Preparation process of polygonatum sibiricum and wolfberry extract
Through a specific preparation process, the polysaccharide and flavonoid extraction rate of Polygonatum and Wolfberry extract is improved through specific preparation processes, and its nourishing effect is enhanced. Polygonatum and Wolfberry cream are prepared that can replenish essence and blood and strengthen qi.
Patent Information
- Application Number
- CN202411342965.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-25
- Publication Date
- 2025-07-11
- Estimated Expiration
- 2044-09-25
AI Technical Summary
In the prior art, the nourishing effect of the Polygonatum and wolfberry compositions is poor, so how to improve their nourishing effect.
A specific preparation process is adopted, including heating and extracting dried polina and wolfberry tablets with water, and adding dodecyl glucoside and ethanol during the heating process, irradiation treatment, and then concentrating to prepare polina and wolfberry extract.
By improving the dissolution efficiency and extraction rate of polysaccharides and flavonoids, the effect of Polygonatum and wolfberry extracts is enhanced to replenish essence, blood and strengthen qi.
Smart Images
Figure BDA0005060221910000061 
Figure BDA0005060221910000071 
Figure BDA0005060221910000072
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a preparation process of polygonatum and wolfberry extract. Background Art
[0002] The present invention discloses a dried rhizome of Polygonatum kingianum Coll. et Hemsl Delar. ex Redoute, a plant of the Liliaceae family. The rhizome is nearly cylindrical or nearly beaded, and the nodules are sometimes irregularly rhombus-shaped, hypertrophic, and 1-3 cm in diameter. The stem is 1-3 m high, and the top is in a climbing shape. The leaves are whorled, with 3-10 leaves per whorl, strip-shaped, strip-shaped wavy needle-shaped or lanceolate, 6-25 cm long, 3-30 mm wide, and fist-shaped at the tip. The inflorescence has 2-4 flowers, the peduncle is drooping, 1-2 cm long, the pedicel is 0.5-1.5 cm long, the bracts are membranous, tiny, and usually located at the bottom of the pedicel; the perianth is pink, 18-25 mm long, and the lobes are 3-5 mm long; the filaments are 3-5 mm long, filamentous or flat on both sides, and the anthers are 4-6 mm long; the ovary is 4-6 mm long, and the style is 10-14 mm long. The berries are red, 1-1.5 cm in diameter, and contain 7-12 seeds. The flowering period is from March to May, and the fruiting period is from September to October.
[0003] Yunnan Polygonatum, a medicinal and edible herb, is the dried rhizome of Yunnan Polygonatum, a plant of the Liliaceae family. It is sweet and neutral in nature. It enters the spleen, lung, and kidney meridians. It has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. It is used for spleen and stomach qi deficiency, fatigue, insufficient stomach yin, dry mouth and poor appetite, dry cough due to lung deficiency, hemoptysis due to fatigue, insufficient essence and blood, soreness of the waist and knees, premature graying of hair, internal heat and thirst, etc.
[0004] Wolfberry (Lycium chinenseMiller), a perennial woody plant of the genus Lycium in the Solanaceae family, has thin, weak, bowed or drooping branches, and is light gray; the leaves are oval; the flowers grow solitary or in pairs in the axils of leaves on long branches; the fruit is an oval berry red; the flowering period is June-July; the fruiting period is August-October.
[0005] Chinese medicine wolfberry is the dried mature fruit of the Solanaceae plant wolfberry, which is flat in nature and sweet in taste. It enters the liver meridian, kidney meridian and lung meridian. It has the effects of nourishing the liver and kidney, improving sperm and improving eyesight. It is used for liver and kidney yin deficiency, soreness of waist and knees, dizziness, blurred vision and excessive tears, consumptive cough, thirst, spermatorrhea and other symptoms.
[0006] The prior art has been about the combined use of polygonatum and wolfberry for tonifying the spleen, moistening the lungs, promoting the production of body fluids, calming the nerves, and replenishing deficiency, but the tonic effect is poor. How to improve the tonic effect of the polygonatum and wolfberry combination is a problem that technicians in this field need to solve. Summary of the invention
[0007] The object of the present invention is to provide a preparation process of polygonatum sibiricum and wolfberry extract. By adopting a specific preparation process, the active ingredients in polygonatum sibiricum and wolfberry are fully extracted, thereby improving the tonic effect of the polygonatum sibiricum and wolfberry composition.
