudopodia; cells in the drug-treated group had very distinct outlines, extremely coarse cytoplasm filled with numerous granular substances, and with increasing drug concentration, extensive necrotic blank fields and vacuolated areas appeared (see...). Figure 1 This indicates that the condition of tumor cells deteriorates after medication, and some even die off in large numbers.

[0085] 3.2 Effects of Traditional Chinese Medicine Formulas on Tumor Cell Growth (Inhibition Rate)

[0086] The traditional Chinese medicine formula can inhibit the growth of human gastric cancer cell lines AGS and HGC-27, and the inhibition of cell growth shows a dose-response relationship with increasing drug concentration (see Table 1 and 2). Figure 2 High doses of traditional Chinese medicine (TCM) drugs have a strong inhibitory effect on all cell types, but at a certain concentration, the inhibition rate on the normal human gastric mucosal epithelial cell line GES-1 is low. This indicates that the TCM formula has a high killing effect on cancer cells but a lower killing effect on normal cells, with fewer side effects. Simultaneously, calculations show that the IC50 of AGS at 24 hours... 30 The value was 52 μg / mL, IC50 50 The value was 142 μg / mL, and the IC50 of HGC-27 was... 30 The value was 102 μg / mL, IC50 50 The value was 195 μg / mL, and the IC50 of GES-1 was... 30 The value was 203 μg / mL, IC50 50 The value was 269 μg / mL; the IC50 of AGS at 48 h was... 30 The value was 38 μg / mL, IC50 50 The value was 120 μg / mL, and the IC50 of HGC-27 was... 30 The value was 57 μg / mL, IC50 50 The value is 150 μg / mL, and the IC50 of GES-1 is... 30 The value was 157 μg / mL, IC50 50 The value was 158 μg / mL.

[0087] Table 1: Changes in the inhibition rate of tumor cell growth by traditional Chinese medicine with drug concentration (unit: μg / mL)

[0088]

[0089] Note: Compared with 0 μg / mL in each group, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

[0090] 3.3 Scratch test to detect the effect of traditional Chinese medicine formula on the proliferation of AGS gastric cancer cells

[0091] After scratching and drug application, images were taken at 0h, 24h, and 48h. The results of the cell scratch assay showed that the wound healing rate of AGS cell migration decreased with increasing drug concentration (see...). Figure 3 (See Table 2).

[0092] Table 2: Comparison of scratch healing rates of AGS cells with different concentrations of traditional Chinese medicine formulations ( n=3)

[0093]

[0094] Note: Compared with 0 μg / mL in each group, **P < 0.01, ***P < 0.001, ****P < 0.0001.

[0095] 4. Conclusion

[0096] 4.1 The experimental results of the traditional Chinese medicine formulation for anti-gastric cancer showed a good anti-gastric cancer effect, which was dose-dependent.

[0097] 4.2 The traditional Chinese medicine formula for treating gastric cancer can effectively inhibit the growth of gastric cancer cells AGS and HGC-27 in a dose-dependent manner, and has little killing effect on normal gastric mucosal epithelial cells GES-1. This drug formula has a good effect on inhibiting tumor growth and has few side effects.

[0098] 4.3 By observing and quantifying the ability of gastric cancer cells to migrate to blank areas, it was found that the migration ability of gastric cancer cells treated with the traditional Chinese medicine formula decreased significantly in a dose-dependent manner. The traditional Chinese medicine formula for treating gastric cancer has a good anti-gastric cancer effect and low toxicity, representing an innovation in gastric cancer treatment drugs and possessing significant clinical significance.

Claims

1. A traditional Chinese medicine composition for treating gastric cancer, characterized in that, It is made from the following raw materials in parts by weight: Angelica dahurica 3-9 parts, Coptis chinensis 3-9 parts, Panax ginseng 3-9 parts, Hedyotis diffusa 15-30 parts, Ziziphus jujuba var. spinosa 10-15 parts, Polygonum cuspidatum 9-15 parts, and Lindera strychnifolia 3-9 parts.

2. The traditional Chinese medicine composition for treating gastric cancer according to claim 1, characterized in that, It is made from the following raw materials in parts by weight: Angelica dahurica 3 parts, Coptis chinensis 3 parts, ginseng 3 parts, Hedyotis diffusa 15 parts, Aconitum carmichaelii 10 parts, Polygonum cuspidatum 9 parts, and Lindera strychnifolia 3 parts.

3. A method for preparing a traditional Chinese medicine composition for treating gastric cancer as described in claim 1 or 2, characterized in that, Each raw material is extracted with ethanol separately, then dried, and the dried extracts are then mixed together to obtain the final product.

4. The preparation method according to claim 3, characterized in that, Specifically, the following steps are included: (1) Clean the Angelica dahurica, Coptis chinensis, ginseng, Hedyotis diffusa, Ziziphus jujuba, Polygonum cuspidatum, and Lindera strychnifolia, chop them, and grind them into coarse powder. (2) Add ethanol solution to each raw material, soak at room temperature in the dark, then add to a reflux flask for extraction, boil, filter, take the filtrate, recover the residue, repeat the above extraction steps, and take the filtrate. (3) Mix the two filtrates of each raw material separately, put them on a rotary evaporator to remove ethanol, then evaporate the water in a water bath, freeze them in a refrigerator, freeze-dry them, and finally pulverize them into powder. (4) Mix the powdered raw materials together to obtain the final product.

5. The preparation method according to claim 4, characterized in that, In step (2), the volume concentration of the ethanol solution is 70-75%, and the amount used is 8-10 times the volume of the raw drug.

6. The preparation method according to claim 4, characterized in that, The soaking time in step (2) is 24-48 hours.

7. The preparation method according to claim 4, characterized in that, The reflux temperature in step (2) is 90-100℃.

8. The preparation method according to claim 4, characterized in that, In step (3), the evaporation temperature of the rotary evaporator is 42-50℃.

9. The preparation method according to claim 3, characterized in that, In step (3), the water bath temperature is 70-80℃.

10. The use of a traditional Chinese medicine composition as described in claim 1 or 2 in the preparation of a drug for treating gastric cancer.

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