Subpackaging equipment for stem cell culture medium

Abstract

The invention relates to sub-packaging equipment for a stem cell culture medium in the technical field of stem cell culture. The sub-packaging equipment comprises a sub-packaging barrel; a bearing sleeve is installed on the inner wall of the bottom of the subpackaging barrel, the rotating ring is installed on the outer wall of the bearing sleeve, and a rotating assembly is arranged on one side of the rotating ring; the number of the special culture medium trays is multiple, and the multiple special culture medium trays are sequentially stacked on the upper portion of the rotating ring. It is guaranteed that the interior of the split charging barrel is in a sterile state all the time, external bacteria air is prevented from entering the split charging barrel to affect the culture effect of a culture medium, the stem cell culture medium can be subjected to layered split charging treatment, different stem cell culture dishes in different layers can be rapidly taken out in a sealed environment, and the quality of the stem cell culture dishes is improved. And the performance of rapidly taking out and putting in the culture medium is achieved, and the operation mode of taking out and putting in the culture medium one by one is avoided, so that the whole subpackaging equipment has the effect of being convenient to use.

Description

Technical Field

[0001] The invention relates to the technical field of stem cell culture, and in particular discloses a packaging device for stem cell culture medium. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. Stem cell culture medium is a special culture environment required in the culture process of stem cells to achieve the adhesion or proliferation effect of stem cells. When the stem cell culture medium is packaged and stored, it needs to be stored in a sterile environment. After a period of storage, stem cells need to be taken out and observed regularly. However, when observing, the packaging equipment needs to be opened and the culture medium needs to be taken out, so that the sterile gas in the air will enter the packaging equipment, affecting the culture effect of the culture medium.

[0003] In order to solve the problem of sterile gases in the air entering the filling equipment and affecting the culture, a certain stem cell culture medium filling equipment on the market adopts a closed design for removing the culture medium, which has a certain market share.

[0004] After searching, the invention patent with the announcement number CN116588494B discloses a filling device for stem cell culture medium, including a filling barrel and a culture tube, the bottom end of the inner wall of the filling barrel is provided with a storage mechanism for storing the culture tube, the storage mechanism is provided with an adjustment mechanism for driving the circular motion of the culture tube, one side of the storage mechanism is provided with a material passage slot for the culture tube to slide through; a material guide port is provided on the filling barrel corresponding to the material passage slot, the inner wall of the filling barrel is provided with a sealing mechanism that slides and seals at the material guide port; a push-pull mechanism for driving the culture tube to move is provided at the axis of the bottom end of the inner wall of the filling barrel. Through the setting of the adjustment mechanism, the push-pull mechanism and the sealing mechanism, the culture tube can be taken out in a closed environment, so as to prevent the stem cell culture medium in other culture tubes stored in the filling barrel from being affected during the process of taking out the culture tube, and to prevent the temperature change from affecting the culture effect of the culture medium, thereby improving the use efficiency.

[0005] Based on the above search and in combination with the prior art, it is found that when the existing filling equipment is performing the removal operation for observation of the culture medium, it is necessary to open and close the sealing door and the sealing baffle in sequence. However, when the sealing door is opened, the material guide box is easy to contact with the outside air, so that the outside sterile air enters the inside of the material guide box and then enters the inside of the filling equipment, which still affects the culture effect of the culture medium. Moreover, the prior art adopts the operation mode of taking out and putting in the culture medium one by one, which is not easy to use. When loading and taking all the culture media, the operation procedure is relatively complicated, which increases the operation time of the entire filling equipment and the use intensity of the operators. Therefore, a filling equipment for stem cell culture medium is proposed to improve the above problems. Summary of the invention

[0006] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is that when the sealing door is opened, the material guide box is easy to contact with the outside air, so that the outside sterile air enters into the material guide box and then enters into the filling equipment, which still affects the culture effect of the culture medium.

