Micron particle monodispersion device

Abstract

The invention discloses a micron particle monodispersion device which is characterized in that a focusing flow channel is internally provided with a chamber and an ejection hole, and the ejection hole is communicated with the chamber and the external environment; the inner diameter of a core capillary tube of the liquid flow introduction module is far larger than the particle size of the micron particles, the outlet end of the core capillary tube extends into the cavity and is close to the ejection hole in an opposite mode, and the core capillary tube is used for conveying micron particle suspension liquid; the airflow introduction module is connected with the focusing flow channel and used for introducing high-pressure gas into the cavity, so that pressure difference is formed between the cavity and the external environment, and then micron particle suspension flowing out of the outlet end of the core capillary tube is pulled into superfine liquid jet flow under the wrapping and extruding action of coaxial sheath gas flow at the ejection hole; and the liquid drops are automatically crushed into liquid drops of monodisperse particles outside the focused flow channel. The device is not easy to block, high in micron particle monodispersion efficiency and high in flux, and can be compatible with an organic mass spectrum, a spectrum analyzer and the like.

Description

Technical Field

[0001] The invention relates to the technical field of micron particle monodispersion, and in particular to a micron particle monodispersion device. Background Art

[0002] At present, there are several types of micron particle monodisperse technologies:

[0003] One is to use a capillary tube with an inner diameter close to the diameter of micron particles such as cells to monodisperse micron particles such as cells. Since the inner diameter of the capillary tube is close to the diameter of the micron particles, only one particle is allowed to enter the capillary tube at a time under the action of air pressure, thus achieving monodisperse micron particles such as cells. The outlet section of the capillary tube is etched with hydrofluoric acid to obtain an outlet tip with a constant inner diameter and a reduced outer diameter, which avoids the accumulation of cells after dispersion and is also conducive to the final emission of cells into analytical detectors such as mass spectrometry. Since this method uses a liquid flow close to the diameter of the cell to introduce the flow channel, it is very easy to get clogged.

[0004] The second is to use Dean flow to monodisperse micron particles such as cells, which requires several thousand volts of high voltage to assist particle emission. Higher voltages are not compatible with some ionization methods. High voltage is usually loaded on a tee that serves as a connection. Long-term operation will cause cell debris to accumulate in the dead volume, thereby clogging the pipeline.

[0005] The third method is to use flow focusing to monodisperse micron particles such as cells, use a sheath flow with a higher flow rate to focus the cell suspension introduced by the core flow, and use a high voltage of several thousand volts to assist the cell emission. Since this method requires a high flow rate of sheath flow to focus and disperse micron particles such as cells, it is not suitable for detection methods using gas mass spectrometry or liquid chromatography as detectors. Summary of the invention

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, one object of the present invention is to provide a micron particle monodispersion device that is not easy to clog, has high micron particle monodispersion efficiency, high throughput, and is compatible with organic mass spectrometers and spectrum analyzers.

[0007] The micron particle monodisperse device according to an embodiment of the present invention comprises:

[0008] A focusing flow channel, wherein a chamber and an ejection hole are provided in the focusing flow channel, and the ejection hole communicates with the chamber and an external environment;

[0009] A liquid flow introduction module, the liquid flow introduction module comprises a core capillary tube, the inner diameter of the core capillary tube is much larger than the particle diameter of the micron particles, the outlet end of the core capillary tube extends into the chamber and is directly adjacent to the ejection hole, and the core capillary tube is used to transport the micron particle suspension;

[0010] An airflow introduction module is connected to the focusing flow channel and is used to introduce high-pressure gas into the chamber to form a pressure difference between the chamber and the external environment, so that the micron particle suspension flowing out of the outlet end of the core capillary is pulled into an extremely fine liquid jet under the wrapping and squeezing action of the coaxial sheath gas flow at the ejection hole, and is autonomously broken into droplets of monodisperse particles outside the focusing flow channel.

