Microbial agent culture equipment

Abstract

The microbial agent culture equipment comprises a culture tank, the upper end of the culture tank is of an opening structure, a first motor is arranged in the middle of the outer side of the lower end of the culture tank, and a driving shaft of the first motor extends into the culture tank and is fixedly connected with the lower end of a first rotating shaft; the upper end of the first rotating shaft is fixedly connected with the middle of the bottom of a first rotating disc, a plurality of through holes are evenly formed in the first rotating disc in the circumferential direction, and a culture dish is arranged in each through hole; a tank cover matched with the culture tank is arranged on the upper portion of the culture tank, a dish taking opening is formed in one end of the tank cover, a second rotating disc is rotationally arranged on the portion, on the outer side of the dish taking opening, of the tank cover, and a second motor used for driving the second rotating disc to rotate is arranged on the culture tank. The contact area between the interior of the culture tank and external air is effectively reduced, so that the contact opportunity between impurities in the air and the culture dish is reduced, and the culture safety of a microbial agent is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial agent cultivation equipment, and in particular to a microbial agent cultivation equipment. Background Art

[0002] Microbial agents refer to live bacterial preparations made by using porous substances as adsorbents to adsorb the fermentation broth of target microorganisms after industrial production and expansion.

[0003] At present, the cultivation of microbial agents needs to be carried out in a directional manner in a culture dish of the corresponding culture equipment. When adding nutrient solution to the culture dish, the existing microbial culture equipment needs to open the door of the entire incubator. The wide opening of the door will cause impurities in the air to quickly enter the incubator, causing pollution to the internal environment of the incubator. In addition, the nutrient solution used for the cultivation of microbial agents is generally prepared from a variety of elements. After being placed for a period of time, the internal elements of the nutrient solution will precipitate, causing various elements to accumulate at the bottom of the nutrient solution. The microbial agents above the nutrient solution cannot absorb sufficient nutrients, which will seriously inhibit the growth of the microbial agents. Therefore, a cell culture device for cell therapy is developed. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial inoculant culture device to solve the technical problems mentioned in the above background technology.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] The present invention provides a microbial inoculant cultivation device, comprising a cultivation tank, wherein the upper end of the cultivation tank is an open structure, and a first motor is arranged in the middle of the outer side of the lower end, and a driving shaft of the first motor extends into the interior of the cultivation tank and is fixedly connected to the lower end of the first rotating shaft, and the upper end of the first rotating shaft is fixedly connected to the middle of the bottom of a first rotating disk, and a plurality of through holes are evenly arranged on the first rotating disk along the circumferential direction, and a cultivation dish is arranged in each of the through holes; a tank cover adapted to the cultivation tank is arranged on the upper part, and a dish taking opening is arranged at one end of the tank cover, and a second rotating disk is rotatably arranged on the outer side of the dish taking opening on the tank cover, and a second motor for driving the second rotating disk to rotate is arranged on the cultivation tank; the upper end of the second rotating disk A mounting frame is fixedly provided on the top, a fixed platform is fixedly provided on the middle part of one side of the mounting frame, a first lifting cylinder is vertically provided on the end of the fixed platform away from the mounting frame, and a sealing cover matched with the dish taking port is fixedly provided on the telescopic end of the first lifting cylinder; a lifting platform which can reciprocate in the vertical direction is provided on the other side of the mounting frame, and a driving mechanism for driving the lifting platform to move linearly is also provided on the mounting frame; a clamping mechanism for clamping the culture dish is provided on the side of the lifting platform away from the mounting frame, and an inverted U-shaped mounting frame is fixedly provided on the end of the tank cover opposite to the mounting frame, and a stirring mechanism for stirring the nutrient base liquid inside the culture dish is provided on the mounting frame.

[0007] Furthermore, two pairs of supporting legs are symmetrically fixedly arranged at the bottom of the culture tank near the outer edge.

[0008] Furthermore, an annular heater is fixedly arranged on the bottom plate of the culture tank below the plurality of culture dishes.

[0009] Furthermore, a heat-insulating layer is fixedly provided on the inner peripheral wall of the culture tank.

[0010] Furthermore, a plurality of threaded holes which are opposite to each other are evenly formed on the upper end of the peripheral wall of the culture tank and the tank cover along the circumferential direction, and the tank cover is fixedly connected to the culture tank by a plurality of screws which are matched with the threaded holes.

