Liquid chromatography tandem mass spectrometry detection system and waste gas emission method

Abstract

The embodiment of the present application provides a liquid chromatography-tandem mass spectrometry detection system and an exhaust gas emission method, the system including a mass spectrometer, a liquid chromatograph and a controller. The mass spectrometer includes an ion source and an exhaust gas emission device, the ion source is connected to the liquid chromatograph; the exhaust gas emission device includes a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel and a turbine component; the exhaust gas emitted by the ion source enters the inlet of the first exhaust gas channel; the inlet of the heat dissipation component is connected to the outlet of the first exhaust gas channel for cooling the exhaust gas; the inlet of the second exhaust gas channel is connected to the outlet of the heat dissipation component; the turbine component is arranged at the outlet of the second exhaust gas channel to control the cooled exhaust gas to be discharged through the outlet of the second exhaust gas channel. The controller obtains the liquid phase parameters of the chromatograph and the ion source parameters of the ion source to confirm the exhaust gas emission rate of the mass spectrometer and the target speed of the turbine component. The turbine component is controlled to match different speeds under different detection objects, which greatly improves the sensitivity of the mass spectrometer.

Description

Technical Field

[0001] The present invention relates to the technical field of mass spectrometry equipment, and in particular to a liquid chromatography tandem mass spectrometry detection system and an exhaust gas emission method. Background Art

[0002] Mass spectrometers are instruments used to separate and detect different inorganic and organic compounds. Liquid chromatography tandem mass spectrometry (LC-MS / MS) detection systems combine the high separation capabilities of liquid chromatography with the highly sensitive and selective detection capabilities of a tandem mass spectrometer. They are widely used in fields such as life sciences, pharmaceutical research and development, food safety, and environmental monitoring.

[0003] During the use of liquid chromatography-tandem mass spectrometry (LC-MS / MS), the ion source generates a large amount of waste solvent and sample during the ionization process. These substances reflux within the sealed ion source chamber, causing memory effects and contamination of the ion source, thereby affecting the sensitivity of the instrument. Therefore, there is an urgent need for a device and method for flexibly discharging waste gas from the mass spectrometer in a liquid chromatography-tandem mass spectrometry detection system. Summary of the Invention

[0004] The present application discloses a liquid chromatography-tandem mass spectrometry detection system and an exhaust gas emission method, which are intended to solve the problem that a large amount of waste solvents and samples are generated during the ionization process of the current ion source. These substances reflux in the closed ion source cavity, causing memory effects and contamination of the ion source, thereby affecting the sensitivity of the instrument.

[0005] First invention, the present application provides a liquid chromatography tandem mass spectrometry detection system, the liquid chromatography tandem mass spectrometry detection system comprising:

[0006] Liquid chromatograph, which separates the compounds to be tested;

[0007] A mass spectrometer includes an ion source and an exhaust gas discharge device. The inlet of the ion source is connected to the liquid chromatograph. The separated compounds enter the ion source for ionization and generate exhaust gas. The exhaust gas discharge device includes a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel and a turbine component. The first exhaust gas channel has an inlet and an outlet. The exhaust gas enters the inlet of the first exhaust gas channel. The inlet of the heat dissipation component is connected to the outlet of the first exhaust gas channel. The heat dissipation component is used to cool the exhaust gas. The inlet of the second exhaust gas channel is connected to the outlet of the heat dissipation component. The turbine component is arranged at the outlet of the second exhaust gas channel and is used to control the cooled exhaust gas to be discharged through the outlet of the second exhaust gas channel.

[0008] The controller is electrically connected to the liquid chromatograph and the mass spectrometer, respectively, and is used to obtain the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source, confirm the exhaust gas emission rate corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, confirm the target speed corresponding to the turbine assembly according to the exhaust gas emission rate, and control the turbine assembly to operate at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel.

[0009] In some embodiments, the heat dissipation assembly includes: an exhaust box, wherein a plurality of heat dissipation pipes are provided in the exhaust box, the inlet of the exhaust box is connected to the outlet of the first exhaust channel, the outlet of the exhaust box is connected to the inlet of the second exhaust channel, and the extension direction of the heat dissipation pipe is perpendicular to the flow direction of the exhaust gas; an air-driving member, wherein the air-driving member is arranged on one side of the exhaust box, and the air-driving member is used to suck in cold air from the other side of the heat dissipation pipe, so that the exhaust gas is cooled after contacting the pipe wall of the heat dissipation pipe in the exhaust box; a mounting plate, wherein the mounting plate is used to fix the air-driving member on the exhaust box, and the mounting plate includes a plurality of vents, and the vents are arranged corresponding to the heat dissipation pipes; wherein the controller confirms the exhaust gas cooling speed according to the exhaust gas discharge speed, and determines the air-driving power of the air-driving member according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes, so as to control the operation of the air-driving member according to the air-driving power.

