Edible and medicinal mushroom research and development strain breeding and preservation device and preservation method thereof

Abstract

The application belongs to the technical field of strain breeding and preservation devices, and discloses a strain breeding and preservation device for edible and medicinal mushroom research and development and a preservation method thereof, which comprises a device main body, a box door hinged to one side of the device main body, a storage rack installed in the device main body, and a ventilation assembly arranged in the device main body. The ventilation assembly comprises a fan frame arranged in the device main body, a filter assembly arranged at the top end of the fan frame, and a ventilation fan installed in the fan frame. A carbon dioxide monitor is installed on the sidewall of the device main body. When cultivating ganoderma lucidum strains, first, inoculate the ganoderma lucidum seed liquid into the culture medium, then place it on the top end of the storage rack, and finally place the storage rack in the device main body for cultivation. When cultivating, the carbon dioxide concentration in the device main body is monitored in real time by the carbon dioxide monitor. When the monitored carbon dioxide concentration reaches a certain threshold, the ventilation fan is started to ventilate through PLC control, thereby improving the growth efficiency and quality.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of strain breeding and preservation devices, in particular to a strain breeding and preservation device for edible and medicinal mushroom research and development and a preservation method thereof. BACKGROUND

[0002] Edible and medicinal mushrooms are a large category of fungi with edible and medicinal functions. They are on a par with the plant kingdom and the animal kingdom in nature, have extremely high biological diversity and important economic value. In the research and development process of edible and medicinal mushrooms, strains are the basis and core. In order to ensure the purity, activity and genetic stability of the strains, the strains must be properly preserved and managed.

[0003] After searching, the patent CN115627235A discloses a freeze-drying preservation method for ganoderma lucidum mycelium, which comprises the following steps: (1) inoculating ganoderma lucidum seed liquid into culture medium, shaking bed culture, adding sterilized polyvinylpyrrolidone at a final concentration of 0.6% when cultured to the fifth day, and continuing to culture to the seventh day; (2) adding sterile skimmed milk powder to the ganoderma lucidum seed liquid to be freeze-dried at a final concentration of 10%, placing it in a 4°C refrigerator overnight, and adding sterile-treated trehalose and mannitol to the ganoderma lucidum seed liquid after standing overnight and shaking well; (3) placing the ganoderma lucidum seed liquid obtained in step (2) in a -20°C refrigerator first, then transferring it to a freeze dryer, and freeze-drying to obtain ganoderma lucidum mycelium freeze-dried powder. The ganoderma lucidum mycelium is freeze-dried, and the seed activity remains unchanged after being stored at room temperature for half a year.

[0004] In the current breeding process of ganoderma lucidum strains, the shaking bed culture method is usually used. This culture method needs to be ventilated regularly during the culture process to ensure that ganoderma lucidum can grow in a suitable environment. Although the current shaking bed equipment is equipped with a ventilation structure, the ventilation frequency of the current ventilation structure is difficult to adjust according to the growth stage of the ganoderma lucidum strains because the culture period of the shaking bed is relatively long and the ventilation time required by the ganoderma lucidum strains is inconsistent at different growth stages, which affects the quality and efficiency of the culture of the ganoderma lucidum strains to some extent. SUMMARY

[0005] The purpose of the present application is to provide a strain breeding and preservation device for edible and medicinal mushroom research and development and a preservation method thereof to solve the problems raised in the background.

[0006] In order to achieve the above-mentioned purpose, the present application provides the following technical scheme: a strain breeding and preservation device for edible and medicinal mushroom research and development, comprising a device main body, a box door hinged to one side of the device main body, a storage rack installed in the interior of the device main body, and a ventilation assembly arranged in the interior of the device main body.

[0007] The ventilation assembly comprises a fan frame arranged inside the device body, the top end of the fan frame is provided with a filter assembly, the inside of the fan frame is provided with a ventilation fan, the four corners of the fan frame are fixedly connected with first connecting rods, the bottom end of the four first connecting rods is provided with a mounting seat, each first connecting rod and mounting seat are connected through connecting bolts, each mounting seat is fixedly installed in the inside of the device body, the inside of the device body is provided with a shunt hole and an air outlet hole, the shunt hole is arranged above the shelf, and the sidewall of the device body is provided with a carbon dioxide monitor.

