A method for wild cultivation of dictyophora rubrovolvata by using dry bamboo resources

Abstract

This invention discloses a method for simulating the wild cultivation of *Dictyophora indicum* using dead bamboo resources, comprising the following steps: digging trenches downwards in the soil layer between *Phyllostachys edulis* groves; placing a water collection layer at the bottom of the trench; laying a lower filling layer composed of chopped bamboo residue on top of the water collection layer; laying a middle filling layer composed of a mixture of chopped bamboo residue and humus on top of the lower filling layer; constructing a cultivation trough along the length of the trench on the middle filling layer for *Dictyophora indicum* cultivation; laying a layer of pre-prepared culture medium in the cultivation trough, then placing *Dictyophora indicum* spawn with multiple holes punched around its perimeter in the middle of the culture medium, and then evenly scattering the mycelium from inside the spawn evenly on the surface of the culture medium; covering the middle filling layer and culture medium with an upper filling layer composed of sandy loam; and finally covering the upper filling layer with a covering layer. This invention has high resource utilization, significant economic benefits, and obvious ecological benefits, achieving long-term moisture retention and nutrient supplementation, and enabling fruiting for many years.

Description

Technical Field

[0001] This invention relates to the field of edible fungus cultivation technology, specifically to a method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources. Background Technology

[0002] Red-topped bamboo fungus, an important species in the development of the edible fungus industry in Guizhou Province, is characterized by high yield, rich nutritional value, and crisp and tender texture. Further research indicates that wild resources of red-topped bamboo fungus are distributed in various bamboo forests, with bamboo providing nutrients for its growth and development, especially decomposed bamboo which accelerates the growth and development of the fungus. Traditional cultivation of red-topped bamboo fungus often relies on broadleaf tree sawdust or other non-renewable resources, typically involving direct soil covering during planting. Tongzi County, a major producer of square bamboo in Guizhou Province, possesses a large-scale native square bamboo forest resource, with the current planting area exceeding 1 million mu. To increase bamboo shoot yield in square bamboo forests, thinning of older bamboo is necessary, resulting in low reuse rates and significant resource waste. Therefore, it is necessary to rationally utilize the resources of square bamboo forests to plant red-topped bamboo fungus without affecting bamboo shoot production, forming a "three-bamboo industry" of bamboo fungus, bamboo, and bamboo shoots, and to develop an efficient method for cultivating red-topped bamboo fungus that utilizes dead bamboo resources, so as to achieve sustainable resource utilization and increase economic benefits. Summary of the Invention

[0003] To address the aforementioned problems, the present invention aims to provide a method for simulating the wild cultivation of *Dictyophora indicum* using dead bamboo resources. This method can improve the reuse rate of felled bamboo, conserve resources, reduce the planting cost of *Dictyophora indicum*, and achieve sustainable resource utilization and increased economic benefits.

[0004] The objective of this invention is achieved through the following technical solution:

[0005] A method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources includes the following steps: digging trenches downwards in the soil layer between *Phyllostachys edulis* groves; placing at least one water-collecting layer at the bottom of the trench, arranged in a crisscross pattern from bottom to top, each water-collecting layer consisting of several first arc-shaped bamboo strips placed side-by-side with their concave surfaces facing upwards and several second arc-shaped bamboo strips placed with their concave surfaces facing downwards, wherein a second arc-shaped bamboo strip covers the space between two adjacent first arc-shaped bamboo strips, forming a primary water-retaining layer at the bottom of the trench; and laying a lower filling layer composed of bamboo shavings above the water-collecting layer as a secondary water-retaining layer and a water vapor passage. The process involves: laying a middle filling layer, composed of a mixture of chopped bamboo shavings and humus, above the lower filling layer as a third-level water storage layer and nutrient supplement layer; constructing cultivation troughs along the length of the trench on the middle filling layer for the cultivation of *Dictyophora indica*; laying a layer of pre-prepared culture medium in the cultivation trough, then placing *Dictyophora indica* spawn sticks with multiple holes punched around them in the middle of the culture medium, and then evenly scattering the mycelium from inside the spawn sticks onto the surface of the culture medium; covering the middle filling layer and culture medium with an upper filling layer composed of sandy loam; and finally covering the upper filling layer with a covering layer.

