Microscope positioning device and method

Abstract

The invention discloses a microscope positioning device and method, and the device comprises a microscope unit and a positioning spotlight, and is characterized in that the microscope unit comprises an eyepiece, an objective lens, an objective table, a coarse focusing screw, and a reflector; the eyepiece is located at the top of the microscope unit, the objective lens is located below the eyepiece, the objective table is located below the objective lens, and the reflector is located below the objective table; a glass slide and a sheet pressing clamp are arranged on the objective table; the positioning spotlight is placed in a lens cone provided with the eyepiece, the coarse focusing screw is used for controlling the lifting of the lens cone, the objective lens and the positioning spotlight, and light generated by the positioning spotlight is emitted towards the objective lens and can be focused on a glass slide of the objective table to form a bright focus after penetrating through the objective lens; the positioning spotlight comprises a lamp bead, a storage battery and a switch; the storage battery is connected with the switch and the lamp beads through wires. The switch is connected in series with the lamp bead; according to the invention, a bright positioning point can be generated on the glass slide and is located at the central point of the light through hole, so that a precise reference point is provided for rapidly moving a sample on the glass slide to the center of the light through hole, and the problem of moving the glass slide is solved; according to the invention, blind focusing of a user can be avoided, and the problem of focal length adjustment is solved.

Description

Technical Field

[0001] The invention relates to the technical field of microscopes, and in particular to a microscope positioning device and a positioning method. Background Art

[0002] The microscope is a precision optical instrument with a history of more than 300 years. Since the invention of the microscope, people have seen many tiny organisms and the basic units that make up organisms that were invisible in the past. Some experimental observations need to be completed through the microscope, and before observation, the object needs to be positioned to enable accurate observation and imaging.

[0003] The current optical microscope has an overly large light aperture of 20 mm, making it difficult to accurately move the sample on the slide to the center of the light aperture. When placing the slide on the stage, the eyes are looking sideways, which can easily cause illusions and further increase the difficulty of moving the sample to the center of the light aperture. It is difficult to adjust the focal length, especially when the sample is not facing the light aperture, which is even more difficult without a sample for reference. It is impossible to quickly and directly observe the specified position (area) of the sample. It is impossible to directly and accurately locate the target in the field of view in the sample, which affects the observation efficiency. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a microscope positioning device and a positioning method, which can effectively solve the problems raised in the above background technology.

[0005] In order to solve the above problems, the technical solution adopted by the present invention is: a microscope positioning device, including a microscope unit and a positioning spotlight, characterized in that the microscope unit includes an eyepiece, an objective lens, a stage, a coarse focusing screw and a reflector; the eyepiece is located at the top of the microscope unit, the objective lens is located below the eyepiece, the stage is located below the objective lens, and the reflector is located below the stage; a glass slide and a slide clamp are provided on the stage; the positioning spotlight is placed in a lens barrel on which the eyepiece is installed, and the coarse focusing screw is used to control the lifting and lowering of the lens barrel, the objective lens and the positioning spotlight, and the light generated by the positioning spotlight is emitted toward the objective lens, and after passing through the objective lens, it can be focused on the glass slide on the stage to form a bright focus.

[0006] As a further preferred embodiment of the present invention, the positioning spotlight comprises a lamp bead, a battery and a switch; the battery is connected to the switch and the lamp bead respectively through a wire; the switch and the lamp bead are connected in series.

[0007] As a further preferred embodiment of the present invention, the lamp beads are LED lamps.

[0008] As a further preferred embodiment of the present invention, the positioning method comprises the following steps: S1. Adjust the reflector to make the field of vision brighter; S2. Place the slide on the stage and clamp the slide with a slide clamp; S3, turn the coarse focusing knob 1-4 to raise the lens barrel, remove the eyepiece, replace it with a positioning spotlight and put it into the lens barrel for installing the eyepiece, turn on the power switch of the positioning device, turn the coarse focusing knob to lower the lens barrel, and observe that the light spot positioning point on the slide gradually shrinks. When the positioning point becomes the smallest, stop turning the coarse focusing knob; S4. Move the slide to move the sample to the positioning point, turn off the power switch of the positioning spotlight, take out the positioning spotlight, replace it with the eyepiece, and adjust the focal length slightly to see the object image clearly.

