Cuttage breeding method for vaccinium yunnanense

Abstract

The invention discloses a cutting breeding method of Yunnan bilberry, which comprises the following steps: laying a heat preservation sand bed in a greenhouse, and monitoring the soil temperature in real time to keep the soil temperature at 18-19 DEG C; a matrix for cuttage is turf, vermiculite and coco coir according to the mixing ratio of 2: 1: 1, and the cuttage matrix needs to be sterilized and disinfected in advance; the method comprises the following steps: selecting a mother branch of the Yunnan bilberry, selecting an annual semi-lignified branch, and shearing the annual semi-lignified branch into a cutting short branch with the length of 5-10cm; quickly dipping the bottoms of the cuttings with 3 mg / L indoleacetic acid for 2-3 seconds; cuttage of the branches: obliquely inserting the cuttings subjected to root dipping treatment into cuttage hole trays, inserting a shearing opening into a cuttage medium by 4cm, enabling the shearing opening to be perpendicular to the horizontal direction, and tidily arranging the cuttage hole trays on a heat preservation sand bed. According to the method, the temperature of the soil is constant in a range suitable for rooting of the Yunnan bilberry cuttage branches in an electric heating mode, the rooting rate of cuttage seedlings can be greatly increased, and the difficulty of cuttage breeding is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of plant propagation, in particular to a cutting propagation method for Yunnan blueberry. Background Art

[0002] Yunnan blueberry is an evergreen shrub or small tree of the Ericaceae family and the genus Vaccinium. It has many branches, and the young branches are angular and glabrous. The leaves are leathery, ovate-lanceolate, oblong-lanceolate or ovate, with the midrib protruding on both sides, and the lateral veins are slender, slightly protruding on both sides, or flat and inconspicuous or slightly sunken on the surface. The inflorescence grows in the axils of the top and lower leaves of the branches, and the axis of the order is glabrous; the bracts are ovate or broadly ovate, with 2 bracteoles, ovate, and attached to the upper part of the pedicel close to the calyx tube. The pedicel is very short and glabrous. The calyx tube is spherical and glabrous, and the calyx teeth are triangular. The corolla is white or light red, tubular and peloid, with a slightly constricted mouth. The stamens are hidden, the filaments are sparsely pubescent, the anther loculus has a short spur on the back, and the anther tube is nearly as long as the anther loculus. The style slightly protrudes from the corolla, and the berries are purple-black when ripe. It is mainly distributed in southwestern Sichuan and southeastern to northwestern Yunnan, China, and grows in hillside shrubs or mountain evergreen broad-leaved forests, under pine and oak forests.

[0003] The uncontrollable soil temperature during the cutting propagation of Yunnan blueberry makes the Yunnan blueberry propagation method more difficult.

[0004] Therefore, how to provide a cutting propagation method by controlling the constant temperature of the soil bed is a technical problem that technicians in this field urgently need to solve. Summary of the invention

[0005] The purpose of the present invention is to provide a cutting propagation method for Vaccinium yunnanensis, which can greatly improve the rooting rate of cutting seedlings by keeping the soil temperature constant within a range suitable for the rooting of Vaccinium yunnanensis cuttings through electric heating, and solve the problems of high breeding difficulty, slow breeding speed and low success rate in the prior art.

[0006] The present invention solves the technical problem by adopting the following technical solution:

[0007] A cutting propagation method for Vaccinium yunnanensis comprises the following steps:

[0008] Step 1: Prepare the environment. Lay a heat-insulating sand bed under the location where the cutting plug tray is placed in the greenhouse. Lay a heating line at the bottom of the sand bed. Cover the heat-insulating sand bed with river sand to a thickness of 10 to 15 cm. Use a thermostat to monitor the temperature of the heat-insulating sand bed in real time. Use electric heating to keep the temperature of the heat-insulating sand bed constant at 18 to 19 degrees Celsius.

[0009] Step 2: Preparation and disinfection of cutting medium. The cutting medium is peat, vermiculite and coconut bran, with a mixing ratio of 2:1:1. The cutting medium needs to be sterilized in advance.

[0010] Step 3: Selection of Yunnan blueberry mother branches: select pest-free, 2-3 axillary buds, strong one-year-old semi-lignified branches, and cut them into short cuttings with a length of 5-10 cm;

[0011] Step 4: Root dipping treatment: dip the bottom of the cuttings quickly with 3mg / L indoleacetic acid for 2 to 3 seconds;

[0012] Step 5: For branch cuttings, insert the root-dipping cuttings obliquely into the cutting tray, with the cut end inserted 4 cm into the cutting medium and the cut end perpendicular to the horizontal direction. Arrange the cutting trays neatly on the insulating sand bed.

[0013] Furthermore, in the step 1, the temperature of the insulating sand bed is kept constant at 18-19° C. by electric heating, while the indoor temperature and humidity are monitored in real time.

