A biological cell culture sampling device

Abstract

The present invention relates to the field of biological cell culture sampling technology, and proposes a biological cell culture sampling device, including a device shell, a support frame welded and fixed on the top surface of the device shell, a servo motor and a fixed handle welded and fixed on the inner bottom surface of the support frame, the output shaft of the servo motor is rotatably connected to the bottom of the device shell and the bottom of the support frame, a first circular gear is welded and fixed on the output shaft of the servo motor, a second circular gear is meshed and connected to the first circular gear, a threaded rod is welded and fixed on the second circular gear, and the top of the threaded rod is rotatably connected to the inner top surface of the device shell. The above technical solution solves the problem that the sampling device for biological cell culture in the prior art cannot conveniently and accurately adjust the sampling volume according to the actual situation, and cannot conveniently adjust the sampling position of each sampling component according to the size of the culture dish.

Description

Technical Field

[0001] The invention relates to the technical field of biological cell culture sampling, and in particular to a biological cell culture sampling device. Background Art

[0002] Biological cell culture refers to the process of growing and reproducing animal, plant or microbial cells in vitro under artificially controlled conditions by providing appropriate nutrients, temperature, pH value and other environmental factors. Cell culture is widely used in biomedical research, drug development, genetic engineering, vaccine production and other fields. In the process of biological cell culture, in order to understand the growth status of biological cells in real time, it is necessary to use a sampling device to absorb part of the cell culture fluid for sampling to facilitate subsequent testing.

[0003] However, there are some problems with the existing sampling devices for biological cell culture. For example, a cell culture sterile sampling device with publication number CN118496973B can use multiple syringes to sample cells at different positions in the culture dish, but in the actual working process, the syringes need to be operated one by one, and multiple position conversions need to be performed to complete the sampling work at different points, which is poor in practicality and low in work efficiency; and in the actual working process, the sampling volume cannot be conveniently and accurately adjusted according to the actual situation, and the sampling position of each sampling component cannot be conveniently adjusted according to the size of the culture dish, and the applicability is poor; after the sampling device is finished working, some cell culture fluid will remain in the contact part of the sampling device with the cell culture fluid, and the existing sampling device cannot automatically remove the cell culture fluid remaining on the outer wall after the work is finished, so the cell culture fluid remaining on the outer wall of the sampling device is very likely to drip, causing environmental pollution, so it is necessary to provide a biological cell culture sampling device to meet the needs of users. Summary of the invention

[0004] The present invention proposes a biological cell culture sampling device, which solves the problem that the biological cell culture sampling device in the related art cannot conveniently and accurately adjust the sampling volume according to the actual situation, and cannot conveniently adjust the sampling position of each sampling component according to the size of the culture dish, and can automatically clean the cell culture fluid remaining on the outer wall.

[0005] The technical solution of the present invention is as follows:

[0006] A biological cell culture sampling device comprises a device shell, a support frame is welded and fixed on the top end surface of the device shell, a servo motor and a fixed handle are welded and fixed on the inner bottom end surface of the support frame, the output shaft of the servo motor is rotatably connected to the bottom of the device shell and the bottom of the support frame, a first circular gear is welded and fixed on the output shaft of the servo motor, a second circular gear is meshed and connected to the first circular gear, a threaded rod is welded and fixed on the second circular gear, the top end of the threaded rod is rotatably connected to the inner top end surface of the device shell, the bottom of the threaded rod is rotatably connected to the inner wall of the device shell, and the threaded rod is threadedly connected to the There is a connecting plate, a connecting frame is welded and fixed on the connecting plate, a quantitative adjustment sampling component is installed in the connecting frame, a positioning frame is welded and fixed on the bottom side end face of the device shell, a second return spring is welded and fixed on the side end face of the positioning frame, a second clamping rod is welded and fixed on the other end of the second return spring, the second clamping rod is slidably connected to the side end of the positioning frame, a transfer tube is slidably connected in the positioning frame, a second clamping groove is opened on the transfer tube, the end of the second clamping rod is clamped and connected in the second clamping groove, a rubber plug is clamped and connected at the end of the transfer tube, a door panel is installed on the device shell, and a through groove is opened on the top side end of the device shell.

[0007] As a preferred solution of the present invention, wherein: a first return spring is welded and fixed on the bottom side end surface of the device housing, a first clamping rod is welded and fixed on the other end of the first return spring, the first clamping rod is slidably connected to the bottom side end of the device housing, a mounting ring is slidably connected inside the device housing, a first clamping groove is opened at the side end of the mounting ring, the end of the first clamping rod is clamped and connected in the first clamping groove, a first sponge board is fixedly connected inside the mounting ring, a receiving groove is opened on the first sponge board, and a second sponge board is fixedly connected to the bottom inner wall of the receiving groove.

[0008] As a preferred solution of the present invention, wherein: the inner wall of the device shell is fitted with the outer wall of the mounting ring, the first card slot is symmetrically distributed on both sides of the mounting ring, the first card slot corresponds to the first card rod one by one, the bottom end surface of the mounting ring, the bottom end surface of the device shell, the bottom end surface of the first sponge board and the bottom end surface of the second sponge board are flush, the top end surface of the mounting ring is flush with the top end surface of the first sponge board, four storage grooves are provided, and the four storage grooves are distributed at equal angles on the first sponge board, the second sponge boards are symmetrically distributed on both sides of the storage grooves, and the second sponge boards on both sides are fitted with each other.

[0009] As a preferred solution of the present invention, wherein: the vertical center line of the first circular gear, the vertical center line of the connecting frame and the vertical center line of the device housing are located on the same vertical line, four second circular gears are provided, and the four second circular gears are distributed around the first circular gear at equal angles, the second circular gear is arranged through the through groove, the second circular gear corresponds one-to-one with the connecting plate through the threaded rod, and the second circular gear corresponds one-to-one with the through groove.

