Sorting, filtering and sampling all-in-one machine for tumor cell clusters

Abstract

The invention belongs to the technical field of medical experiment devices, and particularly relates to a tumor cell cluster sorting, filtering and sampling all-in-one machine which comprises a shell, and a plurality of sampling holes formed at equal intervals are formed in the middle of the side wall of the shell; the filtering and sampling mechanism is arranged at the lower part in the shell; the variable-temperature storage mechanism is arranged at the upper part in the shell and corresponds to the filtering and sampling mechanism up and down; the pressure crushing mechanism is fixedly connected with the top end of the exterior of the shell and communicates with the interior of the shell; and the sampling mechanism is fixedly communicated with the pressure crushing mechanism through a connecting hose. According to the invention, the sorting speed and sorting effect of tumor cell clusters can be improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medical experimental devices, and in particular relates to an integrated machine for sorting, filtering and sampling tumor cell clusters. Background Art

[0002] The incidence and mortality of cancer have been increasing, and it has become one of the main causes of death in humans. The most common malignant tumors in clinical practice are mainly solid malignant tumors. There are many causes of solid malignant tumors. At present, the pathogenesis of most solid malignant tumors is still unclear. Traditional cancer treatment mainly relies on surgery, chemotherapy, and radiotherapy. Among them, chemotherapy, as the most commonly used systemic treatment, is widely used in all stages of cancer treatment and plays a pivotal role in cancer treatment. However, due to the large individual differences in chemotherapy, the efficacy of clinical chemotherapy is limited. If patients choose an inappropriate chemotherapy regimen, it may be counterproductive and difficult to benefit from it. The use of chemotherapy drug sensitivity detection technology to guide clinical individualized chemotherapy practice, help patients choose appropriate chemotherapy drugs, and reduce patients' chemotherapy adverse reactions and economic burden. There are many types of drug sensitivity detection technologies at present. Among them, collagen gel droplet embedding drug sensitivity detection technology (CD-DST) is a new in vitro drug sensitivity detection technology. It uses primary tumor cell groups in vitro three-dimensional culture for drug evaluation. It can effectively simulate the tumor microenvironment in the human body and obtain drug sensitivity detection data close to the human body. Therefore, it has broad clinical application prospects. In the process of tumor cell culture, tumor cell groups need to be sorted and filtered.

[0003] The utility model with application number CN201721033350.3 discloses a simple sorting and filtering device for tumor cell clusters (multicellular spheroids), including filter element 1, filter element 2 and connecting tube. The bottoms of the two filter elements are both filter screens; a funnel is provided at the top of the connecting tube, and a nozzle is provided on the outer wall, and the position of the nozzle is not less than 1 / 3 of the height above the bottom of the funnel. When in use, the sample liquid passes through filter element 1 and filter element 2 in turn, filter element 1 filters out large impurities such as tissue blocks, tumor cell clusters are intercepted by filter element 2, and single cells and fine impurity particles pass through the filter element 2 filter screen and flow into the connecting tube to be removed. The device can obtain cell clusters of uniform size required by one-time filtration, which can significantly reduce the loss of cell cluster quantity and damage to cell cluster activity caused by repeated operations; and can effectively remove single cells and fine impurities, avoid excessive fine impurity particles causing background signal interference to subsequent experiments, realize the overall standardization of the sorting process of cell clusters, and improve the controllability of the quality of the test process.

[0004] Based on the above content, the existing cell cluster sorting and filtration process generally adopts gravity filtration for sorting, which has a slow sorting speed and low sorting efficiency. It needs to be shaken regularly during the sorting process, which is cumbersome to use and operate, and does not meet the existing needs.

[0005] Therefore, it is necessary to design an integrated machine for sorting, filtering and sampling tumor cell clusters to solve the above problems. Summary of the invention

[0006] The purpose of the present invention is to provide an integrated machine for sorting, filtering and sampling tumor cell clusters to solve the above-mentioned problems and achieve the purpose of improving the sorting speed and sorting effect.

