A rapid sampling and detection device for pine wood nematode disease

Abstract

The present invention relates to the technical field of plant protection and forestry disease prevention and control, and in particular to a rapid sampling and detection device for pine wood nematode disease, comprising: a collecting mechanism, the collecting mechanism comprising a base frame, a liquid collecting frame, a supporting threaded rod, a carrier, a first separation component and a linkage component, the liquid collecting frame is fixedly connected to the inner bottom surface of the base frame, the supporting threaded rod is rotatably connected to the inner bottom surface of the base frame, and the carrier is threadedly connected to the outer surface of the supporting threaded rod. In the present invention, when in use, the existing filter is placed through the placement groove, and the support motor is controlled to start, so that the support motor cooperates with the support worm and the support worm gear to drive the support threaded rod to rotate, and then under the position limit of the guide rod, the rotating support threaded rod can increase the use height of the carrier, and then the processed sample wood is placed on the top of the partition frame, and then the limit motor is controlled to start, so that the limit motor cooperates with the limit worm and the limit worm gear to drive the bidirectional threaded rod to rotate.

Description

Technical Field

[0001] The invention belongs to the technical field of plant protection and forestry disease prevention and control, and specifically relates to a rapid sampling and detection device for pine wood nematode disease. Background Art

[0002] Pine wilt disease is a devastating forest disease caused by the pine wood nematode. It is a major invasive alien species in China and has been listed as an object of internal and external forest plant quarantine by China. Since the disease was introduced into China in 1982, it has spread rapidly, causing the death of a large number of pine trees, causing serious damage to China's pine forest resources, natural landscape and ecological environment, and causing serious economic and ecological losses.

[0003] However, in the prior art, pine wood nematode sampling is mostly performed by soaking sample wood blocks for a long time and then filtering them to obtain the filtered solution, and then observing and monitoring the filtered solution to achieve sampling and detection of pine wood nematodes. The actual processing time is relatively long, resulting in poor efficiency of actual sampling and detection. Summary of the invention

[0004] The purpose of the present invention is to provide a pine wood nematode disease rapid sampling and detection device which can efficiently obtain pine wood nematodes in sampled wood blocks and improve actual detection efficiency.

[0005] The technical solution adopted by the present invention is as follows: a rapid sampling and detection device for pine wood nematode disease, comprising: a collecting mechanism, the collecting mechanism comprising a base frame, a liquid collecting frame, a supporting threaded rod, a carrier, a first separating component and a linkage component, the liquid collecting frame is fixedly connected to the inner bottom surface of the base frame, the supporting threaded rod is rotatably connected to the inner bottom surface of the base frame, the carrier is threadedly connected to the outer surface of the supporting threaded rod, the first separating component is arranged on the carrier, and the linkage component is arranged on the liquid collecting frame; and

[0006] The separation mechanism comprises a material guide frame, a card frame, a top frame, a resist frame, a resist block, a vehicle body, a rotating block, a saw blade, a limiting component, an adjusting component and a second separation component. The material guide frame is fixedly connected to the top of the bottom frame, the top frame is arranged on the top of the material guide frame, a moving port and a feeding port are opened on the top of the top frame, the card frame is connected and arranged on the bottom surface of the inner part of the material guide frame, the resist frame is fixedly connected to the outer surface of one side of the card frame, the resist block is slidably inserted in the moving port, and the bottom of the resist block is attached to the top of the resist frame, the vehicle body is placed on the top of the top frame, and the bottom of the vehicle body is fixedly connected to the top of the resist block, the rotating block is slidably sleeved on the outer surface of the resist block, the saw blade is sleeved on the outer surface of the rotating block, and the limiting component, the adjusting component and the second separation component are all arranged on the top frame.

[0007] Wherein, a guide rod is fixedly connected to the inner bottom surface of the base frame, and one end of the guide rod slides through the bottom of the carrier.

[0008] Among them, the outer surface of the supporting threaded rod is sleeved with a supporting worm wheel, the inner bottom surface of the base frame is rotatably connected with a supporting worm, the supporting worm and the supporting worm wheel are meshed, the inner bottom surface of the base frame is fixedly connected with a supporting motor, and the output end of the supporting motor is fixedly connected to one end of the supporting worm.

[0009] Wherein, an elastic sealing ring is sleeved on the outer surface of the carrier, a partition is placed on the top of the carrier, and a placement groove is opened on the top of the carrier.

[0010] Among them, the first separation component includes a first rotating frame, a first connecting frame and an insertion frame, the first rotating frame is slidably sleeved on the outer surface of the carrier, the outer surface of the first rotating frame is sleeved with a first linkage wheel, the inner surface wall of the first rotating frame is equidistantly fixedly connected with a plurality of paddles, the bottom of the first rotating frame is equidistantly connected with a plurality of drainage pipes, the bottom end of each drainage pipe is threadedly connected with a sealing cover, the outer surface of the first rotating frame is slidably sleeved with a liquid transfer frame, the bottom of the liquid transfer frame is fixedly connected to the inner bottom surface of the base frame, the inner bottom surface of the liquid transfer frame is penetrated by a liquid guide pipe, one end of the liquid guide pipe extends to the inside of the liquid collecting frame, the first connecting frame is rotatably connected to the outer surface of the first rotating frame, the first connecting frame is inserted into the inside of the card frame, the insertion frame is connected and arranged on the outer surface of one side of the first connecting frame, and one end of the insertion frame extends to the outside of the material guide frame.

[0011] Among them, the linkage component includes a linkage shaft and a linkage motor, the linkage shaft is rotatably connected to the top of the liquid collecting frame, the outer surface of the linkage shaft is sleeved with a second linkage wheel and a linkage gear, a first linkage belt is sleeved between the outer surface of the second linkage wheel and the outer surface of the first linkage wheel, the linkage motor is fixedly connected to the top of the liquid collecting frame, and the output end of the linkage motor is also sleeved with a linkage gear, and the two linkage gears are meshed.

