A method for the secondary fruiting of Phlebopus portentosus

Abstract

The present invention relates to the technical field of edible fungi. Specifically, it relates to a method for the secondary fruiting of Phlebopus portentosus. The method comprises the following steps: (1) raking the bacteria; (2) punching holes; (3) adding a nutrient supplement; (4) covering with soil; (5) fruiting management. The present invention can further transform and utilize the culture medium after the first fruiting, greatly improving the yield and reducing the production cost.

Description

Technical Field

[0001] The present invention relates to the technical field of edible fungi, and more specifically, to a method for the secondary fruiting of Phlebopus portentosus. Background Art

[0002] Phlebopus portentosus, also known as Phlebopus portentosus fuscus, commonly known as the black bolete, belongs to the order Boletales, the family Boletinellaceae, and the genus Phlebopus. Phlebopus portentosus is a very characteristic traditional wild mushroom ingredient in southern Yunnan, especially in the Xishuangbanna area. It has a unique flavor. During the cooking process, it does not become mushy after being cooked for a long time and always maintains the crispy, tender and smooth texture of the meat, with an excellent taste. At present, this species has been successfully cultivated artificially and can be mass-produced on an industrial scale.

[0003] In the current artificial cultivation of Phlebopus portentosus, the production process usually ends after the first fruiting. Given the relatively low biological conversion rate of Phlebopus portentosus itself, the culture medium after the first fruiting is not fully utilized, and there are still many nutrients remaining. Therefore, in the artificial cultivation of Phlebopus portentosus, how to make high-value use of the culture medium after the first fruiting is a topic worthy of in-depth research and an important way to further reduce costs and increase efficiency in its large-scale production. Summary of the Invention

[0004] In view of the above technical problems, the present invention provides a method for the secondary fruiting of Phlebopus portentosus, which can further transform and utilize the culture medium after its first fruiting, greatly increase the yield, and reduce the production cost.

[0005] To achieve the above object, the present invention mainly provides the following technical solutions:

[0006] A method for the secondary fruiting of Phlebopus portentosus, the method comprising the following steps:

[0007] (1) Scratching the mycelium;

[0008] (2) Punching holes;

[0009] (3) Adding a nutrient supplement;

[0010] (4) Covering with soil;

[0011] (5) Fruiting management.

[0012] Specifically, the mushroom bottles or bags of Phlebopus portentosus after the first fruiting are used for the secondary fruiting operation.

[0013] In step (1), through the operation of scraping the bacteria, the covering soil layer and the surface mycocuticle of the cultivation substrate in the bottle or bag of Boletus obscurinervius after the first fruiting are removed.

[0014] In step (2), holes are drilled in the cultivation substrate after removing the surface mycocuticle. The number of holes is 3 - 5, and the hole diameter is 0.5 - 0.8 cm.

[0015] In step (3), a nutritional supplement is added to the cultivation substrate after drilling. The addition amount of the nutritional supplement is as follows: for every 1 kg of cultivation substrate, 0.5 - 1 g of xylan, 0.1 - 0.3 g of yeast extract, 0.1 - 0.3 g of potassium dihydrogen phosphate, 0.1 - 0.3 g of magnesium sulfate, 0.1 - 0.2 g of xylanase, 0.05 - 0.1 g of amylase, and 50 - 100 ml of water are supplemented.

[0016] In step (4), soil is covered on the cultivation substrate after adding the nutritional supplement, and a mixed bacterial agent is added to the soil covering material used. The soil covering material in this step is unused soil covering material, which is a conventional soil covering material for the cultivation of Boletus obscurinervius and can be commercially purchased.

[0017] Specifically, the mixed bacterial agent added to the soil covering material is obtained by culturing bacteria using the soil covering material of the bottle or bag after the first fruiting.

[0018] The specific bacterial culturing is as follows: Select the soil covering material of the bottle or bag after the first fruiting, transfer it to a bacterial LB medium with a pH of 7.2, and culture it at 32°C for 2 days to obtain a mixed bacterial agent.

[0019] The composition of the bacterial LB medium is: peptone 10 g / L, yeast powder 5 g / L, NaCl 10 g / L.

[0020] In step (5), the fruiting management is carried out in stages: in the first stage, the carbon dioxide concentration in the cultivation environment is 800 - 1500 ppm, and it is cultured in dim light until the mycelium covers the soil covering layer; in the second stage, the carbon dioxide concentration in the cultivation environment is 300 - 800 ppm, and it is cultured in bright light to stimulate fruiting.