[0007] To achieve the above object, the present invention provides the following solution: a tumor cell cluster sorting, filtering and sampling integrated machine, comprising:

[0008] A shell, wherein a plurality of sampling holes arranged at equal intervals are opened in the middle of the side wall of the shell;

[0009] A filtering and sampling mechanism, the filtering and sampling mechanism is arranged at the lower part of the inner part of the housing;

[0010] A variable temperature storage mechanism, the variable temperature storage mechanism is arranged above the interior of the housing and corresponds to the filtering and sampling mechanism up and down;

[0011] A pressure crushing mechanism, the pressure crushing mechanism is fixedly connected to the top of the outer shell and communicated with the inside of the shell;

[0012] A sampling mechanism is fixedly connected to the pressure crushing mechanism through a connecting hose.

[0013] Preferably, the filtering sampling mechanism includes a rotating drive unit, the rotating drive unit is fixedly connected to the inner bottom wall of the shell, the output end of the rotating drive unit is coaxially fixedly connected to a bottom rotating disk, the bottom rotating disk is coaxially rotatably arranged inside the shell, the top of the bottom rotating disk is fixedly connected to a plurality of moving parts, the plurality of moving parts are arranged at equal intervals along the circumference of the bottom rotating disk, the top of the moving part is fixedly connected to a test tube clamping part, the center of the top of the bottom rotating disk is fixedly connected to the bottom end of a vertically arranged first telescopic rod, and the variable temperature preservation mechanism is fixedly connected to the top of the first telescopic rod.

[0014] Preferably, the moving part includes a second motor, the second motor is fixedly connected to the top of the bottom rotating disk, the second motor output shaft is fixedly connected to one end of a horizontally arranged screw, the other end of the screw is rotatably connected to a fixed seat, the bottom end of the fixed seat is fixedly connected to the top of the bottom rotating disk, the screw is threadedly connected to a sliding block, the sliding block is vertically arranged and the bottom end is in sliding contact with the top of the bottom rotating disk, and the test tube clamping part is fixedly connected to the top of the side wall of the sliding block away from the first telescopic rod.

[0015] Preferably, the test tube clamping portion includes two horizontal connecting rods, the two horizontal connecting rods are horizontally spaced apart and one end is fixedly connected to the side wall of the sliding block, the other ends of the two horizontal connecting rods are rotatably connected to the top end of a fixed cylinder via a rotating support ear, a test tube is detachably connected in the fixed cylinder, a sealing filter portion is detachably installed at the opening position of the test tube, the bottom end of the fixed cylinder is fixedly connected to the top ends of two spaced apart vertical connecting rods, a rotating shaft is fixedly connected between the bottom ends of the two vertical connecting rods, the rotating shaft is rotatably connected to one end of a second telescopic rod, the other end of the second telescopic rod is rotatably connected to a hinge shaft, the hinge shaft is fixedly connected to a hinge seat, the hinge seat is fixedly connected to the side wall of the sliding block and is located below the two horizontal connecting rods.

[0016] Preferably, the sealed filter portion includes a filter cartridge, which is coaxially installed in the test tube, a plurality of evenly arranged filter holes are opened on the bottom wall of the filter cartridge, a lap edge is fixedly connected to the top end of the outer wall of the filter cartridge, a connecting cylinder is detachably installed on the top end of the inner part of the filter cartridge, and two relatively arranged rubber blocks are fixedly connected to the bottom end of the connecting cylinder.

[0017] Preferably, the rotation driving part includes a first motor, the first motor is fixedly connected to the inner bottom wall of the outer shell, the output shaft of the first motor is fixedly connected to a driving bevel gear, the driving bevel gear is meshed with a driven bevel gear, the driven bevel gear and the driving bevel gear are perpendicular to each other, and a driving shaft is coaxially fixedly connected inside the driven bevel gear, the driving shaft is vertically arranged and the bottom end is rotatably connected to the inner bottom wall of the outer shell, and the top end of the driving shaft is coaxially fixedly connected to the center of the bottom end of the bottom rotating disk.

[0018] Preferably, the variable temperature preservation mechanism includes a top rotating disk, the middle part of the bottom end of the top rotating disk is coaxially fixedly connected to the top end of the first telescopic rod, the top rotating disk is coaxially rotatably arranged in the outer shell, a plurality of first funnels are fixedly connected to the outer edge of the top rotating disk, the plurality of first funnels are equally spaced along the circumference of the top rotating disk, the first funnels correspond one-to-one to the test tubes, a heating ring is fixedly sleeved on the outer side wall of the first funnel, the bottom end of the first funnel is fixedly connected to a discharge pipe, and an electrically controlled valve is arranged on the discharge pipe.

