Lipopeptide composition for controlling plant diseases and insect pests

Abstract

The invention provides a lipopeptide composition for preventing and treating plant diseases and insect pests and application of the lipopeptide composition. The lipopeptide composition disclosed by the invention can be used for remarkably inhibiting the growth of various common plant pathogenic bacteria and nematodes, and has good antibacterial and insecticidal effects. And compared with a control group, after being treated by the composition, the plant growth is promoted, the number of plant products is larger, and / or the yield is higher. The lipopeptide composition provided by the invention has good biodegradability, and is pollution-free, green and environment-friendly. The lipopeptide composition provided by the invention has the advantages of definite components, less influence by environmental conditions, stable action effect, simple preparation and use methods and convenience in management.

Description

Technical Field

[0001] The present application relates to the field of biological control technology, and specifically to a lipopeptide composition and its use. Background Art

[0002] The emergence and outbreak of agricultural pests and diseases will not only affect the yield of crops, but also reduce the quality and market value of crops, further hindering the sustainable development of the agricultural sector. Pests may show characteristics such as periodicity, continuity, and uncertainty due to differences in natural factors such as region, climate, and insect species. If management and response are not timely, it is easy to cause large-scale crop damage. How to achieve effective prevention and control of crop pests is a key issue that needs to be urgently addressed in agricultural production. At the same time, pathogenic microorganisms in the soil are also an important cause of crop diseases. Factors such as excessive development of soil resources and unscientific and rational use of fertilizers and pesticides have caused soil to become the main transmission medium for a variety of crop pathogens. Among them, fungal microorganisms represented by mold have caused more than 70% of crop diseases. Under conditions that are conducive to the spread of rice blast fungi, rice blast can cause a reduction of up to 50% in rice yield, seriously restricting rice production worldwide. In addition to major diseases of cereal crops such as rice blast, wheat head blight, and corn ear rot, solanaceous wilt caused by Fusarium oxysporum and root rot of solanaceous and melons caused by Fusarium solani have also become a major obstacle to high-quality and efficient production of crops. Taking potatoes, which are widely planted around the world, as an example, potato wilt caused by Fusarium fungi can cause nearly 30-50% yield loss and quality decline, and has now become one of the most serious diseases in the world's crop industry.

[0003] The prevention and control of crop diseases and insect pests actively responds to the policy of "prevention first, integrated prevention and control". At present, it mainly includes three categories: chemical control, biological control and mechanical control. Mechanical control is to interfere with the growth and development of harmful organisms by physical factors or mechanical action. The actual operation is relatively complicated and is mostly used for prevention or emergency remediation. The relatively common chemical control mainly works through pesticides. Long-term use or improper use can easily cause problems such as pesticide residues, environmental pollution, and drug resistance, which in turn threaten human health. Compared with traditional pesticides, biological control has received widespread attention in the field of crop disease and insect pest control due to its environmental friendliness and sustainability. Among them, the rational use of microorganisms plays an important role in achieving plant disease control. For example, Pseudomonas, Bacillus, Trichoderma, etc. can effectively prevent soil-borne diseases of crops caused by pathogens, and Bacillus thuringiensis, Beauveria bassiana, etc. can be used as low-toxic insecticides for pest control. However, most microorganisms that can be used for biological control have complex metabolite spectra, the types of effective compounds are not clear, and the control effect is often unstable due to restrictions on host species, pathogen types, environmental conditions and other factors. Therefore, how to effectively control plant diseases and insect pests based on abundant microbial resources and related metabolites has become a difficult and hot topic in agricultural research.

[0004] Some studies have shown that the secondary metabolites of biocontrol microorganisms are the key to their control. Among them, lipopeptides are a class of low molecular weight biosurfactants that contain both hydrophilic amino acids and hydrophobic fatty acid chains, mainly produced by fermentation of microorganisms such as Bacillus, Pseudomonas, and Streptomyces. Lipopeptide compounds have diverse structures and functions. Due to different carbon chain lengths and amino acid compositions, they are divided into different families and have different functions. In addition to application fields such as petrochemicals, food industry, and environmental protection, lipopeptides have shown great application potential in the fields of medicine and agriculture due to their broad-spectrum antibacterial activity. Studies have shown that lipopeptide compounds have excellent effects in antibacterial and insecticidal effects, and are suitable for development as new environmentally friendly agents for the control of crop diseases and pests. However, a single type of lipopeptide has certain limitations in the application process, such as limited effect on inhibiting certain specific pathogens or being easily affected by environmental factors, resulting in unsatisfactory effects. Therefore, how to compound single lipopeptide compounds to achieve broad-spectrum control of plant diseases and insect pests is one of the important directions for high-quality development in the field of biological control. Summary of the invention

[0005] In view of the problems existing in the prior art, the present application provides a lipopeptide composition and its use.

[0006] 1. A lipopeptide composition, comprising two or three of a first lipopeptide, a second lipopeptide and a third lipopeptide, wherein the first lipopeptide comprises a peptide chain consisting of 1-7 amino acid residues, the second lipopeptide comprises a peptide chain consisting of 8-12 amino acid residues, and the third lipopeptide comprises a peptide chain consisting of 13 or more amino acid residues.

[0007] 2. The lipopeptide composition according to item 1, wherein the first lipopeptide is selected from one or more of surfactin, iturin, lichenin, mycosubtilin, fusarin, bacillus antimycin, bacitracin, babylonin, pamilasin, kurstak, agrobactin, and cyclobactin;

[0008] The second lipopeptide is selected from one or more of fenmucin, phospholipid inhibitor, polymyxin, daptomycin, octapeptidin, and bacillus subtilisin;

[0009] The third lipopeptide is selected from one or more of polypeptide bacteriocin and tridecapeptidin.

[0010] 3. The lipopeptide composition according to item 1 or 2, wherein the lipopeptide composition comprises two of the first lipopeptide, the second lipopeptide and the third lipopeptide, and the mass ratio of any two lipopeptides in the composition is 1:(0.02-50), preferably, the mass ratio of any two lipopeptides in the composition is 1:(0.1-10).

[0011] 4. The lipopeptide composition according to item 1 or 2, wherein the lipopeptide composition comprises three of a first lipopeptide, a second lipopeptide and a third lipopeptide, and the mass ratio of any three lipopeptides in the composition is 1:(0.02-50):(0.02-50), preferably, the mass ratio of any three lipopeptides in the composition is 1:(0.1-10):(0.1-10).