[0010] As a preferred scheme of the present invention, wherein: the quantitative adjustment sampling assembly includes a first worm and a second sleeve, the first worm being rotatably connected in the connecting frame, the first worm being meshedly connected with a first worm wheel, a rotating shaft being welded and fixed on the first worm wheel, the top end of the rotating shaft being rotatably connected to the top end surface inside the connecting frame, a turntable being welded and fixed on the bottom end of the rotating shaft, the turntable being rotatably connected to the inner wall of the connecting frame through a bearing, a first guide groove being penetrated through the turntable, a fixing plate being welded and fixed in the connecting frame, a second guide groove being penetrated through the fixing plate, the fixing plate and the rotating shaft being rotatably connected, the rotating shaft being connected to the center part of the turntable and the fixing plate, four first guide grooves and four second guide grooves being each provided, the four first guide grooves being distributed at equal angles on the turntable, the four second guide grooves being distributed at equal angles on the fixing plate, and the first guide groove being inclined.

[0011] As a preferred solution of the present invention, the second worm is rotatably connected in the connecting frame, the second worm is meshedly connected to the second worm wheel, the first sleeve is welded and fixed to the second worm wheel, the first sleeve is sleeved on the rotating shaft, the bottom end of the first sleeve is rotatably connected to the top surface of the fixed plate through a bearing, the first sleeve is welded and fixed to the first bevel gear, the first bevel gear is meshedly connected to the second bevel gear, the second bevel gear is welded and fixed to the driven shaft, and the end of the driven shaft is rotatably connected to the inner wall of the connecting frame A limiting rod is welded and fixed on the driven shaft, the second sleeve is sleeved on the driven shaft and the limiting rod, the second sleeve is slidingly connected to the driven shaft and the limiting rod, a coil is fixedly connected to the second sleeve, a traction rope is wound on the coil, a support plate is rotatably connected to the second sleeve, a guide tube is welded and fixed to the bottom end of the support plate, a guide block is welded and fixed to the guide tube, the guide block is slidingly connected in the second guide groove, the guide tube is slidingly connected in the first guide groove, and a sampling tube is welded and fixed to the bottom end of the guide tube.

[0012] As a preferred solution of the present invention, wherein: the inner diameter of the first sleeve is larger than the diameter of the rotating shaft, four second bevel gears are arranged, and the four second bevel gears are distributed on the first bevel gear at equal angles, the second bevel gears correspond one-to-one with the second sleeve through the driven shaft, the limit rods are symmetrically distributed on both sides of the driven shaft, the second sleeve corresponds one-to-one with the coil, the traction rope and the guide tube respectively, the guide tube corresponds one-to-one with the guide block and the sampling tube respectively, the guide block is rectangular, and the guide block fits with the inner wall of the second guide groove.

[0013] As a preferred scheme of the present invention, wherein: a partition is welded and fixed in the sampling tube, a first connecting spring is welded and fixed on the side end face of the partition, the other end of the first connecting spring is fixedly connected to a piston, the piston is made of rubber, the piston is slidably connected in the sampling tube, a guide wheel is installed in the sampling tube, the traction rope is slidably connected in the guide tube, the traction rope is slidably connected on the guide wheel, the traction rope is slidably connected to the partition and the sampling tube, the bottom end of the traction rope is fixedly connected to the side end face of the piston, a sampling groove is provided on the sampling tube, a telescopic sleeve rod and a second connecting spring are welded and fixed on the side end face of the sampling tube, the other end of the telescopic sleeve rod and the other end of the second connecting spring are both welded and fixed in the sealing tube, a rubber pad is fixedly connected to the inner wall of the sealing tube, and a push plate is welded and fixed on the side end face of the sealing tube.

[0014] As a preferred solution of the present invention, the piston fits with the inner wall of the sampling tube, the outer wall of the sampling tube fits with the rubber pad, the sampling grooves are symmetrically distributed on the upper and lower sides of the sampling tube, the telescopic sleeve is connected to the middle part of the side end of the sampling tube, the width of the push plate is equal to the width of the sampling tube, and the sampling tube is made of transparent material.

[0015] As a preferred solution of the present invention, there are four positioning frames, which are distributed at equal angles on the bottom outer wall of the device shell, and the positioning frames correspond to the transfer tubes one by one, and the outer wall of the transfer tube fits with the inner wall of the positioning frames.

[0016] The working principle and beneficial effects of the present invention are:

[0017] 1. The present invention is provided with a quantitative adjustment sampling assembly, which utilizes the second worm and the second worm gear to drive the first sleeve to rotate stably, and combines with each second bevel gear on the first bevel gear to drive each driven shaft to rotate synchronously, so that each traction rope can be synchronously wound and unwound through the coils on each second sleeve. Under the synchronous winding and unwinding action of each traction rope, combined with the first connecting spring, each piston can be driven to move synchronously inside the corresponding sampling tube, so that the internal storage space of each sampling tube can be adjusted at the same time, so that the sampling amount of the cell culture solution can be conveniently adjusted according to actual needs, avoiding excessive sampling amount, and leading to The balance of the cell culture system is destroyed; and each sampling tube can be used to simultaneously perform multi-point sampling at various locations inside the cell culture dish, so as to obtain cell samples at different locations, thereby increasing the accuracy of subsequent cell detection data; and the first worm and the first worm wheel can drive the turntable on the rotating shaft to rotate stably, and under the joint guiding action of the first guide groove and the second guide groove, the sampling tubes on each guide tube can be moved to the middle or the side at the same time, thereby being able to conveniently adjust the sampling position of each sampling tube according to the size of the cell culture dish, thereby effectively improving the practicability and applicability of the sampling device, and at the same time improving the working efficiency of the sampling device.

