Insecticidal composition and use thereof

Abstract

The present application belongs to the technical field of pesticide combination, and particularly relates to a kind of insecticidal composition, the effective component of the insecticidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I), and the active ingredient B is a biamide insecticide, the mass ratio of the active ingredient A and the active ingredient B is 1:50-65:1.The composition of the present application has a synergistic effect at a certain ratio, can effectively control a variety of phytophagous pests, delay the development of pest resistance, and reduce the amount of pesticide used.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides and insecticides, and particularly relates to an insecticide composition and its application in preventing and controlling lepidopteran pests. Background Art

[0002] Diamide insecticides have high insecticidal activity and toxicological characteristics that prevent insects from feeding quickly. Their mechanism of action is mainly to bind to the ryanodine receptor (RyR), activate calcium ion channels, and rapidly release calcium ions from calcium stores. Calcium ions bind to troponin, causing sustained contraction of muscle fibers, paralyzing the insects and stopping feeding, ultimately leading to death from starvation.

[0003] In the actual practice of agricultural production, the use of a single pesticide can easily lead to pest resistance. Diamide insecticides provide a new chemical means of controlling lepidopteran pests, but resistance is inevitable. Various lepidopteran pests have developed varying levels of resistance, resulting in decreased field efficacy and increased dosage. Combining two or more insecticides with different structures or mechanisms of action can improve efficacy, reduce dosage, expand the control spectrum, delay the development of pest resistance, and extend the product's lifespan. In the vast majority of cases, the effects of pesticide combinations are additive, with few examples of true synergistic effects, especially those with very significant synergistic effects and high co-toxicity coefficients.

[0004] After the inventors' compounding research, the compound represented by formula (I) was rationally compounded with flufenac, tolfenpyrad, tetrazobactam, and cyantraniliprole. It was surprisingly found that the insecticidal composition had a synergistic effect under a certain mass ratio. There are no relevant reports on the compounding of the compound represented by formula (I) with flufenac, tolfenpyrad, tetrazobactam, and cyantraniliprole. Summary of the Invention

[0005] The purpose of the present invention is to provide an insecticide composition which has synergistic effect, reduces drug resistance, has low use cost and good prevention effect.

[0006] To achieve the above object, the technical solution adopted by the present invention is: an insecticide composition, wherein the active ingredients of the insecticide composition include active ingredient A and active ingredient B, and active ingredient A is a compound of formula (I) with the following structural formula:

[0007] Active ingredient B is a diamide insecticide;

[0008] Furthermore, the diamide insecticide is selected from any one of flubendiamide, tolfenpyrad, tetrazolam, and cyantraniliprole;

[0009] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 65:1;

[0010] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 50:1, or any value between the above values;

[0011] Furthermore, the mass ratio of the compound of formula (I) to flubendiamide is 1:25 to 35:1, such as 1:25, 1:15, 1:8, 9:2, 14:1, 24:1, and 35:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to flubendiamide is 1:25 to 24:1.

[0013] Furthermore, the mass ratio of the compound of formula (I) to flubendiamide is 1:25, 1:15, 1:8, 9:2, 14:1, or 24:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30 to 30:1, such as 1:50, 1:30, 1:12, 3:2, 3:1, 6:1, 12:1, 30:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30 to 12:1.

[0016] Furthermore, the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30, 1:12, 3:2, 3:1, 6:1, 12:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to tetrazolin is 1:28 to 50:1, such as 1:28, 1:14, 1:7, 3:1, 7:1, 15:1, 26:1, 50:1;

[0018] Furthermore, the mass ratio of the compound of formula (I) to tetrazolyl thiamethoxam is 1:14 to 15:1.

[0019] Furthermore, the mass ratio of the compound of formula (I) to tetrazolin is 1:14, 1:7, 3:1, 7:1, 15:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to cyantraniliprole is 1:35 to 32:1; such as 1:35, 1:25, 1:13, 5:2, 13:1, 32:1, and 65:1;

[0021] Furthermore, the mass ratio of the compound of formula (I) to cyantraniliprole is 1:25 to 32:1.

