Detection method and system based on flexible microwell plate and electronic equipment

Abstract

The invention discloses a detection method and system based on a flexible microwell plate and electronic equipment, and relates to the technical field of microwell plates. The bottom of the flexible microplate is provided with a film, and the method comprises the following steps: determining a target micropore on the flexible microplate, and determining at least one reference point on the target micropore; moving the detection device to move the view center of the detection device to the reference point theoretical position of the reference point; obtaining a reference point actual position of the reference point according to the position deviation between the reference point and the view center; obtaining the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore; the detection device is moved until the view center is located at the actual center position so as to detect the to-be-detected object in the target thin film area, and the target thin film area is the thin film area corresponding to the target micropore.

Description

Technical Field

[0001] The present invention relates to the technical field of micro-well plates, and in particular to a detection method, system and electronic equipment based on a flexible micro-well plate. Background Art

[0002] Microplate bioassay technology is a high-throughput screening technology that is widely used in drug discovery, biomedical research, disease diagnosis and other fields. The basic principle of this technology combines the characteristics of solid phase adsorption, enzyme-linked immunosorbent assay and other biomolecular interaction analysis methods, using microplates as reaction carriers to detect and quantitatively analyze target substances through specific biomolecular recognition events.

[0003] However, the related art technology based on microplate detection does not take into account the influence of the microplate itself on the detection, so that the detection accuracy is limited. Summary of the invention

[0004] The present invention aims to solve one of the technical problems in the related art to at least a certain extent. To this end, the first object of the present invention is to propose a detection method based on a flexible microplate to avoid the influence of the flexible microplate on the detection and improve the detection accuracy.

[0005] A second objective of the present invention is to provide an electronic device.

[0006] The third object of the present invention is to provide a detection system based on a flexible microplate.

[0007] To achieve the above-mentioned purpose, the first aspect of the present invention implements a detection method based on a flexible microporous plate, wherein a thin film is arranged at the bottom of the flexible microporous plate, and the method comprises: determining a target micropore on the flexible microporous plate, and determining at least one reference point on the target micropore; moving a detection device to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point; obtaining the actual position of the reference point of the reference point according to the position deviation between the reference point and the center of the field of view; obtaining the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore; moving the detection device until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target thin film area, wherein the target thin film area is the thin film area corresponding to the target micropore.

[0008] In addition, the detection method based on the flexible microplate according to the embodiment of the present invention may also have the following additional technical features:

[0009] In one embodiment of the present invention, the actual center position of the target micropore is obtained according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore, including: for each of the reference points, the theoretical coordinates of the theoretical position of the reference point in a preset coordinate system and the actual coordinates of the actual position of the reference point in the preset coordinate system are obtained, and the coordinate difference between the theoretical coordinates and the actual coordinates is obtained; a coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size; the theoretical center coordinates of the theoretical center position in the preset coordinate system are obtained, the theoretical center coordinates are corrected according to the coordinate correction value, and the actual center position is obtained according to the corrected theoretical center coordinates.

[0010] In one embodiment of the present invention, the field of view of the detection device is a square field of view with a side length of a first length, the reference point includes a first reference point, the coordinate difference includes a first x-axis coordinate difference and a first y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the first reference point in the preset coordinate system, and the coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: obtaining a first product of the first x-axis coordinate difference and the resolution, and obtaining a second product of the first y-axis coordinate difference and the resolution; dividing the first product by the first length to obtain the x-axis coordinate correction value, and dividing the second product by the first length to obtain the y-axis coordinate correction value.

[0011] In one embodiment of the present invention, the field of view of the detection device is a square field of view with a side length of a second length, the reference points include a second reference point and a third reference point, the coordinate difference includes a second x-axis coordinate difference and a second y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the second reference point in the preset coordinate system, and also includes a third x-axis coordinate difference and a third y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the third reference point in the preset coordinate system, wherein a line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, and the coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: when the first distance between the second reference point and the center of the micropore is equal to the second distance between the third reference point and the center of the micropore, according to the second x-axis coordinate difference, The coordinate correction value is obtained by the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the second length and the resolution; when the first distance is not equal to the second distance, the fourth x-axis coordinate difference and the fourth y-axis coordinate difference between the second reference point and the center of the microhole in the preset coordinate system are obtained, and the fifth x-axis coordinate difference and the fifth y-axis coordinate difference between the third reference point and the center of the microhole in the preset coordinate system are obtained, and the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fourth y-axis coordinate difference, the fifth x-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

[0012] In one embodiment of the present invention, the coordinate correction value includes an x-axis coordinate correction value and a y-axis coordinate correction value, and the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the second length and the resolution, including: obtaining a first mean of the second x-axis coordinate difference and the third x-axis coordinate difference, and obtaining a second mean of the second y-axis coordinate difference and the third y-axis coordinate difference; multiplying the first mean by the second length, and dividing the multiplication result by the resolution, to obtain the x-axis coordinate correction value; multiplying the second mean by the second length, and dividing the multiplication result by the resolution, to obtain the y-axis coordinate correction value.

