A diuretic fresh Chinese medicinal composition

Abstract

The present invention provides a diuretic fresh Chinese herbal medicine composition, relating to the technical field of fresh Chinese herbal medicine pharmaceutical manufacturing. The present invention adheres to the theory of traditional Chinese medicine and the principle of treating symptoms based on syndrome differentiation, resulting in a fresh Chinese herbal medicine composition consisting of fresh wax gourd seeds, fresh lotus leaves, fresh dianthus root, fresh salvia miltiorrhiza, and fresh alisma orientalis. The fresh Chinese herbal medicine composition of the present invention has significant diuretic and anti-inflammatory effects, and its therapeutic effect is significantly superior to that of freeze-dried and processed products, comparable to that of hydroflumethiazide, making it a suitable alternative to Western medicine. Furthermore, the various components of the fresh Chinese herbal medicine composition of the present invention synergistically enhance their effectiveness, and none of them can be omitted. The fresh Chinese herbal medicine composition of the present invention has no toxic side effects in mice and is safe and effective. The fresh Chinese herbal medicine composition of the present invention provides new ideas for the development and application of diuretics.

Description

Technical Field

[0001] The invention relates to the technical field of fresh Chinese medicinal preparations, in particular to a diuretic fresh Chinese medicinal composition. Background Art

[0002] The urinary system is an important excretory system in the human body. Its main function is excretion, regulating the body's water and salt metabolism and acid-base balance, producing a variety of biologically active substances, and playing a vital role in maintaining the homeostasis of the body's internal environment. Urinary system diseases mainly include diseases of the kidneys, ureters, bladder, prostatitis, and urethra. Most urinary system diseases are caused by infection and are also infectious diseases. They are prone to recurrence and difficult to cure. Its clinical manifestations mainly include symptoms such as frequent urination, urgency, pain when urinating, oliguria, polyuria, hematuria, edema, etc. At present, the treatment of urinary system diseases is mainly based on the treatment of the primary disease, combined with diuretics such as diuretics and linshitonglin.

[0003] Diuretics are commonly used clinically. They act directly on the kidneys, increasing electrolyte and water excretion and urine output. Commonly used diuretics fall into five main categories: loop diuretics, thiazide diuretics, potassium-sparing diuretics, carbonic anhydrase diuretics, and osmotic diuretics. While these diuretics offer significant efficacy, they do not address the underlying cause. Long-term use can cause severe side effects and irreversible organ damage. Traditional Chinese Medicine (TCM) is a life science with Chinese characteristics and a unique health resource for my country. TCM has long recognized the importance of diuretics for diuresis and stranguria, as detailed in renowned medical texts such as the Yellow Emperor's Classic of Internal Medicine and the Compendium of Materia Medica. After thousands of years of medical experience, TCM diuretics have gained widespread recognition and application.

[0004] Fresh Chinese medicinal materials refer to fresh plants (including roots, stems, leaves, flowers, and fruits) and live animals (including whole animals, organs, and tissues), which have not been dried or processed. These herbs are used directly to treat illnesses under the guidance of Traditional Chinese Medicine (TCM). Because they remain unprocessed, fresh herbs retain their natural medicinal properties. The use of fresh herbs has a long history, dating back to Shennong's tasting of herbs, when medicine generally referred to "fresh herbs." Fresh herbs refer to herbs used freshly harvested, retaining their active ingredients and providing superior efficacy. Research has shown that many fresh herbs have a fragrant aroma and a light, refreshing nature. They possess properties such as quenching thirst, promoting salivation, harmonizing the spleen and eliminating dampness, and dispelling turbidity and relieving heat. They can treat acute, febrile, and superficial symptoms, and their efficacy differs significantly from dried or processed herbs. Modern medicine believes that fresh herbs, due to their freshness and unprocessed nature, retain their natural state and molecular structure, resulting in high levels of active ingredients and superior efficacy. A large amount of modern research data also shows that the chemical composition of fresh herbs is significantly different from that of processed dried herbs, and the content of some active ingredients is often higher. This also reveals the scientific nature of the fresh use of Chinese medicine and the rationality of its clinical use from the perspective of the material basis of the herbs.

