Kitchen paper with broad-spectrum bactericidal performance and preparation method thereof

Abstract

The invention provides kitchen paper with broad-spectrum bactericidal performance and a preparation method thereof, and belongs to the technical field of paper for daily use. The preparation method of the kitchen paper with the broad-spectrum bactericidal performance comprises the following steps: mixing cellulose nanocrystals, nisin and water under an acidic condition to obtain a nano-composite solution; and spraying the nano-composite solution to the surface of a formed handsheet to obtain the kitchen paper with the broad-spectrum bactericidal performance. The kitchen paper prepared by the preparation method disclosed by the invention has a bactericidal effect, nisin in the kitchen paper is a natural bioactive antibacterial peptide, is a pure natural, efficient and safe polypeptide active substance extracted by utilizing a biotechnology, has a very good inhibition and bactericidal effect on gram positive bacteria such as staphylococcus aureus and bacillus, and can be used for preparing the kitchen paper. By applying the kitchen paper, the decay of meat can be obviously slowed down.

Description

Technical Field

[0001] The invention belongs to the technical field of household paper, and in particular relates to kitchen paper with broad-spectrum bactericidal performance and a preparation method thereof. Background Art

[0002] Kitchen paper is inseparable from people's lives. In recent years, with the improvement of living standards, people have not only become more aware of the use of kitchen paper, but also paid more and more attention to its quality and safety.

[0003] Currently, kitchen paper on the market is mostly used to absorb water and oil, and is expensive and has a complex formula. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a kitchen paper with broad-spectrum bactericidal performance and a preparation method thereof. The preparation method provided by the present invention has a simple formula, is easy to operate, and has low cost, and the prepared kitchen paper has broad-spectrum bactericidal performance.

[0005] In order to achieve the above object, the present invention provides the following scheme:

[0006] The present invention provides a method for preparing kitchen paper with broad-spectrum bactericidal performance, comprising the following steps:

[0007] Under acidic conditions, cellulose nanocrystals, nisin and water are mixed to obtain a nanocomposite solution;

[0008] The nanocomposite solution is sprayed onto the surface of the formed hand-sheet to obtain the kitchen paper with broad-spectrum bactericidal performance.

[0009] Preferably, the mixing of cellulose nanocrystals, nisin and water under acidic conditions comprises: mixing cellulose nanocrystals and water, then adjusting the pH value of the resulting suspension to be acidic, and then adding nisin.

[0010] Preferably, the pH value of the acidic condition is 4-5.

[0011] Preferably, the mass of the cellulose nanocrystals is 0.06% of the total mass of the cellulose nanocrystals and water.

[0012] Preferably, the mass concentration of nisin in the nanocomposite solution is 0.01-0.03%.

[0013] Preferably, the spraying amount of the nanocomposite solution is 600-700 mL / m 2 Handwritten piece.

[0014] Preferably, the spraying angle is 60 to 90°, and the spraying distance is 15 to 20 cm.

[0015] Preferably, the weight of the hand sheet is 40 to 50 g / m 2 .

[0016] Preferably, the preparation of the formed handsheet comprises the following steps:

[0017] Soaking the bleached bamboo pulp board in the first water, tearing the obtained pulp board into shredded paper pieces, then adding the second water, performing the first decomposition, drying the water, and then breaking up to obtain pulp;

[0018] A third water is added to the slurry for beating, and then the slurry is secondarily deflated into single fibers, and then paper-made to obtain a formed hand sheet.

[0019] The present invention provides kitchen paper with broad-spectrum bactericidal performance prepared by the preparation method described in the above scheme.

[0020] The invention provides a method for preparing kitchen paper with broad-spectrum bactericidal performance, comprising the following steps: mixing cellulose nanocrystals, nisin and water under acidic conditions to obtain a nanocomposite solution; spraying the nanocomposite solution onto the surface of a formed hand-sheet to obtain the kitchen paper with broad-spectrum bactericidal performance.

[0021] The kitchen paper prepared by the present invention has a bactericidal effect, wherein Nisin is a natural biologically active antimicrobial peptide, which is a pure natural, efficient and safe polypeptide active substance extracted by biotechnology, and has a good inhibitory and bactericidal effect on Gram-positive bacteria, such as Staphylococcus aureus and Bacillus, etc. The use of the kitchen paper of the present invention can significantly slow down the corruption of meat. For kitchen paper, the environment in which it is used is to wipe water stains and oil stains on the surface of tableware and meat in the kitchen, and it is of great significance to effectively inhibit bacteria or pathogens (Gram-positive bacteria, such as Staphylococcus aureus, Bacillus, etc.) that easily cause meat corruption in the kitchen environment.

