Composition containing probiotics as well as preparation method and application thereof in weight loss

Abstract

The invention belongs to the technical field of probiotics, and provides a composition containing probiotics as well as a preparation method and application thereof in weight loss, the composition containing probiotics comprises the following raw materials in parts by weight: 15-25 parts of compound probiotics, 20-35 parts of plant active ingredients, 10-20 parts of dietary fibers and 2-5 parts of a flavoring agent, wherein the plant active component is a composition of baicalin nanoparticles and a eucommia ulmoides extract. According to the composition containing the probiotics, the composite probiotics and the plant active ingredients are scientifically and reasonably matched for use, the ingredients are natural and high in safety, the variety and number of the probiotics in gastrointestinal tracts can be increased, all the ingredients are synergistic, the weight losing and fat reducing effects are good, meanwhile, the composition is good in taste and strong in satiety, diuresis is achieved, bowel relaxing is achieved, and the composition is suitable for being used in the gastrointestinal tracts. Good market values are realized.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotics, and particularly relates to a composition comprising probiotics, a preparation method thereof, and an application of the composition in weight loss. Background Art

[0002] As the pace of life continues to accelerate, obesity has become a global social problem due to work pressure, staying up late, lack of exercise, diet and other reasons. Obesity not only affects personal appearance, but is also closely related to a variety of metabolic diseases such as hyperlipidemia, hypertension, diabetes, etc., posing a serious threat to human health. Traditional weight loss methods, such as dieting and drug treatment, may have problems such as ineffectiveness or side effects.

[0003] Obesity is mainly caused by excessive accumulation of adipose tissue due to imbalance between energy intake and consumption. Studies in recent years have shown that intestinal microorganisms are closely related to obesity. Probiotics can improve lipid metabolism and reduce fat accumulation in the body, thus playing a preventive and therapeutic role in obesity and its related diseases.

[0004] At present, most products scientifically mix probiotics and plant active ingredients, which can not only improve the activity of probiotics in the gastrointestinal tract, but also effectively regulate the intestinal flora, increase the types and number of probiotics, and inhibit the growth of harmful bacteria, thereby achieving the effect of weight loss and fat reduction. However, although most probiotics have a weak lipid-lowering and blood sugar-lowering effect, the effect is very limited, and a reasonable ratio is needed to better regulate the intestinal flora; at the same time, how plant active ingredients can effectively exert their lipid-lowering effect also needs further research.

[0005] Chinese patent CN 114558038B discloses a probiotic composition for weight loss and fat reduction, and a preparation method and application thereof. The probiotic composition for weight loss and fat reduction comprises the following components: composite probiotic powder, plant active ingredients, auxiliary protective agents, fillers, and sweeteners. The probiotic composition provided by this invention can effectively reduce the formation of fat in the body, and at the same time can increase the types and quantity of probiotics, inhibit the growth of harmful bacteria, and improve the stability and weight loss effect of the probiotic composition. The weight loss effect is significant and suitable for a wide range of people. However, the composition has diverse ingredients, a complex preparation process, and a high production cost.

[0006] Therefore, there is an urgent need for a composition containing probiotics, which has simple ingredients and can effectively improve the effect of weight loss and fat reduction. Summary of the invention

[0007] In view of the existing technical problems, the purpose of the present invention is to provide a composition containing probiotics, a preparation method thereof and an application thereof in weight loss. The composition containing probiotics of the present invention scientifically and rationally combines composite probiotics and plant active ingredients, has natural ingredients and high safety, can not only increase the types and quantity of probiotics in the gastrointestinal tract, but also has synergistic effects between the ingredients, has good weight loss and fat reduction effects, and at the same time, the composition has good taste, strong satiety, diuresis and laxative effect, and has good market value.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] The present invention first provides a composition containing probiotics, which comprises the following raw materials by weight: 15-25 parts of composite probiotics, 20-35 parts of plant active ingredients, 10-20 parts of dietary fiber, and 2-5 parts of flavoring agents; wherein the plant active ingredients are a composition of baicalin nanoparticles and eucommia ulmoides extract.

