Culture instrument for microbial agent

Abstract

The invention discloses a culture instrument of a microbial agent, and relates to the technical field of microbial agent culture, the culture instrument comprises a culture box, a rotating disc and a culture base, and a microbial filtering assembly is arranged above the rotating disc; through the arrangement of the quantitative rotating assembly and the culture medium lifting assembly, a culture medium can be fixed to the bottom of the push ring, the height difference is small when a filter membrane falls down from a fixed pipe, impact generated when the filter membrane makes contact with the culture medium due to the too large height difference is avoided, and therefore microorganisms on the filter membrane are prevented from splashing in the impact process; when a nutrient solution needs to be added, a specified culture medium is lifted to the bottom of the fixing pipe, and an operator can stretch a needle cylinder into the fixing pipe and squeeze the nutrient solution into the culture medium, so that the nutrient solution can be added into the culture medium under the condition that the incubator is not opened; and the risk that the culture medium is polluted due to air flow when the culture box is opened is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial agent cultivation, and in particular to a microbial agent cultivation instrument. Background Art

[0002] Before culturing microbial inoculants, the microorganisms need to be extracted and inoculated into the culture medium. During this process, a limit meter is required. After use, the user transfers the filter membrane into the culture medium.

[0003] During the operation of inoculating microorganisms, when using tools to transfer the filter membrane, microorganisms in the surrounding air may settle on the filter membrane or culture medium, causing contamination. Even in a relatively clean environment, such as the air conditioning wind in an ordinary laboratory, the airflow caused by the movement of people, etc., may carry tiny particles and microorganisms, increasing the risk of contamination, resulting in the failure of the final cultivation of microbial agents, or unsatisfactory cultivation effects. After the inoculation is completed, nutrient solution needs to be regularly added to the culture medium with microbial agents to meet the growth needs of the microbial agents. However, it is difficult to ensure that the nutrient solution is evenly covered in the culture medium when it is added to the culture medium. Therefore, an oscillating instrument is required to mix the microbial agents and the nutrient solution. In the process of taking the culture medium out of the culture instrument and oscillating it, the air flow is driven when the culture instrument is opened, which increases the risk of impurities in the air entering the culture medium and causing contamination. In addition, some microorganisms may die due to long-term temperature changes, which has an adverse effect on the cultivation effect of the microbial agents.

[0004] In order to solve the above problems, the inventor proposed a culture instrument for microbial inoculants. Summary of the invention

[0005] In order to solve the above technical problems, a microbial inoculant culture instrument is provided.

[0006] To achieve the above objectives, the present invention can be implemented by the following technical solutions:

[0007] The present invention provides a culture instrument for microbial inoculants, comprising: a culture box, a turntable and a culture medium seat, wherein the turntable is arranged in the culture box, the culture medium seat is fixedly connected to the top of the turntable, and a microbial filtration component is arranged above the turntable;

[0008] The microbial filtration component includes a fixed tube fixedly connected to the incubator, a threaded tube fixedly connected to the top of the fixed tube, a fixed frame fixedly connected to the threaded tube, no less than two fixed frames, two of the fixed frames are symmetrically arranged on the fixed tube, sliders are slidably connected in the two fixed frames, springs are arranged in the two fixed frames, baffles are fixedly connected to the bottoms of the two sliders respectively, liquid outlet holes are opened on the two baffles, push blocks are fixedly connected to the bottoms of the two baffles, a fixed rod is fixedly connected to the top of the turntable, the fixed rods are symmetrically arranged on both sides of each culture medium seat, and a push ring is fixedly connected to the top of the fixed rod.

[0009] Preferably, a bottle seat is fixedly connected to the top of the turntable, a liquid collecting bottle is arranged in the bottle seat, and the culture medium seat and the bottle seat are equidistantly arranged on the top of the turntable.

[0010] Preferably, the push ring has the same height as the push block, the two push blocks are V-shaped, and the push ring and the push blocks form an extrusion fit.

