A traditional Chinese medicine composition for treating stroke

Abstract

The present invention relates to a traditional Chinese medicine composition for treating stroke. The traditional Chinese medicine composition is prepared from 10 - 30 parts by weight of Poria cocos, 5 - 25 parts by weight of Uncaria rhynchophylla, 5 - 25 parts by weight of Salvia miltiorrhiza, 3 - 18 parts by weight of Citrus reticulata Blanco, 3 - 18 parts by weight of Gastrodia elata Blume, 3 - 18 parts by weight of Ligusticum chuanxiong Hort., 3 - 18 parts by weight of Pinellia ternata (Thunb.) Breit., 1 - 15 parts by weight of Arisaema cum Bile, and 1 - 15 parts by weight of Carthamus tinctorius L. The traditional Chinese medicine composition of the present invention resolves phlegm and extinguishes wind, promotes blood circulation and dredges collaterals, has high safety, and has remarkable curative effects on treating stroke, especially acute ischemic stroke.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine, and particularly to a traditional Chinese medicine composition for treating stroke. Background Art

[0002] Stroke is the second leading cause of death globally and has become the first leading cause of death in China. Ischemic stroke (IS) is a disorder of cerebral blood circulation, resulting in focal cerebral ischemic necrosis or softening due to ischemia and hypoxia, and presenting corresponding neurological deficit symptoms. Ischemic stroke accounts for about 70% of all strokes, and currently, the annual incidence of ischemic stroke in China is increasing at a rate of 8.7%. Facing a large patient population and increasing social medical expenses, it is of great significance to prevent and treat this disease.

[0003] Currently, the main Western medical treatments for ischemic stroke include vascular recanalization treatments such as intravenous thrombolysis, thrombectomy, and endovascular treatment. In addition, neuroprotective agents are also an effective treatment method, mainly including calcium antagonists, free radical scavengers, and cell membrane stabilizers. However, the existing treatment methods have multiple limitations. First, due to the time window limitation, these methods are only applicable to a small number of patients. Second, these methods may increase the risk of hemorrhagic transformation in patients, including intracranial hemorrhage, and in severe cases, can accelerate the death of patients. Therefore, there is an urgent need in this field for a widely applicable treatment method for ischemic stroke.

[0004] Stroke belongs to the category of "stroke disease" in traditional Chinese medicine. The ancient literature that first described the symptoms of stroke disease is "Huangdi Neijing", which mentions terms such as "sudden syncope", "major syncope", "lateral wind", "hemiplegia", etc., all of which are disease names describing the symptoms of "stroke". The medical monograph that first named symptoms such as "hemiplegia, numbness, and speech impairment" as "stroke disease" is Zhang Zhongjing's "Synopsis of the Golden Chamber". Modern traditional Chinese medicine scholars have put forward their respective views through multiple studies and synthesized the etiology and pathogenesis of stroke disease into 7 categories: "wind, fire, phlegm, stasis, qi, deficiency, and toxin".

[0005] Ischemic stroke has an acute onset and a changeable condition. The traditional Chinese medicine syndromes are complex and often have concurrent syndromes. Research has found that in the acute stage of ischemic stroke, excess syndromes are predominant, mainly wind syndrome, phlegm-dampness syndrome, and blood stasis syndrome, and there are often combined syndrome types, with wind-phlegm, fire-phlegm, wind-fire, phlegm-stasis, wind-fire-phlegm, and wind-fire-stasis being more common; in the recovery stage, excess syndromes are mainly heat and phlegm-dampness, and deficiency syndromes are mainly qi deficiency; in the sequela stage, the syndrome types are mainly blood stasis, internal wind, and deficiency syndromes, and qi deficiency and blood stasis syndrome is the main syndrome type. Whether in the acute stage or the recovery stage, the method of promoting blood circulation to remove blood stasis is widely accepted by clinicians. The traditional Chinese medicine decoction is mainly Xuefu Zhuyu Decoction and Tongqiao Huoxue Decoction; the traditional Chinese medicine injection extracted from drugs for promoting blood circulation to remove blood stasis is also widely used in the acute stage treatment due to its fast absorption and rapid action.