[0005] Based on the above research background, the present invention aims to provide a traditional Chinese medicine fresh medicine composition with definite efficacy, small toxic and side effects, and diuretic and stranguria-relieving effects. Summary of the Invention

[0006] (1) Technical problems solved

[0007] In view of the deficiencies in the prior art, the present invention provides a traditional Chinese medicine fresh medicine composition with diuretic and stranguria-relieving effects.

[0008] (2) Technical solution

[0009] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0010] The invention provides a diuretic fresh Chinese medicinal composition. The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 10-25 parts of fresh wax gourd seeds, 10-25 parts of fresh lotus leaves, 5-20 parts of fresh dianthus, 5-20 parts of fresh salvia miltiorrhiza, and 5-20 parts of fresh alisma orientalis.

[0011] Furthermore, the fresh Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wax gourd seeds, 10-20 parts of fresh lotus leaves, 5-15 parts of fresh dianthus, 5-15 parts of fresh salvia miltiorrhiza, and 5-15 parts of fresh alisma orientalis.

[0012] Furthermore, the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh dianthus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0013] Specifically, in the described traditional Chinese medicine fresh medicine composition, fresh wax gourd seeds are seeds of Benincas ahispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh Dianthus superbus L. is the whole herb of the perennial herb Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh Salvia miltiorrhiza is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh Alisma plantago is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alismataceae family. The above medicinal herbs are freshly picked when used and are not processed or prepared in any way.

[0014] When used, the fresh Chinese medicinal composition of the present invention is weighed according to the above-mentioned parts by weight, mashed into juice and taken.

[0015] The pharmacological effects of each Chinese herbal medicine in the Chinese herbal medicine fresh medicine composition of the present invention are as follows:

[0016] Winter melon seeds: sweet in taste, cool in nature; have the effects of moistening the lungs, resolving phlegm, eliminating carbuncle, and promoting diuresis; used for phlegm-heat cough, lung abscess, intestinal abscess, gonorrhea, edema, athlete's foot, hemorrhoids, nasal and facial alcohol, etc.

[0017] Lotus leaf: bitter in taste, neutral in nature, enters the liver, spleen, and stomach meridians; has the effects of clearing away heat and dampness, promoting the development of clear yang, cooling blood and stopping bleeding; used for symptoms such as summer heat and thirst, summer heat and dampness diarrhea, spleen deficiency diarrhea, blood heat vomiting and epistaxis, and bloody stool and metrorrhagia.

[0018] Dianthus: bitter in taste, cold in nature, enters the heart, kidney, small intestine and bladder meridians; has the effects of clearing heat, promoting diuresis, breaking up blood and dredging menstruation; treats urinary obstruction, gonorrhea, edema, amenorrhea, carbuncle, red eyes and cataract, and ulcers.

[0019] Salvia miltiorrhiza: bitter and slightly cold in nature, it enters the heart, pericardium and liver meridians. It has the effects of promoting blood circulation, removing blood stasis, regulating menstruation and relieving pain, relieving restlessness and calming the mind. It is mainly used to treat irregular menstruation, postpartum abdominal pain caused by blood stasis, trauma, irritability and other diseases.

[0020] Alisma: sweet, light in taste, cold in nature; enters the kidney and bladder meridians; has the effects of dispelling water and dampness, relieving heat, resolving turbidity and lowering lipids; used for dysuria, edema, diarrhea, oliguria, phlegm and fluid, dizziness, painful stranguria due to heat, and hyperlipidemia.

