Anti-wrinkle composition, use, skin care product, anti-wrinkle essence cream

Abstract

The present invention belongs to the technical field of skin care, and discloses an anti-wrinkle composition, use, skin care product, and anti-wrinkle essence cream. The anti-wrinkle composition comprises the following components by weight: 1-2 parts of okra hydrolyzed glycoprotein, 10-20 parts of Bidens pilosa extract, 1-2 parts of type XVII collagen, 1-2 parts of type III small molecule collagen, and 6-12 parts of Cordyceps sinensis extract. The present invention discloses an anti-wrinkle composition, which produces a synergistic effect of improving the anti-wrinkle effect of the composition by compounding okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type III small molecule collagen, and Cordyceps sinensis extract; through the compounding of the five, not only the lost collagen is directly supplemented for the skin, but also the collagen production capacity of the skin itself is stimulated, so as to achieve lasting firmness and smoothness of the skin.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to an anti-wrinkle composition, use, skin care product and anti-wrinkle essence cream. Background Art

[0002] Skin aging is a natural process, but as we age, our skin will experience sagging, wrinkles, pigmentation and other problems. These problems not only affect the appearance, but also have a negative impact on a person's mental state. Therefore, skin anti-aging has become an important means for many people to pursue beauty and self-confidence, and the most intuitive manifestation of skin anti-aging is the anti-wrinkle effect.

[0003] As consumers' requirements for the efficacy and safety of skin care products continue to increase, traditional anti-aging products can no longer meet market demand. Traditional anti-aging products often have limited effects, and some products are irritating or have side effects.

[0004] Skin aging is closely related to the loss of collagen. As we age, collagen will gradually be lost, the skin will become loose and aged, and wrinkles will increase. Therefore, many anti-aging skin care products currently use the method of directly supplementing collagen to achieve the purpose of anti-wrinkle and anti-aging.

[0005] As described in prior art 1: Chinese patent application 202411021929.2 discloses a method for preparing and applying an anti-aging composition for the face, comprising the following steps: material preparation step: take the following raw materials by weight: 5-50 parts of hyaluronic acid, 5-50 parts of exosomes, 1-15 parts of botulinum toxin, 2-40 parts of complex antioxidants, 5-20 parts of collagen, 5-10 parts of salicylic acid, 4-10 parts of acetylaspartic acid, 1-20 parts of retinol, 2-10 parts of peptides, 5-15 parts of squalane and 5-30 parts of deionized water.

[0006] The above technology uses collagen, retinol and other components to supplement the skin's collagen and promote cell and collagen regeneration, thereby achieving the effect of anti-wrinkle on the skin. It is a very effective technical solution; however, the facial anti-aging composition of this technical solution needs to be combined with a roller needle or radio frequency to efficiently introduce the effective ingredients into the skin, especially the presence of botulinum toxin, and requires a doctor with sufficient injection experience to complete the operation, which is not suitable for daily use by ordinary consumers.

[0007] Based on this, the technical problem to be solved by the present invention is to develop an anti-wrinkle product that is suitable for daily use by ordinary consumers and has both safety and efficacy, so as to meet consumers' daily anti-aging skin care needs. Summary of the invention

[0008] One of the purposes of the present invention is to provide an anti-wrinkle composition, which produces a synergistic effect of improving the anti-wrinkle effect of the skin by compounding a plurality of highly safe components, has both safety and efficacy, and can be used by ordinary skin care means, and is suitable for daily use by consumers.

[0009] Another object of the present invention is to provide a use of an anti-wrinkle composition, wherein the anti-wrinkle composition is used to prepare skin care products and can meet consumers' daily anti-aging and anti-wrinkle skin care needs.

[0010] At the same time, the present invention also provides a skin care product containing the anti-wrinkle composition of the present invention, which has excellent anti-wrinkle and anti-aging effects.

[0011] Furthermore, the present invention also provides an anti-wrinkle essence cream, which has significant anti-wrinkle, firming, moisturizing and soothing effects through the compounding of multiple components.

[0012] To achieve the above-mentioned object, the present invention provides an anti-wrinkle composition, comprising the following components by weight: 1-2 parts of okra hydrolyzed glycoprotein, 10-20 parts of Bidens pilosa extract, 1-2 parts of type XVII collagen, 1-2 parts of type III small molecule collagen, and 6-12 parts of Cordyceps sinensis extract.