[0013] In one embodiment of the present invention, the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fourth y-axis coordinate difference, the fifth x-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution, including: when the fourth y-axis coordinate difference and the fifth y-axis coordinate difference are both zero, according to the second x-axis coordinate difference, the second y-axis coordinate difference, the fourth x-axis coordinate difference, the fifth y-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution The coordinate correction value is obtained according to the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fifth x-axis coordinate difference, the second length and the resolution; when the fourth x-axis coordinate difference and the fifth x-axis coordinate difference are both zero, the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth y-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

[0014] In one embodiment of the present invention, the moving detection device to move the center of the field of view of the detection device to the reference point theoretical position of the reference point includes: moving the detection device according to the theoretical center position of the target micropore to move the center of the field of view to the theoretical center position; determining a moving path according to the size of the target micropore, and moving the detection device according to the moving path so that the center of the field of view after movement is located at the reference point theoretical position.

[0015] In one embodiment of the present invention, the mobile detection device is used to move the center of the field of view of the detection device to the reference point theoretical position of the reference point, including: determining the reference point theoretical position according to the theoretical center position and size of the target micropore; and moving the detection device according to the reference point theoretical position so that the center of the field of view after movement is located at the reference point theoretical position.

[0016] To achieve the above objectives, the second aspect of the present invention implements an electronic device, including a memory, a processor, and a computer program stored in the memory and running on the processor. When the computer program is executed by the processor, the above-mentioned flexible microplate-based detection method is implemented.

[0017] To achieve the above-mentioned purpose, the third aspect of the present invention implements and proposes a detection system based on a flexible microporous plate, including a flexible microporous plate, a detection device, a moving device and a controller, wherein a film is arranged at the bottom of the flexible microporous plate, and the controller includes the above-mentioned electronic device; wherein the moving device is connected to the detection device and the controller to move the detection device under the control of the controller, and the detection device is connected to the controller to detect the object to be detected in the target film area under the control of the controller, and the target film area is a film area corresponding to the preset target micropores on the flexible microporous plate.

[0018] According to the detection method, system and electronic device based on the flexible microporous plate of the embodiment of the present invention, the target microporous plate is determined, and at least one reference point on the target microporous plate is determined; the detection device is moved to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point; the actual position of the reference point of the reference point is obtained according to the position deviation between the reference point and the center of the field of view; the actual center position of the target microporous is obtained according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target microporous; the detection device is moved until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target microporous. In this way, the deformation of the flexible microporous plate can be avoided from affecting the detection, thereby realizing accurate detection of the object to be detected in the target microporous area.

[0019] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a flow chart of a detection method based on a flexible microplate according to an embodiment of the present invention;

[0021] Figure 2 is a schematic diagram of a detection method based on a flexible microplate according to an example of the present invention;

[0022] Figure 3 is a schematic diagram of a flexible microporous plate according to an example of the present invention;

[0023] Figure 4 It is a result schematic diagram of a detection method based on a flexible microplate according to an example of the present invention;

[0024] Figure 5 is a result schematic diagram of another example of a detection method based on a flexible microplate of the present invention;

[0025] Figure 6 It is a result schematic diagram of another example of a detection method based on a flexible microplate of the present invention;

[0026] Figure 7 is a structural block diagram of an electronic device according to an embodiment of the present invention;

[0027] Figure 8 It is a structural block diagram of a detection system based on a flexible microplate according to an embodiment of the present invention. DETAILED DESCRIPTION

[0028] The following describes the detection method, system and electronic device based on the flexible microplate according to the embodiments of the present invention with reference to the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described with reference to the accompanying drawings are exemplary and should not be construed as limiting the present invention.

[0029] Figure 1 It is a flow chart of a detection method based on a flexible microplate according to an embodiment of the present invention.

[0030] In the embodiment of the present invention, a thin film is disposed at the bottom of the flexible microporous plate.

[0031] The flexible microporous plate is a plate provided with micropores, and a film is provided at the bottom of the flexible microporous plate.

[0032] See also Figure 2 The example shown in the figure shows that the film is a silicon nitride film. The silicon nitride film has good light transmittance and is suitable for precise measurement scenarios, such as the need to accurately locate the height of the film and the sample through the diffraction ring. Figure 2 It can be seen that micropores are arranged on the orifice plate, and the silicon nitride film is arranged at the bottom of the flexible micropore plate.

[0033] The flexible microporous plate is made of a flexible material, such as a rubber material. The micropores of the flexible microporous plate can be regular or irregular in shape. Figure 3 The example shown in Figure 3 In the flexible microporous plate, the micropores are square.

[0034] By adopting the above-mentioned method of attaching a silicon nitride film to the bottom of the flexible microporous plate, not only can the silicon nitride film window be adaptively leveled during the film pressing process, significantly reducing the risk of film breakage, but also improve the redundancy of the control accuracy of the diamond height and the moving distance of the objective lens. In addition, the flexible sealing structure is also conducive to the expansion of the focus range, so the robustness and stability of the detection scheme can be significantly improved. For example, since the silicon nitride film is arranged at the bottom of the flexible microporous plate, the flexible microporous plate will play a buffering role during film pressing, so that the force is evenly applied to the silicon nitride film, thereby reducing the risk of film breakage; and, due to the buffering capacity of the flexible microporous plate, the vibration of the various components in the detection device, such as diamonds, objective lenses, etc., when the detection device is moved can be reduced, thereby improving the control accuracy; and, due to the use of flexible materials, the influence of factors such as thermal expansion and contraction of materials and wear of mechanical parts on the focus range can be offset to a certain extent, thereby facilitating the expansion of the focus range.

