A method for culturing chicken myoblasts based on a parallel bioreactor

Abstract

This invention discloses a method for culturing chicken myoblasts based on a parallel bioreactor, belonging to the field of cell biology technology. The specific steps of the chicken myoblast culture method are: ① transferring a chicken myoblast suspension to a centrifuge tube and centrifuging it; ② after centrifugation, removing the supernatant from the centrifuge tube and resuspending the cells in fresh culture medium; ③ passage the chicken myoblasts; ④ inoculating them into a bioreactor, adding a mixture of microcarriers and culture medium, and continuing culture; ⑤ after completion, counting and harvesting the cells. The bioreactor used is a quadruple parallel stirred bioreactor, which precisely manages culture conditions, significantly reducing time and energy costs, thereby improving production efficiency and economic benefits. This invention, by combining microcarriers, serine, and fish skin collagen, significantly optimizes the culture environment of chicken myoblasts, ultimately significantly improving cell proliferation efficiency and yield, meeting the needs of large-scale production.

Description

Technical Field

[0001] This invention relates to the field of cell biology technology, specifically to a method for culturing chicken myoblasts based on a parallel bioreactor. Background Technology

[0002] Cultured meat, also known as lab-grown meat or clean meat, is an emerging technology developed to address the environmental and health problems caused by traditional meat production. Traditional livestock farming not only causes greenhouse gas emissions and depletes land resources but also raises animal welfare and food safety concerns. Cultured meat is produced by culturing animal cells in vitro, without involving slaughter, aiming to reduce environmental impact and improve food safety. Its production process includes four key steps: cell acquisition, development of serum-free culture media, large-scale cell expansion and differentiation, and food safety assessment.

[0003] In the technical solutions for using bioreactors for chicken myoblast culture, the existing technology has the following main drawbacks:

[0004] (1) Low cell expansion efficiency. Existing technologies exhibit low expansion efficiency of chicken myoblasts, resulting in slow cell proliferation, long production cycles, and insufficient yield. This drawback not only increases the time cost of research and production but also limits the feasibility of large-scale application. The low expansion efficiency primarily stems from the limitations of traditional two-dimensional culture methods. Furthermore, the uneven nutrient supply and waste removal in traditional culture methods lead to an unsatisfactory cell growth environment, further inhibiting cell proliferation.

[0005] (2) The operation is cumbersome and the time and economic costs are high. The existing technical solution requires multiple manual operations, which are cumbersome and prone to operational errors. Frequent operations not only consume a lot of manpower and time, but also significantly increase the overall cost and reduce production efficiency and economic benefits.

[0006] (3) Poor stability and consistency of the culture environment. In existing technologies, the parameters of the culture environment are not precisely controlled, resulting in unstable cell growth. The volatility and unpredictability of culture conditions lead to poor reproducibility of experimental results, affecting the quality and consistency of cell culture and limiting its potential for large-scale production. Summary of the Invention

[0007] The purpose of this invention is to provide a method for culturing chicken myoblasts based on a parallel bioreactor, in order to solve the problems of low cell expansion efficiency, cumbersome operation steps, high time and economic costs, and poor stability and consistency of the culture environment in the existing technology.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a method for culturing chicken myoblasts based on a parallel bioreactor.

[0009] The method for culturing chicken myoblasts based on a parallel bioreactor includes the following steps:

[0010] S1: Transfer the chicken myoblast cell suspension to a centrifuge tube and centrifuge it in a centrifuge.

[0011] S2: Remove the supernatant from the centrifuge tube and add fresh culture medium to resuspend the chicken myoblasts;

[0012] S3: The resuspended chicken myoblasts are seeded into a culture dish for expansion. Once the cells reach the target density, they are digested and the cells are counted.

[0013] S4: Digest and inoculate the chicken myoblasts described in S3 into the bioreactor, add the microcarrier and culture medium mixture, and adjust the bioreactor culture temperature, stirring speed, pH value and dissolved oxygen value, and culture for 7 days;

[0014] S5: After the culture is completed, stop stirring, let it stand and settle, remove the supernatant and digest the cells on the microcarrier with trypsin, count and harvest the cells;

[0015] In S4, the culture medium mixture includes culture medium, serine, and fish skin collagen.