[0010] The reaction mechanism and effects of the present invention are as follows:

[0011] 1. The present application uses papain and acid protease to enzymatically hydrolyze corn protein powder to form corn oligopeptides. The high glutamine content in corn oligopeptides is closely related to the growth and repair of human tissues, which can improve the body's immunity and ability to adapt to external harmful stimuli; at the same time, corn oligopeptides have anti-fatigue properties, which can increase muscle glycogen content and liver glycogen content, enhance the body's exercise endurance, and are beneficial to people who need to control their weight through exercise.

[0012] Baicalin, a flavonoid extracted from the traditional Chinese medicine Scutellaria baicalensis, has the effects of lowering blood lipids and reducing weight. Baicalin can inhibit the differentiation of 3T3-L1 preadipocytes into adipocytes, and inhibit the accumulation of triglycerides and the formation of lipid droplets in adipocytes. It can also inhibit lipogenesis through the Wnt / β-Catenin pathway. Baicalin can directly activate carnitine palmitoyltransferase 1 (CPT1A) in the liver, thereby accelerating the β-oxidation process of fatty acids and promoting fat consumption and decomposition; in addition, baicalin can effectively regulate and restore the abundance of intestinal flora (mainly Firmicutes and Bacteroidetes) and the production of short-chain fatty acids (mainly acetic acid) in the high-fat diet group, thereby affecting energy intake, storage and consumption. However, baicalin has poor solubility in water and an oral bioavailability of only 2.2%, which limits its application in weight loss.

[0013] The present invention utilizes the deprotonation of hydroxyl groups of baicalin in an alkaline environment to improve the solubility, and then loads it on corn oligopeptides to prepare baicalin nanoparticles, which not only greatly improve the dispersibility, stability and bioavailability of baicalin, but also significantly improve the weight loss effect.

[0014] 2. Eucommia ulmoides has a sweet and warm nature, and its main chemical components are lignans, iridoids, flavonoids, phenylpropanoids and terpenes. Modern pharmacological studies have shown that Eucommia ulmoides has antioxidant, hypoglycemic, hypolipidemic, diuretic and laxative, and immunomodulatory effects.

[0015] The present invention uses a composite enzyme to first enzymolyze the eucommia ulmoides and then ferments it with composite bacteria, which can protect the effective components of the eucommia ulmoides from being destroyed to the greatest extent, reduce the dosage and improve the efficacy of the drug, and then the eucommia ulmoides extract obtained after alcohol extraction shows better effects in diuresis and laxative, which is conducive to the discharge of toxins in the body. The applicant controls the type and proportion of the composite bacteria to make the effective components easier to release, thereby improving the therapeutic effect of the eucommia ulmoides extract.

[0016] 3. The present invention adds dietary fiber with low energy value, which can be fermented to produce short-chain fatty acids under the action of intestinal bacteria, thereby stimulating the activity of probiotics and maintaining the health of the intestinal environment; at the same time, dietary fiber can cause swelling in the stomach by absorbing water and certain lipids, producing a sense of fullness, thereby effectively controlling appetite and reducing food intake; in addition, dietary fiber can promote intestinal peristalsis, increase bowel movement frequency and water intake, and help maintain the smooth operation of the digestive system.

[0017] 4. The flavoring agent added in the present invention improves the taste of the composition, has good palatability, and improves the compliance of taking the product, which not only makes the product easier to accept, but also helps users to take it for a long time, thereby ensuring the continuity and stability of the therapeutic effect. While ensuring the health benefits, it also satisfies consumers' pursuit of taste and achieves a perfect combination of health and deliciousness.

[0018] In some embodiments, the composite probiotic is a combination of Bacillus subtilis, Lactobacillus rhamnosus, Lactobacillus acidophilus and Bifidobacterium longum.

