A dandelion seedling screening device and screening method for dandelion breeding

Abstract

The present invention relates to the technical field of agricultural breeding, and discloses a seedling screening device and a screening method for dandelion breeding. A seedling screening device for dandelion breeding comprises: a circulating conveying line for conveying an induction culture medium of dandelions; a culture monitoring module, comprising an information acquisition unit and an information processing unit, wherein the information acquisition unit is used to collect characteristic information data of dandelions in the induction culture medium and environmental status information of the corresponding induction culture medium; the information processing unit is used to process the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium, and screen out the induction culture medium that meets the preset culture standard according to the processing. The culture monitoring module collects and processes data of the dandelion induction culture medium on the circulating conveying line, and screens out dandelions that meet the induction culture standard according to the processing, unifies the screening standard, and solves the problem that it is difficult to monitor the induction process in real time by manual means.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural breeding, and in particular to seedling screening equipment and a screening method for dandelion breeding. Background Art

[0002] Dandelion is a plant of the genus Taraxacum in the Asteraceae family. It is a traditional perennial plant with both medicinal and edible properties, a commonly used Chinese medicinal plant for clearing away heat and detoxifying, and is known as the "natural antibiotic" and "queen of herbs" in traditional Chinese medicine. Not only can the whole plant be used as medicine, but it can also be used as food, health food, livestock feed, etc., and the annual consumption is huge. Dandelion resources are relatively abundant, but due to long-term asexual reproduction and the inability to cross-breed, the genetics and biological diversity of dandelions cannot be fully utilized. At the same time, some excellent germplasms are also on the verge of extinction due to species degeneration. Therefore, the innovation of dandelion germplasm resources and the cultivation of new varieties are imperative.

[0003] Polyploid plants have a superposition effect due to the doubling of chromosomes. The most obvious characteristics are relative "giganticity", high plant biomass and secondary metabolites, strong adaptability and stress resistance. Therefore, polyploid breeding can increase the yield of dandelions, improve the quality of dandelion products, and enhance their stress resistance. It is an important way to improve dandelion varieties, which can improve their medicinal value and commercial value, and provide possibilities for the creation of new germplasm and the cultivation of excellent new varieties. However, when dandelion buds are induced by mutagens using the bud immersion method, the following technical problems arise: artificial bud immersion, as the induction time increases, it is difficult to observe the state of dandelion buds in the induction medium for a long time, and everyone's judgment on the induction state is not unified, so the screening standards vary from person to person, which has an adverse effect on successful breeding. Summary of the invention

[0004] The purpose of the present invention is to provide a dandelion seedling screening device and a screening method for breeding, so as to solve the above technical problems.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A dandelion seedling screening device for breeding, comprising:

[0007] A circulating conveying line for conveying dandelion induction culture medium;

[0008] The culture monitoring module includes an information collection unit and an information processing unit, wherein the information collection unit is used to collect characteristic information data of dandelions in the induction culture medium and environmental status information of the corresponding induction culture medium; the information processing unit is used to process the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium, and screen out the induction culture medium that meets the preset culture standard according to the processing;

[0009] A marking module, used for marking and identifying the induction culture medium;

[0010] The speed control module is used to adjust the running speed of the circulating conveyor line.

[0011] As a further technical solution, the information collection unit includes:

[0012] A pH meter, which is disposed in the induction medium and is used to collect pH information data of the culture solution in the induction medium;

[0013] A temperature sensor, which is disposed in the induction culture medium and is used to collect temperature information data of the induction culture medium;

[0014] The image capture component includes an arched support arranged on a circulating conveyor line, a camera is installed at the lower end of the arched support, and the camera is used to collect image information data of dandelions in the induction culture medium when the induction culture medium is transported to the lower end of the arched support along the circulating conveyor line.

[0015] As a further technical solution, the marking module includes:

[0016] An RFID tag, wherein the RFID tag is disposed on an outer wall of the induction culture medium;

[0017] A reader, the reader is arranged on the arch support and is electrically connected to the information processing unit;

[0018] The display terminal is used to display the screening result information of the induction culture medium.