[0008] To achieve the above object, the present invention provides the following technical solutions:
[0009] One of the technical solutions of the present invention: provides a preparation process of polygonatum sibiricum and wolfberry extract, including the following steps:
[0010] Add the dried and pulverized polygonatum kingianum slices and wolfberry slices into water, add dodecyl glucoside, heat for extraction, and irradiate while heating for extraction, then add ethanol, heat for extraction, and irradiate while heating for extraction, heat the extract to remove ethanol, and then concentrate to obtain the polygonatum sibiricum and wolfberry extract.
[0011] Preferably, the mass ratio of the polygonatum kingianum slices to the wolfberry slices is 10-15:5-15.
[0012] Preferably, the addition amount of water is 20-30 times the total mass of the polygonatum kingianum slices and wolfberry slices.
[0013] Preferably, the addition amount of dodecyl glucoside is 2-3% of the total mass of the polygonatum kingianum slices and wolfberry slices.
[0014] Preferably, the temperature for heating extraction after adding water is 95-100 °C, and the time is 45-75 min.
[0015] Preferably, the dosage of irradiation during heating extraction with water as the extract is 9-11 kGy.
[0016] In the examples of the present invention, the irradiation dose of 10 kGy has little difference in effect from 9 or 11 kGy.
[0017] Preferably, the addition amount of ethanol is to adjust the ethanol content of the extract to 70-75 vol.%.
[0018] More preferably, the temperature for heating extraction after adding ethanol is 70-80 °C, and the time is 45-75 min.
[0019] Preferably, the dosage of irradiation during heating extraction after adding ethanol is 6-8 kGy.
[0020] In the examples of the present invention, the irradiation dose of 7 kGy has little difference in effect from 6 or 8 kGy.
[0021] Another technical solution provided by the present invention: provides a polygonatum sibiricum and wolfberry paste, which is prepared by mixing the polygonatum sibiricum and wolfberry extract obtained by the above preparation process with honey.
[0022] The beneficial technical effects of the present invention are as follows:
[0023] The present invention provides a preparation process for polygonatum and wolfberry extract. By adding dodecyl glucoside during the extraction process, the composite carbon contained therein is used to reduce the solid-liquid interface tension, increase the dissolution efficiency of polysaccharides and flavonoid compounds, and thus improve the extraction rate. Further, by introducing irradiation treatment during the extraction process, not only the extraction rate of polysaccharides and flavonoids is enhanced, but also the effectiveness of the polysaccharides obtained by extraction is improved.
[0024] The polygonatum and wolfberry extract obtained by the preparation process of the present invention can be directly mixed with honey to prepare an edible polygonatum and wolfberry paste, and the obtained polygonatum and wolfberry paste has the effects of tonifying essence and blood and consolidating qi. Specific embodiments
[0025] Now, various exemplary embodiments of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention. It should be understood that the terms described in the present invention are only used to describe specific embodiments and are not used to limit the present invention.
[0026] In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0027] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention.
[0028] Regarding the terms "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.
[0029] The polygonatum kingianum and wolfberry slices used in the examples and comparative examples of the present invention are all traditional Chinese medicine slices of the same origin and the same batch, and the polygonatum kingianum slices are polygonatum kingianum slices processed by steaming and boiling nine times.
[0030] Example 1
[0031] Preparation of polygonatum and wolfberry extract:
[0032] The Polygonatum kingianum Coll. et Hemsl. slices and Lycium barbarum slices dried at 60°C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of the Lycium barbarum slices were taken, 500 mL of water was added, and then 0.5 g of dodecyl glucoside was added. They were refluxed and extracted at 100°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 10 kGy. After cooling to below 60°C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%. Then it was refluxed and extracted at 80°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 7 kGy. After refluxing and extracting, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78°C, and then concentrated at 100°C to a relative density of 1.3 mg / mL, thus obtaining the Polygonatum kingianum Coll. et Hemsl. and Lycium barbarum extract.