[0018] 2. The present invention is provided with a threaded rod, a first sponge plate and a second sponge plate. The second circular gears on the first circular gear can drive the corresponding threaded rods to rotate synchronously, and then the quantitative adjustment sampling assembly can be driven to move downward or upward through the connecting frame on the connecting plate. By driving the quantitative adjustment sampling assembly downward, the convenience of subsequent sampling work of the quantitative adjustment sampling assembly can be ensured; by driving the quantitative adjustment sampling assembly upward, the storage and protection work of the quantitative adjustment sampling assembly can be conveniently completed, and the convenience and stability of the subsequent carrying and transportation work of the sampling device can be ensured; and after the quantitative adjustment sampling assembly is stored, the first sponge plate and the second sponge plate are used to transfer the sample. Under the joint action, the cell culture fluid remaining on the outer wall of the bottom of the quantitative adjustment sampling component can be automatically wrapped and absorbed to realize automatic cleaning, thereby preventing the cell culture fluid from adhering to the quantitative adjustment sampling component and dripping, which leads to environmental pollution, and increases the diversity and convenience of the use of the sampling device; and under the cooperation of the first clamping rod and the first clamping groove, the first sponge plate and the second sponge plate can be conveniently installed and disassembled through the mounting ring, thereby ensuring the convenience of subsequent cleaning and replacement of the first sponge plate and the second sponge plate, thereby ensuring the stability and convenience of subsequent repeated working states of the first sponge plate and the second sponge plate.

[0019] 3. The present invention is provided with a positioning frame and a transfer tube. The sampling device is turned over as a whole, and the rubber stopper on the transfer tube is opened. Then, each sampling tube is moved to the outermost side and to the transfer tube. At this time, under the movement of each sampling tube, the sealing cylinder can be automatically moved by the push plate. At this time, the cell culture fluid sampled at each point can be automatically transported to the corresponding transfer tube, thereby ensuring the convenience and efficiency of the subsequent transportation and detection of the cell culture fluid, and effectively improving the subsequent detection efficiency of the biological cell culture fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the connection structure between the driven shaft and the limit rod of the present invention;

[0023] Figure 3 It is a schematic diagram of the connection structure between the positioning frame and the transfer pipe of the present invention;

[0024] Figure 4 It is a schematic diagram of the connection structure between the storage groove and the second sponge board of the present invention;

[0025] Figure 5 It is a schematic diagram of the main cross-sectional structure of the housing of the device of the present invention;

[0026] Figure 6 It is a schematic diagram of the main cross-sectional structure of the connection frame of the present invention;

[0027] Figure 7 It is a schematic diagram of the main cross-sectional structure of the turntable of the present invention;

[0028] Figure 8 is a schematic diagram of a top view of the structure of a first worm gear of the present invention;

[0029] Fig. 9 is a schematic diagram of a top view of the structure of the second worm gear of the present invention;

[0030] Fig.10 is a schematic diagram of a top view of the structure of a second circular gear of the present invention;

[0031] Fig.11 is a schematic diagram of a top view of the structure of the first guide groove of the present invention;

[0032] Fig.12 is a schematic diagram of a top view of the structure of the second guide groove of the present invention;

[0033] Fig.13 It is a schematic diagram of the structure of the driven shaft from a top view of the present invention;

[0034] Fig.14 It is a schematic diagram of the main cross-sectional structure of the sampling tube of the present invention;

[0035] Fig.15 It is a schematic diagram of the side cross-sectional structure of the sampling tube of the present invention;

[0036] Fig.16 It is a schematic diagram of the top-sectional structure of the housing of the device of the present invention;

[0037] Fig.17 This is a bottom view of the structure of the first sponge board of the present invention;

[0038] Fig.18 This is a schematic diagram of the main cross-sectional structure of the first sponge board of the present invention;

[0039] Fig.19 This is a schematic diagram of the main cross-sectional structure of the transfer pipe of the present invention;

[0040] Fig. 20 It is a schematic diagram of the flip structure of the housing of the device of the present invention.

[0041] In the figure: 1, device housing; 2, support frame; 3, servo motor; 4, first circular gear; 5, second circular gear; 6, threaded rod; 7, connecting plate; 8, connecting frame; 9, quantitative adjustment sampling assembly; 901, first worm; 902, first worm wheel; 903, rotating shaft; 904, turntable; 905, first guide groove; 906, fixing plate; 907, second guide groove; 908, second worm; 909, second worm wheel; 910, first sleeve; 911, first bevel gear; 912, second bevel gear; 913, driven shaft; 914, limit rod; 915, second sleeve; 916, coil; 917, traction rope; 918, support plate; 919 , guide tube; 920, guide block; 921, sampling tube; 922, partition; 923, first connecting spring; 924, piston; 925, guide wheel; 926, sampling slot; 927, telescopic sleeve; 928, second connecting spring; 929, sealing tube; 930, rubber pad; 931, push plate; 10, first return spring; 11, first clamping rod; 12, mounting ring; 13, first clamping slot; 14, first sponge plate; 15, storage slot; 16, second sponge plate; 17, positioning frame; 18, second return spring; 19, second clamping rod; 20, transfer tube; 21, second clamping slot; 22, rubber plug; 23, fixed handle; 24, door panel; 25, through slot. DETAILED DESCRIPTION

