Lysis device for automated nucleic acid extraction

Abstract

The present invention provides a lysis device for automated nucleic acid extraction, comprising: a lysis turntable having an inner and outer disk, the outer disk being disposed around the outer periphery of the inner disk, the outer and inner disks being provided with a plurality of sample initial wells and a plurality of sample processing wells, each corresponding to the other, and being rotatable in opposite first and second directions; a disk cover covering the plurality of sample initial wells and the plurality of sample processing wells, the disk cover being provided with a second sample discharging station, a capping and sample removal station, a sample loading station, a lid opening station, and a first sample loading station, in sequence along a first direction; a second sample loading station, a liquid injection station, a heating station, and a first sample discharging station, in sequence along a second direction; and a liquid injection mechanism, wherein the liquid injection tube is located above the lysis turntable and vertically opposite the liquid injection station. The present invention solves the technical problems of complex structures, cumbersome lysis processes, and slow speeds in nucleic acid extraction lysis devices.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid extraction, and in particular to a lysis device for automated nucleic acid extraction. Background Art

[0002] Nucleic acids are divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is primarily concentrated in the cell nucleus (mitochondria and chloroplasts), while RNA is primarily distributed in the cytoplasm. Nucleic acids, as the material basis for gene expression, are the primary subject of molecular biology research. Whether studying the structure or function of nucleic acids, the nucleic acids in the sample to be tested must first be released from the cell and then separated from other cellular impurities to obtain purer nucleic acids. This process is called nucleic acid extraction and purification. Nucleic acid extraction provides the foundation for a wide range of research and applications.

[0003] There are three main types of nucleic acid extraction methods: solution extraction, column extraction, and magnetic bead purification. The most commonly used method is magnetic bead purification. Figure 1 As shown, a lysis solution is added to a sample tube containing a sample to release nucleic acids from the cell nucleus. Magnetic beads are then added to the sample tube to allow the nucleic acids to adsorb onto the beads. The beads, which are then concentrated on one side of the sample tube by a magnet, are then removed from the sample tube. After the waste liquid is removed from the sample tube, a wash solution is added to wash the nucleic acids. Finally, the waste liquid from the nucleic acid wash is removed and an elution solution is added to separate the nucleic acids from the magnetic beads. Finally, the magnetic beads are removed to obtain purified nucleic acids. The entire process can be simplified as: lysis, washing, and elution.

[0004] Currently, many types of nucleic acid extraction instruments have been developed based on the principle of magnetic bead purification, which are used to extract and purify nucleic acids from test samples. These instruments include those that extract multiple samples simultaneously, those that extract a single sample independently, and those that extract multiple samples on demand. Nucleic acid extraction instruments that can extract nucleic acids from multiple samples simultaneously can complete the extraction of nucleic acids from multiple samples at one time, but they need to collect a sufficient number of samples before they can start running, and cannot be used for on-demand testing; nucleic acid extraction instruments that can extract single samples do not need to collect a sufficient number of samples, but when faced with a situation where the sample volume is too large, their processing capacity is limited and they cannot complete nucleic acid extraction quickly; and nucleic acid extraction instruments that can be used on-demand and collected from multiple samples can, on the one hand, realize on-demand collection of samples without waiting for the collection of a sufficient number of samples, and on the other hand, can also be used in situations where the sample volume is too large, and have good development prospects. However, the existing technology of on-demand and collected nucleic acid extraction instruments is not yet mature, and the development of nucleic acid extraction instruments is still in its early stages. There are many problems. For example, when the nucleic acid of the sample to be tested is lysed, the lysis device has a complex structure and a large size, which makes the lysis process cumbersome and slow, and also causes problems such as high cost. No effective solution has been given yet.

[0005] Therefore, the present invention proposes a lysis device for automated nucleic acid extraction to overcome the defects of the prior art. Summary of the Invention

[0006] The object of the present invention is to provide a lysis device for automated nucleic acid extraction, which can not only realize the on-demand collection of test samples and accelerate the lysis rate of nucleic acid extraction, but also prevent the test samples from being unable to be processed in time when applied to scenarios with huge sample volumes; in addition, the design of the device is more streamlined, the volume is reduced, the cost is lower, and the streamlined structure further simplifies the operation process, improves the lysis rate, and has better use effect.

[0007] The purpose of the present invention can be achieved by adopting the following scheme:

[0008] The present invention provides a lysis device for automated nucleic acid extraction, the lysis device for automated nucleic acid extraction comprising:

[0009] A lysis turntable comprising an inner disk body and an outer disk body, the outer disk body being annularly arranged around the outer circumference of the inner disk body, the outer disk body being provided with a plurality of sample initial holes for accommodating sample tubes at intervals along its circumference, and the inner disk body being provided with a plurality of sample processing holes for accommodating the sample tubes at intervals along its circumference, wherein the plurality of sample initial holes correspond one-to-one with the plurality of sample processing holes in a radial direction of the lysis turntable, and the outer disk body and the inner disk body being rotatable in a first direction and a second direction, respectively, the first direction being opposite to the second direction;

[0010] The lysis turntable further comprises a disk cover, the disk cover being located above the inner disk body and the outer disk body, and the disk cover covering the plurality of sample initial holes and the plurality of sample processing holes, the disk cover being sequentially provided with a second sample discharging station, a capping and sample separation station, a sample loading station, a cover opening station and a first sample loading station along the first direction, and the distances between the second sample discharging station, the capping and sample separation station, the sample loading station, the cover opening station and the first sample loading station and the central axis of the outer disk body are all equal to the distance between the sample initial holes and the central axis of the outer disk body; the disk cover being sequentially provided with a second sample loading station, a liquid injection station, a heating station and a first sample discharging station along the second direction, and the distances between the second sample loading station, the liquid injection station, the heating station and the first sample discharging station and the central axis of the inner disk body are all equal to the distance between the sample processing holes and the central axis of the inner disk body;

[0011] The liquid injection mechanism includes a liquid injection tube for outputting magnetic bead lysis solution, and the liquid injection tube is located above the lysis turntable and vertically opposite to the liquid injection station.