[0007] In order to solve the above problems, the present invention provides a packaging device for stem cell culture medium, comprising:

[0008] Packing barrels;

[0009] A rotating circle, wherein a bearing sleeve is installed on the inner wall of the bottom of the dispensing barrel, and the rotating circle is installed on the outer wall of the bearing sleeve, and a rotating component is arranged on one side of the rotating circle;

[0010] A special culture medium tray, wherein the special culture medium tray is designed to be multiple, and the multiple special culture medium trays are sequentially stacked on the rotating circle, and the top corner of the special culture medium tray is provided with placement grooves that are equidistantly distributed in an annular shape, and one end of the placement groove is provided with a docking port that communicates with the inner wall of the special culture medium tray, and culture dishes are placed inside the placement grooves, and clamping components for positioning the culture dishes are provided on both sides of the inner wall of the placement grooves;

[0011] An outer material guide box and an inner material guide box, one side of the dispensing barrel is provided with material guide ports which are equidistant and vertically distributed, the outer material guide box and the inner material guide box are respectively fixed on both sides of the material guide ports, the number of the material guide ports is the same as the number of the special culture medium trays, and the bottom of the material guide port, the bottom inner wall of the outer material guide box, and the bottom inner wall of the inner material guide box are flush with the bottom of the placement groove, and sterilization components are arranged on both sides of the outer material guide box;

[0012] A fixing frame, the fixing frame is installed on the bottom inner wall of the dispensing barrel, and a lifting assembly is provided on the fixing frame, and an ejection assembly for ejecting the culture dish is provided on the lifting assembly;

[0013] The bottom of the outer material guide box and the bottom of the inner material guide box are both provided with sealing openings, and the inner walls of the sealing openings are plugged with sealing baffles, one row of sealing baffles is attached to the inner wall of the outer material guide box, and sealing grooves are provided around the sealing baffles, and sealing strips are fixed to the inner walls of the sealing grooves, and reciprocating lifting mechanisms are arranged on both sides of the two rows of sealing baffles, and the reciprocating lifting mechanism includes a mounting bracket installed at the bottom of one side of the filling barrel, one side of the mounting bracket and one side of the filling barrel are both provided with mounting holes, the inner walls of the mounting holes are rotatably connected with a driving shaft through a sealing bearing, and first gears are installed on both sides of the outer wall of the driving shaft, a connecting frame is fixed to the bottom of one side of the two rows of sealing baffles, and a rack plate is fixed to the bottom of one side of one of the connecting frames, and an L-shaped rack is fixed to the bottom of one side of the other connecting frame, the L-shaped rack is meshed with one side of one of the first gears, and the rack plate is meshed with the other side of the other first gear, and a reciprocating electric push rod for adjusting the height of the L-shaped rack is fixedly installed on one side of the top of the mounting bracket.

[0014] As a further supplement to the present application, the sterilization component includes light cloth tubes fixedly installed on both sides of the outer material guide box, and the inner walls of the light cloth tubes are connected to the interior of the outer material guide box, and ultraviolet sterilization lamps are fixedly installed at the ends of the light cloth tubes.

[0015] As a further supplement to the present application, the top of the special culture medium tray is provided with second positioning holes distributed at equal distances, and the bottom of the special culture medium tray is fixed with positioning columns distributed at equal distances, the bottom of the positioning columns are inserted into the first positioning holes and the second positioning holes, and second handle racks are fixed on both sides of the top of the special culture medium tray.

[0016] As a further supplement to the present application, the clamping assembly includes connecting grooves opened on both sides of the inner wall of the placement groove, and the inner walls of the connecting grooves are rotatably connected to an arc-shaped clamping plate attached to the outer wall of the culture dish through a pin shaft, and a spring is fixed to one side of the arc-shaped clamping plate and the inner wall of the connecting groove.

[0017] As a further supplement to the present application, the rotating assembly includes tooth grooves equidistantly arranged at the bottom of the outer wall of the rotating circle, and a second gear is meshed on one side of the tooth groove, and a stepper motor for driving the second gear to rotate is installed on the bottom inner wall of the filling barrel.

[0018] As a further supplement to the present application, the lifting assembly includes a threaded rod rotatably arranged between one end of the top of the fixing frame and the inner wall of the bottom of the filling barrel, and a first driven gear is installed at the bottom of the outer wall of the threaded rod, a first driving gear is meshed on one side of the first driven gear, and a first forward and reverse motor for driving the first driving gear to rotate is installed on the inner wall of the bottom of the filling barrel, the ejection assembly includes a U-shaped seat screwed on the threaded rod, and a guide rod is fixed between the other end of the top of the fixing frame and the inner wall of the bottom of the filling barrel, and a guide hole for the guide rod to pass through is opened on the U-shaped seat.

[0019] As a further supplement to the present application, a guide opening is provided at one end of the U-shaped seat, and a rack seat is slidably provided on the inner wall of the guide opening, an ejector head is installed at one end of the rack seat, and the position of the ejector head corresponds to the position of the docking port, a second driving gear is rotatably connected to one side of the inner wall of the guide opening, and the second driving gear is meshed with the rack seat, and a second forward and reverse motor for driving the second driving gear to rotate is installed on the top side of the U-shaped seat.

