A membrane transfer device for biochemical experiments

By designing a membrane transfer device for biochemical experiments, the coordinated movement of the drive assembly and the sliding assembly is used to solve the problem of uneven use of artificial scrapers, an efficient and uniform membrane transfer process is achieved, and the working efficiency of the laboratory is improved.

CN119164730BActive Publication Date: 2025-03-25YANGZHOU POLYTECHNIC COLLEGE
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Patent Information

Application Number
CN202411369331.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-29
Publication Date
2025-03-25
Estimated Expiration
2044-09-29

AI Technical Summary

Technical Problem

The prior art requires manual use of scrapers during membrane conversion, resulting in uneven force, wasting time and affecting laboratory work efficiency.

Method used

A membrane transfer device for biochemical experiments is designed, including mounting shell, drive assembly and sliding assembly. The sliding assembly and crossbar are driven to slide through the drive assembly, and the rubber storage plate and membrane transfer plate are simultaneously moved. The protein gel is uniformly scraped off by the rubber scraping assembly to reduce manual intervention.

Benefits of technology

The uniform movement of the rubber storage plate and the film transfer plate is achieved, and the balanced force of the rubber scraping assembly is shortened, the film transfer time is improved, the film transfer efficiency is saved, the laboratory operation time is improved, and the work efficiency is improved.

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Abstract

The invention discloses a membrane transfer device for biochemical experiments, which relates to the field of membrane transfer technology, including a mounting shell, a driving component and a sliding component fixedly connected to the mounting shell, two loading boxes and two unloading boxes slidably connected to the mounting shell, a plurality of glue storage plates slidingly arranged in one of the loading boxes, a plurality of membrane transfer plates slidingly arranged in the other loading box, a scraping component arranged on the mounting shell, the driving component drives the glue storage plates and the membrane transfer plates to transfer the membranes, and the membranes enter the unloading box for unloading after the membrane transfer, and the device can be reset after unloading, so that the device can be convenient for fast multiple membrane transfers, because the glue storage plates and the membrane transfer plates move evenly, when the scraping component is scraping the protein gel on the glue storage plates, the scraping force is balanced, so that the scraping time is saved, and the device can be quickly reset to perform multiple membrane transfers, so that the membrane transfer efficiency is increased, the time for multiple membrane transfers is saved, and the working efficiency of the laboratory is improved.
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Description

Technical Field

[0001] The invention relates to the technical field of membrane transfer, in particular to a membrane transfer device for biochemical experiments. Background Art

[0002] Western blotting (WB) is a commonly used protein detection method. It can accurately detect the presence and expression level of target proteins by separating and transferring proteins to a membrane and detecting them with specific antibodies. WB transfer is an important step in WB technology. It transfers the separated proteins to a solid support. Common supports include polyacrylamide gel, polyethylene glycol urea-formaldehyde membrane, etc. The purpose of WB transfer is to transfer proteins from the gel to the membrane for subsequent antibody detection.

[0003] After searching the publication number: CN214446765U, a WB transfer film cutting device with a platform includes a film-carrying platform for placing a WB transfer film, two film fixing devices are symmetrically arranged on the film-carrying platform, and the two film fixing devices can be moved on the film-carrying platform, and a film cutter is arranged on the film-carrying platform between the two film fixing devices, and the film cutter is arranged along the length direction of the film fixing device; the utility model has a simple structure and occupies a small space, and only needs to be assembled before use. Through the film-carrying platform and the film fixing device, it can be ensured that the film cutter is used to complete the shearing of the WB transfer film without touching the WB transfer film by human hands. The utility model avoids direct contact of human hands with the WB transfer film, increases the accuracy and efficiency of cutting, and improves the accuracy and efficiency of test results;

[0004] Currently, during the transfer process, it is usually necessary to manually use a scraper to scrape the glass plate where the protein gel is stored. However, it is difficult for humans to evenly control the force, and the protein gels on multiple glass plates often need to be transferred in the laboratory, which results in too much time being wasted on the transfer, affecting the laboratory's work efficiency. Summary of the invention

