Bactericidal composition containing Aminopyrifen and pydiflumetofen and application thereof

Abstract

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing Aminopyrifen and pydiflumetofen and application of the bactericidal composition, and the mass ratio of Aminopyrifen to pydiflumetofen in active ingredients of the bactericidal composition is (1: 3)-(2: 3); the bactericidal composition has an excellent bactericidal effect, has a remarkable synergistic effect on wheat scab, can effectively reduce the use dosage of active ingredients and slow down the generation of drug resistance of germs, and is safe to crops.

Description

Technical Field

[0001] The invention belongs to the field of agricultural fungicides, and particularly relates to a fungicide composition containing Aminopyrifen and pentopyraclostrobin and application thereof. Background Art

[0002] Wheat fusarium head blight, caused by the Fusarium graminearum species complex (FGSC), is an important disease worldwide, usually causing a 10%-20% reduction in wheat yields. In severe cases, wheat yields can be reduced by 80%-90% or even total crop failure. In 1996, the International Maize and Wheat Improvement Center (CIMMYT) in Mexico identified fusarium head blight as one of the main factors restricting wheat yields. In recent years, due to changes in farming systems such as returning straw to the field and the promotion and application of no-till technology, wheat head blight has become more severe in my country, with frequent epidemics and an increasing area of ​​disease. It has spread from the provinces of frequent and repeated outbreaks in the middle and lower reaches of the Yangtze River to the main wheat producing areas of Shandong, Henan, and Hebei in the north, causing huge yield and economic losses. In addition to causing serious grain production losses, wheat fusarium fusarium rust is also more serious because the pathogen produces fungal toxins in the diseased grains, such as deoxynivalenol (DON). This toxin has strong cytotoxicity and embryotoxicity, and can cause esophageal cancer, IgA nephropathy, and miscarriage in pregnant women.

[0003] There are two main problems in the prevention and control of wheat fusarium head blight in current production: (1) there is a lack of wheat varieties that are highly resistant to fusarium head blight, and the main reliance on chemical agents for prevention and control; (2) there is a shortage of chemical control agents, and the problem of pathogen resistance is prominent. Therefore, the development of green and efficient disease prevention combination agents is of great practical significance for the sustainable prevention and control of wheat fusarium head blight.

[0004] Aminopyrifen belongs to the pyridine fungicide class and has a broad-spectrum, highly effective fungicide activity. Its chemical name is: (4-phenoxyphenyl)methyl-2-amino-6-methyl-3-pyridinecarboxylate. The fungicidal mechanism of aminopyrifen is unique and novel. As an inhibitor of the acyltransferase GWT-1, it blocks the biosynthesis of glycosylphosphatidylinositol (GPI) precursor anchor protein, thereby terminating the growth and reproduction of fungi. It has good preventive effects on a variety of plant diseases, such as gray mold, powdery mildew, sclerotinia, anthracnose, apple scab and rice blast. The chemical structure of aminopyrifen is as follows:

[0005]

[0006] Fluopicolide is a fungicide belonging to the class of succinate dehydrogenase inhibitors, with the chemical name: 3-(difluoromethyl)-N-methoxy-1-methyl-N-[(RS)-1-methyl-2-(2,4,6-trichlorophenyl)ethyl]-1H-pyrazole-4-carboxamide. The agent targets the succinate dehydrogenase in the respiratory electron transport chain complex II, interferes with the tricarboxylic acid cycle and destroys the function of mitochondria, prevents pathogens from producing energy, inhibits the growth and development of pathogens, and ultimately achieves the effect of sterilization and disease prevention. Fluopicolide has the characteristics of broad spectrum, high efficiency, and systemic absorption. It can be used to prevent and control diseases caused by a variety of pathogens such as Fusarium, powdery mildew, Botrytis cinerea, Alternaria alternata, and Pyricularia cinerea. It can be used not only on staple crops such as wheat, rice, and corn, but also on economic crops such as citrus, apples, and strawberries. Due to the single action site of fluopicolide, the risk of resistance is increasing with its widespread use. According to relevant research reports, field wheat fusarium resistant to fluopicolide has been found in my country [see the literature: Wenyong Shao, et al. Pest Management Science, 2022, 78(5): 1780-1788]. Therefore, the development of a suitable combination of agents is particularly important for crop disease prevention and control. The chemical structure of fluopicolide is as follows:

[0007]

[0008] Aminopyrifen and fluopicolide are both highly effective, low-toxic, and environmentally friendly fungicides. Aminopyrifen acts on the acyltransferase (GWT-1) of fungi, and fluopicolide acts on the succinate dehydrogenase of the fungus, thereby achieving the purpose of sterilization and prevention of plant diseases.