[0012] 5. The lipopeptide composition according to any one of items 1 to 4, wherein the concentration of each lipopeptide in the composition is 0.0001-300 g / L.

[0013] 6. Use of the lipopeptide composition described in any one of items 1 to 5 in preventing and controlling crop diseases or insect pests.

[0014] 7. The use according to item 6, wherein the disease is a disease caused by bacteria or a disease caused by fungi,

[0015] Preferably, the bacterium is selected from one of the genus Pseudomonas, the genus Xanthomonas, the genus Erwinia and the genus Ralstonia.

[0016] Preferably, the fungus is selected from one of the following: Pythium, Phytophthora, Achlya, Pasasitica, Phytophthora, Rhizopus, Penicillium, Aspergillus, Botrytis, Botryosphaeria, Fusarium, Sclerotinia, Rhizoctonia, Colletotrichum, Alterna ria), Colletotrichum, Spongospora, Venturia, Valsa, Piriformospora, Ustilagonoidea, Helminthosporium, Puccinia, Physoderma, Cercospora, Curvularia, Ustilago, Erysiphe, Gladaxporism, Septoria.

[0017] 8. The use according to item 7, wherein the pest is a nematode pest or an insect pest,

[0018] Preferably, the nematode is selected from one of the following: root-knot nematodes, cyst nematodes, root-rot nematodes, root-knot nematodes, stem nematodes, semi-piercing nematodes, kidney-shaped nematodes, leaf nematodes, long-needle nematodes, Xiphinema,

[0019] Preferably, the insect is selected from one of the following: Coleopteran pests, Lepidopteran pests, Hemiptera pests, Diptera pests, Isoptera pests, Blattodea pests.

[0020] 9. Use of the lipopeptide composition described in any one of items 1 to 5 as an agricultural antibacterial agent, agricultural insecticide, agricultural adjuvant, agricultural fertilizer or pesticide.

[0021] Effects of the Invention

[0022] This application has the following technical advantages:

[0023] (1) The lipopeptide composition of the present application can significantly inhibit the growth of a variety of common plant pathogens and pests, and has good antibacterial and insecticidal effects. Moreover, compared with the control group, after being treated with the composition, the growth of plants is significantly promoted, and the number of plant products is greater and / or the yield is higher.

[0024] (2) The lipopeptide composition of the present application has good biodegradability, is pollution-free, and is green and environmentally friendly.

[0025] (3) The lipopeptide composition of the present application has clear ingredients, is less affected by environmental conditions, has stable effects, is simple to prepare and use, and is easy to manage. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a diagram showing the antibacterial effect of the lipopeptide composition on common plant pathogens.

[0027] Figure 2 This is a diagram showing the contact killing effect of the lipopeptide composition on the second-instar larvae of the southern root-knot nematode.

[0028] Figure 3 Shown is a diagram showing the effect of spraying the lipopeptide composition on promoting the growth of cucumber plants.

[0029] Figure 4 Shown is a diagram showing the effect of spraying a lipopeptide composition on promoting the growth of highland barley plants.

[0030] Figure 5 The figure shows the effect of lipopeptide composition irrigation treatment on preventing and controlling cereal plant diseases and increasing yield.

[0031] Figure 6 The figure shows the effect of the lipopeptide composition watering treatment to effectively prevent and control pumpkin aphid diseases and increase yield. DETAILED DESCRIPTION

[0032] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0033] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0034] In response to the problems existing in the prior art, the present application provides a lipopeptide composition, comprising two or three of a first lipopeptide, a second lipopeptide and a third lipopeptide, wherein the first lipopeptide comprises a peptide chain consisting of 1-7 amino acid residues, the second lipopeptide comprises a peptide chain consisting of 8-12 amino acid residues, and the third lipopeptide comprises a peptide chain consisting of 13 or more amino acid residues.

[0035] In a specific embodiment, the first lipopeptide is selected from one or more of surfactin, iturin, lichenin, mycosubtilin, fusarin, bacillus antimycin, bacitracin, babylonin, pamilasin, kurstak, agrobactin, and cyclobactin.

[0036] In a specific embodiment, the second lipopeptide is selected from one or more of fenmucin, phosphatidylinositol, polymyxin, daptomycin, octapeptidin, and bacillus.

[0037] In a specific embodiment, the third lipopeptide is selected from one or more of polypeptide bacteriocin and tridecapeptidin.

[0038] In a specific embodiment, the lipopeptide composition comprises a first lipopeptide and a second lipopeptide.

[0039] In a specific embodiment, the lipopeptide composition comprises a first lipopeptide and a third lipopeptide.

[0040] In a specific embodiment, the lipopeptide composition comprises a second lipopeptide and a third lipopeptide.

[0041] In a specific embodiment, the lipopeptide composition includes a first lipopeptide and a second lipopeptide, and the mass ratio of the first lipopeptide to the second lipopeptide is 1:(0.02-50), for example, it can be 1:0.02, 1:0.05, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:50, and any values ​​between these values.

[0042] In a specific embodiment, the mass ratio of the first lipopeptide to the second lipopeptide is 1:(0.1-10).

[0043] In a specific embodiment, the lipopeptide composition comprises a first lipopeptide and a third lipopeptide, and the mass ratio of the first lipopeptide to the third lipopeptide is 1:(0.02-50), for example, 1:0.02, 1:0.05, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1 :10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:50, and any values ​​in between.

[0044] In a specific embodiment, the mass ratio of the first lipopeptide to the third lipopeptide is 1:(0.1-10).

[0045] In a specific embodiment, the lipopeptide composition comprises a second lipopeptide and a third lipopeptide, and the mass ratio of the first lipopeptide to the third lipopeptide is 1:(0.02-50), for example, 1:0.02,

[0046] 1:0.05, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:50, and any number in between.

[0047] In a specific embodiment, the mass ratio of the second lipopeptide to the third lipopeptide is 1:(0.1-10).