[0019] Preferably, the pressure crushing mechanism includes a crushing barrel, which is fixedly connected to the top of the outer shell, and a crushing part is fixedly connected to the inner wall of the crushing barrel, and the bottom end of the crushing part is connected to the interior of the outer shell, and the middle part of the top of the crushing barrel is connected to one end of the connecting hose through a first bent tube, and the top of the crushing barrel is also fixedly connected to a vacuum pump and an inflator.

[0020] Preferably, the crushing part includes a second funnel, which is fixedly connected to the inner wall of the crushing barrel, the bottom end of the second funnel is communicated with the interior of the outer shell, the top of the second funnel is fixedly connected to a circular plate, a plurality of evenly arranged through holes are provided on the circular plate, a third motor is fixedly connected to the middle of the bottom end of the circular plate, a collar is fixedly connected to the output shaft of the third motor, a plurality of crushing rods are fixedly connected to the outer wall of the collar, the plurality of crushing rods are equidistantly arranged along the circumference of the collar, the bottom end of the crushing rod is in sliding contact with the top end of the circular plate, the outer cover of the third motor is provided with a protective cylinder, the top end of the protective cylinder is fixedly connected to the bottom end of the circular plate.

[0021] Preferably, the sampling mechanism includes a needle seat, a discharge hole is provided in the middle of the bottom end of the needle seat, the discharge hole is fixedly connected to one end of the second bent tube, the other end of the second bent tube is fixedly connected to the connecting hose, a fourth motor is fixedly embedded in one end of the needle seat, the output shaft of the fourth motor is fixedly connected to one end of a rotating rod, the rotating rod is rotatably arranged in a accommodating groove provided in the needle seat, the end of the accommodating groove away from the fourth motor is coaxially fixedly connected to a sampling needle, the other end of the rotating rod is coaxially rotatably arranged in the sampling needle, the outer side wall of the rotating rod at one end located inside the sampling needle is fixedly connected to a plurality of cutting leaves arranged at equal intervals, a retaining ring is rotatably connected to the middle of the outer side wall of the rotating rod, the retaining ring is fixedly connected to the inner side wall of the accommodating groove, and the retaining ring is flush with the side wall of the discharge hole close to the fourth motor.

[0022] Compared with the prior art, the present invention has the following advantages and technical effects:

[0023] The present invention can perform sampling operations on tumor cell clusters through the sampling mechanism provided. The obtained samples enter the pressure crushing mechanism through the connecting hose, and the samples are crushed by the pressure crushing mechanism. The crushed samples enter the variable temperature preservation mechanism for temporary preservation. The variable temperature preservation mechanism can be adjusted to different preservation temperatures to improve the temporary preservation effect of the samples. The provided filtering sampling mechanism can perform rotational oscillation filtering on the temporarily preserved samples, so that tumor cells can be sorted more quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative labor:

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 It is a schematic diagram of the internal structure of the present invention;

[0027] Figure 3 for Figure 2 A partial enlarged view of middle A;

[0028] Figure 4 It is a schematic diagram of the filter cartridge of the present invention;

[0029] Figure 5 It is a schematic diagram of the connecting tube of the present invention;

[0030] Figure 6 It is a schematic diagram of the internal structure of the present invention from another angle;

[0031] Figure 7 The structure of the crushing part of the present invention is shown in FIG. Figure 1 ;

[0032] Figure 8 The structure of the crushing part of the present invention is shown in FIG. Figure 2 ;

[0033] Fig. 9 for Figure 8 A partial enlarged view of B in the middle;

[0034] Fig.10 It is a cross-sectional view of the sampling mechanism of the present invention.