[0012] The transmission gear of the present invention is connected with the gear of the transmission gear of the present invention to the gear of the transmission gear of the transmission gear of the transmission gear.

[0013] Among them, the limiting component includes a mounting frame, a two-way threaded rod, two clamping plates and a limiting motor, the mounting frame is fixedly connected to the top of the top frame, the two-way threaded rod is rotatably connected to the top of the mounting frame, the outer surface of the two-way threaded rod is sleeved with a limiting worm gear, the two clamping plates are threadedly connected to the outer surface of the two-way threaded rod, a reset frame is inserted into the outer surface of one side of each clamping plate, a clamping rod is slidably inserted inside each reset frame, a pressure ring is sleeved on the outer surface of each clamping rod, a limiting spring is slidably sleeved on the outer surface of each clamping rod, the limiting motor is fixedly connected to the top of the mounting frame, and a limiting worm is sleeved on the output end of the limiting motor, and the limiting worm is meshed with the limiting worm gear.

[0014] Among them, the second separation component includes a second connecting frame, a second rotating frame and a filter tube, the second connecting frame is connected and arranged at the bottom of the material guide frame, the second rotating frame is rotatably connected to the inside of the second connecting frame, the outer surface of the second rotating frame is sleeved with a third linkage wheel, a second linkage belt is sleeved between the outer surface of the third linkage wheel and the outer surface of the second linkage wheel, the filter tube passes through the bottom surface of the second rotating frame, and a sealing plug is inserted at the bottom end of the filter tube.

[0015] A method for using a rapid sampling and detection device for pine wood nematode disease, comprising the following steps:

[0016] S1. Preliminary extraction: Place the existing filter sheet through the placement slot, control and start the support motor, so that the support motor cooperates with the support worm and the support worm gear to drive the support threaded rod to rotate, and then under the position limit of the guide rod, the rotating support threaded rod can increase the use height of the carrier, and then place the processed sample wood on the top of the partition, and then control and start the limit motor, so that the limit motor cooperates with the limit worm and the limit worm gear to drive the two-way threaded rod to rotate, and then the rotating two-way threaded rod can drive the two clamps to approach each other, so that the two clamps can squeeze and fix the sample wood on the top of the partition through the clamp rod. The material is placed in a position, at this time, the moving motor is controlled to be started, so that the moving motor cooperates with the moving worm and the moving worm wheel to drive the threaded tube to rotate, and then the rotating threaded tube cooperates with the moving threaded rod to drive the vehicle body to move in the horizontal direction. At the same time, the rotating motor is controlled to be started, so that the rotating motor cooperates with the two rotating gears to drive the rotating block to rotate, and then the rotating rotating block can drive the saw blade to rotate. During the movement of the vehicle body, the vehicle body cooperates with the abutment block and the rotating block to drive the rotating saw blade to gradually approach the sample wood, so that the sample wood can be quickly split efficiently. At this time, the supporting motor and the moving motor are reversed to make the saw blade move. The pieces gradually return to their original positions, and the sample wood after the division at the bottom can be gradually lowered with the carrier until the top of the divided sample wood is lower than the bottom height of the insert frame, and another partition frame is erected to the top of the divided sample wood through the insert frame stacking. At the same time, under the position restriction of the card rod, the remaining part of the sample wood will maintain its original position. At this time, the support motor, the moving motor and the limit motor are controlled to start, so that the newly placed partition frame fits the bottom of the remaining sample wood again. At this time, the position restriction of the remaining sample wood on the card rod is released, so that the carrier can cooperate with the newly placed partition frame to carry the remaining sample wood and descend to a certain height, and then The saw blade is enabled to perform equidistant segmentation processing on the remaining sample wood, so that the sample wood can be segmented into multiple thinner wood chips. After the segmented sample wood is supported by the carrier, it is finally stored in the first rotating frame equidistantly with the partition frame in a cyclic process. At this time, an appropriate amount of a solution of a certain temperature with an attractant added is injected into the first rotating frame through the insertion frame and the first connecting frame, so that the pine wood nematodes remaining in the thinner sample wood can be quickly lured out of the sample wood. Then, by removing the sealing cover, the pine wood nematodes can be injected into the liquid transfer frame through the drainage pipe with the solution, and then transported to the liquid collection frame through the liquid guide pipe;

[0017] S2. Efficient extraction: During the process of splitting the sample wood, the generated debris is blocked by the material guide frame, the card frame and the insertion frame, so that the debris can fall into the second rotating frame. At this time, an appropriate amount of solution at a certain temperature with an attractant added is injected into the material guide frame, so that the solution can be injected into the second rotating frame through the material guide frame. At this time, the linkage motor is started by control, so that the linkage motor cooperates with the two linkage gears to drive the linkage shaft to rotate, and then the linkage shaft cooperates with the second linkage wheel, the first linkage belt and the second linkage belt to drive the first linkage wheel and the third linkage wheel to rotate, and then the first linkage wheel and the third linkage wheel can drive the first rotating frame and the second rotating frame to rotate, thereby accelerating the entry of pine wood nematodes in the split sample wood and debris into the solution, improving the actual sampling efficiency of pine wood nematodes of the equipment, and then under the filtration of the filter tube, the solution containing pine wood nematodes can be injected into the liquid collecting frame through the filter tube, and then the solution extraction and observation are carried out subsequently.