[0021] The present invention uses the bottle or bag of Boletus obscurinervius after the first fruiting. After steps of scraping the bacteria, drilling holes, adding a nutritional supplement, covering soil, and fruiting management, the fruiting bodies of Boletus obscurinervius for the second fruiting are obtained, further utilizing the culture medium after the first fruiting, increasing the yield, saving the cost of cultivation materials, and greatly improving the production efficiency. Detailed implementation mode

[0022] The technical solutions of the present invention are described in detail below in conjunction with embodiments.

[0023] Example 1 Comparison of the effects of different nutritional supplements

[0024] Use the bottle of Phlebopus portentosus after the first flush of fruiting to perform the second flush of fruiting operation:

[0025] (1) Remove the covering soil layer and the mycelial pellicle on the surface of the cultivation substrate in the bottle of the first flush of fruiting through the operation of scratching the mycelium.

[0026] (2) Punch holes in the cultivation substrate of the bottle after the first flush of fruiting. The number of holes is 3, and the hole diameter is 0.6 cm.

[0027] (3) Supplement the following different nutritional supplements to the substrate of each 1 kg bottle after the first flush of fruiting for a comparative experiment:

[0028] Supplement ①: 0.5 g of xylan, 0.1 g of yeast extract, 0.1 g of potassium dihydrogen phosphate, 0.1 g of magnesium sulfate, 0.1 g of xylanase, 0.05 g of amylase, 100 ml of water;

[0029] Supplement ②: 1 g of xylan, 0.3 g of yeast extract, 0.3 g of potassium dihydrogen phosphate, 0.3 g of magnesium sulfate, 0.2 g of xylanase, 0.1 g of amylase, 50 ml of water;

[0030] Supplement ③: 0.3 g of yeast extract, 0.3 g of potassium dihydrogen phosphate, 0.3 g of magnesium sulfate, 50 ml of water;

[0031] Control: 50 ml of water;

[0032] (4) Add a mixed bacterial agent to the covering soil material and cover the soil. The mixed bacterial agent is obtained by culturing bacteria using the covering soil material of the bottle or bag of the first flush of fruiting. The specific bacterial culture is as follows: Select the covering soil material of the bottle or bag after the first flush of fruiting, transfer it to the bacterial LB medium with a pH of 7.2, and culture it at 32 °C for 2 days to obtain the mixed bacterial agent. The composition of the bacterial LB medium is: 10 g / L of peptone, 5 g / L of yeast powder, and 10 g / L of NaCl.

[0033] (5) After covering the soil, the fruiting management is carried out in stages: In the first stage, the carbon dioxide concentration in the culture environment is 1200 ppm, and it is cultured in dim light until the mycelium covers the soil layer; in the second stage, the carbon dioxide concentration in the culture environment is 500 ppm, and it is cultured in bright light to stimulate fruiting.

[0034] (6) Data statistics:

[0035] Table 1 Effects of different nutritional supplements on the second flush of fruiting

[0036]

[0037] As can be seen from the above table, nutritional supplements ① and ② can effectively increase the average mushroom weight in the second flush of Phlebopus portentosus, and the fruiting rate reaches more than 94.5%.

[0038] Example 2 Effect of Adding Mixed Bacterial Agent

[0039] Use the Phlebopus portentosus mushroom bottle after the first fruiting for the second fruiting operation:

[0040] (1) Remove the covering soil layer and the mycelial cortex on the surface of the cultivation substrate in the mushroom bottle after the first fruiting through the operation of raking the mycelium.

[0041] (2) Drill holes in the substrate of the mushroom bottle after the first fruiting. The number of holes is 3, and the hole diameter is 0.6 cm.

[0042] (3) Supplement the following different nutritional supplements to every 1 kg of the substrate of the mushroom bottle after the first fruiting, and set two treatments:

[0043] Supplement ①: 0.5 g of xylan, 0.1 g of yeast extract, 0.1 g of potassium dihydrogen phosphate, 0.1 g of magnesium sulfate, 0.1 g of xylanase, 0.05 g of amylase, 100 ml of water;

[0044] Supplement ②: 1 g of xylan, 0.3 g of yeast extract, 0.3 g of potassium dihydrogen phosphate, 0.3 g of magnesium sulfate, 0.2 g of xylanase, 0.1 g of amylase, 50 ml of water;

[0045] (4) Prepare the mixed bacterial agent for covering soil:

[0046] Select the mushroom bottles with higher yields during the first fruiting, take samples of their covering soil materials, take 5 g of samples from each bottle, transfer the samples to the bacterial LB medium (g / L): peptone 10, yeast powder 5, NaCl 10, add water to make up to 1 L, pH 7.2, culture at 32 °C for 2 d, and obtain the mixed bacterial agent.

