High-salt wastewater treatment method, halomonas wilandii and application thereof

Abstract

The present invention relates to a method for treating high-salt wastewater, halomonas wilandii and applications thereof. The method is applicable to the field of high-salt wastewater treatment. The technical scheme of the present invention is: a method for treating high-salt wastewater, characterized in that: halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011 is inoculated into high-salinity wastewater for wastewater treatment; the Halomonas wilandii ( Halomonas vreelandella Vilamensis ) FL011 is deposited in the China Center for Type Culture Collection, with the deposit number of CCTCC NO: M 20242136. The halomonas wilandii FL011 in the present invention has a high salinity tolerance and a relatively stable organic matter treatment efficiency. The test shows that the halomonas wilandii FL011 can effectively remove COD and Cl in high-salinity wastewater.

Description

Technical Field

[0001] The invention relates to a high-salt wastewater treatment method, halomonas wilandii and applications thereof, and is applicable to the field of high-salt wastewater treatment. Background Art

[0002] High-salt wastewater generated during petrochemical production and textile leather manufacturing is the most pressing problem to be solved in industrial production in recent years. High-salt wastewater refers to wastewater with a total dissolved solids mass fraction of ≥3.5%. The composition of industrial high-salt wastewater in different industries is quite different, making the treatment of industrial high-salt wastewater more difficult. Although biological methods are cheaper than chemical and physical methods, there are still problems such as low salinity tolerance and unstable organic matter treatment efficiency. The essential reason is that high salinity will cause an imbalance in the osmotic pressure inside and outside the cells, leading to water loss and ultimately killing the cells. Summary of the invention

[0003] The technical problem to be solved by the present invention is: in view of the above-mentioned existing problems, a method for treating high-salt wastewater, halomonas wilandii and applications thereof are provided.

[0004] The technical solution adopted by the present invention is: a method for treating high-salt wastewater, characterized in that: Halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011 was inoculated into high-salinity wastewater for wastewater treatment;

[0005] The Halomonas wilandii ( Halomonas vreelandella vilamensis )FL011 is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: M 20242136.

[0006] The Halomonas wilandii ( Halomonas vreelandella vilamensis ) The volume fraction of FL011 inoculation is one thousandth.

[0007] The wastewater treatment method is carried out in an anaerobic environment or an aerobic environment.

[0008] A species of Halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011, characterized in that: the Halomonas wilandii ( Halomonas vreelandella vilamensis )FL011 is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: M 20242136.

[0009] A kind of described Halomonas wilandii ( Halomonas vreelandella vilamensis )Application of FL011 in high-salinity wastewater treatment.

[0010] A kind of described Halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011 is used to remove COD and Cl in high-salinity wastewater. -Application in.

[0011] A kind of described Halomonas wilandii ( Halomonas vreelandella vilamensis ) Application of FL011 in COD removal.

[0012] A kind of described Halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011 is effective in removing Cl - Application in.

[0013] The beneficial effects of the present invention are: the halophilic mononas of Wielandia FL011 in the present invention has a high salinity tolerance and a relatively stable organic matter treatment efficiency. Experiments have shown that the halophilic mononas of Wielandia FL011 can effectively remove COD and Cl in high-salinity wastewater. - wait. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is the developmental evolution tree based on FL011.

[0015] Figure 2 These are the test results corresponding to the anaerobic (10 g / L sodium chloride) test in the examples.

[0016] Figure 3 These are the test results corresponding to the anaerobic (90 g / L sodium chloride) test in the examples.

[0017] Figure 4 These are the test results corresponding to the anaerobic (150 g / L sodium chloride) test in the examples.

[0018] Figure 5 These are the test results corresponding to aerobic conditions (10 g / L sodium chloride) in the examples.

[0019] Figure 6 These are the test results corresponding to aerobic conditions (90 g / L sodium chloride) in the examples.

[0020] Figure 7 These are the test results corresponding to aerobic conditions (150 g / L sodium chloride) in the examples. DETAILED DESCRIPTION

[0021] This embodiment is a method for treating high-salinity wastewater. The method comprises: Halomonas vreelandella vilamensis ) FL011 was inoculated into high-salinity wastewater for wastewater treatment. The COD and Cl in high-salinity wastewater were removed by using Halomonas wilandii FL011 - wait.

[0022] In this example, Halobacterium wilandii ( Halomonas vreelandella vilamensis)FL011, was deposited in the China Center for Type Culture Collection on September 30, 2024, with the address: Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: M 20242136.

[0023] (1) Screening of halophilic microorganisms

[0024] Sample source: Salt lake sediments in Jincheng City, Shanxi Province.