[0033] Example 2
[0034] Preparation of the Polygonatum kingianum Coll. et Hemsl. and Lycium barbarum extract:
[0035] The Polygonatum kingianum Coll. et Hemsl. slices and Lycium barbarum dried at 60°C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of the Lycium barbarum slices were taken, 500 mL of water was added, and then 7 g of dodecyl glucoside was added. They were refluxed and extracted at 100°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 10 kGy. After cooling to below 60°C, ethanol was added to adjust the ethanol content in the extract to 75 vol.%. Then it was refluxed and extracted at 80°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 7 kGy. After refluxing and extracting, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78°C, and then concentrated at 100°C to a relative density of 1.3 mg / mL, thus obtaining the Polygonatum kingianum Coll. et Hemsl. and Lycium barbarum extract.
[0036] Example 3
[0037] Preparation of the Polygonatum kingianum Coll. et Hemsl. and Lycium barbarum extract:
[0038] The Polygonatum kingianum Coll. et Hemsl. slices and Lycium barbarum dried at 60°C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of the Lycium barbarum slices were taken, 500 mL of water was added, and then 0.6 g of dodecyl glucoside was added. They were refluxed and extracted at 100°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 10 kGy. After cooling to below 60°C, ethanol was added to adjust the ethanol content in the extract to 75 vol.%. Then it was refluxed and extracted at 80°C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator, and the irradiation dose was 7 kGy. After refluxing and extracting, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78°C, and then concentrated at 100°C to a relative density of 1.3 mg / mL, thus obtaining the Polygonatum kingianum Coll. et Hemsl. and Lycium barbarum extract.
[0039] Comparative Example 1
[0040] Preparation of polygonatum sibiricum and wolfberry extract (the difference from Example 1 is that the addition of dodecyl glucoside is omitted):
[0041] The processed polygonatum kingianum slices and wolfberries dried at 60 °C were respectively pulverized. 15 g of the pulverized polygonatum kingianum slices and 10 g of wolfberry slices were taken, added with 500 mL of water, and reflux-extracted at 100 °C for 60 min. During the reflux extraction, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 10 kGy. After cooling to below 60 °C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%, and then reflux-extracted at 80 °C for 60 min. During the reflux extraction, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 7 kGy. After the reflux extraction, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78 °C, and then concentrated at 100 °C to a relative density of 1.3 mg / mL, thus obtaining the polygonatum sibiricum and wolfberry extract.
[0042] Comparative Example 2
[0043] Preparation of polygonatum sibiricum and wolfberry extract (the difference from Example 1 is that the irradiation doses selected for the two heating refluxes are both 10 kGy):
[0044] The processed polygonatum kingianum slices and wolfberries dried at 60 °C were respectively pulverized. 15 g of the pulverized polygonatum kingianum slices and 10 g of wolfberry slices were taken, added with 500 mL of water, and 0.5 g of dodecyl glucoside was further added. It was reflux-extracted at 100 °C for 60 min. During the reflux extraction, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 10 kGy. After cooling to below 60 °C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%, and then reflux-extracted at 80 °C for 60 min. During the reflux extraction, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 10 kGy. After the reflux extraction, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78 °C, and then concentrated at 100 °C to a relative density of 1.3 mg / mL, thus obtaining the polygonatum sibiricum and wolfberry extract.
[0045] Comparative Example 3
[0046] Preparation of polygonatum sibiricum and wolfberry extract (the difference from Example 1 is that the irradiation doses selected for the two heating refluxes are both 7 kGy):
[0047] The Polygonatum kingianum Coll. et Hemsl. slices and wolfberries dried at 60 °C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of wolfberry slices were taken, 500 mL of water was added, and then 0.5 g of dodecyl glucoside was added. They were refluxed and extracted at 100 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 7 kGy. After cooling to below 60 °C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%. Then, it was refluxed and extracted at 80 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 7 kGy. After refluxing and extracting, it was cooled and filtered. The ethanol in the filtrate was first distilled out at 78 °C, and then concentrated at 100 °C to a relative density of 1.3 mg / mL to obtain the extract of Polygonatum kingianum Coll. et Hemsl. and wolfberry.