[0011] Furthermore, a second rotating shaft rotatably connected to the tank cover is fixedly arranged at the bottom of the second turntable, and the second motor is fixedly arranged outside the culture tank in the horizontal direction, and its driving shaft extends to the inside of the culture tank and is transmission-connected to the lower end of the second rotating shaft.

[0012] Furthermore, a driving bevel gear is provided at the free end of the driving shaft of the second motor, a driven bevel gear matched with the driving bevel gear is provided at the lower end of the second rotating shaft, and a protective box is fixedly provided on the inner wall of the culture tank on the outer sides of the driving bevel gear and the driven bevel gear.

[0013] Furthermore, the driving mechanism includes a screw rod rotatably arranged in the middle of the mounting frame, a third motor for driving the screw rod to rotate is arranged on the upper part of the mounting frame, and two sliding rods are symmetrically fixedly arranged on the mounting frame along the screw rod; a threaded sleeve threadedly connected to the screw rod is fixedly arranged in the middle of one end of the lifting platform close to the mounting frame, and two sliding sleeves respectively slidingly matched with the two sliding rods are fixedly arranged.

[0014] Furthermore, the clamping mechanism includes a two-way cylinder fixedly arranged on the upper part of the lifting platform, and the lifting platform is provided with sliding grooves at both ends of the two-way cylinder. The two telescopic ends of the two-way cylinder are respectively fixed with connecting rods that slide with the sliding grooves, and the lower ends of the two connecting rods are relatively fixed with two clamping claws.

[0015] Furthermore, the stirring mechanism includes a fourth motor fixedly arranged in the middle of the upper part of the mounting frame and two second lifting cylinders symmetrically arranged on both sides of the fourth motor, the driving shaft of the fourth motor is fixedly connected to the transmission shaft, the outer sliding sleeve of the transmission shaft is provided with a stirring shaft sleeve, a plurality of connecting ridges are evenly fixedly arranged on the outer peripheral wall of the transmission shaft along the circumferential direction, and a plurality of connecting grooves that are matched one by one with each of the connecting ridges are evenly opened on the inner peripheral wall of the stirring shaft sleeve; the telescopic ends of the two second lifting cylinders are commonly fixedly connected to the upper parts of the two ends of the connecting plate, the part of the stirring shaft sleeve close to the upper end is rotatably matched with the middle part of the connecting plate, and limit plates are fixedly arranged at the parts located on the upper and lower sides of the connecting plate respectively; the stirring shaft sleeve is slidably matched with the feed hole opened on the tank cover, and a plurality of stirring blades are evenly fixedly arranged on the lower end of the stirring shaft sleeve along the circumferential direction.

[0016] Compared with the prior art, the beneficial technical effects of the present invention are:

[0017] The present invention effectively reduces the opening area of ​​the culture tank when adding culture solution to the culture dish and taking and placing the culture dish, thereby reducing the contact area between the inside of the culture tank and the outside air and further reducing the contact opportunity between impurities in the air and the culture dish, which is beneficial to ensure the safety of microbial inoculant cultivation. In addition, the present invention can realize the automatic stirring operation of the nutrient solution in each culture dish in turn, avoiding the situation where the elements inside the nutrient solution are precipitated and accumulated at the bottom of the culture dish for a long time, and ensuring the normal growth of the microbial inoculant. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described below in conjunction with the accompanying drawings.

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2It is a cross-sectional structural schematic diagram of the present invention;

[0021] Figure 3 It is a schematic diagram of the structure of the incubator of the present invention;

[0022] Figure 4 It is a schematic diagram of the can cover structure of the present invention;

[0023] Figure 5 It is a cross-sectional schematic diagram of the connection portion between the transmission shaft and the stirring shaft of the present invention;

[0024] Explanation of the reference numerals: 1. culture tank; 2. first motor; 3. support leg; 4. first rotating shaft; 5. first turntable; 6. culture dish; 7. limiting convex ring; 8. annular heater; 9. insulation layer; 10. tank cover; 11. screw; 12. dish taking opening; 13. second turntable; 14. second motor; 15. second rotating shaft; 16. active bevel gear; 17. driven bevel gear; 18. protective box; 19. mounting frame; 20. fixing table; 21. first lifting cylinder ; 22. Sealing cover; 23. Lifting platform; 24. Screw; 25. Third motor; 26. Slide rod; 27. Threaded sleeve; 28. Slide sleeve; 29. ​​Bidirectional cylinder; 30. Slide groove; 31. Connecting rod; 32. Clamp; 33. Mounting frame; 34. Fourth motor; 35. Second lifting cylinder; 36. Transmission shaft; 37. Stirring shaft sleeve; 38. Connecting convex strip; 39. Connecting groove; 40. Connecting plate; 41. Limiting plate; 42. Feed hole; 43. Stirring blade. DETAILED DESCRIPTION

[0025] like Figure 1 As shown, a microbial inoculant cultivation device includes a culture tank 1, the upper end of the culture tank 1 is an open structure, and a first motor 2 is fixedly installed in the middle of the outer side of the lower end along the vertical direction. In addition, two pairs of supporting legs 3 are symmetrically fixedly installed at the bottom of the culture tank 1 near the outer edge.