[0020] As a further supplement to the present application, an infrared position receiver is installed at the other end of the rack seat, and infrared position transmitters distributed equidistantly are installed on one side of the fixed frame, and the positions of the infrared position transmitters, the infrared position receivers and the docking interface correspond to each other.

[0021] As a further supplement to the present application, an installation bottom groove is opened at the bottom of the filling barrel, and a controller is sealed and installed on the inner wall of the installation bottom groove. The controller is electrically connected to the reciprocating electric push rod, the first forward and reverse motor, the second forward and reverse motor, the stepper motor, the infrared position transmitter and the infrared position receiver. A sterilizer is fixedly installed on one side of the bottom of the filling barrel, and the controller is electrically signal-connected to the sterilizer, the controller, the infrared position transmitter, the infrared position receiver and the first forward and reverse motor.

[0022] As a further supplement to the present application, a sealing cover is screwed on the top of the dispensing barrel, and the sealing cover and the dispensing barrel are in a sealed state. A first handle frame is fixed on the top of the sealing cover, and support pads are installed at the four corners of the bottom of the dispensing barrel.

[0023] In summary, after adopting the above structure, the present invention has the following advantages compared with the prior art:

[0024] 1. The above-mentioned sub-packaging barrel, outer material guide box, sealing baffle, reciprocating lifting mechanism, sterilization component, special culture medium tray, culture dish, inner material guide box and ejection component are used. The culture dish is pushed into the outer material guide box by the pushing action of the ejection component, and the two rows of sealing baffles are quickly driven by the reciprocating lifting mechanism to make the inner material guide box quickly closed and the outer material guide box quickly opened, effectively preventing the external sterile air from entering the interior of the sub-packaging barrel along the material guide port, and realizing the performance of quickly taking out the culture dish in a sealed environment. The internal air of the outer material guide box, the material guide port and the inner material guide box can be sterilized under the action of the sterilization component. When the two rows of sealing baffles are reset, it can ensure that the interior of the sub-packaging barrel is always in a sterile state, and prevent the external sterile air from entering the interior of the sub-packaging barrel to affect the culture effect of the culture medium;

[0025] 2. By using the above-mentioned dispensing barrel, sealing cover, special culture medium tray, rotating circle, first positioning hole, positioning column, second positioning hole and second handle frame, multiple stem cell culture dishes can be placed on the special culture medium tray, so that the special culture medium tray can be stacked inside the dispensing barrel, on the one hand, the layered dispensing treatment of the stem cell culture medium is realized, and a certain batch of stem cell culture medium is effectively dispensed, and on the other hand, the entire special culture medium tray can be directly taken out or put in when taking out and putting in the culture medium, so as to achieve the performance of fast taking out and putting in the culture medium, avoid the operation mode of taking out and putting in the culture medium one by one in the prior art, so that the whole dispensing equipment has the effect of being easy to use, and the operation procedure of taking out and putting in is simpler, which reduces the operation time of the whole dispensing equipment and reduces the use intensity of the operators;

[0026] 3. By using the above-mentioned rotating assembly, lifting assembly, ejection assembly, outer material guide box, inner material guide box, infrared position transmitter, infrared position receiver and controller, when observing different stem cell culture dishes in different layers, the lifting assembly can be used to adjust the height of the ejection assembly, and the infrared position transmitter, infrared position receiver and controller are used to position the height adjustment of the ejection assembly, so that the ejection head in the ejection assembly corresponds to the stem cell culture dishes in different layers, and the rotating assembly drives the rotating and superimposed special culture medium trays to rotate, so that the ejection head corresponds to different stem cell culture dishes in the same layer, so that different stem cell culture dishes in different layers can be ejected, which is convenient for observing different culture dishes in different layers and improves the use effect of the entire equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the three-dimensional structure of a packaging device for stem cell culture medium;

[0028] Figure 2 A three-dimensional cross-sectional view of a packaging device for stem cell culture medium;

[0029] Figure 3 A schematic diagram of a filling barrel and a rotating circle structure of a filling device for stem cell culture medium;

[0030] Figure 4 A schematic diagram of a filling barrel and a material guide port structure of a filling device for stem cell culture medium;

[0031] Figure 5 A schematic diagram of the structure of a lifting component of a filling device for stem cell culture medium;

[0032] Figure 6 It is a schematic diagram of the structure of a guide port and a second driving gear of a stem cell culture medium dispensing device;

