Evaluation method and device for characterizing curative effect of anti-cancer drug based on optical lever cell mechanics

Abstract

The invention relates to the field of biological medicine, and discloses an evaluation device for characterizing the curative effect of an anti-cancer drug based on optical lever cell mechanics, the evaluation device comprises an objective table and a light source, a micro-motion adjusting device is arranged above the objective table, a force detector is installed on the micro-motion adjusting device, the light source is focused on the force detector through an optical lens, and the optical lens is arranged on the objective table. The force detector reflects light to a light detection sensor, the data feedback transmission end of the light detection sensor is connected with the data input end of a data analysis recognizer, and the data output end of the data analysis recognizer is connected with the output and input end of a data control analysis system. The data control and analysis system controls the micro-motion adjusting device through programming, and on the basis of the optical principle, namely light refraction analysis, the original object surface appearance is constructed to achieve detection of cell membrane damage, so that the damage degree of a drug to a cancer cell membrane is judged, namely, the effectiveness of the drug to cancer cells is evaluated.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine, and more specifically, to an evaluation method and device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics. Background Art

[0002] Prostate cancer is a malignant tumor with a very high incidence rate. It ranks second in the incidence rate of male malignant tumors worldwide, second only to lung cancer. Although there are currently a variety of therapeutic drugs for it, the effectiveness and safety of drugs still require more complete evaluation methods. With the development of nanotechnology, it has become possible to use micro-motion technology combined with optical detection technology to detect changes in cell morphology after drug administration.

[0003] The present invention provides an evaluation method and device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics. The evaluation method mainly uses a self-made cell mechanics measurement device to characterize the ultra-micro morphological changes of the cell surface of prostate cancer cells before and after paclitaxel treatment, and obtains the three-dimensional morphology of cancer cells based on the optical lever force measurement principle and feedback information. Among them, the present invention is mainly based on the fact that the cancer cell membrane will be torn open after being acted upon by anticancer drugs. Therefore, the present invention uses the nanoscale holes as an important characterization for evaluating drug efficacy, and can accurately observe the subtle characteristics of cancer cells under drug stimulation. With the help of the hole structure, the killing effect of anticancer drugs on cancer cells can be visualized, providing a new method for visual evaluation of the efficacy of new anti-tumor drugs. Summary of the invention

[0004] The purpose of the present invention is to address the deficiencies in the prior art and to provide an evaluation method and device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics to solve the above problems.

[0005] The above technical purpose of the present invention is achieved through the following technical solutions: an evaluation device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics, comprising a stage and a light source, a micro-adjustment device is arranged above the stage, a force detector is installed on the micro-adjustment device, the light source is focused onto the force detector through an optical lens, the force detector reflects light to a light detection sensor, the data feedback transmission end of the light detection sensor is connected to the data input end of a data analysis identifier, the data output end of the data analysis identifier is connected to the output input end of a data control and analysis system, and the data control and analysis system controls the micro-adjustment device through programming.

[0006] Furthermore, the micro-adjustment device includes a piezoelectric brake, and the force detector includes a cantilever beam with a probe.

[0007] Furthermore, the micro-adjustment device includes movement in three directions: X / Y / Z.

[0008] A method for evaluating the efficacy of anticancer drugs based on optical lever cell mechanics, including determining the effect of anticancer drugs by detecting the results of the torn holes in the cancer cell membrane after the anticancer drugs act on it.

[0009] Follow these steps:

[0010] Step 1: Place the sample to be tested in a constant temperature incubator and culture it until the cell density reaches a predetermined percentage;

[0011] Step 2: pre-treating the sample to be tested that has been cultured to reach the cell sealing percentage in step 1;

[0012] Step 3: Wash the cells obtained in step 2 with PBS13, and then fix the cells, i.e., fix them on the stage.

[0013] Step 4, the cells fixed in step 3 are subjected to morphological detection by an evaluation device based on optical lever cell mechanics to characterize the efficacy of anticancer drugs;

[0014] Step five, performing morphology analysis on the morphology detection data of step four.

[0015] Furthermore, the pretreatment includes adding drugs of different concentrations for incubation for a certain period of time.

[0016] Furthermore, the inner and outer loops of the coil are wound in a double helix.

[0017] According to the above scheme,

[0018] Compared with the prior art, the beneficial effects are as follows:

[0019] The present invention is based on optical principles, that is, light refraction analysis to construct the original surface morphology of an object to detect cell membrane damage, thereby determining the degree of damage to the cancer cell membrane caused by the drug, that is, to evaluate the effect of the drug on cancer cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a diagram of the device of the present invention;

[0021] Figure 2 The figure is a flow chart of the method of the present invention.

[0022] In the figure, 1 is the loading platform; 2 is the sample to be tested; 3 is the light detection sensor; 4 is the force detector; 5 is the micro-adjustment device; 6 is the light source; 7 is the data analysis identifier; and 8 is the data control and analysis system. DETAILED DESCRIPTION

[0023] The invention will be further described in detail below with reference to the accompanying drawings and through specific embodiments. The following embodiments are merely illustrative and are not intended to limit the scope of protection of the present invention.

[0024] Example 1

[0025] like Figure 1 As shown: An evaluation device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics, comprising a stage 1 and a light source 6, a micro-adjustment device 5 is arranged above the stage 1, a force detector 4 is installed on the micro-adjustment device 5, the light source 6 is focused on the force detector 4 through an optical lens, the force detector 4 reflects light to the light detection sensor 3, the data feedback transmission end of the light detection sensor 3 is connected to the data input end of a data analysis identifier 7, the data output end of the data analysis identifier 7 is connected to the output input end of a data control analysis system 8, and the data control analysis system 8 controls the micro-adjustment device 5 through programming.