[0005] The purpose of the present invention is to provide a membrane transfer device for biochemical experiments to solve the deficiencies in the above-mentioned prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] In the above technical scheme, the present invention provides a transfer membrane device for biochemical experiments, including a mounting shell, a driving component and a sliding component are fixedly connected to the mounting shell, the driving component can drive the sliding component to slide, two loading boxes and two unloading boxes are slidably connected to the mounting shell, one of the loading boxes has a plurality of glue storage plates sliding inside, and the other loading box has a plurality of transfer membrane plates sliding inside, and the scraping component is arranged on the mounting shell,

[0008] When the driving component moves, it can drive the two cross bars in the sliding component to slide, and the two cross bars are slidably connected to the mounting shell. When the two cross bars slide, they drive one of the glue storage plates and the transfer plate to move synchronously. When one of the glue storage plates and the transfer plate move synchronously, the scraper component transfers the protein gel on the glue storage plate to the transfer plate. When one of the glue storage plates and the transfer plate move one by one to the two discharge boxes respectively, a clamping unit is provided on each of the two discharge boxes, so that the two cross bars cannot drive the glue storage plate and the transfer plate to move when they are reset, and at the same time, an arc surface is provided on the glue storage plate so that it will not affect the entry of the next glue storage plate.

[0009] Furthermore, the driving assembly includes a driving source, which is arranged inside the mounting shell. A threaded rod is arranged on the output end of the driving source. The end of the threaded rod away from the driving source is rotatably connected to the mounting shell. A threaded sleeve is threadedly connected to the threaded rod, and the threaded sleeve is connected to the two cross bars.

[0010] Furthermore, a plurality of first telescopic members are arranged in the cross bar, and output ends of the plurality of first telescopic members are fixedly connected to a clamping rod, and a side of the clamping rod away from the first telescopic member penetrates to the outside of the cross bar.

[0011] Furthermore, the two loading box openings are arranged corresponding to each other, and the loading box includes a first box body, a first sliding member is arranged on the first box body, the first sliding member is slidably connected to the mounting shell, a first support plate is arranged at the box opening of the first box body, a first support frame is arranged on the side of the first support plate away from the first box body, and the first support frame is arranged as an opening on a side facing the scraper assembly, and the first support frames on the two loading boxes are fitted with each other.

[0012] Furthermore, a first slot is provided near the opening of the feeding box, a baffle is rotatably connected to the first slot, a chamber is provided in the wall of the box body, a rotating shaft is provided on the baffle, the rotating shaft passes through the chamber, a first gear is provided at one end of the rotating shaft located in the chamber, a second telescopic member is provided in the chamber, a connecting plate is provided at the output end of the second telescopic member, a rack is provided on a side of the connecting plate away from the second telescopic member, the rack is meshed with the first gear, a side of the rack away from the connecting plate passes through the outside of the box body, and an arc block is provided on a side of the rack away from the connecting plate.

[0013] Furthermore, the adhesive storage plate includes a glass plate, a first limit block is provided on both sides of the glass plate, one of the first limit blocks is provided with a clamping groove, and the transfer plate includes a support plate, a second limit block is provided on both sides of the support plate, one of the second limit blocks is provided with a clamping groove, and the two clamping rods can enter the two clamping grooves one by one when moving.

[0014] Furthermore, the driving assembly also includes a second gear sleeved on the output end of the driving source, a third gear is arranged in the mounting shell, the third gear is meshed with the second gear, a transmission shaft is fixedly connected to the third gear, and the transmission shaft is rotatably connected to the inside of the mounting shell, a first bevel gear is arranged at an end of the transmission shaft away from the third gear, the first bevel gear, a tooth chain is arranged in the mounting shell, a plurality of fourth gears are meshed in the tooth chain, a second bevel gear is arranged on one of the fourth gears, the first bevel gear is meshed with the second bevel gear, a plurality of fourth gears are respectively connected with a plurality of connecting shafts in a one-to-one correspondence, and a plurality of connecting shafts at one end away from the fourth gear all penetrate and are rotatably connected to the outside of the mounting shell, a supporting member is arranged on the mounting shell, a plurality of rollers are rotatably connected to the supporting member, and a plurality of rollers are respectively connected with a plurality of connecting shafts in a one-to-one correspondence, and a scraper is arranged on the supporting member.