[0012] In a preferred embodiment of the present invention, when the outer disk rotates in the first direction, the sample tube containing the sample to be lysed is placed from the sample loading station into any of the sample initial wells vertically opposite thereto, and the sample tube is uncapped when the sample tube rotates with the outer disk to be vertically opposite to the uncapping station.

[0013] When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the first sampling station, the sample tube is transferred from the sample initial hole to any of the sample processing holes vertically opposite to the second sampling station.

[0014] In a preferred embodiment of the present invention, when the sample tube rotates along the second direction with the inner disk to a position vertically opposite to the injection station, the magnetic bead lysis solution is injected into the sample tube through the injection tube, and the sample tube is heated at least when the sample tube rotates along the inner disk to a position vertically opposite to the heating station.

[0015] When the sample tube rotates along the second direction with the inner disk to be vertically opposite to the first sample output station, the sample tube is transferred from the first sample output station to any of the sample initial holes vertically opposite to the second sample output station.

[0016] In a preferred embodiment of the present invention, when the sample tube rotates along the first direction with the outer disk to be vertically opposite to the capping and sample separation station, the capping and sample removal process is performed on the sample tube.

[0017] In a preferred embodiment of the present invention, the second sample discharging station, the capping and sample separation station, the sample loading station, the cover opening station, the first sample injection station, the second sample injection station, the liquid injection station, the heating station and the first sample discharging station are all through holes opened on the disk cover and are all located within the same semicircle of the disk cover.

[0018] In a preferred embodiment of the present invention, the first sample introduction station and the second sample introduction station are located on an extension line of the same radius of the disk cover;

[0019] And / or, the sample loading station and the heating station are located on an extension line of the same radius of the plate cover;

[0020] And / or, the second sample discharging station and the first sample discharging station are located on an extension line of the same radius of the disk cover.

[0021] In a preferred embodiment of the present invention, the cracking turntable further comprises a bottom plate with an opening at the top and a sealing bottom, wherein the bottom plate comprises a receiving space communicating with the top opening of the bottom plate, wherein the inner plate body and the outer plate body are rotatably disposed in the receiving space, respectively, and a fixed shaft is fixed in the receiving space, wherein the inner plate body is arranged around the outer periphery of the fixed shaft.

[0022] The liquid injection mechanism is arranged on the top of the disc cover;

[0023] Alternatively, the disc cover is annular, and is arranged on the top opening of the base plate and is arranged around the outer circumference of the fixed shaft, and the liquid injection mechanism is arranged on the top of the fixed shaft.

[0024] In a preferred embodiment of the present invention, the disk cover is provided with a plurality of first through holes spaced apart along its circumference, the distance between the first through holes and the central axis of the outer disk body being equal to the distance between the sample initial hole and the central axis of the outer disk body, and the angle between two adjacent first through holes in the circumferential direction of the disk cover being equal to the angle between two adjacent sample initial holes in the circumferential direction of the outer disk body;

[0025] The disk cover is provided with a plurality of second through holes spaced apart along its circumference, wherein the distance between the second through holes and the central axis of the inner disk body is equal to the distance between the sample processing hole and the central axis of the inner disk body, and the angle between two adjacent second through holes in the circumferential direction of the disk cover is equal to the angle between two adjacent sample processing holes in the circumferential direction of the inner disk body;

[0026] The disc cover is provided with a plurality of hole position identification parts, and the plurality of hole position identification parts are used to mark the number and position of the plurality of first through holes and / or the plurality of second through holes.

[0027] In a preferred embodiment of the present invention, the injection mechanism further includes a robotic arm and a liquid storage container, the liquid outlet of the liquid storage container is connected to the injection tube via an infusion line, and the infusion line is provided with a pumping motor, which is used to pump the magnetic bead lysate stored in the liquid storage container to the injection tube;

[0028] The fixed end of the robotic arm is connected to the lysis turntable, and the injection tube is arranged at the movable end of the robotic arm to adjust the position of the injection tube.

[0029] In a preferred embodiment of the present invention, the robotic arm includes a mounting base, the liquid storage container is arranged on the mounting base, and the mounting base is provided with a vertical pole, a vertically movable lifting arm is provided on the vertical pole, a cross arm is provided on the top of the lifting arm, and the pumping motor and the injection pipe are both provided on the cross arm;

[0030] A guide rail extending vertically is provided on the vertical pole, the lifting arm can be slidably connected to the guide rail, a driving motor is provided on the mounting base, the driving shaft of the driving motor is vertically upward and connected to the bottom end of the vertically arranged screw rod, the horizontal arm is connected to the top end of the lifting arm, and a connecting block is provided at the bottom end of the lifting arm, the connecting block has a threaded hole, and the connecting block is screwed to the screw rod through the threaded hole.