[0010] Illustratively, the flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe; and / or the inlet diameter of the second exhaust gas channel is much larger than the outlet diameter of the second exhaust gas channel.

[0011] In some embodiments, the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature, and gradient of the liquid chromatograph.

[0012] In some embodiments, the ion source parameters of the ion source include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure, and heating rod temperature of the ion source.

[0013] In some embodiments, the liquid chromatography-tandem mass spectrometry detection system further includes: a mass analysis detection device, which is connected to the ion source, and the ion source inputs the ions after ionization of the compound into the mass analysis detection device for detection; the controller obtains the reserpine spectrum information and / or triiodothyronine spectrum information corresponding to the ions to update the target speed of the turbine assembly according to the reserpine spectrum information and / or triiodothyronine spectrum information.

[0014] In a second aspect, the present application provides a method for emitting exhaust gas, which is applied to a controller of a liquid chromatography tandem mass spectrometry detection system provided in any embodiment of the present application, and the method comprises:

[0015] Obtaining liquid phase parameters of a liquid chromatograph and ion source parameters of an ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature, and gradient of the liquid chromatograph; and the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure, and heating rod temperature of the ion source;

[0016] Determining an exhaust gas emission velocity corresponding to a mass spectrometer based on the liquid phase parameters and the ion source parameters, and determining a target rotational speed corresponding to a turbine assembly of an exhaust gas emission device based on the exhaust gas emission velocity;

[0017] The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas passage of the exhaust gas discharge device.

[0018] In some embodiments, the method further includes: obtaining reserpine spectrum information of ions detected by a mass analysis detection device; confirming a first peak area corresponding to the mass spectrometer based on the reserpine spectrum information; obtaining a first difference based on the first peak area and the first target peak area, so as to update the target speed based on the first difference; and / or, obtaining triiodothyronine spectrum information of ions detected by a mass analysis detection device; confirming a second peak area corresponding to the mass spectrometer based on the triiodothyronine spectrum information; obtaining a second difference based on the second peak area and the second target peak area, so as to update the target speed based on the second difference.

[0019] In some embodiments, before the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel of the exhaust gas emission device, it also includes: confirming the exhaust gas cooling speed according to the exhaust gas emission speed; determining the wind driving power of the wind driving part of the heat dissipation component according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes of the heat dissipation component; and controlling the operation of the wind driving part of the heat dissipation component according to the wind driving power.

[0020] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further includes: obtaining exhaust gas temperature information at the outlet of the second exhaust gas channel; and adjusting the power of the wind driving component of the heat dissipation assembly according to the exhaust gas temperature information.

[0021] In a third aspect, the present application provides a controller comprising a processor, a memory, and a computer program stored on the memory and executable by the processor, wherein the memory stores a strategy model, wherein when the computer program is executed by the processor, an exhaust gas emission method as provided in any embodiment of the present application is implemented.

[0022] In a fourth aspect, the present application provides a computer-readable storage medium having a computer program stored thereon. When the computer program is executed by a processor, the processor implements the exhaust gas emission method provided in any embodiment of the present application.

[0023] The embodiment of the present application provides a liquid chromatography-tandem mass spectrometry detection system and an exhaust gas emission method. The provided system includes: a mass spectrometer, a liquid chromatograph, and a controller. The mass spectrometer includes an ion source and an exhaust gas emission device. The liquid chromatograph separates the compounds to be detected. The ion source is connected to the liquid chromatograph. The separated compounds enter the ion source for ionization and generate exhaust gas. The exhaust gas emission device includes a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel, and a turbine component. The first exhaust gas channel has an inlet and an outlet. The exhaust gas emitted by the ion source enters the inlet of the first exhaust gas channel. The inlet of the heat dissipation component is connected to the outlet of the first exhaust gas channel. The heat dissipation component is used to cool the exhaust gas. The inlet of the second exhaust gas channel is connected to the outlet of the heat dissipation component. The turbine component is arranged at the outlet of the second exhaust gas channel and is used to control the cooled exhaust gas to be discharged through the outlet of the second exhaust gas channel.

[0024] The controller is electrically connected to the liquid chromatograph and the mass spectrometer, respectively. The provided method uses the controller to obtain the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source. The exhaust gas emission rate corresponding to the mass spectrometer is determined based on the liquid phase parameters and the ion source parameters. The target rotational speed corresponding to the turbine assembly is determined based on the exhaust gas emission rate. The turbine assembly is then controlled to operate at the target rotational speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel. Furthermore, by introducing the exhaust gas emission rate, the target rotational speed corresponding to the turbine assembly is optimized, thereby improving the sensitivity of mass spectrometry detection. This addresses the defect in the prior art that detection sensitivity cannot be optimized by adjusting the exhaust gas emission rate. Different detection objects are matched to different turbine assembly rotational speeds, significantly improving the sensitivity of the mass spectrometer in the liquid chromatography-tandem mass spectrometry detection system.

