Stent for sample tube

Abstract

The invention relates to a holder for a sample tube. An apparatus for holding a sample tube for sample preparation is provided. The apparatus includes a sample tube holder having an opening to receive a sample tube. The apparatus includes a hinge assembly having a hinge plate and a hinge support, where the hinge plate is movable relative to the hinge support. In one example, the hinge plate pivots in two rotational directions and slides in two translational directions relative to the hinge support. The hinge plate limits vertical movement of the sample tube within the sample tube holder. In one aspect, a method includes inserting a sample tube into an opening of a sample tube holder, and pivoting and sliding a hinge plate relative to a hinge support, where the hinge plate limits vertical movement of the sample tube within the sample tube holder.

Description

[0001] This application is a divisional application. The application date of the original application is December 10, 2019, the application number is 2019800825452, and the name of the invention is “Bracket for sample tubes”.

[0002] CROSS-REFERENCE TO RELATED APPLICATIONS

[0003] This application claims the benefit of U.S. Provisional Application No. 62 / 779,926, filed on December 14, 2018, and U.S. Provisional Application No. 62 / 892,263, filed on August 27, 2019, both of which are incorporated herein by reference in their entirety. Technical Field

[0004] The technology described herein generally relates to a rack for holding samples and various reagents. The technology more specifically relates to a sample tube holder that receives and retains complementary sample tubes, each of which contains a sample for performing a predetermined processing operation using one or more reagents, such as preparing a biological sample for amplifying and detecting polynucleotides extracted from the sample. Background Art

[0005] The medical diagnostic industry is a key factor in today's health care foundation. However, at present, no matter what route, diagnostic analysis has become a bottleneck in patient care. For this, there are several reasons. First, many diagnostic analyses are only carried out with highly professional equipment, which is expensive and can only be operated by trained clinicians. Such equipment is only found in some areas-often only one in any given urban area. This means that most hospitals need to send samples to these places for analysis, thus generating transportation costs and transportation delays, and may even cause sample loss or incorrect operation. Second, the equipment in question is generally not available 'when needed', but is run in batches, so processing time is delayed for many samples because they must wait for the machine to be filled before they can run.

[0006] Understand that sample flow is decomposed into several key steps, it is desirable to consider as much automated mode as possible.For example, once extracted from the patient, the biological sample must be placed in a form suitable for generally involving the use of amplification methods (including but not limited to polymer chain reaction (PCR), TMA, SDA, NASBA, LCR and Rolling-Cycle amplification) to amplify the target carrier (such as nucleotide).Once amplified, the presence of the target nucleotide from the sample needs to be clearly determined.Preparing samples for PCR is currently a time-consuming and labor-intensive step, although no one needs specialized skills, and can be usefully automated.By contrast, steps such as PCR and nucleotide detection have only been generally within the scope of specially trained individuals exposed to specialized equipment.

[0007] In part, due to the quantity of required reagent and the needs for a plurality of liquid transfer (for example, pipetting) operations, sample preparation is labor-intensive. In addition, required reagent has that they need to carry and can obtain enough diversity from different manufacturers usually with different from each other. Even if reagent can be collected together in a single holder and for use ready situation (such as applying for U.S. Patent Application No. 12 / 218, described in 416, the application was submitted on July 14, 2008 (and title is " Reagent Tube, Reagent Holder, and Kits Containing Same (reagent tube, reagent holder and the kit comprising it) ", with the names of people such as Wilson), and is incorporated herein by reference), will be useful, can be provided with the sample tube used in batches and the quantity of reagent, and make them available for the liquid dispensing tool that can operate sample tube and reagent holder in parallel. U.S. Patent Application No. 15 / 017977, filed on February 8, 2016 and entitled “Rack for Sample Tubes and Reagent Holders” in the name of Duffy et al., is also incorporated herein in its entirety.

[0008] The discussion of the background art herein is included to explain the scope of the technology described herein. This is not to be taken as an admission that any of the material referred to was published, known, or part of the common general knowledge as at the priority date of any claim.

[0009] Throughout the description and claims of the specification, the word "comprise" and variations thereof (such as "includes" and "comprising") are not intended to exclude other additives, components, integers or steps. Summary of the invention

[0010] In some embodiments, a device for holding a sample tube is provided. The device can include a sample tube holder, the sample tube holder comprising an opening configured to receive a sample tube. The device can include a hinge assembly, the hinge assembly comprising a hinge plate and a hinge support. In some embodiments, the hinge plate is configured to slide and pivot between a first configuration and a second configuration relative to the hinge support. In some embodiments, the hinge plate is positioned to allow the sample tube to be inserted into the opening in the first configuration. In some embodiments, the hinge plate is positioned to limit the vertical movement of the sample tube in the sample tube holder in the second configuration.

[0011] In some embodiments, the device can include a sliding lock coupled to the hinge plate. In some embodiments, the sliding lock is configured to slide and pivot relative to a hinge pin, wherein the hinge pin is coupled to the hinge support. In some embodiments, the device can include a spring configured to bias the sliding lock against an inner surface of the hinge support. In some embodiments, the device can include a reagent housing. In some embodiments, the sample tube holder and the reagent housing are coupled. In some embodiments, the hinge support and the reagent housing are coupled. In some embodiments, the sample tube holder includes a plurality of openings configured to receive a plurality of sample tubes. In some embodiments, the sample tube holder includes two openings configured to receive the sample tubes. In some embodiments, when the sample tube is received in the sample tube holder, the opening constrains the sample tube in a horizontal direction. In some embodiments, the hinge plate is configured to limit the vertical movement of the sample tube in the sample tube holder. In some embodiments, the hinge plate includes a smaller notch along the edge of the hinge plate, wherein the smaller notch is configured to be placed on the cap of the sample tube. In some embodiments, the hinge plate includes a larger notch along an edge of the hinge plate, wherein the larger notch is configured to allow a sample tube to be removed from or inserted into the sample tube holder. In some embodiments, the device can include the sample tube. In some embodiments, the device can include a self-locking lug positioned relative to the hinge plate.

[0012] In some embodiments, a method is provided. The method can include pivoting a hinge plate relative to a hinge support. The method can include sliding the hinge plate relative to the hinge support once pivoted, wherein the hinge plate is configured to limit vertical movement of the sample tube within the sample tube holder.

[0013] In some embodiments, the hinge plate slides under the biasing force of the spring. In some embodiments, when the sample tube is inserted, the top surface of the hinge plate is rotated away from the vertical axis of the sample tube, and wherein the hinge plate is pivoted to be approximately perpendicular to the vertical axis of the sample tube. The method can include inserting a pipette tip into the sample tube while the sample tube is in the sample tube holder and the hinge plate has pivoted and slid. The method can include removing a pipette tip from the sample tube while the sample tube is in the sample tube holder and the hinge plate has pivoted and slid.