[0021] (3) Beneficial effects

[0022] Fresh herbs are a summary of thousands of years of experience in traditional Chinese medicine and are a hallmark of TCM diagnosis and treatment, with a long history of clinical application. Fresh herbs retain their natural activity and are characterized by abundant active ingredients, rapid efficacy, and high quality. The present invention adheres to TCM theory and adheres to the principle of syndrome differentiation and treatment to obtain a traditional Chinese medicine fresh herb composition consisting of fresh wax gourd seeds, fresh lotus leaves, fresh dianthus, fresh salvia miltiorrhiza, and fresh alisma orientalis. In the traditional Chinese medicine fresh medicine composition of the present invention, fresh wax gourd seeds are the main drug, which mainly achieves the effects of clearing heat and resolving phlegm, promoting diuresis and relieving stranguria, and is the core drug of the entire prescription; fresh lotus leaves are the ministerial drug, which assists the main drug in clearing away heat and removing dampness, raising yang and stopping bleeding, and enhancing the heat-clearing and damp-removing effects of the prescription; fresh dianthus fragrans is the adjuvant, which assists the main and ministerial drugs in clearing heart heat, promoting damp-heat in the small intestine and bladder, and further strengthening the diuretic and stranguria effects; fresh salvia miltiorrhiza is the adjuvant, which plays a role in promoting blood circulation and removing blood stasis, assisting the main and ministerial drugs in improving blood circulation, and may also help alleviate symptoms caused by damp-heat; fresh oriental rhizome also serves as an adjuvant, which plays a role in promoting diuresis, dispelling dampness, and relieving heat, and works together with the main drug, fresh wax gourd seeds, to strengthen the diuretic and stranguria effects. The whole prescription has the effects of promoting diuresis and relieving stranguria, promoting blood circulation and removing blood stasis, and dispelling dampness and relieving heat.

[0023] The fresh Chinese herbal medicine combination of the present invention has significant diuretic and anti-inflammatory effects, and its therapeutic effect is significantly superior to that of freeze-dried and processed products, comparable to that of hydroflumethiazide, and can be used as a substitute for Western medicine. Furthermore, the various components of the fresh Chinese herbal medicine combination of the present invention synergistically enhance efficacy, and none of them can be missing. The fresh Chinese herbal medicine combination of the present invention has no toxic side effects in mice and is safe and effective. The fresh Chinese herbal medicine combination of the present invention provides new ideas for the development and application of diuretics. DETAILED DESCRIPTION

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0025] Example 1

[0026] A diuretic fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 10 parts of fresh wax gourd seeds, 10 parts of fresh lotus leaves, 5 parts of fresh dianthus, 5 parts of fresh salvia miltiorrhiza, and 5 parts of fresh alisma orientalis.

[0027] In the traditional Chinese medicine fresh medicine composition, fresh wax gourd seeds are seeds of Benincasa hispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh Dianthus superbus L. is the whole herb of the perennial herb Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh Salvia miltiorrhiza is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh Alisma plantago is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alismataceae family. The above medicinal herbs are freshly picked when used and are not processed or prepared in any way.

[0028] Example 2

[0029] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh wax gourd seeds, 25 parts of fresh lotus leaves, 20 parts of fresh dianthus, 20 parts of fresh salvia miltiorrhiza, and 20 parts of fresh alisma orientalis.

[0030] Example 3

[0031] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 14 parts of fresh wax gourd seeds, 12 parts of fresh lotus leaves, 8 parts of fresh dianthus, 7 parts of fresh salvia miltiorrhiza, and 6 parts of fresh alisma orientalis.

[0032] Example 4

[0033] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 22 parts of fresh wax gourd seeds, 20 parts of fresh lotus leaves, 16 parts of fresh dianthus, 14 parts of fresh salvia miltiorrhiza, and 14 parts of fresh alisma orientalis.

[0034] Example 5

[0035] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 15 parts of fresh wax gourd seeds, 10 parts of fresh lotus leaves, 5 parts of fresh dianthus, 5 parts of fresh salvia miltiorrhiza, and 5 parts of fresh alisma orientalis.

[0036] Example 6

[0037] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh wax gourd seeds, 20 parts of fresh lotus leaves, 15 parts of fresh dianthus, 15 parts of fresh salvia miltiorrhiza, and 15 parts of fresh alisma orientalis.