[0019] Preferably, the mass ratio of the Bacillus subtilis, Lactobacillus rhamnosus, Lactobacillus acidophilus and Bifidobacterium longum is (0.8-1.4):1:1:(1-1.8).

[0020] Preferably, the Bacillus subtilis is deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration, with a deposit number of CGMCC No. 20831, a classification name of Bacillus subtilis-1, a deposit date of September 25, 2020, and a deposit address of No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0021] In some embodiments, the plant active ingredients include the following raw materials by weight: 15-25 parts of baicalin nanoparticles and 5-10 parts of Eucommia ulmoides extract.

[0022] In some embodiments, the method for preparing baicalin nanoparticles comprises the following steps:

[0023] S1. adding baicalin to an alkaline solution, heating and stirring, and cooling to form a baicalin alkaline solution;

[0024] S2. Disperse corn gluten powder in water, stir, add papain, and hydrolyze for 2-3 hours at a temperature of 50-60°C and a pH of 5-7, then adjust the pH to 3-4 and the temperature to 40-45°C, add acidic protease for hydrolysis for 2-3 hours, inactivate the enzyme, and filter to obtain corn oligopeptide;

[0025] S3. The baicalin alkali solution obtained in step S1 and the corn oligopeptide obtained in step S2 are mixed, the pH value is adjusted to 10-12, stirred for 2-3 hours, 10%-20% by mass fraction of glucose-δ-lactone aqueous solution is added to adjust the pH value to 5-6.5, freeze-dried, and ground to obtain baicalin nanoparticles.

[0026] In some embodiments, the concentration of the alkaline solution in step S1 is 3-5 mol / L, and the mass fraction of baicalin alkaline solution is 8%-12%.

[0027] In some embodiments, the total amount of papain and acid protease in step S2 is 1%-2% of the mass of corn gluten meal.

[0028] Preferably, the mass ratio of papain to acid protease in step S2 is 1.25:1.

[0029] In some embodiments, the mass ratio of baicalin to corn oligopeptide in the baicalin alkali solution in step S3 is 1:(3-6).

[0030] In some embodiments, the method for preparing the Eucommia ulmoides extract comprises the following steps:

[0031] A1. Wash the Eucommia ulmoides, dry it, grind it into powder, stir it with water, add a complex enzyme to hydrolyze it, and obtain an enzymatic solution;

[0032] A2. A 3wt%-6wt% aqueous solution of glucose was added to the enzymatic solution obtained in step A1, sterilized, added with composite bacteria for fermentation, sterilized, and obtained a fermentation product;

[0033] A3. The fermentation product obtained in step A2 is centrifuged, filtered, dried, ultrasonically extracted 2-3 times with 50wt%-70wt% ethanol, centrifuged, filtered, membrane concentrated, and spray-dried to obtain an eucommia extract.

[0034] In some embodiments, the composite bacteria in step A2 are Neurospora robusta and Lactobacillus plantarum, and the mass ratio of the two is 1:(1.5-2.5).

[0035] In some embodiments, the complex enzyme in step A1 is aspartic protease and cellulase, and the mass ratio of the two is 1:(1.2-2).

[0036] In some embodiments, the flavoring agent is any one or more of erythritol, isomalt, maltitol, sorbitol and lactitol.

[0037] In some embodiments, the dietary fiber is any one or more of inulin, psyllium husk powder, konjac flour, soybean fiber, apple fiber, oat fiber and rice bran.

[0038] The present invention also provides a method for preparing the composition containing probiotics, comprising the following steps:

[0039] The composite probiotics, plant active ingredients, dietary fiber and flavoring agents are mixed and stirred to obtain a composition containing probiotics.

[0040] The third aspect of the present invention provides the use of the composition containing probiotics in weight loss, wherein the composition is in the form of granules, powders, tablets or capsules.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] 1. The composition containing probiotics of the present invention scientifically and rationally combines composite probiotics and plant active ingredients for use, has natural ingredients and high safety, can not only increase the types and quantities of probiotics in the gastrointestinal tract, but also the synergistic effect between the ingredients, and has a good weight loss and fat reduction effect. At the same time, the composition has a good taste, a strong sense of fullness, and is diuretic and laxative, and has a good market value.