[0009] The International Fungicide Resistance Action Committee (FRAC) recommends that the combination of agents with different mechanisms of action can avoid or delay the development of resistance. The development of a fungicide combination of Aminopyrifen and fluopicolide can not only effectively solve the problem of crop disease prevention and control, but also avoid or delay the development of resistance by pathogens. So far, there have been no reports on the combination of Aminopyrifen and fluopicolide for disease prevention and control. Summary of the invention

[0010] The present invention aims to provide a highly efficient, low-toxic, environmentally friendly fungicide composition, which has excellent control effect on wheat scab, and can also control the production of drug resistance in pathogens and extend the service life of the agent. The specific technical scheme is as follows:

[0011] A fungicide composition containing aminopyrifen and fluopyram, wherein the active ingredients are composed of aminopyrifen and fluopyram, and the mass ratio of aminopyrifen and fluopyram is (1:3)-(2:3). The preferred mass ratio of aminopyrifen and fluopyram is 1:3 or 2:3. The more preferred mass ratio of aminopyrifen and fluopyram is 1:3.

[0012] The bactericidal composition of the present invention further comprises agricultural auxiliary components and is configured into a pesticide preparation. The total mass percentage of the active components is 5%-75%. The preferred total mass percentage of the active components is 10%-60%.

[0013] The bactericidal composition preparation of the present invention is a suspension, microemulsion, aqueous emulsion, wettable powder, water-dispersible granule or dispersible oil suspension, preferably a suspension or water-dispersible granule.

[0014] The fungicide composition of the present invention is used for preventing and controlling crop diseases, and preferably the crop disease is wheat fusarium head blight.

[0015] Compared with the prior art, the present invention achieves the following excellent technical effects:

[0016] The bactericidal composition of the present invention has an excellent control effect on wheat scab, and can also control the generation of drug resistance of pathogenic bacteria and prolong the service life of the agent.

[0017] When the mass ratio of Aminopyrifen and fluopicolide is (1:3)-(2:3), a significant synergistic effect is exhibited on wheat fusarium head blight, which can effectively reduce the dosage of active ingredients and is safe for crops.

[0018] Specific embodiment

[0019] The following examples are only used to illustrate the present invention and are not intended to limit the scope of application of the present invention. Without departing from the spirit and essence of the present invention, any modification or replacement of the methods, steps or conditions of the present invention shall fall within the scope of the present invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified. Example 1. Indoor toxicity determination of the combination of Aminopyrifen and Fluopyram against wheat fusarium

[0020] Test object: wheat fusarium (Fusarium graminearum), which was isolated and purified from wheat ears with natural fusarium graminearum in the field. After the strain was identified by morphology and pathogenicity, it was prepared into 15% glycerol bacteria and frozen in an ultra-low temperature refrigerator for use.

[0021] Test method: refer to the mycelium growth rate method in the "People's Republic of China Agricultural Industry Standard NY / T 1156.6-2006 Pesticide Indoor Bioassay Test Standard for Fungicides Part 6: Determination of the Combined Effect of Mixtures". Each single agent and each group of mixed agents are set at 5 mass concentrations according to the content of the active ingredient, and the inhibition rate of the growth of the pathogen is between 10% and 95%. The wheat fusarium in the frozen tube of the strain is activated on the PDA culture medium. When the colony size is about 4 / 5 of the culture dish, a sterilized stainless steel puncher is used to punch a 5mm diameter bacterial cake from the edge of the colony. The sterile inoculation needle transfers the bacterial cake to the center of the pre-prepared series of concentrations of drug-containing YBA plates, and the plates are placed in an incubator with a temperature of 25℃ and a photoperiod of 12L:12D and inverted for 3 days. The diameter of the colony is measured by the cross method to calculate the corrected inhibition percentage; then the EC of each agent is calculated based on the linear regression equation between the probability value of the inhibition rate and the logarithm of the series of concentrations. 50 Each treatment was repeated 4 times, and a treatment without drug was set as a blank control. The synergistic coefficient (SR) was calculated according to the Wadley method, and a comprehensive evaluation of the mixing effect was made. The SR value was calculated according to the following formula: EC 50 (Theoretical value) = (P A +P B ) / (P A / EC 50 A+P B / EC 50 B)

[0022] SR=EC 50 (Theoretical value) / EC 50 (Actual value)

[0023] Among them, P A is the percentage of drug A in the mixture, P B It is the percentage of agent B in the mixture.