[0011] According to the micron particle monodisperse device of the embodiment of the present invention, on the one hand, since the inner diameter of the core capillary is much larger than the particle size of the micron particles, the micron particle suspension flows from the input end of the core capillary to the output end of the core capillary, which can effectively avoid the clogging of the core capillary; on the other hand, the sheath gas drives the liquid flow of the micron particle suspension to form an extremely fine jet and autonomously breaks into droplets of monodisperse micron particles. Specifically, high-pressure gas is continuously and stably injected into the chamber through the airflow introduction module to form a pressure difference between the chamber and the external environment, and then the high-pressure gas in the chamber forms a focused sheath gas airflow near the ejection hole, and the sheath gas wraps the liquid flow of the micron particle suspension flowing out of the outlet end of the core capillary, and the sheath gas is used to focus and squeeze the liquid flow of the micron particle suspension, and the pressure difference inside and outside the chamber and the liquid flow rate of the core capillary are appropriately controlled. Under the conditions, the diameter of the ultrafine jet can reach a size close to that of the micron particles, so that the micron particles can be arranged in a row to form an ultrafine jet. After the ultrafine jet breaks up autonomously, monodisperse micron particles wrapped by droplets are formed. Since the frequency of the ultrafine jet focused to break up into droplets with monodisperse micron particles is extremely high, between KHz-MHz, the efficiency is relatively high, so a higher flux can be achieved. This avoids the problem of high sheath flow rate required to achieve high throughput in the flow focusing scheme of the prior art, and does not require high-voltage electrospray to assist liquid flow emission. Therefore, under high-throughput conditions, it can be compatible with organic mass spectrometers and spectral analyzers, that is, after the ultrafine jet breaks up autonomously, monodisperse micron particles wrapped by droplets are formed, which can directly enter the analyzer, and the analyzer includes but is not limited to organic mass spectrometers and spectrometers. In summary, the micron particle monodisperse device of the embodiment of the present invention is not easy to be clogged and does not require complex liquid flow pipelines to form special liquid flow power to disperse the particles in the liquid flow. Instead, it uses an extremely fine jet with a size close to the particle diameter and an extremely fine jet to break into droplets of monodisperse micron particles. The micron particle monodisperse efficiency is high, the throughput is high, and it is compatible with organic mass spectrometers and spectrometers, etc.

[0012] In some embodiments, the focusing flow channel includes a chamber body and an orifice plate; the orifice plate is provided with the ejection hole, the orifice plate is fixed on the chamber body and forms the chamber between the orifice plate and the chamber body; the core capillary passes through the chamber body so that the outlet end of the core capillary extends into the chamber; the airflow introduction module is connected to the chamber body.

[0013] In some embodiments, the chamber body includes a base and two protrusions located alternately on the same side of the base, and the orifice plate is opposite to the base and fixed on the two protrusions; the focusing flow channel also includes two side plates, and the two side plates are correspondingly distributed on opposite sides of the chamber body and fixed to the chamber body and the orifice plate, so that the base, the two protrusions, the orifice plate and the two side plates together form the chamber.

[0014] In some embodiments, the side panels are transparent panels.

[0015] In some embodiments, the focusing flow channel further comprises a support assembly for supporting the tube section of the core capillary located in the chamber.

[0016] In some embodiments, the support assembly includes N support plates, and the N support plates are fixed between the two protrusions and the orifice plate by spacers; the support plates are provided with limiting support holes for the core capillary to pass through, and the tube section of the core capillary located in the chamber fits through the N support plates; and a plurality of exhaust holes are also evenly distributed on the support plates.

[0017] In some embodiments, the liquid flow introduction module also includes a sheath tube, a tee piece and a sheath flow injection pump; the sheath tube is coaxially sleeved outside the core capillary, the outlet end of the sheath tube extends into the chamber and is close to the outlet end of the core capillary, and the outlet end of the core capillary protrudes from the outlet end of the sheath tube; the tee piece fixes the sheath tube and the core capillary; the sheath flow injection pump is connected to the tee piece, and is used to inject sheath fluid into the sheath tube.

[0018] In some embodiments, the distance between the outlet end of the core capillary and the outlet end of the sheath tube is 50-500 um.

