Full-automatic intelligent cell culture device

Abstract

The invention discloses a full-automatic intelligent cell culture device which comprises a box body, a central gear rotatably inserted in the inner bottom wall of the box body and a plurality of driven gears, the driven gears are circumferentially distributed on the outer side of the central gear and are in meshing contact with the central gear, and the driven gears are coaxially and fixedly connected with a supporting disc; and a cell vessel is detachably arranged at the top of the supporting disc, the center gear is coaxially and fixedly connected with a driving rod, and the upper end of the driving rod is sleeved with an inner threaded sleeve in a threaded mode. According to the device, a cell culture solution and cells are placed in a cell vessel, then a motor is started to drive a central gear to rotate, and the central gear drives a driven gear to rotate, so that the cell vessel in a supporting disc rotates, the cells and the culture solution are uniformly shaken, and the motor drives a driving rod to synchronously rotate; the internal thread sleeve vertically moves in a reciprocating manner, so that the lower end of the sterile cotton swab is promoted to stir the cell dish in the vertical direction, and the uniform distribution effect is further improved.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a fully automatic intelligent cell culture device. Background Art

[0002] In the field of cell biology and life science research, cell culture is the most basic link. Cell culture is the process of growing and increasing the value of cells in an artificial environment using nutrients and other substances, and using them for "various purposes"; the fully automatic intelligent cell culture device is a device that uses advanced technology to culture cells, and is widely used in biomedicine, drug development, tissue engineering, regenerative medicine and other fields.

[0003] Cell culture is the basis of biological research and can be used for research in cell biology, molecular biology and biochemistry. During the cell culture process, for certain types of suspended cells (such as certain blood cells or tumor cells), it is crucial to maintain a uniform distribution of cells in the culture medium, and they usually need to be properly stirred to achieve the purpose of uniform distribution. However, the existing stirring method usually uses a stirring tool to contact the cell suspension, and the tool needs to be reused. The surface of the stirring tool may retain culture medium components, cell metabolites or other contaminants, which in turn affects cell growth and experimental results. Therefore, it is urgent to solve this problem. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a fully automatic intelligent cell culture device, which solves the problems raised in the above background technology.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a fully automatic intelligent cell culture device, comprising a box body, a central gear rotatably inserted in the bottom wall of the box body, and a plurality of driven gears, the driven gears are circumferentially distributed on the outside of the central gear and are in meshing contact with the central gear, the driven gear is coaxially fixedly connected with a support plate, a cell dish is detachably provided on the top of the support plate, the central gear is coaxially fixedly connected with a driving rod, an internally threaded sleeve is provided on the threaded sleeve at the upper end of the driving rod, a reciprocating thread is provided on the upper end of the driving rod, a bracket is fixedly connected to the upper end of the internally threaded sleeve, a plurality of clamping grooves are provided on the outer end of the bracket, a clamping assembly is provided inside the clamping groove, the upper end of a sterile cotton swab is provided in the clamping groove, and the sterile cotton swab is fixed by the clamping assembly.

[0008] Preferably, the clamping assembly includes a pull block and two pins fixed to the side walls of the pull block, the ends of the pins movably penetrate into the interior of the clamping groove, the two ends of the pins are fixedly connected with a pressure block, a spring is fixedly connected between the pull block and the end of the bracket, the spring is sleeved on the pins, and the upper end of the sterile cotton swab is pressed between the clamping groove and the pressure block.

[0009] Preferably, a motor is fixedly mounted on the bottom of the box, and an output end of the motor is coaxially fixedly connected to a central gear.

[0010] Preferably, a plurality of guide gas delivery components are fixedly installed between the top of the bracket and the top wall of the box body, and the guide gas delivery components include a guide sleeve fixed to the top of the bracket and a push rod movably inserted in the guide sleeve, and the upper end of the push rod is fixedly connected to the top wall of the box body.

[0011] Preferably, a one-way air inlet valve is connected to the side wall of the guide sleeve, an air pipe is connected to the guide sleeve, and a one-way air outlet valve is fixedly installed at the lower end of the air pipe.

[0012] Preferably, the numbers of the clamping grooves, sterile cotton swabs and cell dishes are the same.

[0013] (III) Beneficial effects

[0014] The present invention provides a fully automatic intelligent cell culture device, which has the following beneficial effects:

[0015] 1. In the present invention, the cell culture solution and cells are placed in the cell dish, and then the motor is started to drive the central gear to rotate, and the central gear drives the driven gear to rotate, so that the cell dish inside the support plate rotates, and the cells and the culture solution are evenly shaken. The motor drives the driving rod to rotate synchronously, so that the internal threaded sleeve moves back and forth vertically, prompting the lower end of the sterile cotton swab to move the cell dish in the vertical direction, further improving the effect of uniform distribution, and the sterile cotton swab is fixed by a clamping assembly, and the sterile cotton swab can be disassembled and replaced, and is disposable to avoid contamination of the culture solution and cells when used next time.