[0042] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Example 1: Figure 1 to Figure 20 As shown, this embodiment proposes a biological cell culture sampling device, including a device shell 1, a support frame 2 is welded and fixed on the top surface of the device shell 1, a servo motor 3 and a fixed handle 23 are welded and fixed on the inner bottom surface of the support frame 2, the output shaft of the servo motor 3 is rotatably connected to the bottom of the device shell 1 and the bottom of the support frame 2, a first circular gear 4 is welded and fixed on the output shaft of the servo motor 3, a second circular gear 5 is meshed and connected to the first circular gear 4, a threaded rod 6 is welded and fixed on the second circular gear 5, and the top of the threaded rod 6 is rotatably connected to On the inner top surface of the device housing 1, the bottom of the threaded rod 6 is rotatably connected to the inner wall of the device housing 1, and a connecting plate 7 is threadedly connected to the threaded rod 6, and a connecting frame 8 is welded and fixed on the connecting plate 7, and a quantitative adjustment sampling component 9 is installed in the connecting frame 8. A positioning frame 17 is welded and fixed on the bottom side end surface of the device housing 1, and a second return spring 18 is welded and fixed on the side end surface of the positioning frame 17. The other end of the second return spring 18 is welded and fixed with a second clamping rod 19, and the second clamping rod 19 penetrates and is slidably connected to the side end of the positioning frame 17, and a transfer pipe 2 is slidably connected in the positioning frame 17. 0, a second card slot 21 is provided on the transport tube 20, the end of the second card rod 19 is snap-connected in the second card slot 21, a rubber plug 22 is snap-connected at the end of the transport tube 20, a door panel 24 is installed on the device housing 1, a through slot 25 is penetrated through the top side end of the device housing 1, and the quantitative adjustment sampling component 9 can be used to conveniently adjust the sampling amount of the cell culture fluid according to actual needs to avoid excessive sampling amount, which leads to the destruction of the balance of the cell culture system; and multiple points sampling can be performed at various places inside the cell culture dish at the same time, so as to obtain cell samples at different positions, The accuracy of subsequent cell detection data is increased, and the sampling positions of each point can be conveniently adjusted according to the size of the cell culture dish, which effectively improves the practicality and applicability of the sampling device, and at the same time improves the working efficiency of the sampling device. The second circular gears 5 on the first circular gear 4 can drive the corresponding threaded rods 6 to rotate synchronously, and then the quantitative adjustment sampling component 9 can be driven to move downward or upward through the connecting frame 8 on the connecting plate 7. By driving the quantitative adjustment sampling component 9 to move downward, the convenience of subsequent sampling work of the quantitative adjustment sampling component 9 can be ensured.

[0044] Example 2: Figure 1 to Figure 20As shown, based on the same concept as the above-mentioned embodiment 1, this embodiment also proposes a biological cell culture sampling device.

[0045] In this embodiment, a first return spring 10 is welded and fixed on the bottom side end surface of the device housing 1, and a first clamping rod 11 is welded and fixed on the other end of the first return spring 10. The first clamping rod 11 penetrates and is slidably connected to the bottom side end of the device housing 1, and a mounting ring 12 is slidably connected in the device housing 1. A first clamping groove 13 is provided at the side end of the mounting ring 12, and the end of the first clamping rod 11 is clamped and connected in the first clamping groove 13. A first sponge plate 14 is fixedly connected in the mounting ring 12, and a storage groove 15 is penetrated and provided on the first sponge plate 14. A second sponge plate 16 is fixedly connected to the bottom inner wall of the storage groove 15. Under the cooperation of the first clamping rod 11 and the first clamping groove 13, the clamping installation and disassembly and separation of the first sponge plate 14 and the second sponge plate 16 can be conveniently completed through the mounting ring 12, thereby ensuring the convenience of subsequent cleaning and replacement of the first sponge plate 14 and the second sponge plate 16, thereby ensuring the stability and convenience of subsequent repeated working states of the first sponge plate 14 and the second sponge plate 16.

[0046] In this embodiment, the inner wall of the device housing 1 is fitted with the outer wall of the mounting ring 12, the first card grooves 13 are symmetrically distributed on both sides of the mounting ring 12, the first card grooves 13 correspond one by one to the first card rods 11, the bottom end surface of the mounting ring 12, the bottom end surface of the device housing 1, the bottom end surface of the first sponge plate 14 and the bottom end surface of the second sponge plate 16 are flush, the top end surface of the mounting ring 12 is flush with the top end surface of the first sponge plate 14, four receiving grooves 15 are provided, and the four receiving grooves 15 are distributed at equal angles on the first sponge plate 14, the second sponge plates 16 are symmetrically distributed on both sides of the storage grooves 15, and the second sponge plates 16 on both sides are fitted with each other. After the quantitative adjustment sampling component 9 is received, under the joint action of the first sponge plate 14 and the second sponge plate 16, the cell culture fluid remaining on the bottom outer wall of the quantitative adjustment sampling component 9 can be automatically wrapped and absorbed, and automatic cleaning is realized to avoid the cell culture fluid adhering to the quantitative adjustment sampling component 9, dripping, and environmental pollution, thereby increasing the diversity and convenience of use of the sampling device.

[0047] In this embodiment, the vertical center line of the first circular gear 4, the vertical center line of the connecting frame 8 and the vertical center line of the device housing 1 are located on the same vertical line. Four second circular gears 5 are provided, and the four second circular gears 5 are distributed at equal angles around the first circular gear 4. The second circular gears 5 are penetrated and arranged in the through groove 25. The second circular gears 5 correspond one-to-one with the connecting plate 7 through the threaded rod 6. The second circular gears 5 correspond one-to-one with the through groove 25. The second circular gears 5 on the first circular gear 4 can drive the corresponding threaded rod 6 to rotate synchronously, and then drive the quantitative adjustment sampling assembly 9 to move upward, which can conveniently complete the storage and protection work of the quantitative adjustment sampling assembly 9, and ensure the convenience and stability of the subsequent carrying and transportation of the sampling device.