[0031] As described above, the characteristics and advantages of the lysis device for automated nucleic acid extraction of the present invention are:

[0032] During operation, the sample tube can be placed into the sample initial hole vertically opposite to it through the sample loading station, and the sample tube is uncapped when it is rotated to the uncapping station (so that magnetic bead lysis solution can be injected into the sample tube in the subsequent operation); thereafter, the sample tube is transferred from the first sample loading station to the second sample loading station, so that the sample tube can be rotated along the second direction with the inner disk body; when the sample tube is rotated along the second direction to the liquid injection station, the magnetic bead lysis solution is injected into the uncapped sample tube through the liquid injection tube, and the sample tube is heated at least in the subsequent heating station to increase the lysis rate of the nucleic acid; thereafter, the sample tube is transferred from the first sample discharging station to the second sample discharging station, so that the sample tube can be rotated along the first direction with the outer disk body again When the sample tube rotates along the first direction to the capping and sample removal station, the cap of the sample tube can be removed to complete the lysis processing of the sample in the sample tube. During the whole process, the sample tube can accurately find the corresponding station and complete the corresponding operation, which is faster and more accurate, and realizes the on-demand collection of samples to be lysed, thereby accelerating the lysis rate of nucleic acid extraction. Due to the setting of multiple porous positions (i.e., multiple sample initial holes and multiple sample processing holes), when applied to scenarios with huge sample volumes, the situation of samples to be lysed not being unable to be processed in time will not occur; in addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of its structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has better use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The following drawings are only intended to illustrate and explain the present invention, and are not intended to limit the scope of the present invention.

[0034] in:

[0035] Figure 1 : It is the operational flow chart of the magnetic bead purification method in the prior art.

[0036] Figure 2 : One of the three-dimensional diagrams of the lysis device for automated nucleic acid extraction of the present invention.

[0037] Figure 3 : A top view of the internal structure of the lysis turntable in the lysis device for automated nucleic acid extraction of the present invention.

[0038] Figure 4 :for Figure 2 A partial enlarged view of position A in the middle.

[0039] Figure 5 :for Figure 2 A partial enlarged view of position B in the middle.

[0040] Figure 6 :for Figure 2 A partial enlarged view of the C position in the middle.

[0041] Figure 7 :for Figure 2 A partial enlarged view of position D in the middle.

[0042] Figure 8 : It is a structural schematic diagram of the liquid injection mechanism in the lysis device for automated nucleic acid extraction of the present invention.

[0043] Figure 9 : This is the second stereoscopic diagram of the lysis device for automated nucleic acid extraction of the present invention.

[0044] The accompanying drawings in the present invention are:

[0045] 1. Liquid injection mechanism; 101. Mounting base;

[0046] 102. Driving motor; 103. Liquid storage container;

[0047] 104, upright pole; 1041, guide rail;

[0048] 105. Lifting arm; 1051. Connecting block;

[0049] 106. Screw rod; 107. Cross arm;

[0050] 108. Pumping motor; 109. Liquid injection pipe;

[0051] 2. Cracking turntable; 201. Chassis;

[0052] 2011, inner plate; 2012, outer plate;

[0053] 2013, sample initial well; 2014, sample processing well;

[0054] 2015, sample tube holder; 202, tray cover;

[0055] 2021, first sample loading station; 2022, second sample loading station;

[0056] 2023, liquid filling station; 2024, cover opening station;

[0057] 2025, sample loading station; 2026, heating station;

[0058] 2027, capping and sample removal station; 2028, second sample removal station;

[0059] 2029, first sample output station; 2030, first through hole;

[0060] 2031, second through hole; 2032, hole position marking portion;

[0061] 203. Fixed axis; 3. Sample tube. DETAILED DESCRIPTION

[0062] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0063] The present invention aims to provide a lysis device for automated nucleic acid extraction that is easy to operate, has a fast lysis rate, is integrated, streamlined, and reduces costs. The device is used for Figure 1 The nucleic acid is lysed in the sample to be tested to release the nucleic acid.

[0064] The lysis device for automated nucleic acid extraction of the present invention can, on the one hand, realize the on-demand collection of test samples, accelerate the lysis rate of nucleic acid extraction, and at the same time, when the application scenario is a huge amount of samples, the test samples will not be unable to be processed in time; on the other hand, the structural design of the lysis device is simpler, the volume is reduced, and the cost is reduced. At the same time, due to its streamlined structure, the operation process is further simplified, and the lysis rate is effectively improved.

[0065] like Figures 2 to 9 As shown, the present invention provides a lysis device for automated nucleic acid extraction, the lysis device for automated nucleic acid extraction comprises: a lysis turntable 2, the lysis turntable 2 having an inner disk body 2011 and an outer disk body 2012, both of which are annular, the outer disk body 2012 being arranged around the outer circumference of the inner disk body 2011, a plurality of sample initial holes 2013 for accommodating sample tubes 3 (the sample tubes 3 are pre-filled with samples to be lysed) being arranged on the outer disk body 2012 and spaced evenly along the circumference of the outer disk body 2012, and the inner disk body 2011 having a plurality of sample initial holes 2013 for accommodating sample tubes 3 (the sample tubes 3 are pre-filled with samples to be lysed). Multiple sample processing holes 2014 for accommodating sample tubes 3 are evenly spaced along the circumference of the inner disk body 2011. Along the radial direction of the lysis turntable 2, the multiple sample initial holes 2013 correspond to the multiple sample processing holes 2014 one by one, that is, the multiple sample initial holes 2013 and the corresponding sample processing holes 2014 are located on the same radial extension line of the lysis turntable 2. The outer disk body 2012 can rotate in a first direction, while the inner disk body 2011 can rotate in a second direction. The first direction and the second direction are opposite directions; wherein the first direction can be Figure 2 The second direction can be the counterclockwise direction of Figure 2 Clockwise direction in .