[0025] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 This is a schematic block diagram of the structure of a liquid chromatography tandem mass spectrometry detection system provided in one embodiment of the present application;

[0028] Figure 2 This is a schematic structural diagram of an exhaust gas emission device provided in one embodiment of the present application;

[0029] Figure 3 This is a schematic flow chart of the steps of the exhaust gas emission method provided in one embodiment of the present application;

[0030] Figure 4 This is a schematic diagram of reserpine profile information provided in one embodiment of the present application;

[0031] Figure 5 This is a schematic diagram of triiodothyronine profile information provided in one embodiment of the present application;

[0032] Figure 6 This is a schematic block diagram of the structure of a controller provided in one embodiment of the present application.

[0033] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0035] The flowcharts shown in the accompanying drawings are for illustrative purposes only and do not necessarily include all contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may be decomposed, combined, or partially merged, so the actual execution order may vary depending on the actual situation.

[0036] It should be understood that the terms used in this specification are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0037] It should be understood that, in order to clearly describe the technical solutions of the embodiments of the present application, in the embodiments of the present application, words such as "first" and "second" are used to distinguish between identical or similar items with substantially the same functions and effects. Those skilled in the art will understand that words such as "first" and "second" do not limit the quantity or execution order, and words such as "first" and "second" do not necessarily mean different.

[0038] It should be further understood that the term “and / or” used in this specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0039] To facilitate understanding of the embodiments of the present application, some of the terms involved in the embodiments of the present application are briefly explained below.

[0040] 1. Liquid chromatography-tandem mass spectrometry detection system: This system tests various indicators in patient blood, body fluids, and other samples collected in clinical laboratories, providing important evidence for disease prevention, diagnosis, treatment plans, and prognosis. Traditional detection methods, such as immunoassays (IA) based on antigen-antibody binding, are routine clinical testing methods. However, clinical practice has shown that IA methods can produce poor selectivity or false-positive results. Liquid chromatography-tandem mass spectrometry (LC-MS / MS) technology, with its high sensitivity and selectivity, provides more accurate analytical results and plays a vital role in clinical testing.

[0041] The ionization modes of mass spectrometry are generally divided into hard ionization and soft ionization. Hard ionization is currently limited to the analysis of volatile and thermally stable compounds and is not suitable for analyzing clinical samples. Soft ionization allows sample molecules to maintain their intact form during the ionization process, which is beneficial for detecting clinical small molecule analytes. Electrospray ionization is the most common ionization mode in clinical mass spectrometry. After the intact sample molecules enter the tandem mass spectrometer, the parent ions of specific compounds are selected in the primary mass spectrometer, and the parent ions are collisionally fragmented in the collision cell. The corresponding ion pair information is then obtained using the secondary mass spectrometer's MRM or SRM mode. Compared to other methods such as ultraviolet spectrophotometry, fluorescence spectroscopy, and immunoassays, tandem mass spectrometry offers advantages such as higher selectivity, accuracy, resolution, and sensitivity. The MRM / SRM mode allows for the accurate simultaneous quantification of multiple analytes.

[0042] LC-MS / MS, based on liquid chromatography separation, is currently the most commonly used detection method in clinical laboratories. Since clinical test samples are typically biological fluids or solids with complex matrices, they are not suitable for direct mass spectrometry analysis and require sample pretreatment and chromatographic separation. Sample preparation during pretreatment can affect not only the chromatographic separation but also the analyte signal intensity. Sometimes, it is necessary to concentrate or dilute the sample based on the detection limit of the LC-MS / MS system being used, and to separate the target from interfering components by optimizing the liquid chromatography conditions.

[0043] 2. Ion source: As the core of the mass spectrometer, the ion source can ionize the organic solution to form gaseous ions and output the gaseous ions to the mass analyzer, so that the mass analyzer can analyze and detect the gaseous ions.

[0044] Taking the electrospray ionization source as an example, after an organic solution is introduced into the electrospray ionization source, it can be ionized under the action of high voltage or high voltage electric field to form an ionic liquid. After the ionic liquid is atomized at high pressure, the solvent can be evaporated in the high-temperature gas to form gaseous ions. However, most of the gaseous ions in the ion source are sucked away by the air pump under the guidance of gravity and airflow. Only a very small number of gaseous ions can be output outside the ion source and transmitted to the mass analyzer. This also leads to a limited ion collision rate in the mass spectrometer and poor sensitivity of the mass spectrometer.

[0045] The following describes some embodiments of the present application in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.

[0046] Liquid chromatography-tandem mass spectrometry (LC-MS / MS) combines the high separation capabilities of liquid chromatography with the highly sensitive and selective detection capabilities of tandem mass spectrometry. Since its introduction, this technology has been widely used in a variety of fields, including life sciences, pharmaceutical research and development, food safety, and environmental monitoring, due to its unique advantages.