[0048] In this embodiment, the quantitative adjustment sampling assembly 9 includes a first worm 901 and a second sleeve 915. The first worm 901 is rotatably connected in the connecting frame 8. The first worm 901 is meshedly connected with a first worm wheel 902. A rotating shaft 903 is welded and fixed to the first worm wheel 902. The top end of the rotating shaft 903 is rotatably connected to the top end surface inside the connecting frame 8. A rotating disk 904 is welded and fixed to the bottom end of the rotating shaft 903. The rotating disk 904 is rotatably connected to the inner wall of the connecting frame 8 through a bearing. A first guide groove 905 is penetrated on the rotating disk 904. A fixed plate 906 is welded and fixed in the connecting frame 8. A second guide groove 907 is penetrated on the fixed plate 906. The fixed plate 906 is rotatably connected to the rotating shaft 903. The rotating shaft 903 is connected to In the central part of the rotating disk 904 and the fixed plate 906, four first guide grooves 905 and four second guide grooves 907 are provided. The four first guide grooves 905 are distributed at equal angles on the rotating disk 904, and the four second guide grooves 907 are distributed at equal angles on the fixed plate 906. The first guide groove 905 is inclined. A second worm 908 is rotatably connected in the connecting frame 8. A second worm gear 909 is meshedly connected to the second worm 908. A first sleeve 910 is welded and fixed to the second worm gear 909. The first sleeve 910 is sleeved on the rotating shaft 903. The bottom end of the first sleeve 910 is rotatably connected to the top surface of the fixed plate 906 through a bearing. A first bevel gear 911 is welded and fixed to the first sleeve 910. A second bevel gear 912 is welded and fixed with a driven shaft 913, the end of the driven shaft 913 is rotatably connected to the inner wall of the connecting frame 8, a limiting rod 914 is welded and fixed to the driven shaft 913, a second sleeve 915 is sleeved on the driven shaft 913 and the limiting rod 914, the second sleeve 915 is limitedly slidably connected to the driven shaft 913 and the limiting rod 914, a coil 916 is fixedly connected to the second sleeve 915, a traction rope 917 is wound around the coil 916, a support plate 918 is rotatably connected to the second sleeve 915, a guide tube 919 is welded and fixed to the bottom end of the support plate 918, a guide block 920 is welded and fixed to the guide tube 919, the guide block 920 is limitedly slidably connected in the second guide groove 907, the guide tube 9 19 is limitedly slidably connected in the first guide groove 905, and a sampling tube 921 is welded and fixed at the bottom end of the guide tube 919. The inner diameter of the first sleeve 910 is larger than the diameter of the rotating shaft 903. Four second bevel gears 912 are provided, and the four second bevel gears 912 are distributed at equal angles on the first bevel gear 911. The second bevel gears 912 correspond to the second sleeve 915 one by one through the driven shaft 913. The limiting rods 914 are symmetrically distributed on both sides of the driven shaft 913. The second sleeve 915 corresponds to the coil 916, the traction rope 917 and the guide tube 919 one by one respectively. The guide tube 919 corresponds to the guide block 920 and the sampling tube 921 one by one respectively. The guide block 920 is rectangular, and the guide block 920 fits the inner wall of the second guide groove 907.The sampling tubes 921 can be used to simultaneously perform multi-point sampling at various locations inside the cell culture dish, so as to obtain cell samples at different locations and increase the accuracy of subsequent cell detection data; and the first worm 901 and the first worm wheel 902 can drive the turntable 904 on the rotating shaft 903 to rotate stably, and under the joint guidance of the first guide groove 905 and the second guide groove 907, the sampling tubes 921 on the guide tubes 919 can be moved to the middle or the side at the same time, so that the sampling position of each sampling tube 921 can be conveniently adjusted according to the size of the cell culture dish.

[0049] In this embodiment, a partition 922 is welded and fixed in the sampling tube 921, a first connecting spring 923 is welded and fixed on the side end surface of the partition 922, the other end of the first connecting spring 923 is fixedly connected to a piston 924, the piston 924 is made of rubber, the piston 924 is slidably connected in the sampling tube 921, a guide wheel 925 is installed in the sampling tube 921, the traction rope 917 is slidably connected in the guide tube 919, the traction rope 917 is slidably connected to the guide wheel 925, the traction rope 917 is connected to the partition 922 and the sampling tube 921 are all through sliding connection, the bottom end of the traction rope 917 is fixedly connected to the side end surface of the piston 924, a sampling groove 926 is provided on the sampling tube 921, a telescopic sleeve 927 and a second connecting spring 928 are welded and fixed to the side end surface of the sampling tube 921, the other end of the telescopic sleeve 927 and the other end of the second connecting spring 928 are welded and fixed in the sealing cylinder 929, a rubber pad 930 is fixedly connected to the inner wall of the sealing cylinder 929, a push plate 931 is welded and fixed to the side end surface of the sealing cylinder 929, and the piston 9 24 is fitted with the inner wall of the sampling tube 921, the outer wall of the sampling tube 921 is fitted with the rubber pad 930, the sampling grooves 926 are symmetrically distributed on the upper and lower sides of the sampling tube 921, the telescopic sleeve 927 is connected to the middle part of the side end of the sampling tube 921, the width of the push plate 931 is equal to the width of the sampling tube 921, the sampling tube 921 is made of transparent material, the second worm 908 and the second worm gear 909 can drive the first sleeve 910 to rotate stably, and the second bevel gear 911 on each second bevel gear 911 can be combined with the first bevel gear 911 to rotate stably. 12 can drive each driven shaft 913 to rotate synchronously, and then each traction rope 917 can be synchronously wound and unwound through the coil 916 on each second sleeve 915. Under the synchronous winding and unwinding action of each traction rope 917, combined with the first connecting spring 923, each piston 924 can be driven to move synchronously inside the corresponding sampling tube 921, and then the internal storage space of each sampling tube 921 can be adjusted at the same time, so that the sampling amount of the cell culture fluid can be conveniently adjusted according to actual needs.

[0050] In this embodiment, four positioning frames 17 are provided, and the four positioning frames 17 are distributed at equal angles on the bottom outer wall of the device housing 1. The positioning frames 17 correspond to the transfer tubes 20 one by one, and the outer wall of the transfer tube 20 fits with the inner wall of the positioning frames 17. By turning the sampling device over as a whole and opening the rubber stopper 22 on the transfer tube 20, each sampling tube 921 is then moved to the outermost side and to the transfer tube 20. At this time, under the movement of each sampling tube 921, the sealing cylinder 929 can be automatically moved by the push plate 931. At this time, the cell culture fluid sampled at each point can be automatically transported to the corresponding transfer tube 20, thereby ensuring the convenience and efficiency of subsequent transportation and detection of the cell culture fluid.