[0048] Comparative Example 4
[0049] Preparation of the extract of Polygonatum kingianum Coll. et Hemsl. and wolfberry (the difference from Example 1 is that the irradiation doses selected for the two heating refluxes are both 15 kGy):
[0050] The Polygonatum kingianum Coll. et Hemsl. slices and wolfberries dried at 60 °C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of wolfberry slices were taken, 500 mL of water was added, and then 0.5 g of dodecyl glucoside was added. They were refluxed and extracted at 100 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 15 kGy. After cooling to below 60 °C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%. Then, it was refluxed and extracted at 80 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 15 kGy. After refluxing and extracting, it was cooled and filtered. The ethanol in the filtrate was first distilled out at 78 °C, and then concentrated at 100 °C to a relative density of 1.3 mg / mL to obtain the extract of Polygonatum kingianum Coll. et Hemsl. and wolfberry.
[0051] Comparative Example 5
[0052] Preparation of the extract of Polygonatum kingianum Coll. et Hemsl. and wolfberry (the difference from Example 1 is that the irradiation doses selected for the two heating refluxes are both 5 kGy):
[0053] The processed Polygonatum kingianum Coll. et Hemsl. slices and wolfberries dried at 60 °C were respectively pulverized. 15 g of the pulverized Polygonatum kingianum Coll. et Hemsl. slices and 10 g of the wolfberry slices were taken, 500 mL of water was added, and then 0.5 g of dodecyl glucoside was added. The mixture was refluxed and extracted at 100 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 5 kGy. After cooling to below 60 °C, ethanol was added to adjust the ethanol content in the extract to 70 vol.%. The mixture was continuously refluxed and extracted at 80 °C for 60 min. While refluxing and extracting, electron irradiation was carried out using a high-energy electron accelerator with an irradiation dose of 5 kGy. After refluxing and extracting, it was cooled and filtered. The filtrate was first distilled to remove ethanol at 78 °C, and then concentrated at 100 °C to a relative density of 1.3 mg / mL, thus obtaining the extract of Polygonatum kingianum Coll. et Hemsl. and wolfberries.
[0054] The extracts of Polygonatum kingianum Coll. et Hemsl. and wolfberries prepared in Examples 1-3 and Comparative Examples 1-5 were weighed, and the results are shown in Table 1.
[0055] Table 1
[0056]
[0057] The total weight of total flavonoids and the total weight of total polysaccharides in the extracts of Polygonatum kingianum Coll. et Hemsl. and wolfberries prepared in Examples 1-3 and Comparative Examples 1-5 were determined. The determination results are shown in Table 2. The determination method was to lyophilize the extracts of each group, and the obtained samples after drying were respectively subjected to the determination of the total flavonoid content and the total polysaccharide content, and the total weight of total flavonoids and the total weight of total polysaccharides in the extracts were calculated; among them, the determination of the total flavonoid content was carried out using rutin as a reference substance by the aluminum chloride colorimetric method, and the total polysaccharide content was determined by the phenol-sulfuric acid method.
[0058] Table 2
[0059]
[0060] It can be seen from Table 2 that if the addition of dodecyl glucoside is omitted, the extraction rates of both total flavonoids and total polysaccharides will decrease. Changing the irradiation dose during extraction will also affect the extraction rates of total flavonoids and total polysaccharides.
[0061] The antioxidant activities of the dried products of the extracts of Polygonatum kingianum Coll. et Hemsl. and wolfberries prepared in Examples 1-3 and Comparative Examples 1-5 were determined.
[0062] Determination of the scavenging ability of hydroxyl radicals (·OH): 1 g of the lyophilized product of the extract of each group was taken, dissolved in 1 L of 30 vol.% ethanol solution, 0.5 mL of the prepared solution was taken, and 1 mL of 6 mmol·L -1 ferrous sulfate, 2 mL of 6 mmol·L -1 hydrogen peroxide, and 1 mL of 6 mmol·L -1 salicylic acid were added, mixed well, and reacted in a water bath at 37 °C for 30 min; distilled water was used to zero the absorbance, and the absorbance value was measured at 510 nm and recorded as A样品 ; Distilled water was used to replace the extraction solution, denoted as A 空白 ; Distilled water was used to replace hydrogen peroxide, denoted as A 对照 ; The scavenging rate of ·OH was calculated according to formula (1), and the calculation results are shown in Table 4.