[0018] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0019] (1) In the present invention, when in use, the existing filter sheet is placed through the placement groove, and the support motor is controlled to be started, so that the support motor cooperates with the support worm and the support worm gear to drive the support threaded rod to rotate, and then under the position limit of the guide rod, the rotating support threaded rod can increase the use height of the carrier, and then the processed sample wood is placed on the top of the partition frame, and then the limit motor is controlled to be started, so that the limit motor cooperates with the limit worm and the limit worm gear to drive the bidirectional threaded rod to rotate, and then the rotating bidirectional threaded rod can drive the two clamps to approach each other, so that the two clamps that are close to each other can be squeezed and fixed by the clamp rod to place the sample wood on the top of the partition frame. At the same time, the moving motor is started by control, so that the moving motor cooperates with the moving worm and the moving worm wheel to drive the threaded tube to rotate, and then the rotating threaded tube cooperates with the moving threaded rod to drive the vehicle body to move in the horizontal direction. At the same time, the rotating motor is started by control, so that the rotating motor cooperates with the two rotating gears to drive the rotating block to rotate, and then the rotating rotating block can drive the saw blade to rotate. During the movement of the vehicle body, the vehicle body cooperates with the abutment block and the rotating block to drive the rotating saw blade to gradually approach the sample wood, so that the sample wood can be quickly split efficiently. At this time, the supporting motor and the moving motor are reversed to make the saw blade gradually return to its original position, and the sample split at the bottom The wood can be gradually lowered with the carrier until the top of the split sample wood is lower than the bottom height of the insert frame, and then another partition frame is erected to the top of the split sample wood through the insert frame stacking. At the same time, under the position limit of the clamping rod, the remaining part of the sample wood will maintain its original position. At this time, the support motor, the moving motor and the limit motor are controlled to start, so that the newly placed partition frame fits the bottom of the remaining sample wood again. At this time, the position limit of the remaining sample wood on the clamping rod is released, so that the carrier can cooperate with the newly placed partition frame to carry the remaining sample wood to a certain height, and then the saw blade can divide the remaining sample wood equidistantly, so that the sample wood can be The wood chips can be divided into multiple thinner pieces, which are processed in a cycle in sequence, so that the divided sample wood is supported by the carrier and finally stored in the first rotating frame at an equal distance from the partition frame. At this time, an appropriate amount of solution of a certain temperature added with an attractant is injected into the first rotating frame through the insertion frame and the first connecting frame, so that the pine wood nematodes remaining in the thinner sample wood can be quickly lured out of the sample wood, and then the sealing cover is removed so that the pine wood nematodes can follow the solution through the drainage pipe and injected into the transfer frame, and then transported to the liquid collecting frame through the liquid guiding pipe, so that it is convenient to observe by extracting the solution in the liquid collecting frame, so that the equipment can efficiently perform sampling, detection and processing of pine wood nematodes.

[0020] (2) In the present invention, when the sample wood is split, the generated debris is blocked by the guide frame, the card frame and the insert frame, so that the debris can fall into the second rotating frame. At this time, an appropriate amount of a solution of a certain temperature with an attractant added is injected into the guide frame, so that the solution can be injected into the second rotating frame through the guide frame. At this time, the linkage motor is controlled to start, so that the linkage motor cooperates with the two linkage gears to drive the linkage shaft to rotate, and then the linkage shaft cooperates with the second linkage wheel, the first linkage belt and the second linkage belt to drive the second linkage wheel. The first linkage wheel and the third linkage wheel rotate, thereby enabling the first linkage wheel and the third linkage wheel to drive the first rotating frame and the second rotating frame to rotate, thereby accelerating the entry of pine wood nematodes in the divided sample wood and debris into the solution, thereby improving the actual sampling efficiency of the equipment for pine wood nematodes, and then under the filtration of the filter tube, the solution containing pine wood nematodes can be injected into the liquid collecting frame through the filter tube, thereby facilitating subsequent solution extraction and observation, so that the equipment can efficiently and fully perform sampling and processing of pine wood nematodes, thereby improving the actual detection efficiency of pine wood nematodes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a first perspective stereogram of the present invention;

[0022] Figure 2 It is a second viewing angle stereogram of the present invention;

[0023] Figure 3 It is a cutaway stereogram of the present invention from a first viewing angle;

[0024] Figure 4 It is a cutaway perspective view of the collecting mechanism of the present invention;

[0025] Figure 5 It is a cross-sectional and expanded stereoscopic view of the collecting mechanism of the present invention;

[0026] Figure 6 For the present invention Figure 5 Enlarged view of part A in the middle;

[0027] Figure 7 For the present invention Figure 5 Enlarged view of part B;

[0028] Figure 8 It is a cutaway and expanded stereoscopic view of the separation mechanism of the present invention;

[0029] Fig. 9 For the present invention Figure 8 Enlarged view of part C in the middle;

[0030] Fig.10 For the present invention Figure 8 Enlarged view of part D in the middle;

[0031] Fig.11 For the present invention Figure 8 Enlarged view of part E in the middle;

[0032] Fig.12 For the present invention Figure 8 Enlarged view of part F in the middle;

[0033] Fig.13 For the present invention Figure 8 Enlarged view of part G in the middle.

[0034] Markings in the figure: 1. material collecting mechanism; 101. bottom frame; 102. liquid collecting frame; 103. linkage shaft; 104. linkage gear; 105. linkage motor; 106. second linkage wheel; 107. liquid rotating frame; 108. liquid guiding tube; 109. supporting threaded rod; 110. guide rod; 111. supporting worm; 112. supporting motor; 113. carrier; 114. elastic sealing ring; 115. partition frame; 116. first rotating frame; 117. paddle; 118. first linkage wheel; 119. first connecting frame; 120. inserting frame; 2. separation mechanism; 201. material guiding frame; 202. card frame; 203. stop Frame; 204, second connecting frame; 205, second rotating frame; 206, filter tube; 207, third linkage wheel; 208, top frame; 209, guide frame; 210, threaded tube; 211, movable worm; 212, movable motor; 213, movable threaded rod; 214, vehicle body; 215, rotating motor; 216, stop block; 217, rotating block; 218, rotating gear; 219, saw blade; 220, mounting frame; 221, bidirectional threaded rod; 222, limit motor; 223, limit worm; 224, splint; 225, reset frame; 226, clamping rod; 227, limit spring; 228, pressing ring. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0036] Example 1, please refer to Figure 1-Figure 3 A rapid sampling and detection device for pine wood nematode disease is composed of a collecting mechanism 1 and a separating mechanism 2.