[0022] The cellulose nanocrystals (CNC) used in the present invention have a high specific surface area and can be used as a green adsorption carrier of Nisin; at the same time, there are a large number of free hydroxyl groups on the surface of CNC, which can form hydrogen bonds with paper fibers to increase the retention rate of Nisin on kitchen paper, thereby improving the antibacterial effect of kitchen paper.

[0023] Furthermore, the raw material used in the present invention is bleached bamboo pulp, which itself has a certain antibacterial effect. The method adopted is a process of spraying and drying on the paper surface, which can lock Nisin on the surface of kitchen paper well, providing antibacterial and bactericidal effects for the kitchen paper and improving the consumer experience.

[0024] The preparation method provided by the invention has simple formula, is easy to operate and has low cost. DETAILED DESCRIPTION

[0025] The present invention provides a method for preparing kitchen paper with broad-spectrum bactericidal performance, comprising the following steps:

[0026] Under acidic conditions, cellulose nanocrystals, nisin and water are mixed to obtain a nanocomposite solution;

[0027] The nanocomposite solution is sprayed onto the surface of the formed hand-sheet to obtain the kitchen paper with broad-spectrum bactericidal performance.

[0028] Unless otherwise specified, the materials and equipment used in the present invention are commercially available.

[0029] The invention mixes cellulose nanocrystals, nisin and water under acidic conditions to obtain a nanocomposite solution.

[0030] In the present invention, the mixing of cellulose nanocrystals, nisin and water under acidic conditions preferably comprises: mixing cellulose nanocrystals and water, then adjusting the pH value of the resulting suspension to be acidic, and then adding nisin.

[0031] In the present invention, the pH value of the acidic condition is preferably 4 to 5, and more preferably 5; the pH value is preferably adjusted using a hydrochloric acid solution; and the concentration of the hydrochloric acid solution is preferably 12 mol / L.

[0032] In the present invention, the mass of the cellulose nanocrystals is preferably 0.06% of the total mass of the cellulose nanocrystals and water; the mass concentration of nisin in the nanocomposite solution is preferably 0.01-0.03%, and more preferably 0.015-0.025%.

[0033] After obtaining the nanocomposite solution, the present invention sprays the nanocomposite solution onto the surface of the formed hand-sheet to obtain the kitchen paper with broad-spectrum bactericidal performance.

[0034] In the present invention, the weight of the hand sheet is preferably 40 to 50 g / m 2 , more preferably 42 to 48 g / m 2 In the present invention, the preparation of the formed hand sheet preferably comprises the following steps:

[0035] Soaking the bleached bamboo pulp board in the first water, tearing the obtained pulp board into shredded paper pieces, then adding the second water, performing the first decomposition, drying the water, and then breaking up to obtain pulp;

[0036] A third water is added to the slurry for beating, and then the slurry is secondarily deflated into single fibers, and then paper-made to obtain a formed hand sheet.

[0037] In the present invention, the mass ratio of the bleached bamboo pulp board to the volume of the first water is preferably 360g:5L; the mass ratio of the bleached bamboo pulp board to the volume of the second water is preferably 360g:18L; the temperature of the second water is preferably 20°C.

[0038] In the present invention, the first deflaking time is preferably 5 to 8 minutes. In the present invention, the first deflaking is preferably performed until there are no small pulp lumps.

[0039] After the breaking up, the present invention preferably puts the obtained slurry into a sealed bag, and then puts it into a refrigerator to even out the moisture, and then refrigerates it for later use after measuring the moisture content. The present invention can calculate the mass of the required slurry by quantitatively measuring the hand sheet by measuring the moisture content.

[0040] In the present invention, the amount of the third water, calculated on the basis of absolute dry pulp, is preferably used to adjust the pulp mass concentration to 10%.

[0041] In the present invention, the beating is preferably performed to a beating degree of 30 to 40°SR, more preferably 32 to 38°SR. In the present invention, the number of revolutions of the second deflation is preferably 7000r.