[0048] In a specific embodiment, the lipopeptide composition includes fusaricide and octapeptidin, and the mass ratio of fusaricide to octapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0049] In a specific embodiment, the lipopeptide composition includes fusaricide and daptomycin, and the mass ratio of fusaricide to daptomycin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0050] In a specific embodiment, the lipopeptide composition includes fusaricide and polymyxin, and the mass ratio of fusaricide to polymyxin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0051] In a specific embodiment, the lipopeptide composition includes fusaricide and fenmucin, and the mass ratio of fusaricide to fenmucin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0053] In a specific embodiment, the lipopeptide composition includes surfactin and octapeptidin, and the mass ratio of surfactin to octapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0054] In a specific embodiment, the lipopeptide composition includes surfactin and daptomycin, and the mass ratio of surfactin to daptomycin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0055] In a specific embodiment, the lipopeptide composition includes surfactin and polymyxin, and the mass ratio of surfactin to polymyxin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0056] In a specific embodiment, the lipopeptide composition includes surfactin and fenmucin, and the mass ratio of surfactin to fenmucin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0058] In a specific embodiment, the lipopeptide composition includes lichenin and octapeptidin, and the mass ratio of lichenin to octapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0060] In a specific embodiment, the lipopeptide composition includes lichenin and daptomycin, and the mass ratio of lichenin to daptomycin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0062] In a specific embodiment, the lipopeptide composition includes lichenin and polymyxin, and the mass ratio of lichenin to polymyxin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0064] In a specific embodiment, the lipopeptide composition includes lichenin and fenmucin, and the mass ratio of lichenin to fenmucin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0065] In a specific embodiment, the lipopeptide composition comprises mycosubtiline and octapeptidin, and the mass ratio of mycosubtiline to octapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0066] In a specific embodiment, the lipopeptide composition comprises mycosubtiline and daptomycin, and the mass ratio of mycosubtiline to daptomycin is 1:(0.1-10), and can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0067] In a specific embodiment, the lipopeptide composition comprises mycosubtiline and polymyxin, and the mass ratio of mycosubtiline to polymyxin is 1:(0.1-10), and can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0068] In a specific embodiment, the lipopeptide composition comprises mycosubtiline and fenmucin, and the mass ratio of mycosubtiline to fenmucin is 1:(0.1-10), and can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0069] In a specific embodiment, the lipopeptide composition includes iturin and octapeptidin, and the mass ratio of iturin to octapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0070] In a specific embodiment, the lipopeptide composition includes iturin and daptomycin, and the mass ratio of iturin to daptomycin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0071] In a specific embodiment, the lipopeptide composition includes iturin and polymyxin, and the mass ratio of iturin to polymyxin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0072] In a specific embodiment, the lipopeptide composition includes iturin and fenmucin, and the mass ratio of iturin to fenmucin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0074] In a specific embodiment, the lipopeptide composition includes fusaricide and polypeptide bacteriocin, and the mass ratio of fusaricide to polypeptide bacteriocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0075] In a specific embodiment, the lipopeptide composition includes fusaricide and tridecapeptidin, and the mass ratio of fusaricide to tridecapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0076] In a specific embodiment, the lipopeptide composition includes surfactin and polypeptide bacteriocin, and the mass ratio of surfactin to polypeptide bacteriocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0077] In a specific embodiment, the lipopeptide composition includes surfactin and tridecapeptidin, and the mass ratio of surfactin to tridecapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0078] In a specific embodiment, the lipopeptide composition includes lichenin and polypeptide streptocin, and the mass ratio of lichenin to polypeptide streptocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0080] In a specific embodiment, the lipopeptide composition includes lichenin and tridecapeptidin, and the mass ratio of lichenin to tridecapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0081] In a specific embodiment, the lipopeptide composition comprises mycosubtiline and polypeptide streptocin, and the mass ratio of mycosubtiline to polypeptide streptocin is 1:(0.1-10), and can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0082] In a specific embodiment, the lipopeptide composition includes mycosubtilin and tridecapeptidin, and the mass ratio of lichenin and peptide streptocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0083] In a specific embodiment, the lipopeptide composition includes iturin and polypeptide streptocin, and the mass ratio of iturin and polypeptide streptocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0084] In a specific embodiment, the lipopeptide composition includes iturin and tridecapeptidin, and the mass ratio of iturin to tridecapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0085] In a specific embodiment, the lipopeptide composition includes octapeptidin and polypeptide bacteriocin, and the mass ratio of octapeptidin to polypeptide bacteriocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0087] In a specific embodiment, the lipopeptide composition includes octapeptide and tridecapeptide, and the mass ratio of iturin and polypeptide is 1: (0.1-10), which can be, for example, 1: 0.2, 1: 0.3, 1: 0.4, 1: 0.5, 1: 0.6, 1: 0.7, 1: 0.8, 1: 0.9, 1: 1, 1: 1.5, 1: 2, 1: 2.5, 1: 3, 1: 3.5, 1: 4, 1: 4.5, 1: 5, 1: 5.5, 1: 6, 1: 6.5, 1: 7, 1: 7.5, 1: 8, 1: 8.5, 1: 9, 1: 9.5.

[0088] In a specific embodiment, the lipopeptide composition includes daptomycin and polypeptide bacteriocin, and the mass ratio of daptomycin to polypeptide bacteriocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0090] In a specific embodiment, the lipopeptide composition includes daptomycin and tridecapeptidin, and the mass ratio of iturin and polypeptide is 1: (0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0091] In a specific embodiment, the lipopeptide composition includes polymyxin and polypeptide bacteriocin, and the mass ratio of polymyxin to polypeptide bacteriocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0093] In a specific embodiment, the lipopeptide composition includes polymyxin and tridecapeptidin, and the mass ratio of iturin and polypeptide is 1: (0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0094] In a specific embodiment, the lipopeptide composition includes mustard and polypeptide streptocin, and the mass ratio of mustard to polypeptide streptocin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0095] In a specific embodiment, the lipopeptide composition includes fenmucin and tridecapeptidin, and the mass ratio of fenmucin to tridecapeptidin is 1:(0.1-10), which can be, for example, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5.

[0096] In a specific embodiment, the lipopeptide composition comprises a first lipopeptide, a second lipopeptide and a third lipopeptide. In a specific embodiment, the mass ratio of any three lipopeptides in the composition is 1:(0.02-50):(0.02-50). That is, the mass ratio of the first lipopeptide, the second lipopeptide, and the third lipopeptide can be 1:(0.02-50):(0.02-50), or the mass ratio of the first lipopeptide, the third lipopeptide, and the second lipopeptide can be 1:(0.02-50):(0.02-50), or the mass ratio of the second lipopeptide, the first lipopeptide, and the third lipopeptide can be 1:(0.02-50):(0.02-50), or the mass ratio of the second lipopeptide, the third lipopeptide, and the first lipopeptide can be 1:(0.02-50):(0.02-50), or the mass ratio of the third lipopeptide, the first lipopeptide, and the second lipopeptide can be 1:(0.02-50):(0.02-50), or the mass ratio of the third lipopeptide, the second lipopeptide, and the first lipopeptide can be 1:(0.02-50):(0.02-50).