[0037] The details are as follows:

[0038] See also Figure 4-Figure 7The collecting mechanism 1 includes a base frame 101, a liquid collecting frame 102, a supporting threaded rod 109, a carrier 113, a first separating component and a linkage component. The liquid collecting frame 102 is fixedly connected to the inner bottom surface of the base frame 101, the supporting threaded rod 109 is rotatably connected to the inner bottom surface of the base frame 101, the carrier 113 is threadedly connected to the outer surface of the supporting threaded rod 109, the first separating component is arranged on the carrier 113, and the linkage component is arranged on the liquid collecting frame 102. The inner bottom surface of the base frame 101 is fixedly connected with a guide rod 110, one end of the guide rod 110 slides through the bottom of the carrier 113, a supporting worm wheel is sleeved on the outer surface of the supporting threaded rod 109, the inner bottom surface of the base frame 101 is rotatably connected with a supporting worm 111, the supporting worm 111 is meshed with the supporting worm wheel, and the inner bottom surface of the base frame 101 is The surface is fixedly connected with a support motor 112, the output end of the support motor 112 is fixedly connected to one end of the support worm 111, an elastic sealing ring 114 is sleeved on the outer surface of the carrier 113, a partition frame 115 is placed on the top of the carrier 113, a placement groove is opened on the top of the carrier 113, the first separation component includes a first rotating frame 116, a first connecting frame 119 and an insertion frame 120, the first rotating frame 116 is slidably sleeved on the outer surface of the carrier 113, the outer surface of the first rotating frame 116 is sleeved with a first linkage wheel 118, a plurality of paddles 117 are equidistantly fixedly connected to the inner wall of the first rotating frame 116, a plurality of drainage pipes are equidistantly connected and arranged at the bottom of the first rotating frame 116, a sealing cover is threadedly connected to the bottom end of each drainage pipe, and a liquid transfer frame 107 is slidably sleeved on the outer surface of the first rotating frame 116, The bottom of the liquid transfer frame 107 is fixedly connected to the inner bottom surface of the base frame 101, and a liquid guide tube 108 runs through the inner bottom surface of the liquid transfer frame 107. One end of the liquid guide tube 108 extends to the inside of the liquid collecting frame 102. The first connecting frame 119 is rotatably connected to the outer surface of the first rotating frame 116. The first connecting frame 119 is inserted into the card frame 202. The insertion frame 120 is connected and arranged on the outer surface of one side of the first connecting frame 119, and one end of the insertion frame 120 extends to the outside of the material guide frame 201. The linkage component includes a linkage shaft 103 and a linkage motor 105. The linkage shaft 103 is rotatably connected to the top of the liquid collecting frame 102. The outer surface of the linkage shaft 103 is sleeved with a second linkage wheel 106 and a linkage gear 104. The outer surface of the second linkage wheel 106 and the outer surface of the first linkage wheel 118 are sleeved with a first linkage wheel 106. A linkage belt, a linkage motor 105 is fixedly connected to the top of the liquid collecting frame 102, and a linkage gear 104 is also sleeved on the output end of the linkage motor 105. The two linkage gears 104 are meshed, and the existing filter plate is placed through the placement groove. By controlling and starting the support motor 112, the support motor 112 cooperates with the support worm 111 and the support worm wheel to drive the support threaded rod 109 to rotate, and then under the position limit of the guide rod 110, the rotating support threaded rod 109 can increase the use height of the carrier 113, and then the processed sample wood is placed on the top of the partition 115, and the support motor 112 and the moving motor 212 are reversed to make the saw blade 219 gradually return to the original position, and the sample wood after the bottom is cut can follow the carrier 113 to gradually lower,When the top of the split wood sample is lower than the bottom height of the insert frame 120, another partition 115 is stacked and erected to the top of the split wood sample through the insert frame 120. At the same time, under the position restriction of the clamping rod 226, the remaining part of the wood sample will maintain its original position. At this time, the support motor 112, the moving motor 212 and the limit motor 222 are controlled to start, so that the newly placed partition 115 fits the bottom of the remaining wood sample again. At this time, the position restriction of the clamping rod 226 on the remaining wood sample is released, so that the carrier 113 can cooperate with the newly placed partition 115 to carry the remaining wood sample and descend. The saw blade 219 can then perform equidistant segmentation processing on the remaining sample wood, so that the sample wood can be segmented into multiple thinner wood chips. After the cyclic processing, the segmented sample wood is supported by the carrier 113 and finally stored in the first rotating frame 116 with equidistant intervals from the partition frame 115. At this time, an appropriate amount of a solution with a certain temperature added with an attractant is injected into the first rotating frame through the insertion frame 120 and the first connecting frame 119, so that the pine wood nematodes remaining in the thinner sample wood can be quickly lured out of the sample wood, and then the pine wood nematodes can follow the sample wood by removing the sealing cover. The solution is injected into the liquid transfer frame 107 through the drainage pipe, and then transported to the liquid collection frame 102 through the liquid guide pipe 108. During the sample wood segmentation process, the generated debris is blocked by the guide frame 201, the card frame 202 and the insertion frame 120, so that the debris can be concentrated and fall into the second rotating frame. At this time, an appropriate amount of a solution with a certain temperature added with an attractant is injected into the guide frame 201, so that the solution can be injected into the second rotating frame through the guide frame 201. At this time, the linkage motor 105 is started by control, so that the linkage motor 105 cooperates with the two linkage gears 104 to drive the linkage shaft 103 to rotate, and then The linkage shaft 103 cooperates with the second linkage wheel 106, the first linkage belt and the second linkage belt to drive the first linkage wheel 118 and the third linkage wheel 207 to rotate, and then the first linkage wheel 118 and the third linkage wheel 207 can drive the first rotating frame 116 and the second rotating frame 205 to rotate, thereby accelerating the entry of pine wood nematodes in the divided sample wood and debris into the solution, improving the actual sampling efficiency of the device for pine wood nematodes, and then under the filtration of the filter tube 206, the solution containing pine wood nematodes can be injected into the liquid collecting frame 102 through the filter tube 206, and then facilitate the subsequent extraction and observation of the solution;