[0014] In some embodiments, a device for holding a sample tube is provided. The device can include a sample tube holder, the sample tube holder comprising an opening configured to receive a sample tube. The device can include a hinge assembly, the hinge assembly comprising a hinge plate and a hinge support. In some embodiments, the hinge plate is configured to slide and pivot between a first configuration and a second configuration relative to the hinge support. In some embodiments, the hinge plate is positioned to allow insertion of the sample tube into the opening in the first configuration. In some embodiments, the hinge plate is positioned to limit vertical movement of the sample tube within the sample tube holder in the second configuration.

[0015] In some embodiments, the device can include a reagent housing, the reagent housing comprising a slot configured to receive a reagent holder. In some embodiments, the opening is arranged in a straight line with the slot. In some embodiments, the sample tube holder and the reagent housing are coupled. In some embodiments, the hinge support and the reagent housing are coupled. In some embodiments, the sample tube holder comprises a plurality of openings configured to receive a plurality of sample tubes. In some embodiments, the sample tube holder comprises two openings configured to receive the sample tube. In some embodiments, when the sample tube is received in the sample tube holder, the opening constrains the sample tube in a horizontal direction. In some embodiments, the hinge plate is configured to apply a force that limits the vertical movement of the sample tube in the sample tube holder. In some embodiments, the device is configured to be received in a receiving compartment comprising a partition, and wherein when the device is received in the receiving compartment, the hinge plate is pivoted by the partition. In some embodiments, the hinge plate comprises a notch along the edge of the hinge plate, wherein the notch is configured to abut the cap of the sample tube. In some embodiments, the hinge plate is pivoted when the device is inserted into a diagnostic device. In some embodiments, the device can include the sample tube.

[0016] In some embodiments, a method is provided. The method can include inserting a sample tube into an opening of a sample tube holder. The method can include pivoting a hinge plate relative to a hinge support, wherein the hinge plate is configured to limit vertical movement of the sample tube within the sample tube holder.

[0017] In some embodiments, pivoting the hinge plate further comprises inserting the sample tube holder into a diagnostic device. In some embodiments, pivoting the hinge plate further comprises contacting the hinge plate with a divider in a receiving compartment of the diagnostic device. In some embodiments, the method can include inserting a pipette tip into the sample tube while the sample tube is in the sample tube holder. In some embodiments, the method can include removing a pipette tip from the sample tube while the sample tube is in the sample tube holder.

[0018] In some embodiments, a method is provided, comprising: inserting a sample tube into an opening of a sample tube holder; pivoting a hinge plate relative to a hinge support; sliding the hinge plate relative to the hinge support once pivoted, wherein the hinge plate is configured to limit vertical movement of the sample tube within the sample tube holder. The hinge plate slides under the biasing force of a spring. When the sample tube is inserted, the top surface of the hinge plate is rotated away from the vertical axis of the sample tube, and wherein the hinge plate is pivoted to be approximately perpendicular to the vertical axis of the sample tube. The method further comprises inserting a pipette tip into the sample tube while the sample tube is within the sample tube holder and the hinge plate has pivoted and slid. The method further comprises removing a pipette tip from the sample tube while the sample tube is within the sample tube holder and the hinge plate has pivoted and slid. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A front perspective view of a first embodiment of a rack for sample tubes and reagent holders is shown.

[0020] Figure 2 Shows Figure 1 Rear perspective view of the bracket.

[0021] Figure 3 Shows Figure 1 Top view of the bracket.

[0022] Figure 4 Shows Figure 1 Side view of the bracket.

[0023] Figure 5 Shows Figure 1 Front view of the bracket.

[0024] Figure 6 Shows Figure 1 Rear view of the bracket.

[0025] Figure 7 Shows Figure 1 Front perspective view of the sample tube holder of the rack.

[0026] Figure 8 Shows Figure 7 Rear perspective view of the sample tube holder.

[0027] Fig. 9 Shows Figure 1 A front perspective view of a hinge assembly of a bracket.

[0028] Fig. 10A Shows Fig. 9 A rear perspective view of the hinge assembly.

[0029] Fig. 10B Shows Fig. 9 Exploded rear view of the hinge assembly.

[0030] Fig.11A Shows Fig. 9 A first side view of a hinge assembly.

[0031] Fig. 11B Shows Fig. 9 A second side view of the hinge assembly.

[0032] Fig.12 Shows Fig. 9 Top view of the hinge assembly.

[0033] Fig.13 Shows Fig. 9 An exploded front perspective view of a portion of a hinge assembly.

[0034] Fig.14 Shows Fig. 9 An exploded front perspective view of a hinge assembly.

[0035] Figures 15A-15C Shows Figure 1 View of the sample tube holder of the rack.

[0036] Figures 16A-16B Shows Figure 1 View of the sample tube holder of the rack.

[0037] Fig.17 shows the diagnostic equipment and Figure 1 Front perspective view of the bracket.

[0038] Fig.18 A front perspective view of the diagnostic device is shown.

[0039] Figures 19A-19B A front perspective view of a second embodiment of a rack for sample tubes and reagent holders is shown.

[0040] Figures 20A-20B Shows Fig.19A Rear perspective view of the bracket.

[0041] Fig.21 Shows Fig.19A Top view of the bracket.

[0042] Fig. 22 Shows Fig.19A Side view of the bracket.

[0043] Fig.23 Shows Fig.19A Front view of the bracket.

[0044] Fig.24 Shows Fig.19A Rear view of the bracket.

[0045] Fig.25 Shows Fig.19A Front perspective view of the sample tube holder of the rack.

[0046] Fig.26 Shows Fig.25 Rear perspective view of the sample tube holder.

[0047] Fig. 27 Shows Fig.19A A front perspective view of a hinge assembly of a bracket.

[0048] Fig.28 Shows Fig. 27 A rear perspective view of the hinge assembly.

[0049] Fig.29 Shows Fig. 27 Exploded front view of the hinge assembly.

[0050] Figures 30A-30B Shows Fig. 27 Front view of the hinge assembly.

[0051] Fig.31 Shows Fig. 27 Top view of the hinge assembly.

[0052] Fig.32 Shows Fig. 27 A front perspective view of a portion of a hinge assembly.

[0053] Fig.33 Shows Fig. 27 A front perspective view of a portion of a hinge assembly.

[0054] Fig.34 Shows Fig. 27 A top perspective view of a portion of a hinge assembly.