[0026] The driving shaft of the first motor 2 extends to the interior of the culture tank 1 and is fixedly connected to the lower end of the first rotating shaft 4. The upper end of the first rotating shaft 4 is fixedly connected to the middle of the bottom of the first turntable 5. The first turntable 5 is evenly provided with a plurality of through holes along the circumferential direction, and a culture dish 6 is inserted in each of the through holes. A limiting convex ring 7 with a diameter larger than the inner diameter of the through hole is fixedly provided on the outer periphery of the culture dish 6 near the upper end.

[0027] An annular heater 8 is fixedly installed on the bottom plate of the culture tank 1 below the plurality of culture dishes 6 , and an insulation layer 9 is fixedly installed on the inner peripheral wall of the culture tank 1 .

[0028] The upper part of the culture tank 1 is equipped with a tank cover 10 that matches it. Specifically, a plurality of threaded holes that are evenly arranged one by one and opposite to each other are formed on the upper end of the peripheral wall of the culture tank 1 and the tank cover 10 along the circumferential direction. The tank cover 10 is fixedly connected to the culture tank 1 by a plurality of screws 11 that match the threaded holes.

[0029] One end of the tank cover 10 is provided with a dish taking opening 12, and a second turntable 13 is rotatably mounted on the tank cover 10 at the outer side of the dish taking opening 12, and a second motor 14 for driving the second turntable 13 to rotate is mounted on the culture tank 1. Specifically: In this embodiment, a second rotating shaft 15 rotatably connected to the tank cover 10 is fixedly mounted at the bottom of the second turntable 13, and the second motor 14 is fixedly mounted on the outside of the culture tank 1 in a horizontal direction, and its driving shaft extends to the inside of the culture tank 1 and is transmission-connected to the lower end of the second rotating shaft 15. Specifically: A driving bevel gear 16 is mounted on the free end of the driving shaft of the second motor 14, and a driven bevel gear 17 matched with the driving bevel gear 16 is mounted on the lower end of the second rotating shaft 15, and a protective box 18 is fixedly mounted on the inner wall of the culture tank 1 on the outer sides of the driving bevel gear 16 and the driven bevel gear 17.

[0030] A mounting frame 19 is fixedly mounted on the upper part of the second turntable 13, a horizontal fixed platform 20 is fixedly mounted in the middle of one side of the mounting frame 19, a first lifting cylinder 21 is vertically mounted on one end of the fixed platform 20 away from the mounting frame 19, and a sealing cover 22 adapted to the dish taking port 12 is fixedly mounted on the telescopic end of the first lifting cylinder 21. A lifting platform 23 capable of reciprocating in the vertical direction is mounted on the other side of the mounting frame 19, and a driving mechanism for driving the lifting platform 23 to move linearly is also mounted on the mounting frame 19.

[0031] In this embodiment, the driving mechanism includes a screw rod 24 rotatably mounted in the middle of the mounting frame 19, a third motor 25 for driving the screw rod 24 to rotate is mounted on the upper portion of the mounting frame 19, and two slide rods 26 are symmetrically fixedly mounted on the mounting frame 19 along the screw rod 24. A threaded sleeve 27 threadedly connected to the screw rod 24 is fixedly disposed in the middle of one end of the lifting platform 23 close to the mounting frame 19, and two slide sleeves 28 respectively slidably matched with the two slide rods 26 are fixedly disposed.

[0032] A clamping mechanism for clamping the culture dish 6 is provided on the side of the lifting platform 23 away from the mounting frame 19. In the present embodiment, the clamping mechanism comprises a bidirectional cylinder 29 horizontally fixedly mounted on the upper part of the lifting platform 23. The lifting platform 23 is provided with slide grooves 30 at both ends of the bidirectional cylinder 29. Connecting rods 31 slidably matched with the slide grooves 30 are fixedly mounted on the two telescopic ends of the bidirectional cylinder 29 respectively. Two clamping claws 32 are fixedly mounted opposite to each other at the lower ends of the two connecting rods 31.