[0035] However, due to the use of flexible microplates, during testing, due to the deformation of the microplate, the position of the micropores will inevitably have errors in the xyz direction relative to the original design value of the microplate. Among them, the error in the z direction is small and can be solved by adjusting the diamond height, focusing, etc., but the error in the xy direction is relatively large. According to actual measurements, the error can reach more than 200μm.

[0036] The deformation of the flexible microporous plate will have a negative impact on the detection. For example, after the film is inflated and pressurized, there is a height difference between the edge and the center of the film. If the center of the objective lens is facing the edge of the film, it is difficult to ensure that the axis of the detection light path is perpendicular to the plane of the film, thus causing measurement errors.

[0037] Therefore, the present application proposes a detection method based on a flexible microporous plate to avoid the deformation of the flexible microporous plate affecting the detection when the flexible microporous plate is used.

[0038] like Figure 1 As shown, the detection method based on the flexible microplate comprises:

[0039] S11, determining a target microwell on the flexible microwell plate, and determining at least one reference point on the target microwell.

[0040] Specifically, in order to perform detection based on the flexible microwell plate, firstly, a target microwell on the flexible microwell plate is determined, and the target microwell is a microwell that needs to be detected.

[0041] In order to offset the influence of the deformation of the flexible micro-well plate on the detection structure, after determining the target micro-well, it is also necessary to determine the reference point on the target micro-well, so as to use the reference point to compensate for the influence of the deformation of the flexible micro-well plate on the detection structure.

[0042] S12, moving the detection device to move the field of view center of the detection device to the reference point theoretical position of the reference point.

[0043] Specifically, when setting the flexible micro-well plate, it is necessary to preset the position of the flexible micro-well plate, predetermine the reference point corresponding to each micro-well on the flexible micro-well plate, and predetermine the position of the reference point on the corresponding micro-well. Therefore, after determining the target micro-well, the reference point of the target micro-well can be determined, and the theoretical position of the reference point during design can be obtained, which is the theoretical position of the reference point. In other words, the theoretical position of the reference point is the position where the reference point should be located during pre-design.

[0044] Therefore, after obtaining the theoretical position of the reference point, the visual field center of the detection device can be moved to the theoretical position of the reference point.

[0045] When moving the detection device, the detection device may be moved as a whole, or only a part of the reference device may be moved. For example, only the objective lens of the reference device may be moved.

[0046] S13, obtaining the actual position of the reference point of the reference point according to the position deviation between the reference point and the center of the field of view.

[0047] Specifically, after the center of the field of view of the detection device is moved to the theoretical position of the reference point, since the deformation of the flexible microporous plate is usually not too large, that is, after the center of the field of view of the detection device is moved to the theoretical position of the reference point, even if the reference point is not in the center of the field of view of the detection device, it will usually appear in the field of view of the detection device. Therefore, the additional process of finding the reference point can be omitted, and the reference point can be directly found according to the observation result of the detection device, and then the position deviation between the reference point and the center of the field of view of the detection device is obtained, so that the actual position of the reference point is obtained according to the position deviation.

[0048] S14, obtaining the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore.

[0049] Specifically, after obtaining the theoretical position and actual position of the reference point, the deformation of the target micropore part on the flexible micropore plate can be inferred based on the theoretical position and actual position of the reference point, so that the theoretical center position of the target micropore can be corrected according to the deformation to obtain the actual center position of the target micropore.

[0050] S15, moving the detection device until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target micropore.

[0051] Specifically, since the flexible microporous plate has a film attached to the bottom, the film needs to be inflated and pressurized, the film height at the edge of each microporous is inconsistent with the film height at the center of the microporous, that is, for each microporous, there is a height difference of the film in the film area corresponding to the microporous. Therefore, when the center of the field of view of the detection device is aligned with the center of the target microporous, the optical path of the detection device will be perpendicular to the film, thereby achieving the best detection effect.

[0052] Therefore, after determining the actual center position of the target micropore, the detection device can be moved until the center of the field of view of the detection device is located at the actual center position of the target micropore, and then the object to be detected in the target film area corresponding to the target micropore can be detected. Figure 3 In the example shown, since the film is disposed at the bottom of the flexible microporous plate, the target film area is the film area that can be observed through the target micropores from a top-down perspective.

[0053] In this way, the deformation of the flexible microporous plate can be prevented from affecting the detection, thereby achieving the best detection effect.

[0054] In some embodiments of the present invention, the actual center position of the target microhole is obtained according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target microhole, including: for each reference point, the theoretical coordinates of the theoretical position of the reference point in a preset coordinate system and the actual coordinates of the actual position of the reference point in the preset coordinate system are obtained, and the coordinate difference between the theoretical coordinates and the actual coordinates is obtained; the coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size; the theoretical center coordinates of the theoretical center position in the preset coordinate system are obtained, and the theoretical center coordinates are corrected according to the coordinate correction value, and the actual center position is obtained according to the corrected theoretical center coordinates.

[0055] Specifically, firstly, the theoretical coordinates of the theoretical position of the reference point and the actual coordinates of the actual position of the reference point are obtained, and then the coordinate difference between the theoretical coordinates and the actual coordinates can be obtained.