[0015] Furthermore, the two openings of the lower material boxes are facing each other, and the two openings of the lower material boxes are arranged corresponding to each other. The upper material box includes a second box body, and a second sliding member is arranged on the second box body. The second sliding member is slidably connected to the mounting shell, and a second support plate is arranged at the box opening of the second box body. A second support frame is arranged on the side of the second support plate away from the second box body, and the side of the second support frame facing the scraper assembly is arranged as an opening, and the second support frames on the two lower material boxes fit together.

[0016] Furthermore, the clamping unit includes a third telescopic member arranged on the second box body, a fixing plate is arranged at an output end of the third telescopic member, and an inclined block is arranged at one end of the fixing plate away from the third telescopic member.

[0017] Furthermore, it also includes a shock absorbing unit, a fourth telescopic member is arranged in the second box body, a top plate is arranged on the top of the fourth telescopic member, and a sponge pad is arranged on the top of the top plate.

[0018] In the above technical scheme, the present invention provides a transfer device for biochemical experiments, which drives the sliding component to slide through the driving component, drives the gelatin storage plate and the transfer plate to transfer the film through the movement of the sliding component, and enters the material box for unloading after the film transfer. At the same time, the device can be reset after unloading, which is convenient for the device to quickly transfer the film multiple times. Since the gelatin storage plate and the transfer plate move evenly, the scraping force of the scraping component is balanced when scraping the protein gel on the gelatin storage plate, so that the scraping time is saved, and the device can be quickly reset to transfer the film multiple times, so that the transfer efficiency is increased, saving the time for multiple transfers, thereby improving the work efficiency of the laboratory. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0020] Figure 1 One of the main structural diagrams provided by an embodiment of the present invention;

[0021] Figure 2 The second schematic diagram of the main structure provided by the embodiment of the present invention;

[0022] Figure 3 A schematic diagram of the main structure of the material box provided in an embodiment of the present invention;

[0023] Figure 4 A schematic diagram of a loading box body provided in an embodiment of the present invention;

[0024] Figure 5 The embodiment of the present invention provides Figure 4 A schematic diagram of the enlarged structure at point A;

[0025] Figure 6 A schematic diagram of the structure of the glue storage plate and the film transfer plate provided in an embodiment of the present invention;

[0026] Figure 7 A schematic diagram of the cross-sectional structure of a material box provided in an embodiment of the present invention;

[0027] Figure 8 A schematic diagram of the structure of a loading box provided in an embodiment of the present invention;

[0028] Fig. 9 A schematic diagram of the structure of a sliding assembly provided in an embodiment of the present invention;

[0029] Fig.10 A schematic diagram of a cross-sectional structure of a sliding assembly provided in an embodiment of the present invention;

[0030] Fig.11 One of the internal structure schematic diagrams provided by the embodiment of the present invention;

[0031] Fig.12 The second internal structure diagram provided for the embodiment of the present invention.

[0032] Description of reference numerals:

[0033] 1. Mounting shell; 2. Driving assembly; 21. Driving source; 22. Threaded rod; 23. Threaded sleeve; 24. Second gear; 25. Third gear; 26. Transmission shaft; 27. First bevel gear; 28. Toothed chain; 29. ​​Fourth gear; 210. Second bevel gear; 211. Connecting shaft; 3. Sliding assembly; 31. Crossbar; 32. First telescopic member; 33. Clamping rod; 4. Loading box; 41. First box body; 42. First sliding member; 43. First support plate; 44. First support frame; 45. Baffle; 46. Rotating shaft; 47. First gear; 48. Second telescopic member; 49. Connecting plate; 410. Rack; 411. Arc block; 5. Feed box; 51. Second box body; 52. Second sliding member; 53. Second supporting plate; 54. Second supporting frame; 55. Clamping unit; 551. Third telescopic member; 552. Fixed plate; 553. Oblique block; 56. Shock-absorbing unit; 561. Fourth telescopic member; 562. Top plate; 563. Sponge pad; 6. Glue storage plate; 61. Glass plate; 62. First limiting block; 7. Transfer plate; 71. Support plate; 72. Second limiting block; 8. Glue scraping assembly; 81. Support member; 82. Roller; 83. Scraper. DETAILED DESCRIPTION