[0097] In the lipopeptide composition of the present application, the content of each lipopeptide (if present) is 0.0001-300 g / L, for example, it can be 0.0001 g / L, 0.001 g / L, 0.01 g / L, 0.1 g / L, 0.5 g / L, 1 g / L, 2 g / L, 5 g / L, 10 g / L, 20 g / L, 30 g / L, 40 g / L, 50 g / L, 60 g / L, 70 g / L, 80 g / L, 90 g / L, 100 g / L, 200 g / L, 300 g / L, and any value between these values. For example, if the lipopeptide composition includes a first lipopeptide and a second lipopeptide, the content of the first lipopeptide is 0.0001-300 g / L, and the content of the second lipopeptide is 0.0001-300 g / L.

[0098] The lipopeptide composition of the present application can be formulated into various dosage forms according to actual needs, such as powder, liquid, suspension, wet agent, aerosol, granule, etc.

[0099] The lipopeptide composition of the present application is applied by the following methods: seed coating, seed dressing and soaking, soil treatment, spraying, watering, wiping, fumigation, film covering, and injection drilling.

[0100] The lipopeptide composition of the present application can not only effectively inhibit the growth of nematodes, insects and plant pathogens, but also has a broad-spectrum antibacterial effect, with an antibacterial rate of up to 100%, and can be used to prevent and control agricultural diseases caused by plant pests or pathogens. Furthermore, the lipopeptide composition can promote plant growth while preventing and controlling plant diseases and insect pests.

[0101] The present application also provides the use of any one of the above-mentioned lipopeptide compositions in preventing and controlling crop diseases and insect pests.

[0102] The pests and diseases may include diseases and pests known in the art.

[0103] In a specific embodiment, the pests and diseases are diseases caused by bacteria or fungi, such as spot disease, leaf blight, bacterial wilt, canker, rot and the like.

[0104] The bacterial pathogenic microorganisms may specifically be Pseudomonas bacteria, Xanthomonas bacteria, Erwinia bacteria, Ralstonia bacteria, etc. The Pseudomonas bacteria is preferably selected from the following group: Pseudomonas syringae, Pseudomonas savastanoi; the Xanthomonas bacteria is preferably selected from the following group: Xanthomonas campestris, Xanthomonas arboricola, Xanthomonas axonopodis; the Erwinia bacteria may specifically be Erwinia amylovory; the Ralstonia bacteria may specifically be Ralstonia solanacearum.

[0105] The fungus may be selected from the group consisting of Pythium, Phytophthora, Achlya, Pasasitica, Phytophthora, Rhizopus, Penicillium, Aspergillus, Botrytis, Botryosphaeria, Fusarium, Sclerotinia, Rhizoctonia, Colletotrichum, Alternaria ), Colletotrichum, Spongospora, Venturia, Valsa, Piriformospora, Ustilagonoidea, Helminthosporium, Puccinia, Physoderma, Cercospora, Curvularia, Ustilago, Erysiphe, Gladaxporism, Septoria, etc.

[0106] In a specific embodiment, the pest is a nematode pest or an insect pest.

[0107] Among them, the nematode is preferably selected from the following group: root-knot nematodes, cyst nematodes, root-rot nematodes, root-knot nematodes, stem nematodes, semi-puncturing nematodes, kidney-shaped nematodes, leaf nematodes, long needle nematodes, and Xiphinema.

[0108] The insects are preferably selected from the following groups: coleoptera pests, lepidoptera pests, hemiptera pests, diptera pests, isoptera pests, and blatata pests. The lepidoptera pests may specifically be one or more of carob moth, beet armyworm, fall armyworm, Mediterranean mealybug, cotton bollworm, diamondback moth, and rapae butterfly; the hemiptera pests may specifically be one or more of rose apple aphid, peach aphid, pea aphid, whitefly, and rice planthopper; the diptera pests may specifically be culex; the isoptera pests may specifically be one or more of yellow-winged macrotermes, black-winged earth termites, and yellow-chested termites; the blatata pests may specifically be one or more of Japanese cockroaches, American cockroaches, black-chested cockroaches, and Australian cockroaches.

[0109] The present application also provides the use of any one of the above-mentioned lipopeptide compositions as an agricultural antibacterial agent, agricultural insecticide, agricultural adjuvant, agricultural fertilizer or pesticide.

[0110] Example

[0111] Surfactin, daptomycin, fenmucin, and iturin were purchased from MedChemExpress, octapeptidin, licheniformis, fusarium, peptide mycin, and tridecapeptidin were purchased from Zhejiang Hunda Biotechnology Co., Ltd., polymyxin was purchased from Sigma, and mycosubtilin was purchased from Hangzhou Peptidejia Biotechnology Co., Ltd.

[0112] Example 1 Obtaining a lipopeptide composition

[0113] Class I lipopeptides (fusaricide, surfactin, licheniformis, mycobacterium, iturin), Class II lipopeptides (octapeptidin, daptomycin, polymyxin, fenmucin) and Class III lipopeptides (polypeptidin, tridecapeptidin) were dissolved in water and prepared into 1g / L aqueous solutions, which were respectively used as compositions 1-11 of the present application. Two of the Class I lipopeptides, Class II lipopeptides and Class III lipopeptides were prepared into aqueous solutions at a mass ratio of 1:1 as compositions 12-49 of the present application, and the concentration of each type of lipopeptide in the composition was 0.5g / L; the types and mass ratios of lipopeptides involved in the preparation of compositions 50-64 are shown in Table 1, and the concentration of Class I lipopeptides was controlled to be 0.5g / L during the preparation process.

[0114] Table 1

[0115]

[0116]

[0117]

[0118] Example 2 Evaluation of the Indoor Antibacterial Effect of Lipopeptide Compositions on Common Plant Pathogenic Bacteria

[0119] Activation of pathogenic bacteria. Different pathogenic bacteria required for the experiment were inoculated on LB medium for activation and placed in a 37°C incubator for culture until the colonies grew to 2 / 3 of the medium.

