Efficient sewage treatment biological membrane technology for sewage station

By using a variety of microbial biofilms with optimized proportions and integrated reverse osmosis devices in sewage treatment, the problems of low efficiency and insufficient stability of existing sewage treatment methods are solved, and efficient and stable sewage treatment effects are achieved, meeting the increasingly strict conditions. environmental protection requirements.

CN119219211APending Publication Date: 2024-12-31韩俊喜
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Patent Information

Application Number
CN202411487975.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-24
Publication Date
2024-12-31

AI Technical Summary

Technical Problem

The existing sewage treatment methods are inefficient, the treatment effect is not up to standard, the microbial community is not optimized enough, the biofilm preparation process is incomplete, and the performance and automation control level of reverse osmosis device are insufficient, resulting in unstable treatment effect.

Method used

Using biofilms containing nitrified bacteria, denitrified bacteria and Bacillus, with a ratio of 3:2:1, and adding activated carbon and zeolite powder, equipped with an integrated reverse osmosis device, including a vertical multi-stage stainless steel pump and automatic control system, to ensure Microbial activity and treatment efficiency.

Benefits of technology

It achieves a more efficient and stable sewage treatment effect, can comprehensively remove pollutants in sewage, improves the water quality to meet environmental protection requirements, and enhances the adsorption capacity of biofilms and the performance stability of the treatment system.

✦ Generated by Eureka AI based on patent content.
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Abstract

The invention provides an efficient sewage treatment biological membrane technology for a sewage station, and belongs to the technical field of sewage treatment.The efficient sewage treatment biological membrane technology is characterized in that nitrifying bacteria, denitrifying bacteria and bacillus are optimally combined according to the proportion of 3: 2: 1 on the aspect of microorganism composition, various pollutants in sewage can be comprehensively and efficiently removed, the nitrogen content is reduced, organic matter is decomposed, and the sewage treatment efficiency is improved. Activated carbon and zeolite powder are added to enhance the adsorption capacity of the biological membrane and remove impurities and harmful substances, the well-designed preparation technology guarantees the efficient performance of the biological membrane, specific culture conditions and strict control of all links ensure the stable structure and uniform performance of the biological membrane, the reverse osmosis device improves the performance of the system, the structure is compact, the treatment capacity is high, and operation is easy and convenient. The RO high-pressure pump is excellent in performance and guarantees system stability, key factors are controlled through a scientific biological membrane using method, a suitable environment is provided for microorganisms, the treatment effect is improved, in a word, traditional limitation is overcome, efficient, stable and environment-friendly sewage treatment is achieved, and remarkable contribution is made to environment protection and sustainable development.
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Description

Technical Field

[0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a biofilm technology for efficient sewage treatment in a sewage station. Background Art

[0002] In today's society, sewage treatment has become an important issue for environmental protection and sustainable development. Traditional sewage treatment methods often have problems such as low efficiency and substandard treatment effects, and it is difficult to meet increasingly stringent environmental protection requirements. If organic matter, nitrogen, phosphorus and other pollutants in sewage cannot be effectively removed, it will pose a serious threat to the ecological environment and human health. Therefore, it is urgent to develop efficient sewage treatment technologies and methods. In this context, biological treatment technology has attracted much attention due to its advantages of high efficiency, environmental protection and low cost. However, existing biological treatment methods still have some limitations, such as the composition of microbial communities is not optimized enough, resulting in unstable treatment effects; the conditions in the treatment process are not accurately controlled, affecting the activity and treatment efficiency of microorganisms. For example, the biofilms used in some sewage stations may only contain a single or a few microorganisms, which cannot fully and effectively remove various pollutants in sewage. Moreover, the preparation process of the biofilm may not be perfect enough to give full play to the role of microorganisms. In terms of the treatment system, the performance and automation control level of the reverse osmosis device also need to be improved to ensure the stability and reliability of the treatment effect.