[0026] The micro-adjustment device 5 includes a piezoelectric brake, and the force detector 4 includes a cantilever beam with a probe.

[0027] The micro-adjustment device 5 includes movement in three directions: X / Y / Z.

[0028] The above structure is explained as follows: the sample 2 to be tested is fixed on the stage 1; the force detector 4 is a structure with a cantilever beam and a tip, and is fixed on the micro-adjustment device 5; the micro-adjustment device 5 can realize movement in three directions of X / Y / Z. In order to realize the control of micro-nano size, the micro-adjustment device 5 can use piezoelectric brakes, etc.

[0029] The main implementation principle is that when the light source 6 is focused on the force detector 4 through the optical lens, the light will be reflected to the light detector sensor 3. When the surface of the sample 2 under test is undulating, according to the principle of the optical lever, the focus point on the light detector sensor 3 will fluctuate. According to this principle, the optical signal generated on the light detector sensor 3 is converted into an electrical signal, and transmitted to the data analysis identifier 7 as an actual value. The data control and analysis system 8 sets the initial value and sends two signals to the micro-adjustment device 5 and the data analysis identifier 7 respectively. The mobile platform moves in three directions according to the data of the data control and analysis system 8, and the data analysis identifier 7 makes judgments and corrections based on the initial value and the actual value transmitted from the light detector sensor 3, and feeds back the final result to the data control and analysis system 8 to form a closed-loop control, and the morphological image is displayed on the data control and analysis system 8.

[0030] A method for evaluating the efficacy of anticancer drugs based on optical lever cell mechanics, including determining the effect of anticancer drugs by detecting the results of the torn holes in the cancer cell membrane after the anticancer drugs act on it.

[0031] Follow these steps:

[0032] Step 1: Place the sample to be tested in a constant temperature incubator and culture it until the cell density reaches a predetermined percentage;

[0033] Step 2: pre-treating the sample to be tested that has been cultured to reach the cell sealing percentage in step 1;

[0034] Step 3: Wash the cells obtained in step 2 with PBS13, and then fix the cells, i.e., fix them on the stage (1).

[0035] Step 4, the cells fixed in step 3 are subjected to morphological detection by an evaluation device based on optical lever cell mechanics to characterize the efficacy of anticancer drugs;

[0036] Step five, performing morphology analysis on the morphology detection data of step four.

[0037] Furthermore, the pretreatment includes adding drugs of different concentrations for incubation for a certain period of time.

[0038] The method proposed in the present invention is mainly based on the fact that the cancer cell membrane will be torn open after being acted upon by anticancer drugs. Figure 2 As shown, the sample 2 to be tested is placed in a constant temperature incubator for culture 11. When the cell density reaches a predetermined percentage, pretreatment 12 is performed, that is, drugs of different concentrations are added for incubation for a certain period of time. After the incubation is completed, PBS 13 is used for washing, and then the cells are fixed 14. Then, the micro-force measuring device of the present invention is used to perform morphology detection 15, and finally, the morphology analysis is performed according to the different detection results 16. The present invention uses the nanoscale holes as an important representation for evaluating drug efficacy, and can accurately observe the subtle characteristics of cancer cells under drug stimulation. With the help of the hole structure, the killing effect of anticancer drugs on cancer cells can be visualized, which provides a new method for visual evaluation of the efficacy of new anti-tumor drugs.

[0039] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. An evaluation device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics, characterized by: The invention comprises an object platform (1) and a light source (6), wherein a micro-adjustment device (5) is arranged above the object platform (1), a force detector (4) is installed on the micro-adjustment device (5), the light source (6) is focused onto the force detector (4) through an optical lens, the force detector (4) reflects light to a light detection sensor (3), the data feedback transmission end of the light detection sensor (3) is connected to the data input end of a data analysis identifier (7), the data output end of the data analysis identifier (7) is connected to the output input end of a data control and analysis system (8), and the data control and analysis system (8) controls the micro-adjustment device (5) through programming.

2. According to claim 1, the device for evaluating the efficacy of anticancer drugs based on optical lever cell mechanics is characterized by: The micro-adjustment device (5) comprises a piezoelectric brake, and the force detector (4) comprises a cantilever beam with a probe.

3. The evaluation device for characterizing the efficacy of anticancer drugs based on optical lever cell mechanics according to claim 2, characterized in that: The micro-adjustment device (5) includes movement in three directions: X / Y / Z.

4. A method for evaluating the efficacy of anticancer drugs based on optical lever cell mechanics, characterized in that: This includes determining the effect of anticancer drugs by detecting the results of holes in the cancer cell membrane after being torn by anticancer drugs. Follow these steps: Step 1: Place the sample to be tested in a constant temperature incubator and culture it until the cell density reaches a predetermined percentage; Step 2: pre-treating the sample to be tested that has been cultured to reach the cell sealing percentage in step 1; Step 3: Wash the cells obtained in step 2 with PBS13, and then fix the cells, i.e., fix them on the stage (1). Step 4, the cells fixed in step 3 are subjected to morphological detection by an evaluation device based on optical lever cell mechanics to characterize the efficacy of anticancer drugs; Step five, performing morphology analysis on the morphology detection data of step four.

5. The method for evaluating the efficacy of anticancer drugs based on optical lever cell mechanics according to claim 4, characterized in that: The pretreatment includes adding drugs of different concentrations and incubating for a certain period of time.

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