[0019] In some embodiments, the distance from the outlet end of the core capillary to the ejection hole is 0-500 um.

[0020] In some embodiments, the air flow introduction module includes a high-pressure gas source, an air inlet pipe and a gas flow controller; the high-pressure gas source is connected to the inlet end of the gas flow controller, the outlet end of the gas flow controller is connected to the inlet end of the air inlet pipe, and the outlet end of the air inlet pipe is connected to the chamber.

[0021] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0023] Figure 1 A schematic diagram of a micron particle monodispersion device according to an embodiment of the present invention;

[0024] Figure 2 for Figure 1 The enlarged schematic diagram at A in the middle;

[0025] Figure 3a A front view of a chamber body of a focusing flow channel of a micron particle monodisperse device according to an embodiment of the present invention;

[0026] Figure 3b for Figure 3a The cross-sectional view at BB;

[0027] Figure 3c A side view of a chamber body of a focusing flow channel of a micron particle monodisperse device according to an embodiment of the present invention;

[0028] Figure 4 A front view of an orifice plate of a focusing flow channel of a micron particle monodisperse device according to an embodiment of the present invention;

[0029] Figure 5 A front view of a support plate of a focusing flow channel of a micron particle monodisperse device according to an embodiment of the present invention;

[0030] Figure 6a A front view of a spacer block of a focusing flow channel of a micron particle monodispersion device according to an embodiment of the present invention;

[0031] Figure 6b for Figure 6a Cross-sectional view at CC of ;

[0032] Figure 6c A side view of a spacer block of a focusing flow channel of a micron particle monodispersion device according to an embodiment of the present invention;

[0033] Figure 7a Schematic diagram showing the flow shape of a microparticle suspension at the outlet end of a core capillary;

[0034] Figure 7b Schematic diagram showing the process of a very fine jet outside the orifice of the orifice plate and its breakup into single droplets;

[0035] Figure 7c A schematic diagram showing monodisperse micron particles encapsulated in droplets captured by a microscope after the ultrafine jet breaks up autonomously is shown;

[0036] Figure 8aSchematic diagram showing the states of microspheres and cells in a mixed suspension of microspheres and cells that are not monodispersed;

[0037] Figure 8b A schematic diagram showing the state of the collected microspheres and cells after passing through the example device.

[0038] Reference numerals:

[0039] Micron particle monodisperse device 1000; focusing flow channel 1; chamber 101; chamber body 102; base 1021; convex portion 1022; tube hole 1023; air inlet hole 1024 orifice plate 103; ejection hole 1031; side plate 104; support plate 105; limit support hole 1051; exhaust hole 1052; cushion block 106; positioning column 107; threaded member 108; liquid flow introduction module 2; core capillary 201; sample tank 202; sample chamber 2021; injection gas pump 203; sheath tube 204; three-way piece 205; sheath flow injection pump 206; gas flow introduction module 3; high-pressure gas source 301; gas flow controller 302; inlet pipe 303. DETAILED DESCRIPTION

[0040] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0041] Combine the following Figures 1 to 8b The micron particle monodisperse device 1000 according to an embodiment of the present invention is described.

[0042] like Figures 1 to 8b As shown, the micron particle monodispersion device 1000 of an embodiment of the present invention is used to perform micron particle monodispersion on micron particle suspensions such as cells or microplastics, and includes a focusing flow channel 1, a liquid flow introduction module 2 and an air flow introduction module 3.

[0043] Among them, Figure 1 and Figure 2 As shown, a chamber 101 and an ejection hole 1031 are provided in the focusing flow channel 1, and the ejection hole 1031 communicates the chamber 101 with the external environment.

[0044] The liquid flow introduction module 2 includes a core capillary 201, the inner diameter of the core capillary 201 is much larger than the particle size of the micron particles, the outlet end of the core capillary 201 extends into the chamber 101 and is directly adjacent to the ejection hole 1031, and the core capillary 201 is used to transport the micron particle suspension. Since the inner diameter of the core capillary 201 is much larger than the particle size of the micron particles, the micron particle suspension flows from the input end of the core capillary 201 to the output end of the core capillary 201 without blocking the core capillary 201; since the outlet end of the core capillary 201 extends into the chamber 101 and is directly adjacent to the ejection hole 1031, it is beneficial for the micron particle suspension flowing out of the outlet end of the core capillary 201 to be ejected from the ejection hole 1031.