[0006] At present, there is no relevant report on traditional Chinese medicine formula that can safely and effectively treat acute ischemic stroke. Summary of the Invention

[0007] To solve the above technical problems, the present invention includes the following aspects:

[0008] The first aspect of the present invention provides a traditional Chinese medicine composition for treating stroke, which is made from Poria cocos, Uncaria rhynchophylla, Salvia miltiorrhiza, Citrus reticulata Blanco, Gastrodia elata Blume, Ligusticum chuanxiong Hort., Pinellia ternata (Thunb.) Breit., Arisaema erubescens (Wall.) Schott and Carthamus tinctorius L.

[0009] Preferably, the traditional Chinese medicine composition is made from 10-30 parts by weight of Poria cocos, 5-25 parts by weight of Uncaria rhynchophylla, 5-25 parts by weight of Salvia miltiorrhiza, 3-18 parts by weight of Citrus reticulata Blanco, 3-18 parts by weight of Gastrodia elata Blume, 3-18 parts by weight of Ligusticum chuanxiong Hort., 3-18 parts by weight of Pinellia ternata (Thunb.) Breit., 1-15 parts by weight of Arisaema erubescens (Wall.) Schott and 1-15 parts by weight of Carthamus tinctorius L.

[0010] More preferably, the traditional Chinese medicine composition is made from 15-25 parts by weight of Poria cocos, 10-20 parts by weight of Uncaria rhynchophylla, 10-20 parts by weight of Salvia miltiorrhiza, 5-15 parts by weight of Citrus reticulata Blanco, 5-15 parts by weight of Gastrodia elata Blume, 5-15 parts by weight of Ligusticum chuanxiong Hort., 5-15 parts by weight of Pinellia ternata (Thunb.) Breit., 3-10 parts by weight of Arisaema erubescens (Wall.) Schott and 3-10 parts by weight of Carthamus tinctorius L.

[0011] Further preferably, the traditional Chinese medicine composition is made from 18-22 parts by weight of Poria cocos, 12-18 parts by weight of Uncaria rhynchophylla, 12-18 parts by weight of Salvia miltiorrhiza, 8-12 parts by weight of Citrus reticulata Blanco, 8-12 parts by weight of Gastrodia elata Blume, 8-12 parts by weight of Ligusticum chuanxiong Hort., 7-11 parts by weight of Pinellia ternata (Thunb.) Breit., 4-8 parts by weight of Arisaema erubescens (Wall.) Schott and 4-8 parts by weight of Carthamus tinctorius L.

[0012] Most preferably, the traditional Chinese medicine composition is made from 20 parts by weight of Poria cocos, 15 parts by weight of Uncaria rhynchophylla, 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Citrus reticulata Blanco, 10 parts by weight of Gastrodia elata Blume, 10 parts by weight of Ligusticum chuanxiong Hort., 9 parts by weight of Pinellia ternata (Thunb.) Breit., 6 parts by weight of Arisaema erubescens (Wall.) Schott and 6 parts by weight of Carthamus tinctorius L.

[0013] Preferably, the traditional Chinese medicine composition further includes pharmaceutically acceptable excipients.

[0014] Preferably, the traditional Chinese medicine composition is an oral preparation.

[0015] Preferably, the dosage form of the traditional Chinese medicine composition is tablets, capsules, granules, tinctures or decoctions.

[0016] More preferably, the dosage form of the traditional Chinese medicine composition is decoction.