[0022] Furthermore, the mass ratio of the compound of formula (I) to cyantraniliprole is 1:25, 1:13, 5:2, 13:1, and 32:1;

[0023] Furthermore, based on the total weight of the insecticide composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the insecticide composition is 0.5 to 90 wt%;

[0024] Furthermore, based on the total weight of the insecticide composition being 100 wt%, the sum of the content of the active ingredient A and the active ingredient B in the insecticide composition is 1 to 80 wt%;

[0025] The insecticide composition of the present invention can be prepared by a common processing method known to those skilled in the art, that is, after mixing the active ingredient with a liquid solvent or a solid carrier, one or more surfactants such as a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a penetrant and a carrier are added;

[0026] Furthermore, the wetting agent is selected from a mixture of one or more of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, sapodilla powder, silkworm excrement or soapberry powder;

[0027] Furthermore, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ether phosphates, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0028] Furthermore, the emulsifier is selected from Nongru 500# (calcium alkylbenzene sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonic acid magnesium salt, triethanolamine salt, Nongru 400# (benzyl dimethylphenol polyoxyethyl ether), Nongru 700# (alkylphenol formaldehyde resin polyoxyethyl ether), Ningru 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), A mixture of one or more of the following: Nongru 1600# (phenylethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), Nongru 33# (alkylaryl polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether);

[0029] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0030] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0031] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0032] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acid compounds or C8~C 10 A mixture of one or more fatty alcohol compounds;

[0033] Furthermore, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

[0034] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0035] Furthermore, the penetrant is selected from a mixture of one or more of penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone or silicone;

[0036] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0037] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0038] The above substances are all commercially available.

[0039] The insecticide composition of the present invention can be processed into any agriculturally acceptable dosage form;

[0040] Furthermore, the insecticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0041] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0042] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0043] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0044] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; and the liquid preparation is selected from emulsifiable concentrates and suspension concentrates.

[0045] The present invention also provides the use of the above-mentioned insecticide composition in controlling lepidopteran pests;

[0046] Furthermore, the lepidopteran pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navelorangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma ta lis, Archips argyrospila (fruittree leafroller), Archips rosana (rose leafroller), and A. leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana (orange tortrix), Autographa gamma, Bonagota cranaodes, Borbo cinnara (rice leaf folder), Bucculatrix thurberiella (cotton leaf perforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peach fruit moth), Chilo spp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded rose leaf roller),

[0047] leafroller)), Chrysodeixis spp., Cnaphalocerus medinalis (grass

[0048] leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydian ignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworm), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown applemoth), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobacco moth) moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp.), Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella (grape berry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholita molesta (Oriental fruit moth).

[0049] (Oriental Fruit Moth), Hedylepta indicate (Bean Leaf Webber), Helicoverpa sp. (Spodoptera exigua), Helicoverpa armigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (Cabbage Webworm), Indarbela spp. (Root Borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (Grape Fruit Moth), fruitmoth), Loxagrotis spp., Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.) (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana (bagworm), Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis (three-spotted water borer), Operophtherabrumata (winter looper), Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus (African swallowtail), Pectinophora gossypiella (pink bollworm), (Bollworm), Peridroma species (pp.), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp., Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays olive moth oleae) (olive moth), Pseudaletia spp.) (spodoptera), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu), Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda, Spodoptera oridania (southern armyworm), Synanthedon spp., Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (cabbage worm), and Tuta absoluta;

[0050] Furthermore, the lepidopteran pests include diamondback moth, beet armyworm, Spodoptera litura, fall armyworm, striped stem borer, cotton bollworm, and cabbage worm.

[0051] The present invention also provides the above-mentioned insecticide composition to be applied in an effective dose to the pests to be controlled or the medium where they grow.

[0052] The beneficial effects of the present invention are:

[0053] 1) The insecticidal composition of the present invention has a significant control effect on lepidopteran pests at a certain mass ratio and has a synergistic effect;

[0054] 2) The insecticide composition of the present invention can significantly reduce the amount of pesticides used and delay the development of resistance of lepidopteran pests to diamide pesticides;

[0055] 3) The insecticide composition of the present invention reduces environmental pollution and is safe for humans and animals. DETAILED DESCRIPTION

[0056] The present invention is further described below with reference to the examples. The percentages in the examples are all by weight, but the present invention is not limited thereto.