[0013] Okra hydrolyzed glycoprotein is extracted and purified from the viscous liquid in the young okra pods. It can form a "plant hydrogel film" that can retain moisture, isolate pollution, and help increase skin moisture content, soften skin, and regulate skin feel.

[0014] Bidens pilosa L. extract, as a biological retinol, has the effect of promoting collagen production and improving skin elasticity.

[0015] Type XVII collagen is recombinant type XVII collagen, 100% humanized collagen, which can significantly increase dermal thickness and collagen density.

[0016] Type III small molecule collagen is also a recombinant type III small molecule collagen, which has the function of promoting skin collagen production.

[0017] Cordyceps sinensis extract is rich in multiple active ingredients and upregulates the content of type I collagen in HDF cells.

[0018] The present invention ingeniously combines five major ingredients: okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type III small molecule collagen and Cordyceps sinensis extract; Bidens pilosa extract acts as a biological retinol, which effectively stimulates the synthesis of the skin's own collagen and significantly improves skin elasticity.

[0019] At the same time, we have specially added 100% humanized type XVII collagen, which can precisely act on the dermis, significantly thicken the dermis, increase the density of collagen fibers, and improve skin sagging from the root. And type III small molecule collagen, with its tiny molecular structure, can easily penetrate the skin barrier, promote the regeneration of endogenous collagen in the skin, and further consolidate the skin's firm structure.

[0020] In addition, Cordyceps sinensis extract, with its rich active ingredients, can effectively activate the skin's self-repair mechanism, enhance the expression level of type I collagen, and provide comprehensive nutritional support for the skin; the exquisite synergy of these five major ingredients not only directly replenishes the skin's lost collagen, but also stimulates the skin's own collagen production capacity, achieving long-lasting firmness and smoothness of the skin.

[0021] The anti-wrinkle composition of the present invention further comprises water as a solvent.

[0022] The invention also discloses the use of the anti-wrinkle composition in preparing skin care products.

[0023] Meanwhile, the invention also discloses a skin care product containing 1-5wt% of the anti-wrinkle composition.

[0024] Furthermore, the present invention also discloses an anti-wrinkle essence cream, which comprises the following components by mass percentage:

[0025] 1-5% of the above anti-wrinkle composition, 0.1-0.3% of allantoin, 0.3-0.7% of panthenol, 0.05-0.2% of ectoine, 0.5-10% of stabilizer, 0.02-0.08% of thickener, 8-16% of oil, and the balance is water.

[0026] Furthermore, the stabilizer is at least one of ammonium acryloyldimethyltaurate / VP copolymer, carbomer, cetearyl alcohol, and glyceryl stearate.

[0027] Furthermore, the thickener is at least one of xanthan gum, sclerotium gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, and carbomer.

[0028] Furthermore, the oil is at least one of polydimethylsiloxane, caprylic / capric triglyceride, squalane, cetyl alcohol, and behenyl alcohol.

[0029] As a preferred technical solution, the anti-wrinkle essence cream of the present invention further comprises 0.1-10% of an auxiliary agent, wherein the auxiliary agent is at least one of gentian root extract, bisabolol, Centella asiatica extract, and oat β-glucan.

[0030] It should be noted that the present invention can add auxiliary agents according to the needs of efficacy, including but not limited to gentian root extract, bisabolol, Centella asiatica extract, and oat β-glucan.

[0031] Furthermore, the anti-wrinkle cream of the present invention comprises, by weight percentage:

[0032] Phase A: 0.8% ammonium acryloyldimethyltaurate / VP copolymer, 0.05% xanthan gum, 0.2% allantoin, 0.05% okra hydrolyzed glycoprotein, 0.5% panthenol, 3% dipropylene glycol, and the balance water;

[0033] Phase B: 1.5% cetearyl alcohol, 1% glyceryl stearate, 1% C10-18 fatty acid triglycerides, 3% caprylic / capric triglycerides, 1.5% dimethicone, 0.8% cetyl alcohol, 1.2% behenyl alcohol, 0.5% tocopheryl acetate, 1% coconut oil-caprylate / caprate, 1% squalane, 0.5% Bidens pilosa extract;

[0034] Phase C: Gentiana root extract 0.5%, tetrahydromethylpyrimidine carboxylic acid 0.1%, acetyl hexapeptide-8 0.5%, type XVII collagen 0.05%, type III small molecule collagen 0.05%, bisabolol 0.2%, Centella asiatica extract 0.001%, oat β-glucan 0.1%, and Cordyceps sinensis extract 0.3%.