[0039] See also Figure 8-Figure 13The separation mechanism 2 includes a material guide frame 201, a card frame 202, a top frame 208, a support frame 203, a support block 216, a vehicle body 214, a rotating block 217, a saw blade 219, a limiting component, an adjusting component and a second separation component. The material guide frame 201 is fixedly connected to the top of the bottom frame 101, the top frame 208 is arranged on the top of the material guide frame 201, and a moving port and a feeding port are opened on the top of the top frame 208. The card frame 202 is connected to the bottom surface of the inner part of the material guide frame 201, the support frame 203 is fixedly connected to the outer surface of one side of the card frame 202, the support block 216 is slidably inserted in the moving port, and the bottom of the support block 216 is attached to the top of the support frame 203, the vehicle body 214 is placed on the top of the top frame 208, and the bottom of the vehicle body 214 is fixedly connected to the top of the support block 216, and the rotating block 217 is fixedly connected to the outer surface of one side of the card frame 202. The sliding sleeve is arranged on the outer surface of the stop block 216, the saw blade 219 is arranged on the outer surface of the rotating block 217, the limiting component, the adjusting component and the second separating component are all arranged on the top frame 208, the adjusting component includes a guide frame 209, a threaded tube 210, a movable threaded rod 213, a movable worm 211, a movable motor 212, a rotating motor 215 and two rotating gears 218, the guide frame 209 is fixedly connected to the top of the top frame 208, the threaded tube 210 is rotatably connected to the outer surface of one side of the guide frame 209, the outer surface of the threaded tube 210 is sleeved with a movable worm gear, the movable threaded rod 213 is fixedly connected to the outer surface of one side of the vehicle body 214, and one end of the movable threaded rod 213 slides through the inner surface wall of one side of the guide frame 209, and the movable threaded rod 213 and the threaded tube 2 10 threaded connection, the mobile worm 211 is rotatably connected to the top of the guide frame 201, the mobile worm 211 is meshed with the mobile worm wheel, the mobile motor 212 is fixedly connected to the top of the top frame 208, the output end of the mobile motor 212 is fixedly connected to one end of the mobile worm 211, the rotating motor 215 is fixedly connected to the top of the vehicle body 214, and the output end of the rotating motor 215 extends to the bottom of the vehicle body 214, one of the rotating gears 218 is sleeved on the output end of the rotating motor 215, and the other rotating gear 218 is sleeved on the outer surface of the rotating block 217, and the two rotating gears 218 are meshed, and the limiting component includes a mounting frame 220, a bidirectional threaded rod 221, two clamping plates 224 and a limiting motor 222, and the mounting frame 220 is fixedly connected to the top of the top frame 208 The bidirectional threaded rod 221 is rotatably connected to the top of the mounting bracket 220, and a limiting worm gear is sleeved on the outer surface of the bidirectional threaded rod 221. Two clamping plates 224 are threadedly connected to the outer surface of the bidirectional threaded rod 221. A reset frame 225 is inserted on the outer surface of one side of each clamping plate 224. A clamping rod 226 is slidably inserted inside each reset frame 225. A pressure ring 228 is sleeved on the outer surface of each clamping rod 226. A limiting spring 227 is slidably sleeved on the outer surface of each clamping rod 226. The limiting motor 222 is fixedly connected to the top of the mounting bracket 220. A limiting worm 223 is sleeved on the output end of the limiting motor 222. The limiting worm 223 is meshed with the limiting worm gear. The second separation component includes a second connecting frame 204, a second rotating frame 205 and a filter tube 206.The second connecting frame 204 is connected and arranged at the bottom of the material guide frame 201, and the second rotating frame 205 is rotatably connected to the inside of the second connecting frame 204. The outer surface of the second rotating frame 205 is sleeved with a third linkage wheel 207, and a second linkage belt is sleeved between the outer surface of the third linkage wheel 207 and the outer surface of the second linkage wheel 106. The filter tube 206 runs through the inner bottom surface of the second rotating frame 205, and a sealing plug is inserted at the bottom end of the filter tube 206. By controlling and starting the limit motor 222, the limit motor 222 cooperates with the limit worm 223 and the limit worm gear to drive the bidirectional threaded rod 221 to rotate, and then the rotating bidirectional threaded rod 221 can drive the two clamps 224 to approach each other, so that the two adjacent clamps 224 can squeeze the fixed partition frame 1 through the clamp rod 226. 15 The top sample wood is placed at the position, at this time, the moving motor 212 is controlled to start, so that the moving motor 212 cooperates with the moving worm 211 and the moving worm wheel to drive the threaded tube 210 to rotate, and then the rotating threaded tube 210 cooperates with the moving threaded rod 213 to drive the vehicle body 214 to move in the horizontal direction. At the same time, the rotating motor 215 is controlled to start, so that the rotating motor 215 cooperates with the two rotating gears 218 to drive the rotating block 217 to rotate, and then the rotating rotating block 217 can drive the saw blade 219 to rotate. During the movement of the vehicle body 214, the vehicle body 214 cooperates with the stop block 216 and the rotating block 217 to drive the rotating saw blade 219 to gradually approach the sample wood, so that the sample wood can be efficiently and quickly split.