[0008] As a further technical scheme of the present application, the filter assembly comprises a filter plate arranged at the top end of the fan frame, the inside of the filter plate is uniformly provided with filter holes, the four corners of the filter plate are fixedly connected with second connecting rods, the second connecting rods are arranged at the top end of the first connecting rods, and a connecting assembly is arranged between the second connecting rods and the first connecting rods.

[0009] As a further technical scheme of the present application, the bottom of the fan frame is provided with a second filter screen, and the second filter screen is installed in the inside of the device body.

[0010] As a further technical scheme of the present application, the inside of the air outlet hole is installed with a first filter screen.

[0011] As a further technical scheme of the present application, the connecting assembly comprises a wedge block embedded in the inside of the first connecting rod, one end of the wedge block is fixedly connected with a first connecting plate, the first connecting plate is slidingly installed in the inside of the second connecting rod, and one side of the first connecting plate is provided with a spring.

[0012] As a further technical scheme of the present application, the top end of the first connecting plate is fixedly connected with a second connecting plate, the top end of the second connecting plate is fixedly connected with a moving plate, and the moving plate is arranged at the top end of the filter plate.

[0013] As a further technical scheme of the present application, the inner wall of the filter plate is embedded with a connecting pipe, the outer wall of the bottom end of the connecting pipe is fixedly connected with a fixed rod, the fixed rod is fixedly installed on the inner wall of the filter hole, and the top end of the connecting pipe is fixedly connected with a demonstration belt.

[0014] As a further technical scheme of the present application, the demonstration belt is arranged at the top end of the filter plate.

[0015] As a further technical scheme of the present application, the top end of the demonstration belt is provided with an exhaust hole.

[0016] A preservation method of a fungus breeding and preservation device for food and medicinal fungus research and development, comprising the following steps:

[0017] S1: First, the ganoderma seed liquid is inoculated in the culture medium, placed at the top end of the shelf, and then placed in the device main body for culture, and on the fifth day of culture, the sterilized polyvinylpyrrolidone is added at a final concentration of 0.6%, and then the culture is continued to the seventh day;

[0018] S2: The ganoderma seed liquid to be freeze-dried is added with sterile skimmed milk powder at a final concentration of 10%, and then placed in a 4℃ refrigerator overnight, and then the sterile treated trehalose and mannitol are added to the ganoderma seed liquid after standing overnight, and then shaken well;

[0019] S3: The obtained ganoderma seed liquid is first placed in a-20℃ refrigerator, and then transferred to a freeze dryer for freeze-drying treatment to obtain ganoderma mycelium freeze-dried powder;

[0020] S1 further comprises:

[0021] S11: During the culture process, the carbon dioxide monitor is used to monitor the carbon dioxide concentration in the device main body in real time, and when the monitored carbon dioxide concentration reaches a certain threshold value, the air inlet fan is started by PLC control to make the external air enter;

[0022] S12: The external air is first filtered by the filter plate and the filter hole, and then flows downward to contact the second filter screen, and then filtered again by the second filter screen, and then guided to the side wall of the device main body by the shunt hole, so as to ventilate and exchange air in the device main body;

[0023] S13: When the external air enters the inside of the filter hole, part of the gas flows upward to the inside of the connection pipe and the indication belt, the gas pressure in the inside of the connection pipe and the indication belt increases until the inside of the indication belt is filled with gas to make the indication belt expand and stretch, so as to facilitate the maintenance of the filter plate by the staff.

[0024] The beneficial effects of the present application are as follows:

[0025] By arranging the ventilation assembly, when the ganoderma seed is cultured, the ganoderma seed liquid is first inoculated in the culture medium, and then placed at the top end of the shelf, and then placed in the device main body for culture, and during the culture, the carbon dioxide monitor is used to monitor the carbon dioxide concentration in the device main body in real time, and when the monitored carbon dioxide concentration reaches a certain threshold value, the air inlet fan is started by PLC control to make the external air enter and be discharged to the device main body through the shunt hole for ventilation, so as to reduce the carbon dioxide concentration and provide a suitable and stable growth environment for the cultivation of the ganoderma seed, thereby effectively improving the growth efficiency and quality.

[0026] By setting up a filtration component, when the equipment starts the ventilation fan, the outside air is first filtered by the filter plate before entering the equipment. The gas filtration by the filter plate can minimize the possibility of external pollutants entering the main body of the device, avoid contamination by miscellaneous bacteria, and improve the cultivation environment.