[0016] Preferably, the chicken myoblast cell suspension in step S1 is transferred to a centrifuge tube, the centrifuge tube having a specification of 15 mL.

[0017] Preferably, the centrifugation conditions of the centrifuge in step S1 are a rotation speed of 1000 rpm and a centrifugation time of 3 min.

[0018] Preferably, the target density for chicken myoblast expansion in step S3 is 1×10⁻⁶. 5 per mL.

[0019] Preferably, the bioreactor in step S4 is a quadruple parallel stirred bioreactor.

[0020] Preferably, in step S4, the concentration of serine is maintained at 20–50 mg / mL throughout the entire culture process.

[0021] Preferably, in step S4, the concentration of the fish skin collagen is maintained at 80–120 mg / mL throughout the entire culture process.

[0022] Preferably, in step S4, the concentration of the microcarrier is maintained at 2 mg / mL throughout the entire culture process.

[0023] Preferably, in step S4, the culture temperature of the bioreactor is 37–41°C; the stirring speed of the bioreactor is 80–120 rpm; the pH value of the bioreactor is 7.2–7.4; and the dissolved oxygen value of the bioreactor is 40%–60%.

[0024] Preferably, in steps S2 and S4, the culture medium is DMEM medium containing 10% FBS.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] ① By employing a parallel bioreactor combined with additives such as microcarriers, serine, and fish skin collagen, this invention significantly optimizes the culture environment of chicken myoblasts, increases the cell growth surface area and three-dimensional culture space, ensures that cells obtain sufficient nutrition and attachment support, and ultimately significantly improves cell expansion efficiency and culture yield, meeting the needs of large-scale production.

[0027] ② This invention, through a parallel bioreactor system, reduces the cumbersome manual operation steps in traditional cultivation methods, lowers the possibility of operational errors, and significantly reduces time and energy costs, thereby improving production efficiency and economic benefits.

[0028] ③ This invention, through precise control of a parallel bioreactor, can monitor and adjust key parameters such as temperature, pH, dissolved oxygen, and stirring speed in real time during the culture process, ensuring the stability and consistency of the cell culture environment. This improves the reproducibility of experimental results and the quality of cell products, providing reliable technical support for industrial production. Attached Figure Description

[0029] Figure 1 This is a schematic flowchart of the chicken myoblast culture method according to Embodiment 2 of the present invention;

[0030] Figure 2 This is a flowchart illustrating the comparative example of the chicken myoblast culture method of the present invention.

[0031] Figure 3 These are cell adhesion rate diagrams for Examples 1, 2, 3, and 4 of the present invention;

[0032] Figure 4 These are cell proliferation curves for Examples 1, 2, 3, and 4 of the present invention;

[0033] Figure 5 These are cell adhesion rate diagrams for Examples 4, 5, and 6 of the present invention;

[0034] Figure 6 Cell proliferation curves for Examples 4, 5, and 6 of the present invention;

[0035] Figure 7 As a comparative example of the present invention, the cell adhesion rate of Example 4 and the control group without additives (without lysine and leucine) is shown in the figure.

[0036] Figure 8 As a comparative example of the present invention, the cell proliferation curves of Example 4 and the control group without additives (without lysine and leucine) are shown. Detailed Implementation

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods described in the following embodiments are conventional methods; unless otherwise specified, the reagents and materials can be obtained commercially.

[0038] In Examples 1-6 and the comparative examples below, the method for reviving chicken myoblasts was as follows: the chicken myoblast cryopreservation tubes were removed from liquid nitrogen and thawed in a 37°C water bath. After removal, the mouth of the cryopreservation tubes was wiped with alcohol. The cell suspension of chicken myoblasts was transferred to 15mL centrifuge tubes and centrifuged at 1000rpm for 3min. After centrifugation, the supernatant in the centrifuge tubes was aspirated, and the cells were resuspended in fresh culture medium. The culture medium used was DMEM medium containing 10% FBS.