[0055] Fig.35 Shows Fig.19A Top view of the self-locking lugs of the bracket.

[0056] Fig.36 Shows Fig.35 Bottom view of the self-locking lugs.

[0057] Fig.37 Shows Fig.35 A perspective view of the self-locking lugs.

[0058] Fig.38 Shows Fig.35 Side view of the self-locking lug.

[0059] Fig.39 Shows Fig.35 Rear view of the self-locking lugs.

[0060] Figures 40A-40D Shows Fig.19A View of the sample tube holder of the rack.

[0061] Figures 41A-41B Shows Fig.19A View of the sample tube holder of the rack. DETAILED DESCRIPTION

[0062] Described herein is a hinge assembly designed to prevent vertical movement or liftoff of a sample tube from a sample tube holder during a pipetting operation. The hinge assembly can include a hinge support coupled to a movable hinge plate. The hinge support can remain stationary, and in some embodiments, the reagent housing coupled to the support is maintained during the movement of the hinge plate (e.g., the pivoting and sliding movement of the hinge plate relative to the reagent housing of the support). The hinge assembly can include one or more hinge pins, wherein the hinge plate is configured to pivot relative to the hinge support via one or more hinge pins. In some methods of use, a force is applied to the hinge plate to rotate the hinge plate to contact with one or more sample tubes. In some embodiments, the force can be applied by a separator in a receiving chamber of the receiving support. For example, when the support is physically contacted with the feature in the receiving chamber as the support is inserted into the receiving chamber, the force can be applied by a separator.

[0063] The hinge assembly reliably constrains one or more sample tubes in the sample tube holder. A movement caused by the actuation force can cause the hinge assembly to pivot and slide, and thus lock the sample tube in position. Once pivoted and slid into position, the hinge plate can be designed to contact multiple sample tubes simultaneously, thereby fixing each sample tube held by the sample tube holder. Once pivoted and slid, the hinge assembly can prevent the sample tube from experiencing vertical lifting during the pipetting operation, thereby increasing the pipetting efficiency. As further described herein, advantageously, the sample tube is reliably and consistently constrained vertically in a movement by the hinge assembly.

[0064] In certain embodiments, the action of placing the support in the receiving chamber simultaneously pivots and slides the hinge plate and locks the sample tube into position, thereby preventing the movement of the sample tube during the subsequent pipetting operation performed on the support. Before the support is lowered into the receiving chamber, the sample tube can be easily loaded into the sample tube holder. The hinge plate is pivoted and slid to contact with a plurality of sample tubes and constrain a plurality of sample tubes in the sample tube holder. The hinge plate applies a consistent and repeatable force to each sample tube in the sample tube holder. The hinge plate allows easy loading and unloading of sample tubes, thereby minimizing user errors and the application of loading and unloading.

[0065] Described herein is a support for supporting, transporting and transporting reagents and samples for various purposes (particularly in conjunction with sample preparation under clinical background). The support allows the placement and retention of one or more sample tubes. The support allows the placement of one or more corresponding reagent holders. Sample tubes and reagent holders can be positioned to perform a liquid dispensing process associated with sample preparation such as for polynucleotide amplification. The arrangement of the support can minimize cross sample contamination, and allows to perform multiple sample preparations from multiple clinical samples continuously or in parallel.

[0066] In certain embodiments, the sample in the sample tube is obtained from a source, and the source includes an environment or a living source. In certain embodiments, it is suspected that the sample has one or more target analytes (analytes of interest). Biological samples can be obtained from animals (including humans) and include fluids, solids, tissues and gases. Biological samples include urine, saliva and blood products (such as blood plasma, serum, etc.). The sample tube can receive any gas, liquid or solid sample. The sample can be provided as a blood sample, a tissue sample (for example, for example, a swab of nose, oral cavity, anus or vaginal tissue), a needle aspiration type biopsy, a lysate (such as fungi or bacteria). The polynucleotide to be amplified can be contained in microparticles (for example, cells such as white blood cells or red blood cells), tissue fragments, bacteria (for example, gram-positive bacteria or gram-negative bacteria), fungi or spores. One or more liquids (for example, water, buffer, blood, blood plasma, serum, urine, cerebrospinal fluid (CSF) or organic solvent) can be a part of the sample, and / or added to the sample during the processing step. The sample tube can receive any biological or environmental sample. However, such examples should not be construed as limiting the applicable sample types to the present disclosure.

[0067] The support is configured to be insertable into and removable from the diagnostic device. The support is configured to be used for, for example, a diagnostic device that performs automated sample preparation on multiple samples individually or simultaneously. The diagnostic device can perform sample preparation on multiple reagents individually or simultaneously. The support described herein can be used to analyze any sample containing nucleic acid for any purpose, including but not limited to genetic testing and clinical testing for various infectious diseases in humans.

[0068] It should be understood that the embodiments of the systems described herein are not limited to holders that can be inserted into and removed from diagnostic equipment, nor are they limited to holders used to analyze nucleic acids. Embodiments of hinge assemblies according to the present disclosure can be implemented in any suitable holder that receives a sample tube.

[0069] In a non-limiting example, the preparation of the amplification preparation sample can include one or more of the following steps: contacting the neutralized polynucleotide sample with an amplification reagent mixture comprising a polymerase and a plurality of nucleotides and optionally a positive control plasmid and a fluorescent hybridization probe selective for at least a portion of the plasmid; and / or reconstitute the lyophilized particles with a liquid to produce an amplification reagent mixed solution. In certain embodiments, a reagent holder provides all reagents required for preparing the amplification preparation sample. It should be understood that the reagent holder and sample tube described herein are provided by way of example and are not intended to limit the present disclosure. Embodiments of the present disclosure can be implemented using other suitable reagent holders and sample tubes.

[0070] A first exemplary bracket according to the present disclosure

[0071] Figure 1-6 A bracket 100 according to a first embodiment of the present disclosure is illustrated. Figure 1 A front perspective view of the bracket 100 is shown. Figure 2 A rear perspective view of the bracket 100 is shown. Figure 3 A top view of the stent 100 is shown. Figure 4 A side view of the bracket 100 is shown. Figure 5 A front view of the bracket 100 is shown. Figure 6 A rear view of the rack 100 is shown. The rack 100 is configured to receive a plurality of sample tubes 102, and to receive a plurality of reagent holders 104. The rack 100 receives these components in such a manner that the sample tubes 102 and the reagent holders 104 can be loaded separately and independently from each other. In this non-limiting embodiment, the sample tubes 102 are in a one-to-one correspondence with the reagent holders 104. Other configurations are contemplated, such as two sample tubes 102 to one reagent holder 104 or one sample tube 102 to two reagent holders 104.