[0016] 2. In the present invention, when the rotating driving rod drives the internal threaded sleeve to move upward, the bracket moves upward synchronously, and the guide sleeve moves upward along the push rod, so that the gas inside the guide sleeve enters the trachea, and finally exits the trachea through the one-way air outlet valve and sprays into the cell dish, so as to perform bubble stirring on the cells and the culture medium inside the cell dish, avoid direct contact, and prevent contamination.

[0017] 3. In the present invention, the device can simultaneously realize the rotation of the cell dish, the vertical movement of the sterile cotton swab and the multiple stirring modes of the gas nozzle spraying gas, which can realize efficient and flexible cell culture operations; it combines different stirring modes together, which helps to improve the uniformity of cell growth and the success rate of the experiment, and is suitable for the needs of biological laboratories. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a front perspective structural diagram of a fully automatic intelligent cell culture device proposed by the present invention;

[0019] Figure 2 for Figure 1 A magnified structural diagram;

[0020] Figure 3 A top view of a cross-sectional structure of a fully automatic intelligent cell culture device proposed by the present invention;

[0021] Figure 4 A front cross-sectional structural diagram of a fully automatic intelligent cell culture device proposed by the present invention;

[0022] Figure 5 for Figure 4 The enlarged structure diagram at B;

[0023] Figure 6 for Figure 4 Enlarged structure diagram at C.

[0024] In the figure: 1. housing; 2. center gear; 3. driven gear; 4. motor; 5. support plate; 6. cell dish; 7. driving rod; 8. internal threaded sleeve; 9. clamping assembly; 901. pull block; 902. spring; 903. latch; 904. pressure block; 10. bracket; 1001. clamping groove; 11. sterile cotton swab; 12. guide air delivery assembly; 121. guide sleeve; 122. push rod; 13. air pipe; 14. one-way air inlet valve; 15. one-way air outlet valve. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0026] See also Figures 1 to 6The present invention provides a technical solution: a fully automatic intelligent cell culture device, comprising a box body 1, a central gear 2 rotatably inserted in the inner bottom wall of the box body 1, and a plurality of driven gears 3, the driven gears 3 are circumferentially distributed on the outer side of the central gear 2 and meshingly contact with the central gear 2, the driven gear 3 is coaxially fixedly connected with a support plate 5, the top of the support plate 5 is detachably provided with a cell dish 6, the top of the support plate 5 has a circular card slot, and the cell dish 6 is arranged inside the circular card slot; the central gear 2 is coaxially fixedly connected with a driving rod 7, the upper end of the driving rod 7 is provided with an internal threaded sleeve 8, the driving rod 7 A reciprocating thread is provided at the upper end, a bracket 10 is fixedly connected to the upper end of the internal threaded sleeve 8, a plurality of clamping grooves 1001 are provided at the outer end of the bracket 10, a clamping assembly 9 is provided inside the clamping groove 1001, the upper end of the sterile cotton swab 11 is arranged in the clamping groove 1001, and the sterile cotton swab 11 is fixed by the clamping assembly 9; the disposable sterile cotton swab 11 ensures that each use is under sterile conditions, which can effectively avoid cross contamination between different cell lines or samples; using a new cotton swab can prevent the introduction of bacteria, fungi or other microorganisms, and protect the purity of the cell culture medium.

[0027] Place the cell culture medium and cells into the cell dish 6, then start the motor 4 to drive the central gear 2 to rotate, and the central gear 2 drives the driven gear 3 to rotate, so that the cell dish 6 inside the support plate 5 rotates, and the cells and the culture medium are evenly shaken, and the motor 4 drives the driving rod 7 to rotate synchronously, so that the internal threaded sleeve 8 moves vertically back and forth, prompting the lower end of the sterile cotton swab 11 to move the cell dish 6 in the vertical direction, further improving the effect of uniform distribution, and the sterile cotton swab 11 is fixed by the clamping assembly 9, and the sterile cotton swab 11 can be disassembled and replaced, and is disposable to avoid contamination of the culture medium and cells when used next time.

[0028] Furthermore, the clamping assembly 9 includes a pull block 901, two latches 903 fixed to the side walls of the pull block 901, the ends of the latches 903 movably penetrate into the interior of the clamping groove 1001, the ends of the two latches 903 are fixedly connected with a pressing block 904, a spring 902 is fixedly connected between the pull block 901 and the end of the bracket 10, the spring 902 is sleeved on the latch 903, and the upper end of the sterile cotton swab 11 is pressed and arranged between the clamping groove 1001 and the pressing block 904.

[0029] Place the upper end of the sterile cotton swab 11 between the pressing block 904 and the clamping groove 1001, and then release the pull block 901. At this time, the spring 902 releases its elastic force, pulling the pull block 901 toward the clamping groove 1001, further making the pressing block 904 move toward the sterile cotton swab 11, and the pressing block 904 contacts the sterile cotton swab 11 to achieve the purpose of fixing the sterile cotton swab 11.

[0030] In order to drive the central gear 2 to rotate, a motor 4 is fixedly installed at the bottom of the box 1. The output end of the motor 4 is coaxially fixedly connected to the central gear 2. The motor 4 drives the central gear 2 to rotate, and the central gear 2 drives multiple driven gears 3 to rotate.