[0042] In the present invention, the spraying amount of the nanocomposite solution is preferably 600-700 mL / m 2 Hand sheet, more preferably 620~680mL / m 2 Handsheet. In the present invention, the spraying angle is preferably 60-90°, more preferably 65-85°, i.e., the angle between the spraying device (spray gun muzzle) and the handsheet; the spraying distance is preferably 15-20 cm, more preferably 16-18 cm, i.e., the distance between the spraying device and the formed handsheet.

[0043] After the spraying is completed, the present invention preferably performs drying on the formed hand sheet; the drying temperature is preferably 95°C.

[0044] The raw material used in the present invention is bleached bamboo pulp, which itself has a certain antibacterial effect. The method adopted is a process of spraying and drying on the paper surface, which can well lock Nisin on the surface of kitchen paper, provide antibacterial and bactericidal effects for the kitchen paper, and improve the consumer experience.

[0045] The cellulose nanocrystals (CNC) used in the present invention have a high specific surface area and can be used as a green adsorption carrier of Nisin; at the same time, there are a large number of free hydroxyl groups on the surface of CNC, which can form hydrogen bonds with paper fibers to increase the retention rate of Nisin on kitchen paper, thereby improving the antibacterial effect of kitchen paper.

[0046] The present invention provides kitchen paper with broad-spectrum bactericidal performance prepared by the preparation method described in the above scheme.

[0047] The kitchen paper prepared by the present invention has a bactericidal effect. Nisin therein is a natural biologically active antimicrobial peptide, which is a pure natural, efficient and safe polypeptide active substance extracted by biotechnology. It has a good inhibitory and bactericidal effect on Gram-positive bacteria, such as Staphylococcus aureus and Bacillus, etc. The use of the kitchen paper of the present invention can significantly slow down the corruption of meat.

[0048] In order to further illustrate the present invention, a kitchen paper with broad-spectrum bactericidal performance and a preparation method thereof provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0049] The base paper (i.e., hand sheets) of each embodiment was made by a rapid paper sheet former, CNC was purchased from Zhejiang Jinjiahao Nanotechnology Co., Ltd., and Nisin was purchased from the McLean reagent platform.

[0050] Example 1

[0051] A method for preparing kitchen paper with broad-spectrum bactericidal performance is as follows:

[0052] CNC and deionized water were mixed to prepare a CNC suspension with a mass concentration of 0.06%, and hydrochloric acid with a concentration of 12 mol / L was added to adjust the pH value of the CNC suspension to 5. The CNC suspension with the adjusted pH value was used as a solvent, and Nisin powder was added thereto. The amount of Nisin added was controlled so that the mass concentration of Nisin in the obtained Nisin-CNC nanocomposite solution was 0.01%, and the prepared solution was placed in a spraying device;

[0053] Weigh 360g of bleached bamboo pulp board, soak the pulp board with 5L of water for more than 4h, and then tear the obtained pulp board into small pieces of about 4cm×4cm for beating; add 18L of deionized water with a temperature of 20℃ into the pulp tank of the Wally beater, start the beater, slowly add the pulp into the beater, control the decomposition time to 5-8min, and decompose until there are no small pulp lumps; take out the decomposition pulp, shake dry the water, break it up and put it into a sealed bag, put it in a refrigerator to evenly distribute the water content, and then refrigerate it for later use;

[0054] The refrigerated pulp is refined by PFI to 30-40°SR, and then deflaked by a deflaker at 7000r until the pulp is completely dispersed into single fibers. The paper is then made into several sheets with a basis weight of 40g / m2 by a fast sheet former. 2 The hand-copied sheet (area 3.14×10 - 2 m 2 ), to obtain a formed hand sheet;

[0055] After the hand sheet was formed, 20 mL of the Nisin-CNC nanocomposite solution was sprayed onto the paper surface at a spray angle of 90° within a distance of 20 cm from the kitchen paper using a spray gun, and then vacuum dried and stored at 95°C.

[0056] According to the above method, three parallel experiments were performed.

[0057] Example 2

[0058] The same raw materials and preparation method as in Example 1 were used to adjust the mass concentration of Nisin in the Nisin-CNC nanocomposite solution to 0.02%.

[0059] According to the above method, three parallel experiments were performed.

[0060] Example 3

[0061] The same raw materials and preparation method as in Example 1 were used to adjust the mass concentration of Nisin in the Nisin-CNC nanocomposite solution to 0.03%.