[0072] Reagent holder 104 all accommodates reagent, to extract polynucleotide from sample, and polynucleotide is placed in amplification preparation form.Reagent holder 104 can be designed for various purposes and keep and transport reagent, including but not limited to sample preparation under clinical background.Reagent holder 104 can comprise treatment tube 174, for various mixing and reaction process that occur during sample preparation.For example, cell lysis can occur in treatment tube 174, as the extraction of nucleic acid (such as DNA or RNA of patient and DNA or RNA of pathogen).Reagent holder 104 can comprise one or more reagent tubes 176, and described one or more reagent tubes 176 can be integrated with reagent holder 104 or removable.Reagent can be in liquid or solid form (such as in freeze-dried form), for performing the extraction of nucleic acid from the sample associated with support 100.Sample preparation can comprise many different pipetting sequences, wherein liquid distributor pipettes material into sample tube 102 and reagent holder 104 and comes out many different times from sample tube 102 and reagent holder 104.

[0073] The rack 100 is configured to receive twelve sample tubes 102 and twelve corresponding reagent holders 104. Although the rack 100 is illustrated with twelve sample tubes 102, the rack 100 is capable of receiving any number of sample tubes 102. Although the rack 100 is illustrated with twelve reagent holders 104, the rack 100 is capable of receiving any number of reagent holders 104. In some embodiments, the rack can receive 1, 2, 4, 6, 8, 10, 12, 16, 20, or 24 sample tubes 102. Each sample can be contained in a separate sample tube 102. In some embodiments, the rack can receive 1, 2, 4, 6, 8, 10, 12, 16, 20, or 24 reagent holders 104. Each reagent holder 102 can contain one or more reagents. Thus, a rack configured to receive twelve sample tubes 102 and twelve corresponding reagent holders 104 may be configured to receive twelve sample tubes 102 and twelve corresponding reagent holders 104. Figure 1 The embodiments of the rack 100 are exemplary. In some embodiments, the rack 100 is configured so that each of the plurality of sample tubes 102 is maintained at the same height relative to each other. In some embodiments, the rack 100 is configured so that each of the plurality of reagent holders 104 is maintained at the same height relative to each other.

[0074] The rack 100 receives a plurality of sample tubes 102 and a plurality of reagent holders 104 within twelve lanes 106, wherein each lane 10 has one sample tube 102 and one reagent holder 104. A lane 106 as used herein in the context of the rack 100 is a dedicated area of ​​the rack 100 designed to receive a sample tube 102 and a corresponding reagent holder 104. Although the rack 100 is illustrated with twelve lanes 106, the rack 100 can include any number of lanes 106.

[0075] The channel 106 includes a first channel and a second channel. The channel 106 is parallel to each other. In certain embodiments, this structure increases the efficiency of pipetting. Usually, when parallel to each other, multiple pairs of adjacent sample channels 106 are separated by 24mm at their respective midpoints. Other distances are possible, such as separated by 18mm or separated by 27mm. The distance between the midpoints can depend on the spacing of the nozzles in the liquid distributor. Keeping a spacing of 9mm realizes easy loading from the support 100 to the 96-well plate, wherein the holes are usually spaced 9mm apart.

[0076] The rack 100 is configured to receive a plurality of sample tubes 102, such as those further described herein. The rack 100 is configured to hold and retain one or more such sample tubes 102. The rack 100 is configured to allow access to samples stored in the sample tubes 102 on a laboratory bench or in a dedicated area of ​​a diagnostic device. The rack 100 is configured to allow access to the sample tubes 102 to be accessed by one or more other functions of the diagnostic device, such as automated pipetting.

[0077] Features of a non-limiting example support that can be implemented according to the present disclosure will now be described in the support 100 illustrated in the figures. It should be understood that other suitable supports can be implemented according to the present disclosure, and the features of the support 100 are not intended to limit the embodiments of the hinge assembly described herein. The support 100 is configured to receive a given sample tube 102 in such a manner that the sample tube 102 is reversibly retained in a suitable position and thereby remains stable when the sample is approached in the sample tube 102. The support 100 is configured to receive a given sample tube 102 in such a manner that the sample tube 102 is retained when the support 100 is being transported from one place to another. The support 100 is configured to retain a given sample tube 102 when the support 100 is inserted into a diagnostic device. The support 100 is configured to receive a given sample tube 102 in such a manner that the sample tube 104 is reversibly retained in a suitable position when the pipette tip enters and leaves the sample tube 104. The rack 100 is configured to receive a given sample tube 102 and prevent vertical movement or lift-off during pipetting operations.Other suitable features and configurations of the rack 100 are possible.

[0078] The support 100 can include two or more sub-components. The support 100 includes a sample tube holder 108 configured to receive one or more sample tubes 102. In this non-limiting example, the support 100 also includes a reagent housing 110 configured to receive one or more reagent holders 104. The sample tube holder 108 and the reagent housing 110 can be coupled to form an integral structure. The sample tube holder 108 and the reagent housing 110 can be reversibly coupled, such as by the use of fasteners, to allow assembly and disassembly of the sample tube holder 108 and the reagent housing 110. It should be understood that according to the present disclosure, the sample tube holder 108 can be coupled to any suitable sub-component, including but not limited to the reagent housing 110.

[0079] The rack 100 can be divided into one or more channels 106. Each channel 106 of the rack 100 includes a first position 112 configured to receive a sample tube 102 and a second position 114 configured to receive a reagent holder 104. The first position 112 is located on the sample tube holder 108. The second position 114 is located on the reagent housing 110. Each channel 106 is configured to process a sample contained in the sample tube 102 according to one or more sample processing steps, including mixing the sample with one or more reagents in a corresponding reagent holder.

[0080] In a non-limiting example, the support 100 includes a feature of locating and retaining a reagent holder 104. In the illustrated embodiment, each of the second position 114 in the corresponding channel 106 includes a mechanical key 116 configured to receive the reagent holder 104 along a single orientation. The reagent holder 104 slides horizontally to the slot 120 in the reagent housing 110. The reagent holder 104 can engage with the reagent housing 110 via the mechanical key 116 that keeps the reagent holder 104 in position. For example, the mechanical key 116 can include a protrusion or a recessed portion that allows the reagent holder 104 to snap into the second position 114 when engaging with the complementary portion of the reagent holder 104. In certain embodiments, the edge of the reagent holder 104 engages with the complementary groove in the upper portion of the slot 120. In certain embodiments, the reagent holder 104 is locked into position in the reagent housing 110 such as by utilizing a locking structure. The locking structure can be identifiable as locking by audible or physical prompts.