[0011] Preferably, a quantitative rotating assembly is provided at the bottom of the turntable, and the quantitative rotating assembly includes a solid barrel rotatably connected to the bottom of the incubator, a hexagonal rod is slidably connected in the solid barrel, the top of the hexagonal rod is fixedly connected to the turntable, a rotating circle is fixedly connected to the side of the solid barrel, a plurality of fixed teeth are annularly and equidistantly fixedly connected to the top of the rotating circle, a motor is fixedly installed on one side of the interior of the incubator, a disc is sleeved on the output shaft of the motor, a rotating rod is rotatably connected to the bottom of the disc, a fixing frame is fixedly installed on the inner bottom of the incubator, a reciprocating block is slidably connected in the fixing frame, an end of the rotating rod away from the disc is rotatably connected to the reciprocating block, and two paddles are fixedly connected to the bottom of the reciprocating block.

[0012] Preferably, the fixed teeth are arranged at equal distances.

[0013] Preferably, the two paddles are staggeredly arranged at the bottom of the reciprocating block, and a slope is arranged on one side of the two paddles close to the fixed teeth, and the two paddles form a toggle fit with the fixed teeth through the slope.

[0014] Preferably, a culture medium lifting assembly is provided at the bottom of the turntable, and the culture medium lifting assembly includes a pulley 1 mounted on the output shaft of the motor, a pulley 2 is rotatably connected in the incubator, the pulley 2 is located on the side away from the pulley 1, a belt is mounted on the pulley 1 and the pulley 2, a reciprocating screw rod is coaxially fixedly connected to the top of the pulley 2, a lifting seat is threadedly connected to the reciprocating screw rod, a shift rod is fixedly connected to the side of the lifting seat close to the turntable, a shifting groove is provided on the turntable, a limiting block is fixedly connected to the side of the lifting seat away from the shift rod, a limiting groove is provided in the incubator, the limiting groove is located on the side of the incubator close to the limiting block, and the limiting block is slidably connected in the limiting groove.

[0015] Preferably, the toggle grooves are arranged equidistantly, the number of the toggle grooves is the same as that of the turntable and the culture medium seat, the shape of the toggle groove is the same as that of the toggle rod, and the toggle rod forms a sliding fit with the toggle groove.

[0016] Preferably, a culture medium oscillation assembly is arranged under the turntable, and the culture medium oscillation assembly includes a mounting rod fixedly connected to the incubator, and the end of the mounting rod away from the incubator is fixedly connected to a wave plate, and the bottom of the turntable is fixedly connected to two connecting rods, and the two connecting rods are symmetrically arranged at the bottom of the turntable, and rollers are arranged at the bottom of the two connecting rods, and a second spring is arranged in the solid cylinder.

[0017] Preferably, the wave plate is ring-shaped, and the roller forms a rolling fit with the wave plate.

[0018] As described above, the characteristics and advantages of the culture instrument for microbial inoculants in the present invention are:

[0019] By setting the quantitative rotating component and the culture medium lifting component, the culture medium can be driven to rise until the culture medium contacts the push ring and is fixed at the bottom of the push ring. When the culture medium is at the bottom of the push ring, the height difference when the filter membrane falls from the fixed tube is small, and the filter membrane will not be impacted when it contacts the culture medium due to the excessive height difference, thereby preventing the microorganisms on the filter membrane from splashing during the impact, and preventing the filter membrane from wrinkling during the impact. At the same time, the filter membrane with bacteria is directly transferred to the culture medium under the action of gravity, and the operator does not need to use tools to manually transfer the filter membrane to the culture medium, thereby simplifying the microbial filtration process while ensuring the standardization of the operation of placing the filter membrane into the culture medium. At the same time, when nutrient solution needs to be added, the specified culture medium can be raised to the bottom of the fixed tube. At this time, the baffle is opened under the action of the push plate, and the operator can extend the syringe into the fixed tube and squeeze the nutrient solution into the culture medium, so that nutrient solution can be added to the culture medium without opening the incubator, avoiding the risk of contamination caused by air flow when the incubator is opened.