[0120] Plate confrontation antibacterial experiment of different pathogenic bacteria. The plate confrontation method was used to detect the inhibitory effect of the lipopeptide composition 1-64 on the growth of pathogenic bacteria. Under aseptic operation conditions, the pre-melted sterilized culture medium was quantitatively added to a sterile conical flask, and the lipopeptide composition 1-64 in Example 1 was quantitatively aspirated and added to the conical flask respectively. After fully shaking, an equal amount of plate culture medium containing the lipopeptide composition was prepared (specifically, 24 mL of culture medium was taken, 1% of the lipopeptide composition was added and fully shaken, and 3 culture dishes with a diameter of 6 cm were taken and poured with an equal amount of about 8 mL of culture medium to prepare a plate culture medium containing the corresponding lipopeptide composition). The experiment set up a clean water treatment group as the control group, and each group was treated with no less than 3 replicates.

[0121] After the center of the plate was determined by the cross method, a sterile puncher with a diameter of 5 mm was used to punch holes at the edge of the activated pathogenic bacterial colony and take the bacterial cake. The side with mycelium on the bacterial cake was transferred to the center of the above LB plate with a sterile toothpick facing down and placed in a 37°C incubator for culture. After the culture was completed, according to the growth of pathogens in the blank control culture dish, the diameter, antibacterial bandwidth and other indicators of each pathogen in the different lipopeptide composition treatment groups were observed and measured by the cross method, and the antibacterial rate was calculated by the following formula. The results verified the inhibitory effect of different lipopeptide compositions on the growth of pathogens.

[0122] Bacterial inhibition rate (%) = [(pathogen diameter of control group - diameter of bacterial cake) - (pathogen diameter of experimental group - diameter of bacterial cake)] / (pathogen diameter of control group - diameter of bacterial cake) × 100%. The experimental results are shown in Table 2.

[0123] Table 2

[0124]

[0125]

[0126]

[0127] Example 3 Evaluation of the Indoor Antibacterial Effect of Lipopeptide Compositions on Common Plant Pathogenic Fungi

[0128] Activation of pathogenic fungi. Based on the different pathogenic fungi required for the experiment, they were inoculated on PDA culture medium for activation and placed in a 25°C, 12h light, 12h dark incubator for culture until the colony grew to 2 / 3 of the culture medium. The plate confrontation antibacterial experiment was the same as in Example 2, wherein the transfer plate involved was a PDA plate, and was placed in a 25°C, 12h light, 12h dark incubator for culture. The antibacterial rate calculation formula is the same as in Example 2. The experimental results are shown in Table 3.

[0129] Table 3

[0130]

[0131]

[0132]

[0133]

[0134] It has been determined that the surfactant, lichenin, iturin, mycobacterium, fusarium, fenmustine, polymyxin, daptomycin, octapeptide, peptide and tridecapeptide in the present application all have broad-spectrum antagonism, and have good growth inhibition effects on the above-mentioned common plant pathogenic fungi. The inhibition rate against watermelon sclerotinia, powdery mildew, tomato gray mold and pepper anthracnose can reach 100% (see Figure 1 ). Among them, the antibacterial rates of different lipopeptide compound compositions were higher than those of the single lipopeptide compound treatment group under the same conditions.

[0135] Example 4 Evaluation of the contact killing effect of lipopeptide composition on insects

[0136] The insect contact killing experiment adopts the micro-drip method. Place a medium-sized, fresh rapeseed leaf in a culture dish containing a 9-cm diameter filter paper, select 20 3rd-instar larvae of the test insect diamondback moth with basically the same growth condition and without any treatment and place them on the leaf, use a micro-syringe to extract 1 μL of the prepared lipopeptide composition solution, and drip it on the pronotum of the test insect. Similarly, place a medium-sized, fresh watermelon leaf in a culture dish containing a 9-cm diameter filter paper, select 20 3rd-instar nymphs of the test insect whitefly with basically the same growth condition and without any treatment and place them on the leaf, use a micro-syringe to extract 1 μL of the prepared lipopeptide composition solution, and drip it on the pronotum of the test insect. After the injection treatment, they were placed in a greenhouse for breeding, and the blank control was the clean water treatment group. Each treatment group was repeated 3 times. After 48 hours, the number of insect deaths in each treatment group was recorded, and the mortality rate and corrected mortality rate were calculated. The relevant calculation formula is as follows:

[0137] Mortality rate (%) = (number of test insect deaths / total number of test insects) × 100; Corrected mortality rate (%) = [(mortality rate of test insects in the treatment group - mortality rate of test insects in the control group) / (100 - mortality rate of test insects in the control group)] × 100. The experimental results are shown in Table 4.

[0138] Table 4

[0139]

[0140]

[0141]

[0142]

[0143] After different lipopeptide compounds were compounded, the growth inhibition effect on both insects was improved. The mortality rate after treatment with the two lipopeptide compositions 12-49 and the three lipopeptide compositions 50-64 was higher than that after treatment with the single lipopeptide composition 1-11 under the same conditions.

[0144] Example 5 Evaluation of the contact killing effect of the lipopeptide composition on southern root-knot nematodes

[0145] The second-instar larvae of southern root-knot nematodes were used as the test object, and the immersion method was used for determination, with 3 replicates for each treatment group. Take a 24-well cell culture plate, drop 10 μL of nematode suspension into each well, add 500 μL of composition 1-64, repeat 3 times, put it in a 28°C incubator, add an equal amount of clean water to the blank treatment, observe the second-instar larvae of root-knot nematodes under a microscope after 24 hours and 96 hours, judge the number of deaths and hatched eggs according to the morphology of the nematodes, and calculate the corrected mortality rate.

[0146] The calculation formula is as follows:

[0147] Mortality rate (%) = (number of 2nd instar larvae dead / total number of nematodes tested) × 100; Corrected mortality rate (%) = [(2nd instar larvae mortality rate in treatment group - 2nd instar larvae mortality rate in control group) / (100 - 2nd instar larvae mortality rate in control group)] × 100. The experimental results are shown in Table 5.

[0148] Table 5

[0149]

[0150]

[0151]

[0152]

[0153] After the different lipopeptide compounds were compounded, the growth inhibition effect on nematodes was improved. The mortality rate of the second-instar larvae after treatment with the two lipopeptide compositions 12-49 and the three lipopeptide compositions 50-64 was higher than that of the single lipopeptide composition 1-11 treatment group under the same conditions. At 96 hours, after treatment with the corresponding lipopeptide composition, the mortality rate of the second-instar larvae of the root-knot nematode was able to reach 100% (see Figure 2 ).