[0043] 2. The present application uses papain and acid protease to enzymatically hydrolyze corn protein powder in succession to form corn oligopeptides, and then utilizes the deprotonation of hydroxyl groups of baicalin in an alkaline environment to improve the solubility, and then loads it on the corn oligopeptides to prepare baicalin nanoparticles, thereby improving the dispersibility, stability and bioavailability of baicalin, thereby significantly improving the weight loss effect.

[0044] 3. The present invention uses a composite enzyme to first enzymolyze the Eucommia ulmoides and then ferments it with a composite bacteria. The Eucommia ulmoides extract obtained after alcohol extraction shows better effects in diuresis and laxative. DETAILED DESCRIPTION

[0045] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, rather than to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0046] According to the ratios of the raw materials and the preparation methods specified in the following examples and comparative examples, a composition containing probiotics was prepared.

[0047] In order to facilitate those skilled in the art to implement the present invention, the sources of some raw materials in the embodiments and comparative examples are described as follows:

[0048] Lactobacillus rhamnosus and Bifidobacterium longum were purchased from Hebei Liqia Biotechnology Co., Ltd.

[0049] Lactobacillus acidophilus and papain were purchased from Gansu Yishengxiang Biotechnology Co., Ltd.

[0050] Neurospora robusta: purchased from Shanghai Lianzu Biotechnology Co., Ltd.;

[0051] Lactobacillus plantarum: purchased from Weikang Probiotics (Suzhou) Co., Ltd.;

[0052] Aspartic protease, cellulase, and acidic protease were purchased from Hebei Hongtao Bioengineering Co., Ltd.

[0053] Eucommia ulmoides extract: purchased from Guangzhou Huayu Biotechnology Co., Ltd.;

[0054] Baicalin: purchased from Shaanxi New Horizon Biotechnology Co., Ltd.;

[0055] Other raw materials are not specially specified and can be purchased from the market.

[0056] Preparation Example 1

[0057] The preparation method of baicalin nanoparticles A comprises the following steps:

[0058] S1. 10 g of baicalin was added to 100 ml of a 4 mol / L aqueous sodium hydroxide solution, heated to 85 ° C and stirred for 5 h, and cooled to room temperature to form a baicalin alkali solution;

[0059] S2. 18g corn protein powder was dispersed in 180ml water, stirred at a rate of 1500r / min for 30min, 0.15g papain was added, and the enzymatic hydrolysis was carried out at a temperature of 55°C and a pH value of 6 for 2.5h, and then the pH value was adjusted to 3 and the temperature was 40°C, and 0.12g acid protease was added for enzymatic hydrolysis for 3h, the enzyme was inactivated, and filtered to obtain corn oligopeptide;

[0060] S3. Mix 30 ml of baicalin alkali solution obtained in step S1 and 13.5 g of corn oligopeptide obtained in step S2, adjust the pH value to 11, stir for 3 hours, add the currently prepared 15% mass fraction of glucose-δ-lactone aqueous solution, adjust the pH value to 6, heat from -10°C to 25°C over 8 hours, dry for 4 hours, grind, seal and store to obtain baicalin nanoparticles A.

[0061] Preparation Example 2

[0062] The preparation method of baicalin nanoparticles B is the same as that of Preparation Example 1, except that the amount of papain added in step S2 is 0.08 g, and the amount of acid protease added is 0.064 g.

[0063] Preparation Example 3

[0064] The preparation method of baicalin nanoparticles C is the same as that of Preparation Example 1, except that the amount of baicalin alkali solution added in step S3 is 48 ml.