[0040] The following is a detailed description of a method for using a rapid sampling and detection device for pine wood nematode disease provided by an embodiment of the present invention, and the method for using the device comprises the following steps:

[0041] Step 1, preliminary extraction: Place the existing filter through the placement slot, control and start the support motor 112, so that the support motor 112 cooperates with the support worm 111 and the support worm gear to drive the support threaded rod 109 to rotate, and then under the position limit of the guide rod 110, the rotating support threaded rod 109 can increase the use height of the carrier 113, and then place the processed sample wood on the top of the partition 115, and then control and start the limit motor 222, so that the limit motor 222 cooperates with the limit worm 223 and the limit worm gear to drive the bidirectional threaded rod 221 to rotate, and then the rotating bidirectional threaded rod 221 can drive the two clamps 224 to approach each other, so that the two adjacent clamps 224 can squeeze and fix the partition through the clamp rod 226 115 is the top position where the sample wood is placed. At this time, the moving motor 212 is controlled to be started, so that the moving motor 212 cooperates with the moving worm 211 and the moving worm wheel to drive the threaded tube 210 to rotate, and then the rotating threaded tube 210 cooperates with the moving threaded rod 213 to drive the vehicle body 214 to move in the horizontal direction. At the same time, the rotating motor 215 is controlled to be started, so that the rotating motor 215 cooperates with the two rotating gears 218 to drive the rotating block 217 to rotate, and then the rotating rotating block 217 can drive the saw blade 219 to rotate. During the movement of the vehicle body 214, the vehicle body 214 cooperates with the stop block 216 and the rotating block 217 to drive the rotating saw blade 219 to gradually approach the sample wood, so that the sample wood can be quickly split efficiently. At the same time, the support motor 112 and the moving motor 212 are reversely operated to make the saw blade 219 gradually return to the original position, and the sample wood after the bottom segmentation can be gradually lowered with the carrier 113 until the top of the sample wood after segmentation is lower than the bottom height of the insert frame 120, and another partition frame 115 is stacked and erected to the top of the sample wood after segmentation through the insert frame 120. At the same time, under the position restriction of the clamping rod 226, the remaining part of the sample wood will maintain the original position. At this time, the support motor 112, the moving motor 212 and the limit motor 222 are controlled to start, so that the newly placed partition frame 115 fits the bottom of the remaining sample wood again. At this time, the position restriction of the clamping rod 226 on the remaining sample wood is released, so that the carrier 113 can support the newly placed partition frame 115. The remaining sample wood is lowered to a certain height, and then the saw blade 219 can perform equidistant segmentation processing on the remaining sample wood, so that the sample wood can be segmented into multiple thinner wood chips. After the cyclic processing, the segmented sample wood is supported by the carrier 113 and finally stored in the first rotating frame 116 with equidistant intervals from the partition frame 115. At this time, an appropriate amount of solution of a certain temperature added with an attractant is injected into the first rotating frame through the insertion frame 120 and the first connecting frame 119, so that the pine wood nematodes remaining in the thinner sample wood can be quickly lured out of the sample wood, and then the sealing cover is removed, so that the pine wood nematodes can be injected into the liquid transfer frame 107 through the drainage pipe with the solution, and then transported to the liquid collection frame 102 through the liquid guide pipe 108.It is convenient to observe by extracting the solution in the liquid collecting frame 102, so that the device can efficiently perform sampling, detection and processing of pine wood nematodes;

[0042] Step 2, efficient extraction: During the process of splitting the sample wood, the generated debris is blocked by the guide frame 201, the card frame 202 and the insertion frame 120, so that the debris can be concentrated and fall into the second rotating frame. At this time, an appropriate amount of a solution with a certain temperature added with an attractant is injected into the guide frame 201, so that the solution can be injected into the second rotating frame through the guide frame 201. At this time, the linkage motor 105 is started by control, so that the linkage motor 105 cooperates with the two linkage gears 104 to drive the linkage shaft 103 to rotate, and then the linkage shaft 103 cooperates with the second linkage wheel 106, the first linkage belt and the second linkage belt to drive The first linkage wheel 118 and the third linkage wheel 207 are driven to rotate, so that the first linkage wheel 118 and the third linkage wheel 207 can drive the first rotating frame 116 and the second rotating frame 205 to rotate, thereby accelerating the pine wood nematodes in the divided sample wood and debris to enter the solution, thereby improving the actual sampling efficiency of the equipment for pine wood nematodes, and then under the filtration of the filter tube 206, the solution containing pine wood nematodes can be injected into the liquid collecting frame 102 through the filter tube 206, and then it is convenient for the subsequent extraction and observation of the solution, so that the equipment can efficiently and fully perform the sampling and processing of pine wood nematodes, and improve the actual detection efficiency of pine wood nematodes.

[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A rapid sampling and detection device for pine wood nematode disease, characterized in that: include: A material collecting mechanism (1), the material collecting mechanism (1) comprising a base frame (101), a liquid collecting frame (102), a supporting threaded rod (109), a carrier (113), a first separation component and a linkage component, the liquid collecting frame (102) being fixedly connected to the inner bottom surface of the base frame (101), the supporting threaded rod (109) being rotatably connected to the inner bottom surface of the base frame (101), the carrier (113) being threadably connected to the outer surface of the supporting threaded rod (109), the first separation component being arranged on the carrier (113) and being used for separating and extracting pine wood nematodes, the linkage component being arranged on the liquid collecting frame (102) and being used for providing the rotational power required for quickly extracting pine wood nematodes; and A separation mechanism (2), the separation mechanism (2) comprising a material guide frame (201), a clamping frame (202), a top frame (208), a support frame (203), a support block (216), a vehicle body (214), a rotating block (217), a saw blade (219), a limiting component, an adjusting component and a second separation component, the material guide frame (201) being fixedly connected to the top of the bottom frame (101), the top frame (208) being arranged on the top of the material guide frame (201), a moving opening and a feeding opening being opened on the top of the top frame (208), the clamping frame (202) being arranged in communication with the inner bottom surface of the material guide frame (201), the support frame (203) being fixedly connected to the outer surface of one side of the clamping frame (202), the support block (216) being slidably inserted into the inner part of the moving opening, and the bottom of the support block (216) being in contact with the top of the support frame (203), the vehicle body (214), a rotating block (217), a saw blade (219), a limiting component, an adjusting component and a second separation component, the material guide frame (201) being fixedly connected to the top of the bottom frame (201), and the bottom of the support block (216) being in contact with the top of the support frame (203), and the vehicle body (214). The body (214) is placed on the top of the top frame (208), and the bottom of the body (214) and the top of the stop block (216) are fixedly connected. The rotating block (217) is slidably sleeved on the outer surface of the stop block (216), and the saw blade (219) is sleeved on the outer surface of the rotating block (217). The limiting component, the adjusting component and the second separation component are all arranged on the top frame (208). The limiting component is used to adjust the position state of the sample wood to be cut, and can be used in conjunction with the adjusting component to perform multiple splitting of the sample wood. The adjusting component is used to quickly split the sample wood, and can be used in conjunction with the limiting component to adjust the thickness of the sample wood after splitting. The second separation component is used to separate pine wood nematodes in smaller sample wood, and can be used in conjunction with the linkage component to synchronously perform rapid extraction of pine wood nematodes in the sample wood with the first separation component.

