Intelligent turbidimetric apparatus

Abstract

The invention discloses an intelligent turbidimetric instrument, belongs to the field of medical instruments, and particularly relates to a bacterium liquid preparation process of a drug sensitivity experiment. The method is mainly used for bacterial turbidity detection, drug sensitivity experiments and other application scenes in the field of microbiological detection. According to the present invention, the phenomena of repeated sampling liquid preparation and concentration determination by using a turbidimetric instrument exist in the manual preparation of the bacterial liquid, the step is tedious in the drug sensitivity experiment, and in order to reduce the manual operation of the step, two specific technical implementation schemes are introduced, and the instrument selection can be performed according to the specific conditions; according to the technical scheme, the intelligent turbidimeter is used for detecting the concentration of a sample, whether a sample bacterial solution meets the required concentration in a drug sensitivity experiment or not is automatically judged, if not, the volume of a required normal saline diluent is calculated by extracting a micro control unit through a peristaltic pump, and the volume of the normal saline diluent is added into a sample test tube; after the sample is diluted, starting the vibration motor to uniformly shake the sample, and re-measuring the concentration by the turbidimetric instrument to obtain the bacterial liquid for the subsequent drug sensitivity experiment. According to the other technical scheme, on the basis of automatically judging the concentration of the bacterial liquid, the bacterial liquid for the drug sensitivity experiment is prepared in a high-flux liquid preparation mode.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, and in particular to an intelligent turbidimeter. Background Art

[0002] Optical principle: McFarland turbidimetry is a method used for turbidity measurement. It uses the principle of light scattering by solid particles to measure the turbidity of the liquid. When visible light passes through a liquid, the solid particles in it will scatter the light. The intensity of the scattered light is proportional to the number and size of the particles, that is, proportional to the turbidity of the liquid. According to the Lorentz-Boltzmann formula, the turbidity value of the liquid is related to the concentration, size and refractive index of the particles in the liquid. Using the characteristic that scattered light is proportional to the concentration and size of the particles, the turbidity can be measured by comparing the difference between the liquid to be tested and the known standard turbid liquid, and then the particle concentration in the liquid can be obtained. In drug sensitivity experiments, the concentration of the bacterial liquid can be determined based on the turbidity.

[0003] Calibration and establishment of standard curve: In the McFarland turbidimetry, a suspension formed by a quantitative amount of barium chloride solution and sulfuric acid solution is used as the standard solution for turbidity measurement. A standard curve is established based on the correspondence between the turbidity of the barium sulfate precipitate in the solution and the approximate concentration of the actual bacterial culture.

[0004] At present, the turbidimeters used for drug sensitivity tests on the market do not have the function of automatically configuring bacterial solutions, and require the experimenter to repeatedly select bacteria, add samples, and read the turbidimeter values, which is cumbersome and inefficient. Therefore, the present invention automatically reads and adds samples to the samples, and finally achieves the effect of automated intelligent turbidimetry, which solves the inconvenience of operation encountered by the experimenter in the drug sensitivity test and improves the overall efficiency of the drug sensitivity test. Summary of the invention

[0005] The purpose of the present invention is to provide an intelligent turbidimeter, and propose two model schemes for pipetting sampling and vibration shaking mode, aiming to solve the problem that the turbidimeter repeatedly performs turbidimetric liquid preparation operations of the same sample in drug sensitivity experiments.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] Bacterial liquid concentration determination

[0008] The light source is used to illuminate a standard solution of a certain concentration and volume of barium chloride and sulfuric acid solution, and the size of the actual signal is detected by a photoelectric detector. By setting the electrical signal intensity as a standard in the program, a linear relationship is established with the bacterial solution concentration. Different actual bacterial solution samples are used under the same light source irradiation conditions to obtain different electrical signal intensities, thereby achieving the determination of the bacterial solution concentration.