[0065] Preparation Example 4

[0066] The preparation method of Eucommia ulmoides extract A comprises the following steps:

[0067] A1. Wash 1 kg of Eucommia ulmoides, dry at 60°C for 6 hours, grind into powder, add 10 L of water and stir for 30 minutes, add 5 g of aspartic protease and 8 g of cellulase, adjust the pH to 5, enzymolyze at 50°C for 2.5 hours, and then boil for 5 minutes to inactivate the enzyme to obtain an enzymolysis solution;

[0068] A2. 0.2 kg of 5 wt% aqueous glucose solution was added to the enzymatic solution obtained in step A1, sterilized at 80 ° C for 10 min, 25 g of Neurospora robusta, 50 g of Lactobacillus plantarum were added, fermented at 30 ° C for 24 h, and sterilized at 80 ° C for 10 min to obtain a fermentation product;

[0069] A3. The fermentation product obtained in step A2 was centrifuged, filtered, and dried at 90°C to constant weight. The product was extracted by ultrasonic wave for 3 times with 60wt% ethanol, which was 10 times the amount of the dried product, for 30 min each time. The product was centrifuged, filtered, and then filtered with a 0.22μm microporous filter membrane. The product was spray dried for 15s. The inlet air temperature was controlled at 145°C and the outlet air temperature was controlled at 75°C to obtain Eucommia ulmoides extract A.

[0070] Preparation Example 5

[0071] The preparation method of Eucommia ulmoides extract B is the same as that of Preparation Example 1, except that 75 g of Lactobacillus plantarum is used instead of 25 g of Neurospora robusta and 50 g of Lactobacillus plantarum in step A2.

[0072] Example 1

[0073] A composition containing probiotics, comprising the following raw materials by weight:

[0074] 20 parts of compound probiotics, 28 parts of plant active ingredients, 15 parts of soy fiber, 3.5 parts of erythritol;

[0075] The plant active ingredients include, by weight, 20 parts of baicalin nanoparticles A and 8 parts of eucommia extract A;

[0076] The composite probiotic comprises, by weight, 4.8 parts of Bacillus subtilis, 4.5 parts of Lactobacillus rhamnosus, 4.5 parts of Lactobacillus acidophilus and 6.2 parts of Bifidobacterium longum.

[0077] The preparation method of the composition containing probiotics in this embodiment comprises the following steps:

[0078] The composite probiotics, plant active ingredients, soybean fiber and erythritol were mixed and stirred for 3 hours to obtain a composition containing probiotics.

[0079] Example 2

[0080] A composition containing probiotics, comprising the following raw materials by weight:

[0081] 15 parts of compound probiotics, 20 parts of plant active ingredients, 10 parts of oat fiber, 2 parts of maltitol;

[0082] The plant active ingredients include, by weight, 15 parts of baicalin nanoparticles A and 5 parts of Eucommia ulmoides extract A;

[0083] The composite probiotic comprises, by weight, 3.6 parts of Bacillus subtilis, 3.4 parts of Lactobacillus rhamnosus, 3.4 parts of Lactobacillus acidophilus and 4.6 parts of Bifidobacterium longum.

[0084] The preparation method of the composition containing probiotics in this embodiment comprises the following steps:

[0085] The composite probiotics, plant active ingredients, oat fiber and maltitol were mixed and stirred for 3 hours to obtain a composition containing probiotics.

[0086] Example 3

[0087] A composition containing probiotics, comprising the following raw materials by weight:

[0088] 25 parts of compound probiotics, 35 parts of plant active ingredients, 20 parts of inulin, 5 parts of isomalt;

[0089] The plant active ingredients include, by weight, 25 parts of baicalin nanoparticles A and 10 parts of eucommia extract A;

[0090] The composite probiotic comprises, by weight, 6 parts of Bacillus subtilis, 5.5 parts of Lactobacillus rhamnosus, 5.5 parts of Lactobacillus acidophilus and 8 parts of Bifidobacterium longum.