[0056] After obtaining the coordinate difference, the coordinate difference can be corrected according to the field of view size and resolution of the detection device, and the deformation degree of the flexible microwell plate can be obtained according to the correction result, so as to correct the theoretical center coordinates of the target microwell according to the deformation degree and obtain the actual center coordinates of the flexible microwell plate.

[0057] Therefore, after obtaining the coordinate difference between the theoretical coordinates and the actual coordinates, the coordinate difference is corrected according to the parameters of the detection device, thereby further improving the accuracy of the detection.

[0058] In some embodiments of the present invention, the field of view of the detection device is a square field of view with a side length of a first length, the reference point includes a first reference point, the coordinate difference includes a first x-axis coordinate difference and a first y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the first reference point in a preset coordinate system, and a coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: obtaining a first product of the first x-axis coordinate difference and the resolution, and obtaining a second product of the first y-axis coordinate difference and the resolution; dividing the first product by the first length to obtain an x-axis coordinate correction value, and dividing the second product by the first length to obtain a y-axis coordinate correction value.

[0059] The following is an explanation with reference to specific examples.

[0060] In the first example, the target microwell is a square.

[0061] Specifically, the design value coordinates of the center of the target microhole are (x0, y0), the microhole is a square with a side length of 2a, the field of view is a square with a side length of 2b and a resolution of 2c×2c is adopted, a is greater than b, that is, the side length of the field of view is 2b, the resolution is 2c, and the two vertical sides of the microhole and the field of view are respectively set along the xy direction of the translation stage, then:

[0062] First, move the stage to align the center of the field of view to (x0-a, y0-a);

[0063] The relative position of the micropore corner and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx, Δy);

[0064] Then the coordinates of the micropore center are corrected to Then move the translation stage to align the center of the objective lens field of view with the center of the film window.

[0065] That is, in this example, the first length is 2a, the field of view size is 2b, the theoretical coordinates of the first reference point are (x0-a, y0-a), and the first x-axis coordinate difference is The first y-axis coordinate difference is

[0066] In the second example, the target microwells are of irregular shape.

[0067] Specifically, the design value coordinates of the target micro-hole center are (x0, y0), the field of view is a square with a side length of 2b and a resolution of 2c×2c, that is, the field of view has a side length of 2b and a resolution of 2c. A positioning correction mark can be set on the edge of the micro-hole of the well plate, and the displacement coordinates (m, n) between the mark and the center of the micro-hole relative to the displacement stage need to be known in advance, that is, the theoretical coordinates of the reference point of the first reference point are (x0+m, y0+n):

[0068] First, move the stage to align the center of the field of view to (x0+m, y0+n);

[0069] The relative position of the micropore corner and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx, Δy);

[0070] Then the coordinates of the micropore center are corrected to Then move the translation stage to align the center of the objective lens field of view with the center of the film window.

[0071] The above-mentioned film window is the target micropore.

[0072] It should be noted that although Δx and Δy are used in the above two examples, it is only to indicate that Δx is the coordinate difference in the first x-axis direction and Δy is the coordinate difference in the first y-axis direction, and it does not mean that their specific values ​​are the same in different cases.

[0073] In some embodiments of the present invention, since in actual applications the position deviation of a reference point cannot be completely equal to the position deviation of the microhole center, it is necessary to obtain the position deviations of at least two reference points to obtain the accurate position deviation of the microhole center.

[0074] Therefore, the field of view of the detection device is set to a square field of view with a side length of the second length, the reference points include the second reference point and the third reference point, the coordinate difference includes the second x-axis direction coordinate difference and the second y-axis direction coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the second reference point in the preset coordinate system, and also includes the third x-axis direction coordinate difference and the third y-axis direction coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the third reference point in the preset coordinate system, wherein a line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, and a coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: when the first distance between the second reference point and the micropore center is equal to the second distance between the third reference point and the micropore center, according to the second x-axis direction coordinate difference , the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the second length and the resolution are used to obtain the coordinate correction value; when the first distance is not equal to the second distance, the fourth x-axis coordinate difference and the fourth y-axis coordinate difference between the second reference point and the center of the microhole in the preset coordinate system are obtained, and the fifth x-axis coordinate difference and the fifth y-axis coordinate difference between the third reference point and the center of the microhole in the preset coordinate system are obtained, and the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fourth y-axis coordinate difference, the fifth x-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

[0075] Among them, the coordinate correction value includes an x-axis coordinate correction value and a y-axis coordinate correction value, and the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the second length and the resolution, including: obtaining a first mean of the second x-axis coordinate difference and the third x-axis coordinate difference, and obtaining a second mean of the second y-axis coordinate difference and the third y-axis coordinate difference; multiplying the first mean by the second length, and dividing the multiplication result by the resolution to obtain the x-axis coordinate correction value; multiplying the second mean by the second length, and dividing the multiplication result by the resolution to obtain the y-axis coordinate correction value.

[0076] Obtaining a coordinate correction value according to a second x-axis coordinate difference, a second y-axis coordinate difference, a third x-axis coordinate difference, a third y-axis coordinate difference, a fourth x-axis coordinate difference, a fourth y-axis coordinate difference, a fifth x-axis coordinate difference, a fifth y-axis coordinate difference, a second length and a resolution, including: when the fourth y-axis coordinate difference and the fifth y-axis coordinate difference are both zero, obtaining a coordinate correction value according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fifth x-axis coordinate difference, the second length and the resolution; when the fourth x-axis coordinate difference and the fifth x-axis coordinate difference are both zero, obtaining a coordinate correction value according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth y-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

[0077] The second length may be the same as the first length.