[0063] Determination of the scavenging ability of DPPH free radicals (DPPH·): Take 1 g of the freeze-dried product of the extract of each group, dissolve it in 1 L of 30 vol.% ethanol solution. Take 0.5 mL of the prepared solution and add 3.5 mL of 0.5 mmol·L -1 DPPH solution, mix well, and let it stand in the dark at room temperature for 30 min; Zero with absolute ethanol, and measure the absorbance at 517 nm, denoted as A 样品 ; Distilled water was used to replace the extraction solution, denoted as A 空白 ; Absolute ethanol was used to replace DPPH, denoted as A 对照 ; The scavenging rate of DPPH· was calculated according to formula (1), and the calculation results are shown in Table 4.
[0064] Scavenging rate (%) = [A 空白 -(A 样品 -A 对照 )] / A 空白 ×100% Formula (1)
[0065] Table 3 Activity of scavenging hydroxyl radicals by the dried products of the extracts of each group
[0066]
[0067]
[0068] Table 4 Activity of scavenging DPPH free radicals by the dried products of the extracts of each group
[0069]
[0070] It can be seen from the results of Table 3 and Table 4 that for the experimental groups without irradiation or with too large or too small irradiation dose, the antioxidant capacity of the obtained samples was significantly reduced, indicating that irradiation with the correct dose is helpful to improve the antioxidant effect of the product. The main source of the antioxidant capacity of the freeze-dried product of the extract is polysaccharide. The antioxidant data of Comparative Example 2 and Examples 1-3 show that the irradiation dose of 9-11 kGy mainly affects the effectiveness of polysaccharide.
[0071] The above-described embodiments are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.
Claims
1. A preparation process of polygonatum sibiricum and wolfberry extract, characterized in that, It includes the following steps: Add the dried and pulverized Polygonatum kingianum slices and Lycium barbarum slices into water, add dodecyl glucoside, heat for extraction, and perform irradiation while heating for extraction. Then add ethanol and heat for extraction, and perform irradiation while heating for extraction. Heat the extract to remove ethanol, and then concentrate it to obtain the Polygonatum kingianum and Lycium barbarum extract paste; When using water as the extraction solution and heating for extraction, the irradiation dose is 9 - 11 kGy; When adding ethanol and heating for extraction, the irradiation dose is 6 - 8 kGy.
2. The preparation process of the polygonatum and wolfberry extract according to claim 1, characterized in that, The mass ratio of the Polygonatum kingianum slices to the Lycium barbarum slices is 10 - 15:5 - 15.
3. The preparation process of the polygonatum and wolfberry extract according to claim 1 is characterized in that, The addition amount of water is 20 - 30 times the total mass of the Polygonatum kingianum slices and Lycium barbarum slices.
4. The preparation process of the polygonatum and wolfberry extract according to claim 1, characterized in that, The addition amount of dodecyl glucoside is 2 - 3% of the total mass of the Polygonatum kingianum slices and Lycium barbarum slices.
5. The preparation process of the polygonatum and wolfberry extract according to claim 1, characterized in that, The temperature for heating extraction after adding water is 95 - 100 °C, and the time is 45 - 75 min.
6. The preparation process of the polygonatum and wolfberry extract according to claim 1, characterized in that, The addition amount of ethanol is to adjust the ethanol content of the extraction solution to 70 - 75 vol.%.
7. The preparation process of the polygonatum and wolfberry extract according to claim 6, characterized in that, The temperature for heating extraction after adding ethanol is 70 - 80 °C, and the time is 45 - 75 min.
8. A polygonatum and wolfberry paste, characterized in that, It is prepared by mixing the Polygonatum kingianum and Lycium barbarum extract paste prepared by the preparation process according to any one of claims 1 - 7 with honey.