[0024] SR≤0.5 indicates an antagonistic effect, 0.5<SR<1.5 indicates an additive effect, and SR≥1.5 indicates a synergistic effect.

[0025] Test results: From the data in Table 1, it can be seen that both Aminopyrifen and fluopicolide have excellent antibacterial effects on wheat fusarium; the combination of Aminopyrifen and fluopicolide in ratios of 1:3 and 2:3 showed a synergistic effect, among which the maximum synergistic ratio was 1:3.

[0026] Table 1 Results of indoor toxicity test of Aminopyrifen and fluopyraclostrobin against wheat fusarium sphaeroides

[0027]

[0028]

[0029] Example 2. Field efficacy of the combination of Aminopyrifen and fluopicolide against wheat fusarium head blight

[0030] Control target: Wheat fusarium head blight

[0031] Test drug: All percentages in the preparation ratios are by mass.

[0032] (1) 20% Aminopyrifen suspension

[0033] Weigh 20% Aminopyrifen, 2% Morwet D-390, 3% TERSPERSE 2425, 3% Dispersol PSR-19, 0.1% xanthan gum, 5% polyethylene glycol 4000, 0.3% sodium benzoate, and 0.1% phosphate defoamer, and add deionized water to 100% by mass.

[0034] According to the formula ratio, with water as the medium, add the original drug, dispersant, antifreeze and defoamer into the batching kettle and mix evenly. Then, ball mill it for 30 minutes, pump it into the dispersion tank for high-speed shearing for 3 minutes, and grind it fully with a sand mill to make a suspension.

[0035] (2) 20% Aminopyrifen·Pyridoxal Suspension

[0036] Weigh 5% Aminopyrifen, 15% Fluopyram, 2% Morwet D-390, 3% TERSPERSE2425, 3% Dispersol PSR-19, 0.1% xanthan gum, 5% polyethylene glycol 4000, 0.3% sodium benzoate, and 0.1% phosphate defoamer, and add deionized water to 100% by mass.

[0037] According to the formula ratio, with water as the medium, add the original drug, dispersant, antifreeze and defoamer into the batching kettle and mix evenly. Then, ball mill it for 30 minutes, pump it into the dispersion tank for high-speed shearing for 3 minutes, and grind it fully with a sand mill to make a suspension.

[0038] (3) 20% Aminopyrifen·Pyridoxal Suspension

[0039] Weigh 5% Aminopyrifen, 15% Fluopyram, 4% Morwet D-390, 4% TERSPERSE 2425, 0.1% xanthan gum, 5% polyethylene glycol 4000, 0.3% sodium benzoate, and 0.1% phosphate defoamer, and add deionized water to 100% by mass.

[0040] According to the formula ratio, with water as the medium, add the original drug, dispersant, antifreeze and defoamer into the batching kettle and mix evenly. Then, ball mill it for 30 minutes, pump it into the dispersion tank for high-speed shearing for 3 minutes, and grind it fully with a sand mill to make a suspension.

[0041] (4) 20% Aminopyrifen·Pyridoxal Suspension

[0042] Weigh 5% Aminopyrifen, 15% Fluopyram, 2% calcium lignin sulfonate, 3% sodium lauryl sulfate, 3% Dispersol PSR-19, 0.1% xanthan gum, 5% polyethylene glycol 4000, 0.3% sodium benzoate, and 0.1% phosphate defoamer, and add deionized water to 100% by mass.

[0043] According to the formula ratio, with water as the medium, add the original drug, dispersant, antifreeze and defoamer into the batching kettle and mix evenly. Then, ball mill it for 30 minutes, pump it into the dispersion tank for high-speed shearing for 3 minutes, and grind it fully with a sand mill to make a suspension.

[0044] (5) 40% Aminopyrifen·Pyridoxal water dispersible granules

[0045] Weigh 10% Aminopyrifen, 30% Fluopyram, 4% lignin calcium sulfonate, 4% TERSPERSE 2700, 3% sodium dodecyl sulfate, 2% sodium carboxymethyl cellulose, 2% diatomaceous earth, 5% ammonium sulfate, and attapulgite and add the mixture to 100%.