[0033] Figure 7 A top view of a filling barrel of a filling device for stem cell culture medium;

[0034] Figure 8 A schematic diagram of the structure of a special culture medium tray for a stem cell culture medium dispensing device;

[0035] Fig. 9 A schematic diagram of an arc-shaped clamp and spring structure of a packaging device for stem cell culture medium;

[0036] Fig.10 It is a schematic diagram of the structure of an outer material guide box and an inner material guide box of a packaging device for stem cell culture medium;

[0037] Fig.11 A schematic diagram of the sealing port and ultraviolet sterilization lamp structure of a packaging device for stem cell culture medium;

[0038] Fig.12 The present invention is a schematic diagram of the structure of a reciprocating lifting mechanism of a filling device for stem cell culture medium.

[0039] Description of the numbers in the figure:

[0040] 1. Packing barrel; 2. Sealing cover; 3. First handle frame; 4. Outer guide box; 5. Sealing baffle; 6. Reciprocating lifting mechanism; 601. Mounting support frame; 602. Reciprocating electric push rod; 603. Mounting hole; 604. Driving shaft; 605. First gear; 606. Connecting frame; 607. Rack plate; 608. L-shaped rack; 7. Lighting tube; 8. Turning circle; 9. Special culture medium tray; 10. Placement slot; 11. Culture dish; 12. Fixing frame; 13. Inner guide box; 14. Lifting assembly; 1401. First forward and reverse motor; 1402. First driving gear; 1403. First driven gear; 1404. Threaded rod; 1405. Guide rod; 15. First positioning hole; 16, ejector assembly; 1601, U-shaped seat; 1602, second forward and reverse motor; 1603, ejector head; 1604, rack seat; 1605, guide port; 1606, second driving gear; 17, rotating assembly; 1701, stepping motor; 1702, second gear; 1703, tooth groove; 18, bearing sleeve; 19, controller; 20, guide port; 21, infrared position transmitter; 22, infrared position receiver; 23, second handle frame; 24, positioning column; 25, second positioning hole; 26, docking port; 27, connecting groove; 28, arc clamp; 29, spring; 30, ultraviolet sterilization lamp; 31, sealing strip; 32, sealing port; 33, sterilizer. DETAILED DESCRIPTION

[0041] The implementation methods of the present application are described in detail below with reference to the accompanying drawings.

[0042] See also Figure 1-Figure 12 The present invention provides a packaging device for stem cell culture medium, comprising:

[0043] A filling barrel 1, a sealing cover 2 is screwed on the top of the filling barrel 1, and the sealing cover 2 and the filling barrel 1 are in a sealed state, a first handle frame 3 is fixed on the top of the sealing cover 2, and support pads are installed at the four corners of the bottom of the filling barrel 1;

[0044] The inner wall of the bottom of the dispensing barrel 1 is provided with a bearing sleeve 18, and the outer wall of the bearing sleeve 18 is provided with a rotating assembly 17 on one side of the rotating ring 8. The rotating assembly 17 includes a tooth groove 1703 equidistantly provided at the bottom of the outer wall of the rotating ring 8, and a second gear 1702 is meshed on one side of the tooth groove 1703. A stepping motor 1701 for driving the second gear 1702 to rotate is installed on the inner wall of the bottom of the dispensing barrel 1;

[0045] A special culture medium tray 9 is designed to be multiple, and multiple special culture medium trays 9 are stacked on the top of the rotating circle 8, and the top corner of the special culture medium tray 9 is provided with equidistant annular placement grooves 10, one end of the placement groove 10 is provided with a docking port 26 that communicates with the inner wall of the special culture medium tray 9, and culture dishes 11 are placed inside the placement grooves 10, and both sides of the inner wall of the placement grooves 10 are provided with clamping components for positioning the culture dishes 11, and the clamping components include openings The connecting grooves 27 are arranged on both sides of the inner wall of the placement groove 10, and the inner walls of the connecting grooves 27 are rotatably connected with arc-shaped clamping plates 28 that are attached to the outer wall of the culture dish 11 through pins. A spring 29 is fixed to one side of the arc-shaped clamping plate 28 and the inner wall of the connecting groove 27. The tensioning performance of the spring 29 in the clamping assembly is used to fit the arc-shaped clamping plate 28 to the outer wall of the culture dish 11, so that the culture dish 11 is fixed in the placement groove 10 of the special culture medium tray 9, thereby ensuring the stability of the culture dish 11 on the special culture medium tray 9;