[0081] The reagent housing 110 can be configured so that, when positioned in the reagent housing 110, the reagent holder 104 is aligned to be picked up using an appropriate pipette tip of a liquid dispenser. In addition, the second position 114 of each channel 106 can be deep enough to accommodate one or more pipette tips such as those contained in a pipette tip sheath, as described herein. When positioned in the reagent housing 110, the reagent holder 104 is configured to be acted upon by one or more components of a diagnostic device, as described herein.

[0082] The reagent housing 110 can include various features. The reagent housing 110 can have four feet 124, which are used to stabilize the reagent housing 110 and are used as position locators when the support 100 is inserted into the dedicated part of the equipment. The reagent housing 110 can include a handle 126. The handle 126 can be attached to the reagent housing 110 at any position (such as at the midpoint of the reagent housing 110). The part of the reagent housing 110 that receives the reagent holder 104 can be made of any suitable material (including metal or plastic). The reagent housing 110 can be made of a metal (such as aluminum) that is both light and can be processed to high tolerances. The reagent housing 110 can be made of sufficiently strong materials to ensure that the reagent housing 110 remains stable when it is located in the diagnostic device. The reagent housing 110 includes one or more alignment components 130. The alignment component 130 includes four close tolerance pins located at each corner of the reagent housing 110. The registration feature 130 fits tightly into a complementary hole in a receiving area (such as a recessed area) that receives the diagnostic device and thereby stabilizes the reagent housing 110 within the diagnostic device.

[0083] The reagent housing 110 includes a horizontal member 132 and two vertical members 134 connected to the horizontal member 132. Each of the vertical members 134 includes two feet 124, but other configurations of the vertical members 134 and the feet 124 are contemplated. Each of the second positions 114 of each respective channel 106 is a recessed portion within the horizontal member 132. The two vertical members 134 are configured to allow the reagent housing 110 to stand or maintain stably. When positioned, the feet 124 symmetrically attached to the first and second vertical members 134 provide additional stability for the reagent housing 110.

[0084] The rack 100 includes features for positioning and retaining sample tubes 102. The rack 100 is configured to receive a plurality of samples, each sample being received in an individual sample tube 102. The samples received in the sample tubes 102 may be from a common source or from different sources. As described above, each sample tube 102 is mounted in a single channel 106 of the rack 100 adjacent to a corresponding reagent holder 104.

[0085] Figure 7 and 8 A non-limiting example sample tube holder 108 according to the present disclosure is illustrated. Figure 7 A front perspective view of the sample tube holder 108 is shown, and Figure 8 A rear perspective view of the sample tube holder 108 is shown. Figure 7 and 8 A non-limiting example of a sample tube holder 108 is described that includes a first portion 142, a second portion 146, a third portion 154, and a fourth portion 160. However, it should be understood that any suitable shape and form of sample tube holder 108 can be implemented in embodiments of the present disclosure and need not include all or any of the first, second, third, and fourth portions included in the illustrated embodiment.

[0086] The sample tube holder 108 includes a first portion 142. The first portion 142 is flat or substantially flat. The first portion 142 is horizontal or substantially horizontal. The first portion 142 includes a plurality of upper openings 144. The first portion 142 is configured to receive twelve sample tubes 102 through twelve upper openings 144, respectively. Although the sample tube holder 108 is illustrated with twelve upper openings 144, the sample tube holder 108 can include any number of upper openings 144. The upper openings 144 can be rounded or circular, but can have other cross-sectional shapes, depending on the characteristics of the sample tubes 102. The number of upper openings 144 can correspond to the number of sample tubes 102 in a one-to-one correspondence. The first portion 142 can include one or more rounded edges, such as rounded corners or bends, to connect the first portion 142 to another portion of the sample tube holder 108.

[0087] The sample tube holder 108 includes a second portion 146. The second portion 146 is flat or substantially flat. The second portion 146 is vertical or substantially vertical. The second portion 146 can extend along a portion of the length of the sample tube 102, such as at least 30% of the length, at least 40% of the length, at least 50% of the length, at least 60% of the length, at least 70% of the length, or any range of the above values. The second portion 146 includes an upper post 148. The upper post 148 can extend vertically upward from the first portion 142. Each upper post 148 has an opening 150 configured to receive a fastener therethrough. Any segment of the second portion 146 can include one or more openings 150.

[0088] The sample tube holder 108 is configured to be coupled to the reagent housing 110 such that the two components of the rack 100 are rigidly connected. The opening 150 of the sample tube holder 108 is configured to align with a corresponding opening in the reagent housing 110. Any suitable fastener (such as, but not limited to, a screw or a pin) can extend through the opening 150 of the sample tube holder 108 and the corresponding opening in the reagent housing 110 to couple the sample tube holder 108 to the reagent housing 110. Although two openings 150 are illustrated, the sample tube holder 108 can include any number of openings 150.

[0089] The second portion 146 can include one or more rails 152. The rails 152 extend horizontally from the sides of the second portion 146. The rails 152 extend the length of the second portion 146 or a portion thereof. The rails 152 extend in the same direction as the first portion 142.

[0090] The sample tube holder 108 of this non-limiting example includes a third portion 154. It should be understood that other example sample tube holders 108 according to the present disclosure may not include the third portion 154, and may include only some or all of the first portion 142, the second portion 146, the fourth portion 160, or any combination of these portions arranged to retain the sample tube 102. In this example, the third portion 154 is oriented at the diagonal between the second portion 146 and the fourth portion 160. The third portion 154 extends in the same direction as the first portion 142. The first portion 142 and the third portion 154 are skewed. The third portion 154 includes a plurality of lower openings 156. The third portion 154 is configured to receive twelve sample tubes 102 through twelve lower openings 156, respectively. Although the sample tube holder 108 is illustrated with twelve lower openings 156, the sample tube holder 108 can include any number of lower openings 156. The number of lower openings 156 can correspond to the number of sample tubes 102 in a one-to-one correspondence. The lower opening 156 can have any suitable cross-sectional shape, including but not limited to an elliptical elongated opening, an oval opening, and a circular opening. The lower opening 156 is shaped and configured to accommodate the circumference of the sample tube 102. The third portion 154 can include one or more rounded edges, such as rounded corners or bends, to connect the third portion 154 to another portion of the sample tube holder 108.

[0091] The sample tube holder 108 includes a fourth portion 160. One or more portions of the fourth portion 160 can be flat or substantially flat. One or more portions of the fourth portion 160 can be horizontal or substantially horizontal. Portions of the first portion 142 and the fourth portion 160 can be parallel. The fourth portion 160 can form a base of the sample tube holder 108. The fourth portion 160 can include one or more rails 164. The rails 164 can extend vertically from the sides of the fourth portion 160. Although three rails 164 are illustrated, the fourth portion 160 can include any number of rails 164.