[0047] During the use of LC-MS / MS, the sample solution is first separated by liquid chromatography and then enters the ion source for ionization. The charged ions enter the mass spectrometer for detection under the action of potential difference and vacuum difference. The ion source will produce a large amount of waste solvent and sample during the ionization process. These substances reflux in the closed ion source cavity, causing memory effect and contamination of the ion source, thereby affecting the sensitivity of the instrument. Therefore, the exhaust gas of the ion source needs to be discharged and treated.

[0048] Existing ion source exhaust systems mostly use mechanical pumps as exhaust devices. These exhaust structures have a single function and cannot adjust the exhaust speed. Excessively high exhaust speeds can expel some target analytes, preventing them from entering the mass spectrometer, resulting in a low detection signal. Excessively low exhaust speeds prevent effective exhaust removal, leading to instrument contamination and reduced sensitivity. Currently, there is no application of mass spectrometry detection to improve sensitivity by adjusting the exhaust speed.

[0049] Therefore, based on the above content, in order to solve the problem that the waste solvent and sample reflux in the ion source affect the sensitivity of the mass spectrometer, the present application proposes the following Figure 1 The liquid chromatography tandem mass spectrometry detection system 100 is shown. Figure 1 As shown, the liquid chromatography tandem mass spectrometry detection system 100 includes a mass spectrometer 10, a controller 20, and a liquid chromatograph 30. The liquid chromatograph 30 is used to separate compounds to be detected (such as organic compounds). The mass spectrometer 10 includes an exhaust gas exhaust device 11 and an ion source 12. The ion source 12 is connected to the liquid chromatograph 30. The separated compounds enter the ion source 12 for ionization and generate exhaust gas.

[0050] like Figure 2 As shown, the exhaust gas discharge device 11 includes a first exhaust gas channel 111, a heat sink assembly 112, a second exhaust gas channel 113, and a turbine assembly 114. The first exhaust gas channel 111 has an inlet and an outlet. Exhaust gas emitted by the ion source 20 enters the inlet of the first exhaust gas channel 111. The inlet of the heat sink assembly 112 is connected to the outlet of the first exhaust gas channel 111 and is used to cool the exhaust gas. The inlet of the second exhaust gas channel 113 is connected to the outlet of the heat sink assembly 112. The turbine assembly 114 is located at the outlet of the second exhaust gas channel 113 and is used to control the cooled exhaust gas (or liquid formed by condensation of the exhaust gas) to be discharged through the outlet of the second exhaust gas channel 113.

[0051] The controller 20 is electrically connected to the liquid chromatograph 30 and the mass spectrometer 10, respectively. The controller 20 is used to obtain the liquid phase parameters of the liquid chromatograph 30 and the ion source parameters of the ion source 12, determine the exhaust gas emission rate corresponding to the mass spectrometer 10 based on the liquid phase parameters and the ion source parameters, determine the target speed corresponding to the turbine assembly 114 based on the exhaust gas emission rate, and control the turbine assembly 114 to operate at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel.

[0052] Furthermore, the provided method can adjust the rotational speed of the turbine assembly 114, enabling the mass spectrometer to accurately and efficiently discharge the exhaust gas generated by ionization of the ion source 12. This improves the detection efficiency and sensitivity of the mass spectrometer 10. Furthermore, different detection objects can be matched to different rotational speeds of the turbine assembly 114, significantly enhancing the sensitivity of the mass spectrometer.

[0053] It should be noted that the present application can discharge hot exhaust gas generated by various types of mass spectrometers 10 , and the present application takes a liquid chromatography tandem mass spectrometer as an example for explanation.

[0054] In some embodiments, as Figure 2 As shown, the heat dissipation assembly 112 includes an exhaust box 1121, a heat pipe 1122, a wind-dispelling member 1123, and a mounting plate 1124. Multiple heat pipes 1122 are located within the exhaust box 1121. The inlet of the exhaust box 1121 is connected to the outlet of the first exhaust duct 111, while the outlet of the exhaust box 1121 is connected to the inlet of the second exhaust duct 113. The heat pipes 1122 extend perpendicular to the flow of exhaust gas. The wind-dispelling member 1123 is located on one side of the exhaust box 1121 and is used to draw cool air from the other side of the heat pipe 1122, cooling the exhaust gas within the exhaust box 1121 upon contact with the walls of the heat pipe 1122. The mounting plate 1124 is used to securely attach the wind-dispelling member 1123 to the exhaust box 1121. The mounting plate 1124 includes multiple ventilation holes corresponding to the heat pipes 1122.