[0027] This invention, through the configuration of the connecting components, allows the filter plate to be placed at the top of the fan frame during installation of the filter plate and fan frame. This enables the second connecting rod to be embedded inside the first connecting rod. When the second connecting rod is embedded in the first connecting rod, the wedge is embedded inside the second connecting rod. Finally, the connecting bolt passes through the inner walls of the second and first connecting rods and is embedded into the mounting base. Finally, the connecting bolt is rotated to install the fan frame inside the mounting base. During maintenance, the fan frame can be pulled upwards together by the limiting position of the wedge, facilitating maintenance. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 This is a schematic cross-sectional view of the overall structure of the present invention;

[0030] Figure 3 This is a schematic diagram of the structure of the mounting base of the present invention;

[0031] Figure 4 This is a schematic diagram of the filter plate structure of the present invention;

[0032] Figure 5 This is a schematic cross-sectional view of the filter plate structure of the present invention;

[0033] Figure 6 For the present invention Figure 5 Enlarged schematic diagram of the structure at point A in the middle;

[0034] Figure 7 This is a schematic cross-sectional view of the filter hole structure of the present invention;

[0035] Figure 8 For the present invention Figure 7 Enlarged schematic diagram of the structure at point B;

[0036] In the diagram: 1. Main body of the device; 2. Door; 3. Shelf; 4. Fan frame; 5. Ventilation fan; 6. First connecting rod; 7. Mounting base; 8. Connecting bolt; 9. Diverter hole; 10. Carbon dioxide monitor; 11. Exhaust port; 12. First filter screen; 13. Filter plate; 14. Filter hole; 15. Second connecting rod; 16. Second filter screen; 17. First connecting plate; 18. Wedge block; 19. Spring; 20. Second connecting plate; 21. Moving plate; 22. Connecting pipe; 23. Fixing rod; 24. Schematic strip; 25. Exhaust port. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] like Figures 1 to 8 As shown in the embodiment of the present invention, a breeding and preservation device for edible and medicinal fungi research includes a device body 1, a door 2 hinged to one side of the device body 1, a shelf 3 installed inside the device body 1, and a ventilation component installed inside the device body 1.

[0039] The ventilation assembly includes a fan frame 4 installed inside the main body 1 of the device. A filter assembly is installed at the top of the fan frame 4. An exhaust fan 5 is installed inside the fan frame 4. First connecting rods 6 are fixedly connected to the four corners of the fan frame 4. Mounting seats 7 are installed at the bottom of the four first connecting rods 6. Each first connecting rod 6 is connected to the mounting seat 7 by connecting bolts 8. Each mounting seat 7 is fixedly installed inside the main body 1 of the device. A diversion hole 9 and an air outlet 11 are opened inside the main body 1 of the device. The diversion hole 9 is located above the shelf 3. A carbon dioxide monitor 10 is installed on the side wall of the main body 1 of the device.

[0040] When cultivating Ganoderma lucidum spores, the Ganoderma lucidum seed liquid is first inoculated into the culture medium, then placed on top of the shelf 3, and then the shelf 3 is placed inside the main body 1 of the device for cultivation. During cultivation, the carbon dioxide concentration inside the main body 1 of the device is monitored in real time by the carbon dioxide monitor 10. When the monitored carbon dioxide concentration reaches a certain threshold (which is preset), the ventilation fan 5 is started by controlling the PLC to allow external air to enter and be discharged into the main body 1 of the device through the diversion hole 9 for ventilation, so as to reduce the carbon dioxide concentration and provide a suitable and stable growth environment for the cultivation of Ganoderma lucidum spores, thereby effectively improving its growth efficiency and quality.

[0041] The device body 1 has evenly distributed diversion holes 9 on all four sides inside, and the diversion holes 9 are set downward so that when the gas enters, it blows towards the side wall of the device body 1, avoiding the wind from blowing directly onto the culture placed at the top of the shelf 3, preventing the mycelium from drying out or being mechanically damaged, thereby ensuring the breeding quality.

[0042] Mounting base 7 and connecting bolt 8 are connected by internal and external threads;

[0043] The ventilation fan 5 has a common structure, and its working method will not be described in detail here.