[0019] As a further technical solution, the process of processing the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium includes:

[0020] S1. Monitoring and analyzing the temperature information data and the pH information data in the induction culture medium, and the analysis result indicates whether the induction environment of the induction culture medium is abnormal;

[0021] S2. When the culture medium to be induced with abnormal environment is transported to the bottom of the camera according to the monitoring and analysis results, image information of the corresponding induction culture medium is collected;

[0022] S3, obtaining characteristic parameters of the corresponding induction medium by preprocessing the image information of the induction medium;

[0023] S4, importing the characteristic parameters into the trained recognition model for recognition analysis, and outputting the recognition analysis result to indicate the induction state of the dandelion in the corresponding induction medium;

[0024] S5. Screen out and mark the induction culture medium corresponding to the abnormal dandelion induction state.

[0025] As a further technical solution, the process of monitoring and analyzing the temperature information data and the pH information data in the induction culture medium includes:

[0026] Divide the pH monitoring process into n intervals ;

[0027] Divide the temperature monitoring interval into m intervals

[0028] (1);

[0029] (2);

[0030] (3);

[0031] In the formula, is the pH value of the culture medium in the induction medium changing with time. is the change of the culture medium temperature in the induction medium over time; is the standard curve of the acid-base change of the culture medium. is the standard curve of culture medium temperature change; , is the preset stage weight coefficient; , is the preset normalization coefficient;

[0032] The abnormal risk value of the induction culture medium is calculated by formula (1) to (3) , and the abnormal risk value With the preset standard value To compare:

[0033] like , then it is judged that the induction environment of the corresponding induction medium is normal;

[0034] like , then it is judged that the induction environment of the corresponding induction culture medium is abnormal.

[0035] As a further technical solution, the process of preprocessing the image information of the induction culture medium includes:

[0036] Adjust the brightness, contrast and color parameters of the image according to unified preset standards, and use filters to remove noise from the image;

[0037] Use Sobel edge detection to segment the dandelion outline from the image;

[0038] The morphological features, color features and texture features of the dandelion are collected based on the dandelion outline, and the morphological features, color features and texture features of the dandelion are parameterized to obtain corresponding feature parameters.

[0039] As a further technical solution, the process of identifying and analyzing the characteristic parameters includes:

[0040] (4);

[0041] (5);

[0042] In formulas (4)~(5), is the preset weight vector; is the characteristic parameter vector of dandelion in the induction medium; is the preset bias; is the sigmoid function;

[0043] The probability value of abnormal dandelion induction in the induction medium was obtained by logistic regression calculation using formulas (4) to (5). , and compare the probability value with the preset classification threshold To compare:

[0044] like , then it is judged that the dandelion in the corresponding induction medium is in a normal induction state;

[0045] like , then the dandelions in the corresponding induction medium are judged to be in an abnormal induction state, and the induction medium is screened out and marked.

[0046] A method for screening dandelion seedlings for breeding, the method comprising the following steps:

[0047] Step 1: Identify and evaluate dandelion resources;

[0048] Step 2: Inducing and culturing dandelion mutants in a plurality of induction culture media; and collecting characteristic information data of the dandelions in the induction culture media and environmental status information of the corresponding induction culture media during the induction process;

[0049] Step three: Process the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium, and screen out the induction culture medium that meets the preset culture standard based on the processing.

[0050] Beneficial effects of the present invention:

[0051] The present invention combines a circulating conveying line and a culture monitoring module. The culture monitoring module collects and processes data of the dandelion induction culture medium on the circulating conveying line, and screens out dandelions that meet the induction culture standards based on the processing, thereby unifying the screening standards and solving the problem that it is difficult to monitor the induction process in real time by manual means. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] The present invention will be further described below in conjunction with the accompanying drawings.

[0053] Figure 1 This is a schematic diagram of the overall structure of the seedling screening device for dandelion breeding in the present invention;

[0054] Figure 2 It is a partial structural schematic diagram of the seedling screening device for dandelion breeding in the present invention;

[0055] Figure 3 A flowchart of processing characteristic information data of dandelion and environmental status information of corresponding induction culture medium in the present invention;

[0056] Figure 4 The present invention is a flow chart of the method for screening dandelion seedlings for breeding.