[0025] Screening medium: glucose 5 g / L, peptone 3 g / L, yeast extract 1 g / L, sodium chloride (30 g / L -150 g / L), pH 7.5-8.0.

[0026] Screening process: Prepare a series of screening medium with different sodium chloride concentrations. First, weigh 10 g of salt lake sediment and add it to 90 mL of screening medium with a sodium chloride concentration of 30 g / L. Incubate at 30°C and 100 r / min for 48 hours.

[0027] Then, 1 mL of the culture solution was taken out with a pipette and inoculated into a screening medium with a sodium chloride concentration of 60 g / L, and cultured at 30°C and 100 r / min for 48 h.

[0028] Then, 1 mL of culture solution was taken out with a pipette and inoculated into a screening medium with a sodium chloride concentration of 90 g / L, and cultured at 30°C and 100 r / min for 48 h.

[0029] Then, 1 mL of culture solution was taken out with a pipette and inoculated into a screening medium with a sodium chloride concentration of 120 g / L, and cultured at 30°C and 100 r / min for 48 h.

[0030] Then, use a pipette to draw 1 mL of the culture medium and inoculate it into a screening medium with a sodium chloride concentration of 150 g / L. Cultivate at 30°C and 100 r / min until the culture medium OD reaches 0. 600 Greater than 3.

[0031] Prepare a solid screening medium with a sodium chloride concentration of 150 g / L and prepare it into a plate. Use an inoculation loop to pick up the culture solution and streak it on the solid medium plate, place it at 30°C and culture it until a single colony grows out, which is named FL011.

[0032] (2) Identification of microorganisms

[0033] Pick a single colony of FL011 on the solid screening medium plate, pick DNA, and sequence its 16S rDNA. Its 16S rDNA sequence is:

[0034]

[0035] Figure 1 The phylogenetic tree based on FL011 showed that FL011 was a member of Halomonas wilandii ( Halomonas vreelandella vilamensis ).

[0036] (3) Application of Halomonas wilandii FL011 in the treatment of high-salinity wastewater

[0037] Simulated high-salt wastewater: Yangtze River water was used as the base water body, glucose was used as COD, ammonium chloride was used as the nitrogen source, and different concentrations of sodium chloride were added.

[0038] (3.1) FL011 simultaneously removes COD and Cl under anaerobic conditions - Experiment

[0039] In a 500mL triangular flask, prepare 350mL of simulated high-salinity wastewater. Set COD=3000 mg / L, ammonia nitrogen concentration of 15mg / L, and sodium chloride concentrations of 10 g / L, 30 g / L, 90 g / L, and 150 g / L, respectively. Inoculate FL011 bacterial solution with a volume fraction of 1‰, culture at 30℃, and sample once every 12 hours. Centrifuge the sample at 8000r / min for 10min, and take the supernatant to measure COD concentration and Cl - concentration.

[0040] (3.2) FL011 simultaneously removes COD and Cl under aerobic conditions - Experiment

[0041] In a 500mL triangular flask, prepare 200mL of simulated high-salinity wastewater. Set COD = 1000 mg / L, ammonia nitrogen concentration of 15mg / L, sodium chloride concentrations of 10 g / L, 30g / L, 90 g / L, and 150 g / L of wastewater, inoculate with FL011 bacterial solution with a volume fraction of 1‰, culture at 30℃, 150r / min, and sample once every 12h. Centrifuge the sample at 8000r / min for 10min, and take the supernatant to measure COD concentration and Cl - concentration.

[0042] (3.3) Experimental results

[0043] The experimental results under anaerobic conditions are as follows Figure 2 , Figure 3 , Figure 4 As shown in the figure, the highest COD removal rates of FL011 within 72 h were 96.04% (10 g / L NaCl), 95.56% (90 g / L NaCl) and 91.82% (150 g / L NaCl).

[0044] To Cl -The highest removal rates were 68.62% (10 g / L NaCl), 59.06% (90 g / L NaCl) and 53.17% (150 g / L NaCl).

[0045] The experimental results under aerobic conditions are as follows Figure 5 , Figure 6 , Figure 7 As shown in the figure, the highest COD removal rates of FL011 within 48 h were 91.02% (10 g / L NaCl), 91.34% (90 g / L NaCl) and 90.19% (150 g / L NaCl).

[0046] To Cl - The highest removal rates were 69.1% (10 g / L NaCl), 63.03% (90 g / L NaCl) and 57.59% (150 g / L NaCl).

Claims

1. A halomonas wilandii ( Halomonas vreelandella vilamensis ) FL011 is used to remove COD and Cl in high-salinity wastewater. - Applications in The Halomonas wilandii ( Halomonas vreelandella vilamensis )FL011 is deposited in China Center for Type Culture Collection with the deposit number CCTCC NO: M 20242136.

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