[0051] It should be noted that the present invention is a biological cell culture sampling device. First, the staff can drive the servo motor 3 on the support frame 2. Under the driving action of the servo motor 3, the first circular gear 4 can be driven to rotate stably through the output shaft. At this time, the first circular gear 4 can drive the corresponding threaded rod 6 to rotate simultaneously through each meshing second circular gear 5. Under the simultaneous rotation of each threaded rod 6, the connecting frame 8 can be driven to move downward through the threaded connecting plate 7. Under the downward movement of the connecting frame 8, the sampling tube 921 can be pushed downward through the guide tube 919. Under the downward movement of the sampling tube 921, the second sponge plates 16 on both sides of the bottom can be opened. At this time, each sampling tube 921 can pass through the storage groove 15 on the first sponge plate 14 and move downward until the connecting plate 7 moves to the lowest end of the threaded rod 6. At this time, each sampling tube 921 and the push plate 931 on the corresponding sealing cylinder 929 can be separated from the storage groove 15 on the first sponge plate 14.

[0052] Then, the staff can open the door panel 24 on the device housing 1 and rotate the first worm 901 on the connecting frame 8. Under the rotation of the first worm 901, the first worm wheel 902 connected in meshing can drive the turntable 904 on the rotating shaft 903 to rotate stably. Under the stable rotation of the turntable 904, the guide tube 919 can be moved stably along the second guide groove 907 on the fixed plate 906 through the second guide groove 907. Under the guiding and limiting action of the guide block 920 and the second guide groove 907, the forward and reverse rotation of the first worm 901 can drive each guide tube 919 to move to the side or the middle at the same time. Under the synchronous movement of each guide tube 919, the corresponding sampling tube 921 at the bottom can be driven to move synchronously, so that the sampling position of each sampling tube 921 can be conveniently adjusted according to the size of the cell culture dish.

[0053] During the movement of each guide tube 919, the second sleeve 915 can be driven to slide stably on the driven shaft 913 and the limit rod 914 through the top support plate 918. Then the staff can rotate the second worm 908 on the connecting frame 8. Under the rotation of the second worm 908, the meshing second worm gear 909 can drive the first sleeve 910 to rotate stably. Under the rotation of the first sleeve 910, the first bevel gear 911 can be driven to rotate, and then the corresponding driven shaft 913 can be driven to rotate synchronously through each meshing second bevel gear 912. At this time, under the rotation of each driven shaft 913, combined with the limit rod 914, the second sleeve 915 can be driven to rotate stably. At this time, under the rotation of each second sleeve 915, the coil 916 can synchronously reel in or unreel the traction ropes 917 in each guide tube 919; by reeling in the traction rope 917, the piston 924 can be pulled through the guide wheel 925 to move in the sampling tube 921 toward the partition 922. Similarly, by unreeling the traction rope 917, the piston 924 can be pushed in the sampling tube 921 toward the direction away from the partition 922 under the elastic action of the first connecting spring 923 on the partition 922; by driving each piston 924 to synchronously move inside the corresponding sampling tube 921, the internal storage space of each sampling tube 921 can be adjusted at the same time, so that the sampling amount of the cell culture fluid can be conveniently adjusted according to actual needs, so as to avoid excessive sampling amount, which may cause the balance of the cell culture system to be destroyed;

[0054] After the sampling position and sampling volume of each sampling tube 921 are adjusted, the staff can hold the fixed handle 23 on the support frame 2, and insert each sampling tube 921 into the biological cell culture dish through the whole picking device, and immerse each sampling tube 921 in the biological cell culture fluid. Then the staff only needs to turn the push plate 931, and by turning the push plate 931, the sealing cylinder 929 can be driven to move away from the sampling tube 921. At this time, the biological cell culture fluid in the biological cell culture dish can automatically enter the sampling groove 926. The sampling tube 921 is filled with the adjusted material storage space in the sampling tube 921, and then the staff can release the push plate 931. At this time, under the elastic action of the second connecting spring 928, combined with the guidance of the telescopic sleeve 927, the sealing cylinder 929 can be driven to automatically move to the sampling tube 921, and combined with the rubber pad 930, the sampling slot 926 on the sampling tube 921 can be automatically sealed. Then the staff can lift the device as a whole, so that each sampling tube 921 moves out of the biological cell culture dish, and the sampling work is completed;

[0055] Then the staff can rotate the first worm 901, and the meshing first worm wheel 902 can drive the turntable 904 on the rotating shaft 903 to rotate stably. Under the joint guiding action of the first guide groove 905 and the second guide groove 907, each guide tube 919 can be moved to the middle at the same time until each guide tube 919 moves to the middle. Then the servo motor 3 on the support frame 2 can be driven. Under the driving action of the servo motor 3, the first circular gear 4 can be driven to rotate in the opposite direction through the output shaft. At this time, the first circular gear 4 can drive the corresponding threaded rod 6 to rotate in the opposite direction at the same time through each meshing second circular gear 5. Under the reverse rotation action of each threaded rod 6, the threaded connecting plate 7 can be driven The movable connecting frame 8 moves upward to reset. Through the upward reset of the connecting frame 8, the sampling tube 921 can be driven to move upward through the guide tube 919. At this time, each sampling tube 921 can stably move into the storage groove 15 on the first sponge plate 14, which can conveniently complete the storage and protection work of the quantitative adjustment sampling component 9, and ensure the convenience and stability of the subsequent carrying and transportation of the sampling device; and after the quantitative adjustment sampling component 9 is stored, under the joint action of the first sponge plate 14 and the second sponge plate 16, the cell culture fluid remaining on the outer wall of the bottom of the quantitative adjustment sampling component 9 can be automatically wrapped and absorbed, and the automatic cleaning work is realized, so as to avoid the cell culture fluid adhering to the quantitative adjustment sampling component 9, dripping, and causing environmental pollution;