[0044] The method for obtaining the ganoderma lucidum seed liquid is as follows: the original seed is expanded in tissue, the expanded ganoderma lucidum seed is inoculated into a liquid culture medium, and is placed in a 28 DEG C constant temperature box, and is cultured on a shaking table for 3 days at a temperature of 27 DEG C and a rotating speed of 160 rpm / min; the liquid culture medium contains 50 g of yeast extract powder, 3 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, 20 g of glucose and 60 g of protein peptone per 1000 ml;

[0045] The liquid culture medium contains 50 g of yeast extract powder, 3 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, 20 g of glucose, 60 g of protein peptone and 25 g of wood chips per 1000 ml.

[0046] As shown in Figures 1 to 8 , the filter plate 13 is arranged at the top end of the fan frame 4, the filter plate 13 is uniformly provided with filter holes 14 in the inside, the second connecting rod 15 is fixedly connected at the four corners of the filter plate 13, the second connecting rod 15 is arranged at the top end of the first connecting rod 6, and the connecting assembly is arranged between the second connecting rod 15 and the first connecting rod 6.

[0047] When the device starts the ventilation fan 5 to ventilate, the external air is filtered by the filter plate 13 before entering the device, the gas is filtered by the filter plate 13, the possibility of external pollutants entering the device main body 1 is reduced as much as possible, the contamination of miscellaneous bacteria is avoided, and the culture environment is improved.

[0048] As shown in Figure 3 , the second filter screen 16 is arranged below the fan frame 4, and the second filter screen 16 is arranged in the inside of the device main body 1.

[0049] When the device ventilates, the external air is filtered by the filter plate 13 and then flows downward to contact the second filter screen 16, the second filter screen 16 is used for filtering again, and the culture environment in the inside of the device main body 1 is further ensured, so that the breeding quality of the strain is ensured.

[0050] The second filter screen 16 is in sliding connection with the mounting seat 7, when the second filter screen 16 is maintained, the fan frame 4 and the filter plate 13 are first disassembled, and then the second filter screen 16 is directly taken out upward for maintenance.

[0051] As shown in Figure 2 , the first filter screen 12 is arranged in the inside of the air outlet hole 11.

[0052] The possibility of the external impurities entering the inside of the device from the air outlet hole 11 is reduced as much as possible.

[0053] As shown in Figure 5 and Figure 6 , the connecting assembly comprises a wedge block 18 embedded in the inside of the first connecting rod 6, one end of the wedge block 18 is fixedly connected with a first connecting plate 17, the first connecting plate 17 is in sliding connection in the inside of the second connecting rod 15, and the first connecting plate 17 is provided with a spring 19 on one side.

[0054] In the installation filter plate 13 and fan frame 4, first filter plate 13 is placed in the top end of the fan frame 4, the second connecting rod 15 embedded in the first connecting rod 6 inside, the second connecting rod 15 embedded in the first connecting rod 6 when the wedge block 18 bottom end of the inclined surface and the outer wall of the first connecting rod 6 contact force slip into the second connecting rod 15 inside, and make the spring 19 deformation storage elastic potential energy;

[0055] The first connecting rod 6 is provided with a sliding groove for the wedge block 18 to slide, when the wedge block 18 moves to one side of the sliding groove, the spring 19 is released to push the wedge block 18 to move outward and embed in the sliding groove, so as to connect the second connecting rod 15 with the first connecting rod 6, and finally through the connecting bolt 8 passing through the inner wall of the second connecting rod 15 and the first connecting rod 6 and embedding into the mounting seat 7, finally through the rotating connecting bolt 8 to install it in the inside of the mounting seat 7;

[0056] When the fan frame 4 needs to be maintained, first rotate the connecting bolt 8 to remove it, then pull up the filter plate 13, the fan frame 4 can be pulled out upward through the limiting of the wedge block 18, which is convenient for maintenance.

[0057] As shown in Figure 6 The top end of the first connecting plate 17 is fixedly connected with the second connecting plate 20, the top end of the second connecting plate 20 is fixedly connected with the moving plate 21, and the moving plate 21 is arranged at the top end of the filter plate 13.

[0058] When the filter plate 13 and the fan frame 4 need to be separated, the moving plate 21 is moved to one side, the movement of the moving plate 21 drives the movement of the second connecting plate 20, the movement of the second connecting plate 20 drives the movement of the first connecting plate 17, and the movement of the first connecting plate 17 drives the wedge block 18 to slide out of the first connecting rod 6, so as to facilitate the upward movement of the filter plate 13 and the separation from the fan frame 4.