[0078] The following is an explanation based on the first example.

[0079] In this example, the design value coordinates of the center of the target micropore are (x0, y0), the micropore is a square with a side length of 2a, the field of view is a square with a side length of 2b and a resolution of 2c×2c is adopted, a is greater than b, that is, the field of view has a side length of 2b, the resolution is 2c, the target micropore is a square micropore, and the second reference point and the third reference point are two vertices on the micropore in this direction. Since the line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, it can be clearly seen that the first distance between the second reference point and the micropore center is equal to the second distance between the third reference point and the micropore center. The theoretical coordinate positions of the second reference point and the third reference point are (x0-a, y0-a) and (x0+a, y0+a), respectively.

[0080] At this time, first move the translation stage to align the center of the field of view to (x0-a, y0-a);

[0081] The relative position of the micropore corner (x0-a, y0-a) and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx1, Δy1), which is the coordinate difference in the second x-axis direction and the coordinate difference in the second y-axis direction.

[0082] First, move the stage to align the center of the field of view to (x0+a, y0+a);

[0083] The relative position of the micropore corner (x0+a, y0+a) and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx2, Δy2), which is the coordinate difference in the third x-axis direction and the coordinate difference in the third y-axis direction.

[0084] Then the coordinates of the micropore center are corrected to Then move the stage so that the center of the objective lens field of view is aligned with the center of the film window. In other words, the coordinate correction value is and

[0085] The following is an explanation with reference to the second example.

[0086] In this example, the design value coordinates of the center of the target micropore are (x0, y0), the field of view is a square with a side length of 2b and a resolution of 2c×2c, that is, the field of view has a side length of 2b and a resolution of 2c. The target micropore is an irregular micropore, and the line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, and the first distance between the second reference point and the micropore center is equal to the second distance between the third reference point and the micropore center.

[0087] At this time, the displacement coordinates between the second reference point and the third reference point and the center of the microhole relative to the displacement stage are (m, n) and (-m, -n), that is, the theoretical reference point coordinates of the second reference point and the third reference point are (x0-m, y0-n) and (x0+m, y0+n), respectively.

[0088] First, move the stage to align the center of the field of view to (x0-m, y0-n);

[0089] The relative position between the reference point and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx1, Δy1), which is the coordinate difference in the second x-axis direction and the coordinate difference in the second y-axis direction.

[0090] First, move the stage to align the center of the field of view to (x0+m, y0+n);

[0091] The relative position between the reference point and the center of the field of view (the center of the microhole is the origin of the field of view coordinate system) is (Δx2, Δy2), which is the coordinate difference in the third x-axis direction and the coordinate difference in the third y-axis direction.

[0092] Then the coordinates of the micropore center are corrected to Then move the translation stage to align the center of the objective lens field of view with the center of the film window.

[0093] The following is an explanation with reference to the third example.

[0094] In this example, the design value coordinates of the center of the target micropore are (x0, y0), the field of view is a square with a side length of 2b and a resolution of 2c×2c, that is, the field of view has a side length of 2b and a resolution of 2c. The target micropore is an irregular micropore, and the line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, and the first distance between the second reference point and the micropore center is not equal to the second distance between the third reference point and the micropore center.

[0095] At this time, the displacement coordinates between the two marks and the center of the microhole relative to the displacement stage are (m, n) and (-m', -n'), that is, the theoretical coordinates of the reference points of the second reference point and the third reference point are (x0-m', y0-n') and (x0+m, y0+n), respectively.

[0096] First, move the stage to align the center of the field of view to (x0-m', y0-n');

[0097] The relative position between the reference point and the center of the field of view (the center of the micropore is the origin of the field of view coordinate system) is (Δx1, Δy1), which is the coordinate difference in the second x-axis direction and the coordinate difference in the second y-axis direction.

[0098] First, move the stage to align the center of the field of view to (x0+m, y0+n);

[0099] The relative position between the reference point and the center of the field of view (the center of the microhole is the origin of the field of view coordinate system) is (Δx2, Δy2), which is the coordinate difference in the third x-axis direction and the coordinate difference in the third y-axis direction.

[0100] The actual center position is or

[0101] At this time, if m and m' are both 0, that is, the coordinate difference in the fourth x-axis direction and the coordinate difference in the fifth x-axis direction are both zero, then the actual center position is

[0102] If n and n' are both 0, that is, the coordinate difference in the fourth y-axis direction and the coordinate difference in the fifth y-axis direction are both zero, then the actual center position is

[0103] If m and m' are not both 0, and n and n' are not both 0, then the actual center position is or

[0104] It should be noted that although Δx1, Δy1, Δx2, and Δy2 are used in the above three examples, it does not mean that their specific values ​​are the same in different cases. They are only for the convenience of indicating that they are the coordinate difference in the second x-axis direction, the coordinate difference in the second y-axis direction, the coordinate difference in the third x-axis direction, and the coordinate difference in the third y-axis direction, respectively.

[0105] It should also be noted that when the target micropore is an irregular micropore, in order to facilitate observation, the reference point can be set as the corner of the micropore, thereby facilitating observation and further improving detection accuracy.