[0045] The air flow introduction module 3 is connected to the focusing flow channel 1 and is used to introduce high-pressure gas into the chamber 101, so that a pressure difference is formed between the chamber 101 and the external environment, thereby causing the micron particle suspension flowing out of the outlet end of the core capillary 201 to flow in the coaxial sheath gas flow at the ejection hole 1031 (such as Figure 2 The micron particle suspension liquid flow flowing out of the core capillary 201 is simultaneously ejected out of the ejection hole 1031. In this process, the micron particle suspension liquid flow is focused and squeezed by the sheath gas. Under the conditions of the appropriate pressure difference between the inside and outside of the chamber 101 and the liquid flow rate of the core capillary 201, the diameter of the stable ultrafine jet can reach a size close to that of the micron particle, so that the micron particle can be arranged in a row. After the ultrafine jet is autonomously broken, the monodisperse micron particle wrapped by the droplets is formed and enters the analyzer, which includes but is not limited to an organic mass spectrometer or a spectrometer.

[0046] According to the micron particle monodisperse device 1000 of the embodiment of the present invention, on the one hand, since the inner diameter of the core capillary 201 is much larger than the particle size of the micron particles, the micron particle suspension flows from the input end of the core capillary 201 to the output end of the core capillary 201, which can effectively avoid the clogging of the core capillary 201; on the other hand, the sheath gas drives the micron particle suspension flow to form an extremely fine jet and autonomously breaks into droplets of monodisperse micron particles (such as Figure 2As shown), specifically, high-pressure gas is continuously and stably injected into the chamber 101 through the airflow introduction module 3, so that a pressure difference is formed between the chamber 101 and the external environment, and then the high-pressure gas in the chamber 101 forms a focused sheath gas flow near the ejection hole 1031, and the sheath gas wraps the micron particle suspension liquid flow flowing out from the outlet end of the core capillary 201, and the sheath gas is used to focus and squeeze the micron particle suspension liquid flow. Under the conditions of the appropriate pressure difference between the inside and outside of the focusing flow chamber 101 and the liquid flow rate of the core capillary 201, the diameter of the ultra-fine jet can reach a size close to that of the micron particles, so that the micron particles can be arranged in a row to form an ultra-fine jet. After autonomous shattering, monodisperse micron particles wrapped by droplets are formed. Since the frequency of the ultrafine jets that are focused to break into droplets with monodisperse micron particles is extremely high, between 100KHz-1MHz, the efficiency is relatively high, so a higher flux can be achieved, which avoids the problem of high sheath flow rate required to achieve high flux in the flow focusing scheme of the prior art, and does not require high-voltage electrospray to assist the liquid flow outflow, so that it can be compatible with organic mass spectrometers and spectrometers under high-throughput conditions, that is, after the ultrafine jets are autonomously broken, monodisperse micron particles wrapped by droplets are formed, which can directly enter the analyzer, which includes but is not limited to organic mass spectrometers and spectrometers. In short, the micron particle monodisperse device 1000 of the embodiment of the present invention is not easy to be blocked, does not require complex liquid flow pipelines to form special liquid flow power to disperse the particles in the liquid flow, but uses ultrafine jets with a size close to the particle diameter and ultrafine jets to break into droplets of monodisperse micron particles. The micron particle monodisperse efficiency is high, the flux is high, and it is compatible with organic mass spectrometers and spectrometers.