[0039] Example 1

[0040] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0041] Step 2: Add 1×10 7 Cells were seeded into parallel bioreactors (quadruple parallel stirred bioreactors), with microcarriers and culture medium added. The culture medium was DMEM containing 10% FBS. The culture medium volume was consistently 100 mL, and the concentration of the microcarriers was maintained at 2 mg / mL throughout the culture process. The bioreactor culture temperature was precisely controlled at 37°C, the stirring speed at 120 rpm, the pH at 7.2, and the dissolved oxygen level at 60% to ensure a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed every other day. The culture process lasted for 7 days, with all parameters monitored in real time to ensure optimal cell growth.

[0042] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0043] Example 2

[0044] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0045] Step 2: Add 1×10 7 Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with a mixture of microcarriers and culture medium added, the volume of which was always 100 mL. The culture medium mixture included serine and DMEM containing 10% FBS. The concentration of microcarriers was maintained at 2 mg / mL throughout the culture, and the concentration of serine was maintained at 30 mg / mL to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 37°C, the stirring speed at 120 rpm, the pH at 7.2, and the dissolved oxygen level at 60%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with all parameters monitored in real time to ensure optimal cell growth.

[0046] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0047] Example 3

[0048] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0049] Step 2: Add 1×10 7Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with microcarriers and culture medium mixed, the volume of which was always 100 mL. The culture medium mixture included fish skin collagen and culture medium. The culture medium was DMEM medium containing 10% FBS. The concentration of the microcarriers was maintained at 2 mg / mL throughout the culture process. The concentration of fish skin collagen was maintained at 100 mg / mL throughout the culture process to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 37°C, the stirring speed at 120 rpm, the pH at 7.2, and the dissolved oxygen level at 60%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with real-time monitoring of various parameters to ensure that the cells grew under optimal conditions.

[0050] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0051] Example 4

[0052] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0053] Step 2: Add 1×10 7Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with microcarriers and culture medium mixed, the volume of which was always 100 mL. The culture medium mixture included serine, fish skin collagen, and DMEM medium containing 10% FBS. The concentration of microcarriers was maintained at 2 mg / mL throughout the culture. The concentration of serine was maintained at 30 mg / mL throughout the culture. The concentration of fish skin collagen was maintained at 100 mg / mL throughout the culture to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 37°C, the stirring speed at 120 rpm, the pH at 7.2, and the dissolved oxygen level at 60%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with all parameters monitored in real time to ensure that the cells grew under optimal conditions.

[0054] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0055] Example 5

[0056] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0057] Step 2: Add 1×10 7Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with microcarriers and culture medium mixed, the volume of which was always 100 mL. The culture medium mixture included serine, fish skin collagen, and DMEM medium containing 10% FBS. The concentration of microcarriers was maintained at 2 mg / mL throughout the culture. The concentration of serine was maintained at 20 mg / mL throughout the culture. The concentration of fish skin collagen was maintained at 120 mg / mL throughout the culture to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 41°C, the stirring speed at 100 rpm, the pH at 7.4, and the dissolved oxygen level at 40%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with all parameters monitored in real time to ensure that the cells grew under optimal conditions.

[0058] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0059] Example 6

[0060] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cells reach a suitable density of 1×10⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0061] Step 2: Add 1×10 7Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with microcarriers and culture medium mixed, the volume of which was always 100 mL. The culture medium mixture included serine, fish skin collagen, and DMEM medium containing 10% FBS. The concentration of microcarriers was maintained at 2 mg / mL throughout the culture process. The concentration of serine was maintained at 50 mg / mL throughout the culture process. The concentration of fish skin collagen was maintained at 80 mg / mL throughout the culture process to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 38°C, the stirring speed at 80 rpm, the pH at 7.3, and the dissolved oxygen level at 50%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with real-time monitoring of various parameters to ensure that the cells grew under optimal conditions.

[0062] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0063] Comparative Example

[0064] Step 1: Inoculate the revived chicken myoblasts into culture dishes for expansion until the cell density reaches an appropriate level of 1×10⁻⁶. 5 After the cells were counted at a rate of 1 per mL, they were digested and counted.