[0046] The outer material guide box 4 and the inner material guide box 13, one side of the filling barrel 1 is provided with material guide ports 20 which are equidistant and vertically distributed, the outer material guide box 4 and the inner material guide box 13 are respectively fixed on both sides of the material guide port 20, the number of the material guide ports 20 is the same as the number of the special culture medium tray 9, and the bottom of the material guide port 20, the bottom inner wall of the outer material guide box 4, and the bottom inner wall of the inner material guide box 13 are flush with the bottom of the placement groove 10, and sterilization components are arranged on both sides of the outer material guide box 4, the sterilization components include light cloth tubes 7 fixedly installed on both sides of the outer material guide box 4, and the inner walls of the light cloth tubes 7 are communicated with the interior of the outer material guide box 4, and the ends of the light cloth tubes 7 are fixedly installed with ultraviolet sterilization lamps 30, which can sterilize the internal air of the outer material guide box 4, the material guide port 20 and the inner material guide box 13 under the action of the sterilization component, so as to ensure that the interior of the filling barrel 1 is always in a sterile state, and prevent the external sterile air from entering the interior of the filling barrel 1 and affecting the culture effect of the culture medium;

[0047] A fixing frame 12, the fixing frame 12 is mounted on the bottom inner wall of the dispensing barrel 1, and a lifting assembly 14 is provided on the fixing frame 12, and an ejection assembly 16 for ejecting the culture dish 11 is provided on the lifting assembly 14;

[0048] The bottom of the outer material guide box 4 and the bottom of the inner material guide box 13 are both provided with sealing openings 32, and the inner walls of the sealing openings 32 are both plugged with sealing baffles 5, one row of sealing baffles 5 is attached to the inner wall of the outer material guide box 4, and sealing grooves are provided around the sealing baffles 5, and the inner walls of the sealing grooves are fixed with sealing strips 31, and reciprocating lifting mechanisms 6 are provided on both sides of the two rows of sealing baffles 5, and the reciprocating lifting mechanism 6 includes a mounting bracket 601 installed at the bottom of one side of the filling barrel 1, and one side of the mounting bracket 601 and one side of the filling barrel 1 are both provided with mounting holes 603, and the inner wall of the mounting hole 603 is rotatably connected to a driving shaft 604 through a sealing bearing, and the outer walls of the driving shaft 604 are both installed with first gears 605, and the bottom of one side of the two rows of sealing baffles 5 is fixed. There is a connecting frame 606, and a rack plate 607 is fixed to the bottom of one side of one of the connecting frames 606, and an L-shaped rack 608 is fixed to the bottom of one side of the other connecting frame 606. The L-shaped rack 608 is meshed on one side of one of the first gears 605, and the rack plate 607 is meshed on the other side of the other first gear 605. A reciprocating electric push rod 602 for adjusting the height of the L-shaped rack 608 is fixedly installed on one side of the top of the mounting bracket 601. The reciprocating lifting mechanism 6 is used to quickly drive the two rows of sealing baffles 5 to move, so that the inner material guide box 13 is quickly closed and the outer material guide box 4 is quickly opened, which effectively prevents the external bacteria-carrying air from entering the interior of the filling barrel 1 along the material guide port 20, and realizes the performance of quickly taking out the culture dish 11 in a sealed environment.

[0049] Specifically, the top of the special culture medium tray 9 is provided with second positioning holes 25 distributed at equal distances, and the bottom of the special culture medium tray 9 is fixed with positioning columns 24 distributed at equal distances, the bottom of the positioning columns 24 is inserted into the first positioning holes 15 and the second positioning holes 25, and second handle frames 23 are fixed on both sides of the top of the special culture medium tray 9. The special culture medium tray 9 is used to carry out the packaging of multiple stem cell culture media, and the special culture medium trays 9 can be easily stacked under the clamping action of the positioning columns 24, the first positioning holes 15 and the second positioning holes 25, so as to effectively package a certain batch of stem cell culture media, and the entire special culture medium tray 9 can be directly taken out or put in when taking out and putting in the culture medium, thereby realizing the performance of quickly taking out and putting in the culture medium.