2. A rapid sampling and detection device for pine wood nematode disease according to claim 1, characterized in that: A guide rod (110) is fixedly connected to the inner bottom surface of the bottom frame (101), and one end of the guide rod (110) slides through the bottom of the carrier (113).

3. A rapid sampling and detection device for pine wood nematode disease according to claim 2, characterized in that: The outer surface of the supporting threaded rod (109) is sleeved with a supporting worm wheel, the inner bottom surface of the base frame (101) is rotatably connected to a supporting worm (111), the supporting worm (111) and the supporting worm wheel are meshed, the inner bottom surface of the base frame (101) is fixedly connected to a supporting motor (112), and the output end of the supporting motor (112) is fixedly connected to one end of the supporting worm (111).

4. A rapid sampling and detection device for pine wood nematode disease as claimed in claim 3, characterized in that: An elastic sealing ring (114) is sleeved on the outer surface of the carrier (113), a partition (115) is placed on the top of the carrier (113), and a placement groove is opened on the top of the carrier (113).

5. A rapid sampling and detection device for pine wood nematode disease according to claim 4, characterized in that: The first separation component comprises a first rotating frame (116), a first connecting frame (119) and an inserting frame (120); the first rotating frame (116) is slidably sleeved on the outer surface of the carrier (113); a first linkage wheel (118) is sleeved on the outer surface of the first rotating frame (116); a plurality of paddles (117) are equidistantly fixedly connected to the inner surface wall of the first rotating frame (116); a plurality of drainage pipes are equidistantly connected and arranged at the bottom of the first rotating frame (116); a sealing cover is threadedly connected to the bottom end of each drainage pipe; a rotating frame (100) is slidably sleeved on the outer surface of the first rotating frame (116); 7), the bottom of the liquid transfer frame (107) is fixedly connected to the inner bottom surface of the base frame (101), a liquid guide tube (108) passes through the inner bottom surface of the liquid transfer frame (107), one end of the liquid guide tube (108) extends to the inside of the liquid collecting frame (102), the first connecting frame (119) is rotatably connected to the outer surface of the first rotating frame (116), the first connecting frame (119) is inserted into the inside of the card frame (202), the inserting frame (120) is connected and arranged on the outer surface of one side of the first connecting frame (119), and one end of the inserting frame (120) extends to the outside of the material guiding frame (201).

6. A rapid sampling and detection device for pine wood nematode disease according to claim 5, characterized in that: The linkage component comprises a linkage shaft (103) and a linkage motor (105); the linkage shaft (103) is rotatably connected to the top of the liquid collecting frame (102); a second linkage wheel (106) and a linkage gear (104) are sleeved on the outer surface of the linkage shaft (103); a first linkage belt is sleeved between the outer surface of the second linkage wheel (106) and the outer surface of the first linkage wheel (118); the linkage motor (105) is fixedly connected to the top of the liquid collecting frame (102); an output end of the linkage motor (105) is also sleeved with a linkage gear (104); and the two linkage gears (104) are meshed.

7. A rapid sampling and detection device for pine wood nematode disease according to claim 6, characterized in that: The adjusting component comprises a guide frame (209), a threaded tube (210), a movable threaded rod (213), a movable worm (211), a movable motor (212), a rotating motor (215) and two rotating gears (218); the guide frame (209) is fixedly connected to the top of the top frame (208); the threaded tube (210) is rotatably connected to the outer surface of one side of the guide frame (209); a movable worm gear is sleeved on the outer surface of the threaded tube (210); the movable threaded rod (213) is fixedly connected to the outer surface of one side of the vehicle body (214); one end of the movable threaded rod (213) slides through the inner surface wall of one side of the guide frame (209); and the movable threaded rod (213) and the threaded tube (210) are threadedly connected. The movable worm (211) is rotatably connected to the top of the material guide frame (201), the movable worm (211) is meshed with the movable worm wheel, the movable motor (212) is fixedly connected to the top of the top frame (208), the output end of the movable motor (212) is fixedly connected to one end of the movable worm (211), the rotating motor (215) is fixedly connected to the top of the vehicle body (214), the output end of the rotating motor (215) extends to the bottom of the vehicle body (214), one of the rotating gears (218) is sleeved on the output end of the rotating motor (215), the other rotating gear (218) is sleeved on the outer surface of the rotating block (217), and the two rotating gears (218) are meshed.