[0031] A plurality of guide gas delivery components 12 are fixedly installed between the top of the bracket 10 and the inner top wall of the box body 1. The guide gas delivery components 12 include a guide sleeve 121 fixed to the top of the bracket 10 and a push rod 122 movably inserted into the guide sleeve 121. The upper end of the push rod 122 is fixedly connected to the inner top wall of the box body 1.

[0032] A one-way air inlet valve 14 is connected to the side wall of the guide sleeve 121, and an air pipe 13 is connected to the guide sleeve 121. A one-way air outlet valve 15 is fixedly installed at the lower end of the air pipe 13; the one-way air inlet valve 14 only allows gas to enter the guide sleeve 121 to prevent the gas from flowing in the opposite direction; its setting ensures the one-way air flow of the system and avoids possible gas backflow, thereby improving the efficiency and safety of the system; the one-way air outlet valve 15 only allows gas to be discharged from the guide sleeve 121, ensuring the unidirectionality of the gas flow.

[0033] When the rotating driving rod 7 drives the internal threaded sleeve 8 to move upward, the bracket 10 moves upward synchronously, and the guide sleeve 121 moves upward along the push rod 122, so that the gas inside the guide sleeve 121 enters the trachea 13, and finally exits the trachea 13 through the one-way air outlet valve 15, and sprays into the cell dish 6, so as to perform gas stirring on the cells and the culture medium inside the cell dish 6 to avoid direct contact and prevent contamination. The shear force generated by gas stirring is usually small, which reduces the risk of mechanical damage to the cells, especially for those sensitive cells; when the guide sleeve 121 moves downward, the external air enters the guide sleeve 121 through the one-way air inlet valve 14, and the gas content inside the guide sleeve 121 increases.

[0034] In this solution, the number of the clamping grooves 1001, the sterile cotton swabs 11, and the cell dishes 6 is the same, specifically, six.

[0035] The device can simultaneously realize multiple stirring modes including the rotation of the cell dish 6, the vertical movement of the sterile cotton swab 11, and the gas ejection of the gas nozzle 13, and can realize efficient and flexible cell culture operations; it combines different stirring modes together, which helps to improve the uniformity of cell growth and the success rate of the experiment, and is suitable for the needs of biological laboratories.

[0036] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A fully automatic intelligent cell culture device, characterized in that: The invention comprises a housing (1), a central gear (2) rotatably inserted into the inner bottom wall of the housing (1), and a plurality of driven gears (3), wherein the driven gears (3) are circumferentially distributed outside the central gear (2) and mesh with the central gear (2), the driven gears (3) are coaxially fixedly connected to a support plate (5), a cell dish (6) is detachably provided on the top of the support plate (5), the central gear (2) is coaxially fixedly connected to a drive rod (7), and the drive rod (7) The upper threaded sleeve is provided with an internal threaded sleeve (8), the upper end of the driving rod (7) is provided with a reciprocating thread, the upper end of the internal threaded sleeve (8) is fixedly connected to a bracket (10), the outer end of the bracket (10) is provided with a plurality of clamping grooves (1001), a clamping assembly (9) is arranged inside the clamping groove (1001), the upper end of the sterile cotton swab (11) is arranged in the clamping groove (1001), and the sterile cotton swab (11) is fixed by the clamping assembly (9).

2. The fully automatic intelligent cell culture device according to claim 1, characterized in that: The clamping assembly (9) comprises a pulling block (901), two latches (903) fixed to the side walls of the pulling block (901), the ends of the latches (903) movably passing through the inside of the clamping groove (1001), the ends of the two latches (903) being fixedly connected with a pressing block (904), a spring (902) being fixedly connected between the pulling block (901) and the end of the bracket (10), the spring (902) being sleeved on the latches (903), and the upper end of the sterile cotton swab (11) being pressed and arranged between the clamping groove (1001) and the pressing block (904).

3. The fully automatic intelligent cell culture device according to claim 1, characterized in that: A motor (4) is fixedly mounted on the bottom of the box body (1), and an output end of the motor (4) is coaxially fixedly connected to the central gear (2).

4. The fully automatic intelligent cell culture device according to claim 1, characterized in that: A plurality of guide gas delivery components (12) are fixedly installed between the top of the bracket (10) and the inner top wall of the box body (1), and the guide gas delivery components (12) include a guide sleeve (121) fixed to the top of the bracket (10) and a push rod (122) movably inserted into the guide sleeve (121), and the upper end of the push rod (122) is fixedly connected to the inner top wall of the box body (1).

5. The fully automatic intelligent cell culture device according to claim 4, characterized in that: The side wall of the guide sleeve (121) is connected to a one-way air inlet valve (14), the guide sleeve (121) is connected to an air pipe (13), and the lower end of the air pipe (13) is fixedly mounted with a one-way air outlet valve (15).

6. The fully automatic intelligent cell culture device according to claim 2, characterized in that: The numbers of the clamping grooves (1001), sterile cotton swabs (11), and cell dishes (6) are the same.

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