[0017] Preferably, the preparation method of the traditional Chinese medicine composition comprises the following steps: taking the prescribed amounts of Poria cocos, Uncaria rhynchophylla, Salvia miltiorrhiza, Citrus reticulata Blanco, Gastrodia elata Blume, Ligusticum wallichii Franch., Pinellia ternata (Thunb.) Breit., Arisaema heterophyllum Blume and Carthamus tinctorius, adding water 3 - 5 times the total weight of the Chinese medicinal materials, boiling over high heat and then simmering for 30 minutes, filtering, and concentrating the traditional Chinese medicine filtrate to the weight of the initially added total weight of the Chinese medicinal materials, and optionally adding pharmaceutically acceptable excipients to obtain the composition.

[0018] Preferably, in the above preparation method, water 4 times the total weight of the Chinese medicinal materials is added.

[0019] The second aspect of the present invention provides a preparation method of the above traditional Chinese medicine composition, which comprises the following steps: taking the prescribed amounts of Poria cocos, Uncaria rhynchophylla, Salvia miltiorrhiza, Citrus reticulata Blanco, Gastrodia elata Blume, Ligusticum wallichii Franch., Pinellia ternata (Thunb.) Breit., Arisaema heterophyllum Blume and Carthamus tinctorius, adding water 3 - 5 times the total weight of the Chinese medicinal materials, boiling over high heat and then simmering for 30 minutes, filtering, and concentrating the traditional Chinese medicine filtrate to the weight of the initially added total weight of the Chinese medicinal materials, and optionally adding pharmaceutically acceptable excipients to obtain the composition.

[0020] Preferably, in the above preparation method, water 4 times the total weight of the Chinese medicinal materials is added.

[0021] The third aspect of the present invention provides the application of the above traditional Chinese medicine composition in the preparation of a medicament for treating stroke.

[0022] Preferably, the stroke is ischemic stroke. More preferably, the ischemic stroke is acute ischemic stroke.

[0023] The fourth aspect of the present invention provides the application of the above traditional Chinese medicine composition in the preparation of a medicament for reducing the content of brain inflammatory factors.

[0024] Preferably, the inflammatory factors are TNF-α and / or IL-β.

[0025] The common pharmacological effects of the traditional Chinese medicine raw materials selected in the present invention are as follows:

[0026] Poria cocos: sweet, light in flavor, neutral in nature, entering the heart, spleen and kidney meridians. Poria cocos promotes diuresis and eliminates dampness, strengthens the spleen and harmonizes the stomach, soothes the mind and calms the nerves, and is used for treating dampness syndromes such as dysuria, edema, phlegm-fluid retention, cough, poor appetite, abdominal distension, diarrhea, dizziness, palpitation, insomnia, spermatorrhea, spermatorrhea with turbid discharge, urinary dribbling, leukorrhea, etc.

[0027] Uncaria rhynchophylla: sweet in flavor, cool in nature, belonging to the liver and pericardium meridians. Uncaria rhynchophylla clears heat and calms the liver, extinguishes wind and stops convulsions, and is used for treating convulsions, high fever with convulsions, common cold with convulsions, infantile frightened crying, eclampsia during pregnancy, headache and dizziness, etc.

[0028] Salvia miltiorrhiza: bitter, slightly cold, belonging to the heart and liver meridians. Salvia miltiorrhiza dispels stasis and relieves pain, promotes blood circulation and dredges meridians, clears the heart and removes vexation, and is used for treating irregular menstruation, amenorrhea and dysmenorrhea, masses and accumulations, stabbing pain in the chest and abdomen, arthralgia due to heat pathogen, swelling and pain of sores and ulcers, restlessness and insomnia, hepatosplenomegaly, angina pectoris, etc.

[0029] Tangerine Peel: Bitter, pungent, warm; acts on the lung and spleen meridians. Tangerine peel regulates qi and strengthens the spleen, dries dampness and reduces phlegm, and is used to treat chest and epigastric fullness, anorexia with vomiting and diarrhea, cough with profuse phlegm, etc.