[0057] Figure numerals: 1. circulating conveying line; 101. induction culture medium; 102. speed control module; 2. image capture component; 201. arch support; 202. camera. DETAILED DESCRIPTION

[0058] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] See also Figure 1~2 As shown, a dandelion seedling screening device for breeding includes:

[0060] The circulating conveyor line 1 is used to convey the induction culture medium 101 of dandelions, and a plurality of induction culture mediums 101 can be placed on the circulating conveyor line 1. The culture monitoring module includes an information acquisition unit and an information processing unit, wherein the information acquisition unit is used to acquire characteristic information data of dandelions in the induction culture medium 101 and environmental status information corresponding to the induction culture medium 101, wherein the characteristic information data can be obtained from the image information of the induction culture medium 101, and the environmental status information includes the temperature and pH value in the induction culture medium 101. The information acquisition unit includes a pH meter, a temperature sensor and a camera 202, wherein the pH meter and the temperature sensor are arranged in the induction culture medium 101 and establish communication with the information processing unit, and the camera 202 for capturing image information is arranged on the circulating conveyor line 1. Since the conveying path of the induction culture medium 101 is cyclic, only one camera 202 is required, and the camera 202 is electrically connected to the information processing unit; the information processing unit is used to process the characteristic information data of the dandelion and the environmental status information of the corresponding induction culture medium 101, and screen out the induction culture medium 101 that meets the preset culture standard according to the processing; the marking module is used to mark and identify the induction culture medium 101, and the marking information includes the initial information of the induction culture medium 101 and the screening information after screening. The speed control module 102 specifically includes a servo motor and a controller for driving the circulating conveyor line 1, which can adjust the running speed of the circulating conveyor line 1 and control the start and stop of the circulating conveyor line 1, for example, when capturing image information, the circulating conveyor line 1 is controlled to pause.

[0061] Through the above technical scheme, this embodiment provides a seedling screening device for dandelion breeding, which collects and processes data of the dandelion induction culture medium 101 on the circulating conveyor line 1 through the culture monitoring module, and screens out dandelions that meet the induction culture standards based on the processing, unifies the screening standards, and solves the problem that it is difficult to monitor the induction process in real time by manual means.

[0062] The information collection unit comprises:

[0063] The pH meter is arranged in the induction culture medium 101 and is used to collect information data on the pH value of the culture solution in the induction culture medium 101. It should be noted that the pH meter is installed at an appropriate position in the culture solution to ensure that the electrode can fully contact the culture solution and measure its pH value, and at the same time, avoid direct contact between the electrode and the wall or bottom of the culture container to reduce errors.

[0064] A temperature sensor, which is disposed in the induction culture medium 101 and is used to collect temperature information data of the induction culture medium 101;

[0065] See also Figure 2As shown, the image capture component 2 includes an arched support 201 arranged on the circulating conveyor line 1, and a camera 202 is installed at the lower end of the arched support 201. The camera 202 is used to collect image information data of dandelions in the induction culture medium 101 when the induction culture medium 101 is transported to the lower end of the arched support 201 along the circulating conveyor line 1.

[0066] Through the above technical scheme, this embodiment provides the composition of the information acquisition unit, wherein the pH meter and the temperature sensor are both arranged in the induction culture medium 101, and are respectively used to collect the pH information data and the temperature information data of the induction culture liquid, and the image capture component 2 is arranged on the circulating conveyor line 1, and can capture images of the induction culture medium 101 transported by the circulating conveyor line 1 to the lower end of the camera 202.

[0067] The marking module comprises:

[0068] RFID tag, the RFID tag is set on the outer wall of the induction culture medium 101, and the reader is set on the arch support 201 and is electrically connected to the information processing unit. It should be noted that the RFID tag is a read-write tag, and the reader has the authority to change the tag information. When dandelions with abnormal induction culture status are screened out, the RFID tag information on the corresponding induction culture medium 101 can be changed to achieve the purpose of screening out the mark. The display terminal is used to display the screening result information of the induction culture medium 101.

[0069] Through the above technical solution, this embodiment provides the composition of the marking module. Through the setting of the RFID tag and the reader, each induction culture medium 101 can be distinguished, and the induction culture medium 101 with abnormal dandelion induction status can be marked.