[0047] In some embodiments, Figures 1 to 6c As shown, the focusing flow channel 1 includes a chamber body 102 and an orifice plate 103; the orifice plate 103 is provided with an ejection hole 1031, the orifice plate 103 is fixed on the chamber body 102 and forms a chamber 101 with the chamber body 102; the core capillary 201 passes through the chamber body 102, so that the outlet end of the core capillary 201 extends into the chamber 101, and in detail, the chamber body 102 is provided with a tube hole 1023 for the core capillary 201 to pass through, and the core capillary 201 The outlet end of the core capillary 201 extends into the chamber 101 through the tube hole 1023 and can be close to the ejection hole 1031 of the orifice plate 103; the air flow introduction module 3 is connected to the chamber body 102. Specifically, the chamber body 102 is provided with an air inlet hole 1024 connected to the air flow introduction module 3. The air flow introduction module 3 is connected to the air inlet hole 1024, and then communicates with the chamber 101 through the air inlet hole 1024 to input high-pressure gas into the chamber 101.

[0048] In some embodiments, the chamber body 102 includes a base 1021 and two convex portions 1022 located on the same side of the base 1021 and spaced from each other, and the orifice plate 103 is opposite to the base 1021 and fixed on the two convex portions 1022; the focusing flow channel 1 also includes two side plates 104, which are correspondingly distributed on opposite sides of the chamber body 102 and fixed to the chamber body 102 and the orifice plate 103, so that the base 1021, the two convex portions 1022, the orifice plate 103 and the two side plates 104 together enclose the chamber 101. Therefore, the focusing flow channel 1 has a simple structure and is easy to process and assemble.

[0049] In some embodiments, the side panel 104 is a transparent panel for easy observation. Preferably, the transparent panel is ultra-clear glass, which has good transparency and is resistant to high pressure.

[0050] In some embodiments, the focusing flow channel 1 further includes a support assembly, which is used to support the tube section of the core capillary 201 located in the chamber 101 to stabilize the output end of the core capillary 201, which is conducive to forming a stable ultra-fine jet.

[0051] In some embodiments, the support assembly includes N support plates 105 (N is an integer greater than or equal to 1), and the N support plates 105 are fixed between the two protrusions 1022 and the orifice plate 103 at intervals through the spacers 106. For example, Figure 1 Two support plates 105 are shown in the figure, wherein the two opposite ends of one support plate 105 abut against the two protrusions 1022, and the other support plate 105 is separated from the other support plate 105 and the orifice plate 103 by a spacer 106; the support plate 105 is provided with a limiting support hole 1051 (such as Figure 5 As shown), the tube section of the core capillary 201 located in the chamber 101 fits through N support plates 105, which can stabilize the output end of the core capillary 201 and is conducive to forming a stable ultra-fine jet; a plurality of exhaust holes 1052 are evenly distributed on the support plate 105, which is conducive to the high-pressure airflow to pass through the chamber 101 evenly and flow to the ejection hole 1031.

[0052] In some embodiments, the orifice plate 103, the support plate 105, the cushion block 106 and the protrusion 1022 are positioned by the positioning column 107 and fastened by the screw member 108. Figure 1As shown, positioning holes are provided on the orifice plate 103, the support plate 105, the pad 106 and the convex portion 1022. The positioning column 107 passes through the positioning holes on the orifice plate 103, the support plate 105, the pad 106 and the convex portion 1022 to realize the positioning between the orifice plate 103, the support plate 105, the pad 106 and the convex portion 1022. On the one hand, it is convenient for the threaded fasteners to be fixed in the corresponding fixing holes on the orifice plate 103, the support plate 105, the pad 106 and the convex portion 1022. On the other hand, it is beneficial for the output end of the core capillary 201 to be coaxially opposite to the injection hole 1031 of the orifice plate 103.

[0053] In some embodiments, Figure 1 As shown, the liquid flow introduction module 2 also includes a sample tank 202 and a sampling air pump 203; the sample tank 202 is made of metal, plastic or glass, and has a sample chamber 2021 for containing a micron particle suspension, the inlet end of the core capillary 201 extends into the sample chamber 2021, and the sampling air pump 203 is connected to the sample chamber 2021. When working, the sampling air pump 203 injects air above the liquid surface of the micron particle suspension in the sample chamber 2021, so that the micron particle suspension enters the core capillary 201 from the input end of the core capillary 201, and then flows out from the core capillary 201.