[0057] The insecticide composition of the present invention can be provided in the form of a formulation. It can be formulated as a suspension concentrate, water-dispersible granules, wettable powder, or dispersible oil suspension concentrate, as needed. The content of the active ingredient in the composition of the present invention depends on the application rate when used alone, as well as the mixing ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0058] Preparation Example

[0059] Preparation Example 1:

[0060] 22% formula (Ⅰ) compound·flufenac suspension concentrate (18:4)

[0061] Preparation formula: 18% of the compound of formula (I), 4% of flufenacet, 3.5% of polyoxyethylene ether nonionic (D1109S), 3% of phenethylphenol polyether phosphate, 1% of naphthalenesulfonate (2020), 2% of dioctyl sodium sulfosuccinate, 1.2% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of glycerol, 0.02% of benzisothiazolinone, 0.5% of silicone defoamer, and the balance made up with deionized water;

[0062] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0063] Preparation Example 2

[0064] 18% formula (Ⅰ) compound·tolfenpyrad suspension concentrate (13:5)

[0065] Preparation formula: 13% of the compound of formula (I), 5% of tolfenpyrad, 3% of nonionic polyoxyethylene ether (D1109S), 3% of phenethylphenol polyether phosphate salt, 4% of dodecyl polyoxyethylene ether phosphate, 1.2% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of glycerol, 0.02% of potassium sorbate, 0.5% of organosilicon defoamer, and the balance made up with deionized water;

[0066] Preparation method: Same as Preparation Example 1.

[0067] Preparation Example 3

[0068] 28% formula (Ⅰ) compound·tetrazolylpyram suspension (21:7)

[0069] Preparation formula: 21% of the compound of formula (I), 7% of tetrazolyl amide, 3% of phenylethylphenol polyether phosphate, 2.5% of sodium dioctyl sulfosuccinate, 2% of polyoxyethylene ether nonionic (D1109S), 1.2% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of glycerol, 0.02% of potassium sorbate, 0.5% of organic silicon defoamer, and the balance made up of deionized water;

[0070] Preparation method: Same as Preparation Example 1.

[0071] Preparation Example 4

[0072] 28% formula (Ⅰ) compound·cyantraniliprole suspension concentrate (20:8)

[0073] Preparation formula: 20% of the compound of formula (I), 8% of cyantraniliprole, 2.5% of castor oil polyoxyethylene ether (110), 3% of phenethylphenol polyether phosphate, 2% of sodium dioctyl sulfosuccinate, 1.2% of magnesium aluminum silicate, 0.25% of xanthan gum, 4.5% of glycerol, 0.02% of benzisothiazolinone, 0.5% of organosilicon defoamer, and the balance is made up of deionized water;

[0074] Preparation method: Same as Preparation Example 1.

[0075] Preparation Example 5

[0076] 8% formula (Ⅰ) compound·tetrazolylcarb dispersible oil suspension (7:1)

[0077] Preparation formula: 7% of the compound of formula (I), 1% of tetrazolyl thiamethoxam, 2% of succinate sulfonate, 12% of alkylaryl polyoxyethylene polyoxypropylene ether, 3% of fatty alcohol polyoxyethylene ether, 3% of calcium dodecylbenzenesulfonate, 1.5% of silicon dioxide, 0.5% of organic bentonite, 15% of 200# solvent oil, and the balance made up of methyl oleate;

[0078] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, and solvent oil is added and mixed evenly. After high-speed shearing, wet sand grinding, and finally homogenization filtration, the dispersible oil suspension product is obtained.

[0079] Preparation Example 6

[0080] 47% formula (Ⅰ) compound·cyantraniliprole wettable powder (32:15)

[0081] Preparation formula: 32% of the compound of formula (I), 15% of cyantraniliprole, 4.5% of alkyl naphthalene sulfonate (Morwet EFW), 4% of a block copolymer dispersant (D-800), 3% of sodium alkyl polyoxyethylene ether sulfonate, 3.5% of sodium lignin sulfonate, and the balance made up of kaolin;

[0082] Preparation method: The active ingredients, other functional additives and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and crushed and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0083] Preparation Example 7

[0084] 42% Formula (I) compound·Flufenac water dispersible granules (32:10)

[0085] Preparation formula: 32% of the compound of formula (I), 10% of flubendiamide, 7% of sodium lignin sulfonate (WG4), 7% of naphthalene sulfonate formaldehyde condensate, 2% of sodium lauryl sulfate, 4.5% of white carbon black, 20% of starch, and kaolin as the balance;

[0086] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and screening.

[0087] Preparation Example 8

[0088] 50% formula (Ⅰ) compound·tolfenpyrad water dispersible granules (35:15)

[0089] Preparation formula: 35% of the compound of formula (I), 15% of tolfenpyrad, 7.5% of sodium lignin sulfonate (WG4), 8.5% of naphthalene sulfonate formaldehyde condensate, 2% of sodium lauryl sulfate, 5% of white carbon black, 24% of starch, and kaolin as the balance;

[0090] Preparation method: Same as Preparation Example 7.