[0009] Compared with the prior art, the present invention provides a microscope positioning device and positioning method, which have the following beneficial effects: The present invention can generate a bright positioning point located at the center of the light hole, providing an accurate reference point for quickly moving the sample on the slide to the center of the light hole, thereby solving the problem of moving the slide. By adjusting the coarse focusing screw, when the positioning point generated by the positioning spotlight becomes the smallest, the focal length has been preliminarily adjusted, which can avoid the user from blindly adjusting the focus and solve the problem of adjusting the focal length; The positioning points generated by the positioning spotlight can quickly and accurately observe the designated position of the sample, solving the observation problem of the designated position area of ​​the sample; After moving the specific target in the field of view to the center of the field of view, the positioning point generated by the positioning spotlight can be used to quickly and accurately determine the specific position of the specific target in the field of view in the sample, thereby solving the problem of the specific position of the target in the field of view in the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the disassembly of the structure of the present invention; Figure 3 This is a schematic diagram of the internal structure of the positioning spotlight; Figure 4 This is the imaging effect of the onion experiment; Figure 5 This is the imaging effect of the petal experiment; Figure 6 This is the imaging effect of the petal experiment; Figure 7 This is the imaging effect of the petal experiment; Figure 8 This is the imaging effect of the petal experiment; Fig. 9 This is the imaging effect of the petal experiment; Fig.10 This is the imaging effect of the petal experiment.

[0011] Among them: 1. Microscope unit; 1-1. Eyepiece; 1-2. Objective lens; 1-3. Stage; 1-4. Coarse focusing screw; 1-5. Reflector; 2. Positioning spotlight; 2-1. Lamp beads; 2-2. Battery; 2-3. Switch. DETAILED DESCRIPTION

[0012] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0013] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features.

[0014] In addition, if "and / or" or "and / or" appears in the full text, its meaning includes three parallel options. Taking "A and / or B" as an example, it includes option A, or option B, or a option in which both A and B are satisfied.

[0015] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0016] Reference Figure 1-3 The present invention provides a microscope positioning device, comprising a microscope unit 1 and a positioning spotlight 2, characterized in that the microscope unit 1 comprises an eyepiece 1-1, an objective lens 1-2, a stage 1-3, a coarse focusing screw 1-4 and a reflector 1-5; the eyepiece 1-1 is located at the top of the microscope unit 1, the objective lens 1-2 is located below the eyepiece 1-1, the stage 1-3 is located below the objective lens 1-2, and the reflector 1-5 is located below the stage 1-3; a glass slide and a pressing clamp are provided on the stage 1-3; the positioning spotlight 2 is placed in a lens barrel on which the eyepiece 1-1 is installed, the coarse focusing screw 1-4 is used to control the lifting and lowering of the lens barrel and the positioning spotlight 2, and the light beam of the positioning spotlight 2 is emitted from the eyepiece 1-1 and passes through the objective lens 1-2 and then is focused and displayed on the glass slide of the stage 1-3; When the positioning spotlight 2 emits strong light in the lens barrel where the eyepiece 1-1 is installed and passes through the objective lens 1-2 in the reverse direction, it will focus directly below the objective lens 1-2 to form a bright and small focus (positioning point) for accurate positioning of the specimen on the slide. The distance between the focus and the objective lens 1-2 is exactly the focal length of the objective lens, and at the same time, a relatively accurate focusing of the microscope is achieved.

[0017] As a further preferred embodiment of the present invention, the positioning spotlight 2 comprises a lamp bead 2-1, a battery 2-2 and a switch 2-3; the battery 2-2 is connected to the switch 2-3 and the lamp bead 2-1 respectively through wires; the switch 2-3 and the lamp bead 2-1 are connected in series.

[0018] As a further preferred embodiment of the present invention, the lamp bead 2-1 is an LED lamp. Experiments have shown that if the lamp bead 2-1 is a laser spotlight, the light of the laser spotlight is relatively straight and can become a light spot without focusing after being placed in it. As a result, the observer needs to readjust the focal length during use, which is prone to errors.

[0019] As a further preferred embodiment of the present invention, the positioning method comprises the following steps: S1, adjust the reflectors 1-5 to make the field of view white and bright; S2, place the slide on stage 1-3 and clamp the slide with a slide clamp; S3, turn the coarse focusing screw 1-4, raise the lens barrel, remove the eyepiece 1-1, replace it with the positioning spotlight 2 and put it into the lens barrel for installing the eyepiece 1-1, turn on the power switch 2-3 of the positioning device, turn the coarse focusing screw 1-4 to lower the lens barrel, observe that the light spot positioning point on the slide gradually shrinks, and stop turning the coarse focusing screw 1-4 when the positioning point becomes the smallest; S4. Move the slide to move the sample to the location of the positioning point, turn off the power switch 2-3 of the positioning spotlight 2, take out the positioning spotlight 2, replace it with the eyepiece 1-1, and adjust the focal length slightly to see the object image clearly.