[0034] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0035] See also Figure 1-12 The embodiment of the present invention provides a transfer membrane device for biochemical experiments, including a mounting shell 1, a driving component 2 and a sliding component 3 are fixedly connected to the mounting shell 1, the driving component 2 can drive the sliding component 3 to slide, and two loading boxes 4 and two unloading boxes 5 are slidably connected to the mounting shell 1, a plurality of glue storage plates 6 are slidably arranged in one of the loading boxes 4, and a plurality of transfer membrane plates 7 are slidably arranged in the other loading box 4,

[0036] When the driving component 2 moves, it can drive the two cross bars 31 in the sliding component 3 to slide. The two cross bars 31 are both slidably connected to the mounting shell 1. When the two cross bars 31 slide, they drive a glue storage plate 6 and a transfer plate 7 to move synchronously. When a glue storage plate 6 and a transfer plate 7 move synchronously, the scraper component 8 transfers the protein gel on the glue storage plate 6 to the transfer plate 7. When a glue storage plate 6 and a transfer plate 7 move one by one to two unloading boxes 5 respectively, a clamping unit 55 is set on each of the two unloading boxes 5, so that the two cross bars 31 cannot drive the glue storage plate 6 and the transfer plate 7 to move when resetting, and at the same time, a curved surface is set on the glue storage plate 6 so that it will not affect the entry of the next glue storage plate 6.

[0037] Preferably, the driving assembly 2 includes a driving source 21, which is arranged inside the mounting shell 1. A threaded rod 22 is arranged on the output end of the driving source 21. The end of the threaded rod 22 away from the driving source 21 is rotatably connected to the mounting shell 1. A threaded sleeve 23 is threadedly connected to the threaded rod 22. The threaded sleeve 23 is connected to two cross bars 31. The sliding connection between the cross bar 31 and the mounting shell 1 enables the threaded sleeve 23 to slide. At the same time, the two cross bars 31 are driven to slide synchronously under the action of the threaded sleeve 23, so that the glue storage plate 6 and the film transfer plate 7 can move synchronously, thereby preventing the film transfer failure caused by the misalignment between the glue storage plate 6 and the film transfer plate 7.

[0038] Preferably, a plurality of first telescopic members 32 are provided in the cross bar 31, and the output ends of the plurality of first telescopic members 32 are fixedly connected to a clamping rod 33. The clamping rod 33 extends to the outside of the cross bar 31 from the side away from the first telescopic members 32. An arc surface is provided on one side of the clamping rod 33, so that the clamping rod 33 will not get stuck due to contact with the glue storage plate 6 or the film transfer plate 7 when resetting, and the clamping rod 33 will be retracted into the cross bar 31.

[0039] Preferably, the box openings of the two feeding boxes 4 are arranged corresponding to each other, the feeding box 4 includes a first box body 41, and the first sliding member 42 is arranged on the first box body 41, and the first sliding member 42 is slidably connected to the mounting shell 1, and a first supporting plate 43 is arranged at the box opening of the first box body 41, and a first supporting frame 44 is arranged on the side of the first supporting plate 43 away from the first box body 41, and the first supporting frame 44 is arranged to be open on the side facing the scraper assembly 8, and the first supporting frames 44 on the two feeding boxes 4 fit each other, and the positional relationship between the glue storage plate 6 and the film transfer plate 7 is better controlled by the arrangement of the first supporting frames 44, so as to prevent the glue storage plate 6 and the film transfer plate 7 from being unable to be clamped with the clamping rod 33 due to position problems, and By setting the first support plate 43, there is a gap between the first support frame 44 and the box opening, so that the clamping grooves on the glue storage plate 6 and the transfer plate 7 can leak out, which is convenient for them to be clamped with the clamping rod 33. At the same time, a box cover is set at a position where the two loading boxes 4 are far away from each other, and the box cover and the first box body 41 can be clamped to close or open, and the two loading boxes 4 are slidably connected with the mounting shell 1, so that the loading boxes 4 are convenient to disassemble and load. At the same time, a spring is set on the inner box cover of one of the loading boxes 4 located at the bottom, and a top plate 562 is set on the spring, so that the transfer plate 7 can always be in contact with the first frame to ensure that its position will not change due to the number of transfer plates 7 in the loading box 4.