[0066] The lysis turntable 2 also includes a disk cover 202, which is located above the inner disk body 2011 and the outer disk body 2012, and covers a plurality of sample initial holes 2013 and a plurality of sample processing holes 2014. The disk cover 202 is provided with a second sample discharging station 2028, a capping and sample separation station 2027, a sample loading station 2025, a cover opening station 2024 and a first sample loading station 2021 in sequence along the first direction, and the second sample discharging station 2028 is provided. The distances between the central axis of the outer plate 2012 and the sample initial hole 2013 are equal to the distance between the sample initial hole 2013 and the central axis of the outer plate 2012. During the rotation of the outer plate 2012, any sample initial hole 2013 can be rotated to the position close to the second sample discharging station 2028, the sample discharging station 2027, the sample loading station 2025, the decapping station 2024 and the first sample loading station 2021. , the opening station 2024 and the first sample introduction station 2021 are vertically opposite to each other; the second sample introduction station 2022, the liquid injection station 2023, the heating station 2026 and the first sample discharge station 2029 are sequentially arranged on the disk cover 202 along the second direction, and the distances between the second sample introduction station 2022, the liquid injection station 2023, the heating station 2026 and the first sample discharge station 2029 and the central axis of the inner disk body 2011 are all equal to the distance between the sample processing hole 2014 and the central axis of the inner disk body 2011. During the rotation of the inner disk body 2011, any sample processing hole 2014 can be rotated to a position vertically opposite to the second sample introduction station 2022, the liquid injection station 2023, the heating station 2026 and the first sample discharge station 2029, so that different operations are performed on the vertically opposite sample tubes 3 at different stations, and finally the purpose of completing the lysis of the sample to be lysed in the sample tube 3 is achieved;

[0067] like Figure 2 、 Figure 8 、 Figure 9 As shown, the lysis device for automated nucleic acid extraction further includes an injection mechanism 1 , which includes an injection tube 109 for outputting magnetic bead lysis solution. The injection tube 109 is located above the lysis turntable 2 and vertically opposite to the injection station 2023 .

[0068] In the present invention, the lysis turntable 2 has an inner disk body 2011 and an outer disk body 2012 that can rotate in opposite directions (that is, the outer disk body 2012 and the inner disk body 2011 can rotate in opposite first and second directions respectively), and the outer disk body 2012 and the inner disk body 2011 are respectively spaced and evenly arranged along their respective circumferential directions with multiple sample initial holes 2013 and multiple sample processing holes 2014 that can accommodate sample tubes 3; a disk cover 202 is covered above the multiple sample initial holes 2013 and the multiple sample processing holes 2014, and the disk cover 202 is sequentially arranged along the first direction with a second sample discharge station 2028, a capping and sample separation station 2027, a sample loading station 2025, a cover opening station 2024 and a first sample loading station 2021, and the second sample discharge station The sample station 2028, the capping and sample separation station 2027, the loading station 2025, the decapping station 2024 and the first loading station 2021 are all at equal radial distances from the sample initial hole 2013. The second loading station 2022, the injection station 2023, the heating station 2026 and the first sample outlet station 2029 are sequentially arranged on the disk cover 202 along the second direction. The second loading station 2022, the injection station 2023, the heating station 2026 and the first sample outlet station 2029 are all at equal radial distances from the sample processing hole 2014. During operation, the sample tube 3 can be placed into the sample initial hole 2013 vertically opposite to it through the loading station 2025, and the sample tube 3 can be placed in the sample initial hole 2013 vertically opposite to it when it is rotated to the decapping station 2024. The lid is opened (so that magnetic bead lysis solution can be injected into the sample tube 3 in the subsequent operation); thereafter, the sample tube 3 is transferred from the first sample injection station 2021 to the second sample injection station 2022, so that the sample tube 3 can rotate along the second direction with the inner disk 2011. When the sample tube 3 rotates along the second direction to the liquid injection station 2023, the magnetic bead lysis solution is injected into the opened sample tube 3 through the liquid injection tube 109 of the liquid injection mechanism 1, and the sample tube 3 is heated at least in the subsequent heating station 2026 to increase the lysis rate of the nucleic acid; thereafter, the sample tube 3 is transferred from the first sample output station 2029 to the second sample output station 2028, so that the sample tube 3 can rotate along the first direction with the outer disk 2012 again. When the sample tube 3 rotates along the second direction to the liquid injection station 2023, the magnetic bead lysis solution is injected into the opened sample tube 3 through the liquid injection tube 109 of the liquid injection mechanism 1, and the sample tube 3 is heated at least in the subsequent heating station 2026 to increase the lysis rate of the nucleic acid; When the first direction is rotated to the capping and sample removal station 2027, the cap of the sample tube 3 can be removed to complete the lysis processing of the sample in the sample tube 3. During the whole process, the sample tube 3 can accurately find the corresponding station and complete the corresponding operation, which is faster and more accurate, and realizes the on-demand collection of samples to be lysed, thereby accelerating the lysis rate of nucleic acid extraction. Due to the setting of multiple porous positions (i.e., multiple sample initial holes 2013 and multiple sample processing holes 2014), when applied to scenarios with huge sample volumes, there will be no situation where the samples to be lysed cannot be processed in time; in addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of its structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has a better use effect.

[0069] In the present invention, Figure 3 As shown, along the radial direction of the lysis turntable 2, the multiple sample initial wells 2013 correspond one-to-one with the multiple sample processing wells 2014, i.e., the multiple sample initial wells 2013 and the corresponding sample processing wells 2014 are located on the same extended radius of the lysis turntable 2. Each sample processing well 2014 on the inner disk body 2011 corresponds to a sample initial well 2013 on the outer disk body 2012, and the angle of each rotation of the inner disk body 2011 is equal to the angle of each rotation of the outer disk body 2012. Therefore, after each complete rotation of the inner disk body 2011 and the outer disk body 2012, the multiple sample processing wells 2014 and the multiple sample initial wells 2013 are still located on the same extended radius.