[0009] Instrument construction

[0010] The turbidimeter is divided into a detection part, an intelligent sample addition part, a display and interaction part, an instrument housing and a stabilization part, and a disinfection and sterilization part. The detection part is mainly composed of a light source (6), a photoelectric receiver (7) and a micro control unit (7), wherein the light source is powered by a stable constant current source to ensure the consistency of the luminous intensity during detection; the photoelectric receiver (7) is composed of a photodiode, a signal amplification circuit and an A / D conversion circuit, and can convert the light intensity signal into a measurable voltage signal; the micro control unit (7) measures the sampled voltage signal and calculates the current bacterial solution concentration using a software program. The intelligent sample addition part is mainly composed of a sample addition module (1, 2) and a vibration module (9), wherein the intelligent sample addition module uses a sample addition needle as a sample addition tool to ensure that the liquid flow rate remains stable, and the peristaltic pump is responsible for extracting an appropriate amount of physiological saline to dilute the current bacterial solution. After the micro control unit reads the current concentration, it calculates the volume of physiological saline required for the bacterial solution to obtain the ideal bacterial solution concentration in the drug sensitivity test. After the control signal is sent by the micro control unit, the peristaltic pump extracts an equal amount of physiological saline and adds it to the sample. The vibration module vibrates after the physiological saline is added, and the sample is shaken using a vibration motor or ultrasonic vibration generator according to actual needs; the display and interaction part uses the Android display as a display window for the sample concentration, and the required numerical value can be entered to dilute the sample; the instrument housing and the stable part are mainly composed of a square housing and four stable feet to ensure the stability of the instrument; the disinfection and sterilization part uses an ultraviolet disinfection lamp, which can stabilize and sterilize the instrument after each experiment.

[0011] Beneficial Effects

[0012] Compared with the turbidimeters on the market, the advantages of the present invention are that the entire instrument is easy to use, there are many options to choose from, and the vibration module can be selected according to the specific situation. A design scheme of a high-throughput intelligent turbidimeter is given, which solves the problem of the experimenter manually preparing the bacterial solution for repeated sampling and using the turbidimeter for concentration measurement when doing drug sensitivity tests. It not only improves the efficiency of drug sensitivity tests, but also reduces the workload of experimenters. At the same time, it also reduces the contact between experimenters and pathogens to a certain extent, improves the accuracy of liquid preparation, and protects the health and safety of experimenters.

[0013] This application document provides an overview of various implementations or examples of the technology described in this disclosure, which is illustrative and not a comprehensive disclosure of all features. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 , Figure 2 A schematic diagram of the structure of two specific embodiments of an intelligent turbidimeter provided by the present invention;

[0015] Figure 1In: 1. Peristaltic pump; 2. Sample needle; 3. Android display screen; 4. Instrument cover and UV disinfection lamp; 5. Sample test tube; 6. Light source; 7. Photoelectric receiver and micro control unit; 8. Instrument housing; 9. Vibration motor; 10. Instrument foot pad; 11 UV disinfection lamp;

[0016] Figure 2 Middle: 1. Sample test tube; 2. Test tube turntable; 3. Photoelectric receiver; 4. Light source; 5. Stepper motor;

[0017] Figure 3 It is an operation flow chart of an intelligent turbidimeter;

[0018] Figure 4 This is a top view of the test tube turntable, which has 20 sample through holes. Example

[0019] See also Figures 1 to 3 The turbidimeter provided by the present invention mainly includes five parts, namely, a detection part (5, 6, 7), an intelligent sample addition part (1, 2, 9), a display and interaction part (3, 4), an instrument housing and a stabilization part (8, 10) and a disinfection and sterilization part (11).

[0020] The detection part is mainly composed of a light source (6), a photoelectric receiver (7) and a micro control unit (7). The light source is powered by a stable constant current source to ensure the consistency of the luminous intensity during detection. The photoelectric receiver is composed of a photodiode, a signal amplification circuit and an A / D conversion circuit, and can convert the light intensity signal into a measurable voltage signal. The micro control unit (7) measures the sampled voltage signal and calculates the current bacterial liquid concentration using a software program. Compared with the single-channel detection, the high-throughput detection part adds a stepper motor and a test tube turntable, and the turntable is precisely controlled by the motor to rotate to the photoelectric detection area to achieve high-throughput detection.

[0021] The intelligent sample addition part can be selected according to actual needs. Figure 1 The way: Figure 1 The device is mainly composed of a peristaltic pump (1), a sample addition needle (2) and a vibration motor (9). The sample addition needle is used as a sample addition tool to ensure the stability of the liquid flow rate. The peristaltic pump is responsible for extracting an appropriate amount of physiological saline to dilute the current bacterial solution, and after the micro-control unit reads the current concentration, the volume of physiological saline required for the bacterial solution is calculated to obtain the ideal bacterial solution concentration in the drug sensitivity test. After the control signal is sent by the micro-control unit, the peristaltic pump extracts an equal amount of physiological saline and adds it to the sample to achieve accurate sample addition.

[0022] After the sample is added, the vibration module shakes the sample to ensure uniform mixing of the sample.