[0062] According to the above method, three parallel experiments were performed.

[0063] Example 4

[0064] The same raw materials and preparation method as in Example 1 were used, the mass concentration of Nisin in the Nisin-CNC nanocomposite solution was adjusted to 0.02%, and the spraying amount was adjusted to 25 mL.

[0065] According to the above method, three parallel experiments were performed.

[0066] Example 5

[0067] Using the same raw materials and preparation method as in Example 1, the mass concentration of Nisin in the Nisin-CNC nanocomposite solution was adjusted to 0.03%, and the spraying amount was adjusted to 25 mL.

[0068] According to the above method, three parallel experiments were performed.

[0069] Comparative Example 1

[0070] The same raw materials and preparation method as in Example 1 were used without adding CNC and Nisin.

[0071] That is, weigh 360g (absolute dry weight) of bleached bamboo pulp board, soak the pulp board with 5L of water for more than 4h, and then tear the obtained pulp board into small pieces of about 4cm×4cm for pulping. Add 18L of deionized water with a temperature of 20°C into the pulp tank of the Wally pulper, start the pulper, and slowly add the pulp into the pulper, control the decomposition time to 5-8min, and decompose until there are no small pulp lumps. Take out the decompressed pulp and dry the water, break it up and put it into a sealed bag, put it in the refrigerator to even out the moisture, measure the moisture content, and refrigerate it for use; the refrigerated pulp is refined by PFI to 30-40°SR, and then decompressed by a decompressor at 7000r until the pulp is completely dispersed into single fibers, and copied into several pieces with a quantitative of 40g / m using a rapid paper sheet former. 2 After the hand sheet is formed, it is vacuum dried and stored at 95°C.

[0072] According to the above method, three parallel experiments were performed.

[0073] Comparative Example 2

[0074] The same raw materials and preparation method as in Example 1 were used, except that the addition of the Nisin-CNC nanocomposite solution was replaced by adding only a Nisin solution with a mass concentration of 0.01%, and no CNC solution was added.

[0075] According to the above method, three parallel experiments were performed.

[0076] Comparative Example 3

[0077] The same raw materials and preparation method as in Example 1 were used, except that only a Nisin solution with a mass concentration of 0.02% was added to the Nisin-CNC nanocomposite solution, and no CNC solution was added.

[0078] According to the above method, three parallel experiments were performed.

[0079] Comparative Example 4

[0080] The same raw materials and preparation method as in Example 1 were used, except that only a Nisin solution with a mass concentration of 0.025% was added to the Nisin-CNC nanocomposite solution, and no CNC solution was added.

[0081] According to the above method, three parallel experiments were performed.

[0082] Comparative Example 5

[0083] The same raw materials and preparation method as in Example 1 were used, except that only a Nisin solution with a mass concentration of 0.03% was added to the Nisin-CNC nanocomposite solution, and no CNC solution was added.

[0084] According to the above method, three parallel experiments were performed.

[0085] Comparative Example 6

[0086] The same preparation method as in Example 1 was adopted, without adding CNC and Nisin, and the raw material was replaced with bleached coniferous kraft pulp. That is, 360g (absolute dry mass) of bleached coniferous kraft pulp board was weighed, and the bleached coniferous kraft pulp board was soaked in 5L of water for more than 4h, and then the obtained pulp board was torn into small pieces of about 4cm×4cm for pulping. 18L of deionized water at a temperature of 20°C was added to the pulp tank of the Wall-E pulping machine. After starting the pulping machine, the pulp was slowly added to the pulping machine, and the decomposition time was controlled at 5-8min, and the pulp was decompressed until there were no small pulp lumps. The decompressed pulp was taken out and dried, and the pulp was broken up and put into a sealed bag. After being put into a refrigerator to evenly distribute the moisture, the moisture content was measured and refrigerated for use; the refrigerated pulp was refined by PFI to 30-40°SR, and then the pulp was decompressed by a decompressor at 7000r until the pulp was completely dispersed into single fibers, and a rapid paper sheet former was used to make several sheets with a quantitative of 40g / m 2 After the hand sheet is formed, it is vacuum dried and stored at 95°C.

[0087] According to the above method, three parallel experiments were performed.