[0092] In a method of use according to the present disclosure, each of a plurality of sample tubes 102 is inserted into the sample tube holder 108. The distal end 166 of the sample tube 102 is first inserted into the upper opening 144 of the first portion 142. The distal end 166 of the sample tube 102 then passes vertically through, parallel to the second portion 146. The distal end 166 of the sample tube 102 is next inserted into the lower opening 156 of the third portion 154. The distal end 166 of the sample tube 102 then passes vertically through to rest on the surface of the fourth portion 160. The proximal end 170 of the sample tube 102 extends above the first portion 142 of the sample tube holder 108 when the sample tube 102 is received therein.

[0093] The proximal end 170 of the sample tube 102 can include a cap 172. The cap 172 can be a removable cap attached to the proximal end 170. The cap 172 can include a pierceable seal configured to be pierced by a pipette tip. The pierceable seal can include a metal foil, a plastic layer, an elastomeric film, or any combination of these or other suitable seal structures. The cap 172 can be configured to allow access to the sample retained in the sample tube 102 through the proximal end 170. In this non-limiting embodiment, the cap 172 is configured to be penetrated by the pipette tip to allow access to the sample in the sample tube 102 via a liquid dispenser. The cap 172 extends above the first portion 142. In some instances, the cap 172 can abut the first portion 142. The cap 172 can have a diameter larger than the diameter of the upper opening 144. It should be understood that embodiments of the present disclosure do not require the sample tube 102 to have a cap 172 attached to its proximal end 170. A sample tube 102 having an open (eg, uncapped) proximal end can be received and retained in a sample tube holder described herein consistent with the present disclosure.

[0094] In the illustrated embodiment, the sample tube 102 is held near the proximal end 170 by the upper opening 144. The upper opening 144 can contact at least one portion of the sample tube 102. The upper opening 144 can contact two opposing portions of the sample tube 102. The upper opening 144 can contact the circumference of the sample tube 102 or a portion thereof. The rounded shape of the upper opening 144 can match the periphery of the sample tube 102. The sample tube 102 is held near the distal end 166 by the lower opening 156. The lower opening 156 can contact at least one portion of the sample tube 102. The lower opening 156 can contact two opposing portions of the sample tube 102. The lower opening 156 can contact the circumference of the sample tube 102 or a portion thereof. Each of the upper opening 144 and the lower opening 156 can be a ring or an open ring. Each of the upper opening 144 and the lower opening 156 can form a hole in the sample tube holder 108. The sample tube holder 108 can be constructed of any suitable material, such as a metal.

[0095] The sample tube 102 can be held at its top and bottom by the sample tube holder 108. The sample tube holder 108 can prevent or limit movement in the horizontal direction. The upper opening 144 and the lower opening 156 can limit movement in the horizontal direction based on the shape of the openings 144, 156 similar to the outer surface of the sample tube 102. The sample tube holder 108 can allow movement of the sample tube 102 in the vertical direction. The sample tube 102 can be freely inserted into the sample tube holder 108 or removed from the sample tube holder 108. The sample tube 102 is moved vertically or substantially vertically to insert the sample tube 102 into the sample tube holder 108. The sample tube 102 is moved vertically or substantially vertically to remove the sample tube 102 from the sample tube holder 108. This method can be repeated to vertically insert a plurality of sample tubes 102 into the sample tube holder 108.

[0096] Advantageously, when disposed within the sample tube holder 108, embodiments of the present disclosure allow portions of the sample tube 102 to be accessible to a sample identifier verifier, such as a barcode reader. The front surface of each sample tube 102 can be identified by a scanner for a patient or sample, as described herein. The sample tube 102 can be rotated by a user within the sample tube holder 108 so that the sample identifier faces outward. The sample tube 102 can include an identifier surrounding the sample tube 102.

[0097] Example hinge assembly according to a first embodiment of the present disclosure

[0098] An exemplary hinge assembly 200 according to a first embodiment of the present disclosure will now be referred to. Figure 9-14 It should be understood that the hinge assembly of the present disclosure is not limited to the features of the example hinge assembly 200 and can take other forms, shapes and sizes consistent with the present disclosure. Fig. 9FIG. 10 shows a front perspective view of the hinge assembly 200. FIG. 11 shows a side view of the hinge assembly 200. FIG. Fig.12 A top view of hinge assembly 200 is shown. Fig.13 An exploded front perspective view of a portion of hinge assembly 200 is shown. Fig.14 An exploded front perspective view of the hinge assembly 200 is shown.

[0099] The hinge assembly 200 includes a hinge plate 202. The hinge assembly 200 includes a hinge support 204. The hinge assembly 200 includes one or more hinge pins 206. Fig.13 and 14 The hinge support 204 and hinge pin 206 are illustrated in exploded view. The hinge support 204 can be an elongated member. The hinge support 204 has one or more openings 210 configured to receive a fastener therethrough. Any segment of the hinge support 204 can include one or more openings 210. The openings 210 are configured to align with corresponding openings in the reagent housing 110. When the fasteners extend through the corresponding openings, the hinge support 204 of the hinge assembly 200 is rigidly coupled to the reagent housing 110. Although three openings 210 are illustrated, the hinge support 204 can include any number of openings 210.

[0100] The hinge support 204 can include one or more hubs 212, such as in Fig.13 The hub 212 is located near the end of the hinge support 204 along the longitudinal axis L of the hinge support. HS In the illustrated non-limiting embodiment, each hub 212 has a hollow cylindrical section extending inwardly from the end of the hinge support 204 within the opening 214. It should be understood that the present disclosure is not limited to the hub 212, and other shapes and sizes are possible. The opening 214 can be along the longitudinal axis L of the hinge support 204. HS Each opening 214 is configured to receive a hinge pin 206. The hub 212 can be a toggle or hollow portion of a hinge joint that creates the hinge plate 202 to rotate about. The hub 212 can be the location in which the hinge pin 206 is disposed. The hub 212 can be configured as a cylinder or hemispherical shape. Other configurations are possible.

[0101] The hinge support 204 can include a rear surface 216, as illustrated in Figure 10. The rear surface 216 can be flat to abut a flat surface of the reagent housing 110. The rear surface 216 can be any shape that allows for a secure connection between the hinge support 204 and the reagent housing 110 when fastened together.