[0070] See also Figure 3 As shown, the process of processing the characteristic information data of dandelion and the environmental status information of the corresponding induction culture medium 101 includes:

[0071] S1. Monitor and analyze the temperature information data and pH information data in the induction medium 101. The analysis results indicate whether the induction environment of the induction medium 101 is abnormal. The pH of the induction medium 101 is one of the key factors affecting cell growth, which directly affects the progress of cell metabolic activities. When the pH of the culture medium is abnormal, cell growth may be inhibited or even cause cell death. In addition, the appropriate temperature is also a necessary condition for the smooth progress of the induction process.

[0072] S2. When the culture medium with abnormal induction environment is transported to the bottom of the camera 202 according to the monitoring and analysis results, the image information of the corresponding induction culture medium 101 is collected. The abnormal induction environment provided by the induction culture medium 101 has a great influence on dandelion induction. For example, the change of temperature will affect the activity of intracellular enzymes, thereby affecting the metabolic rate of cells. If the temperature is too high or too low, it may cause abnormal cell metabolism, thereby affecting the growth and reproduction of cells. The change of pH will affect the permeability of cell membranes and the activity of intracellular enzymes, thereby affecting the growth and reproduction of cells. If the pH is too high or too low, it may cause cell growth to be inhibited. Therefore, when the induction environment is abnormal, it is necessary to perform further image recognition analysis on the induction state of dandelions.

[0073] S3, obtaining characteristic parameters of the corresponding induction culture medium 101 by preprocessing the image information of the induction culture medium 101;

[0074] S4, importing the characteristic parameters into the trained recognition model for recognition analysis, and outputting the recognition analysis result to indicate the induction state of the dandelion in the corresponding induction medium 101;

[0075] S5. Screen out and mark the induction culture medium 101 corresponding to the abnormal dandelion induction state, which is specifically achieved by changing the tag information of the RFID tag on the corresponding induction culture medium 101 by a reader.

[0076] Through the above technical solution, this embodiment provides a specific process for processing the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium 101.

[0077] The process of monitoring and analyzing the temperature information data and the pH information data in the induction culture medium 101 includes:

[0078] Since the pH and temperature of the culture medium have different effects on the induced culture at different induction stages, the pH monitoring process is divided into n intervals based on this difference. , and divide the temperature monitoring interval into m intervals ; The above differences are specifically reflected in: for example, in the early stage of induction culture, the pH of the culture medium is crucial to the adaptation and growth of cells. The appropriate pH helps cells quickly adapt to the new culture environment and start normal metabolism and growth activities. In the middle of induction culture, cells begin to enter the logarithmic growth phase, at which time the pH of the culture medium has a more significant effect on the growth rate and metabolic activity of cells. In the later stage of induction culture, cells may enter the stable phase or the decay phase, at which time the pH of the culture medium has relatively little effect on the growth and metabolic activities of cells.

[0079] (1);

[0080] (2);

[0081] (3);

[0082] In the formula, is the pH value of the culture medium in the induction medium 101 that changes over time, is the change in the culture medium temperature in the induction medium 101 over time. is the standard curve of the acid-base change of the culture medium. It is a standard curve of the change of culture medium temperature, which can be obtained through the experimental process of standard induction culture. , is the preset stage weight coefficient, which can be obtained by fitting the experimental data; , is the preset normalization coefficient.

[0083] The abnormal risk value of induction medium 101 is calculated by formula (1) to (3) , and the abnormal risk value With the preset standard value Compare: If , then it is judged that the induction environment of the corresponding induction medium 101 is normal; if , it is judged that the induction environment of the corresponding induction culture medium 101 is abnormal.

[0084] Through the above technical solution, this embodiment provides a specific process for monitoring and analyzing the temperature information data and the pH information data in the induction culture medium 101.

[0085] The process of preprocessing the image information of the induction culture medium 101 includes:

[0086] Adjust the brightness, contrast and color parameters of the image according to the unified preset standards to make the details in the image clearer and facilitate subsequent feature extraction. Use filters to remove noise in the image, such as Gaussian noise, salt and pepper noise, etc., to improve image quality.