[0070] Further, such as Figures 4 to 7 As shown, a sample tube holder 2015 is respectively provided in the sample initial hole 2013 and the sample processing hole 2014. The sample tube holder 2015 is cylindrical with an open top. The sample tube holder 2015 is adapted to the sample tube 3 so that the sample tube 3 can be inserted into the sample tube holder 2015 and ensure the stable placement of the sample tube 3.

[0071] Specifically, when the outer disk body 2012 rotates along the first direction, the sample tube 3 containing the sample to be lysed is placed from the sample loading station 2025 into any sample initial hole 2013 vertically opposite thereto, and the sample tube 3 is opened when the sample tube 3 rotates along with the outer disk body 2012 to be vertically opposite to the opening station 2024; when the sample tube 3 rotates along with the outer disk body 2012 along the first direction to be vertically opposite to the first sample injection station 2021, the sample tube 3 is transferred from the sample initial hole 2013 to any sample processing hole 2014 vertically opposite to the second sample injection station 2022.

[0072] Specifically, when the sample tube 3 rotates along the second direction with the inner disk body 2011 to be vertically opposite to the injection station 2023, the magnetic bead lysis solution is injected into the sample tube 3 through the injection tube 109, and the sample tube 3 is heated at least when the sample tube 3 rotates along the inner disk body 2011 to a position vertically opposite to the heating station 2026; when the sample tube 3 rotates along the second direction with the inner disk body 2011 to be vertically opposite to the first sample output station 2029, the sample tube 3 is transferred from the first sample output station 2029 to any sample initial hole 2013 vertically opposite to the second sample output station 2028.

[0073] Specifically, when the sample tube 3 rotates along the first direction with the outer disk 2012 to be vertically opposite to the capping and sample separation station 2027, the capping and sample removal process is performed on the sample tube 3.

[0074] In an optional embodiment of the present invention, a heating element is provided at heating station 2026 to heat sample tube 3 passing through heating station 2026, thereby accelerating the lysis of the sample to be lysed within sample tube 3, rapidly releasing nucleic acids, and improving lysis efficiency. Of course, a heating element may also be provided at any location between the second sample injection station 2022 and the first sample discharge station 2029, so as to heat sample tube 3 to a temperature within a suitable lysis range after the lysis solution is injected. The heating element may be, but is not limited to, an electric heating element (e.g., a heating resistor).

[0075] In an optional embodiment of the present invention, Figure 2 、 Figure 9 As shown, the second sample-discharging station 2028, the capping and sample-separating station 2027, the sample-loading station 2025, the cover-opening station 2024, the first sample-injection station 2021, the second sample-injection station 2022, the liquid-filling station 2023, the heating station 2026 and the first sample-discharging station 2029 are all through holes opened on the disk cover 202, and the second sample-discharging station 2028, the capping and sample-separating station 2027, the sample-loading station 2025, the cover-opening station 2024, the first sample-injection station 2021, the second sample-injection station 2022, the liquid-filling station 2023, the heating station 2026 and the first sample-discharging station 2029 are all located within the same semicircle of the disk cover 202.

[0076] Further, if Figure 4 、 Figure 6As shown, the first sample loading station 2021 and the second sample loading station 2022 are located on an extension line of the same radius of the tray cover 202, and the first sample loading station 2021 and the second sample loading station 2022 are connected, thereby facilitating the transfer of the sample tube 3 from the first sample loading station 2021 to the second sample loading station 2022. The second sample output station 2028 and the first sample output station 2029 are located on an extension line of the same radius of the tray cover 202, and the second sample output station 2028 and the first sample output station 2029 are connected, thereby facilitating the transfer of the sample tube 3 from the first sample output station 2029 to the second sample output station 2028. The positions of the first sample injection station 2021 and the second sample injection station 2022 on the disk cover 202 are 180 degrees apart from the positions of the first sample discharge station 2029 and the second sample discharge station 2028 on the disk cover 202. When the sample tube 3 in the sample initial hole 2013 vertically opposite to the first sample injection station 2021 is transferred to the sample processing hole 2014 vertically opposite to the second sample injection station 2022, it can be determined that there is no sample tube 3 in the sample initial hole 2013 vertically opposite to the first sample injection station 2021. Subsequently, after the inner disk body 2011 rotates 180 degrees, the sample tube 3 is transferred to the sample processing hole 2014 vertically opposite to the second sample injection station 2022. The sample tube 3 in the vertically opposite sample processing hole 2014 is rotated to a position vertically opposite to the first sample output station 2029, thereby ensuring that the sample initial hole 2013 without the sample tube 3 is exactly rotated 180° with the outer disk body 2012 to reach a position vertically opposite to the second sample output station 2028. Therefore, the sample tube 3 can be transferred from the sample processing hole 2014 vertically opposite to the first sample output station 2029 to the sample initial hole 2013 vertically opposite to the second sample output station 2028, avoiding the situation where the sample tube 3 exists in the sample initial hole 2013 vertically opposite to the second sample output station 2028 and cannot be transferred.

[0077] Further, such as Figure 5 As shown, the sample loading station 2025 and the heating station 2026 are located on an extension line of the same radius of the disk cover 202 , and the sample loading station 2025 is connected to the heating station 2026 .

[0078] In an optional embodiment of the present invention, Figure 2 、 Figure 9As shown, the disk cover 202 is provided with a plurality of first through holes 2030 spaced and evenly distributed along its circumference. The distance between the first through holes 2030 and the central axis of the outer disk body 2012 is equal to the distance between the sample initial hole 2013 and the central axis of the outer disk body 2012, and the angle between two adjacent first through holes 2030 in the circumferential direction of the disk cover 202 is equal to the angle between two adjacent sample initial holes 2013 in the circumferential direction of the outer disk body 2012. Therefore, after each rotation of the outer disk body 2012, the plurality of first through holes 2030 are still located at positions corresponding to the plurality of sample initial holes 2013 in the vertical direction. The staff can know the rotation position of the sample tube 3 through the first through holes 2030, and thus know the lysis progress.