[0035] Among them, 1. shell; 2. crushing barrel; 3. adding liquid barrel; 4. first bending tube; 5. connecting hose; 6. second bending tube; 7. needle seat; 8. sampling needle; 9. vacuum pump; 10. inflator; 11. sampling hole; 12. bottom rotating disk; 13. top rotating disk; 14. first motor; 15. active bevel gear; 16. driven bevel gear; 17. driving shaft; 18. first telescopic rod; 19. second motor; 20. lead screw; 21. fixed seat; 22. sliding block; 23. fixed cylinder; 24. rotating lug; 25. horizontal connecting rod; 26. vertical connecting rod rod; 27, rotating shaft; 28, second telescopic rod; 29, hinge shaft; 30, hinge seat; 31, test tube; 32, overlapping edge; 33, connecting cylinder; 34, first funnel; 35, heating ring; 36, discharge pipe; 37, electric control valve; 38, second funnel; 39, third motor; 40, sleeve ring; 41, breaking rod; 42, protective cylinder; 43, circular plate; 44, through hole; 45, receiving groove; 46, fourth motor; 47, rotating rod; 48, retaining ring; 49, discharge hole; 50, cutting leaf; 51, filter cylinder; 52, filter hole; 53, rubber block. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0038] Reference Figures 1 to 10 As shown, the present invention provides an integrated machine for sorting, filtering and sampling tumor cell clusters, comprising:

[0039] The housing 1 has a plurality of sampling holes 11 arranged at equal intervals in the middle of the side wall of the housing 1;

[0040] A filtering and sampling mechanism, which is arranged at the lower part of the housing 1;

[0041] The variable temperature storage mechanism is arranged at the upper part of the housing 1 and corresponds to the filtering and sampling mechanism up and down;

[0042] A pressure crushing mechanism, which is fixedly connected to the top of the outer shell 1 and communicated with the inside of the shell 1;

[0043] The sampling mechanism is fixedly connected with the pressure crushing mechanism through a connecting hose 5.

[0044] Furthermore, an additive liquid barrel 3 is fixedly connected to the top of the outer shell 1 , and the additive liquid barrel 3 is communicated with the interior of the outer shell 1 .

[0045] The provided sampling mechanism can be used to sample tumor cell clusters. The obtained samples enter the pressure crushing mechanism through the connecting hose 5, and the samples are crushed by the pressure crushing mechanism. The crushed samples enter the variable temperature preservation mechanism for temporary preservation. The variable temperature preservation mechanism can be adjusted to different preservation temperatures to improve the temporary preservation effect of the samples. The provided filtering sampling mechanism can perform rotational oscillation filtering on the temporarily preserved samples, so that tumor cells can be sorted more quickly.

[0046] A further optimized solution is provided, wherein the filtering sampling mechanism comprises a rotating driving portion, which is fixedly connected to the inner bottom wall of the outer shell 1; the output end of the rotating driving portion is coaxially fixedly connected to a bottom rotating disk 12; the bottom rotating disk 12 is coaxially rotatably arranged inside the outer shell 1; a plurality of moving portions are fixedly connected to the top end of the bottom rotating disk 12; the plurality of moving portions are equally spaced along the circumference of the bottom rotating disk 12; a test tube clamping portion is fixedly connected to the top end of the moving portion; the bottom end of a vertically arranged first telescopic rod 18 is fixedly connected to the center of the top end of the bottom rotating disk 12; and a variable temperature preservation mechanism is fixedly connected to the top end of the first telescopic rod 18.

[0047] A further optimized solution is that the moving part includes a second motor 19, which is fixedly connected to the top of the bottom rotating disk 12, and the output shaft of the second motor 19 is fixedly connected to one end of a horizontally arranged lead screw 20, and the other end of the lead screw 20 is rotatably connected to a fixed seat 21, and the bottom end of the fixed seat 21 is fixedly connected to the top of the bottom rotating disk 12, and the lead screw 20 is threadedly connected to a sliding block 22, and the sliding block 22 is vertically arranged and the bottom end is in sliding contact with the top of the bottom rotating disk 12, and the test tube clamping part is fixedly connected to the top of the side wall of the sliding block 22 away from the first telescopic rod 18.

[0048] The second motor 19 drives the lead screw 20 to rotate, so as to adjust the position of the sliding block 22. During the rotation of the cells, the sliding block 22 is located inside the outer shell 1. After the rotation filtration is completed, the sliding block 22 moves to a position close to the sampling hole 11, and the test tube clamping part is transported to the outside of the outer shell 1. The staff can take the cells away for the next step of processing.