[0014] Furthermore, the air humidity needs to be maintained between 90 and 95. When the air humidity is insufficient, an automatic sprayer is used for humidification. When the humidity reaches the required value, the spraying stops.

[0015] Furthermore, in the step 2, the cutting medium is disinfected by applying a 400% mass concentration of carbendazim wettable powder to the cutting medium for disinfection, placing the cutting tray on an insulation bed, covering the top of the greenhouse with a layer of shade net with a shading degree of 60% for shading, and installing fans on both sides of the greenhouse for ventilation so that the temperature in the greenhouse will not be too high.

[0016] Furthermore, in the step three, only 2 to 3 leaves need to be left on the short branches for cuttings, and 1 / 2 of the leaves that are too large should be cut off to prevent excessive water loss. The top of the cutting branch is cut into a flat end, and the wound is treated with grafting paint.

[0017] Furthermore, in the step 4, a 45-degree bevel is taken at the bottom of the cutting short branch for root dipping treatment.

[0018] Furthermore, in the step 5, the cutting hole tray after the cutting is completed needs to be neatly placed on a heat preservation bed with a constant temperature of 18 to 19° C., and needs to be watered once every morning to prevent the cutting medium from drying out and losing water.

[0019] The invention discloses a cutting propagation method for blueberry, which has the following beneficial effects:

[0020] (1) The present invention can not only save a large number of branches by shearing the existing bilberry mother branches, without affecting the growth of bilberry, but also increase the number of reproduction and accelerate the reproduction speed, and quickly dip the cutting branches with 3 mg / L indoleacetic acid for 2 to 3 seconds to improve the rooting rate of the mother branches. At the same time, the soil at the bottom of the cutting hole tray is heated to greatly increase the cutting propagation efficiency of bilberry and reduce the difficulty of cutting propagation.

[0021] (2) The present invention can greatly improve the rooting rate of Vaccinium vitis-inica cuttings by heating the soil at the roots of the mother branches of Vaccinium vitis-inica, and further increase the breeding speed of Vaccinium vitis-inica.

[0022] (3) The present invention has the characteristics of low breeding difficulty, fast breeding speed and high rooting success rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a flow chart of the cutting propagation method of the present invention. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example

[0026] like Figure 1 As shown, the present embodiment discloses a cutting propagation method of Vaccinium yunnanensis, comprising the following steps:

[0027] Step 1: Environmental preparation. Lay a heat preservation sand bed under the location where the cutting plug tray is placed in the greenhouse, lay a heating line at the bottom of the sand bed, and then cover the heat preservation sand bed with river sand to a thickness of 10-15 cm. Use a thermostat to monitor the temperature of the heat preservation sand bed in real time, and use electric heating to keep the temperature of the heat preservation sand bed constant at 18-19°C; at the same time, monitor the indoor temperature and humidity in real time; the air humidity needs to be maintained between 90 and 95. When the air humidity is not enough, use an automatic sprayer for humidification. When the humidity reaches the required value, the spraying stops. This ensures that the humidity in the greenhouse can be automatically regulated according to the rainy weather with low evaporation or the hot weather with high evaporation.

[0028] Step 2: Preparation and disinfection of cutting medium. The cutting medium is peat, vermiculite and coconut bran in a mixing ratio of 2:1:1. The cutting medium needs to be sterilized in advance. The cutting medium is disinfected by applying 400% carbendazim wettable powder to the cutting medium for disinfection. The cutting tray is placed on the insulation bed, and a layer of shade net with a shading degree of 60% is used to cover the top of the greenhouse for shading. Fans are installed on both sides of the greenhouse for ventilation to prevent the temperature in the greenhouse from being too high.

[0029] Step 3: Selection of mother branches of Yunnan blueberry. Choose healthy one-year-old semi-lignified branches that are free of pests and diseases, have 2 to 3 axillary buds, and are cut into short branches 5 to 10 cm in length for cuttings. Only 2 to 3 leaves need to be left on the short branches used for cuttings. Leaves that are too large should be cut off by 1 / 2 to prevent excessive water loss. Cut the upper part of the cutting branch into a flat end, treat the wound with grafting paint, and make a 45-degree bevel at the lower end for subsequent root dipping treatment.

[0030] Step 4: Root dip treatment: quickly dip the bottom of the cuttings with 3mg / L indoleacetic acid for 2-3 seconds. The indoleacetic acid used in the root dip treatment is a hormone reagent used to stimulate plant growth. It is widely used in agricultural production and has a dual effect on plant growth. Different parts of the plant have different sensitivities to it. Generally, roots are more sensitive than buds and stems, and different plants have different sensitivities to it.

[0031] Step 5: For branch cuttings, insert the root-dipping cuttings obliquely into the cutting tray, with the cut end inserted 4 cm into the cutting medium and the cut end perpendicular to the horizontal direction. Arrange the cutting trays neatly on the insulating sand bed; water immediately after cutting to control the moisture content of the medium to 60-75%. Water once every morning to prevent the cutting medium from drying out and losing water.