[0038] Example 7

[0039] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 18 parts of fresh wax gourd seeds, 14 parts of fresh lotus leaves, 10 parts of fresh dianthus, 8 parts of fresh salvia miltiorrhiza, and 8 parts of fresh alisma orientalis.

[0040] Example 7

[0041] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 16 parts of fresh lotus leaves, 12 parts of fresh dianthus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0042] Example 8

[0043] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh dianthus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0044] Test example

[0045] Study on the acute toxicity of the diuretic and anti-inflammatory effects of the fresh Chinese medicinal composition of the present invention

[0046] 1. Materials

[0047] 1.1 Experimental drugs

[0048] Test drug 1: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh Dianthus sibiricum, 10 parts of fresh Salvia miltiorrhiza, and 10 parts of fresh Alisma orientalis.

[0049] Test drug 2: 13 portions of fresh Dianthus dianthus, 10 portions of fresh Salvia miltiorrhiza, and 10 portions of fresh Alismatis rhizome.

[0050] Test drug 3: 20 portions of fresh wax gourd seeds and 18 portions of fresh lotus leaves.

[0051] Test drug 4: freeze-dried drink of test drug 1.

[0052] Test drug 5: 20 parts of wax gourd seeds, 18 parts of lotus leaves, 13 parts of Dianthus superbus, 10 parts of Salvia miltiorrhiza, and 10 parts of Alisma orientalis.

[0053] Test drug 6: Hydrochlorothiazide tablets, Changzhou Pharmaceutical Factory Co., Ltd., 25 mg × 100 tablets.

[0054] Among the test drugs 1-3, fresh wax gourd seeds are the seeds of Benincasa hispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are the leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh superbus is the whole herb of Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh salvia is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh Alisma plantago is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alismataceae family. The above medicinal herbs were freshly picked when used and were not processed or prepared in any way.

[0055] Preparation of test drugs 1-3: Weigh each fresh medicinal material according to the above-mentioned weight proportions, place it in an ultracentrifugal grinder at 18000rm, then pass it through an 80-mesh sieve, and finally mix, squeeze and crush it with a fruit and vegetable wall breaker. Then, use purified water to prepare a 1g / mL suspension to obtain test drugs 1-3, which are stored in a 4°C medicine cool cabinet.

[0056] Preparation of test drug 4: Weigh each fresh Chinese medicinal material according to the above-mentioned weight proportions, pre-freeze at -30°C for more than 24 hours, place in a vacuum freeze dryer, set the time and pressure, set the vacuum degree to 0.2 mbar, set the temperature to -50°C, set the freeze-drying time to 48 hours, and perform freeze-drying; after taking out, crush, pass through an 80-mesh sieve, and prepare a 1 g / mL suspension with purified water to obtain test drug 4, which was stored in a 4°C medicine cool cabinet.

[0057] Preparation of test drug 5: Weigh each Chinese medicinal material according to the above-mentioned weight proportions (the Chinese medicinal materials used were purchased from Zhang Zhongjing Pharmacy, and all were processed medicines prepared in accordance with the pharmacopoeia methods and standards), wash, dry, soak in 3-4 times purified water for 30 minutes, then decoct for 40 minutes, decoct twice, combine the filtrate and concentrate to prepare a Chinese medicine stock solution with a drug concentration of 1 g / mL, and obtain test drug 5.

[0058] 2 Experimental methods

[0059] 2.1 Diuresis experiment

[0060] 2.1.1 Experimental animals

[0061] SPF grade SD rats, male, weighing 180-220g were taken. The animals were raised in an SPF grade laboratory, and after being divided into cages according to the random number table method, the breeding conditions were maintained: temperature (26±2)℃, relative humidity (49±5)%, feeding on time every day, and unlimited water supply. The Aston method was used to screen SD rats: the rats were first adaptively fed in metabolic cages for 3 days. After the adaptive feeding was completed, they were fasted but not watered for 18 hours, and gavaged with deionized water at a volume of 25mL / kg. The urine of the rats was collected two hours after the gavage. The rats whose urine mass was greater than 40% of the gavage mass were screened as qualified rats for this diuresis experiment.