[0035] The present invention further provides a method for preparing the above-mentioned anti-wrinkle essence cream, comprising the following steps:

[0036] Step 1: Add phase A and phase B into water step by step to dissolve, heat to 80-85°C and stir evenly, then homogenize;

[0037] Step 2: Cool down to 40°C with stirring, add the C phase components one by one and stir until completely dissolved;

[0038] Step 3: Continue stirring until a homogeneous emulsion is formed.

[0039] Beneficial Effects

[0040] Compared with the prior art, the present invention has at least the following advantages:

[0041] (1) The present invention discloses an anti-wrinkle composition, which is a combination of okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type III small molecule collagen, and Cordyceps sinensis extract to synergistically improve the anti-wrinkle effect of the composition; the combination of the five ingredients not only directly replenishes the lost collagen for the skin, but also stimulates the skin's own collagen production capacity, thereby achieving lasting firmness and smoothness of the skin;

[0042] (2) The present invention discloses an anti-wrinkle essence cream, which adds allantoin, panthenol, ectoine and other components to the anti-wrinkle composition, and can exert significant anti-wrinkle, firming, moisturizing and soothing effects. DETAILED DESCRIPTION

[0043] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0044] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0045] In the following examples and comparative examples, okra hydrolyzed glycoprotein was purchased from Shanghai Jiayu Biotechnology Co., Ltd. (content 100%); Bidens pilosa extract was purchased from Revinage-Chemyunion Ltda., with the trade name Revinage (the content of Bidens pilosa extract ranged from 12-28wt%); type XVII collagen was purchased from Jiangsu Chuangjian Medical Technology Co., Ltd., with the trade name recombinant XVII Type III collagen, product model TTB01-02SC; type III small molecule collagen was purchased from Jiangsu Chuangjian Medical Technology Co., Ltd., with the trade name of recombinant type III small molecule collagen, product model TTA03-02SC, cordyceps sinensis extract was purchased from Shanghai Jiayu Biotechnology Co., Ltd. (the cordyceps sinensis extract content was 10wt%, and the rest was caprylic / capric triglyceride), Chinese rehmannia root extract was purchased from Shanghai Jiayu Biotechnology Co., Ltd., stevia leaf extract was purchased from Lubrizol Specialty Chemical Manufacturing (Shanghai) Co., Ltd. (the stevia leaf / stem extract content ranged from 3.6-4.4wt%); fibronectin was purchased from Jiangsu Chuangjian Medical Technology Co., Ltd., with the trade name BSP-Reprotein® FN-R; hydrolyzed collagen was purchased from Shanghai Jiayu Biotechnology Co., Ltd., with the trade name of hydrolyzed collagen; ginseng root extract was purchased from ginseng cell tissue extract provided by Dalian Purecon Biotechnology Co., Ltd.

[0046] It should be noted that the technical solution protected by the present invention is not limited to the raw materials of the above-mentioned suppliers, and the technical solution of the present invention can be implemented by other commercially available raw materials.

[0047] In the following examples and comparative examples, unless otherwise specified, the parts and % refer to parts by weight and weight percentage, respectively; meanwhile, water is present as a solvent in the examples and comparative examples of the present invention.

[0048] According to the formula in Table 1, the anti-wrinkle compositions of Examples 1-5 and Comparative Examples 1-10 were prepared by the following preparation methods:

[0049] Step 1: Add okra hydrolyzed glycoprotein or Tremella fuciformis extract, Bidens pilosa extract or Stevia leaf extract into water to dissolve, heat to 85° C. and stir evenly, and then homogenize;

[0050] Step 2: Stir and cool to 40°C, add type XVII collagen or fibronectin, type III small molecule collagen or hydrolyzed collagen, cordyceps extract or ginseng root extract and stir until completely dissolved.