[0091] Indoor biological activity assay:

[0092] The specific test methods are as follows:

[0093] Lepidoptera larvae: a combination of leaf and insect dipping;

[0094] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and consistent Lepidoptera 2nd instar larvae (≥60 per treatment).

[0095] Experimental treatment: Use tweezers to place fresh cabbage slices in the drug solution and immerse them for 10 seconds, then take them out. After the drug solution is naturally dried, place the cabbage slices in a culture dish lined with moisturizing filter paper. After placing the test insects in the drug solution for 5 seconds, use filter paper to absorb the excess drug solution and place the test insects in a culture dish with leaves soaked in the corresponding concentration.

[0096] Each treatment was repeated 4 times, with a blank treatment as a control. The treated insects were reared in an artificial intelligence culture chamber at 26±1°C, a light intensity of L:D=16h:8h, and a relative humidity of 60%.

[0097] After treatment with the pesticide, the mortality of the test insects was investigated 48 hours later. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0098] Data statistics and analysis:

[0099] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula:

[0100]

[0101] Where:

[0102] P——mortality rate, in percentage (%);

[0103] K——indicates the number of dead insects, the unit is head;

[0104] N——represents the total number of insects processed, in heads.

[0105]

[0106] Where:

[0107] P1——adjusted mortality rate, in percentage (%);

[0108] P t ——Treatment mortality rate, expressed in percentage (%);

[0109] P0 - blank control mortality rate, in percentage (%).

[0110] Data statistics and analysis: If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made; if the control mortality rate is greater than 20%, the experiment needs to be repeated.

[0111] The data were processed by probability value analysis method and analyzed by DPS statistical analysis system to obtain the toxicity regression line, LC 50 The activity of the test agents on the biological materials was evaluated by using the values, 95% confidence limits and correlation coefficient r.

[0112] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0113]

[0114] Where:

[0115] ATI - measured toxicity index of mixture;

[0116] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0117] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0118] TTI=TI A * P A +TI B * P B

[0119] Where:

[0120] TTI – Theoretical Toxicity Index of Mixtures;

[0121] TI A ——Agent toxicity index;

[0122] P A ——The percentage of agent A in the mixture, in percentage (%);

[0123] TI B ——Toxicity index of agent B;

[0124] P B ——The percentage of agent B in the mixture, in percentage (%).

[0125]

[0126] Where:

[0127] CTC – Co-toxicity coefficient;

[0128] ATI - measured toxicity index of mixture;

[0129] TTI - Theoretical Toxicity Index of Mixture.

[0130] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0131] Indoor activity determination of Plutella xylostella

[0132] As shown in Table 1, flubendiamide has a high toxicity to Plutella xylostella, and its LC 50 The LC value of the compound of formula (I) against diamondback moth is 0.394 mg / L. 50 It is 9.036 mg / L.

[0133] When the weight ratio of the compound of formula (I) and flubendiamide to the diamondback moth is 1:25 to 35:1, the co-toxicity coefficient is greater than 120, and the mixture of the two shows a synergistic effect within this weight ratio range; when the weight ratio of the compound of formula (I) and flubendiamide to the diamondback moth is 1:25 to 24:1, the co-toxicity coefficient is greater than 140, which has an obvious synergistic effect.

[0134] Table 1 Toxicity test results of compound of formula (I) and flubendiamide against diamondback moth

[0135]

[0136] As shown in Table 2, tolfenpyrad has a high toxicity to Plutella xylostella, and its LC 50 The LC value of the compound of formula (I) against diamondback moth is 1.505 mg / L. 50 It is 9.036 mg / L.

[0137] When the weight ratio of the compound of formula (I) and tolfenpyrad to the diamondback moth is 1:50 to 30:1, the co-toxicity coefficient is greater than 80, and the mixing of the two within the range of 1:50 to 30:1 shows an additive or synergistic effect, and no antagonistic effect occurs; when the weight ratio of the compound of formula (I) and tolfenpyrad to the diamondback moth is 1:30 to 30:1, the co-toxicity coefficient is greater than 130, and the mixing of the two within this weight ratio range shows a synergistic effect.

[0138] Table 2 Toxicity test results of compound of formula (I) combined with tolfenpyrad against Plutella xylostella

[0139]

[0140] Indoor activity measurement of Pieris rapae

[0141] As shown in Table 3, tetrazobactam has a high toxicity to Pieris rapae, and its LC 50 The LC value of the compound of formula (I) against Pieris rapae is 0.456 mg / L. 50 It is 12.776 mg / L.