[0062] 2.1.2 Experimental Grouping and Dosing

[0063] Eighty-four qualified rats were screened and randomly divided according to body weight into a blank control group (blank group), a positive control group (hydrochlorothiazide group), and experimental groups 1-5, with 12 rats in each group. The experiment used a metabolic cage method, and each group received the prescribed dose via gavage for 7 consecutive days. The dosing method is shown in Table 1.

[0064] Table 1 Drug administration methods for rats in each group

[0065] Grouping Quantity (n) drug Dosage Blank group 12 pure water Same volume Hydrochlorothiazide group 12 Hydrochlorothiazide tablets 16 mg / kg aqueous solution Experimental Group 1 12 Test drug 1 10g / kg Experimental Group 2 12 Test drug 2 10g / kg Experimental group 3 12 Test drug 3 10g / kg Experimental Group 4 12 Test drug 4 3g / kg Experimental Group 5 12 Test drug 5 3g / kg

[0066] The drug was administered continuously for 7 days. The rats were fasted but not watered for 24 hours before the last administration. Each group was gavaged with 0.9% sodium chloride solution at a dose of 25 mL / kg (which can increase the extracellular fluid of the animals and simulate the state of water and sodium retention). 20 minutes later, the drug was administered by gavage according to the dose, and the blank group was given the same volume of normal saline. After administration, the lower abdomen of the rat was gently pressed to empty the urine in the bladder. The rat was placed in a metabolic cage and urine was collected once every hour for a total of 5 times. The urine volume per hour was recorded. The last urine collection required emptying the urine in the rat's bladder. The collected urine was used to detect Na + , K + , Cl — The amount of ions discharged.

[0067] 2.1.3 Detection indicators and methods

[0068] (1) Collect and record the urine volume of rats in each group within 5 hours after the last administration.

[0069] (2) Determine the excretion of Na+, K+, and Cl- ions in urine.

[0070] 2.2 Carrageenan-induced rat toe swelling experiment

[0071] Seventy SPF male SD rats were randomly divided into 7 groups according to body weight, with 12 rats in each group. The grouping and administration methods were as shown in 2.1.2. Each group was gavage-administered once a day for 7 consecutive days. 60 minutes after the last administration, 0.1 mL of 1% carrageenan was subcutaneously injected into the right hind paw of each rat. The volume of the right hind paw of each rat was measured 1, 3, and 6 hours after injection. The degree of right hind paw swelling and the toe swelling inhibition rate of the rats in each group at different times after inflammation were compared.

[0072] Toe swelling Δm = toe volume after inflammation - toe volume before inflammation

[0073] Paw swelling inhibition rate (I%) = (average paw swelling degree of the model group - average paw swelling degree of the drug-treated group) / average paw swelling degree of the model group × 100%.

[0074] 2.3 Acute toxicity test

[0075] 2.3.1 Half-lethal experiment

[0076] Forty Kunming mice weighing (20±2) g were randomly divided into four groups, each containing 10 mice, half male and half female. Group 1 received a high-dose group (40 g / kg) of test drug 1, group 2 received a medium-dose group (20 g / kg) of test drug 1, group 3 received a low-dose group (10 g / kg), and group 4 served as the control group. The mice were fasted but not watered for one day before the test. On the second day, the test group received 0.2 mL of saline by gavage, while the control group received an equal volume of saline. Six hours after gavage, the mice were fed and observed for seven consecutive days. Deaths and median lethal doses (LD50) were recorded. If all mice in the test drug group survived and the LD50 value could not be determined, it indicated that the test drug had very low toxicity and a maximum dose test could be conducted in accordance with the Technical Guidelines for Acute Toxicity Studies of Traditional Chinese Medicines and Natural Drugs.