[0006] The cultivation trough is a trough-shaped body formed by lateral bamboo tubes, bottom bamboo tubes, and bridging bamboo tubes. Each lateral bamboo tube, bottom bamboo tube, and bridging bamboo tube is obtained by breaking a portion of bamboo along its axial direction to create an axial opening that exposes its interior. There are two lateral bamboo tubes, with their axial openings facing downwards and positioned along the length of the trough on the middle filling layer. There are also two bottom bamboo tubes, positioned between the two lateral bamboo tubes along the length of the trough, with their axial openings facing upwards and embedded in the middle filling layer. The side of each bottom bamboo tube extends to the bottom of the lateral bamboo tube on the same side. The bottom bamboo tubes are filled with water-retaining material that contacts the cultivation medium. The bridging bamboo tubes are positioned above the two bottom bamboo tubes, covering the gap between them, with their axial openings facing downwards. Both sides of the bridging bamboo tubes extend to the inner sides of the two bottom bamboo tubes, contacting the water-retaining material inside.

[0007] The beneficial effects of this invention are as follows:

[0008] (1) High resource utilization rate: make full use of the abundant dead bamboo resources in Tongzi County to realize the recycling of resources and reduce resource waste.

[0009] (2) Significant economic benefits: Using felled bamboo as the main cultivation material reduces production costs, improves economic benefits, and promotes the sustainable development of the square bamboo industry and rural revitalization in Tongzi County.

[0010] (3) Significant ecological benefits: It promotes the reuse of forestry waste, reduces environmental pollution, and has good ecological benefits.

[0011] (4) It can achieve long-term moisturizing and nutritional supplementation: multiple water-retaining layers are constructed using waste bamboo to achieve long-term and stable moisturizing of red-topped bamboo fungus and provide stable nutrition to red-topped bamboo fungus.

[0012] (5) It can produce mushrooms for many years: After making the side wall of the red-topped bamboo fungus stick porous, it is placed in the culture medium of the culture tank. Eventually, the mycelium attaches to the dead bamboo in the culture tank and continuously absorbs the nutrients in the culture tank and the surrounding area to grow continuously, achieving the goal of planting once and growing for many years. Attached Figure Description

[0013] Figure 1 This is a structural diagram of the trench and its internal filling layers and cultivation tank for the simulated wild cultivation of red-topped bamboo fungus using dead bamboo resources as described in this invention.

[0014] Figure 2 This is a cross-sectional view of the water collection layer described in this invention.

[0015] Figure 3 This is a cross-sectional view of the culture tank described in this invention.

[0016] The diagram shows: 1-soil layer, 2-trench, 3-water collection layer, 31-first arc-shaped bamboo strip, 32-second arc-shaped bamboo strip, 4-lower filling layer, 5-middle filling layer, 6-cultivation trough, 61-lateral bamboo tube, 62-bottom bamboo tube, 63-bridging bamboo tube, 7-cultivation material, 8-water-retaining material, 9-red-topped bamboo fungus stick, 10-upper filling layer, 11-covering layer. Detailed Implementation

[0017] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The described embodiments are merely some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] It should be understood that the structures, proportions, sizes, etc., depicted in the accompanying drawings of this specification are merely for illustrative purposes to aid those skilled in the art in understanding and reading the content disclosed herein, and are not intended to limit the conditions under which the invention can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to the size, without affecting the effects and objectives achieved by the invention, should still fall within the scope of the technical content disclosed herein. Furthermore, the terms such as "upper," "lower," "left," "right," and "middle" used in this specification are merely for clarity and are not intended to limit the scope of the invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention's implementation.

[0019] In the description of this invention, it should be noted that, unless otherwise expressly specified and limited, the terms "connected" or "linked" should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral connection; it can refer to a mechanical connection or an electrical connection; it can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. It should be noted that the terms "comprising," "including," or any other variations are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Example

[0020] This embodiment provides a method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources, including the following steps:

[0021] S1. Thin out the bamboo that is too old in the bamboo forest, dig out the bamboo rhizomes and roots, and remove the bamboo branches and leaves from the edges of the bamboo culms. Then, pile up the bamboo culms, bamboo rhizomes, bamboo roots, bamboo branches and bamboo leaves separately for later use.

[0022] S2. Crush bamboo rhizomes, roots, branches, and some cracked bamboo stalks into granules, soak them in water for more than a week to obtain bamboo shavings for later use; mix rotten bamboo leaves from the forest with sandy loam in a 1:3 ratio, add 0.5 times the amount of water, ferment for 1 month, spread them out and sun-dry for 2-3 days to make humus soil for later use.