[0032] The present invention can not only save a large number of branches by shearing the existing mother branches of Yunnan blueberry, but also has no effect on the growth of Yunnan blueberry, and can increase the number of reproduction, accelerate the reproduction speed, and quickly dip the branches of cuttings with 3mg / L indoleacetic acid for 2-3 seconds, which greatly increases the cutting rooting rate of Yunnan blueberry. At the same time, heating the soil at the bottom of the cutting hole tray greatly increases the cutting breeding efficiency of Yunnan blueberry and reduces the difficulty of cutting breeding. The present invention places the cutting hole tray on a constant temperature bed with a temperature of 18-19 degrees, and heats the soil at the root of the mother branches of Yunnan blueberry, which can greatly improve the rooting rate of Yunnan blueberry cuttings and further increase the breeding speed of Yunnan blueberry. The present invention has the characteristics of low breeding difficulty, fast breeding speed and high rooting success rate.

[0033] Experimental example

[0034] The present invention discloses a cutting propagation method for bilberry. In order to verify the influence of a heated bed on the rooting rate of bilberry, a group which is placed in a heated bed at 18-19°C after being quickly dipped with 3 mg / L indoleacetic acid is set as an experimental group A, a group which is placed in a heated bed at 25-26°C after being quickly dipped with 3 mg / L indoleacetic acid is set as an experimental group B, a group which is placed in a heated bed at 15-16°C after being quickly dipped with 3 mg / L indoleacetic acid is set as an experimental group C, and a group without a heated bed is set as a control group. When the rest of the cutting propagation processes are the same, comparison is performed. The specific results are shown in Table 1.

[0035]

[0036]

[0037] Table 1

[0038] As shown in Table 1, the rooting time of mother branch cuttings after root dipping treatment with indoleacetic acid is faster and the rooting rate is higher when the temperature of the heating bed is 18-19°C.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cutting propagation method for Vaccinium yunnanensis, characterized in that: The steps include: Step 1: Prepare the environment. Lay a heat-insulating sand bed under the location where the cutting plug tray is placed in the greenhouse. Lay a heating line at the bottom of the sand bed. Cover the heat-insulating sand bed with river sand to a thickness of 10 to 15 cm. Use a thermostat to monitor the temperature of the heat-insulating sand bed in real time. Use electric heating to keep the temperature of the heat-insulating sand bed constant at 18 to 19 degrees Celsius. Step 2: Preparation and disinfection of cutting medium. The cutting medium is peat, vermiculite, and coconut bran, with a mixing ratio of 2:1:

1. The cutting medium needs to be sterilized in advance. Step 3: Selection of Yunnan blueberry mother branches: select pest-free, 2-3 axillary buds, strong one-year-old semi-lignified branches, and cut them into short cuttings with a length of 5-10 cm; Step 4: Root dipping treatment: dip the bottom of the cuttings quickly with 3mg / L indoleacetic acid for 2 to 3 seconds; Step 5: For branch cuttings, insert the root-dipping cuttings obliquely into the cutting tray, with the cut end inserted 4 cm into the cutting medium and the cut end perpendicular to the horizontal direction. Arrange the cutting trays neatly on the insulating sand bed.

2. The cutting propagation method of Vaccinium yunnanensis according to claim 1, characterized in that: In the step 1, the temperature of the insulating sand bed is kept constant at 18-19° C. by electric heating, while the indoor temperature and humidity are monitored in real time.

3. A cutting propagation method for Vaccinium yunnanensis according to claim 2, characterized in that: The air humidity needs to be maintained between 90 and 95. When the air humidity is not enough, use an automatic sprayer to humidify it. When the humidity reaches the required value, stop spraying.

4. A cutting propagation method for Vaccinium yunnanensis according to claim 3, characterized in that: In the step 2, the cutting medium is disinfected by applying a 400% mass concentration of carbendazim wettable powder to the cutting medium for disinfection, placing the cutting tray on the insulation bed, covering the top of the greenhouse with a layer of shade net with a shading degree of 60% for shading, and installing fans on both sides of the greenhouse for ventilation so that the temperature in the greenhouse will not be too high.

5. A cutting propagation method for Vaccinium yunnanensis according to claim 4, characterized in that: In the step three, only 2 to 3 leaves need to be left on the short branches for cuttings, and 1 / 2 of the leaves that are too large should be cut off to prevent excessive water loss. The top of the cutting branch is cut into a flat end, and the wound is treated with grafting paint.

6. The cutting propagation method of Vaccinium yunnanensis according to claim 4, characterized in that: In the step 4, a 45-degree bevel is taken at the bottom of the cutting short branch for root dipping.

7. The cutting propagation method of Vaccinium yunnanensis according to claim 4, characterized in that: In the step 5, the cutting hole tray after the cutting is completed needs to be neatly placed on a heat preservation bed with a constant temperature of 18 to 19° C., and water needs to be poured once every morning to prevent the cutting medium from drying out and losing water.

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