[0020] By setting the quantitative rotating assembly, two dislocated paddles successively push the fixed teeth through the inclined plane, so that the fixed teeth drive the rotating circle to rotate under the cooperation of the inclined plane, thereby driving the turntable to rotate intermittently and at a small distance during the continuous rotation of the disc, so that the culture medium and the liquid collection bottle can be accurately rotated to the bottom of the fixed tube, so as to accurately control the container at the bottom of the fixed tube, and then achieve the effect of automatic switching during the use of the device, which is convenient for use and ensures the accuracy of the operation of inserting the strain, and also avoids the problem of confusion when injecting the solution due to multiple culture media and liquid collection bottles during manual operation, thereby effectively classifying different strains;

[0021] Through the setting of the microbial filtration component, when the push ring rotates accurately and quickly to the bottom of the fixed tube, the two baffles are fully opened, and the filter membrane that loses its limit falls into the culture medium, thereby automatically completing the operation of placing the filter membrane into the culture medium. There is no need to manually transfer the filter membrane. A flamethrower is used to sterilize the inside of the fixed tube in the interval between different culture media, so as to achieve the effect of guiding users to perform bacterial filtration operations in accordance with specifications, improve the convenience of use, and avoid the occurrence of irregular operations, which is beneficial to the effect of microbial inoculant cultivation, and automatically falls into the filter membrane, avoiding the problem of cross-infection of other contaminated bacteria when picking up manually;

[0022] Through the setting of the wave plate, when the roller rolls upward on the wave plate, the two rollers prop up the turntable upward, so that the turntable pulls spring 2 through the hexagonal rod, so that spring 2 stretches, and when the roller rolls downward on the wave plate, the turntable moves downward under the action of its own gravity and the tightening of spring 2, so that the turntable continuously moves up and down during the rotation process, thereby achieving an oscillation effect, and then after the nutrient solution is added to the culture medium, the nutrient solution and the microorganisms are evenly mixed, thereby preventing the growth of microorganisms from being affected by uneven flow of the nutrient solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional schematic diagram of the overall structure shown in the present invention;

[0024] Figure 2 It is a three-dimensional schematic diagram of the overall structure shown in the present invention;

[0025] Figure 3 It is a sectional stereoscopic schematic diagram of the overall structure shown in the present invention;

[0026] Figure 4 It is a three-dimensional schematic diagram of the turntable structure shown in the present invention;

[0027] Figure 5 It is a three-dimensional schematic diagram of the fixed pipe structure shown in the present invention;

[0028] Figure 6It is a bottom-up stereoscopic schematic diagram of the fixed pipe structure shown in the present invention;

[0029] Figure 7 It is a three-dimensional schematic diagram of the structure of the culture medium lifting assembly shown in the present invention;

[0030] Figure 8 It is a bottom-up stereoscopic schematic diagram of the reciprocating block structure shown in the present invention;

[0031] Fig. 9 It is a cross-sectional perspective schematic diagram of the corrugated plate structure shown in the present invention;

[0032] Fig.10 It is a three-dimensional schematic diagram of the corrugated plate structure shown in the present invention;

[0033] Fig.11 It is a cross-sectional perspective schematic diagram of the internal structure of the solid cylinder shown in the present invention;

[0034] Fig.12 It is a three-dimensional schematic diagram of the corrugated plate structure shown in the present invention.

[0035] The reference numerals in the drawings of the present invention are: 1. incubator; 2. turntable; 3. culture medium base; 4. bottle base; 5. liquid collection bottle;

[0036] Microbial filtration assembly: 601, fixed tube; 602, threaded tube; 603, fixed frame; 604, slider; 605, spring 1; 606, baffle; 607, liquid outlet; 608, push block; 609, fixed rod; 610, push ring;

[0037] Quantitative rotating assembly: 701, solid cylinder; 702, hexagonal rod; 703, rotating circle; 704, fixed teeth; 705, motor; 706, disc; 707, rotating rod; 708, fixed frame; 709, reciprocating block; 710, paddle;

[0038] Culture medium lifting assembly: 801, pulley 1; 802, pulley 2; 803, reciprocating screw; 804, lifting seat; 805, lever; 806, toggle slot; 807, limit block; 808, limit slot;

[0039] Culture medium oscillation assembly: 901, mounting rod; 902, wave plate; 903, connecting rod; 904, roller; 905, spring 2. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0041] See 1~ Fig.12 As shown, it is an embodiment provided by the present invention, and a culture instrument for microbial inoculants provided will be described in detail below:

[0042] A culture instrument for microbial inoculants, such as Figure 1 to Figure 6 As shown, it includes: an incubator 1, a turntable 2 and a culture medium seat 3, the size of the culture medium seat 3 is the same as the culture medium, so that the culture medium can be inserted into the culture medium seat 3, the turntable 2 is arranged in the incubator 1, the culture medium seat 3 is fixedly connected to the top of the turntable 2 at an equidistant manner, and a microbial filtration component is arranged above the turntable 2;

[0043] The microbial filtration component includes a fixed tube 601 fixedly connected to the incubator 1, the fixed tube 601 is located on the movement trajectory of the culture medium seat 3, the top of the fixed tube 601 is fixedly connected to a threaded tube 602, the threaded tube 602 is used to install a funnel for introducing liquid, the threaded tube 602 is fixedly connected to a fixed frame 603, there are no less than two fixed frames 603, two fixed frames 603 are symmetrically arranged on the fixed tube 601, sliders 604 are slidably connected in the two fixed frames 603, springs 1 605 are arranged in the two fixed frames 603, both ends of the spring 1 605 are fixedly connected to the slider 604 and the fixed frame 603 respectively, the bottoms of the two sliders 604 are fixedly connected to baffles 606 respectively, and the baffles 606 located on the two sliders 604 can form a complete circle, and the baffles 606 are arranged on the baffles A sealing ring is provided which fits with the fixed tube 601, liquid outlet holes 607 are opened on the two baffles 606, push blocks 608 are fixedly connected to the bottom of the two baffles 606, a fixed rod 609 is fixedly connected to the top of the turntable 2, the fixed rods 609 are symmetrically arranged on both sides of each culture medium seat 3, a push ring 610 is fixedly connected to the top of the fixed rod 609, and the push ring 610 is located directly above the culture medium seat 3, a bottle seat 4 is fixedly connected to the top of the turntable 2, and the bottle seat 4 is respectively located between every two culture medium seats 3, the bottle seats 4 are arranged equidistantly in a ring on the turntable 2, a liquid collecting bottle 5 is arranged in the bottle seat 4, the culture medium seats 3 and the bottle seats 4 are equidistantly arranged on the top of the turntable 2, the push ring 610 has the same height as the push block 608, the two push blocks 608 are V-shaped, and the push ring 610 and the push block 608 form an extrusion fit.

[0044] Further, such as Figure 4 , Figure 7 to Figure 12As shown, a quantitative rotating assembly is provided at the bottom of the turntable 2, and the quantitative rotating assembly includes a solid barrel 701 rotatably connected to the bottom of the incubator 1, the solid barrel 701 is located at the center of the turntable 2, a hexagonal rod 702 is slidably connected in the solid barrel 701, a hole is provided on the upper part of the solid barrel 701 to match the hexagonal rod 702, the top of the hexagonal rod 702 is fixedly connected to the turntable 2, a rotating circle 703 is fixedly connected to the side of the solid barrel 701, and the center points of the solid barrel 701 and the rotating circle 703 coincide, the rotating circle 703 is connected to the solid barrel 701 through a plurality of round rods, which is located directly below the turntable 2, a plurality of fixed teeth 704 are fixedly connected to the top of the rotating circle 703 in an annular manner with equal spacing, and the two ends of the fixed teeth 704 are triangular, and a motor 705 is fixedly installed on one side of the interior of the incubator 1, a disc 706 is sleeved on the output shaft of the motor 705, and a ratchet pawl mechanism 1 is provided between the output shaft of the motor 705 and the disc 706, as shown in FIG. Figure 4 As shown, the disc 706 can be driven to rotate only when the output shaft of the motor 705 rotates counterclockwise. The bottom of the disc 706 is rotatably connected to a rotating rod 707, which is eccentrically arranged on the disc 706. A fixing frame 708 is fixedly installed on the inner bottom of the incubator 1. A reciprocating block 709 is slidably connected in the fixing frame 708. A notch is arranged at the connection between the reciprocating block 709 and the fixing frame 708. One end of the rotating rod 707 away from the disc 706 is rotatably connected to the reciprocating block 709. Two paddles 710 are fixedly connected to the bottom of the reciprocating block 709. The two paddles 710 are staggered at the bottom of the reciprocating block 709. An inclined surface is arranged on one side of the two paddles 710 close to the fixed tooth 704. The two paddles 710 form a toggle fit with the fixed tooth 704 through the inclined surface.