[0106] In some embodiments of the present invention, the detection device is moved to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point, including: moving the detection device according to the theoretical center position of the target micropore to move the center of the field of view to the theoretical center position; determining a moving path according to the size of the target micropore, and moving the detection device according to the moving path so that the center of the field of view after movement is located at the theoretical position of the reference point.

[0107] Specifically, first move the center of the field of view of the detection device to the theoretical center position of the target micropore. Since the reference point has been set in advance, the theoretical position of the reference point can be calculated according to the size of the target micropore. For example, if the target micropore is a square and the reference point is set in advance as a vertex on the target micropore, the theoretical position of the reference point can be calculated according to the size of the target micropore.

[0108] After calculating the theoretical position of the reference point, the moving path can be determined according to the theoretical position of the reference point and the current position of the center of the field of view, so that the detection device is moved according to the moving path so that the center of the field of view after movement is located at the theoretical position of the reference point.

[0109] The following is an explanation with an example.

[0110] Specifically, the system includes an optical system (objective lens + camera), a plate with a number of micro-holes arranged in a matrix, and a displacement stage that can accurately measure and control the displacement. The micro-holes are rectangular. The positioning error correction method is:

[0111] Step 1: First, move the stage to move the center of the objective lens to the designed coordinate position of the target micro-hole center (due to the deformation of the micro-hole plate, the center of the objective lens is not really aligned with the target micro-hole center at this time, but the field of view of the objective lens is only 1.3x1.3mm, and the film window is 2x2 mm. At this time, the film window boundary cannot be observed in the field of view, so the positioning error cannot be corrected). At this time, you can refer to Figure 4 , the center of the field of view is located at the center of the microhole.

[0112] Step 2: Move the stage to move the center of the objective lens to the corner of the target micro-hole. According to the design specifications of the target micro-hole, calculate the coordinate position of the corner of the target micro-hole, and calculate the displacement of the stage in the xy direction according to the design value coordinates of the center of the target micro-hole, and execute the displacement in the xy direction. At this time, if the flexible micro-hole plate does not deform, the movement result should be as follows: Figure 5 , the center of the field of view of the detection device is located at the reference point. If the flexible microplate is deformed, the movement result can be seen in Figure 6 , the center of the field of view of the detection device is not located at the reference point.

[0113] That is, in this example, the reference point is the corner of the target microvia.

[0114] Step 3: Determine the pixel coordinates of the corner of the target microhole in the field of view of the objective lens, and determine the displacement correction amount in the xy direction based on the difference between the corner pixel coordinates and the center pixel coordinates of the field of view of the objective lens.

[0115] Step 4: According to the displacement correction amount in the xy direction, move the translation stage to move the center of the objective lens to the center of the target micropore, and then perform film stretching and detection.

[0116] In some embodiments of the present invention, the detection device is moved to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point, including: determining the theoretical position of the reference point according to the theoretical center position and size of the target micropore; and moving the detection device according to the theoretical position of the reference point so that the center of the field of view after movement is located at the theoretical position of the reference point.

[0117] If the above example is used for explanation, the above steps 1 and 2 can be combined, that is, the coordinate position of the corner of the target microhole is directly calculated according to the design value coordinates of the center of the target microhole and the design specification size of the target microhole, the center of the objective lens is moved to the corner of the microhole, and then the displacement correction amount in the xy direction is calculated.

[0118] Thereby, the detection speed can be further improved.

[0119] In summary, the detection method based on the flexible microporous plate of the embodiment of the present invention determines the target micropore on the flexible microporous plate, and determines at least one reference point on the target micropore; moves the detection device to move the field of view center of the detection device to the reference point theoretical position of the reference point; obtains the reference point actual position of the reference point according to the position deviation between the reference point and the field of view center; obtains the actual center position of the target micropore according to the reference point theoretical position, the reference point actual position and the theoretical center position of the target micropore; moves the detection device until the field of view center is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target micropore. In this way, the deformation of the flexible microporous plate can be avoided from affecting the detection, thereby realizing accurate detection of the object to be detected in the target micropore area.

[0120] Furthermore, the present invention provides an electronic device.

[0121] Figure 7 It is a structural block diagram of an electronic device according to an embodiment of the present invention.

[0122] like Figure 7 As shown, the electronic device 500 includes: a processor 501 and a memory 503. The processor 501 and the memory 503 are connected, such as through a bus 502. Optionally, the electronic device 500 may also include a transceiver 504. It should be noted that in actual applications, the transceiver 504 is not limited to one, and the structure of the electronic device 500 does not constitute a limitation on the embodiments of the present invention.

[0123] Processor 501 may be a CPU (Central Processing Unit), a general purpose processor, a DSP (Digital Signal Processor), an ASIC (Application Specific Integrated Circuit), an FPGA (Field Programmable Gate Array) or other programmable logic devices, transistor logic devices, hardware components or any combination thereof. It may implement or execute various exemplary logic blocks, modules and circuits described in conjunction with the disclosure of the present invention. Processor 501 may also be a combination that implements computing functions, such as a combination of one or more microprocessors, a combination of a DSP and a microprocessor, etc.