[0142] When the weight ratio of the compound of formula (I) and tetrazopyram to Pieris rapae is 1:28 to 50:1, the co-toxicity coefficient is greater than 120, and the mixture of the two shows a synergistic effect within this weight ratio range.

[0143] Table 3 Toxicity test results of compound of formula (I) and tetrazopyram against Pieris rapae

[0144]

[0145] As shown in Table 4, cyantraniliprole has a high toxicity to Pieris rapae, and its LC 50The LC value of the compound of formula (I) against Pieris rapae is 0.590 mg / L. 50 It is 12.776 mg / L.

[0146] When the weight ratio of the compound of formula (I) and cyantraniliprole to Pieris rapae is 1:35 to 65:1, the co-toxicity coefficient is greater than 80. The mixing of the two in the range of 1:35 to 65:1 shows an additive or synergistic effect, and no antagonistic effect occurs; when the weight ratio of the compound of formula (I) and cyantraniliprole to Pieris rapae is 1:35 to 32:1, the co-toxicity coefficient is greater than 120, indicating that the mixing of the two shows a synergistic effect within this weight ratio range.

[0147] Table 4 Toxicity test results of compound of formula (I) combined with cyantraniliprole against Pieris rapae

[0148]

[0149] Field trials on controlling diamondback moth

[0150] Test crops: cauliflower (Qingsong 65);

[0151] Test target: Diamondback moth;

[0152] Test reference: Based on GB / T 17980.13-2000 "Guidelines for field efficacy tests (I): Insecticides for the control of lepidopteran larvae of cruciferous vegetables";

[0153] Experimental location: Experimental field of cruciferous vegetable planting base in Shuiquan Village, Shilibao Town, Miyun District, Beijing;

[0154] Application equipment: 3WBS-16 electric backpack sprayer;

[0155] Experimental crops: Cauliflower was planted on July 20, 2023, with a row spacing of 0.5 m × plant spacing of 0.3 m. All experimental plots were cultivated under uniform conditions, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil.

[0156] Test date: September 2, 2023 (cauliflower rosette stage);

[0157] Test agent:

[0158] Table 5 Field test design and dosage

[0159]

[0160] Test method: Apply the pesticide once during the peak period of diamondback moth infestation.

[0161] Plot size and repetition: random block arrangement, plot size 20m 2 , each treatment was repeated 4 times.

[0162] Survey method: Five random sampling points were selected in each plot, and two cauliflower plants were fixed at each point. The number of diamondback moths on the whole cauliflower plant was investigated. The base number of insects was investigated before spraying, and the number of remaining insects was investigated 3 days and 7 days after spraying.

[0163] Calculation formula and data analysis:

[0164]

[0165] Test results:

[0166] The results in Table 6 show that 3 days after application, 22% of the compound of formula (I)·flufenacil suspension concentrate (18:4) and 18% of the compound of formula (I)·tolfenpyrad suspension concentrate (13:5) showed good quick-acting properties against Plutella xylostella, with control efficacies of 86.51% and 86.17%, respectively, which were significantly higher than the control single agent.

[0167] Table 6 Effects of different pesticides on the control of diamondback moth in the field (3 days after application)

[0168]

[0169] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0170] The results in Table 7 show that 7 days after application, 22% of the formula (I) compound·flufenacil suspension concentrate (18:4) and 18% of the formula (I) compound·tolfenpyrad suspension concentrate (13:5) showed good persistent effect on Plutella xylostella, with control efficacy of 91.66% and 90.87%, respectively, which were significantly higher than the control single agent.

[0171] Table 7 Field control effects of different pesticides on Plutella xylostella (7 days after application)

[0172]

[0173] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0174] Safety: During the trial period and subsequent observations, cauliflower grew normally and no obvious pesticide damage was observed.

[0175] Field trials on controlling cabbage looper

[0176] Experimental crops: Cabbage (cabbage), which was in the rosette stage during the experiment;

[0177] Test target: Pieris rapae;

[0178] Test reference: Based on GB / T 17980.13-2000 "Guidelines for field efficacy tests (I): Insecticides for the control of lepidopteran larvae of cruciferous vegetables";

[0179] Experimental location: Cabbage experimental field in the cruciferous vegetable planting base of Biejiatun Village, Anqiu City, Weifang City, Shandong Province;

[0180] Application equipment: 3WBS-16 electric backpack sprayer;

[0181] Trial date: July 12, 2023;

[0182] Experimental environment: All experimental plots were uniformly cultivated, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil;

[0183] Test agent:

[0184] Table 8 Field test design and dosage

[0185]

[0186] Test method: Apply the pesticide once during the peak period of cabbage looper occurrence (mostly 2nd to 4th instar).