[0055] Controller 20 determines the exhaust cooling rate based on the exhaust gas emission rate, and determines the wind driving power of wind driving element 1123 based on the exhaust gas cooling rate and the number and size of the heat pipes, thereby controlling the operation of wind driving element 1123 according to the wind driving power. The provided method can simultaneously control the turbine assembly 114 and the exhaust gas emission rate to control the power of wind driving element 1123, thereby improving the efficiency of exhaust gas emission.

[0056] For example, Figure 2 As shown, the wind-repelling member 1123 is a cooling fan, which can be installed on any opening side of the heat pipe 1122 and can suck in the cold air on the other side to achieve a cooling effect.

[0057] Exemplarily, the heat dissipation pipe 1122 is made of a heat-conducting material, such as aluminum or copper, so that the exhaust gas can contact the side wall of the heat dissipation pipe 1122 to be cooled.

[0058] For example, the flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe. This can avoid the turbine assembly 114 from contacting the heat dissipation assembly 112 (e.g. Figure 2 The turbine assembly 114 and the wind driving member 12 will not be in the same direction).

[0059] For example, the inlet diameter of the second exhaust passage is much larger than the outlet diameter of the second exhaust passage, thereby improving the exhaust emission efficiency of the turbine assembly 114 .

[0060] In some embodiments, the turbine assembly 114 is a turbo blower, and the exhaust gas emission rate of the mass spectrometer 10 can be controlled by controlling the rotation speed of the turbo blower.

[0061] In some embodiments, the liquid phase parameters of the liquid chromatograph 30 include at least one or more of the mobile phase composition, flow rate, column temperature, and gradient of the liquid chromatograph 30. Examples of mobile phase composition include: mobile phase A: 0.1% formic acid in water, mobile phase B: 0.1% formic acid in acetonitrile. The column temperature includes the temperature of a chromatographic column, such as a C18 column (2.1*30 mm, 1.3 μm). Liquid phase parameters may also include, for example, injection volume. The corresponding exhaust gas emission rate can then be determined based on the current liquid phase parameters.

[0062] In some embodiments, the ion source parameters of the ion source 12 include at least one or more of the ion source voltage, nebulizer gas pressure, auxiliary heater gas pressure, and heater rod temperature of the ion source 12. Ion source parameters, also referred to as mass spectrometry conditions, may also include parameters such as entrance voltage, collision voltage, and potential difference to improve optimization efficiency for exhaust gas emission velocity and target rotational speed of the turbine assembly 114 in combination with ion source information from the mass spectrometer.

[0063] In some embodiments, the provided liquid chromatography tandem mass spectrometry detection system 100 also includes: a mass analysis detection device (such as a mass analyzer and a mass detector), the mass analysis detection device is connected to the ion source 12, and the ion source 12 inputs the ions after ionization of the compound into the mass analysis detection device for detection; the controller 20 obtains the reserpine spectrum information and / or triiodothyronine (T3) spectrum information corresponding to the ions to update the target speed of the turbine assembly according to the reserpine spectrum information and / or triiodothyronine spectrum information.

[0064] In some embodiments, the controller 10 may be a microcontroller unit (MCU), or an external terminal device such as a computer device, a server, a tablet, etc. The embodiment of the present application does not limit the type of the controller 10 .

[0065] The present invention provides a liquid chromatography-tandem mass spectrometry detection system. By incorporating the exhaust gas velocity of the mass spectrometer into the system, the target rotational speed of the turbine assembly is optimized, thereby improving mass spectrometry detection sensitivity. This overcomes the existing limitation of the inability to optimize detection sensitivity by adjusting the exhaust gas velocity. Furthermore, different turbine assembly rotational speeds are matched to different test objects, significantly improving the sensitivity of the mass spectrometer in the system.

[0066] See also Figure 3 , the embodiment of the present application proposes a method for exhaust gas emission, and the provided method is applied to the controller of the liquid chromatography tandem mass spectrometry detection system provided in any embodiment of the present application. Figure 3 As shown, the provided method includes steps S201 to S203.

[0067] S201. Obtain the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph; the ion source parameters include at least one or more of the ion source voltage, nebulizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source.

[0068] S202. Determine the exhaust gas emission speed corresponding to the mass spectrometer based on the liquid phase parameters and the ion source parameters, and determine the target rotation speed corresponding to the turbine assembly of the exhaust gas emission device based on the exhaust gas emission speed.

[0069] S203. Control the turbine device to operate according to the target speed, and discharge the cooled exhaust gas through the outlet of the second exhaust gas channel of the exhaust gas emission device.

[0070] The controller then obtains the liquid phase parameters of the mass spectrometer and the ion source parameters of the ion source, and can determine the corresponding exhaust gas emission rate using a preset neural network model (such as a convolutional neural network model), or determine the corresponding exhaust gas emission rate from a preset mapping relationship between the liquid phase parameters, ion source parameters, and exhaust gas emission rates. The target speed of the turbine assembly is then determined based on the exhaust gas emission rate to improve the sensitivity of the mass spectrometer.