[0030] Gastrodia Rhizome: Sweet, pungent, neutral; acts on the liver meridian. Gastrodia rhizome extinguishes wind and stops convulsions, calms the liver-yang, dispels wind and dredges collaterals, and is used to treat acute and chronic infantile convulsions, spasm and contracture, tetanus, dizziness, headache, hemiplegia, limb numbness, rheumatic arthralgia, etc.

[0031] Chuanxiong Rhizome: Warm in nature, pungent in taste; acts on the liver, gallbladder, and pericardium meridians. Chuanxiong rhizome promotes blood circulation and qi movement, dispels wind and relieves pain, and is used to treat chest pain due to chest impediment, stabbing pain in the chest and hypochondrium, swelling and pain from falls and bruises, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to mass in the abdomen, headache, rheumatic arthralgia, etc.

[0032] Pinellia Tuber: Pungent in taste, warm in nature; acts on the spleen, stomach, and lung meridians. Pinellia tuber dries dampness and reduces phlegm, suppresses the adverse flow of qi and stops vomiting, dissipates binds and resolves masses, and is used to treat damp phlegm and cold phlegm, cough with profuse phlegm, dizziness and palpitation due to phlegm retention, wind phlegm dizziness, headache due to phlegm syncope, vomiting and nausea, fullness and oppression in the chest and epigastrium, globus hystericus, etc.

[0033] Arisaema Cum Bile: Bitter, slightly pungent, cool; acts on the lung, liver, and spleen meridians. Arisaema cum bile clears heat and reduces phlegm, extinguishes wind and calms fright, and is used to treat cough with yellow and thick phlegm due to phlegm-heat, syncope due to wind-phlegm, mania and epilepsy, etc.

[0034] Safflower: Warm in nature, pungent in taste; acts on the heart and liver meridians. Safflower promotes blood circulation to dredge the meridians, dispels stasis and relieves pain, and is used to treat amenorrhea, dysmenorrhea, retention of lochia, mass in the abdomen, swelling and pain from falls and bruises, sores and ulcers, etc.

[0035] Technical effects produced by the present invention:

[0036] The formula compatibility of the traditional Chinese medicine composition of the present invention is reasonable. The inventor has obtained the preferred components and content ratios of the traditional Chinese medicine composition of the present invention through a large number of experiments. The traditional Chinese medicine composition of the present invention reduces phlegm and extinguishes wind, promotes blood circulation and dredges collaterals, has high safety, and has a significant effect on the treatment of stroke, especially acute ischemic stroke. Specific embodiments

[0037] The following further illustrates the present invention in conjunction with embodiments, but the embodiments of the present invention are not limited thereto. The experimental methods used in the following embodiments are all conventional methods unless otherwise specified.

[0038] Example 1

[0039] Take 20 g of Poria, 15 g of Uncaria Rhynchophylla, 15 g of Salvia Miltiorrhiza, 10 g of Tangerine Peel, 10 g of Gastrodia Rhizome, 10 g of Chuanxiong Rhizome, 9 g of Pinellia Tuber, 6 g of Arisaema Cum Bile, and 6 g of Safflower, add 400 mL of pure water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction 1 of the present invention.

[0040] Example 2

[0041] Take 10 g of Poria cocos, 20 g of Uncaria rhynchophylla, 20 g of Salvia miltiorrhiza, 10 g of Citrus reticulata Blanco, 10 g of Gastrodia elata Blume, 10 g of Ligusticum chuanxiong Hort., 9 g of Pinellia ternata (Thunb.) Breit., 6 g of Arisaema heterophyllum Blume, and 6 g of Carthamus tinctorius L., add 400 mL of purified water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction 2 of the present invention.

[0042] Example 3

[0043] Take 20 g of Poria cocos, 10 g of Uncaria rhynchophylla, 10 g of Salvia miltiorrhiza, 15 g of Citrus reticulata Blanco, 15 g of Gastrodia elata Blume, 10 g of Ligusticum chuanxiong Hort., 9 g of Pinellia ternata (Thunb.) Breit., 6 g of Arisaema heterophyllum Blume, and 6 g of Carthamus tinctorius L., add 400 mL of purified water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction 3 of the present invention.