[0040] Preferably, the loading box 4 is provided with a first notch near the opening, a baffle 45 is rotatably connected to the first notch, a chamber is provided in the wall of the box body, a rotating shaft 46 is provided on the baffle 45, the rotating shaft 46 penetrates into the chamber, a first gear 47 is provided at one end of the rotating shaft 46 located in the chamber, a second telescopic member 48 is provided in the chamber, a connecting plate 49 is provided at the output end of the second telescopic member 48, a rack 410 is provided on the side of the connecting plate 49 away from the second telescopic member 48, the rack 410 is meshed with the first gear 47, the side of the rack 410 away from the connecting plate 49 penetrates to the outside of the box body, and the rack 410 An arc block 411 is provided on the side away from the connecting plate 49. By setting the two sides of the arc block to be inclined surfaces, the arc block will not affect the movement of the cross bar 31 when it is reset. When in use, a torsion spring is provided at the rotation connection between the baffle 45 and the first box body 41. The setting of the baffle 45 limits the positions of the glue storage plate 6 and the transfer plate 7 when the film transfer is not performed. After the two clamping rods 33 are clamped with the glue storage plate 6 and the transfer plate 7, the arc block is squeezed by the cross bar 31, so that the baffle 45 is opened, so that the baffle 45 does not affect the movement of the glue storage plate 6 and the transfer plate 7 when the film is transferred.

[0041] Preferably, the glue storage plate 6 includes a glass plate 61, and a first limit block 62 is provided on both sides of the glass plate 61, and a clamping groove is provided on one of the first limit blocks 62, and the transfer plate 7 includes a support plate 71, and a second limit block 72 is provided on both sides of the support plate 71, and a clamping groove is provided on one of the second limit blocks 72, and the two clamping rods 33 can enter the two clamping grooves one by one when moving, and at the same time, a plastic arc block is provided on one side of the glass plate 61, so that when the next glue storage plate 6 is about to enter the discharge box 5, it will contact the inclined surface of the previous glue storage plate 6, so that the previous glue storage plate 6 is lifted, so that the travel path of the next glue storage plate 6 will not be affected, and the transfer plate 7 falls under the action of gravity, and the shock absorption of the fourth telescopic member 561 and the sponge pad 563 is used to successfully collect the transfer plate 7 after the transfer is completed.

[0042] Preferably, the driving assembly 2 also includes a second gear 24 sleeved on the output end of the driving source 21, a third gear 25 is provided in the mounting shell 1, the third gear 25 is meshed with the second gear 24, a transmission shaft 26 is fixedly connected to the third gear 25, the transmission shaft 26 is rotatably connected to the inside of the mounting shell 1, and a first bevel gear 27 is provided at one end of the transmission shaft 26 away from the third gear 25. The first bevel gear 27 and a toothed chain 28 are provided in the mounting shell 1, and a plurality of fourth gears 29 are meshed in the toothed chain 28, a second bevel gear 210 is provided on one of the fourth gears 29, the first bevel gear 27 is meshed with the second bevel gear 210, and a plurality of connecting shafts 211 are respectively connected to the plurality of fourth gears 29 in a one-to-one correspondence, and the ends of the plurality of connecting shafts 211 away from the fourth gear 29 all penetrate and are rotatably connected to the outside of the mounting shell 1. A support member 81 is provided on the mounting shell 1, and a plurality of rollers 82 are rotatably connected to the support member 81. The plurality of rollers 82 are respectively connected to a plurality of connecting shafts 211 in a one-to-one correspondence. A scraper 83 is provided on the support member 81, and the scraper 83 is close to the surface of the gel storage plate 6 on which the protein gel is placed to scrape the protein gel. After the protein gel is scraped off, it falls on the membrane prepared on the transfer plate 7, and at the same time, the scraped portion of the protein gel contacts with the roller 82, and when the driving source 21 rotates, it drives the second gear 24 to rotate, so that the third gear 25 rotates, and drives the plurality of rollers 82 to start rotating at the same time. By setting a speed change relationship between the second gear 24 and the third gear 25, the rolling rotation will not tear the protein gel, and the bubbles between the protein gel and the membrane are fully discharged by squeezing the protein gel by the roller 82.