[0079] Further, such as Figure 2 、 Figure 9 As shown, a plurality of second through holes 2031 are spaced and evenly distributed along the circumference of the disk cover 202. The distance between the second through holes 2031 and the central axis of the inner disk body 2011 is equal to the distance between the sample processing hole 2014 and the central axis of the inner disk body 2011, and the angle between two adjacent second through holes 2031 in the circumferential direction of the disk cover 202 is equal to the angle between two adjacent sample processing holes 2014 in the circumferential direction of the inner disk body 2011. Therefore, after each rotation of the inner disk body 2011, the plurality of second through holes 2031 are still located at positions corresponding to the plurality of sample processing holes 2014 in the vertical direction. The arrangement has the same function and effect as the first through holes 2030, and will not be elaborated here.

[0080] Further, such as Figure 9 As shown, a plurality of hole position identification portions 2032 may be provided on the top surface of the disk cover 202, and the plurality of hole position identification portions 2032 correspond one-to-one to the plurality of first through holes 2030, and the plurality of hole position identification portions 2032 also correspond one-to-one to the plurality of second through holes 2031. The number and positions of the plurality of first through holes 2030 and / or the plurality of second through holes 2031 may be marked through the plurality of hole position identification portions 2032. The number of sample tubes 3 that can be accommodated by the lysis device of the present invention may be directly known through the hole position identification portions 2032, and thus the disposable sample processing volume may be known.

[0081] In an optional embodiment of the present invention, Figure 2 、 Figure 3 、 Figure 9As shown, the lysis turntable 2 further includes a cylindrical bottom plate 201 with an opening at the top and a sealed bottom. The bottom plate 201 has a storage space that is in communication with the top opening of the bottom plate 201. An inner plate body 2011 and an outer plate body 2012 are rotatably disposed in the storage space. A fixed shaft 203 is also fixed in the storage space. The inner plate body 2011 is rotatably disposed around the outer periphery of the fixed shaft 203. The disk cover 202 is annular and covers the top opening of the bottom plate 201 and surrounds the outer periphery of the fixed shaft 203. The liquid injection mechanism 1 is disposed on top of the fixed shaft 203, thereby providing a mounting location for the liquid injection mechanism 1. Of course, if the disk cover 202 is a disk and the fixed shaft 203 is covered by the disk cover 202 and disposed in the storage space, the liquid injection mechanism 1 can be disposed on top of the disk cover 202.

[0082] In the present invention, the rotation of the inner disk 2011 and the outer disk 2012 can be directly driven by a motor. Alternatively, the motor can provide driving force, and the motor can be used to drive the inner disk 2011 and the outer disk 2012 through a transmission structure such as a screw drive or a gear drive to respectively drive the inner disk 2011 and the outer disk 2012 to rotate. However, it is necessary to ensure that the rotation angle of the inner disk 2011 and the outer disk 2012 is the same per rotation step.

[0083] In a specific embodiment of the present invention, Figure 2 、 Figure 8 、 Figure 9 As shown, the injection mechanism 1 also includes a liquid storage container 103, and the liquid outlet of the liquid storage container 103 is connected to the injection tube 109 through an infusion line. A pumping motor 108 is provided on the infusion line. The pumping motor 108 is used to pump the magnetic bead lysis solution stored in the liquid storage container 103 to the injection tube 109 so as to inject the magnetic bead lysis solution into the corresponding sample tube 3. By controlling the working state of the pumping motor 108, the amount of magnetic bead lysis solution injected into the sample tube 3 can be controlled to improve the accuracy of the injected magnetic bead lysis solution and ensure that the nucleic acid can be quickly and fully released. Among them, the magnetic bead lysis solution is a mixed solution containing magnetic beads and a lysis reagent required for sample lysis, and the specific type of lysis reagent is a prior art. Those skilled in the art can select it according to the sample that actually needs to be lysed, and it is not limited here.

[0084] In a specific embodiment of the present invention, Figure 2 、 Figure 8 、 Figure 9As shown, the injection mechanism 1 also includes a robotic arm having a fixed end and a movable end. The fixed end of the robotic arm is connected to the lysis turntable 2, and the injection tube 109 is arranged at the movable end of the robotic arm. The robotic arm can drive the injection tube 109 to adjust its position to ensure that the bottom injection port of the injection tube 109 can be located at a position vertically opposite to the injection station 2023, thereby ensuring that the magnetic bead lysis solution can be accurately injected into the sample tube 3 vertically opposite to the injection station 2023.

[0085] Specifically, such as Figure 2 、 Figure 8 、 Figure 9 As shown, the robotic arm includes a mounting base 101, which is fixedly arranged on the top of the cracking turntable 2, and a liquid storage container 103 is arranged on the mounting base 101, and a vertically extending vertical pole 104 is provided on the mounting base 101, and a vertically movable lifting arm 105 is provided on the vertical pole 104, and a horizontal arm 107 extending in the horizontal direction is provided on the top of the lifting arm 105, and a pumping motor 108 and an injection pipe 109 are both arranged on the horizontal arm 107. By adjusting the lifting height of the lifting arm 105 on the vertical pole 104, the height of the horizontal arm 107 can be adjusted to achieve the purpose of adjusting the height of the injection pipe 109.