[0087] The Sobel edge detection is used to segment the dandelion outline from the image; based on the dandelion outline, the morphological features, color features and texture features of the dandelion are collected. The morphological features include the shape, size, protrusions and other features of the dandelion tissue, as well as the structural features of the tissue. Color features: Calculate the pixel distribution of different color components in the image, extract color histograms, color moments and other features to describe the color composition of the image; Texture features: Use gray-level co-occurrence matrix, local binary pattern, wavelet transform and other methods to extract the texture features of the image, and describe the spatial distribution and change law of the pixel gray level in the image. And the morphological features, color features and texture features of the dandelion are parameterized to obtain the corresponding feature parameters. The process of feature parameterization: For simple features, they can be directly described by mathematical formulas or expressions. For example, the morphological feature parameters can be taken as the coverage area and hue of the dandelion tissue. For some complex features, the corresponding parameters can be obtained by transformation or calculation. For example, the edge detection algorithm extracts the edge features of the image, and then uses the edge coordinates, length, direction and other parameters to describe it.

[0088] Through the above technical solution, this embodiment provides a process for preprocessing the image information of the induction culture medium 101.

[0089] The process of identifying and analyzing characteristic parameters includes:

[0090] (4);

[0091] (5);

[0092] In formulas (4)~(5), is the preset weight vector; To induce the characteristic parameter vector of dandelions in the culture medium 101, parameters can be taken to represent the morphological characteristics, color characteristics and texture characteristics of the dandelions respectively. Specifically, the characteristics can be nonlinearly transformed using methods such as kernel functions and neural networks to obtain a more expressive characteristic parameter vector. is the preset bias; is a sigmoid function; the preset weight vector and the preset bias can be obtained through the gradient descent algorithm, which will not be described in detail here.

[0093] The probability value of abnormal dandelion induction in the induction medium 101 was obtained by logistic regression calculation using formula 4~5 , and compare the probability value with the preset classification threshold Compare: If , then it is judged that the dandelion in the corresponding induction medium 101 is in a normal induction state; if , it is determined that the dandelion in the corresponding induction medium 101 is in an abnormal induction state, and the induction medium 101 is screened out and marked. Through the above technical solution, this embodiment provides a process for identifying and analyzing characteristic parameters. It should also be noted that the classification threshold It can be obtained through the F1Score method, which takes into account the precision and recall rate, avoiding the one-sidedness that may be caused by a single feature indicator.

[0094] See also Figure 4 As shown, a method for screening dandelion seedlings for breeding comprises the following steps:

[0095] Step 1: Identify and evaluate dandelion resources. Specifically, establish a resource garden (resource library). Compare and evaluate the existing dandelion germplasm resources in terms of morphology, growth characteristics, adaptability, yield, functional component content, etc., establish a corresponding database, and provide suggestions for further development and utilization. A method combining morphological characteristics, karyotype characteristics and high-performance liquid chromatography fingerprints of main effective components is adopted to classify and evaluate the utilization of dandelion resources. Step 2: Induce and culture dandelion mutants in several induction culture media 101; and collect characteristic information data of dandelions in the induction culture media 101 and environmental status information of the corresponding induction culture media 101 during the induction process. The characteristic information of dandelions includes morphological characteristics, color characteristics and texture characteristics. The environmental status information of the induction culture media 101 includes the temperature and pH of the culture medium. Step 3: Process the characteristic information data of dandelions and the environmental status information of the corresponding induction culture media 101, and screen out the induction culture media 101 that meet the preset culture standards according to the processing. Through the above technical scheme, this embodiment provides a method for screening seedlings for dandelion breeding, in which data of the dandelion induction culture medium 101 on the circulating conveyor line 1 is collected and processed by the culture monitoring module, and then dandelions that meet the induction culture standards are screened out according to the processing, thereby unifying the screening standards and solving the problem that it is difficult to monitor the induction process in real time by manual means.