[0045] Further, such as Figure 4 , Figure 7 As shown, a culture medium lifting assembly is provided at the bottom of the turntable 2, and the culture medium lifting assembly includes a pulley 801 sleeved on the output shaft of the motor 705, and a ratchet pawl mechanism 2 is provided at the connection between the pulley 801 and the output shaft of the motor 705, as shown in FIG. Figure 4As shown, only when the output shaft of the motor 705 rotates clockwise can the pulley 1 801 be driven to rotate. The incubator 1 is rotatably connected with the pulley 2 802, and the pulley 2 802 is located on the side away from the pulley 1 801. Belts are sleeved on the pulley 1 801 and the pulley 2 802. A reciprocating screw rod 803 is coaxially fixedly connected to the top of the pulley 2 802. A ratchet pawl assembly is provided at the connection between the pulley 2 802 and the reciprocating screw rod 803. A lifting seat 804 is threadedly connected to the reciprocating screw rod 803. A lever 805 is fixedly connected to the lifting seat 804 on the side close to the turntable 2. A toggle slot 806 is provided on the turntable 2, and the toggle slots 806 are arranged equidistantly. The toggle groove 806 is located directly below the culture medium seat 3 and the bottle seat 4. The number of the toggle grooves 806 is the same as the total number of the culture medium seat 3 and the bottle seat 4. The shape of the toggle groove 806 is the same as the toggle rod 805. The toggle rod 805 and the toggle groove 806 form a sliding fit. The side of the lifting seat 804 away from the toggle rod 805 is fixedly connected to the limiting block 807. A limiting groove 808 is provided in the incubator 1. The limiting groove 808 is located on the side of the incubator 1 close to the limiting block 807. The limiting block 807 is slidably connected to the limiting groove 808. The limiting block 807 cooperates with the limiting groove 808 so that the lifting seat 804 will not rotate under the action of the reciprocating screw rod 803, so that it can be lifted vertically.

[0046] Further, such as Fig. 9 , Figure 10 to Figure 12 As shown, a culture medium oscillation assembly is arranged below the turntable 2, and the culture medium oscillation assembly includes a mounting rod 901 fixedly connected to the incubator 1, and a wave plate 902 is fixedly connected to one end of the mounting rod 901 away from the incubator 1, and the wave plate 902 is located directly below the turntable 2, and two connecting rods 903 are fixedly connected to the bottom of the turntable 2, and the two connecting rods 903 are symmetrically arranged at the bottom of the turntable 2, and rollers 904 are arranged at the bottom of the two connecting rods 903, and a spring 905 is arranged in the solid tube 701, and the two ends of the spring 905 are respectively fixedly connected to the hexagonal rod 702 and the inner bottom of the incubator 1, and the wave plate 902 is ring-shaped, and the roller 904 forms a rolling fit with the wave plate 902.

[0047] In combination with the above embodiments, the entire working process and working principle of the above embodiments are as follows:

[0048] The initial state is:

[0049] A clean culture medium is installed in the culture medium seat 3, a liquid collection bottle 5 is installed in the bottle seat 4, and the spring 1 605 is extended, so that the slider 604 is located on the side of the fixed frame 603 close to the fixed tube 601, and the two baffles 606 are in contact, so that the bottom of the fixed tube 601 is closed, the push ring 610 is not in contact with the push block 608, the motor 705 is not started, and the lifting seat 804 is not raised, so that the lever 805 is located below the turntable 2, the roller 904 is located in the depression of the wave plate 902, and the spring 2 905 is not unfolded.