[0044] Example 4

[0045] Take 20 g of Poria cocos, 10 g of Uncaria rhynchophylla, 10 g of Salvia miltiorrhiza, 10 g of Citrus reticulata Blanco, 10 g of Gastrodia elata Blume, 15 g of Ligusticum chuanxiong Hort., 14 g of Pinellia ternata (Thunb.) Breit., 6 g of Arisaema heterophyllum Blume, and 6 g of Carthamus tinctorius L., add 400 mL of purified water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction 4 of the present invention.

[0046] Comparative Example 1 (Poria cocos replaced with Alisma orientale (Sam.) Juz.)

[0047] Take 20 g of Alisma orientale (Sam.) Juz., 15 g of Uncaria rhynchophylla, 15 g of Salvia miltiorrhiza, 10 g of Citrus reticulata Blanco, 10 g of Gastrodia elata Blume, 10 g of Ligusticum chuanxiong Hort., 9 g of Pinellia ternata (Thunb.) Breit., 6 g of Arisaema heterophyllum Blume, and 6 g of Carthamus tinctorius L., add 400 mL of purified water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction of Comparative Example 1.

[0048] Comparative Example 2 (Ligusticum chuanxiong Hort. replaced with Panax notoginseng (Burkill) F. H. Chen ex Tsai & Tseng)

[0049] Take 20 g of Poria cocos, 15 g of Uncaria rhynchophylla, 15 g of Salvia miltiorrhiza, 10 g of Citrus reticulata Blanco, 10 g of Gastrodia elata Blume, 10 g of Panax notoginseng (Burkill) F. H. Chen ex Tsai & Tseng, 9 g of Pinellia ternata (Thunb.) Breit., 6 g of Arisaema heterophyllum Blume, and 6 g of Carthamus tinctorius L., add 400 mL of purified water, bring to a boil over high heat and then simmer for 30 minutes, filter, and concentrate the traditional Chinese medicine filtrate to 100 mL to obtain the traditional Chinese medicine decoction of Comparative Example 2.

[0050] Experimental Example 1. Cerebral protection effect of the traditional Chinese medicine composition of the present invention on a rat model of acute ischemic stroke

[0051] 1. Test method

[0052] 1.1. Construction of the tMCAO rat model

[0053] The rats were anesthetized with a gas anesthesia machine and fixed in a supine position on the operating table. The skin was longitudinally incised along the midline of the neck, and the right common carotid artery (CCA), external carotid artery (ECA), and internal carotid artery (ICA) were carefully dissected. The ECA was ligated and transected, and then straightened to form a straight line with the ICA. A small incision was made on the ECA, and a round-tipped siliconized nylon thread with a length of 4.0 cm and a diameter of 0.26 mm was inserted through this opening into the ICA for about 2 cm to the origin of the anterior cerebral artery of the rat, blocking the blood flow supply of the middle cerebral artery, and monitoring cerebral blood flow. After 2 hours of ischemia, the nylon thread was carefully withdrawn, the opening of the ECA was ligated, and the surgical incision was sutured.

[0054] 1.2. Experimental grouping and dosing regimen

[0055] The rats after tMCAO were randomly divided into 7 groups, namely the model group, the groups of Examples 1-4 of the present invention, and the groups of Comparative Examples 1-2, with 10 rats in each group. Except for the model group, the rats in the remaining experimental groups were orally administered the decoctions prepared in Examples 1-4 and Comparative Examples 1-2 of the present invention at a dose of 1 mL / kg within 5 minutes after tMCAO ischemia surgery, and reperfusion was carried out 120 minutes after ischemia surgery. After 24 hours of reperfusion, behavioral evaluation and TTC staining were performed, and the cerebral infarction volume and biochemical determination of brain tissue inflammatory factors were analyzed and statistically analyzed.