[0043] Preferably, the box openings of the two unloading boxes 5 face each other, and the box openings of the two unloading boxes 5 are arranged corresponding to each other. The loading box 4 includes a second box body 51, and a second sliding member 52 is arranged on the second box body 51. The second sliding member 52 is slidably connected to the mounting shell 1. A second support plate 53 is arranged at the box opening of the second box body 51, and a second support frame 54 is arranged on the side of the second support plate 53 away from the second box body 51. The second support frame 54 is arranged to have an opening on the side facing the scraper assembly 8. The second support frames 54 on the two unloading boxes 5 fit each other, and box covers are also arranged at the places where the two second box bodies 51 are away from each other, so as to facilitate the removal of the glue storage plate 6 and the film transfer plate 7 after the film transfer is completed.

[0044] Preferably, the clamping unit 55 includes a third telescopic member 551 arranged on the second box body 51, and a fixed plate 552 is arranged at the output end of the third telescopic member 551, and an inclined block 553 is arranged at the end of the fixed plate 552 away from the third telescopic member 551. After the film transfer, the two inclined blocks 553 of the glue storage plate 6 and the film transfer plate 7 contact each other, so that the two inclined blocks 553 move, driving the third telescopic member 551 to contract, and then the glue storage plate 6 and the film transfer plate 7 continue to move until they enter the two unloading boxes 5 one by one respectively. When the glue storage plate 6 and the film transfer plate 7 completely enter the two unloading boxes 5, the two third telescopic members 551 are released, so that the two inclined blocks 553 move downward to limit the glue storage plate 6 and the film transfer plate 7, so that the glue storage plate 6 and the film transfer plate 7 are both limited at this time, so that the subsequent resetting of the sliding assembly 3 will not drive the movement of the glue storage plate 6 and the film transfer plate 7, so that the device can be reset for the next film transfer.

[0045] Preferably, it also includes a shock absorbing unit 56, a fourth telescopic member 561 is arranged in the second box body 51, a top plate 562 is arranged on the top of the fourth telescopic member 561, a sponge pad 563 is arranged on the top of the top plate 562, and the fourth telescopic member 561 is arranged on the box cover in the second box body 51, so that the shock absorbing unit 56 can be brought out when the box cover is opened, thereby preventing the shock absorbing unit 56 from affecting the removal of the transfer plate 7.

[0046] Working principle:

[0047] The driving source 21 is started to drive the threaded rod 22 to rotate, so that the threaded sleeve 23 slides, and the two cross bars 31 slide, so that the first telescopic members 32 slide, and the two clamping rods 33 slide. When sliding, the two clamping rods 33 enter the two clamping grooves one by one, so that a glue storage plate 6 and a film transfer plate 7 move synchronously. When moving, the two cross bars 31 contact the two arc blocks 411 respectively, so that the two arc blocks 411 shrink into the chamber, and drive the two racks 410 to move, so that the two connecting plates 49 move, and drive the two second telescopic members 48 to shrink. When the two racks 410 move, they drive the two first gears 47 to rotate, so that the two rotating shafts 46 rotate, and drive the two baffles 45 to rotate and open. , so that the baffle 45 no longer limits the gel storage plate 6 or the transfer plate 7, and then the gel storage plate 6 contacts the scraper 83 when it moves, and the scraper 83 is close to the surface of the gel storage plate 6 where the protein gel is placed to scrape the protein gel, and the protein gel falls on the prepared film on the transfer plate 7 after being scraped off, and at the same time, the scraped part of the protein gel contacts the roller 82, and when the driving source 21 rotates, it drives the second gear 24 to rotate, so that the third gear 25 rotates, drives the rotating shaft to rotate, so that the first bevel gear 27 rotates, drives the second bevel gear 210 to rotate, and drives a fourth gear 29 to rotate, drives the chain to rotate, so that a plurality of fourth bevel gears rotate synchronously, drives a plurality of connecting shafts 211 to rotate, and causes a plurality of rollers 82 to start rotating at the same time, and by setting the second gear 2 4 and the third gear 25, so that the rolling rotation will not tear the protein gel, and the bubbles between the protein gel and the membrane are fully discharged by squeezing the protein gel by the roller 82. After the film is transferred, the glue storage plate 6 and the film transfer plate 7 continue to move and contact the two inclined blocks 553 one by one, respectively, so that the two inclined blocks 553 move, driving the third telescopic member 551 to contract, and then the glue storage plate 6 and the film transfer plate 7 continue to move until they enter the two unloading boxes 5 one by one respectively. When the glue storage plate 6 and the film transfer plate 7 completely enter the two unloading boxes 5, the two third telescopic members 551 are released, so that the two inclined blocks 553 move downward to limit the glue storage plate 6 and the film transfer plate 7, and then the driving source 21 reverses to drive the two cross bars 31 to slide, so that the two clamping rods 33 move in The two sides of the arc block are arranged in an arc shape, so that the arc block does not affect the movement of the cross bar 31. When the clamping rod 33 moves to no longer contact the glue storage plate 6 and the film transfer plate 7, the film transfer plate 7 falls under the action of gravity. The fourth telescopic member 561 and the sponge pad 563 are used for shock absorption, so that the film transfer plate 7 is successfully collected after the film transfer is completed. At the same time, a plastic inclined surface is set on one side of the glue storage plate 6, so that when the next glue storage plate 6 is about to enter the unloading box 5, it will contact the inclined surface of the previous glue storage plate 6, so that the previous glue storage plate 6 is lifted up, so that the travel path of the next glue storage plate 6 will not be affected. When the two clamping rods 33 move to contact the glue storage plates 6 and the film transfer plates 7 in the two loading boxes 4 respectively, by setting one side of the clamping rod 33 as an arc surface,The clamping rod 33 is compressed and retracted into the cross bar 31, so that the first telescopic parts 32 are compressed until the two clamping rods 33 are no longer in contact with the storage plastic plate 6 and the film transfer plate 7 in the two loading boxes 4. Then, the first telescopic parts 32 are released, so that the two clamping rods 33 are reset, so that the whole device is reset and the next film transfer can be carried out quickly.