[0154] Example 6 Indoor antibacterial activity of lipopeptide compositions in different proportions against common plant pathogens

[0155] Based on the results of Example 2, based on the typical two-lipopeptide compound compositions 14, 20, 21, 26, 32, 38, 39, the antibacterial activity of common plant pathogens was explored according to the compounding ratio. The antibacterial rate calculation method is the same as that of Example 2. The EC of each agent was calculated by the software. 50 values, and the theoretical EC of the composition 50 Value, toxicity ratio (SR) = theoretical EC 50 Value / Determination EC 50 If the toxicity ratio SR>1.5, it is a synergistic effect, SR<0.5 is an antagonistic effect, and 0.5≤SR≤1.5 is an additive effect. The experimental results are shown in Table 6.

[0156] Table 6

[0157]

[0158]

[0159] The experimental results show that when the compounding ratio of the two lipopeptides is controlled between 50:1 and 1:50, there is an additive or synergistic effect. The application of the composition can greatly improve the antibacterial effect and save time and effort. When the compounding ratio is maintained between 1:0.1 and 1:10, the composition has a synergistic effect, and the antibacterial activity against common plant pathogens can be further improved through compounding.

[0160] Example 7 Evaluation of lipopeptide compositions for the control of common plant diseases in the field

[0161] 1. Effects of seed soaking with lipopeptide composition on plant seedlings

[0162] Seed treatment. For plants such as highland barley (Gramineae), peanut (Leguminosae), rapeseed (Cruciferae), potato (Solanaceae), cucumber (Cucurbitaceae), spinach (Leguminosae), lettuce (Asteraceae), and cotton (Malvaceae), select full seeds of uniform size and soak them with compositions 2, 3, 5, 8, 9, 10, 21, 22, 26, 27, 38, 40, 53, and 57 at a ratio of seeds: treatment solution = 1:1.5 for 24 hours. The water treatment was used as a blank control, and each treatment was repeated 3 times.

[0163] After the seeds were sown and the seedlings emerged, the growth of each plant seedling was investigated and recorded every 5 days, including the number of dead seedlings and the total number of plants, until no more seedlings died, and the death rate was calculated. The experimental results are shown in Table 7.

[0164] Table 7

[0165]

[0166]

[0167] It was determined that compared with the blank control (11.72-15.67%), the seedling death rate after the seed was treated with the above composition was reduced, and the seedling emergence was better than that of the control group. Among them, the average seedling death rate of the plants in the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups was 10.27-14.51%, and the average seedling death rate of the plants in the lipopeptide compound composition 21, 22, 26, 27, 38, 40, 53, 57 treatment groups was 6.26-12.89%. For the composition 38 treatment group, the seedling death rate was reduced by 25.77-40.45% compared with the control group under different plant growth conditions. Although the single lipopeptide composition treatment can also significantly reduce the seedling death rate, the effect of the lipopeptide compound composition treatment group is significantly better than the single lipopeptide composition treatment group under the same period, and the plant seedling death situation is significantly improved.

[0168] 2. Effects of spraying lipopeptide combination on plant diseases during growth period

[0169] The above composition was taken and sprayed on plants (highland barley, peanuts, potatoes, cucumbers, and cotton) 60 days after the seeds of the above plants emerged, and the spraying was continued for 3 times with an interval of 10 days. The growth of the plants was randomly sampled and investigated 5 days after the last application of the pesticide, and 5 points were randomly sampled in each area to check the incidence of leaf spots, grade them, and calculate the disease index and relative control effect.

[0170] The grading standard is as follows: 0: normal leaves, no lesions; 1: a small number of lesions on the leaves (1-2); 2: a moderate number of lesions on the leaves (3-5); 3: a large number of lesions on the leaves (6-8); 4: more than 9 lesions on the leaves, the area of ​​lesions accounts for less than 50% of the total leaf area; 5: the area of ​​lesions accounts for more than 50% of the total leaf area. Among them, the disease index = 100 × ∑ (number of diseased plants at each level × level) / (total number of plants × highest level representative value), relative control effect (%) = (control group disease index - treatment group disease index) / control group disease index × 100. The experimental results are shown in Table 8.

[0171] Table 8

[0172]

[0173]

[0174] It was determined that continuous spraying of the lipopeptide combination can alleviate the disease situation of each plant. The average control effect of the treatment groups of composition 2, 3, 5, 8, 9, and 10 was 23.82-36.08%, and the average control effect of the treatment groups of lipopeptide compound composition 21, 22, 26, 27, 38, 40, 53, and 57 was 32.86-50.15%, respectively, which were significantly higher than the treatment groups of the single lipopeptide combination under the same conditions. The treatment of the lipopeptide combination can promote the growth of the plants while effectively preventing diseases, and the height of the plants has been significantly improved (see Figure 3 , Figure 4 ).

[0175] 3. Effect of lipopeptide composition irrigation treatment on leaf spot disease during plant growth period

[0176] Field test. Plant crops such as chestnut trees (Fagaceae), walnut trees (Juglandaceae), peach trees (Rosaceae), grapevines (Vitaceae), kiwifruit trees (Actinidiaceae), citrus trees (Rutaceae), and banana trees (Musaceae) were transplanted in the fields in different regions. Clean water treatment was used as a blank control, and the above-mentioned lipopeptide composition was irrigated. Each plant was irrigated 1500mL, once a month, and continued to be cultivated for 9 months after continuous irrigating for 3 months. The water and fertilizer management during the planting process was uniformly handled by the plantations in each region. Random sampling surveys were conducted at 5 points in each area to record the growth of the trees, check the incidence of lesions on the leaves of the trees, and classify them to calculate the disease index and relative control effect. The calculation method of the disease index and relative control effect is the same as that of plant growth period diseases. The experimental results are shown in Table 9.

[0177] Table 9

[0178]

[0179]

[0180] As for the plant crops involved in the experiment, the irrigation treatment of the lipopeptide combination can effectively slow down the incidence of plant crops and achieve the prevention and control of related diseases. The prevention and control effect varies depending on the type of plant crops. Among them, the prevention and control effect of the lipopeptide compound combination treatment group is significantly higher than that of the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups under the same conditions.

[0181] Example 8 Evaluation of the control of common plant nematode pests by lipopeptide compositions

[0182] 1. Prevention and treatment of tomato root-knot nematode disease by seed soaking with lipopeptide combination

[0183] The soaking treatment steps of tomato (Solanaceae) seeds were the same as those in Example 7, with water treatment as a blank control. The field was randomly divided into areas, each with 60m 2, sow each group of soaked seeds in 3 areas. 60 days after sowing, investigate the growth of tomatoes (plant height) and the incidence of root-knot nematodes. Randomly sample 5 points in each area, take 8 plants at each point, a total of 40 plants, dig them out with a shovel along with the soil of the plant roots, wash them with clean water, check the number of root knots at the roots of tomatoes, grade them, and calculate the disease index and prevention and control effect. At the same time, survey and statistics are conducted on the number of fruits and single fruit weight during the harvest period.