[0056] Then the staff can carry the whole device to the detection equipment, then the staff can drive the servo motor 3 again, and then drive each sampling tube 921 to move to the outside of the device housing 1, then the staff can flip the whole device 180°, at this time the support frame 2 is directly below, and the support frame 2 on the device housing 1 is placed on the workbench, then the staff can open the rubber plug 22 on each transfer tube 20, after the rubber plug 22 is opened, the staff can rotate the first worm 901 to push the sampling tube 921 on each guide tube 919 1 simultaneously moves to the side until it moves to the most side, and then the servo motor 3 can be driven again to drive the sampling tubes 921 on each guide tube 919 to move toward the device housing 1 through the connecting frame 8 and fit with the device housing 1, and then the first worm 901 can be rotated continuously. At this time, under the reverse rotation of the first worm 901, each sampling tube 921 can be driven to move to the middle at the same time. At this time, during the movement of each sampling tube 921, the transport tube 20 will block the push plate 931. In other words, during the movement of the sampling tube 921, the transport tube 20 can automatically push open the sealing cylinder 929 through the push plate 931, and at this time, the cell culture fluid in each sampling tube 921 can be automatically transported downward to the corresponding transport tube 20 through the sampling slot 926; then the staff can store the quantitative adjustment sampling assembly 9 into the device housing 1 again through the same operation as above;

[0057] Then the staff can engage the rubber stopper 22 on the transfer tube 20 to achieve the sealing work of the transfer tube 20, and pull the second clamping rod 19 on the positioning frame 17 outwards until the second clamping rod 19 moves out of the second clamping groove 21 on the transfer tube 20, so as to conveniently complete the disassembly and separation of the transfer tube 20, thereby ensuring the convenience of subsequent taking of the transfer tube 20. By using the transfer tube 20, the convenience and efficiency of subsequent transportation and detection of the cell culture fluid can be ensured. After the transfer tube 20 is used and cleaned, the staff can pull the second clamping rod 19 on the positioning frame 17 outwards in advance and insert the transfer tube 20 into the positioning frame 17. Then the staff can loosen the second clamping rod 19. At this time, under the elastic action of the second reset spring 18, the second clamping rod 19 can be driven to automatically engage with the second clamping groove 21 on the transfer tube 20, completing the clamping and fixing of the transfer tube 20, and ensuring the stability of the subsequent working state of the transfer tube 20;

[0058] When the sampling device finishes working, the staff can complete the disassembly of the mounting ring 12 by pulling the first clamping rod 11 on the device housing 1 outward until the first clamping rod 11 moves out of the first clamping groove 13 on the mounting ring 12, and then the first sponge plate 14 and the second sponge plate 16 can be cleaned and dried, and the staff can insert the cleaned and dried mounting ring 12 into the device housing 1, and the staff needs to pull the first clamping rod 11 on the device housing 1 outward in advance. After the mounting ring 12 is inserted to the appropriate position, the first clamping rod 11 on the device housing 1 can be released. At this time, under the elastic action of the first return spring 10, the first clamping rod 11 can be driven to automatically engage with the first clamping groove 13 on the mounting ring 12, completing the clamping installation of the mounting ring 12 and ensuring the overall stability of the subsequent work of the mounting ring 12.

[0059] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A biological cell culture sampling device, characterized in that: The device comprises a housing (1), a support frame (2) is welded and fixed on the top end surface of the housing (1), a servo motor (3) and a fixed handle (23) are welded and fixed on the inner bottom end surface of the support frame (2), an output shaft of the servo motor (3) is rotatably connected to the bottom of the housing (1) and the bottom of the support frame (2), a first circular gear (4) is welded and fixed on the output shaft of the servo motor (3), a second circular gear (5) is meshingly connected to the first circular gear (4), a threaded rod (6) is welded and fixed to the second circular gear (5), the top end of the threaded rod (6) is rotatably connected to the inner top end surface of the housing (1), and the bottom of the threaded rod (6) is rotatably connected to the housing (1). On the inner wall of the device housing (1), a connecting plate (7) is threadedly connected to the threaded rod (6), a connecting frame (8) is welded and fixed to the connecting plate (7), a quantitative adjustment sampling assembly (9) is installed in the connecting frame (8), a positioning frame (17) is welded and fixed to the bottom side end surface of the device housing (1), a second return spring (18) is welded and fixed to the side end surface of the positioning frame (17), a second clamping rod (19) is welded and fixed to the other end of the second return spring (18), the second clamping rod (19) penetrates and is slidably connected to the side end of the positioning frame (17), a transfer pipe (20) is slidably connected in the positioning frame (17), a second clamping groove (21) is provided on the transfer pipe (20), and the end of the second clamping rod (19) is clamped and connected to the In the second slot (21), the end of the transfer tube (20) is snap-connected with a rubber plug (22), a door panel (24) is installed on the device housing (1), and a through slot (25) is penetrated through the top side end of the device housing (1), and the quantitative adjustment sampling assembly (9) comprises a first worm (901) and a second sleeve (915), the first worm (901) is rotatably connected in the connection frame (8), the first worm (901) is meshingly connected with a first worm wheel (902), a rotating shaft (903) is welded and fixed to the first worm wheel (902), the top end of the rotating shaft (903) is rotatably connected to the top end surface inside the connection frame (8), and the bottom end of the rotating shaft (903) is welded and fixed to a rotating disk (904), The rotating disk (904) is rotatably connected to the inner wall of the connecting frame (8) via a bearing, a first guide groove (905) is formed through the rotating disk (904), a fixing plate (906) is welded and fixed in the connecting frame (8), a second guide groove (907) is formed through the fixing plate (906), the fixing plate (906) is rotatably connected to the rotating shaft (903), the rotating shaft (903) is connected to the center of the rotating disk (904) and the fixing plate (906), four first guide grooves (905) and four second guide grooves (907) are each provided, the four first guide grooves (905) are distributed at equal angles on the rotating disk (904), and the four second guide grooves (907) are distributed at equal angles on the fixing plate (906).The first guide groove (905) is inclined, a second worm (908) is rotatably connected in the connection frame (8), a second worm wheel (909) is meshedly connected to the second worm (908), a first sleeve (910) is welded and fixed to the second worm wheel (909), the first sleeve (910) is sleeved on the rotating shaft (903), the bottom end of the first sleeve (910) is rotatably connected to the top surface of the fixing plate (906) through a bearing, a first bevel gear (911) is welded and fixed to the first sleeve (910), a second bevel gear (912) is meshedly connected to the first bevel gear (911), a driven shaft (913) is welded and fixed to the second bevel gear (912), the end of the driven shaft (913) is rotatably connected to the inner wall of the connection frame (8), and the driven shaft (913) is welded and fixed to the inner wall of the connection frame (8). A limit rod (914) is provided, the second sleeve (915) is sleeved on the driven shaft (913) and the limit rod (914), the second sleeve (915) is slidably connected to the driven shaft (913) and the limit rod (914), the second sleeve (915) is fixedly connected to a coil (916), a traction rope (917) is wound around the coil (916), a support plate (918) is rotatably connected to the second sleeve (915), a guide tube (919) is welded and fixed to the bottom end of the support plate (918), a guide block (920) is welded and fixed to the guide tube (919), the guide block (920) is slidably connected to the second guide groove (907), the guide tube (919) is slidably connected to the first guide groove (905), and a sampling tube (921) is welded and fixed to the bottom end of the guide tube (919).