[0051] Table 1 Anti-wrinkle composition formula

[0052] Okra hydrolyzed glycoprotein / % Bidens pilosa L. extract / % Type XVII collagen / % Type III small molecule collagen / % Cordyceps sinensis extract / % Chinese Rehmannia glutinosa root extract / % Stevia leaf extract / % Fibronectin / % Hydrolyzed collagen / % Ginseng root extract / % water / % Example 1 0.05 0.5 0.05 0.05 0.5 0 0 0.05 0 0 Up to 100 Example 2 0.1 0.75 0.1 0.05 0.3 0 0 0 0 0 Up to 100 Example 3 0.07 0.52 0.07 0.07 0.6 0 0 0 0 0 Up to 100 Example 4 0.05 0.5 0.05 0.05 0.3 0 0 0 0 0 Up to 100 Example 5 0.1 1 0.1 0.1 0.4 0 0 0 0 0 Up to 100 Comparative Example 1 0 0.52 0.06 0.06 0.31 0 0 0 0 0 Up to 100 Comparative Example 2 0.11 0 0.11 0.11 0.62 0 0 0 0 0 Up to 100 Comparative Example 3 0.06 0.52 0 0.06 0.31 0 0 0 0 0 Up to 100 Comparative Example 4 0.06 0.52 0.06 0 0.31 0 0 0 0 0 Up to 100 Comparative Example 5 0.07 0.74 0.07 0.07 0 0 0 0 0 0 Up to 100 Comparative Example 6 0 0.5 0.05 0.05 0.3 0.05 0 0 0 0 Up to 100 Comparative Example 7 0.05 0 0.05 0.05 0.3 0 2 0 0 0 Up to 100 Comparative Example 8 0.05 0.5 0 0.05 0.3 0 0 0.05 0 0 Up to 100 Comparative Example 9 0.05 0.5 0.05 0 0.3 0 0 0 0.05 0 Up to 100 Comparative Example 10 0.05 0.5 0.05 0.05 0 0 0 0 0 0.3 Up to 100

[0053] Efficacy Test

[0054] 1. Anti-wrinkle effect test

[0055] Test method: Elastase inhibition rate.

[0056] 1. Test purpose and principle:

[0057] The anti-wrinkle effect is mainly characterized by evaluating the inhibition rate of test samples on elastase. Elastase is mainly synthesized and secreted by fibroblasts, which can degrade elastin in the skin and cause skin aging. The experimental principle of inhibiting elastase is that porcine pancreatic elastase catalyzes the reaction with the enzyme substrate. After adding the active substance, the absorbance changes. The size of the absorbance change reflects the inhibition rate of the elastase inhibitor.

[0058] This test refers to the laboratory method (HMC-WI-028 elastase inhibition rate) and compares the elastase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain anti-wrinkle effect.

[0059] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve anti-wrinkle efficacy by inhibiting elastase.

[0060] 2. Test indicators

[0061] Elastase inhibition rate determination criteria: If the sample elastase inhibition rate is higher than that of the negative control and there is a significant difference (P<0.05), then it can be considered that the test sample has a certain anti-wrinkle effect.

[0062] 3. Experimental Materials and Methods

[0063] 3.1 Instruments and Equipment

[0064] BSA224S analytical balance, RT-6100 ELISA analyzer.

[0065] 3.2 Reagents

[0066] Elastase (porcine pancreas), BR;

[0067] N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0068] Epigallocatechin gallate (EGCG), 98%.

[0069] 3.3 Test methods

[0070] (1) Treatment of control and test samples

[0071] Sample group: 100% of Examples 1-5 and Comparative Examples 1-10;

[0072] Positive control (EGCG): diluted with water to a positive control concentration of 0.1%;

[0073] Negative control: pure water.

[0074] (2) Test operation steps

[0075] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0076] (3) Calculation formula

[0077] ;

[0078] Where: A—is the absorbance of the reaction solution without sample;

[0079] B—is the absorbance of the reaction solution without sample and enzyme;

[0080] C—is the absorbance of the reaction solution containing sample and enzyme;

[0081] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0082] (4) Data analysis

[0083] The statistical analysis software was SPSS, and the comparison of elastase inhibition rates among the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0084] The anti-wrinkle compositions of Examples 1-5 and Comparative Examples 1-10 were tested according to the above method, and the results are shown in Table 2;

[0085] Table 2 Anti-wrinkle efficacy test results of the anti-wrinkle compositions of Examples 1-5 and Comparative Examples 1-10