[0077] 2.3.2 Maximum dosage experiment

[0078] Twenty Kunming mice weighing (20±2) g were randomly divided into two groups, each with 10 mice, half male and half female. The experimental group was gavaged with the maximum dose (40 g / kg) of test drug 1 and the maximum gavage volume (40 mL / kg), while the control group was gavaged with an equal volume of normal saline. The mice were observed for 7 consecutive days after administration, and the clinical manifestations, weight changes, and mortality of the mice were observed. The clinical manifestations of the mice were recorded for 7 consecutive days after gavage. The weight of the mice was measured on days 1, 3, 5, and 7 after gavage, and mortality was observed.

[0079] 2.4 Statistical analysis

[0080] SPSS 19.0 statistical software was used for analysis, and the experimental data were expressed as mean ± standard deviation (SD). The data were compared between two groups using the one-sample t-test, and between multiple groups using the homogeneity of variance test in one-way ANOVO. P < 0.05 indicated statistical significance, and P < 0.01 indicated statistically significant differences. GraphPrism 8.0.1 software was used for plotting.

[0081] 3 Results

[0082] 3.1 Effect of the fresh Chinese medicinal composition of the present invention on urine volume in water-loaded rat models

[0083] As shown in Table 2, 1 hour after administration, the urine volume of rats in the hydrochlorothiazide group and experimental groups 1-5 increased significantly compared with the blank group, and the differences were statistically significant (p<0.05, p<0.01). The urine volume of rats in experimental group 1 was significantly higher than that in experimental groups 2-3 and experimental group 5 (p<0.05, p<0.01), but there was no statistically significant difference between the hydrochlorothiazide group and experimental group 4 (p>0.05). 3 hours and 5 hours after administration, the urine volume of rats in the hydrochlorothiazide group and experimental groups 1-5 increased significantly compared with the blank group (p<0.05, p<0.01). The urine volume of rats in experimental group 1 was significantly higher than that in experimental groups 2-5 (p<0.05, p<0.01), but there was no statistically significant difference between the hydrochlorothiazide group and the hydrochlorothiazide group (p>0.05). The above results show that the diuretic effect of the fresh Chinese medicinal composition of the present invention (test drug 1) is obvious, which is equivalent to the diuretic effect of the Western medicine hydrochlorothiazide.

[0084] Table 2 Urine volume of water-loaded rat models in each group n=12)

[0085] Group 1h(mL) 3h(mL) 5h(mL) Blank group <![CDATA[1.39±0.22 ## ]]> <![CDATA[3.48±0.34 ## ]]> <![CDATA[5.01±0.27 ## ]]> Hydrochlorothiazide group 2.31±0.43** 6.82±0.39** 9.06±0.41** Experimental Group 1 2.39±0.37** 6.88±0.33** 9.67±0.32** Experimental Group 2 <![CDATA[1.78±0.30* ## ]]> <![CDATA[4.01±0.30* ## ]]> <![CDATA[6.83±0.33** ## ]]> Experimental group 3 <![CDATA[1.88±0.31** ## ]]> <![CDATA[4.16±0.40** ## ]]> <![CDATA[6.21±0.20** ## ]]> Experimental Group 4 2.20±0.29** <![CDATA[5.59±0.34** ## ]]> <![CDATA[7.62±0.41** ## ]]> Experimental Group 5 <![CDATA[2.03±0.29** # ]]> <![CDATA[5.51±0.38** ## ]]> <![CDATA[7.52±0.34** ## ]]>

[0086] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0087] 3.2 Effect of the fresh Chinese medicinal composition of the present invention on the Na level in urine of water-loaded rat model within 5 h + , K + 、Cl － The impact of discharge

[0088] As shown in Table 3, compared with the blank group, the hydrochlorothiazide group and experimental group 1, experimental group 4 and group 5 were able to significantly increase Na + , K + 、Cl － The difference was statistically significant (p<0.05, p<0.01). + , K + 、Cl － The excretion of Na in the hydrochlorothiazide group was higher than that in the experimental groups 2-5. + , K + 、Cl － The results show that the Chinese herbal medicine fresh drug composition (test drug 1) has an advantage in promoting the excretion of electrolytes in urine, and its diuretic effect is comparable to that of the Western medicine hydrochlorothiazide.