[0048] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A membrane transfer device for biochemical experiments, comprising a mounting shell (1), characterized in that: The mounting shell (1) is fixedly connected with a driving component (2) and a sliding component (3), and the driving component (2) can drive the sliding component (3) to slide. The mounting shell (1) is slidably connected with two loading boxes (4) and two unloading boxes (5), one of the loading boxes (4) has a plurality of glue storage plates (6) sliding inside, and the other loading box (4) has a plurality of film transfer plates (7) sliding inside. The scraping component (8) is arranged on the mounting shell (1). When the driving component (2) moves, it can drive the two cross bars (31) in the sliding component (3) to slide. The two cross bars (31) are both slidably connected to the mounting shell (1). When the two cross bars (31) slide, they drive one of the glue storage plates (6) and one of the transfer plates (7) to move synchronously. When one of the glue storage plates (6) and one of the transfer plates (7) move synchronously, the scraper component (8) transfers the protein gel on the glue storage plate (6) to the transfer plate (7). When one of the glue storage plates (6) and one of the transfer plates (7) move one by one into the two material discharge boxes (5), a clamping unit (55) is provided on each of the two material discharge boxes (5), so that the two cross bars (31) cannot drive the glue storage plate (6) and the transfer plate (7) to move when they are reset. At the same time, an arc surface is provided on the glue storage plate (6) so that it will not affect the entry of the next glue storage plate (6).

2. A biochemical experiment transfer device according to claim 1, characterized in that: The driving assembly (2) comprises a driving source (21), the driving source (21) being arranged inside the mounting shell (1), a threaded rod (22) being arranged on the output end of the driving source (21), one end of the threaded rod (22) being away from the driving source (21) being rotatably connected to the mounting shell (1), a threaded sleeve (23) being threadedly connected to the threaded rod (22), and the threaded sleeve (23) being connected to the two cross bars (31).

3. A biochemical experiment transfer device according to claim 2, characterized in that: A plurality of first telescopic members (32) are arranged inside the cross bar (31), and output ends of the plurality of first telescopic members (32) are fixedly connected to a clamping rod (33), and a side of the clamping rod (33) away from the first telescopic member (32) penetrates to the outside of the cross bar (31).

4. A biochemical experiment transfer device according to claim 1, characterized in that: The box openings of the two loading boxes (4) are arranged corresponding to each other, and the loading box (4) includes a first box body (41), and a first sliding member (42) is arranged on the first box body (41), and the first sliding member (42) is slidably connected to the mounting shell (1), and a first support plate (43) is arranged at the box opening of the first box body (41), and a first support frame (44) is arranged on the side of the first support plate (43) away from the first box body (41), and the first support frame (44) is arranged to be open on the side facing the scraper assembly (8), and the first support frames (44) on the two loading boxes (4) are in contact with each other.