[0091] The preparation method of the composition containing probiotics in this embodiment comprises the following steps:

[0092] The composite probiotics, plant active ingredients, inulin and isomalt were mixed and stirred for 3 hours to obtain a composition containing probiotics.

[0093] Example 4

[0094] A composition containing probiotics and a preparation method thereof. The specific implementation manner is the same as that of Example 1, except that an equal amount of baicalin nanoparticles B is used to replace baicalin nanoparticles A.

[0095] Example 5

[0096] A composition containing probiotics and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of baicalin nanoparticles C is used to replace baicalin nanoparticles A.

[0097] Example 6

[0098] A composition containing probiotics and a preparation method thereof. The specific implementation manner is the same as that of Example 1, except that an equal amount of Eucommia ulmoides extract B is used to replace Eucommia ulmoides extract A.

[0099] Comparative Example 1

[0100] A composition containing probiotics and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of baicalin is used to replace baicalin nanoparticles A.

[0101] Comparative Example 2

[0102] A composition containing probiotics and a preparation method thereof. The specific implementation manner is the same as that of Example 1, except that an equal amount of Eucommia ulmoides is used to replace Eucommia ulmoides extract A.

[0103] Effect evaluation:

[0104] The compositions containing probiotics prepared in the above Examples 1-6 and Comparative Examples 1-2 were tested, and the specific results are shown in Table 1.

[0105] Performance Test:

[0106] (1) Establishment of rat obesity model: 100 male SD adult rats were randomly divided into 10 groups, with free access to food and water, and their body weight ranged from 250 to 280 g. They were housed in single cages and fed with ordinary feed for 3 days during the adaptation period. After the end of the period, 10 rats in the blank control group continued to be fed with ordinary feed, and the remaining 90 rats were given high-calorie feed as the model group. Each group was fed with the same amount of food. After 2 weeks of continuous feeding, the rat obesity model was established; wherein, the high-calorie model feed was prepared according to the requirements of Appendix 8 (weight loss function evaluation method) in the National Food and Drug Administration

[2012] No. 107;

[0107] (2) Administration of the test substance: In the obesity model group randomly divided into 9 groups, the probiotic composition prepared in the embodiment and the comparative example was added to the high-calorie feed, and the addition amount was 3wt%. The model control group was fed only with the high-calorie model feed. The test substance was administered for 6 weeks. After the last administration of the sample, the rats were weighed after fasting for 16 hours. The rats were anesthetized with 1% sodium pentobarbital [0.5mL / (100g bw)], and the perinephric fat and perinephric fat were dissected and weighed. The fat / body ratio was calculated, and the average initial weight and average final weight of the rats were counted.

[0108] Table 1

[0109] Serial number Average initial weight (g) Average final weight (g) Fat / body ratio (%) Example 1 375.6 498.7 2.65 Example 2 377.6 504.1 2.71 Example 3 376.9 500.4 2.69 Example 4 377.2 528.4 2.95 Example 5 374.8 532.8 3.04 Example 6 377.0 517.6 2.84 Comparative Example 1 375.4 538.7 3.15 Comparative Example 2 375.5 530.3 2.98 Model control group 376.4 548.6 3.89 Blank control group 345.2 461.5 2.56

[0110] From the results in Table 1, it can be seen that the compositions containing probiotics prepared in Examples 1-3 have good weight loss effects and can effectively reduce the fat / body ratio.

[0111] Compared with Example 1, when preparing baicalin nanoparticles, Example 4-5 changed the total amount of papain and acid protease, and the enzymatic hydrolysis process failed to achieve efficient conversion; Example 5 changed the mass ratio of baicalin in baicalin alkali solution and corn protein powder in corn oligopeptide, and the loading effect was poor; at the same time, compared with Example 1, Comparative Example 1 replaced baicalin nanoparticles A with an equal amount of commercially available baicalin. All of the above will further reduce the bioavailability of baicalin and affect the weight loss effect.