2. A biological cell culture sampling device according to claim 1, characterized in that: A first return spring (10) is welded and fixed on the side end surface of the bottom of the device housing (1), and a first clamping rod (11) is welded and fixed on the other end of the first return spring (10), and the first clamping rod (11) is slidably connected to the bottom side end of the device housing (1), and a mounting ring (12) is slidably connected inside the device housing (1), and a first clamping groove (13) is opened at the side end of the mounting ring (12), and the end of the first clamping rod (11) is clamped and connected in the first clamping groove (13), and a first sponge plate (14) is fixedly connected inside the mounting ring (12), and a receiving groove (15) is opened on the first sponge plate (14), and a second sponge plate (16) is fixedly connected to the bottom inner wall of the receiving groove (15).

3. A biological cell culture sampling device according to claim 2, characterized in that: The inner wall of the device housing (1) is fitted with the outer wall of the mounting ring (12); the first clamping grooves (13) are symmetrically distributed on both sides of the mounting ring (12); the first clamping grooves (13) correspond one-to-one with the first clamping rod (11); the bottom end surface of the mounting ring (12), the bottom end surface of the device housing (1), the bottom end surface of the first sponge plate (14) and the bottom end surface of the second sponge plate (16) are flush; the top end surface of the mounting ring (12) is flush with the top end surface of the first sponge plate (14); four receiving grooves (15) are provided, and the four receiving grooves (15) are distributed at equal angles on the first sponge plate (14); the second sponge plates (16) are symmetrically distributed on both sides of the receiving grooves (15); and the second sponge plates (16) on both sides are fitted with each other.

4. A biological cell culture sampling device according to claim 1, characterized in that: The vertical center line of the first circular gear (4), the vertical center line of the connecting frame (8) and the vertical center line of the device housing (1) are located on the same vertical line. Four second circular gears (5) are provided. The four second circular gears (5) are distributed around the first circular gear (4) at equal angles. The second circular gears (5) are arranged through the through groove (25). The second circular gears (5) correspond one-to-one with the connecting plate (7) through the threaded rod (6). The second circular gears (5) correspond one-to-one with the through groove (25).

5. A biological cell culture sampling device according to claim 1, characterized in that: The inner diameter of the first sleeve (910) is larger than the diameter of the rotating shaft (903); four second bevel gears (912) are provided, and the four second bevel gears (912) are distributed at equal angles on the first bevel gear (911); the second bevel gears (912) correspond one-to-one with the second sleeve (915) through the driven shaft (913); the limit rods (914) are symmetrically distributed on both sides of the driven shaft (913); the second sleeve (915) corresponds one-to-one with the coil (916), the traction rope (917) and the guide tube (919); the guide tube (919) corresponds one-to-one with the guide block (920) and the sampling tube (921); the guide block (920) is rectangular, and the guide block (920) fits the inner wall of the second guide groove (907).

6. A biological cell culture sampling device according to claim 5, characterized in that: A partition (922) is welded and fixed in the sampling tube (921), a first connecting spring (923) is welded and fixed on the side end surface of the partition (922), the other end of the first connecting spring (923) is fixedly connected to a piston (924), the piston (924) is made of rubber, the piston (924) is slidably connected in the sampling tube (921), a guide wheel (925) is installed in the sampling tube (921), the traction rope (917) is slidably connected in the guide tube (919), the traction rope (917) is slidably connected to the guide wheel (925), the traction rope (917) is slidably connected to the partition (922) and The sampling tubes (921) are all connected by sliding through, the bottom end of the traction rope (917) is fixedly connected to the side end surface of the piston (924), a sampling groove (926) is opened on the sampling tube (921), a telescopic sleeve rod (927) and a second connecting spring (928) are welded and fixed on the side end surface of the sampling tube (921), the other end of the telescopic sleeve rod (927) and the other end of the second connecting spring (928) are welded and fixed in the sealing tube (929), a rubber pad (930) is fixedly connected to the inner wall of the sealing tube (929), and a push plate (931) is welded and fixed on the side end surface of the sealing tube (929).

7. A biological cell culture sampling device according to claim 6, characterized in that: The piston (924) fits with the inner wall of the sampling tube (921), the outer wall of the sampling tube (921) fits with the rubber pad (930), the sampling grooves (926) are symmetrically distributed on the upper and lower sides of the sampling tube (921), the telescopic sleeve rod (927) is connected to the middle part of the side end of the sampling tube (921), the width of the push plate (931) is equal to the width of the sampling tube (921), and the sampling tube (921) is made of a transparent material.

8. The biological cell culture sampling device according to claim 1, characterized in that: Four positioning frames (17) are provided, and the four positioning frames (17) are distributed at equal angles on the bottom outer wall of the device housing (1). The positioning frames (17) correspond to the transfer tubes (20) one by one, and the outer wall of the transfer tube (20) fits the inner wall of the positioning frame (17).

Images