[0089] Table 3 Na in urine of rats with water loading in each group within 5 hours + , K + 、Cl － Discharge n=12)

[0090] Group <![CDATA[Na + ( mmol / L )]]> <![CDATA[K + ( mmol / L )]]> <![CDATA[Cl － ( mmol / L )]]> Blank group <![CDATA[126.37±8.02 ## ]]> <![CDATA[3.06±1.36 # ]]> <![CDATA[127.88±8.06 ## ]]> Hydrochlorothiazide group 153.32±13.31** 15.99±2.94* 145.77±14.29** Experimental Group 1 151.57±7.79** 16.63±1.64* 142.87±12.78** Experimental Group 2 <![CDATA[130.59±14.94 ## ]]> 9.36±3.16 134.78±11.41 Experimental group 3 <![CDATA[131.49±18.21 ## ]]> 10.44±2.09 <![CDATA[132.69±10.24 # ]]> Experimental Group 4 145.82±19.96** 14.68±3.11* 139.88±11.38* Experimental Group 5 <![CDATA[139.91±12.79* # ]]> 14.35±2.48* 138.99±10.13*

[0091] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0092] 3.3 Effect of the fresh Chinese medicinal composition of the present invention on carrageenan-induced toe swelling in rats

[0093] As shown in Table 4, compared with the blank group, hydrochlorothiazide and test drugs 1-5 had a significant inhibitory effect on carrageenan-induced rat toe swelling, and the difference was statistically significant (p < 0.01). The inhibitory effect of the fresh Chinese herbal medicine composition of the present invention on carrageenan-induced rat toe swelling was significantly greater than that of test drugs 2-6 (p < 0.01), indicating that the fresh Chinese herbal medicine composition of the present invention has a good anti-inflammatory effect.

[0094] Table 4 Carrageenan-induced swelling of rat toes n=12)

[0095]

[0096] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0097] Table 5 Inhibition rate of the fresh Chinese medicinal composition of the present invention on carrageenan-induced rat toe swelling

[0098]

[0099]

[0100] 3.4 Application of the fresh Chinese herbal medicine combination of the present invention in the acute toxicity test in mice

[0101] 3.4.1 Half-lethal test

[0102] As shown in Table 6, mice were observed for 7 days after administration. All mice in each group showed no abnormalities and were in good mental state. No mortality was found. No pathological changes were found in the organs and tissues after autopsy.

[0103] Table 6 Death of mice

[0104] Group quantity Deaths Survival rate (%) control group 10 0 100 Low-dose group 10 0 100 Medium dose group 10 0 100 High-dose group 10 0 100

[0105] 3.4.2 Maximum dosage experiment

[0106] As shown in Table 7, mice were observed for 7 days after administration. All mice in each group showed no abnormalities and were in good mental state. No mortality was found. No pathological changes were found in the organs and tissues after autopsy.

[0107] As shown in Table 8, the body weight of mice in each group increased normally during the experiment, with no significant difference (P>0.05).

[0108] Table 7 Death of mice

[0109] Group quantity Deaths Survival rate (%) control group 10 0 100 experimental group 10 0 100

[0110] Table 8 Changes in mouse body weight

[0111] Group 1d 3d 5d 7d control group 21.42±2.44 23.86±0.67 24.11±0.25 24.63±0.41 experimental group 21.37±1.58 23.42±0.79 23.99±0.57 24.38±0.64