[0086] Elastase inhibition rate% P-value Example 1 50.83 ＜0.050 Example 2 52.05 ＜0.050 Example 3 54.28 ＜0.050 Example 4 48.56 ＜0.050 Example 5 53.96 ＜0.050 Comparative Example 1 33.49 ＜0.050 Comparative Example 2 35.74 ＜0.050 Comparative Example 3 30.17 ＜0.050 Comparative Example 4 28.99 ＜0.050 Comparative Example 5 31.70 ＜0.050 Comparative Example 6 34.18 ＜0.050 Comparative Example 7 34.89 ＜0.050 Comparative Example 8 36.55 ＜0.050 Comparative Example 9 35.30 ＜0.050 Comparative Example 10 30.68 ＜0.050 Negative control -1.51 / Positive Control 82.23 ＜0.050

[0087] According to the results in Table 2, we can see that:

[0088] According to the data of Examples 1-5, the present invention has excellent anti-wrinkle efficacy when compounded with okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type II small molecule collagen and Cordyceps sinensis extract, and exhibits significant elastase inhibition effect.

[0089] According to the data comparison between Example 4 and Comparative Examples 1-5, under the condition of equal total dosage, when Comparative Examples 1-5 lack any one of the components of okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type II small molecule collagen, and Cordyceps sinensis extract, the elastase inhibition rate of the composition will be greatly reduced, indicating that the present invention produces a synergistic effect of enhancing the anti-wrinkle effect of the composition through the compounding of okra hydrolyzed glycoprotein, Bidens pilosa extract, type XVII collagen, type II small molecule collagen and Cordyceps sinensis extract.

[0090] Similarly, according to the data comparison between Example 4 and Comparative Examples 6-10, it can be seen that Comparative Example 6 uses Chinese Rehmannia root extract to replace okra hydrolyzed glycoprotein. The Chinese Rehmannia root extract is rich in rehmannia polysaccharides and has certain similarities with okra hydrolyzed glycoprotein. However, when the Chinese Rehmannia root extract replaces the okra hydrolyzed glycoprotein, it lacks and cannot produce a synergistic effect with Bidens pilosa extract, type XVII collagen, type II small molecule collagen and Cordyceps sinensis extract, resulting in the elastase inhibition effect being significantly worse than that of Example 4; Similarly, Comparative Example 7 uses stevia leaf extract to replace Bidens pilosa extract, Comparative Example 8 uses fibronectin to replace type XVII collagen, Comparative Example 9 uses hydrolyzed collagen to replace type III small molecule collagen, and Comparative Example 10 uses ginseng root extract to replace Cordyceps sinensis extract. All of these can only show a simple superposition of anti-wrinkle effects, and do not produce a synergistic effect of enhancing the anti-wrinkle effect of the composition like Example 4 of the present invention.

[0091] Application Examples and Comparative Examples

[0092] The preparation methods of the anti-wrinkle cream of Application Example 1 and Application Comparative Examples 1-3 are as follows:

[0093] Step 1: Add phase A and phase B into water step by step to dissolve, heat to 80-85°C and stir evenly, then homogenize;

[0094] Step 2: Cool down to 40°C with stirring, add the C phase components one by one and stir until completely dissolved;

[0095] Step 3: Continue stirring until a homogeneous emulsion is formed.

[0096] In the following application examples and comparative examples, allantoin was purchased from KS-Allantoin-WeylChemLamotte SAS; panthenol was purchased from D-Panthenol USP provided by BASF New Materials Co., Ltd.; tetrahydromethylpyrimidine carboxylic acid (ectoine) was purchased from Shandong Qianpeptide Biotechnology Co., Ltd.; ammonium acryloyldimethyltaurate / VP copolymer was purchased from ARISTOFLEX AVC-Clariant Produkte (Deutschland) GmbH; mevalonate lactone was purchased from NIKKOLMevalution provided by Nikko Chemical Trading (Shanghai) Co., Ltd.; avocado tree fat extract was purchased from Guangzhou Rongdao Chemical Co., Ltd.

[0097] It should be noted that the technical solution protected by the present invention is not limited to the raw materials of the above-mentioned suppliers, and the technical solution of the present invention can be implemented by other commercially available raw materials.