[0049] To further optimize the solution, the test tube clamping part includes two horizontal connecting rods 25, the two horizontal connecting rods 25 are horizontally spaced and one end is fixedly connected to the side wall of the sliding block 22, the other ends of the two horizontal connecting rods 25 are rotatably connected to the top of the fixed cylinder 23 through a rotating ear 24, the test tube 31 is detachably connected in the fixed cylinder 23, and the sealing filter part is detachably installed at the opening position of the test tube 31, the bottom end of the fixed cylinder 23 is fixedly connected to the top of two spaced vertical connecting rods 26, a rotating shaft 27 is fixedly connected between the bottom ends of the two vertical connecting rods 26, the rotating shaft 27 is rotatably connected to one end of the second telescopic rod 28, the other end of the second telescopic rod 28 is rotatably connected to a hinge shaft 29, the hinge shaft 29 is fixedly connected to a hinge seat 30, the hinge seat 30 is fixedly connected to the side wall of the sliding block 22 and is located below the two horizontal connecting rods 25.

[0050] A further optimized solution is that the sealed filter portion includes a filter cartridge 51, which is coaxially installed in the test tube 31. The bottom wall of the filter cartridge 51 is provided with a plurality of evenly arranged filter holes 52. The top end of the outer wall of the filter cartridge 51 is fixedly connected with an overlapping edge 32. The top end of the inner part of the filter cartridge 51 is detachably installed with a connecting cartridge 33, and the bottom end of the connecting cartridge 33 is fixedly connected with two relatively arranged rubber blocks 53.

[0051] The rubber block 53 itself is elastic. When the cell fluid is injected into the test tube 31, the bottom end of the temperature-variable storage mechanism pushes the two rubber blocks 53 open, and then the cell fluid is smoothly injected into the filter cartridge 51. After the injection is completed, the temperature-variable storage mechanism leaves the two rubber blocks 53. The two rubber blocks 53 re-seal the filter cartridge 51 under the action of their own elasticity. Then the rotary drive unit drives the test tube 31 and the filter cartridge 51 to rotate at a high speed. During the rotation, the filter holes 52 filter the cell fluid, and the filtered cell fluid enters the test tube 31. During the rotation filtration process, the second telescopic rod 28 continuously retracts and retracts, thereby adjusting the inclination angle of the test tube 31 and playing an oscillating role. The oscillation and rotation are performed simultaneously to improve the filtering effect.

[0052] A further optimized solution is that the rotation drive part includes a first motor 14, the first motor 14 is fixedly connected to the inner bottom wall of the outer shell 1, the output shaft of the first motor 14 is fixedly connected to a driving bevel gear 15, the driving bevel gear 15 is meshed with a driven bevel gear 16, the driven bevel gear 16 and the driving bevel gear 15 are perpendicular to each other, and a driving shaft 17 is coaxially fixedly connected inside the driven bevel gear 16, the driving shaft 17 is vertically arranged and the bottom end is rotatably connected to the inner bottom wall of the outer shell 1, and the top end of the driving shaft 17 is coaxially fixedly connected to the bottom center of the bottom rotating disk 12.

[0053] A further optimized solution is provided, in which the variable temperature preservation mechanism comprises a top rotating disk 13, the middle part of the bottom end of the top rotating disk 13 is coaxially fixedly connected to the top end of the first telescopic rod 18, the top rotating disk 13 is coaxially rotatably arranged in the outer shell 1, a plurality of first funnels 34 are fixedly connected to the outer edge of the top rotating disk 13, the plurality of first funnels 34 are arranged at equal intervals along the circumference of the top rotating disk 13, the first funnels 34 correspond to the test tubes 31 one by one, a heating ring 35 is fixedly sleeved on the outer side wall of the first funnel 34, the bottom end of the first funnel 34 is fixedly connected to a discharge pipe 36, and an electric-controlled valve 37 is arranged on the discharge pipe 36.