[0003] In order to overcome these problems, the present invention proposes a high-efficiency sewage treatment biofilm for a sewage station containing multiple microorganisms with optimized proportions, and its preparation technology and use method, and is equipped with an advanced integrated reverse osmosis device, aiming to achieve more efficient and stable sewage treatment effects. Summary of the invention

[0004] The purpose of the present invention is to provide a high-efficiency sewage treatment biofilm technology for a sewage station, aiming to solve the problems in the prior art.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A highly efficient sewage treatment biofilm for a sewage station, characterized in that the biofilm comprises nitrifying bacteria, denitrifying bacteria and Bacillus, and the ratio of each microorganism is 3:2:1.

[0007] As a preferred solution of the present invention, the biofilm further comprises activated carbon and zeolite powder, with contents of 15% and 10% respectively.

[0008] As a preferred solution of the present invention, a technology for preparing a biofilm for efficient sewage treatment in a sewage station is characterized by comprising the following steps:

[0009] Step 1: Cultivate nitrifying bacteria, denitrifying bacteria and Bacillus in beef extract peptone medium, LB medium and nutrient broth medium respectively, the culture conditions are temperature 30°C, pH value 7.0, culture time is 48 hours, and ammonium chloride is added during the culture of nitrifying bacteria, the addition amount is 5g / L;

[0010] Step 2: Mix the cultured microorganisms in a ratio of 3:2:1;

[0011] Step 3: Add activated carbon and zeolite powder to the mixed microorganisms and stir evenly at 200 r / min;

[0012] Step 4: The uniformly stirred mixture is formed into a biofilm under molding conditions of 5 MPa and 40° C., and the molding time is maintained for 2 hours.

[0013] As a preferred solution of the present invention, a reverse osmosis system using an integrated reverse osmosis device is included, the equipment structure is compact and reasonable, and the single set processing capacity is ≧25m 3 / h, single RO membrane water output ≧1.0t / h, single RO membrane desalination rate >99.5%, reverse osmosis membrane uses dry membrane. Control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated on the panel for easy operation.

[0014] As a preferred solution of the present invention, the RO high-pressure pump adopts a vertical multi-stage stainless steel pump. The start and stop of the water pump is interlocked with the liquid level signal of each process water tank. Manual valves are installed at the pump inlet and outlet, and a check valve and pressure gauge are installed at the outlet. Manual operation is also possible on site. Water pump flow ≧36m 3 / h, head ≧140m, material 304 stainless steel, protection grade not less than IP55.

[0015] As a preferred solution of the present invention, the startup, operation, flushing and other processes of the reverse osmosis system can be automatically controlled. The design flux of the RO membrane element is not greater than the maximum flux value specified in the "Guidelines" of the membrane element manufacturer, and a reasonable arrangement and combination is selected to ensure the normal operation of the membrane element and a reasonable cleaning cycle.

[0016] As a preferred solution of the present invention, the biofilm is placed in a sewage treatment pool, the sewage stays in the pool for no less than 8 hours, the contact area between the biofilm and the sewage is no less than 30 square meters, and the dissolved oxygen concentration in the pool is maintained at 2-5 mg / L during the treatment process.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] In terms of microbial composition, by rationally matching nitrifying bacteria, denitrifying bacteria and Bacillus, and optimizing the combination in a ratio of 3:2:1, various pollutants in sewage can be removed comprehensively and efficiently. Nitrifying bacteria can convert ammonia nitrogen into nitrite and nitrate, and denitrifying bacteria can further reduce nitrate to nitrogen gas, effectively reducing the nitrogen content in sewage. Bacillus helps to decompose organic matter. The synergistic effect of the three greatly improves the efficiency and effect of sewage treatment, so that the treated water quality can better meet environmental protection requirements. Secondly, the added activated carbon and zeolite powder can enhance the adsorption capacity of the biofilm and further remove impurities and harmful substances in sewage. Activated carbon has excellent adsorption performance and can adsorb organic pollutants and heavy metal ions; zeolite powder can effectively adsorb substances such as ammonia nitrogen. The addition of the two significantly improves the comprehensive treatment capacity of the biofilm. Furthermore, the carefully designed preparation technology provides a guarantee for the high-efficiency performance of the biofilm. Microorganisms are cultured under specific culture conditions to maintain good activity and growth state, and the mixing ratio and stirring conditions of the microorganisms are strictly controlled. The components and molding conditions ensure that the structure of the biofilm is stable and the performance is uniform, so that it can play a stable role in practical applications. The integrated reverse osmosis device has improved the performance of the entire sewage treatment system. It has a compact and reasonable structure, a strong single-set treatment capacity, a single RO membrane with high water production and excellent desalination rate. The control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated in the panel, which is easy to operate and improves work efficiency. The performance of the RO high-pressure pump is excellent, and the start and stop of the water pump is interlocked with the liquid level signal of each process water tank to ensure the stability and reliability of the system operation. Finally, the scientific and reasonable method of using the biofilm provides a suitable living and working environment for microorganisms by controlling the residence time of sewage in the pool, the contact area between the biofilm and sewage, and the dissolved oxygen concentration in the pool, giving full play to the metabolic function of microorganisms and further improving the sewage treatment effect. In summary, the present invention effectively overcomes many limitations of traditional sewage treatment methods, achieves more efficient, more stable and more environmentally friendly sewage treatment effects, and makes important contributions to protecting the environment and promoting sustainable development. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1