[0054] In some embodiments, Figure 1 As shown, the liquid flow introduction module 2 also includes a sheath tube 204, a tee 205 and a sheath flow injection pump 206; the sheath tube 204 is coaxially sleeved outside the core capillary 201, the outlet end of the sheath tube 204 extends into the chamber 101 and is close to the outlet end of the core capillary 201, and the outlet end of the core capillary 201 protrudes from the outlet end of the sheath tube 204, which is conducive to forming a stable jet at a lower liquid flow rate; the tee 205 fixes the sheath tube 204 and the core capillary 201, and the fixation is convenient and reliable; the sheath flow injection pump 206 is connected to the tee 205, and is used to inject sheath liquid into the sheath tube 204, that is, to inject sheath liquid into the annular liquid flow channel of the sheath tube 204 and the core capillary 201, and the sheath liquid flowing out from the output end of the sheath tube 204 can coaxially wrap the micron particle suspension, which is conducive to improving the ionization effect of the monodisperse micron particles broken into extremely fine liquid flows. When the ionization effect of monodisperse micron particles is not required, the sheath tube 204, the tee piece 205 and the sheath flow injection pump 206 may not be provided. The sheath tube 204 and the core capillary 201 are made of metal, plastic or glass such as quartz glass.

[0055] In some embodiments, the distance between the outlet end of the core capillary 201 and the outlet end of the sheath tube 204 is 50-500 um, which is conducive to forming a stable jet at a lower liquid flow rate.

[0056] In some embodiments, the distance from the outlet end of the core capillary 201 to the ejection hole 1031 is 0-500um. Since the core working principle of the embodiment of the present invention is to use the air pressure distribution near the airflow ejection hole 1031 to squeeze the droplet cone, and finally form a very fine jet at the front end of the droplet cone, appropriately adjusting the distance from the outlet end of the core capillary 201 to the ejection hole 1031 is conducive to forming a stable jet under different liquid flow rates.

[0057] In some embodiments, the inner diameter of the core capillary 201 is 25-500 um, and the core capillary 201 is not easily blocked.

[0058] In some embodiments, Figure 1 As shown, the air flow introduction module 3 includes a high-pressure gas source 301, a gas flow controller 302 and an air inlet pipe 303; the high-pressure gas source 301 can be a high-pressure gas cylinder or an air compressor, etc. The high-pressure gas source 301 is connected to the inlet end of the gas flow controller 302, the outlet end of the gas flow controller 302 is connected to the inlet end of the air inlet pipe 303, and the outlet end of the air inlet pipe 303 is connected to the chamber 101. Among them, the gas flow controller 302 can be controlled by volume flow, mass flow or pipeline pressure, and no matter which control method is used, the volume flow, mass flow or pipeline pressure can be monitored simultaneously.

[0059] The following is an example of monodispersing micron particles using a micron particle suspension. In the micron particle suspension sample, the cells are MCF7, the diameter of the polystyrene microspheres is 10um, the cells require the solution to be an isotonic solution, and the polystyrene microspheres have no requirements for the properties of the solution. Therefore, in accordance with the requirements of the cells, the cells and the polystyrene microspheres are dissolved in an isotonic ammonium formate solution to form a mixed suspension, i.e., a micron particle suspension. The liquid flow path adopts a double-layer structure of a core capillary 201 and a sheath tube 204. By adjusting the appropriate internal and external pressure difference of the focusing flow channel 1 and the liquid flow sample supply, the sheath gas flow rate is 190ml / min, the internal and external pressure difference is 7.3kPa, the liquid flow rate of the core capillary 201 is 2ul / min, and the liquid flow rate of the sheath tube 204 is 20ul / min, the diameter of the ultrafine jet can be about 10um, so the ultrafine jet and its autonomous breakup into a single droplet process can achieve monodisperse micron particles. Figure 7a The flow shape of the micron particle suspension at the outlet end of the core capillary 201 is shown; Figure 7b The figure shows the process of the extremely fine jet outside the ejection hole 1031 and its breakup into single droplets. Figure 7c A schematic diagram showing monodisperse micron particles encapsulated in droplets captured under a microscope after an extremely fine jet breaks up autonomously. Figure 8a The states of microspheres and cells in a mixed suspension of microspheres and cells without monodispersity are shown; Figure 8bThe state of the microspheres and cells collected after passing through the example device is shown. It can be seen that the microspheres and cells are effectively monodispersed, and if connected to an analyzer, single-cell online measurement can be further achieved.