[0020] As a specific embodiment of the present invention: 1. Observe the designated position of the sample: Take the petals of the red building flower as the experimental material and observe the fork of the flower. Figure 4-5 ; 2. Determine the specific location of the target in the field of view in the sample: Using the petals of red house flowers as experimental materials, determine the specific location of the flower glands on the petals, such as Figure 6-7 ; 3. Observe the designated location of the sample: Take the outer epidermis of the onion scale leaf as the experimental material and observe the micrograph of the "small hole" in the sample, such as Figure 8-10 .

[0021] 4. Student practical results: In order to verify the advantages of the positioning spotlight in the microscope experiment operation, some high school students were randomly selected to conduct microscope experiments. The time they used from the beginning to the observation of the object image on the same permanent mount using different methods (conventional method and method using the positioning device) was counted. The experimental results are as follows: personnel Normal time (unit: seconds) Positioning device time (unit: seconds) 1 74 30 2 56 36 3 100 28 4 54 22 5 55 32 6 78 31 7 147 45 8 31 42 average 74.38 33.25 It can be seen that when designing the microscope positioning device, reverse thinking is adopted. By using light to pass through the objective lens in reverse, a focus can be formed on the slide, which provides an important reference for positioning and focusing. The positioning device is not only novel in design, scientific in principle, and unique in conception, but also low in cost and easy to promote. More importantly, it effectively improves the defects that have existed in the microscope for more than 300 years since its birth. In practical applications, it can save students a lot of time and energy, significantly improve the efficiency of microscope experimental teaching, and greatly enhance students' learning interest and sense of accomplishment.

[0022] As needed, the above-mentioned installation, setting, provision or connection methods include but are not limited to installation, setting or connection by screws, riveting, welding or socketing, fixing, etc., and the installation, setting or connection method is selected according to the needs of the working scenario.

[0023] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A microscope positioning device, comprising a microscope unit (1) and a positioning spotlight (2), characterized in that: The microscope unit (1) comprises an eyepiece (1-1), an objective lens (1-2), a stage (1-3), a coarse focusing screw (1-4) and a reflector (1-5); the eyepiece (1-1) is located at the top of the microscope unit (1), the objective lens (1-2) is located below the eyepiece (1-1), the stage (1-3) is located below the objective lens (1-2), and the reflector (1-5) is located below the stage (1-3); a glass slide and a slide clamp are provided on the stage (1-3); the positioning spotlight (2) is placed in a lens barrel on which the eyepiece (1-1) is installed, and the coarse focusing screw (1-4) is used to control the lifting and lowering of the lens barrel, the objective lens (1-2) and the positioning spotlight (2); the light generated by the positioning spotlight (2) is emitted in the direction of the objective lens (1-2), and after passing through the objective lens (1-2), it can be focused on the glass slide of the stage (1-3) to form a bright focus.

2. The microscope positioning device according to claim 1, characterized in that: The positioning spotlight (2) comprises a lamp bead (2-1), a storage battery (2-2) and a switch (2-3); the storage battery (2-2) is connected to the switch (2-3) and the lamp bead (2-1) respectively through a wire; and the switch (2-3) and the lamp bead (2-1) are connected in series.

3. The microscope positioning device according to claim 2, characterized in that: The lamp bead (2-1) is an LED lamp.

4. A positioning method for a microscope positioning device as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Adjust the reflector (1-5) to make the field of vision brighter; S2. Place the slide on the stage (1-3) and clamp the slide with a slide clamp; S3, turn the coarse focusing knob (1-4), raise the lens barrel, remove the eyepiece (1-1), replace it with the positioning spotlight (2) and put it into the lens barrel for installing the eyepiece, turn on the power switch of the positioning device (2-3), turn the coarse focusing knob (1-4) to lower the lens barrel, observe that the light spot positioning point on the slide gradually shrinks, and stop turning the coarse focusing knob (1-4) when the positioning point becomes the smallest; S4. Move the slide to move the sample to the location of the positioning point, turn off the power switch (2-3) of the positioning spotlight (2), take out the positioning spotlight (2) and replace it with the eyepiece (1-1), and adjust the focus slightly to see the object image clearly.

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