[0021] The present invention provides the following technical solutions:

[0022] A highly efficient sewage treatment biofilm for a sewage station, characterized in that the biofilm comprises nitrifying bacteria, denitrifying bacteria and Bacillus, and the ratio of each microorganism is 3:2:1.

[0023] In a specific embodiment of the present invention, nitrifying bacteria can efficiently convert ammonia nitrogen into nitrite and nitrate, and denitrifying bacteria can further reduce nitrate to nitrogen gas. The two work synergistically to greatly reduce the nitrogen content in sewage. Bacillus plays an important role in decomposing organic matter and can effectively remove organic pollutants in sewage. The three microorganisms are combined in a specific proportion to form a microbial community that cooperates with each other and complements each other. For example, when treating industrial sewage containing high concentrations of ammonia nitrogen and complex organic matter, nitrifying bacteria and denitrifying bacteria can quickly reduce the nitrogen content, and Bacillus can simultaneously decompose organic matter, so that the treated water quality can meet strict environmental protection standards in terms of nitrogen content and organic matter indicators, providing a solid foundation for efficient and stable sewage treatment.

[0024] Specifically, the biofilm further comprises activated carbon and zeolite powder, with contents of 15% and 10% respectively.

[0025] In this embodiment: activated carbon has extremely strong adsorption properties and can effectively adsorb harmful substances such as organic pollutants and heavy metal ions, while zeolite powder has excellent adsorption capacity for substances such as ammonia nitrogen. In the actual sewage treatment process, for example, sewage polluted by agricultural non-point sources may contain a large amount of pesticide residues and nitrogen. Activated carbon can adsorb residual pesticide molecules to reduce their harm to the environment, while zeolite powder specifically adsorbs ammonia nitrogen and reduces the content of nitrogen. The addition of the two significantly enhances the adsorption capacity of the biofilm, and synergizes with microorganisms to further remove impurities and harmful substances in sewage, greatly improving the comprehensive effect of sewage treatment, making the treated water quality purer and meeting higher environmental protection requirements.

[0026] Specifically, a preparation technology of a high-efficiency sewage treatment biofilm in a sewage station is characterized by comprising the following steps:

[0027] Step 1: Cultivate nitrifying bacteria, denitrifying bacteria and Bacillus in beef extract peptone medium, LB medium and nutrient broth medium respectively, the culture conditions are temperature 30°C, pH value 7.0, culture time is 48 hours, and ammonium chloride is added during the culture of nitrifying bacteria, the addition amount is 5g / L;

[0028] Step 2: Mix the cultured microorganisms in a ratio of 3:2:1;

[0029] Step 3: Add activated carbon and zeolite powder to the mixed microorganisms and stir evenly at 200 r / min;

[0030] Step 4: The uniformly stirred mixture is formed into a biofilm under molding conditions of 5 MPa and 40° C., and the molding time is maintained for 2 hours.