[0023] The display and interaction part uses the Android display as a display window for sample concentration, and the required value can be entered to dilute the sample. The user can operate the device by touching the screen, and can understand the current working status of the device through the prompt information on the interface.

[0024] After the experiment is completed, the disinfection and sterilization part of the instrument will automatically turn on for a few minutes to disinfect and sterilize the instrument to ensure the safety and sterility of the instrument.

[0025] The present invention mainly uses the operation process as follows Figure 3 Before use, the user prepares the sample solution to be prepared, turns on the smart turbidimeter, and performs self-calibration. Place the solution sample tube into the test tube slot, close the instrument cover, enter the required bacterial solution concentration on the Android display, and initialize the program to perform the detection operation. The instrument self-judges the bacterial solution concentration, and obtains the required bacterial solution through intelligent sample addition partial dilution and oscillation. Wait for the operator to take out the sample solution to determine whether to perform the turbidity measurement and solution preparation of the next sample. When all sample solutions are prepared, turn on the ultraviolet disinfection lamp and shut down after running for a few minutes.

[0026] Therefore, the present invention provides a turbidimeter for detecting bacterial liquid concentration, which has the advantages of high detection accuracy, high degree of automation and easy operation, and can be applied to hospital clinical drug sensitivity laboratories, scientific research institutions and other fields.

[0027] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim involved.

[0028] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. An intelligent turbidimeter, characterized in that: The invention comprises a peristaltic pump (1), a sample adding needle (2), an Android display screen (3), an instrument cover (4), a sample test tube (5), a light source (6), a photoelectric receiver (7) and a micro control unit (7), an instrument housing (8), a vibration module (9), an instrument foot pad (10), an ultraviolet disinfection lamp (11), etc. The invention is characterized in that the light source is an LED light source, which is closely attached to the sample test tube slot. The photoelectric receiver is on the opposite side of the sample test tube slot, the sensor is a photodiode, and a micro control unit is provided on the PCB board. A vibration module is installed on the bottom side of the sample test tube slot, and a vibration motor or an ultrasonic vibration generator is available for selection, which can vibrate and shake the sample test tube when started. A sampling module is provided directly above the sample test tube slot. Two schemes are designed for the sampling module. One is shown in Figure 1. One end of the hose is connected to the sample needle fixed on the instrument cover, and the other end is connected to the peristaltic pump. The peristaltic pump can control the amount of sample from the solution bottle to the needle to dilute the sample. The high-throughput intelligent turbidimeter is shown in Figure 2. The test tube turntable has 20 through holes, which can fix the test tubes. The light source and the photoelectric receiver are fixed at both ends of a through hole. The stepper motor can control the rotation of the test tube turntable. The Android display is fixed on the instrument cover.

2. The intelligent turbidimeter according to claim 1, characterized in that: The light source (6) is an LED light source with selectable specific wavelengths of 550 nm, 560 nm, 570 nm, 580 nm, 590 nm, 600 nm, 610 nm, 620 nm, 630 nm, 640 nm, and 650 nm, and is used for turbidity measurement.

3. The intelligent turbidimeter according to claim 1, characterized in that: The instrument cover (4) is provided with an Android display screen (3) and an ultraviolet disinfection lamp (11), and one side of the cover is fixed to the instrument as a whole via a hinge.

4. The intelligent turbidimeter according to claim 1, characterized in that: A vibration module (9) is provided at the bottom of the sample test tube slot. The module can be a vibration motor or an ultrasonic vibration generator and can be replaced as needed.

5. The intelligent turbidimeter according to claim 1, characterized in that: The sampling modules (1, 2) are peristaltic pumps and sampling needles for controlling flow rate and sampling.

6. The intelligent turbidimeter according to claim 1, characterized in that: The micro control unit (7) has the functions of calculating the required diluent volume, controlling the vibration of the vibration module, controlling the sampling of the peristaltic pump, controlling the displacement of the stepping motor, controlling the display of the Android display screen, and reading the data of the photoelectric receiver.

7. The intelligent turbidimeter according to claim 1, characterized in that: The ultraviolet disinfection lamp (11) will automatically turn on after the sample bacterial solution preparation operation is completed, thereby ensuring a sterile environment after repeated testing.

8. The intelligent turbidimeter according to claim 1, characterized in that: The intelligent turbidimeter has the function of high-throughput sample detection. The high-throughput scheme is specifically shown in FIG2 , and the test tube turntable is controlled by a stepper motor to rotate to the photoelectric detection area.

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