[0098] Application Example 1

[0099] The anti-wrinkle cream comprises, by weight percentage:

[0100] Phase A: 0.8% ammonium acryloyldimethyltaurate / VP copolymer, 0.05% xanthan gum, 0.2% allantoin, 0.05% okra hydrolyzed glycoprotein, 0.5% panthenol, 3% dipropylene glycol, and the balance water;

[0101] Phase B: 1.5% cetearyl alcohol, 1% glyceryl stearate, 1% C10-18 fatty acid triglycerides, 3% caprylic / capric triglycerides, 1.5% dimethicone, 0.8% cetyl alcohol, 1.2% behenyl alcohol, 0.5% tocopheryl acetate, 1% coconut oil-caprylate / caprate, 1% squalane, 0.5% Bidens pilosa extract;

[0102] Phase C: Gentiana root extract 0.5%, tetrahydromethylpyrimidine carboxylic acid 0.1%, acetyl hexapeptide-8 0.5%, type XVII collagen 0.05%, type III small molecule collagen 0.05%, bisabolol 0.2%, Centella asiatica extract 0.001%, oat β-glucan 0.1%, and Cordyceps sinensis extract 0.3%.

[0103] Application Comparative Example 1

[0104] The difference is that hydroxyethyl urea is used instead of allantoin.

[0105] Application Comparative Example 2

[0106] The difference is that shea butter extract is used instead of panthenol.

[0107] Application Comparative Example 3

[0108] The difference is that mevalonolactone is used instead of tetrahydromethylpyrimidine carboxylic acid.

[0109] Efficacy Test

[0110] 1. Anti-wrinkle effect

[0111] Through VISIA and ANTERA 3D image analysis, the skin, wrinkle depth and length were compared before and 8 weeks after use. The results are shown in Table 3. Wrinkle depth change rate % = (wrinkle depth after 8 weeks of use - wrinkle depth before use) / wrinkle depth before use * 100%. The wrinkle length change rate and wrinkle number change rate can refer to the above formula.

[0112] Table 3 Anti-wrinkle test results of the anti-wrinkle cream of application example 1 and application comparative examples 1-3

[0113]

[0114] According to the results in Table 3:

[0115] When comparative examples 1-3 respectively use hydroxyethyl urea instead of allantoin, avocado oil extract instead of panthenol, and mevalonolactone instead of tetrahydromethylpyrimidine carboxylic acid, although they have relatively good anti-wrinkle effects, they are very different from the application examples of the present invention, indicating that the present invention adds allantoin, panthenol, and ectoine to the anti-wrinkle composition to produce a further synergistic effect of enhancing the anti-wrinkle effect of the skin.

[0116] 2. Overall wrinkle removal effect

[0117] To evaluate the product's effect in reducing skin roughness, we selected 18 female volunteers aged 35-55 years with wrinkles around the eyes. The volunteers applied the product on their faces, including the crow's feet area, twice a day for 28 days. Before and after 28 days, skin copies of the crow's feet area were taken and analyzed using PRIMOS technology. We also performed macrophotography and examination of the face before and after application. The evolution of the arithmetic roughness average (Ra) evolution, understood as the average of all heights and depths to the average of R.

[0118] The application example was used to perform a test according to the above method, and the result showed that Ra decreased by -20.4% after 28 days of use of the application example, and the result was statistically significant.

[0119] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle cream, characterized in that: Calculated by mass percentage, it includes the following components: 1-5% anti-wrinkle composition, 0.1-0.3% allantoin, 0.3-0.7% panthenol, 0.05-0.2% ectoine, 0.5-10% stabilizer, 0.02-0.08% thickener, 8-16% oil, and the balance is water; The anti-wrinkle composition is composed of the following components: 1-2 parts of okra hydrolyzed glycoprotein, 10-20 parts of Bidens pilosa extract, 1-2 parts of type XVII collagen, 1-2 parts of type III small molecule collagen, and 6-12 parts of Cordyceps sinensis extract.

2. The anti-wrinkle cream according to claim 1, characterized in that: The stabilizer is at least one of ammonium acryloyldimethyltaurate / VP copolymer, carbomer, cetearyl alcohol, and glyceryl stearate.

3. The anti-wrinkle cream according to claim 1, characterized in that: The thickener is at least one of xanthan gum, sclerotium gum, acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, and carbomer.

4. The anti-wrinkle cream according to claim 1, characterized in that: The oil is at least one of polydimethylsiloxane, caprylic / capric triglyceride, squalane, cetyl alcohol and behenyl alcohol.

5. The anti-wrinkle cream according to any one of claims 1 to 4, characterized in that: The invention also comprises 0.1-10% auxiliary agent, wherein the auxiliary agent is at least one of gentian root extract, bisabolol, centella asiatica extract and oat beta-glucan.

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