[0096] The above is a detailed description of an embodiment of the present invention, but the content is only a preferred embodiment of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A dandelion seedling screening device for breeding, characterized in that: include: A circulating conveying line (1) for conveying a dandelion induction culture medium (101); A cultivation monitoring module, comprising an information collection unit and an information processing unit, wherein the information collection unit is used to collect characteristic information data of dandelions in an induction culture medium (101) and environmental status information of the corresponding induction culture medium (101); the information processing unit is used to process the characteristic information data of the dandelions and the environmental status information of the corresponding induction culture medium (101), and screen out the induction culture medium (101) that meets the preset cultivation standard according to the processing; A marking module, used for marking and identifying the induction medium (101); A speed control module (102) for adjusting the running speed of the circulating conveying line (1); The information collection unit comprises: A pH meter, the pH meter being arranged in the induction culture medium (101) and used for collecting pH information data of the culture fluid in the induction culture medium (101); A temperature sensor, the temperature sensor being arranged in the induction culture medium (101) and being used to collect temperature information data of the induction culture medium (101); An image capturing component (2) comprises an arch support (201) arranged on the circulating conveyor line (1), a camera (202) being installed at the lower end of the arch support (201), the camera (202) being used to collect image information data of dandelions in the induction culture medium (101) when the induction culture medium (101) is transported to the lower end of the arch support (201) along the circulating conveyor line (1); The marking module comprises: An RFID tag, wherein the RFID tag is arranged on the outer wall of the induction culture medium (101); A reader, the reader being arranged on the arch support (201) and being electrically connected to the information processing unit; A display terminal, used for displaying screening result information of the induction culture medium (101); The process of processing the characteristic information data of dandelion and the environmental status information of the corresponding induction culture medium (101) includes: S1, monitoring and analyzing the temperature information data and the pH information data in the induction culture medium (101), wherein the analysis result indicates whether the induction environment of the induction culture medium (101) is abnormal; S2, when the culture medium to be induced with abnormal environment is transported to the bottom of the camera (202) according to the monitoring and analysis results, image information of the corresponding induction culture medium (101) is collected; S3, obtaining characteristic parameters of the corresponding induction culture medium (101) by preprocessing the image information of the induction culture medium (101); S4, importing the characteristic parameters into the trained recognition model for recognition analysis, and outputting the recognition analysis result to indicate the induction state of the dandelion in the corresponding induction medium (101); S5, screening out and marking the induction culture medium (101) corresponding to the abnormal dandelion induction state; The process of monitoring and analyzing the temperature information data and the pH information data in the induction culture medium (101) includes: Divide the pH monitoring process into n intervals ; Divide the temperature monitoring interval into m intervals ; （1）； （2）； （3）； In the formula, is the pH value of the culture medium in the induction medium (101) changing with time, is the change in the temperature of the culture medium in the induction medium (101) over time; is the standard curve of the acid-base change of the culture medium. is the standard curve of culture medium temperature change; , is the preset stage weight coefficient; , is the preset normalization coefficient; The abnormal risk value of the induction medium (101) is calculated by formula (1) to (3): , and the abnormal risk value With the preset standard value To compare: like , then it is judged that the induction environment of the corresponding induction medium (101) is normal; like , then it is judged that the induction environment of the corresponding induction culture medium (101) is abnormal.

2. The dandelion seedling screening device for breeding according to claim 1, characterized in that: The process of preprocessing the image information of the induction culture medium (101) includes: Adjust the brightness, contrast and color parameters of the image according to unified preset standards, and use filters to remove noise from the image; Use Sobel edge detection to segment the dandelion outline from the image; The morphological features, color features and texture features of the dandelion are collected based on the dandelion outline, and the morphological features, color features and texture features of the dandelion are parameterized to obtain corresponding feature parameters.

3. The dandelion seedling screening device for breeding according to claim 2, characterized in that: The process of identifying and analyzing characteristic parameters includes: （4）； （5）； In formulas (4)~(5), is the preset weight vector; is the characteristic parameter vector of dandelion in the induction medium (101); is the preset bias; is the sigmoid function; The probability value of abnormal dandelion induction in the induction medium (101) was obtained by logistic regression calculation using formulas (4) to (5). , and compare the probability value with the preset classification threshold To compare: like , then it is judged that the dandelion in the corresponding induction medium (101) is in a normal induction state; like , then the dandelion in the corresponding induction culture medium (101) is judged to be in an abnormal induction state, and the induction culture medium (101) is screened out and marked.

4. A method for screening dandelion seedlings for breeding, characterized in that: The method is applied to the dandelion seedling screening device for breeding as claimed in any one of claims 1 to 3, and the method comprises the following steps: Step 1: Identify and evaluate dandelion resources; Step 2: Inducing and culturing dandelion mutants in a plurality of induction culture media (101); and collecting characteristic information data of the dandelions in the induction culture media (101) and environmental status information of the corresponding induction culture media (101) during the induction process; Step 3: Process the characteristic information data of dandelions and the environmental status information of the corresponding induction culture medium (101), and screen out the induction culture medium (101) that meets the preset culture standard based on the processing.

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