[0056] 1.3. Neurological behavior scoring of tMCAO rats

[0057] The behavioral observation of the animals was carried out 24 hours after ischemia reperfusion in rats. A four-level scoring method (0-4 points) was used: ① The behavior was completely normal at 0 points; ② When the rat's tail was lifted off the ground and the contralateral forelimb of the surgery was internally rotated and adducted, it was 1 point; ③ When the rat was placed on the ground and both sides were squeezed by hand to check its resistance, and the resistance on the contralateral side of the surgery decreased, it was 2 points; ④ When the rat was placed on the ground and its walking was observed, and it circled around the contralateral side of the surgery, it was 3 points; ⑤ When the injury was extremely severe and it could no longer move independently, it was 4 points. The higher the score, the more severe the neurological behavior injury.

[0058] 1.4. Determination of cerebral infarction volume in tMCAO rats

[0059] 24 hours after ischemia reperfusion in rats, the head was immediately cut off to remove the olfactory tract, cerebellum, and lower brainstem, and the brain was coronally cut into 6 slices (the first to fifth slices were 2 mm thick each, and the sixth slice was 4 mm thick), and quickly placed in 5 mL containing 4% TTC (1.5 mL) and 1 mol·L -1Stain in a solution of K2HPO4 (0.1 mL) (at 37 °C, protected from light) for 20 - 30 min, and turn it over once every 5 min during this period. After TTC staining, normal tissues are stained dark red, and infarcted tissues are white. Arrange the brain slices of each group neatly, take pictures and save them. Use the image analysis system software Photoshop to process and count, calculate the infarct area of each slice, and finally superimpose and convert it into infarct volume. The infarct volume is expressed as the percentage of the cerebral hemisphere occupied to eliminate the influence of brain edema. Cerebral infarction volume ratio (%) = (volume of the contralateral hemisphere of the operation - volume of the non-infarcted part of the operative hemisphere) / volume of the contralateral hemisphere of the operation × 100%.

[0060] 1.5. Determination of biochemical tissues such as brain tissue inflammatory factors

[0061] Decapitate the rats in each experimental group to obtain the damaged side of the half brain, dry it with filter paper and weigh it. Add normal saline according to the weight-volume ratio of 1:9, and prepare 10% tissue homogenate with a homogenizer. Then centrifuge for 10 min and take the supernatant. According to the instructions of the TNF-α and IL-β kits, measure the content of brain tissue inflammatory factors.

[0062] 2. Experimental results

[0063] 2.1. Results of rat neurobehavioral score and determination of cerebral infarction volume percentage

[0064] The effects of the traditional Chinese medicine decoctions in each experimental group on the behavioral score and cerebral infarction volume percentage of tMCAO model rats are shown in Table 1.

[0065] Table 1 Behavioral scores and cerebral infarction volume percentages of rats after administration of decoctions in each experimental group

[0066] Experimental group Behavioral score Percentage of cerebral infarction volume (%) Model group 2.9±0.7 48.3±6.8 Example 1 group 1.6±0.5 21.6±3.5 Example 2 group 1.9±0.6 28.5±4.2 Example 3 group 2.0±0.7 31.2±4.9 Example 4 group 2.1±0.6 32.5±5.1 Control Example 1 group 2.3±0.9 38.4±5.5 Control Example 2 group 2.4±0.7 37.1±5.8

[0067] As can be seen from Table 1 above, the cerebral infarction volume percentage and behavioral score of the rats in the model group were 48.3 ± 6.8% and 2.9 ± 0.7 respectively, indicating that the model animals had obvious cerebral ischemia injury. Compared with the model group and Comparative Examples 1 - 2 groups, after the decoctions of Examples 1 - 4 of the present invention were given to the model rats, the cerebral infarction volume percentage and behavioral score of the rats decreased significantly (with statistical differences), indicating that the traditional Chinese medicine decoction of the present invention has a significant effect on treating ischemic stroke model rats, suggesting that the decoction of the present invention can be used clinically to treat stroke, especially acute ischemic stroke. Surprisingly, after the rats in the model group orally administered the decoction of Example 1 of the present invention, the cerebral infarction volume percentage and behavioral score of the rats decreased most significantly, producing an unexpectedly excellent therapeutic effect.