[0065] Step 2: Add 1×10 7Cells were seeded into a parallel bioreactor (a quadruple parallel stirred bioreactor), with microcarriers and culture medium mixed, the volume of which was always 100 mL. The culture medium mixture included lysine, leucine, and DMEM containing 10% FBS. The concentration of the microcarriers was maintained at 2 mg / mL throughout the culture. The concentrations of lysine and leucine were maintained at 1 mg / mL and 0.5 mg / mL throughout the culture to provide a larger surface area, abundant nutrients, and a suitable extracellular matrix environment. The culture volume in the bioreactor was strictly maintained to ensure stable concentrations of the additives. The bioreactor culture temperature was precisely controlled at 37°C, the stirring speed at 120 rpm, the pH at 7.2, and the dissolved oxygen level at 60%, ensuring a stable and consistent culture environment. Cell adhesion was assessed after 24 hours of culture, and cell proliferation was assessed the following day. The culture process lasted for 7 days, with real-time monitoring of all parameters to ensure optimal cell growth.

[0066] Step 3: After culturing, stop stirring, allow to settle, remove the supernatant, and digest the cells on the microcarriers with trypsin. Finally, count and harvest the cells.

[0067] Figure 1 This is a flowchart illustrating the chicken myoblast culture method of Example 2. Figure 2 This is a flowchart illustrating a comparative method for culturing chicken myoblasts.

[0068] Results and Discussion

[0069] (1) Figure 3 These are cell adhesion rate graphs from Examples 1, 2, 3, and 4 of this invention. The study investigated the effects of adding serine, fish skin collagen, and simultaneously adding both substances on cell adhesion rate. Compared to Example 1 without any additives, the addition of serine and fish skin collagen significantly improved cell adhesion rate. This is because serine actively promotes the synthesis of extracellular matrix and cell surface proteins, while fish skin collagen provides more adhesion sites for cells. When both are used simultaneously, the cell adhesion rate is further enhanced, demonstrating a synergistic effect.

[0070] (2) Figure 4 These are cell proliferation curves from Examples 1, 2, 3, and 4 of the present invention. Figure 4This study demonstrates the effects of adding serine, fish skin collagen, and both simultaneously on cell proliferation efficiency during chicken myoblast culture in parallel bioreactors in Examples 1, 2, 3, and 4. Compared to Example 1 without any additives, the addition of 30 mg / mL serine or 100 mg / mL fish skin collagen significantly improved cell proliferation efficiency. The cell proliferation effect was even more pronounced with the addition of both serine and fish skin collagen. This increased proliferation efficiency is primarily attributed to the fact that serine and fish skin collagen provide cells with additional surface area, nutrients, and an extracellular matrix environment, which facilitates cell adhesion and proliferation.

[0071] (3) Figure 5 These are cell adhesion rate diagrams for Examples 4, 5, and 6 of the present invention. Figure 5 The results of chicken myoblast cultured in parallel bioreactors in Examples 4, 5, and 6 are presented. Each experiment was conducted under different culture conditions, with varying concentrations of serine and fish skin collagen added, and the culture environment parameters were adjusted to examine the effects of different combinations of conditions on chicken myoblast adhesion efficiency. The results showed that despite differences in the concentrations of serine and fish skin collagen, the cell adhesion rates among the groups were not significantly different, indicating that within the tested concentration range, the addition of these two substances had a limited impact on cell adhesion.

[0072] (4) Figure 6 These are cell adhesion rate diagrams for Examples 4, 5, and 6 of the present invention. Figure 6 Examples 4, 5, and 6 demonstrate the use of parallel bioreactors for culturing chicken myoblasts, with different concentrations of serine and fish skin collagen added simultaneously, and the reactors operated under different culture conditions. The experimental results show that adjusting the concentrations of serine and fish skin collagen, or changing specific culture parameters (such as temperature, stirring rate, and dissolved oxygen concentration), did not significantly affect cell proliferation in the three examples. Although adjustments to concentrations and conditions did affect the cell microenvironment, the final difference in cell proliferation was small. This indicates that, within a certain range, changes in the concentrations of serine and fish skin collagen and culture conditions do not significantly affect the proliferation of chicken myoblasts, suggesting that these conditions are sufficient to meet the cell proliferation requirements.