[0033] The tank cover 10 is fixedly mounted with an inverted U-shaped mounting frame 33 at one end opposite to the mounting frame 19, and the mounting frame 33 is provided with a stirring mechanism for stirring the nutrient base liquid inside the culture dish 6. In this embodiment, the stirring mechanism includes a fourth motor 34 fixedly mounted in the middle of the upper part of the mounting frame 33 and two second lifting cylinders 35 symmetrically mounted on both sides of the fourth motor 34, the driving shaft of the fourth motor 34 is fixedly connected to the transmission shaft 36, and the outer sliding sleeve of the transmission shaft 36 is provided with a stirring shaft sleeve 37. Specifically: a plurality of connecting convex strips 38 are uniformly fixedly arranged on the outer peripheral wall of the transmission shaft 36 along the circumferential direction, and a plurality of connecting grooves 39 that are matched with each of the connecting convex strips 38 are uniformly opened on the inner peripheral wall of the stirring shaft sleeve 37. The telescopic ends of the two second lifting cylinders 35 are fixedly connected to the upper parts of the two ends of the connecting plate 40, the part near the upper end of the stirring sleeve 37 is rotatably matched with the middle part of the connecting plate 40, and the limit plates 41 are fixedly arranged at the upper and lower sides of the connecting plate 40. The stirring sleeve 37 is slidably matched with the feed hole 42 opened on the tank cover 10, and the lower end of the stirring sleeve 37 is evenly fixedly installed with a plurality of stirring blades 43 along the circumferential direction.

[0034] The present invention is specifically operated by placing each of the culture dishes on the first turntable. When it is necessary to add business liquid to the culture dish, the sealing cover is mainly moved upward by contracting the first lifting cylinder to open the dish taking port. The first motor drives the first turntable to rotate, and after the corresponding culture dish is aligned with the dish taking port, the nutrient solution can be added to the culture dish in a titration manner. When it is necessary to take out the culture dish, the turntable is driven to rotate by the second motor to make the clamping mechanism on the mounting frame align with the dish taking port, and the screw is driven to rotate by the third motor to make the lifting platform move downward. After the two clamps on the two connecting rods enter the culture tank and approach the upper end of the corresponding culture dish, the two-way cylinder contracts to make the two clamps move relative to each other to clamp the culture dish, and the third motor drives the screw to rotate in the opposite direction, and the lifting platform moves upward to take the culture dish out of the culture tank. In the above manner, the present invention effectively reduces the opening area of ​​the culture tank when adding culture liquid to the culture dish and taking and placing the culture dish, thereby reducing the contact area between the inside of the culture tank and the outside air and thus reducing the contact opportunity between impurities in the air and the culture dish, which is conducive to ensuring the safety of microbial inoculant culture.

[0035] In addition, the present invention can realize the automatic stirring operation of the nutrient solution in each culture dish in turn. When the first motor drives the corresponding culture dish to align with the stirring shaft sleeve, the two second lifting cylinders drive the connecting plate and the stirring shaft sleeve to move downward until the stirring blades at the lower end of the stirring shaft sleeve enter the interior of the culture dish. Then the fourth motor drives the transmission shaft to rotate, and the transmission shaft drives the stirring shaft sleeve and the stirring blades to rotate to realize automatic stirring of the nutrient solution in the culture dish, thereby avoiding the situation where the internal elements of the nutrient solution are precipitated and accumulated at the bottom of the culture dish for a long time, thereby ensuring the normal growth of the microbial inoculant.

[0036] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A microbial agent cultivation device, characterized in that: The invention comprises a culture tank, wherein the upper end of the culture tank is an open structure, and a first motor is arranged in the middle of the outer side of the lower end, the driving shaft of the first motor extends into the interior of the culture tank and is fixedly connected to the lower end of the first rotating shaft, the upper end of the first rotating shaft is fixedly connected to the middle of the bottom of the first rotating disk, a plurality of through holes are evenly arranged on the first rotating disk along the circumferential direction, and a culture dish is arranged in each of the through holes; a tank cover adapted to the culture tank is arranged on the upper part, a dish taking opening is arranged at one end of the tank cover, a second rotating disk is rotatably arranged on the outer side of the dish taking opening on the tank cover, and a second motor for driving the second rotating disk to rotate is arranged on the culture tank; a mounting plate is fixedly arranged on the upper part of the second rotating disk A mounting frame, a fixed platform is fixedly provided in the middle of one side of the mounting frame, a first lifting cylinder is vertically provided at one end of the fixed platform away from the mounting frame, and a sealing cover matched with the dish taking opening is fixedly provided at the telescopic end of the first lifting cylinder; a lifting platform that can reciprocate in the vertical direction is provided on the other side of the mounting frame, and a driving mechanism for driving the lifting platform to move linearly is also provided on the mounting frame; a clamping mechanism for clamping the culture dish is provided on the side of the lifting platform away from the mounting frame, and an inverted U-shaped mounting frame is fixedly provided on the end of the tank cover opposite to the mounting frame, and a stirring mechanism for stirring the nutrient base liquid inside the culture dish is provided on the mounting frame.