[0102] The hinge pin 206 can be a generally cylindrical member, and the corresponding opening 214 can be correspondingly cylindrical. It should be understood that any appropriately shaped and sized mating configuration can be implemented in embodiments of the present disclosure. The hinge pin 206 can provide a pivot axis about which the hinge assembly 200 can pivot. In other words, the pivot or articulation axis of the hinge assembly 200 can be a longitudinal axis L along the hinge pin 206. HP During the pivoting motion, the hinge support 204 is configured to remain stationary. In some cases, the hinge pin 206 also remains stationary. The hinge plate 202 is configured to pivot or rotate relative to the fixed hinge support 204. The hinge pin 206 provides an axis of rotation in which all other parallel and rotational movements are prevented or restricted. The hinge assembly 200 can have one degree of freedom of motion. For example, as in Fig.11A As shown in FIG. 2 , the hinge plate 202 of the hinge assembly 200 can rotate in one dimension in a clockwise or counterclockwise direction.

[0103] Fig.11A A first side view of the hinge plate 202 is illustrated. Fig. 11B A second side view of the hinge plate 202 is shown. The features of the hinge plate 202 will now be referred to. Fig.11A The hinge plate 202 includes a first portion 220, a second portion 224, and a third portion 234. The first portion 220 can be flat or substantially flat. In this non-limiting embodiment, when the third portion 234 is positioned parallel to the axis X, the first portion 220 is bent from the horizontal by an angle α, such as in Fig.11A . The angle α relative to the axis X can be 0° from horizontal so that the first portion 220 is horizontal, 1° from horizontal, 2° from horizontal, 3° from horizontal, 4° from horizontal, 5° from horizontal, 6° from horizontal, 7° from horizontal, 8° from horizontal, 9° from horizontal, 10° from horizontal, or any range of the above values. It should be understood that other configurations are possible. Back to reference Fig. 10A , the first portion 220 includes a plurality of notches 222. The first portion 220 is configured to retain twelve sample tubes 102 using twelve notches 222, respectively. Although the hinge plate 202 is illustrated with twelve notches 222, the hinge plate 202 can include any number of notches 222. The notches 222 can be rounded. The notches 222 can be circular. The notches 222 can be semicircular or hemispherical. The notches 222 can be tapered. The number of notches 222 can correspond to the number of upper openings 144 in the sample tube holder 108 in a one-to-one correspondence. The first portion 220 can include one or more rounded edges, such as rounded corners or bends, to connect the first portion 220 to another portion of the hinge plate 202.

[0104] The hinge plate 202 includes a second portion 224. The second portion 224 is flat or substantially flat. In this non-limiting embodiment, when the third portion 234 is positioned perpendicular to the axis Z, the second portion 224 is bent from the vertical by beta (β), as in Fig.11A . The angle β relative to the axis Z can be from vertical 0°, so that the second portion 224 is vertical, from vertical 2°, from vertical 4°, from vertical 6°, from vertical 8°, from vertical 10°, from vertical 12°, from vertical 14°, from vertical 16°, from vertical 18°, from vertical 20°, or any range of the above values. It should be understood that other configurations are possible. When the support 100 is assembled, the second portion 224 can extend along a portion of the length of the sample tube 102, such as at least 5% of the length, at least 10% of the length, at least 15% of the length, at least 20% of the length, at least 25% of the length, or any range of the above values. When the support 100 is assembled, the second portion 146 can extend at least along the length of the cap 172 (if present on the sample tube).

[0105] The second part 224 has one or more openings 226 configured to receive fasteners or tools therethrough. Any section of the second part 224 can include one or more openings 226. The opening 226 is configured to align with the corresponding opening 210 in the hinge support 204. The opening 226 is configured to align with the corresponding opening in the reagent housing 110. The opening 226 allows a tool (such as but not limited to a screwdriver) to extend through the opening 226. The tool can install fasteners by the opening 210 in the hinge support 204 and the opening in the reagent housing 110 to rigidly couple the hinge support 204 and the reagent housing 110. Although three openings 226 are illustrated, the second part 224 can include any number of openings. The opening 226 can be configured to provide an entrance to the opening 210 in the hinge support 204, so that the hinge support 204 is attached to the reagent housing 110.

[0106] The second portion 224 includes a flange 230. The flange 230 is generally formed by Fig.11A 20. The hub 230 extends in a plane defined by the X and Z axes illustrated in FIG. The hub 230 extends horizontally near the edge of the second portion 224. The hub 230 extends in a direction opposite to the first portion 220. Each flange 230 includes an opening 232 extending through the flange 230. The openings 232 can be coaxial. Each opening 232 is configured to receive a hinge pin 206. The pair of flanges 230 are spaced apart to receive the hinge support 204 therebetween. The space between the pair of flanges 230 can be slightly longer than the length of the hinge support 204.

[0107] The hinge plate 202 includes a third portion 234. The third portion 234 can be flat or substantially flat. In this non-limiting embodiment, when the third portion 234 is positioned parallel to the axis X, the first portion 220 is bent from the horizontal by an angle α, such as in Fig.11A . In other embodiments, the third portion 234 may not be parallel to the axis X and may be bent relative to the axis X, depending on the shape and size of the sample tube holder 108 and the rack 100 with which the hinge assembly 200 interfaces. In one non-limiting embodiment, the first portion 220 and the third portion 234 can be parallel or substantially parallel. The third portion 234 can form the base of the hinge plate 202.

[0108] Example method of operating the hinge assembly of the first embodiment of the present disclosure

[0109] Figures 15A-15C Views illustrating the operation of the hinge assembly 200 according to the first embodiment of the present disclosure. Fig.15A is a side view of a sample tube 102 being inserted into a sample tube holder 108 . Fig. 15B is a side view of the sample tube 102 fully inserted into the sample tube holder 108. Fig. 15C 1 is a top view of the sample tube 102 and the sample tube holder 108 when the sample tube 102 is fully inserted. Figures 15A-15C In a first configuration, illustrated in FIG. 1 and referred to herein as a "tube insertion configuration," the hinge assembly 200 is configured to allow a sample tube 102 to be inserted into a sample tube holder 108. The following description describes the hinge assembly 200 in the tube insertion configuration.

[0110] In the tube insertion configuration, the first portion 220 of the hinge plate 202 is positioned closer to the reagent housing 110. The first portion 220 of the hinge plate 202 is positioned further away from the upper opening 144 of the first portion 142 of the sample tube holder 108. The first portion 220 of the hinge plate 202 is rotated away from the upper opening 144 of the sample tube holder 108 (rotated toward the reagent housing 110) to provide clearance for the sample tube 102 to be inserted into the upper opening 144 of the sample tube holder 108. For example, in the tube insertion configuration, the first portion 220 of the hinge plate 202 is positioned to provide clearance for the sample tube 102 to be vertically inserted into the sample tube holder 108.