[0031] In this embodiment: nitrifying bacteria, denitrifying bacteria and Bacillus are cultured in specific culture medium respectively, and the culture conditions are strictly controlled, such as temperature of 30°C, pH value of 7.0 and culture time of 48 hours, which can ensure the full growth and reproduction of microorganisms and maintain good activity and metabolic capacity. Taking nitrifying bacteria as an example, under the specific culture conditions of adding ammonium chloride, its growth rate and ammonia nitrogen conversion efficiency are significantly improved. Then, the microorganisms are mixed in a ratio of 3:2:1. This precise ratio control ensures that different microorganisms play the best synergistic role in subsequent treatment. Then, at 200r / m In the stirring conditions of 5000 g, activated carbon and zeolite powder were added and stirred evenly, which made the components fully mixed and laid the foundation for the formation of biofilm with uniform performance. Finally, the biofilm was made under the molding conditions of 5MPa and 40°C and maintained for 2 hours. The strict molding conditions ensured that the biofilm had a stable structure and good physical properties. When treating urban domestic sewage, the biofilm produced by this preparation technology can quickly adapt to the complex sewage environment and efficiently remove various pollutants, such as organic matter, nitrogen and phosphorus, thereby ensuring that the treated water quality meets the discharge standards and providing strong support for the improvement of urban water environment.

[0032] Specifically, the reverse osmosis system adopts an integrated reverse osmosis device, the equipment structure is compact and reasonable, and the single set processing capacity is ≧25m 3 / h, single RO membrane water output ≧1.0t / h, single RO membrane desalination rate >99.5%, reverse osmosis membrane uses dry membrane. Control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated on the panel for easy operation.

[0033] In this embodiment: the reverse osmosis system equipment has a compact and reasonable structure, saves space, is easy to install and maintain, and has a single set processing capacity of not less than 25m 3 / h, the water output of a single RO membrane is not less than 1.0t / h. The powerful processing capacity can meet the needs of large-scale sewage treatment. The desalination rate of a single RO membrane is greater than 99.5%, which can effectively remove salt and other soluble impurities in sewage and significantly improve water quality. Taking the treatment of groundwater in coastal areas affected by seawater intrusion as an example, the integrated reverse osmosis device can efficiently remove salt from seawater and restore groundwater to usable freshwater resources. The control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated in the panel, which is easy to operate and greatly improves work efficiency. The staff can intuitively monitor and control various parameters in the treatment process, adjust the operation strategy in time, ensure the stable operation of the system, and ensure that the treated water quality always meets the requirements.

[0034] Specifically, the RO high-pressure pump adopts a vertical multi-stage stainless steel pump. The start and stop of the water pump is interlocked with the liquid level signal of each process water tank. Manual valves are installed at the pump inlet and outlet, and a check valve and pressure gauge are installed at the outlet. Manual operation is also possible on site. Water pump flow ≧36m 3 / h, head ≧140m, material 304 stainless steel, protection grade not less than IP55.

[0035] In this embodiment: the flow rate of the water pump is not less than 36m 3 / h, head is not less than 140m, powerful power can ensure the normal operation of the reverse osmosis system, even when dealing with high-concentration sewage, it can maintain a stable working state. For example, when treating industrial wastewater, facing the complex pollutants and high water pressure requirements, this high-performance water pump can smoothly press the sewage into the reverse osmosis membrane for treatment. It is made of 304 stainless steel, with good corrosion resistance and wear resistance, which extends the service life of the water pump and reduces maintenance costs. The protection level is not less than IP55, which can effectively prevent dust and moisture from entering the pump body, ensuring that the water pump can still operate reliably in harsh environments. The start and stop of the water pump is interlocked with the liquid level signals of each process water tank. Manual valves are installed at the inlet and outlet of the pump, and check valves and pressure gauges are installed at the outlet. Manual operation is also possible on site. This design realizes the flexible combination of automatic control and manual operation, which not only improves the operating efficiency of the system, but also ensures that manual intervention can be carried out in an emergency to ensure the safe and stable operation of the system.