[0050] The working status is:

[0051] Turntable 2 quantitatively rotates to switch between culture medium and collection bottle 5:

[0052] When switching the container aligned with the bottom of 601, the motor 705 is started to make its output shaft rotate counterclockwise, so that the motor 705 drives the disk 706 on the output shaft to rotate under the action of the ratchet pawl mechanism 1, thereby making the disk 706 drive the rotating rod 707 to make eccentric movement. At this time, the reciprocating block 709 at the other end of the rotating rod 707 is pulled and slid, so the rotation of the disk 706 drives the reciprocating block 709 to slide back and forth in the fixed frame 708 through the rotating rod 707. When the reciprocating block 709 moves back and forth, the two misaligned paddles 710 successively push the fixed teeth 704 through the inclined surface, so that the fixed teeth 704 drive the rotating circle 703 to rotate under the cooperation of the inclined surface, thereby driving the turntable 2 to rotate intermittently and at a small distance during the continuous rotation of the disk 706, thereby achieving the effect of accurately aligning different containers with the bottom of the fixed tube 601, and then achieving the effect of automatic switching during the use of the device, which is convenient for use while ensuring the accuracy of the operation of inserting the strain.

[0053] To raise the medium to the bottom of the stationary tube:

[0054] When the filter membrane needs to be placed in the culture medium, the motor 705 is started to rotate its output shaft clockwise, thereby driving the pulley 1 801 to rotate under the action of the ratchet pawl mechanism 2, thereby driving the pulley 2 802 to rotate under the action of the belt, so that the pulley 2 802 drives the reciprocating screw 803 to rotate, thereby driving the lifting seat 804 to rise upward, and the lever 805 passes through the toggle groove 806 provided on the turntable 2 when rising upward, and then lifts the culture medium from the bottom of the culture medium in cooperation with the toggle groove 806, and drives the culture medium to rise upward under the drive of the reciprocating screw 803 until the culture medium contacts the push ring 610, at which time the motor 705 stops. The pulley 802 stops rotating, so that the lever 805 presses against the culture medium and fixes it at the bottom of the push ring 610. When the culture medium is at the bottom of the push ring 610, the height difference when the filter membrane falls from the fixed tube 601 is small, and the filter membrane will not be impacted when in contact with the culture medium due to the excessive height difference, thereby preventing the microorganisms on the filter membrane from splashing during the impact, and also preventing the filter membrane from wrinkling during the impact. Compared with the existing process for making bacterial culture medium that requires the use of more instruments, there is no need to use multiple sets of instruments, and the operation of manually transferring the filter membrane using tools is optimized, which simplifies the microbial filtration process while ensuring the standardization of the operation of placing the filter membrane into the culture medium.

[0055] Filter liquid and automatically drop the filter into the culture medium:

[0056] When it is necessary to add the microorganisms to be cultured into the culture medium, the filter membrane is placed in the fixed tube 601, and the motor 705 is used to switch the liquid collecting bottle 5 to the position directly below the fixed tube 601. At this time, the filter membrane falls onto the two baffles 606 under the action of gravity, and then the funnel used to introduce the liquid is screwed onto the threaded tube 602, and then the liquid containing the microorganisms is poured in, so that the liquid rinses the filter membrane and falls into the liquid collecting bottle 5 through the liquid outlet 607 on the baffle 606, so as to achieve the effect of collecting waste liquid. After the liquid is poured in, the microorganisms in the liquid are retained on the filter membrane under the filtering effect, and all the waste liquid enters the liquid collecting bottle 5 for collection. At this time, the motor 705 is used to drive the turntable 2 to rotate counterclockwise, so that the push ring 610 on the top of the culture medium first contacts the push block 608, and then the outer arc surface of the push ring 610 cooperates with the inclined surfaces of the two push blocks 608, so that the push block 6 08 pushes the baffle 606, so that the baffle 606 drives the slider 604 to slide in the fixed frame 603 to the side away from the fixed tube 601 and squeezes the spring 1 605. When the push ring 610 rotates to the bottom of the fixed tube 601, the two baffles 606 are fully opened. At this time, the filter membrane that loses its limit falls into the culture medium, thereby completing the operation of placing the filter membrane into the culture medium. In the interval between placing different culture media, the operator uses a flamethrower to sterilize the inside of the fixed tube 601, which can achieve the effect of guiding the user to perform the bacterial filtration operation in accordance with the specifications, simplifies the operation of the operator using tools to place the filter membrane on the limit meter for filtration, and does not require the operator to use tools to transfer the filter membrane from the limit meter to the culture medium and place it flat, thereby improving convenience and avoiding the occurrence of irregular operations, which is beneficial to the effect of microbial inoculant cultivation.