[0059] As shown in Figure 7 And Figure 8 The inner wall of the filter plate 13 is embedded with the connecting pipe 22, the outer wall of the bottom end of the connecting pipe 22 is fixedly connected with the fixed rod 23, the fixed rod 23 is fixedly installed on the inner wall of the filter hole 14, and the top end of the connecting pipe 22 is fixedly connected with the indicating belt 24.

[0060] The inner wall of part of the filter hole 14 is provided with the connecting pipe 22 through the fixed rod 23;

[0061] The indicating belt 24 is made of non-breathable material, such as plastic and rubber, which can be inflated to expand and show.

[0062] Through the setting of the connecting pipe 22, when ventilating, air enters the inside of the filter hole 14, part of the gas flows into the inside of the connecting pipe 22 to the inside of the demonstration belt 24 upwards, the air pressure in the inside of the connecting pipe 22 and the demonstration belt 24 increases until the inside of the demonstration belt 24 is full of gas to make the demonstration belt 24 expand and stretch, at this time, the worker can see that the demonstration belt 24 changes, and knows that the gas in the inside of the filter hole 14 flows, and the filter plate 13 does not need to be maintained.

[0063] When the inside of the filter hole 14 accumulates some impurities with the increase of the use time, the inside of the filter hole 14 is blocked or the gas flow effect is poor, at this time, the air pressure in the inside of the connecting pipe 22 is not enough, the demonstration belt 24 cannot stretch or the effect of the expansion and stretching is not enough, the worker can observe that the filter plate 13 needs to be maintained, so as to maintain the filter plate 13 in time, ensure the sterile environment in the inside of the device main body 1 to breed the strain, and thus improve the strain breeding quality when the edible and medicinal fungus is researched and developed.

[0064] As shown in Figure 7 , the demonstration belt 24 is arranged at the top end of the filter plate 13.

[0065] As shown in Figure 8 , the top end of the demonstration belt 24 is provided with an exhaust hole 25.

[0066] When ventilating, the inside of the demonstration belt 24 always has gas entering, part of the gas can be discharged through the exhaust hole 25, so as to ensure the stability and reliability of the demonstration belt 24.

[0067] A preservation method of a strain breeding and preservation device for edible and medicinal fungus research and development, comprising the following steps:

[0068] S1: first inoculate ganoderma lucidum seed liquid into culture medium, place the culture medium at the top end of the shelf 3, and then place the shelf 3 in the device main body 1 for culture, when the culture is carried out for five days, add sterilized polyvinylpyrrolidone with a final concentration of 0.6%, and then continue to culture until the seventh day;

[0069] S2: add sterile skimmed milk powder to the ganoderma lucidum seed liquid to be freeze-dried with a final concentration of 10%, and then place the mixture in a 4℃ refrigerator overnight, add sterile treated trehalose and mannitol to the ganoderma lucidum seed liquid after standing overnight, and shake well;

[0070] S3: place the obtained ganoderma lucidum seed liquid in a-20℃ refrigerator first, and then transfer it to a freeze dryer for freeze-drying treatment to obtain ganoderma lucidum mycelium freeze-dried powder;

[0071] S1 further comprises:

[0072] S11: the carbon dioxide concentration inside the device main body 1 is monitored in real time by the carbon dioxide monitor 10 during the culture process, when the monitored carbon dioxide concentration reaches a certain threshold, the air exchange fan 5 is started by the PLC control to make the external air enter;

[0073] S12: the external air flows downward after being filtered by the filter plate 13 and the filter hole 14 and contacts the second filter screen 16, is filtered again by the second filter screen 16, is guided to the side wall of the device main body 1 by the shunt hole 9, and thus the device main body 1 is ventilated;

[0074] S13: when the external air enters the inside of the filter hole 14, part of the gas flows upward to the schematic belt 24 inside the connecting pipe 22, the air pressure inside the connecting pipe 22 and the schematic belt 24 increases until the schematic belt 24 is full of gas to make the schematic belt 24 expand and stretch, which is convenient for the staff to observe and maintain the filter plate 13.