[0023] S3. Prepare the potting soil. The potting soil is made by composting the following raw materials in the following weight percentages: 62% bamboo shavings, 23% humus, 1% sugar, 1% calcium sulfate, 0.2% potassium dihydrogen phosphate, 0.07% magnesium sulfate, and adjust the pH to 5.1 with 1% citric acid. The moisture content of the potting soil is 70%.

[0024] S4. In July, trench 2 was dug downwards in the soil layer 1 in the gaps of the bamboo forest at an altitude of 1300 meters, as follows: Figure 1 As shown, the cross-section of trench 2 is an inverted trapezoidal structure, and the depth of trench 2 is about 45cm.

[0025] S5. At the bottom of trench 2, two or more water collection layers 3 are stacked crosswise from bottom to top, such as... Figure 1 and Figure 2As shown, each water collection layer 3 is composed of several first arc-shaped bamboo strips 31 placed side by side with their concave surfaces facing upwards and several second arc-shaped bamboo strips 32 placed with their concave surfaces facing downwards. A second arc-shaped bamboo strip 32 covers the space between two adjacent first arc-shaped bamboo strips 31, forming a first-level water storage layer at the bottom of the ditch. The two adjacent first arc-shaped bamboo strips 31 are parallel to each other and have a gap. The second arc-shaped bamboo strip 32 covers the gap between the two adjacent first arc-shaped bamboo strips 31, and the second arc-shaped bamboo strip 32 contacts the top of the two adjacent first arc-shaped bamboo strips 31 directly below it through the bamboo joint on its inner side. At the same time, the two ends of the cross-section of the second arc-shaped bamboo strip 32 extend above the two adjacent first arc-shaped bamboo strips 31 directly below it and are suspended in the air. After this arrangement, the suspended second arc-shaped bamboo strips 31 can block the water vapor evaporating from the soil. After the water vapor rises and touches the inner side of the second arc-shaped bamboo strip 32, it condenses into water droplets and slides along the edge of the second arc-shaped bamboo strip 32 into the first arc-shaped bamboo strip 31 for storage. After the water content of the filling layer above the first arc-shaped bamboo strip 31 decreases, the water in the first arc-shaped bamboo strip 31 can replenish the water in the filling layers above it. After the water content of the filling layer above the first arc-shaped bamboo strip 31 increases, the excess water flows down into the first arc-shaped bamboo strip 31 for storage. Both the first arc-shaped bamboo strip 31 and the second arc-shaped bamboo strip 32 are set along the length of the groove and are obtained by splitting the bamboo pole along the axial direction. The cross-sectional arc of the first arc-shaped bamboo strip 31 is about 2 / 3π to 3 / 2π, and the cross-sectional arc of the second arc-shaped bamboo strip 32 is about 2 / 3π. At the same time, the bamboo joint board on the inner side of the bamboo joint of the first arc-shaped bamboo strip 31 is not removed, thus dividing the interior of the first arc-shaped bamboo strip 31 into multiple natural water storage cavities.

[0026] S6. A lower filling layer 4 composed of bamboo shavings is laid above the (uppermost) water collection layer 3 as a secondary water storage layer and a water vapor channel. The lower filling layer 4 has a water content of over 85%. The interior of the lower filling layer 4 is relatively loose, which facilitates the vertical infiltration of water.

[0027] S7. A middle filling layer 5, made of a mixture of chopped bamboo residue and humus, is laid to the side of the lower filling layer 4 as a third-level water storage layer and nutrient replenishment layer; the moisture content of the middle filling layer is also above 85%. The weight ratio of humus to chopped bamboo residue in the middle filling layer is 1:4.

[0028] S8. A cultivation trough 6 (which can be one or more) is constructed along the length of the groove 2 on the middle filling layer 5 for the cultivation of *Dictyophora indicum*. Figure 1 and Figure 3 As shown, the cultivation trough 6 is a trough-shaped body formed by lateral bamboo tubes 61, bottom bamboo tubes 62 and bridging bamboo tubes 63 and filled with cultivation material 7. The lateral bamboo tubes 61, bottom bamboo tubes 62 and bridging bamboo tubes 63 are all obtained by breaking a part of the bamboo along its axis to form an axial opening to expose its interior.