[0057] Remove the collected waste liquid:

[0058] During the process of placing multiple sets of filter membranes, waste liquids of different microorganisms are filtered out from the liquid outlet 607 and flow down, into different liquid collecting bottles 5 and stored. After the filter membranes are placed, the device can be opened and all the liquid collecting bottles 5 containing waste liquid can be taken out at one time, and then processed uniformly, which helps to simplify the cultivation process of microbial inoculants. Compared with traditional limit meters that require multiple one-way valves to be set inside, there is no need to set a one-way valve, and there is no need for the operator to manually rotate the switch, so as to achieve the effect of avoiding the backflow of waste liquid. Compared with the use of multiple sets of instruments, the use and manufacturing costs of the instruments are reduced, and the situation that the one-way valve in the limit meter is difficult to clean can be avoided.

[0059] To provide mixing of the medium after adding the nutrient solution:

[0060] During the cultivation of microbial inoculants, nutrient solution needs to be added regularly. The motor 705 is started and rotated counterclockwise to transfer the culture medium to which the nutrient solution needs to be added to the bottom of the fixed tube 601. At this time, the push ring 610 above the culture medium pushes the two push blocks 608 to open the two baffles 606. The motor 705 is started and rotated clockwise, so that the lever 805 raises the culture medium to the bottom of the fixed tube 601. At this time, the syringe filled with nutrient solution is inserted into the fixed tube 601 to add nutrient solution to the culture medium. After the nutrient solution is added to the culture medium, the culture medium and the lever 805 are reset. Then the motor 705 is started and rotated counterclockwise to drive the turntable 2 to rotate. The roller 904 at the bottom of the turntable 2 rolls along the wave plate 902. When the roller 904 rolls upward on the wave plate 902, the two rollers 904 prop up the turntable 2, so that the turntable 2 pulls the spring 2 905 through the hexagonal rod 702, so that the spring 2 905 stretches. When the roller 904 rolls downward on the wave plate 902, the turntable 2 moves downward under the action of its own gravity and the tightening of the spring 2 905, so that the turntable 2 continuously moves up and down during the rotation process, thereby achieving an oscillation effect, and then after the nutrient solution is added to the culture medium, the nutrient solution and the microorganisms are evenly mixed, thereby preventing the growth of microorganisms from being affected by the uneven flow of the nutrient solution.

[0061] The above descriptions are merely embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial inoculant culture instrument, characterized in that: include: An incubator (1), a turntable (2) and a culture medium seat (3), wherein the turntable (2) is arranged in the incubator (1), the culture medium seat (3) is fixedly connected to the top of the turntable (2), and a microbial filtration component is arranged above the turntable (2); The microorganism filtration assembly comprises a fixed tube (601) fixedly connected to the incubator (1), a threaded tube (602) fixedly connected to the top of the fixed tube (601), a fixed frame (603) fixedly connected to the threaded tube (602), at least two fixed frames (603) symmetrically arranged on the fixed tube (601), a slider (604) slidably connected inside the two fixed frames (603), and a A spring (605) is provided, the bottoms of the two sliders (604) are respectively fixedly connected with baffles (606), the two baffles (606) are provided with liquid outlet holes (607), the bottoms of the two baffles (606) are fixedly connected with push blocks (608), the top of the turntable (2) is fixedly connected with a fixing rod (609), the fixing rod (609) is symmetrically arranged on both sides of each culture medium seat (3), and the top of the fixing rod (609) is fixedly connected with a push ring (610).

2. A microbial inoculant culture instrument according to claim 1, characterized in that: The top of the turntable (2) is fixedly connected to a bottle seat (4), a liquid collecting bottle (5) is arranged inside the bottle seat (4), and the culture medium seat (3) and the bottle seat (4) are equidistantly arranged on the top of the turntable (2).