[0036] Specifically, the startup, operation, flushing and other processes of the reverse osmosis system can be automatically controlled. The design flux of the RO membrane element is not greater than the maximum flux value specified in the "Guidelines" of the membrane element manufacturer, and a reasonable arrangement and combination is selected to ensure the normal operation of the membrane element and a reasonable cleaning cycle.

[0037] In this embodiment: the automatic control function can significantly improve the operating efficiency and stability of the system. For example, when treating large-scale sewage in an urban sewage treatment plant, the system can automatically adjust the operating parameters according to the real-time changes in water quality and water volume, without frequent manual intervention, which greatly reduces the workload of operators. The design flux of the RO membrane element is not greater than the maximum flux value specified in the "Guidelines" of the membrane element manufacturer, and a reasonable arrangement and combination is selected, which effectively extends the service life of the membrane element and reduces the replacement cost. At the same time, the reasonable arrangement and combination ensures that the membrane element can be evenly stressed during operation, reduces local wear, and improves the reliability of the entire system. Through scientific control and optimized design, the normal operation of the membrane element and a reasonable cleaning cycle can be ensured, thereby maintaining the system's long-term stable and efficient processing capacity, providing a strong guarantee for the continuous and stable provision of high-quality treated water quality.

[0038] Specifically, the biofilm is placed in a sewage treatment pool, the sewage stays in the pool for no less than 8 hours, the contact area between the biofilm and the sewage is no less than 30 square meters, and the dissolved oxygen concentration in the pool is maintained at 2-5 mg / L during the treatment process.

[0039] In this embodiment: the biofilm is placed in a sewage treatment pool, and the sewage is required to stay in the pool for no less than 8 hours, which allows the microorganisms to have sufficient time to fully contact and react with the pollutants in the sewage, thereby achieving a comprehensive and in-depth treatment effect. Taking the treatment of industrial wastewater containing a variety of persistent organic pollutants as an example, a longer residence time ensures that the microorganisms can gradually decompose these difficult-to-degrade substances, improve the thoroughness of the treatment, and ensure that the contact area between the biofilm and the sewage is not less than 30 square meters, providing sufficient space for the action of microorganisms, increasing the contact opportunities between microorganisms and pollutants, and improving the treatment efficiency. During the treatment process, the dissolved oxygen concentration in the pool is maintained at 2-5 mg / L, creating a suitable living and metabolic environment for microorganisms, promoting the growth and reproduction of microorganisms, and enhancing their ability to decompose pollutants. For example, when treating domestic sewage rich in nitrogen and phosphorus, a suitable dissolved oxygen concentration can stimulate the metabolic activity of microorganisms, accelerate the removal process of nitrogen and phosphorus, further improve the sewage treatment effect, and ensure that the treated water quality meets higher environmental protection standards.

[0040] The working principle and use process of the present invention:

[0041] First, microorganisms need to be cultured. Nitrifying bacteria, denitrifying bacteria and Bacillus are cultured in specific beef extract peptone medium, LB medium and nutrient broth medium, respectively. The culture conditions are strictly controlled at a temperature of 30°C and a pH value of 7.0. The culture time is 48 hours. In the culture process of nitrifying bacteria, 5g / L of ammonium chloride needs to be added to meet its growth needs. After the culture is completed, the three microorganisms are mixed in a ratio of 3:2:1. Subsequently, activated carbon and zeolite powder with contents of 15% and 10% respectively are added to the mixed microorganisms, and stirred evenly at 200r / min. Then, the evenly stirred mixture is made into a biofilm under the molding conditions of 5MPa and 40°C, and the molding time is maintained for 2 hours. In the sewage treatment system, an integrated reverse osmosis device is used, and the RO high-pressure pump adopts a vertical multi-stage stainless steel pump with a flow rate of ≧36m 3 / h, head ≧140m, material 300 stainless steel, protection level not less than IP55, the start and stop of the water pump is interlocked with the liquid level signal of each process water tank, the pump inlet and outlet are installed with manual valves, and the outlet is installed with a check valve and a pressure gauge. It can also be manually operated on site. When in use, the prepared biofilm is placed in the sewage treatment pool. The sewage stays in the pool for not less than 8 hours to ensure that the microorganisms have enough time to fully contact and react with the pollutants in the sewage. At the same time, it is necessary to ensure that the contact area between the biofilm and the sewage is not less than 30 square meters to provide sufficient space for the action of microorganisms. During the treatment process, the dissolved oxygen concentration in the pool must be maintained at 2-5mg / L to provide space for microorganisms. In order to create a suitable living and metabolic environment for organisms, during the entire treatment process, the startup, operation, flushing and other processes of the reverse osmosis system can be automatically controlled. The design flux of the RO membrane element is not greater than the maximum flux value specified in the "Guidelines" of the membrane element manufacturer, and reasonable arrangements and combinations are selected to ensure the normal operation of the membrane element and a reasonable cleaning cycle. The control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated into the panel, which is easy to operate and use, greatly improving the efficiency and stability of sewage treatment. Through such a series of carefully designed and strictly controlled operating processes, a more efficient, stable and environmentally friendly sewage treatment effect can be achieved to meet increasingly stringent environmental protection requirements.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A high-efficiency sewage treatment biofilm for a sewage station, characterized in that: The biofilm contains nitrifying bacteria, denitrifying bacteria and bacillus, and the ratio of each microorganism is 3:2:

1.

2. A high-efficiency sewage treatment biofilm for a sewage station according to claim 1, characterized in that: The biofilm also contains activated carbon and zeolite powder, the contents of which are 15% and 10% respectively.

3. The preparation technology of a high-efficiency sewage treatment biofilm for a sewage station according to claim 2 is characterized in that: The following steps are involved: Step 1: Cultivate nitrifying bacteria, denitrifying bacteria and Bacillus in beef extract peptone medium, LB medium and nutrient broth medium respectively, the culture conditions are temperature 30°C, pH value 7.0, culture time is 48 hours, and ammonium chloride is added during the culture of nitrifying bacteria, the addition amount is 5g / L; Step 2: Mix the cultured microorganisms in a ratio of 3:2:1; Step 3: Add activated carbon and zeolite powder to the mixed microorganisms and stir evenly at 200 r / min; Step 4: The uniformly stirred mixture is formed into a biofilm under molding conditions of 5 MPa and 40° C., and the molding time is maintained for 2 hours.

4. The high-efficiency sewage treatment system for a sewage station according to claim 3 is characterized in that: Including the reverse osmosis system using an integrated reverse osmosis device, the equipment structure is compact and reasonable, and the processing capacity of a single set is ≧25m 3 / h, single RO membrane water output ≧1.0t / h, single RO membrane desalination rate >99.5%, reverse osmosis membrane uses dry membrane. Control device, pressure gauge, online conductivity meter, flow meter, etc. are integrated on the panel for easy operation.

5. The high-efficiency sewage treatment system for a sewage station according to claim 4 is characterized in that: The RO high-pressure pump adopts a vertical multi-stage stainless steel pump. The start and stop of the water pump is interlocked with the liquid level signal of each process water tank. Manual valves are installed at the pump inlet and outlet, and a check valve and pressure gauge are installed at the outlet. Manual operation is also possible on site. Water pump flow ≧36m 3 / h, head ≧140m, material 304 stainless steel, protection grade not less than IP55.

6. A water station efficient sewage treatment system according to claim 5, characterized in that: The startup, operation, flushing and other processes of the reverse osmosis system can be automatically controlled. The design flux of the RO membrane element is not greater than the maximum flux value specified in the "Guidelines" of the membrane element manufacturer, and a reasonable arrangement and combination is selected to ensure the normal operation of the membrane element and a reasonable cleaning cycle.

7. The method for using a high-efficiency sewage treatment biofilm in a sewage station according to claim 6, characterized in that: The biofilm is placed in a sewage treatment pool, the sewage stays in the pool for no less than 8 hours, the contact area between the biofilm and the sewage is no less than 30 square meters, and the dissolved oxygen concentration in the pool is maintained at 2-5 mg / L during the treatment process.