[0029] The number of lateral bamboo tubes 61 is two, and the axial openings of the two lateral bamboo tubes 61 are downward and arranged on the middle filling layer 5 along the length direction of the groove 2.

[0030] The number of bottom bamboo tubes 62 is two and they are arranged between two lateral bamboo tubes 61 along the length of the groove 2. The axial openings of the two bottom bamboo tubes 62 face upward and are embedded in the middle filling layer 5. The side of each bottom bamboo tube 62 extends to the bottom of the lateral bamboo tube 61 on the same side. The bottom bamboo tubes 62 are filled with water-retaining material 8 that is in contact with the bottom of the culture medium 7.

[0031] The bridging bamboo tube 63 is positioned above the two bottom bamboo tubes 62 and covers the gap between the two bottom bamboo tubes 62, with its axial opening facing downwards. At the same time, the two sides of the bridging bamboo tube 63 extend to the inner sides of the two bottom bamboo tubes 62 and contact the water-retaining material 8 inside the bottom bamboo tubes 62.

[0032] The portion of the middle filling layer 5 located directly below the lateral bamboo tube 61 protrudes upward and enters the lateral bamboo tube 61, while extending into the bottom bamboo tube 62 to contact the water-retaining material 8, but not to contact the inner side of the lateral bamboo tube 61; the portion of the middle filling layer 5 located directly below the bridging bamboo tube 63 protrudes upward and enters the bridging bamboo tube 63, while extending into the bottom bamboo tube 62 to contact the water-retaining material 8, but not to contact the inner side of the bridging bamboo tube 63.

[0033] The water-retaining material 8 is obtained by mixing humus, sandy loam, and expanded bamboo shavings in a ratio of 1:3:6. The expanded bamboo shavings are obtained by expanding bamboo shavings using an expansion machine. The moisture content of the water-retaining material 8 is above 85%.

[0034] S9. A layer of pre-prepared culture medium 7 is laid in the culture tank 6. Then, the red-topped bamboo fungus stick 9 is placed in the middle of the culture medium 7 after multiple holes are punched around its perimeter. The fungal material inside the red-topped bamboo fungus stick 9 is then evenly sprinkled on the surface of the culture medium 7. When the moisture and nutrients in the culture medium 7 decrease, the moisture and nutrients in the water-retaining material 8 will continuously replenish the moisture and nutrients in the culture medium 7 (nutrient water is carried into the culture medium 7 with the flow of water). Similarly, the water in the water-retaining material 8 is replenished in two ways: one is through the middle filling layer 5, and the other is through the water condensed at the bottom of the inner side of the lateral bamboo tube 61 and the bridging bamboo tube 63. This part of the water is the water vapor evaporated from the middle filling layer 5, which condenses into water droplets after hitting the bottom of the inner side of the lateral bamboo tube 61 and the bridging bamboo tube 63 and slides along the edges of the lateral bamboo tube 61 and the bridging bamboo tube 63 into the water-retaining material 8 in the bottom bamboo tube 62. As for the replenishment of nutrients in the water-retaining substrate 8, water is simultaneously introduced into the substrate 8 as it is added from the middle filling layer 5. Similarly, the moisture in the middle filling layer 5 can also be replenished through the lower filling layer 4 and the water collection layer 3, while the moisture in the lower filling layer 4 and the water collection layer 3 is replenished through the bottom soil, thus ensuring the continuity of moisture and maintaining the substrate 7 in a high humidity state. This guarantees the humidity environment and nutrients required for fruiting, ensuring normal fruiting.

[0035] S10. A top filling layer 10 composed of sandy loam is placed on top of the middle filling layer 5 and the culture medium 7. It has good permeability and facilitates the downward infiltration of surface water. The thickness of the top filling layer 10 directly above the culture medium 7 is 3-5 cm.

[0036] S11. Cover the surface of the upper filling layer 10 with a covering layer 11 to block the water vapor evaporating and rising from the soil, so that it re-condenses into water droplets and falls back into the soil; the covering layer 11 is made of bamboo leaves and has a thickness of 2-5cm.

[0037] After cultivation, mushrooms will begin to appear in October. From November to December, the mycelium will attach to the dead bamboo. In March of the following year, the bamboo fungus will grow normally.

[0038] Other aspects of this invention that are not detailed herein are all conventional techniques known to those skilled in the art.