[0071] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further includes: obtaining reserpine spectrum information of the ions detected by the mass analysis detection device; determining a first peak area corresponding to the mass spectrometer based on the reserpine spectrum information; obtaining a first difference between the first peak area and a first target peak area, and updating the target speed based on the first difference. The target speed of the turbine assembly can then be optimized and updated based on the peak area corresponding to the reserpine spectrum information.

[0072] For example, without changing the liquid phase parameters and ion source parameters, when monitoring the reserpine quantitative ion (609.3→195.1), when manually adjusting the speed of the turbine assembly, such as Figure 4 As shown ( Figure 4 (a) Figure 4 (b) Figure 4 (c) Figure 4 In (d), the blue curve represents the reserpine spectrum of the reserpine quantification ion (609.3→195.1), and the remaining gray, red, and green colors represent the reserpine spectra of the remaining auxiliary ion pairs, respectively. Figure 4 (a) is the reserpine spectrum when the turbine assembly speed is manually adjusted to 20460 r / min; Figure 4 (b) is the reserpine spectrum with the turbine assembly speed manually adjusted to 41400 r / min; Figure 4 (c) is the reserpine spectrum when the turbine assembly speed is manually adjusted to 46020 r / min. Figure 4 In (a)(b)(c), the observation time is 2.35s. Figure 4 (a) Figure 4 (b) and Figure 4 The peak areas of the blue curves in (c) are 16816.8, 22606.5, and 22318.8, respectively.

[0073] The target speed obtained by the provided method is 28860r / min after optimization. Figure 4 (d) is the spectrum of reserpine when the turbine assembly speed is adjusted to 28860 r / min using the provided method. It can be seen that under the same conditions, the peak area of ​​the blue curve is 25214.7. Figure 4 The vertical coordinates of the reserpine maps can be seen in Figure 4 The vertical coordinate of the blue curve in (d) is the strongest intensity value.

[0074] As can be seen, after optimization, the target speed of 28,860 r / min yielded the highest peak area of ​​25,214.7 for the reserpine quantification ion (609.3 → 195.1), compared to the peak area of ​​16,816.8 at a speed of 20,460 r / min, representing a nearly 50% increase. This demonstrates that the method provided in this application can rapidly determine the target speed of the turbine assembly corresponding to the liquid phase parameters and ion source parameters.

[0075] In some embodiments, triiodothyronine spectrum information of ions detected by a mass analysis detection device is obtained; a second peak area corresponding to the mass spectrometer is determined based on the triiodothyronine spectrum information; a second difference is obtained based on the second peak area and a second target peak area, and the target speed is updated based on the second difference. The target speed of the turbine assembly can then be optimized and updated based on the peak area corresponding to the triiodothyronine spectrum information.

[0076] For example, without changing the liquid phase parameters and ion source parameters, when monitoring the triiodothyronine quantitative ion (652.0→605.8), when manually adjusting the speed of the turbine assembly, such as Figure 5 As shown ( Figure 5 (a) Figure 5 (b) Figure 5 (c) Figure 5 The blue curve in (d) represents the triiodothyronine spectrum of the triiodothyronine quantitative ion (652.0→605.8), and the remaining gray, red, and green colors represent the triiodothyronine spectra of the remaining auxiliary ion pairs, respectively. Figure 5 (a) is the triiodothyronine spectrum when the turbine assembly speed is manually adjusted to 16560 r / min; Figure 5 (b) is the triiodothyronine spectrum when the turbine assembly speed is manually adjusted to 25260 r / min; Figure 5 (c) is the spectrum of triiodothyronine when the turbine assembly speed is manually adjusted to 34860 ​​r / min. Figure 5 In (a), (b), and (c), the unified observation time is 3.69s. Figure 5 (a) Figure 5 (b) and Figure 5 The peak areas of the blue curves in (c) are 37464, 39282, and 46360, respectively (the blue curve represents the triiodothyronine quantitative ion (652.0→605.8), and the red curve represents the triiodothyronine spectrum of the auxiliary ion pair).

[0077] The target speed obtained by the provided method is 45660r / min after optimization. Figure 5 (d) is the triiodothyronine spectrum information when the turbine assembly speed is adjusted to 45660 r / min using the provided method. It can be seen that under the same conditions, the peak area of ​​the blue curve is 51062. Figure 5 The vertical coordinates of the triiodothyronine spectra can be seen in Figure 5 The vertical coordinate of the blue curve in (d) is the strongest intensity value.

[0078] As can be seen, after optimization, the target speed of 45,660 r / min was obtained, and the peak area of ​​the triiodothyronine quantitative ion (652.0→605.8) reached the highest level, 51,062, compared to the peak area of ​​37,464 at a speed of 16,560 r / min, a 36.3% increase. This means that the method provided in this application can quickly determine the target speed of the turbine assembly corresponding to the liquid phase parameters and ion source parameters, thereby obtaining the optimal results for each indicator.