[0046] According to the formula ratio, the original drug and dispersant, wetting agent, binder, etc. are mixed evenly, crushed by ultrafine air flow mill, granulated by granulator, and finally dried and sieved to obtain water-dispersible granules.

[0047] Test method: Modern agricultural science and technology demonstration park in Fengtai County, Anhui Province, wheat variety is Yannong 5286 (susceptible variety). According to the provisions of "Agricultural Industry Standard of the People's Republic of China NY / T 1464.15-2007 Pesticide Field Efficacy Test Guidelines Part 15: Fungicide Control of Wheat Fusarium Head Blight", the test was designed with 7 treatments, including 6 treatments and 1 blank control, each treatment was repeated 3 plots, each plot was 30m 2(3m×10m), the treatment groups were randomly arranged, and adjacent treatments were set up with 1m protection rows. The pesticide was applied once at the beginning of wheat heading and flowering, and then applied again after an interval of 7 days. The results were investigated 15 days after the application. Each experimental plot was sampled at 5 points on the diagonal line, and 100 ears were investigated at each point. The percentage of dead ear area to the entire wheat ear area was used for classification, and the number of diseased ears and the total number of ears at each level were recorded. The disease classification standards are as follows:

[0048] Level 0: The whole ear is disease-free;

[0049] Level 1: The dead ear area accounts for less than 1 / 4 of the total ear area;

[0050] Level 3: The dead ear area accounts for 1 / 4 to 1 / 2 of the whole ear area;

[0051] Level 5: The dead ear area accounts for 1 / 2 to 3 / 4 of the whole ear area;

[0052] Level 7: The area of ​​dead ears accounts for more than 3 / 4 of the total ear area.

[0053] According to the survey results, the disease index and drug efficacy were calculated according to the following formula:

[0054]

[0055]

[0056] Test results: From the results of the efficacy test on the control of wheat fusarium head blight in the field (Table 2), it can be seen that the combination of Aminopyrifen and fluopyram can significantly improve the control effect on wheat fusarium head blight. Under the same dosage, the compound agent is significantly better than the single agent. In addition, the formula of the excipients in the preparation will have a significant effect on the field control effect of the preparation. The combination of Morwet D-390, TERSPERSE 2425 and Dispersol PSR-19 can significantly improve the control effect of the suspension concentrate on wheat fusarium head blight. It is speculated that the reason may be that the combination of the above excipients is conducive to increasing the suspension rate of the active ingredients in the suspension concentrate and reducing the surface tension of the preparation.

[0057] Table 2 Field efficacy of Aminopyrifen and fluopyram combined with hydroxylamine on wheat fusarium head blight

[0058]

[0059] *Different lowercase letters after the data in the same column indicate significant differences at the P<0.05 level.

[0060] In summary, the results of indoor bioassay and field efficacy test show that the composition of the present invention has a synergistic effect, has excellent bactericidal and disease prevention effects on Fusarium fusarium and wheat fusarium fusarium caused by it, and the composition is composed of effective ingredients with different action mechanisms, which can overcome the resistance of the pathogen to a single agent, and the compound agent is safe for the test crops.

Claims

1. A fungicidal composition containing Aminopyrifen and fluopyram, characterized in that: The active ingredients are composed of Aminopyrifen and Fluopyram, and the mass ratio of Aminopyrifen and Fluopyram is (1:3)-(2:3).

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of aminopyrifen to pentopyraclostrobin is 1:3 or 2:

3.

3. The bactericidal composition according to claim 1 or 2, characterized in that: Contains agricultural auxiliary ingredients and is formulated into pesticide preparations.

4. The bactericidal composition according to claim 3, characterized in that: The total mass percentage of the active ingredients is 5%-75%.

5. The bactericidal composition according to claim 3, characterized in that: The total mass percentage of the active ingredients is 10%-60%.

6. The bactericidal composition according to claim 4, characterized in that: The pesticide formulation is a suspension, microemulsion, emulsion in water, wettable powder, water-dispersible granule or dispersible oil suspension.

7. Use of the fungicidal composition according to any one of claims 1 to 7 for preventing and controlling crop diseases.

8. The use according to claim 7, characterized in that The crop disease is wheat scab.

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