[0073] (5) Figure 7 This is a comparative example of the present invention, Example 4, showing the cell adhesion rate compared to the control group without additives (no lysine and leucine added). Figure 7The results showed that the addition of lysine and leucine in the comparative example significantly improved cell adhesion, exhibiting higher adhesion efficiency compared to the control group cells without additives. This indicates that lysine and leucine not only provide cells with the necessary amino acid supplementation but may also promote cell-microcarrier adhesion by improving the extracellular matrix environment or enhancing cell membrane adhesive properties. However, compared to Example 4, the cell adhesion rate was still lower, and therefore it was considered an alternative. In contrast, the effect of lysine and leucine was not as good as the combination of serine and fish skin collagen, which showed better performance in cell adhesion rate because the combination of serine and fish skin collagen better mimics the extracellular matrix and promotes cell adhesion, providing a more favorable adhesion environment and signal transduction support. The control group in this example did not contain added lysine and leucine, but all other conditions were the same.

[0074] (6) Figure 8 This is a comparative example of the present invention, Example 4, and the cell proliferation curve of the control group without additives (no lysine and leucine added). Figure 8 The results showed that the addition of lysine and leucine significantly enhanced cell proliferation. Experimental data indicated that the control group with these two amino acids showed significantly better proliferation rate and total cell number than the control group without them. Lysine and leucine not only participate in protein synthesis as essential amino acids but also play important roles in cell metabolism and signal transduction, thereby promoting cell cycle progression and accelerating cell division and growth. Furthermore, these two amino acids may enhance the cell's responsiveness to external stimuli by providing a better nutritional environment, thus improving the overall culture effect. The control group in this study did not contain lysine and leucine, but all other conditions were the same.

[0075] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for culturing chicken myoblasts based on a parallel bioreactor, characterized in that: The method for culturing chicken myoblasts based on a parallel bioreactor includes the following steps: S1: Transfer the chicken myoblast cell suspension to a centrifuge tube and centrifuge it in a centrifuge. S2: Remove the supernatant from the centrifuge tube and add fresh culture medium to resuspend the chicken myoblasts; S3: The resuspended chicken myoblasts are seeded into a culture dish for expansion. Once the cells reach the target density, they are digested and the cells are counted. S4: Digest and inoculate the chicken myoblasts described in S3 into the bioreactor, add the microcarrier and culture medium mixture, and adjust the bioreactor culture temperature, stirring speed, pH value and dissolved oxygen value, and culture for 7 days; S5: After the culture is completed, stop stirring, let it stand and settle, remove the supernatant and digest the cells on the microcarrier with trypsin, count and harvest the cells; In S4, the culture medium mixture includes culture medium, serine, and fish skin collagen; The concentration of serine was maintained at 20–50 mg / mL throughout the entire culture process; The concentration of the fish skin collagen was maintained at 80–120 mg / mL throughout the entire culture process; In step S4, the concentration of the microcarrier is maintained at 2 mg / mL throughout the entire culture process; The bioreactor in step S4 is a quadruple parallel stirred bioreactor; In step S4, the culture temperature of the bioreactor is 37–41°C; the stirring speed of the bioreactor is 80–120 rpm; the pH value of the bioreactor is 7.2–7.4; and the dissolved oxygen value of the bioreactor is 40%–60%.

2. The method for culturing chicken myoblasts based on a parallel bioreactor according to claim 1, characterized in that: In step S1, the cell suspension of chicken myoblasts is transferred to a centrifuge tube, the centrifuge tube being 15 mL in size.

3. The method for culturing chicken myoblasts based on a parallel bioreactor according to claim 1, characterized in that: The centrifugation conditions of the centrifuge in step S1 are a rotation speed of 1000 rpm and a centrifugation time of 3 min.

4. The method for culturing chicken myoblasts based on a parallel bioreactor according to claim 1, characterized in that: The target density for chicken myoblast expansion in step S3 is 1×10⁻⁶. 5 per mL.

5. The method for culturing chicken myoblasts based on a parallel bioreactor according to claim 1, characterized in that: In steps S2 and S4, the culture medium is DMEM medium containing 10% FBS.

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