[0079] In some embodiments, before the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel of the exhaust gas emission equipment, it also includes: confirming the exhaust gas cooling speed according to the exhaust gas emission speed; determining the wind driving power of the wind driving part of the heat dissipation component according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes of the heat dissipation component; and controlling the operation of the wind driving part of the heat dissipation component according to the wind driving power.

[0080] Furthermore, the provided method can quickly determine the air driving power of the air driving component (such as a cooling fan) required to achieve the expected exhaust gas cooling rate based on the number and size of the heat pipes, ensuring that the air driving component can meet the heat dissipation requirements of the mass spectrometer.

[0081] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further includes: obtaining exhaust gas temperature information at the outlet of the second exhaust gas channel; and adjusting the power of the wind driving component of the heat dissipation assembly according to the exhaust gas temperature information.

[0082] When the second exhaust gas channel discharges the exhaust gas or the exhaust liquid formed by the condensation of the exhaust gas, the power of the wind driving member is adjusted if the temperature of the exhaust liquid is too high by detecting the temperature of the exhaust liquid.

[0083] The present invention provides an exhaust gas emission method that optimizes the target rotational speed of the turbine assembly by incorporating the exhaust gas emission velocity of a mass spectrometer, thereby improving mass spectrometer detection sensitivity. This method addresses the existing limitation of the inability to optimize detection sensitivity by adjusting the exhaust gas emission velocity. Furthermore, different turbine assembly rotational speeds are matched to different detection objects, significantly improving the sensitivity of the mass spectrometer.

[0084] See also Figure 6 , Figure 6 This is a schematic block diagram of a controller provided in an embodiment of the present application. The controller may be a server. Figure 6 The controller includes a processor, a memory, and a network interface connected via a system bus, wherein the memory may include a non-volatile storage medium and an internal memory.

[0085] The non-volatile storage medium can store an operating system and a computer program. The computer program includes program instructions, and when the program instructions are executed, the processor can execute any one of the exhaust gas emission methods.

[0086] The processor is used to provide computing and control capabilities and support the operation of the entire controller.

[0087] The internal memory provides an environment for the operation of the computer program in the non-volatile storage medium. When the computer program is executed by the processor, the processor can execute any exhaust gas emission method.

[0088] The network interface is used for network communication, such as sending assigned tasks, etc. Those skilled in the art will understand that Figure 6 The structure shown in the figure is only a block diagram of a part of the structure related to the solution of the present application, and does not constitute a limitation on the controller to which the solution of the present application is applied. The specific controller may include more or fewer components than shown in the figure, or combine certain components, or have a different component arrangement.

[0089] It should be understood that the processor may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor may be a microprocessor or any conventional processor, etc.

[0090] In one embodiment, the processor is configured to execute a computer program stored in the memory to implement the following steps:

[0091] Obtaining liquid phase parameters of a liquid chromatograph and ion source parameters of an ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature, and gradient of the liquid chromatograph; and the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure, and heating rod temperature of the ion source;

[0092] Determine the exhaust gas emission velocity corresponding to the mass spectrometer based on the liquid phase parameters and the ion source parameters, and determine the target speed corresponding to the turbine assembly of the exhaust gas emission equipment based on the exhaust gas emission velocity;

[0093] The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas passage of the exhaust gas discharge device.

[0094] A computer-readable storage medium is also provided in an embodiment of the present application. The computer-readable storage medium stores a computer program. The computer program includes program instructions. The processor executes the program instructions to implement any one of the exhaust gas emission methods provided in the embodiments of the present application.

[0095] The computer-readable storage medium may be an internal storage unit of the controller described in the aforementioned embodiment, such as a hard disk or memory of the controller. The computer-readable storage medium may also be an external storage device of the controller, such as a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash memory card, etc., equipped on the controller.