[0124] The bus 502 may include a path to transmit information between the above components. The bus 502 may be a PCI (Peripheral Component Interconnect) bus or an EISA (Extended Industry Standard Architecture) bus. The bus 502 may be divided into an address bus, a data bus, a control bus, etc. For ease of representation, Figure 5 Only one thick line is used in the diagram, but this does not mean that there is only one bus or only one type of bus.

[0125] The memory 503 is used to store a computer program corresponding to the detection method based on the flexible microplate of the above embodiment of the present invention, and the computer program is controlled and executed by the processor 501. The processor 501 is used to execute the computer program stored in the memory 503 to implement the content shown in the above method embodiment.

[0126] in, Figure 7 The electronic device 500 shown is only an example and should not bring any limitation to the functions and scope of use of the embodiments of the present invention.

[0127] The electronic device of the embodiment of the present invention determines the target micropore on the flexible micropore plate and at least one reference point on the target micropore by implementing the detection method based on the flexible micropore plate of the above embodiment; moves the detection device to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point; obtains the actual position of the reference point of the reference point according to the position deviation between the reference point and the center of the field of view; obtains the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore; moves the detection device until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target micropore. In this way, the deformation of the flexible micropore plate can be avoided from affecting the detection, thereby realizing accurate detection of the object to be detected in the target micropore area.

[0128] Furthermore, the present invention proposes a detection system based on a flexible microplate.

[0129] Figure 8 It is a structural block diagram of a detection system based on a flexible microplate according to an embodiment of the present invention.

[0130] like Figure 8As shown, a detection system 100 based on a flexible microporous plate includes a flexible microporous plate 101, a detection device 102, a moving device 103 and a controller 104. A film is arranged at the bottom of the flexible microporous plate 101, and the controller 104 includes the above-mentioned electronic device 500; wherein the moving device 103 is connected to the detection device 102 and the controller 104 to move the detection device 102 under the control of the controller 104, and the detection device 102 is connected to the controller 104 to detect the object to be detected in the target film area under the control of the controller 104, and the target film area is the film area corresponding to the preset target micropores on the flexible microporous plate 101.

[0131] The detection system based on the flexible microporous plate of the embodiment of the present invention determines the target microporous plate on the flexible microporous plate and determines at least one reference point on the target microporous plate through the above-mentioned electronic device; moves the detection device to move the center of the field of view of the detection device to the theoretical position of the reference point of the reference point; obtains the actual position of the reference point of the reference point according to the position deviation between the reference point and the center of the field of view; obtains the actual center position of the target microporous according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target microporous; moves the detection device until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target microporous. In this way, the deformation of the flexible microporous plate can be avoided from affecting the detection, thereby realizing accurate detection of the object to be detected in the target microporous area.

[0132] It should be noted that the logic and / or steps represented in the flowchart or otherwise described herein can be considered as a sequenced list of executable instructions for implementing logical functions, and can be specifically implemented in any computer-readable medium for use by an instruction execution system, device or apparatus (such as a computer-based system, a system including a processor, or other system that can fetch instructions from an instruction execution system, device or apparatus and execute instructions), or in combination with these instruction execution systems, devices or apparatuses. For the purposes of this specification, "computer-readable medium" can be any device that can contain, store, communicate, propagate or transmit a program for use by an instruction execution system, device or apparatus, or in combination with these instruction execution systems, devices or apparatuses. More specific examples of computer-readable media (a non-exhaustive list) include the following: an electrical connection portion with one or more wirings (electronic device), a portable computer disk box (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable and programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disk read-only memory (CDROM). In addition, the computer-readable medium may even be paper or other suitable medium on which the program is printed, since the program may be obtained electronically, for example, by optically scanning the paper or other medium and then editing, interpreting or processing in other suitable ways if necessary, and then stored in a computer memory.

[0133] It should be understood that the various parts of the present invention can be implemented by hardware, software, firmware or a combination thereof. In the above-mentioned embodiment, a plurality of steps or methods can be implemented by software or firmware stored in a memory and executed by a suitable instruction execution system. If implemented by hardware, as in another embodiment, it can be implemented by any one of the following technologies known in the art or their combination: a discrete logic circuit having a logic gate circuit for implementing a logic function for a data signal, a dedicated integrated circuit having a suitable combination of logic gate circuits, a programmable gate array (PGA), a field programmable gate array (FPGA), etc.

[0134] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0135] In the description of this specification, the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and cannot be understood as a limitation on the present invention.

[0136] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0137] In the description of this specification, unless otherwise specified, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0138] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0139] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A detection method based on a flexible microplate, characterized in that: A thin film is provided at the bottom of the flexible microporous plate, and the method comprises: Determining a target microwell on the flexible microwell plate, and determining at least one reference point on the target microwell; Moving the detection device to move the center of the field of view of the detection device to a theoretical reference point position of the reference point; Obtaining the actual position of the reference point of the reference point according to the position deviation between the reference point and the center of the field of view; Obtaining the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore; The detection device is moved until the center of the field of view is located at the actual center position, so as to detect the object to be detected in the target film area, wherein the target film area is the film area corresponding to the target micropore.