[0086] Further, such as Figure 2 、 Figure 8 、 Figure 9 As shown, a guide rail 1041 extending vertically is provided on the vertical pole 104, and the lifting arm 105 can be slidably connected to the guide rail 1041. A drive motor 102 is provided on the mounting base 101, and the driving shaft of the drive motor 102 is vertically upward and connected to the bottom end of the vertically arranged screw rod 106. The horizontal arm 107 is connected to the top end of the lifting arm 105, and a connecting block 1051 is provided at the bottom end of the lifting arm 105 (the lifting arm 105 and the connecting block 1051 can be integrally formed in an "L" shape), and a threaded hole is provided on the connecting block 1051, and the connecting block 1051 is screwed to the screw rod 106 through the threaded hole. During actual operation, the screw rod 106 is driven to rotate by the driving motor 102. Since the lifting arm 105 is screwed to the screw rod 106 through the connecting block 1051, and the lifting arm 105 is limited in the circumferential direction, when the screw rod 106 rotates, the lifting arm 105 can only move up or down along the screw rod 106 according to the rotation direction of the screw rod 106, so as to achieve the purpose of adjusting the height of the injection tube 109.

[0087] The working process of the lysis device for automated nucleic acid extraction of the present invention is as follows:

[0088] The sample tube 3 containing the sample to be lysed is placed in the sample initial hole 2013 vertically opposite the sample loading station 2025 at the sample loading station 2025. Then, the inner disk 2011 rotates clockwise, and the outer disk 2012 rotates counterclockwise synchronously. When the outer disk 2012 rotates to the next sample initial hole 2013 vertically opposite the sample loading station 2025, the next sample tube 3 containing the sample to be lysed can be placed. As the outer disk 2012 rotates, the placement of the sample tubes 3 is repeated in the same manner.

[0089] After the sample tube 3 containing the sample to be lysed is placed in the sample initial hole 2013 vertically opposite the sample loading station 2025, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to vertically opposite the cover opening station 2024, the inner disk 2011 and the outer disk 2012 stop rotating simultaneously, and the cover on the sample tube 3 is opened manually by a robot or a staff member.

[0090] After the lid on the sample tube 3 is opened, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to the first sample injection station 2021, the inner disk 2011 and the outer disk 2012 stop rotating simultaneously again. The sample tube 3 is transferred from the sample initial hole 2013 vertically opposite the first sample injection station 2021 to the sample processing hole 2014 vertically opposite the second sample injection station 2022 by a robot or manually by a staff member.

[0091] Afterwards, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. (When the sample processing hole 2014 where the sample tube 3 is located rotates clockwise with the inner disk 2011 to a position vertically opposite to the first sample outlet station 2029, the sample initial hole 2013 corresponding to the sample processing hole 2014 rotates counterclockwise with the outer disk 2012 to a position vertically opposite to the second sample outlet station 2028.) When the sample tube 3 rotates to a position vertically opposite to the injection station 2023, the drive motor 102 and the pumping motor 108 are simultaneously started to inject the magnetic bead lysis solution into the opened sample tube 3.

[0092] Afterwards, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to a position vertically aligned with the heating station 2026, the sample to be lysed and the magnetic bead lysis solution in the sample tube 3 are heated, thereby promoting the lysis of the sample to be lysed.

[0093] Afterwards, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to a position vertically opposite to the first sample output station 2029, the sample tube 3 is no longer heated. Simultaneously, the sample tube 3 is transferred from the sample processing hole 2014 vertically opposite to the first sample output station 2029 to the sample initial hole 2013 vertically opposite to the second sample output station 2028 by a robot or manually by a staff member.

[0094] Afterwards, the inner disk body 2011 and the outer disk body 2012 continue to rotate in opposite directions respectively. When the sample tube 3 rotates to a position vertically opposite to the capping and sample separation station 2027, the lid is manually covered on the top opening of the sample tube 3 by a robot or a staff member to complete the lysis operation of the sample to be lysed, and the sample tube 3 can be removed from the lysis turntable 2.

[0095] The characteristics and advantages of the lysis device for automated nucleic acid extraction of the present invention are:

[0096] First, this automated nucleic acid extraction lysis device allows for on-demand sample collection, accelerating the nucleic acid extraction lysis rate. It also eliminates the need to process samples in a timely manner even when large sample volumes are involved. The entire device is always ready to accept samples, eliminating the need to wait for samples to reach full capacity.

[0097] Second, the lysis device for automated nucleic acid extraction has a more streamlined structure, reduced volume, and lower cost. In addition, its streamlined structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has better use effect.

[0098] 3. The lysis device for automated nucleic acid extraction can automatically add magnetic bead lysis solution to the sample tube 3 vertically aligned therewith through the liquid injection mechanism 1, thereby greatly improving the degree of automation as well as the reliability and accuracy of the operation.

[0099] 4. In the lysis device for automated nucleic acid extraction, after adding the magnetic bead lysis solution to the sample tube 3, the sample tube 3 can be heated and the temperature can be stabilized within a range suitable for nucleic acid lysis, thereby increasing the nucleic acid lysis rate and improving the lysis effect.