[0096] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present application, and such modifications or substitutions should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. A liquid chromatography tandem mass spectrometry detection system, characterized in that: The liquid chromatography tandem mass spectrometry detection system comprises: a liquid chromatograph, wherein the liquid chromatograph separates the compounds to be detected; A mass spectrometer, the mass spectrometer comprising an ion source and an exhaust gas discharge device, the inlet of the ion source being connected to a liquid chromatograph, the separated compounds entering the ion source for ionization and generating exhaust gas, the exhaust gas discharge device comprising a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel, and a turbine component; the first exhaust gas channel having an inlet and an outlet, the exhaust gas entering the inlet of the first exhaust gas channel; the inlet of the heat dissipation component being connected to the outlet of the first exhaust gas channel, the heat dissipation component being used to cool the exhaust gas; the inlet of the second exhaust gas channel being connected to the outlet of the heat dissipation component; the turbine component being disposed at the outlet of the second exhaust gas channel, and being used to control the cooled exhaust gas to be discharged through the outlet of the second exhaust gas channel; a controller, the controller being electrically connected to the liquid chromatograph and the mass spectrometer, respectively, and configured to obtain liquid phase parameters of the liquid chromatograph and ion source parameters of the ion source, determine an exhaust gas emission velocity corresponding to the mass spectrometer based on the liquid phase parameters and the ion source parameters, determine a target speed corresponding to the turbine assembly based on the exhaust gas emission velocity, and control the turbine assembly to operate at the target speed to discharge the cooled exhaust gas through an outlet of the second exhaust gas channel; The heat dissipation assembly includes: an exhaust box, wherein a plurality of heat pipes are provided in the exhaust box, the inlet of the exhaust box is connected to the outlet of the first exhaust channel, the outlet of the exhaust box is connected to the inlet of the second exhaust channel, and the extension direction of the heat pipe is perpendicular to the flow direction of the exhaust gas; an air drive member, the air drive member is arranged on one side of the exhaust box, and the air drive member is used to suck cold air from the other side of the heat pipe, so that the exhaust gas is cooled after contacting the pipe wall of the heat pipe in the exhaust box; a mounting plate, the mounting plate is used to fix the air drive member on the exhaust box, and the mounting plate includes a plurality of vents, and the vents are arranged corresponding to the heat pipes; wherein the controller determines the exhaust gas cooling speed according to the exhaust gas discharge speed, determines the air drive power of the air drive member according to the exhaust gas cooling speed and the number and size of the heat pipes, so as to control the operation of the air drive member according to the air drive power; the inlet diameter of the second exhaust channel is much larger than the outlet diameter of the second exhaust channel; The system further includes: a mass analysis and detection device, the mass analysis and detection device being connected to an ion source, the ion source inputting ions ionized from the compound into the mass analysis and detection device for detection; a controller acquiring reserpine spectrum information and / or triiodothyronine spectrum information corresponding to the ions, and updating a target speed of the turbine assembly based on the reserpine spectrum information and / or triiodothyronine spectrum information; By obtaining reserpine spectrum information of ions detected by a mass analysis detection device; confirming a first peak area corresponding to the mass spectrometer based on the reserpine spectrum information; obtaining a first difference based on the first peak area and a first target peak area, so as to update the target speed based on the first difference; and / or, obtaining triiodothyronine spectrum information of ions detected by a mass analysis detection device; confirming a second peak area corresponding to the mass spectrometer based on the triiodothyronine spectrum information; obtaining a second difference based on the second peak area and a second target peak area, so as to update the target speed based on the second difference.

2. The liquid chromatography tandem mass spectrometry detection system according to claim 1, characterized in that: The flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe.

3. The liquid chromatography tandem mass spectrometry detection system according to claim 1, characterized in that: The liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph.

4. The liquid chromatography tandem mass spectrometry detection system according to claim 1, characterized in that: The ion source parameters of the ion source include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source.

5. A method for exhaust gas emission, characterized in that: A controller for a liquid chromatography tandem mass spectrometry detection system according to any one of claims 1 to 4, the method comprising: Obtaining liquid phase parameters of a liquid chromatograph and ion source parameters of an ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature, and gradient of the liquid chromatograph; and the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure, and heating rod temperature of the ion source; Determining an exhaust gas emission velocity corresponding to a mass spectrometer based on the liquid phase parameters and the ion source parameters, and determining a target rotational speed corresponding to a turbine assembly of an exhaust gas emission device based on the exhaust gas emission velocity; The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel of the exhaust gas emission equipment; the method also includes: obtaining reserpine spectrum information of the ions detected by the mass analysis detection device; confirming the first peak area corresponding to the mass spectrometer according to the reserpine spectrum information; obtaining a first difference according to the first peak area and the first target peak area, so as to update the target speed according to the first difference; and / or, obtaining triiodothyronine spectrum information of the ions detected by the mass analysis detection device and confirming the second peak area corresponding to the mass spectrometer according to the triiodothyronine spectrum information; obtaining a second difference according to the second peak area and the second target peak area, so as to update the target speed according to the second difference.

6. The method according to claim 5, characterized in that Before discharging the cooled exhaust gas through the outlet of the second exhaust gas channel of the exhaust gas discharge device, the method further includes: Determining the exhaust gas cooling rate based on the exhaust gas emission rate; Determine the wind driving power of the wind driving member of the heat dissipation assembly according to the exhaust gas cooling rate and the number and size of the heat dissipation pipes of the heat dissipation assembly; The operation of the wind-driving component of the heat dissipation component is controlled according to the wind-driving power.

7. The method according to claim 5, characterized in that After the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further includes: acquiring exhaust gas temperature information at an outlet of the second exhaust gas channel; The power of the wind driving part of the heat dissipation component is adjusted according to the exhaust gas temperature information.

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