2. The detection method based on the flexible microplate according to claim 1, characterized in that: The obtaining the actual center position of the target micropore according to the theoretical position of the reference point, the actual position of the reference point and the theoretical center position of the target micropore comprises: For each of the reference points, the theoretical coordinates of the theoretical position of the reference point in a preset coordinate system and the actual coordinates of the actual position of the reference point in the preset coordinate system are obtained, and the coordinate difference between the theoretical coordinates and the actual coordinates is obtained; Obtaining a coordinate correction value according to the coordinate difference, the resolution of the detection device and the field of view size; The theoretical center coordinates of the theoretical center position in the preset coordinate system are obtained, and the theoretical center coordinates are corrected according to the coordinate correction value, and the actual center position is obtained according to the corrected theoretical center coordinates.

3. The detection method based on the flexible microplate according to claim 2, characterized in that: The field of view of the detection device is a square field of view with a side length of a first length, the reference point includes a first reference point, the coordinate difference includes a first x-axis direction coordinate difference and a first y-axis direction coordinate difference between theoretical coordinates and actual coordinates corresponding to the first reference point in the preset coordinate system, and the coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: Obtaining a first product of the first x-axis direction coordinate difference and the resolution, and obtaining a second product of the first y-axis direction coordinate difference and the resolution; The first product is divided by the first length to obtain a coordinate correction value in the x-axis direction, and the second product is divided by the first length to obtain a coordinate correction value in the y-axis direction.

4. The detection method based on the flexible microplate according to claim 2, characterized in that: The field of view of the detection device is a square field of view with a side length of a second length, the reference points include a second reference point and a third reference point, the coordinate difference includes a second x-axis coordinate difference and a second y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the second reference point in the preset coordinate system, and also includes a third x-axis coordinate difference and a third y-axis coordinate difference between the theoretical coordinates and the actual coordinates corresponding to the third reference point in the preset coordinate system, wherein a line segment with the second reference point and the third reference point as endpoints passes through the micropore center of the target micropore, and the coordinate correction value is obtained according to the coordinate difference, the resolution of the detection device and the field of view size, including: When the first distance between the second reference point and the center of the microhole is equal to the second distance between the third reference point and the center of the microhole, the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the second length and the resolution; When the first distance is not equal to the second distance, obtain the fourth x-axis coordinate difference and the fourth y-axis coordinate difference between the second reference point and the center of the microhole in the preset coordinate system, and obtain the fifth x-axis coordinate difference and the fifth y-axis coordinate difference between the third reference point and the center of the microhole in the preset coordinate system, and obtain the coordinate correction value according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fourth y-axis coordinate difference, the fifth x-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

5. The detection method based on the flexible microplate according to claim 4, characterized in that: The coordinate correction value includes an x-axis direction coordinate correction value and a y-axis direction coordinate correction value, and the coordinate correction value is obtained according to the second x-axis direction coordinate difference value, the second y-axis direction coordinate difference value, the third x-axis direction coordinate difference value, the third y-axis direction coordinate difference value, the second length and the resolution, including: Obtaining a first mean value of the second x-axis direction coordinate difference and the third x-axis direction coordinate difference, and obtaining a second mean value of the second y-axis direction coordinate difference and the third y-axis direction coordinate difference; Multiplying the first average by the second length, and dividing the multiplication result by the resolution to obtain the x-axis direction coordinate correction value; The second mean value is multiplied by the second length, and the multiplication result is divided by the resolution to obtain the coordinate correction value in the y-axis direction.

6. The detection method based on the flexible microplate according to claim 4, characterized in that: The coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fourth y-axis coordinate difference, the fifth x-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution, including: When the fourth y-axis coordinate difference and the fifth y-axis coordinate difference are both zero, the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth x-axis coordinate difference, the fifth x-axis coordinate difference, the second length and the resolution; When the fourth x-axis coordinate difference and the fifth x-axis coordinate difference are both zero, the coordinate correction value is obtained according to the second x-axis coordinate difference, the second y-axis coordinate difference, the third x-axis coordinate difference, the third y-axis coordinate difference, the fourth y-axis coordinate difference, the fifth y-axis coordinate difference, the second length and the resolution.

7. The detection method based on the flexible microplate according to claim 1, characterized in that: The moving detection device to move the field of view center of the detection device to the reference point theoretical position of the reference point includes: Moving the detection device according to the theoretical center position of the target micropore to move the center of the field of view to the theoretical center position; The moving path is determined according to the size of the target micropore, and the detection device is moved according to the moving path so that the center of the visual field after the movement is located at the theoretical position of the reference point.

8. The detection method based on the flexible microplate according to claim 1, characterized in that: The moving detection device to move the field of view center of the detection device to the reference point theoretical position of the reference point includes: Determining the theoretical position of the reference point according to the theoretical center position and size of the target micropore; The detection device is moved according to the theoretical position of the reference point so that the center of the visual field after the movement is located at the theoretical position of the reference point.

9. An electronic device, characterized in that: The method comprises a memory, a processor and a computer program stored in the memory and running on the processor. When the computer program is executed by the processor, the flexible microplate-based detection method as described in any one of claims 1 to 8 is implemented.

10. A detection system based on a flexible microplate, characterized in that: It comprises a flexible microporous plate, a detection device, a moving device and a controller, wherein a thin film is provided at the bottom of the flexible microporous plate, and the controller comprises the electronic device according to claim 9; Among them, the moving device is connected to the detection device and the controller to move the detection device under the control of the controller, and the detection device is connected to the controller to detect the object to be detected in the target film area under the control of the controller. The target film area is the film area corresponding to the preset target micropores on the flexible micropore plate.

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