[0075] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A breeding and preservation device for edible and medicinal fungi, comprising a main body (1), characterized in that: A door (2) is hinged to one side of the main body (1) of the device, a shelf (3) is installed inside the main body (1), and a ventilation component is provided inside the main body (1); The ventilation assembly includes a fan frame (4) installed inside the main body (1) of the device. A filter assembly is installed at the top of the fan frame (4). An air exchange fan (5) is installed inside the fan frame (4). First connecting rods (6) are fixedly connected to the four corners of the fan frame (4). Mounting seats (7) are provided at the bottom of the four first connecting rods (6). Each first connecting rod (6) is connected to the mounting seat (7) by connecting bolts (8). Each mounting seat (7) is fixedly installed inside the main body (1). A diversion hole (9) and an air outlet (11) are opened inside the main body (1). The diversion hole (9) is located above the shelf (3). A carbon dioxide monitor (10) is installed on the side wall of the main body (1). The filter assembly includes a filter plate (13) set at the top of the fan frame (4), the filter plate (13) has filter holes (14) evenly opened inside, and a second connecting rod (15) is fixedly connected to each of the four corners of the filter plate (13). The second connecting rod (15) is set at the top of the first connecting rod (6), and a connecting assembly is provided between the second connecting rod (15) and the first connecting rod (6). The connecting assembly includes a wedge (18) embedded inside the first connecting rod (6), one end of the wedge (18) is fixedly connected to a first connecting plate (17), the first connecting plate (17) is slidably installed inside the second connecting rod (15), and a spring (19) is provided on one side of the first connecting plate (17). The top end of the first connecting plate (17) is fixedly connected to a second connecting plate (20), and the top end of the second connecting plate (20) is fixedly connected to a movable plate (21), which is disposed at the top end of the filter plate (13); The inner wall of the filter plate (13) is embedded with a connecting tube (22), and a fixing rod (23) is fixedly connected to the outer wall of the bottom end of the connecting tube (22). The fixing rod (23) is fixedly installed on the inner wall of the filter hole (14), and a schematic strip (24) is fixedly connected to the top end of the connecting tube (22).

2. The breeding and preservation device for edible and medicinal fungi research and development as described in claim 1, characterized in that: A second filter (16) is provided below the fan frame (4), and the second filter (16) is installed inside the main body (1) of the device.

3. The breeding and preservation device for edible and medicinal fungi research and development as described in claim 2, characterized in that: The air outlet (11) is equipped with a first filter screen (12).

4. The breeding and preservation device for edible and medicinal fungi research and development as described in claim 3, characterized in that: The schematic strip (24) is set at the top of the filter plate (13).

5. The breeding and preservation device for edible and medicinal fungi research and development according to claim 4, characterized in that: The top of the schematic strip (24) is provided with an exhaust hole (25).

6. A method for preserving edible and medicinal fungi strains for research and development using a preservation device, wherein the method uses the edible and medicinal fungi strains for research and development preservation device as described in claim 5, characterized in that, Includes the following steps: S1: First, inoculate the Ganoderma lucidum seed liquid into the culture medium and place it on the top of the shelf (3). Then place the shelf (3) in the main body (1) of the device for cultivation. On the fifth day of cultivation, add sterilized polyvinylpyrrolidone at a final concentration of 0.6% and continue to cultivate until the seventh day to end the cultivation. S2: Add the freeze-dried Ganoderma lucidum seed liquid to sterile skim milk powder at a final concentration of 10%, and let it stand overnight in a 4°C refrigerator. Add the sterile trehalose and mannitol to the Ganoderma lucidum seed liquid after it has stood overnight, and shake well. S3: The obtained Ganoderma lucidum seed liquid was first placed in a -20℃ refrigerator, and then transferred to a freeze dryer for freeze drying to obtain Ganoderma lucidum mycelium freeze-dried powder. S1 also includes: S11: During the cultivation process, the carbon dioxide concentration inside the main body (1) of the device is monitored in real time by a carbon dioxide monitor (10). When the monitored carbon dioxide concentration reaches a certain threshold, the ventilation fan (5) is started by controlling the PLC to allow external air to enter. S12: External air is first filtered by the filter plate (13) and filter holes (14) and then flows downward to contact the second filter screen (16). It is filtered again by the second filter screen (16) and then guided by the diversion hole (9) to spray onto the side wall of the device body (1), thereby ventilating the device body (1). S13: When external air enters the filter hole (14), some gas enters the interior of the connecting pipe (22) and flows upward to the schematic strip (24). The air pressure inside the connecting pipe (22) and the schematic strip (24) increases until the schematic strip (24) is filled with gas, causing the schematic strip (24) to expand and stretch, making it easier for staff to observe and maintain the filter plate (13).

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