2. The microbial agent cultivation equipment according to claim 1, characterized in that: Two pairs of supporting legs are symmetrically fixedly arranged at the bottom of the culture tank near the outer edge.

3. The microbial agent cultivation equipment according to claim 1, characterized in that: An annular heater is fixedly arranged on the bottom plate of the culture tank below the plurality of culture dishes.

4. The microbial agent cultivation equipment according to claim 3, characterized in that: A heat preservation layer is fixedly arranged on the inner peripheral wall of the culture tank.

5. The microbial agent cultivation equipment according to claim 1, characterized in that: A plurality of threaded holes which are opposite to each other are evenly arranged on the upper end of the peripheral wall of the culture tank and the tank cover along the circumferential direction, and the tank cover is fixedly connected to the culture tank by a plurality of screws which are matched with the threaded holes.

6. The microbial agent cultivation equipment according to claim 1, characterized in that: A second rotating shaft rotatably connected to the tank cover is fixedly arranged at the bottom of the second turntable. The second motor is fixedly arranged outside the culture tank in a horizontal direction, and its driving shaft extends into the interior of the culture tank and is drivingly connected to the lower end of the second rotating shaft.

7. The microbial agent cultivation equipment according to claim 6, characterized in that: A driving bevel gear is arranged at the free end of the driving shaft of the second motor, a driven bevel gear matched with the driving bevel gear is arranged at the lower end of the second rotating shaft, and a protective box is fixedly arranged on the inner wall of the culture tank on the outer sides of the driving bevel gear and the driven bevel gear.

8. The microbial agent cultivation equipment according to claim 1, characterized in that: The driving mechanism includes a screw rod rotatably arranged in the middle of the installation frame, a third motor for driving the screw rod to rotate is arranged on the upper part of the installation frame, and two sliding rods are symmetrically fixedly arranged on the installation frame along the screw rod; a threaded sleeve threadedly connected to the screw rod is fixedly arranged in the middle of one end of the lifting platform close to the installation frame, and two sliding sleeves respectively slidingly matched with the two sliding rods are fixedly arranged.

9. The microbial agent cultivation equipment according to claim 1, characterized in that: The clamping mechanism includes a two-way cylinder fixedly arranged on the upper part of the lifting platform, and the lifting platform is provided with sliding grooves at both ends of the two-way cylinder. The two telescopic ends of the two-way cylinder are respectively fixed with connecting rods that slide in cooperation with the sliding grooves, and the lower ends of the two connecting rods are relatively fixed with two clamping claws.

10. The microbial agent cultivation equipment according to claim 1, characterized in that: The stirring mechanism includes a fourth motor fixedly arranged in the middle of the upper part of the mounting frame and two second lifting cylinders symmetrically arranged on both sides of the fourth motor, the driving shaft of the fourth motor is fixedly connected to the transmission shaft, the outer sliding sleeve of the transmission shaft is provided with a stirring shaft sleeve, a plurality of connecting convex strips are evenly fixedly arranged on the outer peripheral wall of the transmission shaft along the circumferential direction, and a plurality of connecting grooves that are matched one by one with each of the connecting convex strips are evenly opened on the inner peripheral wall of the stirring shaft sleeve; the telescopic ends of the two second lifting cylinders are commonly fixedly connected to the upper parts of the two ends of the connecting plate, the part of the stirring shaft sleeve close to the upper end is rotatably matched with the middle part of the connecting plate, and limit disks are fixedly arranged at the parts located on the upper and lower sides of the connecting plate respectively; the stirring shaft sleeve is slidably matched with the feed hole opened on the tank cover, and a plurality of stirring blades are evenly fixedly arranged on the lower end of the stirring shaft sleeve along the circumferential direction.

Images