[0054] The cells crushed by the pressure crushing mechanism are respectively injected into each first funnel 34. The heating ring 35 controls the temperature of each first funnel 34 at different temperatures, so that the cell fluid can be temporarily stored at different temperatures. During the temporary storage, the additive liquid in the additive liquid barrel 3 is also injected into the cell fluid. After the temporary storage is completed, the first telescopic rod 18 is shortened to drive the top rotating disk 13 to descend, so that the discharge pipe 36 enters the connecting cylinder 33 and pushes open the two rubber blocks 53. The electric control valve 37 is opened to inject the cell mixture into the filter cylinder 51.

[0055] A further optimized solution is that the pressure crushing mechanism includes a crushing barrel 2, which is fixedly connected to the top of the outer shell 1, and a crushing part is fixedly connected to the inner wall of the crushing barrel 2. The bottom end of the crushing part is connected to the inside of the outer shell 1, and the middle part of the top of the crushing barrel 2 is connected to one end of the connecting hose 5 through the first bent tube 4. The top of the crushing barrel 2 is also fixedly connected to a vacuum pump 9 and an inflator 10.

[0056] A further optimized solution is provided, in which the crushing part comprises a second funnel 38, the second funnel 38 is fixedly connected to the inner wall of the crushing barrel 2, the bottom end of the second funnel 38 is communicated with the inside of the outer shell 1, a circular plate 43 is fixedly connected to the top of the second funnel 38, a plurality of evenly arranged through holes 44 are provided on the circular plate 43, a third motor 39 is fixedly connected to the middle part of the bottom end of the circular plate 43, a collar 40 is fixedly connected to the output shaft of the third motor 39, a plurality of crushing rods 41 are fixedly connected to the outer wall of the collar 40, the plurality of crushing rods 41 are arranged at equal intervals along the circumference of the collar 40, the bottom end of the crushing rod 41 is in sliding contact with the top end of the circular plate 43, a protective cylinder 42 is provided on the outer cover of the third motor 39, and the top end of the protective cylinder 42 is fixedly connected to the bottom end of the circular plate 43.

[0057] After the sample obtained by the sampling mechanism falls on the circular plate 43, the inflator 10 works to inflate the crushing barrel 2, increase the pressure in the crushing barrel 2, and squeeze the sample onto the circular plate 43. At the same time, the third motor 39 drives the crushing rod 41 to rotate at high speed to push the sample, and the sample is crushed in combination with the gas pressure and the through hole 44. The side wall of the crushing rod 41 is set as an inclined surface, and the inclined surface faces the circular plate 43, which can improve the crushing effect of the sample.

[0058] A further optimized solution is provided, wherein the sampling mechanism comprises a needle seat 7, a discharge hole 49 is provided in the middle of the bottom end of the needle seat 7, the discharge hole 49 is fixedly connected to one end of the second bent tube 6, the other end of the second bent tube 6 is fixedly connected to the connecting hose 5, a fourth motor 46 is fixedly embedded in one end of the needle seat 7, the output shaft of the fourth motor 46 is fixedly connected to one end of a rotating rod 47, the rotating rod 47 is rotatably arranged in a receiving groove 45 provided in the needle seat 7, one end of the receiving groove 45 away from the fourth motor 46 is coaxially fixedly connected to a sampling needle 8, the other end of the rotating rod 47 is coaxially rotatably arranged in the sampling needle 8, a plurality of cutting leaves 50 arranged at equal intervals are fixedly connected to the outer side wall of the rotating rod 47 at one end of the rotating rod 47 located inside the sampling needle 8, a retaining ring 48 is rotatably connected to the middle of the outer side wall of the rotating rod 47, the retaining ring 48 is fixedly connected to the inner side wall of the receiving groove 45, and the retaining ring 48 is flush with the side wall of the discharge hole 49 close to the fourth motor 46.

[0059] When sampling, the tip of the sampling needle 8 is inserted into the tumor cell cluster, and then the fourth motor 46 and the vacuum pump 9 are started. The vacuum pump 9 provides negative pressure for the inside of the sampling needle 8. At the same time, the fourth motor 46 drives the cutting blade 50 to rotate at high speed to initially crush the cell cluster. The crushed cell cluster enters the sampling needle 8 under the action of negative pressure, and finally enters the crushing barrel 2 through the accommodating groove 45, the discharge hole 49, the second bent tube 6, the connecting hose 5, and the first bent tube 4 for the next step of processing.