[0112] Compared with Example 1, Example 6 changes the type of bacteria when preparing Eucommia ulmoides extract; meanwhile, compared with Example 1, Comparative Example 2 replaces Eucommia ulmoides extract A with an equal amount of commercially available Eucommia ulmoides, which reduces the content of the extracted diuretic and laxative components, affects the body's metabolism, and further affects the weight loss effect.

[0113] The above is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present application. Although the present application is disclosed as above in the preferred embodiment, it is not intended to limit the present application. Any technician familiar with the profession, without departing from the scope of the technical solution of the present application, using the technical content disclosed above to make slight changes or modifications are equivalent to equivalent implementation cases. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution.

Claims

1. A composition comprising probiotics, characterized in that The invention comprises the following raw materials by weight: 15-25 parts of compound probiotics, 20-35 parts of plant active ingredients, 10-20 parts of dietary fiber, and 2-5 parts of flavoring agents; wherein the plant active ingredients are a combination of baicalin nanoparticles and eucommia extract.

2. A composition comprising probiotics according to claim 1, characterized in that: The composite probiotics are a composition of bacillus subtilis, lactobacillus rhamnosus, lactobacillus acidophilus and bifidobacterium longum.

3. A composition comprising probiotics according to claim 1, characterized in that: The plant active ingredients include the following raw materials by weight: 15-25 parts of baicalin nanoparticles and 5-10 parts of eucommia extract.

4. A composition comprising probiotics according to claim 1, characterized in that: The preparation method of baicalin nanoparticles comprises the following steps: S1. adding baicalin to an alkaline solution, heating and stirring, and cooling to form a baicalin alkaline solution; S2. Disperse corn gluten powder in water, stir, add papain, and hydrolyze for 2-3 hours at a temperature of 50-60°C and a pH of 5-7, then adjust the pH to 3-4 and the temperature to 40-45°C, add acidic protease for hydrolysis for 2-3 hours, inactivate the enzyme, and filter to obtain corn oligopeptide; S3. The baicalin alkali solution obtained in step S1 and the corn oligopeptide obtained in step S2 are mixed, the pH value is adjusted to 10-12, stirred for 2-3 hours, 10%-20% by mass fraction of glucose-δ-lactone aqueous solution is added to adjust the pH value to 5-6.5, freeze-dried, and ground to obtain baicalin nanoparticles.

5. A composition comprising probiotics according to claim 4, characterized in that: The total amount of papain and acid protease in step S2 is 1%-2% of the mass of corn gluten powder.

6. A composition comprising probiotics according to claim 4, characterized in that: The mass ratio of baicalin to corn oligopeptide in the baicalin alkali solution in step S3 is 1:(3-6).

7. A composition comprising probiotics according to claim 1, characterized in that: The preparation method of Eucommia ulmoides extract comprises the following steps: A1. Wash the Eucommia ulmoides, dry it, grind it into powder, stir it with water, add a complex enzyme to hydrolyze it, and obtain an enzymatic solution; A2. A 3wt%-6wt% aqueous solution of glucose was added to the enzymatic solution obtained in step A1, sterilized, added with composite bacteria for fermentation, sterilized, and obtained a fermentation product; A3. The fermentation product obtained in step A2 is centrifuged, filtered, dried, ultrasonically extracted 2-3 times with 50wt%-70wt% ethanol, centrifuged, filtered, membrane concentrated, and spray-dried to obtain an eucommia extract.

8. A composition comprising probiotics according to claim 7, characterized in that: The composite bacteria are Neurospora robusta and Lactobacillus plantarum, and the mass ratio of the two is 1:(1.5-2.5).

9. A method for preparing a composition comprising probiotics according to any one of claims 1 to 8, characterized in that: The following steps are included: The composite probiotics, plant active ingredients, dietary fiber and flavoring agents are mixed and stirred to obtain a composition containing probiotics.

10. Use of the composition comprising probiotics according to any one of claims 1 to 8 in weight loss, characterized in that: The dosage form of the composition is granules, powders, tablets or capsules.