[0184] The grading standards are as follows: 0: normal root system, no visible root knots; 1: a small number of root knots on the root system, accounting for 1-20% of the whole root system; 2: the number of root knots on the root system is moderate, accounting for 21-40% of the whole root system; 3: the number of root knots on the root system is large, accounting for 61-80% of the whole root system; 4: there are many root knots on the root system, accounting for 61-80% of the whole root system; 5: the number of root knots on the root system is very large, forming root knot clumps, accounting for 81-100% of the whole root system. Among them, the disease index = 100 × ∑ (number of diseased plants at each level × level) / (total number of plants × highest level representative value), relative control effect (%) = (control group disease index - treatment group disease index) / control group disease index × 100. The experimental results are shown in Table 10.

[0185] Table 10

[0186]

[0187]

[0188] As shown in Table 10, lipopeptides have good control effects on tomato root-knot nematode diseases, and during the test, no phytotoxicity of the composition to tomatoes was found, and the plants grew well. Among them, the average control effect of the lipopeptide composite composition treatment group was significantly higher than that of the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups under the same conditions, and can more effectively control plant-related nematode diseases.

[0189] The seed soaking treatment of the lipopeptide composition not only prevents and controls nematode pests, but also further promotes the growth of tomato plants. According to the measurement, the average height of the control group was 0.93m, and the average plant height of the plants in the treatment groups of compositions 2, 3, 5, 8, 9, and 10 was 1.05-1.08m, an increase of 12.90-16.13% compared with the control group; the average plant height of the plants in each treatment group of the lipopeptide compound composition was 1.14-1.25m, an increase of 22.58-34.40% compared with the control group. The average single fruit weight of the control group was 235g, the average single fruit weight of the treatment groups of compositions 2, 3, 5, 8, 9, and 10 was 245-249g, and the average single fruit weight of each treatment group of the lipopeptide compound composition was 254-264g, and the single fruit weight of the composition treatment group was 1.08-1.12 times that of the control group. The average plant height and single fruit weight of the groups treated with lipopeptide compound compositions 21, 22, 26, 27, 38, 40, 53, and 57 were higher than those of the group treated with a single lipopeptide composition under the same conditions.

[0190] 2. Prevention and control of wheat cereal cyst nematode disease by seed soaking with lipopeptide combination

[0191] The soaking treatment steps of wheat (Gramineae) seeds were the same as those in Example 4, with water treatment as a blank control. The field was randomly divided into areas, each with 60m 2 , sow each group of seeds after soaking in 3 areas. 60 days after sowing, investigate the growth of wheat (plant height) and the incidence of cereal cyst nematodes. Randomly sample 5 points in each area, take 8 plants at each point, a total of 40 plants, dig them out with a shovel together with the soil of the plant roots, wash them with clean water, check the number of cysts on the roots of wheat, grade them, and calculate the disease index and control effect. At the same time, survey and statistics are conducted on indicators such as yield and thousand-grain weight during the harvest period.

[0192] Grading standard: Grade 0: normal root system, no visible cysts; Grade 1: a small number of cysts on the root system (1-5 cysts / plant); Grade 2: a moderate number of root knots on the root system (6-15 cysts / plant); Grade 3: a large number of cysts on the root system (16-30 cysts / plant); Grade 4: a lot of cysts on the root system (31-50 cysts / plant); Grade 5: serious cyst situation (more than 50 cysts per plant). The disease index and control effect calculation method are the same as above. The experimental results are shown in Table 11.

[0193] Table 11

[0194]

[0195] As shown in Table 11, the lipopeptide combination has a good control effect on wheat cereal cyst nematodes, and during the test, no phytotoxicity of the combination to wheat was found, and the plants grew well. Among them, the average control effect of the lipopeptide compound combination treatment group was significantly higher than that of the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups under the same conditions, and can more effectively control plant-related nematode diseases.

[0196] The seed soaking treatment of the lipopeptide composition not only prevents and controls nematode pests, but also further promotes the growth of wheat. According to the measurement, the average plant height of the control group was 85.13 cm, and the average plant height of the plants in the single lipopeptide composition treatment group was 89.65-91.38 cm, which was 5.31% and 7.34% higher than that of the control group; the average plant height of the plants in the composition 21, 22, 26, 27, 38, 40, 53, and 57 treatment groups was 93.76-98.67 cm, and the plant height of each treatment group increased by 10.14-15.91% compared with the control group. Wheat was harvested, and the thousand-grain weight was measured after drying. The average thousand-grain weight of the single lipopeptide composition 2, 3, 5, 8, 9, and 10 treatment groups was 49.38-51.56 g, and the average thousand-grain weight of the lipopeptide compound composition treatment group was 56.37-61.83 g, which was 1.37-1.50 times that of the control group. The average plant height and 1000-grain weight of the lipopeptide compound treatment group were higher than those of the single lipopeptide compound treatment group under the same conditions, indicating that lipopeptides and their combinations can be used for plant disease prevention and control and growth promotion (see Figure 5 ).

[0197] Example 9 Evaluation of the control of common plant insect pests by lipopeptide compositions

[0198] 1. Evaluation of the control of soybean red spider mites by irrigation with lipopeptide combination

[0199] Sowing soybeans (leguminous family), the field is randomly divided into areas, each area is 60m 2 , sow in 3 areas. After sowing, the plants were watered, and the lipopeptide composition described in Example 7 was watered once every 5 days, and the amount of each watering was 200 mL, and the clear water treatment was used as a blank control. After one month of watering treatment, the growth (plant height) and insect disease incidence of soybean plants were observed. Five random sampling points were selected in each area, and 8 plants were selected at each point, for a total of 40 plants. The incidence of all leaves was investigated, graded, and the disease index and control effect were calculated.

[0200] Grading standard: Level 0: normal leaves, no visible yellow-white spots; Level 1: few yellow-white spots on each leaf (no more than 3 small spots); Level 2: fewer yellow-white spots on each leaf (4-10 small spots, 1-2 large spots); Level 3: more yellow-white spots on each leaf (10-20 small spots, 3-5 large spots); Level 4: 1 / 3 of the leaves have spots, and some leaves curl or fall off; Level 5: 2 / 3 of the leaves have spots, and some leaves curl or fall off. The disease index and control effect are calculated in the same way as above. After harvesting, the soybeans are washed and dried, and the average yield is measured and calculated. The measurement results are shown in Table 12.