[0187] Plot size and repetition: random block arrangement, plot size 20m 2 , each treatment was repeated 4 times.

[0188] Survey method: Randomly sample five points in each plot, hang a sign on four cabbage plants at each point, investigate the number of cabbage loopers on all cabbages, investigate the base number of insects before spraying, and investigate the number of remaining insects 3 days and 14 days after spraying.

[0189] Calculation formula and data analysis:

[0190]

[0191] Test results:

[0192] The results in Table 9 show that 3 days after application, 28% of the compound of formula (I)·tetrazobactam suspension concentrate (21:7) and 28% of the compound of formula (I)·cyantraniliprole suspension concentrate (20:8) showed good quick-acting properties against cabbage loopers, with control rates of 88.19% and 86.23%, respectively, which were significantly higher than the control single agent.

[0193] Table 9 Field control effects of different pesticides on cabbage worms (3 days after application)

[0194]

[0195] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0196] The results in Table 10 show that 14 days after application, 28% of the compound of formula (I)·tetrazobactam suspension concentrate (21:7) and 28% of the compound of formula (I)·cyantraniliprole suspension concentrate (20:8) showed good persistent effect on cabbage loopers, with control efficacy of 94.85% and 93.68%, respectively, which were significantly higher than the control single agent.

[0197] Table 10 Effects of different pesticides on the control of cabbage worms in the field (14 days after application)

[0198]

[0199]

[0200] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0201] Safety: During the test period and later observations, cabbage grew normally and no obvious phytotoxicity was observed.

[0202] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition, characterized in that The active ingredients of the insecticide composition include active ingredient A and active ingredient B. Active ingredient A is a compound of formula (I) with the following structural formula: Formula (I); Active ingredient B is a diamide insecticide, and the diamide insecticide is selected from any one of fluchlorfenapyr, tolfenpyrad, tetrazolyl thiophanate, and cyantraniliprole. The mass ratio of the compound of formula (I) to fluchlorfenapyr is 1:25~35:1, the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30~30:1, the mass ratio of the compound of formula (I) to tetrazolyl thiophanate is 1:28~50:1, and the mass ratio of the compound of formula (I) to cyantraniliprole is 1:35~32:

1.

2. The insecticidal composition according to claim 1, characterized in that The mass ratio of the compound of formula (I) to flufenacet is 1:25-24:1, the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30-12:1, the mass ratio of the compound of formula (I) to tetrazopyrad is 1:14-15:1, and the mass ratio of the compound of formula (I) to cyantraniliprole is 1:25-32:

1.

3. The insecticidal composition according to claim 1, characterized in that The mass ratio of the compound of formula (I) to flufenacet is 1:25, 1:15, 1:8, 9:2, 14:1, and 24:1; the mass ratio of the compound of formula (I) to tolfenpyrad is 1:30, 1:12, 3:2, 3:1, 6:1, and 12:1; the mass ratio of the compound of formula (I) to tetrazopyrad is 1:14, 1:7, 3:1, 7:1, and 15:1; and the mass ratio of the compound of formula (I) to cyantraniliprole is 1:25, 1:13, 5:2, 13:1, and 32:

1.

4. The insecticidal composition according to claim 1, characterized in that Based on the total weight of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5 to 90 wt%.

5. The insecticidal composition according to claim 4, characterized in that Based on the total weight of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1 to 80 wt%.

6. The insecticidal composition according to claim 1, characterized in that The insecticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a stabilizer, a penetrant and a carrier.

7. The insecticidal composition according to claim 6, characterized in that The insecticide composition is prepared into a formulation form permitted in agriculture, and the formulation form is a wettable powder, a water-dispersible granule, a suspension or a dispersible oil suspension.

8. Use of the insecticide composition according to any one of claims 1 to 7 in controlling agricultural, forestry or horticultural herbivorous pests, characterized in that: The herbivorous pests are diamondback moth and cabbage looper.

9. The use according to claim 8, characterized in that The insecticide composition is applied to the pests to be controlled or the medium where they grow in an effective dose.

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