[0068] 2.2. Results of determination of the contents of inflammatory factors TNF-α and IL-β in rat brains

[0069] The effects of the traditional Chinese medicine decoctions of each experimental group on the inflammatory factors in the brains of tMCAO model rats are shown in Table 2 below.

[0070] Table 2 Contents of TNF-α and IL-β in the brains of rats after administration of the decoctions of each experimental group

[0071] Experimental group TNF-α (pg / μgprot) IL-β (pg / μgprot) Model group 29.36±2.75 643.8±38.6 Example 1 group 8.26±1.21 396.4±23.7 Example 2 group 14.57±1.46 451.2±26.3 Example 3 group 16.24±1.72 469.4±27.9 Example 4 group 17.93±1.68 482.5±25.4 Control Example 1 group 20.59±1.94 538.2±29.1 Control Example 2 group 22.74±2.05 557.1±32.5

[0072] As shown in Table 2 above, compared with the model group and Comparative Examples 1-2 groups, the contents of TNF-α and IL-1β in the damaged side of the brain tissue in the groups of Examples 1-4 of the present invention can be significantly reduced (with statistical differences). The above test results suggest that the traditional Chinese medicine decoction of the present invention (especially the traditional Chinese medicine decoction of Example 1) may be related to its inhibition of the expression of inflammatory factors in the treatment and improvement of ischemic reperfusion injury in the rat model.

[0073] Although the specific embodiments of the present invention have been described, those skilled in the art should recognize that various changes and modifications can be made to the present invention without departing from the scope or spirit of the present invention. Therefore, the present invention is intended to cover all such changes and modifications that fall within the scope of the appended claims and their equivalents.

Claims

1. A traditional Chinese medicine composition for treating stroke, characterized in that, The traditional Chinese medicine composition is prepared from 20 parts by weight of Poria cocos, 15 parts by weight of Uncaria rhynchophylla, 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Citrus reticulata Blanco, 10 parts by weight of Gastrodia elata Blume, 10 parts by weight of Ligusticum chuanxiong Hort., 9 parts by weight of Pinellia ternata (Thunb.) Breit., 6 parts by weight of Arisaema cum Bile and 6 parts by weight of Carthamus tinctorius L.

2. The traditional Chinese medicine composition according to claim 1, wherein The traditional Chinese medicine composition further comprises a pharmaceutically acceptable excipient.

3. The traditional Chinese medicine composition according to claim 2, wherein The traditional Chinese medicine composition is an oral preparation.

4. The traditional Chinese medicine composition according to claim 3, wherein The dosage form of the traditional Chinese medicine composition is tablet, capsule, granule, tincture or decoction.

5. The preparation method of the traditional Chinese medicine composition according to any one of claims 1-4, characterized in that, The preparation method comprises the following steps: taking the prescribed amounts of Poria cocos, Uncaria rhynchophylla, Salvia miltiorrhiza, Citrus reticulata Blanco, Gastrodia elata Blume, Ligusticum chuanxiong Hort., Pinellia ternata (Thunb.) Breit., Arisaema cum Bile and Carthamus tinctorius L., adding water 3 to 5 times the total weight of the Chinese medicinal materials, boiling over high heat and then decocting over low heat for 30 minutes, filtering, and concentrating the Chinese medicine filtrate to the weight of the initially added Chinese medicinal materials, and optionally adding a pharmaceutically acceptable excipient to obtain the product.

6. Use of the traditional Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a medicament for treating stroke.