5. A biochemical experiment transfer device according to claim 4, characterized in that: A first slot is provided near the opening of the loading box (4), and a baffle (45) is rotatably connected to the first slot. A chamber is provided in the wall of the box body, and a rotating shaft (46) is provided on the baffle (45). The rotating shaft (46) passes through the chamber. A first gear (47) is provided at one end of the rotating shaft (46) located in the chamber. A second telescopic member (48) is provided in the chamber, and a connecting plate (49) is provided at the output end of the second telescopic member (48). A rack (410) is provided on the side of the connecting plate (49) away from the second telescopic member (48), and the rack (410) is meshed with the first gear (47). The side of the rack (410) away from the connecting plate (49) passes through the outside of the box body, and an arc block (411) is provided on the side of the rack (410) away from the connecting plate (49).

6. A biochemical experiment transfer device according to claim 3, characterized in that: The film storage plate (6) includes a glass plate (61), and a first limit block (62) is provided on both sides of the glass plate (61), and one of the first limit blocks (62) is provided with a clamping groove. The film transfer plate (7) includes a support plate (71), and a second limit block (72) is provided on both sides of the support plate (71), and one of the second limit blocks (72) is provided with a clamping groove. The two clamping rods (33) can respectively enter the two clamping grooves one by one when moving.

7. A biochemical experiment transfer device according to claim 2, characterized in that: The driving assembly (2) further comprises a second gear (24) sleeved on the output end of the driving source (21); a third gear (25) is arranged in the mounting shell (1); the third gear (25) meshes with the second gear (24); a transmission shaft (26) is fixedly connected to the third gear (25); the transmission shaft (26) is rotatably connected to the inside of the mounting shell (1); a first bevel gear (27) is arranged at one end of the transmission shaft (26) away from the third gear (25); the first bevel gear (27); a toothed chain (28) is arranged in the mounting shell (1); a plurality of fourth gears (29) mesh with the toothed chain (28); one of the fourth gears (29) is meshed with the toothed chain (28); A second bevel gear (210) is arranged on the fourth gear (29), the first bevel gear (27) is meshed with the second bevel gear (210), a plurality of the fourth gears (29) are respectively connected to a plurality of connecting shafts (211) in a one-to-one correspondence, and the ends of the plurality of connecting shafts (211) away from the fourth gear (29) are all penetrated and rotatably connected to the outside of the mounting shell (1), a support member (81) is arranged on the mounting shell (1), a plurality of rollers (82) are rotatably connected to the support member (81), and the plurality of rollers (82) are respectively connected to the plurality of connecting shafts (211) in a one-to-one correspondence, and a scraper (83) is arranged on the support member (81).

8. The membrane transfer device for biochemical experiments according to claim 1, characterized in that: The box openings of the two material lowering boxes (5) are facing each other, and the box openings of the two material lowering boxes (5) are arranged corresponding to each other. The material upper box (4) includes a second box body (51), and a second sliding member (52) is arranged on the second box body (51). The second sliding member (52) is slidably connected to the mounting shell (1). A second support plate (53) is arranged at the box opening of the second box body (51), and a second support frame (54) is arranged on the side of the second support plate (53) away from the second box body (51). The side of the second support frame (54) facing the scraper assembly (8) is arranged to be open, and the second support frames (54) on the two material lowering boxes (5) are in contact with each other.

9. A biochemical experiment transfer device according to claim 8, characterized in that: The clamping unit (55) comprises a third telescopic member (551) arranged on the second box body (51); a fixing plate (552) is arranged at the output end of the third telescopic member (551); and an inclined block (553) is arranged at one end of the fixing plate (552) away from the third telescopic member (551).

10. A biochemical experiment transfer device according to claim 9, characterized in that: It also includes a shock absorbing unit (56), a fourth telescopic member (561) is arranged in the second box body (51), a top plate (562) is arranged on the top of the fourth telescopic member (561), and a sponge pad (563) is arranged on the top of the top plate (562).

Citation Information

Patent Citations

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