[0050] Specifically, the lifting assembly 14 includes a threaded rod 1404 rotatably arranged between one end of the top of the fixing frame 12 and the inner wall of the bottom of the filling barrel 1, and a first driven gear 1403 is installed at the bottom of the outer wall of the threaded rod 1404, and a first driving gear 1402 is meshed on one side of the first driven gear 1403. A first forward and reverse motor 1401 for driving the first driving gear 1402 to rotate is installed on the inner wall of the bottom of the filling barrel 1. The ejection assembly 16 includes a U-shaped seat 1601 screwed on the threaded rod 1404, and a guide rod 1405 is fixed between the other end of the top of the fixing frame 12 and the inner wall of the bottom of the filling barrel 1, and a guide hole for the guide rod 1405 to pass through is opened on the U-shaped seat 1601, a guide port 1605 is opened at one end of the U-shaped seat 1601, and a rack seat 16 is slidably arranged on the inner wall of the guide port 1605. 04, an ejector head 1603 is installed at one end of the rack seat 1604, and the position of the ejector head 1603 corresponds to the position of the docking port 26. A second driving gear 1606 is rotatably connected to one side of the inner wall of the guide port 1605, and the second driving gear 1606 is meshed with the rack seat 1604. A second forward and reverse motor 1602 for driving the second driving gear 1606 to rotate is installed on one side of the top of the U-shaped seat 1601. The lifting component 14 is used to adjust the height of the ejector component 16, and the rotating circle 8 and the superimposed special culture medium tray 9 are driven to rotate through the rotating component 17, so that the ejector head 1603 corresponds to different stem cell culture dishes 11 of different layers, so that different stem cell culture dishes 11 of different layers can be ejected, which is convenient for observing different culture dishes 11 of different layers and improving the use effect of the entire equipment.

[0051] Specifically, an infrared position receiver 22 is installed at the other end of the rack seat 1604, and infrared position transmitters 21 are installed at equal distances on one side of the fixed frame 12. The position of the infrared position transmitter 21, the position of the infrared position receiver 22 and the position of the docking port 26 correspond to each other. A mounting bottom groove is provided at the bottom of the dispensing barrel 1, and a controller 19 is sealed and installed on the inner wall of the mounting bottom groove. A sterilizer 33 is fixedly installed on one side of the bottom of the dispensing barrel 1. The controller 19 is connected to the sterilizer 33, the reciprocating electric push rod 602, the first forward and reverse motor 1401, and the second forward and reverse motor 16 02. The stepper motor 1701, the infrared position transmitter 21 and the infrared position receiver 22 are electrically connected, and the controller 19, the infrared position transmitter 21, the infrared position receiver 22 and the first forward and reverse motor 1401 are electrically connected. The height adjustment of the ejection assembly 16 is positioned through the cooperation of the infrared position transmitter 21, the infrared position receiver 22 and the controller 19, so that the ejection head 1603 in the ejection assembly 16 can correspond to the stem cell culture dishes 11 in different layers, and the sterilization operation of the sterilizer 33 is used to make the inside of the filling barrel 1 in a sterile state.

[0052] In summary, the working principle of the present invention is as follows: when in use, the user sequentially packs the culture dishes 11 for stem cell culture medium on different special culture medium trays 9, and uses the tensioning performance of the spring 29 in the clamping assembly to fit the arc-shaped clamping plate 28 to the outer wall of the culture dish 11, so that the culture dish 11 is fixed in the placement groove 10 of the special culture medium tray 9, and then stacks a plurality of special culture medium trays 9 inside the filling barrel 1, so that the positioning column 24 can be inserted into the first positioning hole 15 and the second positioning hole 25, so as to realize the layered filling operation of the stem cell culture medium, and can also directly take out or put in the entire special culture medium tray 9 when taking out and putting in the culture medium, and then closes the sealing cover 2 on the filling barrel 1, and then the sealing cover 2 is used. The sterilization operation of the sterilizer 33 makes the inside of the dispensing barrel 1 in a sterile state; when the user observes different stem cell culture dishes 11 in different layers, the first forward and reverse motor 1401 in the lifting component 14 is used to drive the first driving gear 1402, the first driven gear 1403 and the threaded rod 1404 to rotate, and the U-shaped seat 1601 in the ejection component 16 is adjusted to rise and fall, and the infrared position transmitter 21, the infrared position receiver 22 and the controller 19 are used to position the height adjustment of the ejection component 16, so that the ejection head 1603 in the ejection component 16 corresponds to the stem cell culture dishes 11 in different layers, and then the user drives the stepping motor 1701 in the rotating component 17 to rotate. The second gear 1702 rotates, and the meshing action of the second gear 1702 and the tooth groove 1703 drives the rotating circle 8 and the superimposed special culture medium tray 9 to rotate, so that the ejection head 1603 corresponds to different stem cell culture dishes 11 at the same level, and then the second forward and reverse motor 1602 in the ejection assembly 16 drives the second driving gear 1606 to rotate, and the meshing action of the second driving gear 1606 and the rack seat 1604 is used to move the ejection head 1603 into the placement groove 10, so that the culture dish 11 is pushed into the inner part of the outer guide box 4, and then the ejection assembly 16 is reset, and the reciprocating electric push rod 602 in the reciprocating lifting mechanism 6 is used to adjust the height of the L-shaped rack 608, driving the first gear 605 and the driving shaft 604 rotate, so that the rack plate 607 and the L-shaped rack 608 form a reverse movement, which quickly drives the two rows of sealing baffles 5 to move, so that the inner material guide box 13 is quickly closed and the outer material guide box 4 is quickly opened, effectively preventing the external sterile air from entering the interior of the filling barrel 1 along the material guide port 20, and realizing the performance of quickly taking out the culture dish 11 in a sealed environment, so that different stem cell culture dishes 11 in different layers can be taken out, which is convenient for observation and can sterilize the internal air of the outer material guide box 4, the material guide port 20 and the inner material guide box 13 under the action of the ultraviolet sterilization lamp 30 in the sterilization component. When the two rows of sealing baffles 5 are reset, it can be ensured that the interior of the filling barrel 1 is always in a sterile state.