[0060] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0061] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A micron particle monodisperse device, characterized in that: include: A focusing flow channel, wherein a chamber and an ejection hole are provided in the focusing flow channel, and the ejection hole communicates with the chamber and an external environment; A liquid flow introduction module, the liquid flow introduction module comprises a core capillary tube, the inner diameter of the core capillary tube is much larger than the particle diameter of the micron particles, the outlet end of the core capillary tube extends into the chamber and is directly adjacent to the ejection hole, and the core capillary tube is used to transport the micron particle suspension; An airflow introduction module is connected to the focusing flow channel and is used to introduce high-pressure gas into the chamber to form a pressure difference between the chamber and the external environment, so that the micron particle suspension flowing out of the outlet end of the core capillary is pulled into an extremely fine liquid jet under the wrapping and squeezing action of the coaxial sheath gas flow at the ejection hole, and is autonomously broken into droplets of monodisperse particles outside the focusing flow channel.

2. The micron particle monodisperse device according to claim 1, characterized in that: The focusing flow channel includes a chamber body and an orifice plate; the orifice plate is provided with the ejection hole, the orifice plate is fixed on the chamber body and forms the chamber with the chamber body; the core capillary passes through the chamber body so that the outlet end of the core capillary extends into the chamber; the airflow introduction module is connected to the chamber body.

3. The micron particle monodisperse device according to claim 2, characterized in that: The chamber body includes a base and two convex portions located alternately on the same side of the base, and the orifice plate is opposite to the base and fixed on the two convex portions; the focusing flow channel also includes two side plates, which are correspondingly distributed on opposite sides of the chamber body and fixed to the chamber body and the orifice plate, so that the base, the two convex portions, the orifice plate and the two side plates together form the chamber.

4. The micron particle monodisperse device according to claim 3, characterized in that: The side plate is a transparent plate.

5. The micron particle monodisperse device according to claim 3, characterized in that: The focusing flow channel further includes a supporting assembly, and the supporting assembly is used to support the tube section of the core capillary located in the chamber.

6. The micron particle monodisperse device according to claim 5, characterized in that: The support assembly comprises N support plates, and the N support plates are fixed between the two protrusions and the orifice plate at intervals through spacers; The support plate is provided with a limiting support hole for the core capillary to pass through, and the tube section of the core capillary located in the chamber fits through the N support plates; and a plurality of exhaust holes are evenly distributed on the support plate.

7. The micron particle monodisperse device according to any one of claims 1 to 6, characterized in that: The liquid flow introduction module further comprises a sheath tube, a tee piece and a sheath flow injection pump; the sheath tube is coaxially sleeved outside the core capillary tube, the outlet end of the sheath tube extends into the chamber and is close to the outlet end of the core capillary tube, and the outlet end of the core capillary tube protrudes from the outlet end of the sheath tube; The three-way piece fixes the sheath tube and the core capillary; the sheath flow injection pump is connected to the three-way piece and is used for injecting sheath fluid into the sheath tube.

8. The micron particle monodisperse device according to claim 7, characterized in that: The distance between the outlet end of the core capillary and the outlet end of the sheath tube is 50-500 um.

9. The micron particle monodisperse device according to any one of claims 1 to 6, characterized in that: The distance from the outlet end of the core capillary to the ejection hole is 0-500um.

10. The micron particle monodisperse device according to any one of claims 1 to 6, characterized in that: The air flow introduction module includes a high-pressure gas source, an air intake pipe and a gas flow controller; the high-pressure gas source is connected to the inlet end of the gas flow controller, the outlet end of the gas flow controller is connected to the inlet end of the air intake pipe, and the outlet end of the air intake pipe is connected to the chamber.

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