[0047] Add 5 ml / bottle of the above-mentioned mixed bacterial agent to the covering soil material and cover the soil. At the same time, set up a covering soil material without adding the mixed bacterial agent for comparison.

[0048] (5) After covering the soil, carry out fruiting management using stage management: in the first stage, the carbon dioxide concentration in the cultivation environment is 1200 ppm, and it is cultured in dim light until the mycelium fills the covering soil layer; in the second stage, the carbon dioxide concentration in the cultivation environment is 500 ppm, and it is cultured in bright light to stimulate fruiting.

[0049] (6) Data statistics:

[0050] Table 2 Effect of Adding Mixed Bacterial Agent on the Second Fruiting

[0051]

[0052] As can be seen from the above table, by adding the mixed bacterial agent for covering soil, the fruiting rate can be effectively improved, thereby increasing the yield.

[0053] The upper and lower limit values and interval values of the process parameters (such as temperature, time, etc.) of the present invention can all implement this method, and specific examples are not listed one by one here.

[0054] Conventional technical knowledge in the art can be adopted for the content not described in detail in the present invention.

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the embodiments, those of ordinary skill in the art should understand that any modification or equivalent replacement of the technical solutions of the present invention does not depart from the spirit and scope of the technical solutions of the present invention, and they should all be covered within the scope of the claims of the present invention.

Claims

1. A method for the secondary fruiting of Boletus phaeovenulosus, the method comprising the following steps: (1) Scratching the mycelium; (2) Punching holes; (3) Adding nutritional supplements: Supplement and add nutritional supplements to the cultivated substrate after punching holes. The addition amount of the nutritional supplements is as follows: for every 1 kg of the cultivated substrate, supplement 0.5 - 1 g of xylan, 0.1 - 0.3 g of yeast extract, 0.1 - 0.3 g of potassium dihydrogen phosphate, 0.1 - 0.3 g of magnesium sulfate, 0.1 - 0.2 g of xylanase, 0.05 - 0.1 g of amylase, and 50 - 100 ml of water; (4) Covering with soil: Cover the cultivated substrate supplemented with nutritional supplements with soil, and add a mixed bacterial agent to the soil covering material used; the mixed bacterial agent is obtained by culturing bacteria using the soil covering material of the mushroom bottle or mushroom bag after the first fruiting. (5) Fruiting management.

2. The method for the secondary fruiting of Phlebopus portentosus according to claim 1, characterized in that, In step (1), the soil covering layer and the surface myceliophane of the cultivated substrate in the mushroom bottle or mushroom bag of Boletus phaeovenulosus after the first fruiting are removed through the operation of scratching the mycelium.

3. The method for the secondary fruiting of Phlebopus portentosus according to claim 1, characterized in that, In step (2), holes are punched in the cultivated substrate after removing the surface myceliophane. The number of punched holes is 3 - 5, and the hole diameter is 0.5 - 0.8 cm.

4. The method for the secondary fruiting of Phlebopus portentosus as described in claim 1, characterized in that, The specific bacterial culture is as follows: Select the soil covering material of the mushroom bottle or mushroom bag after the first fruiting, transfer it to a bacterial LB medium with a pH of 7.2, and culture it at 32 °C for 2 days to obtain a mixed bacterial agent.

5. The method for the secondary fruiting of Phlebopus portentosus according to claim 4, wherein The composition of the bacterial LB medium is: peptone 10 g / L, yeast powder 5 g / L, NaCl 10 g / L.

6. The method for the second fruiting of Phlebopus portentosus according to claim 1, characterized in that, In step (5), the fruiting management adopts stage management: in the first stage, the carbon dioxide concentration in the cultivation environment is 800 - 1500 ppm, and it is cultured in dim light until the mycelium fills the soil covering layer; in the second stage, the carbon dioxide concentration in the cultivation environment is 300 - 800 ppm, and it is cultured in bright light to stimulate fruiting.

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