[0053] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A packaging device for stem cell culture medium, characterized in that: include: Dispensing barrel (1); A rotating circle (8), a bearing sleeve (18) is installed on the inner wall of the bottom of the dispensing barrel (1), and the rotating circle (8) is installed on the outer wall of the bearing sleeve (18), and a rotating component (17) is arranged on one side of the rotating circle (8); A special culture medium tray (9), wherein the special culture medium tray (9) is designed to be multiple, and the multiple special culture medium trays (9) are sequentially stacked on the rotating circle (8), and the top corner of the special culture medium tray (9) is provided with equidistant and annular placement grooves (10), one end of the placement groove (10) is provided with a docking port (26) that communicates with the inner wall of the special culture medium tray (9), and culture dishes (11) are placed inside the placement grooves (10), and clamping components for positioning the culture dishes (11) are provided on both sides of the inner wall of the placement grooves (10); An outer material guide box (4) and an inner material guide box (13), one side of the dispensing barrel (1) is provided with material guide openings (20) which are equidistant and vertically distributed, the outer material guide box (4) and the inner material guide box (13) are respectively fixed on both sides of the material guide opening (20), the number of the material guide openings (20) is the same as the number of the special culture medium tray (9), and the bottom of the material guide opening (20), the bottom inner wall of the outer material guide box (4), and the bottom inner wall of the inner material guide box (13) are flush with the bottom of the placement groove (10), and sterilization components are arranged on both sides of the outer material guide box (4); A fixing frame (12), the fixing frame (12) being mounted on the bottom inner wall of the dispensing barrel (1), and a lifting assembly (14) being arranged on the fixing frame (12), and an ejection assembly (16) for ejecting the culture dish (11) being arranged on the lifting assembly (14); The bottom of the outer material guide box (4) and the bottom of the inner material guide box (13) are both provided with sealing openings (32), and the inner walls of the sealing openings (32) are both plugged with sealing baffles (5), wherein one row of sealing baffles (5) is attached to the inner wall of the outer material guide box (4), and sealing grooves are provided around the sealing baffles (5), and sealing strips (31) are fixed to the inner walls of the sealing grooves, and reciprocating lifting mechanisms (6) are provided on both sides of the two rows of sealing baffles (5), and the reciprocating lifting mechanisms (6) include a mounting bracket (601) mounted on the bottom of one side of the dispensing barrel (1), and mounting holes (603) are provided on one side of the mounting bracket (601) and one side of the dispensing barrel (1), and the inner wall of the mounting hole (603) is sealed by a sealing The sealing bearing is rotatably connected to a driving shaft (604), and first gears (605) are installed on both sides of the outer wall of the driving shaft (604). A connecting frame (606) is fixed to the bottom of one side of the two rows of sealing baffles (5), and a rack plate (607) is fixed to the bottom of one side of one of the connecting frames (606), and an L-shaped rack (608) is fixed to the bottom of one side of the other connecting frame (606). The L-shaped rack (608) is meshed on one side of one of the first gears (605), and the rack plate (607) is meshed on the other side of the other first gear (605). A reciprocating electric push rod (602) for adjusting the height of the L-shaped rack (608) is fixed on one side of the top of the mounting bracket (601).