[0039] It should be noted that the terms “comprising,” “including,” or any other variations are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0040] The scope of protection of this invention is not limited to the technical solutions disclosed in the specific embodiments. Any modifications, equivalent substitutions, improvements, etc., made to the above embodiments based on the technical essence of this invention shall fall within the scope of protection of this invention.

Claims

1. A method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources, characterized in that: Includes the following steps, Dig trenches downwards in the soil layer between the bamboo groves; At least one water collection layer is stacked crosswise from bottom to top at the bottom of the trench. Each water collection layer consists of several first arc-shaped bamboo strips placed side by side with their concave surfaces facing upwards and several second arc-shaped bamboo strips placed with their concave surfaces facing downwards. A second arc-shaped bamboo strip covers the space between two adjacent first arc-shaped bamboo strips. A lower filling layer composed of shredded bamboo is laid above the water collection layer; A middle filling layer, made of a mixture of chopped bamboo shavings and humus, is laid on top of the lower filling layer. A culture tank is constructed along the length of the trench on the intermediate filling layer; A layer of pre-prepared culture medium is laid in the culture tank. Then, several holes are punched around the red-topped bamboo fungus sticks and they are placed in the middle of the culture medium. Then, the fungus material inside the red-topped bamboo fungus sticks is evenly sprinkled on the surface of the culture medium. A top filling layer composed of sandy loam is placed over the middle filling layer and the culture medium. A cover layer is placed over the surface of the fill layer.

2. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: In the water collection layer, the curvature of the cross-section of each first arc-shaped bamboo strip is 2 / 3π to 3 / 2π.

3. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: In the water collection layer, two adjacent first arc-shaped bamboo strips are parallel to each other and have a gap. The second arc-shaped bamboo strip covers the gap between the two adjacent first arc-shaped bamboo strips. The second arc-shaped bamboo strip contacts the top of the two adjacent first arc-shaped bamboo strips directly below it through its inner bamboo joint. At the same time, the two ends of the cross-section of the second arc-shaped bamboo strip extend to the top of the two adjacent first arc-shaped bamboo strips directly below it and are suspended in the air.

4. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: The shredded bamboo is obtained by crushing bamboo poles, bamboo whips, bamboo roots and bamboo branches into particles and soaking them in water for more than a week. The humus is obtained by mixing rotten bamboo leaves and sandy loam in a ratio of 1:(2-5).

5. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 4, characterized in that: The weight ratio of humus to bamboo shavings in the intermediate filling layer is 1:(3-5).

6. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: The cultivation trough is a trough-shaped body formed by lateral bamboo tubes, bottom bamboo tubes and bridging bamboo tubes. The lateral bamboo tubes, bottom bamboo tubes and bridging bamboo tubes are all obtained by breaking a part of the bamboo along its axis to form an axial opening to expose its interior. The number of lateral bamboo tubes is two, and the axial openings of the two lateral bamboo tubes face downwards and are set on the middle filling layer along the length of the groove. The bottom bamboo tubes consist of two tubes arranged between two lateral bamboo tubes along the length of the groove. The axial openings of the two bottom bamboo tubes face upwards and are embedded in the middle filling layer. The side of each bottom bamboo tube extends to the bottom of the lateral bamboo tube on the same side. The bottom bamboo tubes are filled with water-retaining material that is in contact with the culture medium. The bridging bamboo tube is positioned above the two bottom bamboo tubes and covers the gap between them, with its axial opening facing downwards. Simultaneously, both sides of the bridging bamboo tube extend to the inner sides of the two bottom bamboo tubes and contact the water-retaining material inside the bottom bamboo tubes.

7. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 6, characterized in that: The middle filling layer, located below the lateral bamboo tube, protrudes upward and enters the lateral bamboo tube, while extending into the bottom bamboo tube to contact the water-retaining material, but not contacting the inner side of the lateral bamboo tube; the middle filling layer, located below the bridging bamboo tube, protrudes upward and enters the bridging bamboo tube, while extending into the bottom bamboo tube to contact the water-retaining material, but not contacting the inner side of the bridging bamboo tube.

8. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 6, characterized in that: The water-retaining material is obtained by mixing humus, sandy soil and expanded bamboo residue in a ratio of 1:(2-5):(5-9).

9. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: The covering layer is made of bamboo leaves and is 2-5 cm thick.

10. The method for simulating wild cultivation of *Dictyophora indicum* using dead bamboo resources according to claim 1, characterized in that: The thickness of the filling layer directly above the culture medium is 3-5 cm.

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