Slide for mammal in-vivo micronucleus test and detection method

Abstract

The invention is suitable for mammal in-vivo micronucleus test detection analysis, and provides a slide for mammal in-vivo micronucleus test and a detection method.The slide is of a double-layer structure and comprises a lower slide body and an upper slide body, and a sample adding groove is formed in the position, under one end of the upper slide body, of the top face of the lower slide body; the upper slide covers the sample adding groove; the detection method of the slide for the in-vivo micronucleus test of the mammal comprises the following steps: S100, inserting a fine needle cylinder into the bone marrow of an experimental body, and extracting the bone marrow; s200, injecting serum into the EP tube, then adding the extracted bone marrow into the EP tube, and uniformly mixing to prepare a mixed solution; s300, adding the mixed solution into a sample adding tank, and enabling the mixed solution to completely and flatly flow to the whole sample adding tank; and S400, after the mixed solution is completely dried, tearing the upper slide, fixing and dyeing, and finally carrying out microscopic examination to complete micronucleus reading. According to the scheme, the bone marrow film preparation effect can be better improved, and the film reading efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of micronucleus test detection and analysis in mammals, in particular to a glass slide for micronucleus test in mammals and a detection method. Background Art

[0002] The mammalian in vivo micronucleus test analyzes the nucleated red blood cells in the animal's bone marrow to detect damage to the nucleated red blood cell chromosomes or mitosis caused by various test substances (including pesticides, chemicals, cosmetics, medical devices, etc.). Therefore, the bone marrow extraction and preparation steps are particularly important.

[0003] like Figure 2 As shown, the current bone marrow extraction process is relatively crude and has the following operational defects:

[0004] 1. After removing the mouse femur, it is easy to crush the fragile femur due to excessive force when squeezing out the bone marrow with hemostatic forceps, resulting in a large amount of bone fragments on the glass slide that are difficult to clean and mixed with impurities, affecting cell observation;

[0005] 2. In the process of smearing the bone marrow mixture to make slices, inconsistent manual operation techniques will lead to uneven slice preparation results, uneven cell dispersion, and easy stacking, resulting in unsatisfactory slice preparation results;

[0006] 3. The amount of bone marrow extracted is not uniform. The successful extraction of bone marrow cells is judged purely by color, which leads to unsatisfactory film production and affects cell counts.

[0007] The above factors are factors that lead to the difficulty in reading micronuclei. In view of this, the present invention transforms the glass slide and proposes a glass slide for micronucleus test in mammals and a detection method. Summary of the invention

[0008] The object of the present invention is to provide a slide for micronucleus test in mammals and a detection method to solve the problems in the above-mentioned background technology.

[0009] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: In a first aspect, the present invention provides a glass slide for micronucleus test in mammals, wherein the glass slide is a double-layer structure, comprising a lower glass slide and an upper glass slide, wherein the top surface of the lower glass slide is located directly below one end of the upper glass slide and is provided with a sample loading groove, wherein the upper glass slide covers the sample loading groove, wherein the upper glass slide is adhered to the lower glass slide, and an easy-tear strip is provided at one end of the upper glass slide away from the sample loading groove, wherein the sample loading groove is located on the outer side of the upper glass slide and is connected to a notch, wherein the notch is semicircular.

[0010] Preferably, the lower glass slide is 55 mm long and 25 mm wide.

[0011] Preferably, the upper glass slide is a glass film, and the upper glass slide is 45 mm long and 19 mm wide.

[0012] Preferably, the depth of the sample adding groove is 0.1 mm.

[0013] The second aspect of the present invention provides a method for detecting a micronucleus test in a mammal using the glass slide described in the first aspect of the present invention, comprising the following steps:

[0014] S100, using a tiny needle, inserted into the subject's bone marrow to extract bone marrow;

[0015] S200, injecting serum into the EP tube, then adding the extracted bone marrow into the EP tube, mixing well, and preparing a mixed solution;

[0016] S300 adds the mixed solution into the sample adding slot through the notch, and allows the mixed solution to flow completely to the entire sample adding slot;

[0017] S400, after the mixed solution is completely dry, the upper glass slide is torn off, fixed and stained, and finally microscopic examination is performed to complete the micronucleus reading.

[0018] Preferably, the volume of the mixed solution is 0.1 mL.