2. A stem cell culture medium packaging device according to claim 1, characterized in that: The sterilization component comprises light distribution tubes (7) fixedly mounted on both sides of the outer material guide box (4), wherein the inner walls of the light distribution tubes (7) are in communication with the interior of the outer material guide box (4), and ultraviolet sterilization lamps (30) are fixedly mounted on the ends of the light distribution tubes (7).

3. A stem cell culture medium packaging device according to claim 2, characterized in that: The top of the special culture medium tray (9) is provided with second positioning holes (25) distributed at equal distances, and the bottom of the special culture medium tray (9) is fixed with positioning columns (24) distributed at equal distances, the bottom of the positioning columns (24) is inserted into the inside of the first positioning hole (15) and the second positioning hole (25), and second handle frames (23) are fixed on both sides of the top of the special culture medium tray (9).

4. A stem cell culture medium packaging device according to claim 3, characterized in that: The clamping assembly comprises connecting grooves (27) provided on both sides of the inner wall of the placement groove (10), and the inner wall of the connecting groove (27) is rotatably connected to an arc-shaped clamping plate (28) affixed to the outer wall of the culture dish (11) via a pin, and a spring (29) is fixed to one side of the arc-shaped clamping plate (28) and the inner wall of the connecting groove (27).

5. A stem cell culture medium packaging device according to claim 4, characterized in that: The rotating assembly (17) includes tooth grooves (1703) arranged at equal distances on the bottom of the outer wall of the rotating circle (8), and a second gear (1702) is meshed on one side of the tooth groove (1703). A stepping motor (1701) for driving the second gear (1702) to rotate is installed on the bottom inner wall of the dispensing barrel (1).

6. A stem cell culture medium packaging device according to claim 5, characterized in that: The lifting assembly (14) comprises a threaded rod (1404) rotatably arranged between one end of the top of the fixing frame (12) and the inner wall of the bottom of the dispensing barrel (1), and a first driven gear (1403) is installed at the bottom of the outer wall of the threaded rod (1404), and a first driving gear (1402) is meshed on one side of the first driven gear (1403), and a first forward and reverse motor (1401) for driving the first driving gear (1402) to rotate is installed on the inner wall of the bottom of the dispensing barrel (1), and the ejection assembly (16) comprises a U-shaped seat (1601) screwed on the threaded rod (1404), and a guide rod (1405) is fixed between the other end of the top of the fixing frame (12) and the inner wall of the bottom of the dispensing barrel (1), and a guide hole for the guide rod (1405) to pass through is opened on the U-shaped seat (1601).

7. A stem cell culture medium packaging device according to claim 6, characterized in that: A guide opening (1605) is provided at one end of the U-shaped seat (1601), and a rack seat (1604) is slidably provided on the inner wall of the guide opening (1605), an ejector head (1603) is installed at one end of the rack seat (1604), and the position of the ejector head (1603) corresponds to the position of the docking port (26), a second driving gear (1606) is rotatably connected to one side of the inner wall of the guide opening (1605), and the second driving gear (1606) is meshed with the rack seat (1604), and a second forward and reverse motor (1602) for driving the second driving gear (1606) to rotate is installed on one side of the top of the U-shaped seat (1601).

8. The stem cell culture medium packaging device according to claim 7, characterized in that: An infrared position receiver (22) is installed at the other end of the rack seat (1604), and infrared position transmitters (21) distributed at equal distances are installed on one side of the fixed frame (12), and the positions of the infrared position transmitters (21), the infrared position receivers (22) and the docking interface (26) correspond to each other.

9. A stem cell culture medium packaging device according to claim 8, characterized in that: The bottom of the dispensing barrel (1) is provided with an installation bottom groove, and the inner wall of the installation bottom groove is sealed with a controller (19); a sterilizer (33) is fixedly installed on one side of the bottom of the dispensing barrel (1); the controller (19) is electrically connected to the sterilizer (33), a reciprocating electric push rod (602), a first forward and reverse motor (1401), a second forward and reverse motor (1602), a stepping motor (1701), an infrared position transmitter (21) and an infrared position receiver (22); and the controller (19), the infrared position transmitter (21), the infrared position receiver (22) and the first forward and reverse motor (1401) are electrically signal connected.

10. The stem cell culture medium packaging device according to claim 1, characterized in that: A sealing cover (2) is screwed onto the top of the dispensing barrel (1), and the sealing cover (2) and the dispensing barrel (1) are in a sealed state. A first handle frame (3) is fixed to the top of the sealing cover (2), and support pads are installed at the four corners of the bottom of the dispensing barrel (1).

Images