[0100] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by those skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A lysis device for automated nucleic acid extraction, characterized in that: The lysis device for automated nucleic acid extraction comprises: A lysis turntable comprising an inner disk body and an outer disk body, the outer disk body being annularly arranged around the outer circumference of the inner disk body, the outer disk body being provided with a plurality of sample initial holes for accommodating sample tubes at intervals along its circumference, and the inner disk body being provided with a plurality of sample processing holes for accommodating the sample tubes at intervals along its circumference, wherein the plurality of sample initial holes correspond one-to-one with the plurality of sample processing holes in a radial direction of the lysis turntable, and the outer disk body and the inner disk body being rotatable in a first direction and a second direction, respectively, the first direction being opposite to the second direction; The lysis turntable further comprises a disk cover, the disk cover being located above the inner disk body and the outer disk body, and the disk cover covering the plurality of sample initial holes and the plurality of sample processing holes, the disk cover being sequentially provided with a second sample discharging station, a capping and sample separation station, a sample loading station, a cover opening station and a first sample loading station along the first direction, and the distances between the second sample discharging station, the capping and sample separation station, the sample loading station, the cover opening station and the first sample loading station and the central axis of the outer disk body are all equal to the distance between the sample initial holes and the central axis of the outer disk body; the disk cover being sequentially provided with a second sample loading station, a liquid injection station, a heating station and a first sample discharging station along the second direction, and the distances between the second sample loading station, the liquid injection station, the heating station and the first sample discharging station and the central axis of the inner disk body are all equal to the distance between the sample processing holes and the central axis of the inner disk body; The second sample discharging station, the capping and sample separation station, the sample loading station, the lid opening station, the first sample loading station, the second sample loading station, the liquid injection station, the heating station and the first sample discharging station are all through holes opened on the disk cover and are all located within the same semicircle of the disk cover; The first sample loading station and the second sample loading station are located on an extension line of the same radius of the disk cover, the second sample discharging station and the first sample discharging station are located on an extension line of the same radius of the disk cover, and positions of the first sample loading station and the second sample loading station on the disk cover are 180° apart from positions of the first sample discharging station and the second sample discharging station on the disk cover; The liquid injection mechanism includes a liquid injection tube for outputting magnetic bead lysis solution, and the liquid injection tube is located above the lysis turntable and vertically opposite to the liquid injection station.

2. The lysis device for automated nucleic acid extraction according to claim 1, wherein When the outer disk rotates along the first direction, the sample tube containing the sample to be lysed is placed from the sample loading station into any of the sample initial holes vertically opposite thereto, and the sample tube is uncapped when the sample tube rotates with the outer disk to be vertically opposite to the uncapping station; When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the first sampling station, the sample tube is transferred from the sample initial hole to any of the sample processing holes vertically opposite to the second sampling station.

3. The lysis device for automated nucleic acid extraction according to claim 2, wherein: When the sample tube rotates along the second direction with the inner disk to a position vertically opposite to the injection station, the magnetic bead lysis solution is injected into the sample tube through the injection tube, and the sample tube is heated at least when the sample tube rotates along the inner disk to a position vertically opposite to the heating station; When the sample tube rotates along the second direction with the inner disk to be vertically opposite to the first sample output station, the sample tube is transferred from the first sample output station to any of the sample initial holes vertically opposite to the second sample output station.

4. The lysis device for automated nucleic acid extraction according to claim 3, wherein: When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the capping and sample separation station, the capping and sample removal process is performed on the sample tube.

5. The lysis device for automated nucleic acid extraction according to claim 1, wherein: The cracking turntable further comprises a bottom plate with an opening at the top and a sealing at the bottom, wherein the bottom plate has a receiving space communicating with the top opening of the bottom plate, wherein the inner plate body and the outer plate body are rotatably disposed in the receiving space respectively, and a fixed shaft is fixed in the receiving space, wherein the inner plate body is disposed around the outer periphery of the fixed shaft. The liquid injection mechanism is arranged on the top of the disc cover; Alternatively, the disc cover is annular, and is arranged on the top opening of the base plate and is arranged around the outer circumference of the fixed shaft, and the liquid injection mechanism is arranged on the top of the fixed shaft.

6. The lysis device for automated nucleic acid extraction according to claim 5, wherein: The disk cover is provided with a plurality of first through holes spaced apart along its circumference, wherein the distance between the first through holes and the central axis of the outer disk body is equal to the distance between the sample initial hole and the central axis of the outer disk body, and the angle between two adjacent first through holes in the circumferential direction of the disk cover is equal to the angle between two adjacent sample initial holes in the circumferential direction of the outer disk body; The disk cover is provided with a plurality of second through holes spaced apart along its circumference, wherein the distance between the second through holes and the central axis of the inner disk body is equal to the distance between the sample processing hole and the central axis of the inner disk body, and the angle between two adjacent second through holes in the circumferential direction of the disk cover is equal to the angle between two adjacent sample processing holes in the circumferential direction of the inner disk body; The disc cover is provided with a plurality of hole position identification parts, and the plurality of hole position identification parts are used to mark the number and position of the plurality of first through holes and / or the plurality of second through holes.

7. The lysis device for automated nucleic acid extraction according to claim 1, wherein: The injection mechanism further includes a robotic arm and a liquid storage container, wherein the liquid outlet of the liquid storage container is connected to the injection tube via an infusion pipeline, and a pumping motor is provided on the infusion pipeline, and the pumping motor is used to pump the magnetic bead lysate stored in the liquid storage container to the injection tube; The fixed end of the robotic arm is connected to the lysis turntable, and the injection tube is arranged at the movable end of the robotic arm to adjust the position of the injection tube.

8. The lysis device for automated nucleic acid extraction according to claim 7, wherein: The robotic arm includes a mounting base, the liquid storage container is arranged on the mounting base, and the mounting base is provided with a vertical pole, a vertically movable lifting arm is provided on the vertical pole, a cross arm is provided on the top of the lifting arm, and the pumping motor and the injection pipe are both provided on the cross arm; A guide rail extending vertically is provided on the vertical pole, the lifting arm can be slidably connected to the guide rail, a driving motor is provided on the mounting base, the driving shaft of the driving motor is vertically upward and connected to the bottom end of the vertically arranged screw rod, the horizontal arm is connected to the top end of the lifting arm, and a connecting block is provided at the bottom end of the lifting arm, the connecting block has a threaded hole, and the connecting block is screwed to the screw rod through the threaded hole.

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