[0060] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0061] The embodiments described above are only descriptions of the preferred embodiments of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope of the present invention.

Claims

1. A tumor cell cluster sorting, filtering and sampling integrated machine, characterized in that: include A housing (1), wherein a plurality of sampling holes (11) arranged at equal intervals are provided in the middle of a side wall of the housing (1); A filtering and sampling mechanism, the filtering and sampling mechanism being arranged at the lower part of the interior of the housing (1); A variable temperature storage mechanism, the variable temperature storage mechanism being arranged at the upper part of the interior of the housing (1) and corresponding to the filtering and sampling mechanism in the upper and lower parts; A pressure crushing mechanism, the pressure crushing mechanism is fixedly connected to the top end of the outer shell (1) and communicated with the interior of the outer shell (1); A sampling mechanism, wherein the sampling mechanism is fixedly connected to the pressure crushing mechanism via a connecting hose (5).

2. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 1, characterized in that: The filtering sampling mechanism comprises a rotating driving part, the rotating driving part is fixedly connected to the inner bottom wall of the housing (1), the output end of the rotating driving part is coaxially fixedly connected to a bottom rotating disk (12), the bottom rotating disk (12) is coaxially rotatably arranged inside the housing (1), the top end of the bottom rotating disk (12) is fixedly connected to a plurality of moving parts, the plurality of moving parts are arranged at equal intervals along the circumference of the bottom rotating disk (12), the top end of the moving part is fixedly connected to a test tube clamping part, the top center of the bottom rotating disk (12) is fixedly connected to the bottom end of a vertically arranged first telescopic rod (18), and the variable temperature storage mechanism is fixedly connected to the top end of the first telescopic rod (18).

3. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 2, characterized in that: The moving part comprises a second motor (19), the second motor (19) is fixedly connected to the top of the bottom rotating disk (12), the output shaft of the second motor (19) is fixedly connected to one end of a horizontally arranged lead screw (20), the other end of the lead screw (20) is rotatably connected to a fixed seat (21), the bottom end of the fixed seat (21) is fixedly connected to the top of the bottom rotating disk (12), the lead screw (20) is threadedly connected to a sliding block (22), the sliding block (22) is vertically arranged and the bottom end thereof is in sliding contact with the top of the bottom rotating disk (12), and the test tube clamping part is fixedly connected to the top of the side wall of the sliding block (22) away from the first telescopic rod (18).

4. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 3, characterized in that: The test tube clamping part comprises two horizontal connecting rods (25), the two horizontal connecting rods (25) are arranged horizontally at intervals and one end of the two horizontal connecting rods (25) is fixedly connected to the side wall of the sliding block (22), the other ends of the two horizontal connecting rods (25) are rotatably connected to the top of the fixed cylinder (23) through a rotating lug (24), the fixed cylinder (23) is detachably connected with a test tube (31), the opening position of the test tube (31) is detachably installed with a sealing filter part, and the bottom end of the fixed cylinder (23) is fixedly connected with A rotating shaft (27) is fixedly connected between the top ends of the two vertical connecting rods (26) arranged at intervals, and the bottom ends of the two vertical connecting rods (26) are fixedly connected. The rotating shaft (27) is rotatably connected to one end of a second telescopic rod (28), and the other end of the second telescopic rod (28) is rotatably connected to a hinge shaft (29). The hinge shaft (29) is fixedly connected to a hinge seat (30). The hinge seat (30) is fixedly connected to the side wall of the sliding block (22) and is located below the two horizontal connecting rods (25).

5. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 4, characterized in that: The sealed filter portion comprises a filter cartridge (51) which is coaxially mounted in the test tube (31); a plurality of evenly arranged filter holes (52) are provided on the bottom wall of the filter cartridge (51); a lap edge (32) is fixedly connected to the top end of the outer wall of the filter cartridge (51); a connecting cartridge (33) is detachably mounted on the top end of the inner part of the filter cartridge (51); and two rubber blocks (53) arranged opposite to each other are fixedly connected to the bottom end of the connecting cartridge (33).

6. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 2, characterized in that: The rotary drive unit comprises a first motor (14), the first motor (14) being fixedly connected to the inner bottom wall of the housing (1), the output shaft of the first motor (14) being fixedly connected to a driving bevel gear (15), the driving bevel gear (15) being meshed with a driven bevel gear (16), the driven bevel gear (16) being perpendicular to the driving bevel gear (15), the interior of the driven bevel gear (16) being coaxially fixedly connected to a driving shaft (17), the driving shaft (17) being vertically arranged and the bottom end of which is rotatably connected to the inner bottom wall of the housing (1), the top end of which is coaxially fixedly connected to the center of the bottom end of the bottom rotating disk (12).

7. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 4, characterized in that: The variable temperature preservation mechanism comprises a top rotating disk (13), the middle part of the bottom end of the top rotating disk (13) is coaxially fixedly connected to the top end of the first telescopic rod (18), the top rotating disk (13) is coaxially rotatably arranged in the housing (1), the outer edge of the top rotating disk (13) is fixedly connected to a plurality of first funnels (34), the plurality of first funnels (34) are arranged at equal intervals along the circumference of the top rotating disk (13), the first funnels (34) correspond to the test tubes (31) one by one, the outer wall of the first funnel (34) is fixedly sleeved with a heating ring (35), the bottom end of the first funnel (34) is fixedly connected to a discharge pipe (36), and the discharge pipe (36) is provided with an electric control valve (37).

8. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 1, characterized in that: The pressure crushing mechanism comprises a crushing barrel (2), the crushing barrel (2) is fixedly connected to the top of the outer shell (1), the inner wall of the crushing barrel (2) is fixedly connected with a crushing part, the bottom end of the crushing part is connected to the inside of the outer shell (1), the middle part of the top of the crushing barrel (2) is connected to one end of the connecting hose (5) through a first bent tube (4), and the top of the crushing barrel (2) is also fixedly connected with an air pump (9) and an inflator (10).

9. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 8, characterized in that: The crushing part comprises a second funnel (38), the second funnel (38) is fixedly connected to the inner wall of the crushing barrel (2), the bottom end of the second funnel (38) is communicated with the inside of the shell (1), the top end of the second funnel (38) is fixedly connected to a circular plate (43), the circular plate (43) is provided with a plurality of evenly arranged through holes (44), the middle part of the bottom end of the circular plate (43) is fixedly connected to a third motor (39), the output shaft of the third motor (39) is fixedly connected to a sleeve (40), the outer wall of the sleeve (40) is fixedly connected to a plurality of crushing rods (41), the plurality of crushing rods (41) are arranged at equal intervals along the circumference of the sleeve (40), the bottom ends of the crushing rods (41) are in sliding contact with the top end of the circular plate (43), the outer cover of the third motor (39) is provided with a protective tube (42), the top end of the protective tube (42) is fixedly connected to the bottom end of the circular plate (43).

10. The integrated machine for sorting, filtering and sampling tumor cell clusters according to claim 1, characterized in that: The sampling mechanism comprises a needle seat (7), a discharge hole (49) is provided in the middle of the bottom end of the needle seat (7), the discharge hole (49) is fixedly connected to one end of a second bent tube (6), the other end of the second bent tube (6) is fixedly connected to the connecting hose (5), a fourth motor (46) is fixedly embedded in one end of the needle seat (7), the output shaft of the fourth motor (46) is fixedly connected to one end of a rotating rod (47), the rotating rod (47) is rotatably arranged in a receiving groove (45) provided in the needle seat (7), the receiving groove (45) is away from the One end of the fourth motor (46) is coaxially fixedly connected to a sampling needle (8), and the other end of the rotating rod (47) is coaxially rotatably arranged in the sampling needle (8). The outer wall of one end of the rotating rod (47) located inside the sampling needle (8) is fixedly connected to a plurality of cutting leaves (50) arranged at equal intervals, and a retaining ring (48) is rotatably connected to the middle part of the outer wall of the rotating rod (47). The retaining ring (48) is fixedly connected to the inner wall of the accommodating groove (45), and the retaining ring (48) is flush with the side wall of the discharge hole (49) close to the fourth motor (46).

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