[0088] Performance Testing

[0089] The antibacterial property of the paper-made kitchen paper was tested by the inhibition zone method to detect the antibacterial effect on Gram-positive bacteria. Inhibition zone method: 1. Staphylococcus aureus and Escherichia coli were cultured in a liquid culture medium (LB: peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH=7.8) for 24h to obtain a bacterial culture liquid.

[0090] 2. Prepare plate culture medium: Take an appropriate amount of agar powder and heat it in deionized water to dissolve it, then sterilize it at high temperature. After sterilization, transfer it to the sterilized plate and set it aside after it solidifies naturally.

[0091] 3. Use a 6mm diameter round hole paper cutter to cut the kitchen paper with antibacterial effect into experimental samples for later use.

[0092] 4. Dilute the bacterial culture solution in LB medium to 10 4 ~10 5 CFU / mL of liquid culture medium.

[0093] 5. Use the spread plate method, take 10 μL of the bacterial culture solution and spread it on the plate culture medium, use a sterilized spreader stick to evenly distribute the colonies, and then place the kitchen paper sample on the plate and mark it.

[0094] 6. Place the prepared sample in an incubator for incubation (37°C, 24h), and measure the diameter of the inhibition zone after incubation. The results are shown in Table 1:

[0095] Table 1 Antibacterial properties of broad-spectrum sterilization kitchen paper prepared in Examples 1 to 5 and Comparative Examples 1 to 6

[0096]

[0097] It can be seen from Table 1 that the kitchen paper with broad-spectrum bactericidal performance prepared by the preparation method provided by the present invention has a good antibacterial effect, and the preparation method provided by the present invention has low cost and is suitable for large-scale production.

[0098] It can be seen from Comparative Example 1 that bamboo fiber paper has a certain antibacterial effect on the two experimental bacteria.

[0099] Comparative Examples 2 to 5 show that, compared with Example 1, the antibacterial effect of the paper without CNC but with the same other conditions is not as good as that of the paper with CNC, indicating that the addition of CNC has a certain effect and CNC can improve the retention of Nisin on paper. It also shows that with the increase of Nisin concentration, the antibacterial effect of kitchen paper on the two bacteria becomes better.

[0100] It can be seen from Comparative Example 6 that when the raw material is replaced with bleached softwood kraft pulp and CNC and Nisin are not added, the kitchen paper produced has no antibacterial effect. Compared with the previous example, it shows that bamboo fiber and Nisin have antibacterial effects.

[0101] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for preparing kitchen paper with broad-spectrum bactericidal performance, comprising the following steps: Under acidic conditions, cellulose nanocrystals, nisin and water are mixed to obtain a nanocomposite solution; The nanocomposite solution is sprayed onto the surface of the formed hand-sheet to obtain the kitchen paper with broad-spectrum bactericidal performance.

2. The preparation method according to claim 1, characterized in that: The mixing of cellulose nanocrystals, nisin and water under acidic conditions comprises: mixing cellulose nanocrystals and water, adjusting the pH value of the obtained suspension to be acidic, and then adding nisin.

3. The preparation method according to claim 1 or 2, characterized in that: The pH value of the acidic condition is 4-5.

4. The preparation method according to claim 1 or 2, characterized in that: The mass of the cellulose nanocrystals is 0.06% of the total mass of the cellulose nanocrystals and water.

5. The preparation method according to claim 1 or 2, characterized in that: The mass concentration of nisin in the nanocomposite solution is 0.01-0.03%.

6. The preparation method according to claim 1 or 2, characterized in that: The spraying amount of the nanocomposite solution is 600-700 mL / m 2 Handwritten piece.

7. The preparation method according to claim 1 or 2, characterized in that: The spraying angle is 60-90°, and the spraying distance is 15-20 cm.

8. The preparation method according to claim 1, characterized in that: The basis weight of the hand sheet is 40-50 g / m 2 .

9. The preparation method according to claim 1 or 8, characterized in that: The preparation of the shaped hand sheet comprises the following steps: Soaking the bleached bamboo pulp board in the first water, tearing the obtained pulp board into shredded paper pieces, then adding the second water, performing the first decomposition, drying the water, and then breaking up to obtain pulp; A third water is added to the slurry for beating, and then the slurry is secondarily deflated into single fibers, and then paper-made to obtain a formed hand sheet.

10. Kitchen paper with broad-spectrum bactericidal performance prepared by the preparation method according to any one of claims 1 to 9.

Images