[0019] The present invention has at least the following beneficial effects:

[0020] The present invention provides a glass slide and a detection method for a micronucleus test in a mammal. The bone marrow is extracted by a syringe or the like, then added to an EP tube and mixed with serum, the prepared mixed solution is poured into a sample adding groove, and after the mixed solution is completely advected to the entire sample adding groove and completely dried, the upper glass slide is torn off and subsequent operations are continued, thereby better improving the effect of bone marrow slide preparation and improving the efficiency of film reading. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the glass slide structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the micronucleus test inspection and analysis process in mammals under conventional operations in the prior art;

[0023] Figure 3 This is a schematic diagram of the improved micronucleus test in mammals in vivo analysis process of the present invention;

[0024] Figure 4 This is a comparison chart of the conventional method and the lens observation of the present invention.

[0025] In the accompanying drawings: 1, lower glass slide; 2, upper glass slide; 3, easy-tear strip; 4, sample loading slot. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example

[0028] like Figure 1 As shown, the present invention provides a glass slide for micronucleus test in mammals, the glass slide is a double-layer structure, the glass slide comprises a lower glass slide 1 and an upper glass slide 2, the top surface of the lower glass slide 1 is located just below one end of the upper glass slide 2 and is provided with a sample loading groove 4, the upper glass slide 2 covers the sample loading groove 4, the upper glass slide 2 is adhered to the lower glass slide 1, and an easy-tear strip 3 is provided at one end of the upper glass slide 2 away from the sample loading groove 4, specifically, the easy-tear strip 3 is fixedly connected to the lower glass slide 1; the lower glass slide 1 is 55 mm long and 25 mm wide; the upper glass slide 2 is a glass film, the upper glass slide 2 is 45 mm long and 19 mm wide; the depth of the sample loading groove 4 is 0.1 mm, the sample loading groove 4 is located on the outer side of the upper glass slide 2 and is connected to a notch, and the notch is semicircular.

[0029] The present invention improves the glass slide and then applies it to the micronucleus test in mammals to detect and analyze the micronucleus, which can better improve the effect of bone marrow slide preparation and improve the efficiency of slide reading. Figure 3 As shown, the glass slide prepared by the present invention is used for the detection method of micronucleus test in mammals, and the specific steps are as follows:

[0030] S100, in view of the fact that improper operation of hemostatic forceps can easily lead to the introduction of impurities, a fine syringe or a tool similar to an egg retrieval needle is used to insert it into the bone marrow of the experimental subject to extract the bone marrow;

[0031] S200, inject serum into the EP tube, then add the extracted bone marrow into the EP tube, mix well, and prepare a mixed solution, the mixed solution is 0.1mL;

[0032] S300, adding 0.1 mL of the mixed solution into the sample loading slot 4 through the notch, and allowing the mixed solution to completely flow to the entire sample loading slot 4, thereby preventing cell accumulation;

[0033] S400, after the mixed solution is completely dried, the upper glass slide 2 is torn off, fixed and stained, and finally microscopically examined to complete the micronucleus reading.

[0034] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A slide for micronucleus test in mammals, characterized in that: The glass slide is a double-layer structure, comprising a lower glass slide (1) and an upper glass slide (2); the top surface of the lower glass slide (1) is provided with a sample loading groove (4) located just below one end of the upper glass slide (2); the upper glass slide (2) covers the sample loading groove (4); the upper glass slide (2) is adhered to the lower glass slide (1); an easy-tear strip (3) is provided at one end of the upper glass slide (2) away from the sample loading groove (4); the sample loading groove (4) is located on the outer side of the upper glass slide (2) and is connected to a notch, and the notch is semicircular.

2. A slide for micronucleus test in mammals according to claim 1, characterized in that: The lower glass slide (1) is 55 mm long and 25 mm wide.

3. A slide for micronucleus test in mammals according to claim 1, characterized in that: The upper glass slide (2) is a glass film, and the upper glass slide (2) is 45 mm long and 19 mm wide.

4. A glass slide for micronucleus test in mammals according to claim 1, characterized in that: The depth of the sample adding groove (4) is 0.1 mm.

5. The method for detecting micronucleus test in mammals using the glass slide as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S100, using a tiny needle, inserted into the subject's bone marrow to extract bone marrow; S200, injecting serum into the EP tube, then adding the extracted bone marrow into the EP tube, mixing well, and preparing a mixed solution; S300, adding the mixed solution into the sample adding groove (4) through the notch, and allowing the mixed solution to completely flow to the entire sample adding groove (4); S400, after the mixed solution is completely dried, the upper glass slide (2) is torn off, fixed and stained, and finally microscopically examined to complete the micronucleus reading.

6. The detection method according to claim 5, characterized in that: The volume of the mixed solution is 0.1 mL.

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