8. A rapid sampling and detection device for pine wood nematode disease according to claim 7, characterized in that: The limiting component comprises a mounting frame (220), a bidirectional threaded rod (221), two clamping plates (224) and a limiting motor (222); the mounting frame (220) is fixedly connected to the top of the top frame (208); the bidirectional threaded rod (221) is rotatably connected to the top of the mounting frame (220); a limiting worm gear is sleeved on the outer surface of the bidirectional threaded rod (221); the two clamping plates (224) are both threadedly connected to the outer surface of the bidirectional threaded rod (221); and the outer surface of one side of each clamping plate (224) is A reset frame (225) is inserted in each of the reset frames (225), a clamping rod (226) is slidably inserted in the interior of each of the reset frames (225), a pressing ring (228) is sleeved on the outer surface of each of the clamping rods (226), and a limit spring (227) is slidably sleeved on the outer surface of each of the clamping rods (226), the limit motor (222) is fixedly connected to the top of the mounting frame (220), and a limit worm (223) is sleeved on the output end of the limit motor (222), and the limit worm (223) is meshed with the limit worm wheel.

9. A rapid sampling and detection device for pine wood nematode disease according to claim 8, characterized in that: The second separation component comprises a second connecting frame (204), a second rotating frame (205) and a filter tube (206); the second connecting frame (204) is connected to the bottom of the material guide frame (201); the second rotating frame (205) is rotatably connected to the inside of the second connecting frame (204); a third linkage wheel (207) is sleeved on the outer surface of the second rotating frame (205); a second linkage belt is sleeved between the outer surface of the third linkage wheel (207) and the outer surface of the second linkage wheel (106); the filter tube (206) passes through the inner bottom surface of the second rotating frame (205); and a sealing plug is inserted at the bottom end of the filter tube (206).

10. A method for using a rapid sampling and detection device for pine wood nematode disease, characterized in that: The device for rapid sampling and detection of pine wood nematode disease as claimed in claim 9 comprises the following steps: S1. Preliminary extraction: The existing filter sheet is placed in the placement slot, and the support motor (112) is controlled to be started, so that the support motor (112) cooperates with the support worm (111) and the support worm gear to drive the support threaded rod (109) to rotate, and then under the position limit of the guide rod (110), the rotating support threaded rod (109) can increase the use height of the carrier (113), and then the processed sample wood is placed on the top of the partition (115), and then the limit motor (222) is controlled to be started, so that the limit motor (222) cooperates with the limit worm (223) and the limit worm gear to drive the bidirectional threaded rod (221) to rotate, and then the rotating bidirectional threaded rod (221) can drive the two clamping plates (2 24) are brought close to each other, so that the two adjacent clamping plates (224) can squeeze and fix the sample wood placement position on the top of the partition frame (115) through the clamping rod (226). At this time, the moving motor (212) is controlled to be started, so that the moving motor (212) cooperates with the moving worm (211) and the moving worm wheel to drive the threaded tube (210) to rotate, and then the rotating threaded tube (210) cooperates with the moving threaded rod (213) to drive the vehicle body (214) to move in the horizontal direction. At the same time, the rotating motor (215) is controlled to be started, so that the rotating motor (215) cooperates with the two rotating gears (218) to drive the rotating block (217) to rotate, and then the rotating rotating block (217) can drive the saw blade (219) to rotate. During the movement of the vehicle body (214), the vehicle body (214) cooperates with the stop block (216) and the rotating block (217) to drive the rotating saw blade (219) to gradually approach the sample wood, thereby enabling the sample wood to be quickly and efficiently split. At this time, the support motor (112) and the moving motor (212) are reversely operated to enable the saw blade (219) to gradually return to its original position, and the sample wood after the split at the bottom can be gradually lowered along with the carrier (113), until the top of the split sample wood is lower than the bottom height of the insert frame (120), and another partition frame (115) is stacked and erected to the top of the split sample wood through the insert frame (120), and at the same time, under the position restriction of the clamping rod (226), the remaining part of the sample wood will maintain its original position. At this time, the support motor (112), the moving motor (212) and the limit motor (222) are controlled to start, so that the newly placed partition (115) is again attached to the bottom of the remaining sample wood. At this time, the position restriction of the remaining sample wood by the clamping rod (226) is released, so that the carrier (113) can cooperate with the newly placed partition (115) to carry the remaining sample wood and descend to a certain height, and then the saw blade (219) can perform equidistant segmentation processing on the remaining sample wood, so that the sample wood can be segmented into multiple thinner wood chips. In a cycle process, the segmented sample wood is supported by the carrier (113) and finally stored in the first rotating frame (116) at an equidistant interval from the partition (115).At this time, a proper amount of a solution of a certain temperature to which an attractant is added is injected into the first rotating frame through the insert frame (120) and the first connecting frame (119), so that the pine wood nematodes remaining in the thinner sample wood can be quickly lured away from the sample wood, and then by removing the sealing cover, the pine wood nematodes can be injected into the liquid transfer frame (107) along with the solution through the liquid discharge pipe, and then transported to the liquid collection frame (102) through the liquid guide pipe (108); S2. High-efficiency extraction: During the process of splitting the sample wood, the generated debris is blocked by the material guide frame (201), the clamp frame (202) and the insert frame (120), so that the debris can be concentrated and fall into the second rotating frame. At this time, an appropriate amount of a solution with a certain temperature added with an attractant is injected into the material guide frame (201), so that the solution can be injected into the second rotating frame through the material guide frame (201). At this time, the linkage motor (105) is controlled to start, so that the linkage motor (105) cooperates with the two linkage gears (104) to drive the linkage shaft (103) to rotate, and then the linkage shaft (103) cooperates with the second linkage gear (104) to rotate. The wheel (106), the first linkage belt and the second linkage belt can drive the first linkage wheel (118) and the third linkage wheel (207) to rotate, thereby enabling the first linkage wheel (118) and the third linkage wheel (207) to drive the first rotating frame (116) and the second rotating frame (205) to rotate, thereby accelerating the entry of pine wood nematodes in the divided sample wood and debris into the solution, thereby improving the actual sampling efficiency of the device for pine wood nematodes, and then under the filtration of the filter tube (206), the solution containing pine wood nematodes can be injected into the liquid collecting frame (102) through the filter tube (206), and then the extraction and observation of the solution are subsequently performed.

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