[0201] Table 12

[0202]

[0203]

[0204] It was determined that the average disease index of the combination treatment group was 28.55-46.28%, and the average disease index of the control group was 68.58%, indicating that the lipopeptide treatment group had a certain control effect on soybean red spider pests, and the average control effect of the lipopeptide composite composition treatment group (43.98-58.37%) was higher than that of the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups (32.52-36.76%). After treatment, the height of soybean plants was measured. The average plant height of each treatment group was 53.29-63.27cm, and the average height of the control group was 48.15cm. The treatment group increased by 10.67-31.40% compared with the control group. Soybeans were harvested, washed and dried, and then the thousand-grain weight was measured. The treatment group increased by 3.53-18.69% compared with the control group. Among them, the growth-promoting effect of the lipopeptide composite combination treatment group was better than that of the single lipopeptide composition treatment group under the same period of time.

[0205] 2. Evaluation of the control of pumpkin aphids by irrigating with lipopeptide combination

[0206] Planting pumpkin (Cucurbitaceae), the field is randomly divided into areas, each area is 60m 2 , sowed in 3 areas. After sowing, the plants were watered, and the lipopeptide composition described in Example 7 was watered once every 5 days, and the amount of each watering was 200 mL. The clear water treatment was used as a blank control. The incidence of aphid disease in the plants was observed one month after the watering treatment. Five random sampling points were selected in each area, and 8 plants were selected at each point, for a total of 40 plants. The incidence was investigated, graded, and the disease index and control effect were calculated.

[0207] Grading standard: Level 0: No visible aphids; Level 1: Very few aphids (insect infestation range does not exceed 1%); Level 2: Few aphids (insect infestation range 1-5%); Level 3: Moderate aphids (insect infestation range 6-10%); Level 4: Large aphids, insect infestation range 11-15%; Level 5: Aphids increase significantly, insect infestation range exceeds 15%. The disease index and control effect calculation method are the same as above. Pumpkins were harvested during the harvest period, and the number and yield of pumpkins harvested in each area were counted, and the average yield was measured and calculated. The measurement results are shown in Table 13.

[0208] Table 13

[0209]

[0210]

[0211] It was determined that the average disease index of the combination treatment group was 24.32-40.37%, and the average disease index of the control group was 58.29%, indicating that the lipopeptide treatment group had a certain control effect on pumpkin aphid pests, and the average control effect of the lipopeptide composite treatment group (49.31-58.28%) was higher than that of the single lipopeptide composition 2, 3, 5, 8, 9, 10 treatment groups (29.39-32.99%). The pumpkins were harvested and the weight of each fruit was measured. The treatment group increased by 9.86-50.00% compared with the control group. Among them, the growth-promoting effect of the lipopeptide composite treatment group was better than that of the single lipopeptide composition treatment group under the same conditions (see Figure 6 ).

Claims

1. A lipopeptide composition, comprising two or three of a first lipopeptide, a second lipopeptide and a third lipopeptide, wherein the first lipopeptide comprises a peptide chain consisting of 1-7 amino acid residues, the second lipopeptide comprises a peptide chain consisting of 8-12 amino acid residues, and the third lipopeptide comprises a peptide chain consisting of 13 or more amino acid residues.

2. The lipopeptide composition according to claim 1, wherein the first lipopeptide is selected from one or more of surfactin, iturin, lichenin, mycosubtilin, fusarin, bacillus antimycin, bacitracin, babylonin, pamilasin, kurstak, agrobactin, and cyclobactin; The second lipopeptide is selected from one or more of fenmucin, phospholipid inhibitor, polymyxin, daptomycin, octapeptidin, and bacillus subtilisin; The third lipopeptide is selected from one or more of polypeptide bacteriocin and tridecapeptidin.

3. The lipopeptide composition according to claim 1 or 2, wherein the lipopeptide composition comprises two of the first lipopeptide, the second lipopeptide and the third lipopeptide, and the mass ratio of any two lipopeptides in the composition is 1:(0.02-50), preferably, the mass ratio of any two lipopeptides in the composition is 1:(0.1-10).

4. The lipopeptide composition according to claim 1 or 2, wherein the lipopeptide composition comprises three of a first lipopeptide, a second lipopeptide and a third lipopeptide, and the mass ratio of any three lipopeptides in the composition is 1:(0.02-50):(0.02-50), preferably, the mass ratio of any three lipopeptides in the composition is 1:(0.1-10):(0.1-10).

5. The lipopeptide composition according to any one of claims 1 to 4, wherein the concentration of each lipopeptide in the composition is 0.0001-300 g / L.

6. Use of the lipopeptide composition according to any one of claims 1 to 5 in preventing and controlling crop diseases or insect pests.

7. The use according to claim 6, wherein the disease is a disease caused by bacteria or a disease caused by fungi, Preferably, the bacterium is selected from one of the genus Pseudomonas, the genus Xanthomonas, the genus Erwinia and the genus Ralstonia. Preferably, the fungus is selected from one of the following: Pythium, Phytophthora, Achlya, Pasasitica, Phytophthora, Rhizopus, Penicillium, Aspergillus, Botrytis, Botryosphaeria, Fusarium, Sclerotinia, Rhizoctonia, Colletotrichum, Alterna ria), Colletotrichum, Spongospora, Venturia, Valsa, Piriformospora, Ustilagonoidea, Helminthosporium, Puccinia, Physoderma, Cercospora, Curvularia, Ustilago, Erysiphe, Gladaxporism, Septoria.

8. The use according to claim 7, wherein the pest is a nematode pest or an insect pest, Preferably, the nematode is selected from one of the following: root-knot nematodes, cyst nematodes, root-rot nematodes, root-knot nematodes, stem nematodes, semi-piercing nematodes, kidney-shaped nematodes, leaf nematodes, long-needle nematodes, Xiphinema, Preferably, the insect is selected from one of the following: Coleopteran pests, Lepidopteran pests, Hemiptera pests, Diptera pests, Isoptera pests, Blattodea pests.

9. Use of the lipopeptide composition according to any one of claims 1 to 5 as an agricultural bacteriostatic agent, agricultural insecticide, agricultural adjuvant, agricultural fertilizer or pesticide.

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