[0112] 4 Conclusion

[0113] Fresh medicine is the summary of the experience of using medicine in traditional Chinese medicine for thousands of years, is a feature of Chinese medicine diagnosis and treatment, and its clinical application has a long history. Fresh medicine retains the natural activity of medicine, has the characteristics of abundant active ingredient amount, fast efficacy, good quality, the present invention complies with the theory of traditional Chinese medicine, with the principle of treating according to syndrome differentiation, obtains the Chinese medicine fresh medicine composition consisting of fresh wax gourd seeds, fresh lotus leaves, fresh herb, fresh salvia miltiorrhiza and fresh rhizoma alismatis. According to the above experimental results, the Chinese medicine fresh medicine composition of the present invention has significant diuretic and anti-inflammatory effects, and the effect is obviously better than freeze-dried product (test drug 4) and processed product (test drug 5), suitable with hydroflumethiazide effect, can replace Western medicine use, and each component synergistically enhances synergy in the fresh medicine composition of the present invention simultaneously, and one is indispensable. The Chinese medicine fresh medicine composition of the present invention has no toxic and side effects to mice, and is safe and effective. The Chinese medicine fresh medicine composition of the present invention provides new ideas for the development and application of diuretics.

[0114] In the traditional Chinese medicine fresh medicine composition of the present invention, fresh wax gourd seeds are the main drug, which mainly achieves the effects of clearing heat and resolving phlegm, promoting diuresis and relieving stranguria, and is the core drug of the entire prescription; fresh lotus leaves are the ministerial drug, which assists the main drug in clearing away heat and removing dampness, raising yang and stopping bleeding, and enhancing the heat-clearing and damp-removing effects of the prescription; fresh dianthus fragrans is the adjuvant, which assists the main and ministerial drugs in clearing heart heat, promoting damp-heat in the small intestine and bladder, and further strengthening the diuretic and stranguria effects; fresh salvia miltiorrhiza is the adjuvant, which plays a role in promoting blood circulation and removing blood stasis, assisting the main and ministerial drugs in improving blood circulation, and may also help alleviate symptoms caused by damp-heat; fresh oriental rhizome also serves as an adjuvant, which plays a role in promoting diuresis, dispelling dampness, and relieving heat, and works together with the main drug, fresh wax gourd seeds, to strengthen the diuretic and stranguria effects. The whole prescription has the effects of promoting diuresis and relieving stranguria, promoting blood circulation and removing blood stasis, and dispelling dampness and relieving heat.

[0115] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A diuretic Chinese medicinal fresh medicine composition, characterized in that: The traditional Chinese medicine fresh medicine composition is composed of the following raw materials in parts by weight: 10-25 parts of fresh wax gourd seeds, 10-25 parts of fresh lotus leaves, 5-20 parts of fresh dianthus, 5-20 parts of fresh salvia miltiorrhiza, and 5-20 parts of fresh alisma orientalis.

2. A diuretic Chinese medicinal fresh medicine composition according to claim 1, characterized in that, The traditional Chinese medicine fresh medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wax gourd seeds, 10-20 parts of fresh lotus leaves, 5-15 parts of fresh dianthus, 5-15 parts of fresh salvia miltiorrhiza, and 5-15 parts of fresh alisma orientalis.

3. A diuretic Chinese medicinal fresh medicine composition according to claim 1, characterized in that, The traditional Chinese medicine fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh dianthus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

4. A diuretic Chinese medicinal fresh medicine composition according to any one of claims 1 to 3, characterized in that: In the Chinese medicinal fresh medicine composition, fresh wax gourd seeds are wax gourds of the genus Wax Gourd of the Cucurbitaceae family. Benincasa hispida (Thunb.) Cogn. Seeds of the Nymphaeaceae plant Nelumbo nucifera; fresh lotus leaves are Nelumbo nucifera Gaertn Fresh Dianthus is a perennial herb of the genus Dianthus in the family Caryophyllaceae. Dianthus superbus The whole herb of L.; fresh Salvia miltiorrhiza is a perennial upright herb of the genus Salvia of the family Lamiaceae. Salvia miltiorrhiza Bunge The root of fresh Alisma orientalis is a perennial aquatic or marsh herb of the genus Alisma of the family Alismataceae. Alisma plantago-aquatica The stem of L.; the herb is freshly picked when used without any processing or preparation.

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