3. A microbial inoculant culture instrument according to claim 2, characterized in that: The push ring (610) has the same height as the push block (608), the two push blocks (608) are V-shaped, and the push ring (610) and the push blocks (608) form an extrusion fit.

4. The microbial inoculant culture instrument according to claim 1, characterized in that: A quantitative rotating assembly is provided at the bottom of the turntable (2), and the quantitative rotating assembly comprises a solid cylinder (701) rotatably connected to the bottom of the incubator (1), a hexagonal rod (702) is slidably connected inside the solid cylinder (701), the top of the hexagonal rod (702) is fixedly connected to the turntable (2), a rotating ring (703) is fixedly connected to the side of the solid cylinder (701), and a plurality of fixed teeth (704) are fixedly connected to the top of the rotating ring (703) at equal intervals in an annular manner, and one side of the interior of the incubator (1) is fixedly connected to the rotating ring (703). A motor (705) is fixedly installed, a disc (706) is sleeved on the output shaft of the motor (705), a rotating rod (707) is rotatably connected to the bottom of the disc (706), a fixed frame (708) is fixedly installed on the inner bottom of the incubator (1), a reciprocating block (709) is slidably connected inside the fixed frame (708), one end of the rotating rod (707) away from the disc (706) is rotatably connected to the reciprocating block (709), and two paddles (710) are fixedly connected to the bottom of the reciprocating block (709).

5. A microbial inoculant culture instrument according to claim 4, characterized in that: The fixed teeth (704) are arranged at equal distances.

6. A microbial inoculant culture instrument according to claim 5, characterized in that: The two paddles (710) are staggeredly arranged at the bottom of the reciprocating block (709); a slope is arranged on one side of the two paddles (710) close to the fixed tooth (704); and the two paddles (710) form a toggle fit with the fixed tooth (704) via the slope.

7. A microbial inoculant culture instrument according to claim 6, characterized in that: A culture medium lifting assembly is provided at the bottom of the turntable (2), and the culture medium lifting assembly includes a pulley 1 (801) sleeved on the output shaft of the motor (705), and a pulley 2 (802) is rotatably connected in the culture box (1), and the pulley 2 (802) is located on a side away from the pulley 1 (801), and a belt is sleeved on the pulley 1 (801) and the pulley 2 (802), and a reciprocating screw rod (803) is coaxially fixedly connected to the top of the pulley 2 (802), and the reciprocating screw rod (803) is provided on the reciprocating screw rod (803). A lifting seat (804) is threadedly connected, a side of the lifting seat (804) close to the turntable (2) is fixedly connected to a lever (805), a toggle slot (806) is provided on the turntable (2), a side of the lifting seat (804) away from the lever (805) is fixedly connected to a limiting block (807), a limiting slot (808) is provided in the incubator (1), the limiting slot (808) is located in the incubator (1) on a side close to the limiting block (807), and the limiting block (807) is slidably connected in the limiting slot (808).

8. A microbial inoculant culture instrument according to claim 7, characterized in that: The toggle grooves (806) are arranged at equal intervals, the number of the toggle grooves (806) is the same as that of the turntable (2) and the culture medium seat (3), the shape of the toggle grooves (806) is the same as that of the toggle rod (805), and the toggle rod (805) and the toggle grooves (806) form a sliding fit.

9. A microbial inoculant culture instrument according to claim 4, characterized in that: A culture medium oscillation assembly is arranged below the turntable (2), and the culture medium oscillation assembly includes a mounting rod (901) fixedly connected to the incubator (1), and the end of the mounting rod (901) away from the incubator (1) is fixedly connected to a wave plate (902), and two connecting rods (903) are fixedly connected to the bottom of the turntable (2), and the two connecting rods (903) are symmetrically arranged at the bottom of the turntable (2), and rollers (904) are arranged at the bottom of the two connecting rods (903), and a second spring (905) is arranged in the solid cylinder (701).

10. A microbial inoculant culture instrument according to claim 9, characterized in that: The wave plate (902) is ring-shaped, and the roller (904) forms a rolling fit with the wave plate (902).

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