[0111] In the tube insertion configuration, the third portion 234 of the hinge plate 202 is rotated away from the bottom of the reagent housing 110. The third portion 234 of the hinge plate 202 is rotated to Figures 15A-15CThe construction illustrated in provides a gap for the sample tube 102 to be inserted. In this construction, a portion of the third portion 234 of the hinge plate 202 can extend below the first portion 142 of the sample tube holder 108. In some cases, the third portion 234 of the hinge plate 202 can be positioned at an angle γ relative to the first portion 142 of the sample tube holder 108. The angle γ can be an acute angle. The angle γ can be 2° from horizontal, 4° from horizontal, 6° from horizontal, 8° from horizontal, 10° from horizontal, 12° from horizontal, 14° from horizontal, 16° from horizontal, 18° from horizontal, 20° from horizontal, or any range of the above values. The third portion 234 of the hinge plate 202 can abut the first portion 142 of the sample tube holder 108 at position P. The contact between the third portion 234 of the hinge plate 202 and the first portion 142 of the sample tube holder 108 can limit further rotation of the hinge plate 202 in the clockwise direction, such as from Fig.15A and 15B Observed from the perspective of.

[0112] Fig. 15C A top view of the tube insertion configuration is shown. Fig.15A and 15B , the first portion 220 of the hinge plate 202 is rotated toward the reagent housing 110 (away from the first portion 142 of the sample tube holder 108). The notch 222 of the first portion 220 of the hinge plate 202 provides clearance for inserting the sample tube 102 into the sample tube holder 108 along the vertical axis A of the upper opening 144 in the first portion 142. Fig. 15C , the notch 222 is laterally offset a distance d from the upper opening 144 of the sample tube holder 108 when viewed from the top of the hinge assembly 200. The lateral offset distance d can be a distance that allows a user to insert a sample tube 102 into the sample tube holder 108 without interference from or contact with the hinge assembly 200.

[0113] The operation of the hinge assembly 200 according to the present disclosure includes moving the hinge plate 202 from Figures 15A-15C The tube insertion structure shown in the figure moves to Fig.16A and 16B A second configuration is illustrated in and referred to herein as a “tube retaining configuration.” Fig.16A is a side view of the hinge assembly 200 after actuation. Fig. 16Bis a top view of a sample tube 102 and a sample tube holder 108 after the hinge assembly 200 is actuated. As described herein, the hinge plate 202 is capable of pivoting relative to the hinge support 204. During the pivoting motion, the hinge support 204 is configured to remain stationary, and the hinge plate 202 pivots relative to the stationary hinge support 204. The hinge assembly 200 is configured to allow the sample tube 102 to be retained in a vertical orientation. The following description describes the hinge assembly 200 in a tube retaining configuration.

[0114] In this non-limiting embodiment, in the tube retaining configuration, the first portion 220 of the hinge plate 202 is positioned in a horizontal or substantially horizontal orientation, such as in Fig.16A In one non-limiting embodiment, from Fig.16A , the first portion 220 of the hinge plate 202 can be rotated counterclockwise. In one non-limiting embodiment, the first portion 220 of the hinge plate 202 can be rotated past horizontal. In these example embodiments, contact between the hinge plate 202 and the sample tube 102 (or cap 172, if applicable) limits further counterclockwise rotation of the hinge plate 202. The first portion 220 of the hinge plate 202 retains the sample tube 102 within the sample tube holder 108. Fig. 15B In contrast, the first portion 220 of the hinge plate 202 is pivoted to contact a portion of the sample tube 102 or the cap 172 of the sample tube 102. The first portion 220 of the hinge plate 202 rests on a top surface of the cap 172 of the sample tube 102.

[0115] The notch 222 of the first portion 220 of the hinge plate 202 can be semicircular or hemispherical to allow access to the contents of the sample tube 102. In the tube retention configuration, the notch 222 of the first portion 220 of the hinge plate 202 does not interfere with the center area or region C of the top of the sample tube 102. The notch 222 of the first portion 220 of the hinge plate 202 can be semicircular or hemispherical to cover a portion of the cap 172 but not the center area C. The notch 222 can retain multiple sample tubes 102 in the sample tube holder 108 at the same time. Multiple portions of the first portion 220, including the portion surrounding the notch 222, can collectively and simultaneously apply a vertical retention force to all sample tubes positioned in the sample tube holder 108.

[0116] The second portion 224 of the hinge plate 202 can form an angle delta (δ) relative to the vertical axis A of the upper opening 144. The angle δ can be 0° from vertical, 2° from vertical, 4° from vertical, 6° from vertical, 8° from vertical, 10° from vertical, 12° from vertical, 14° from vertical, 16° from vertical, 18° from horizontal, 20° from vertical, or any range of the above values. Other configurations are possible. Fig. 15B Compared to the position in , the second portion 224 of the hinge plate 202 is pivoted in a counterclockwise direction.

[0117] When the hinge assembly 200 is in the tube retaining configuration, the third portion 234 can be horizontal or substantially horizontal. When the hinge assembly 200 is in the tube retaining configuration, the third portion 234 of the hinge plate 202 can be parallel to the horizontal member 132 of the reagent housing 110. When the hinge assembly 200 is in the tube retaining configuration, the third portion 234 of the hinge plate 202 can be parallel to the first portion 142 of the sample tube holder 108.

[0118] In embodiments of the present disclosure, an actuation force causes the hinge plate 202 to pivot to move the hinge assembly 200 from a tube insertion configuration to a tube retention configuration. In some embodiments, the same actuation force causes the hinge plate 202 to pivot to move the hinge assembly 200 from the tube retention configuration to the tube insertion configuration. In other embodiments, a second, different actuation force is capable of moving the hinge assembly 200 from the tube retention configuration to the tube insertion configuration. In one example, a user applies an actuation force to a portion of the hinge assembly 200 to rotate the hinge assembly 200 between configurations. For example, a user is capable of applying a downward force on the first portion 220 to move the hinge assembly 200 from the tube insertion configuration to the tube retention configuration. Similarly, a user is capable of applying an upward force on the first portion 220 to move the hinge assembly 200 from the tube retention configuration to the tube insertion configuration. In this embodiment, the weight of the first portion 220 of the hinge plate 202 is capable of providing a downward force to prevent vertical movement of the sample tube 102. Fig.16A In another example illustrated in FIG. 1 and described in detail below, the structural features of the receiving compartment in which the support 100 is received exert an actuation force on the third portion 234 to move the hinge assembly 200 from the tube insertion configuration to the tube retention configuration. The structural features are capable of maintaining the actuation force, thereby locking the hinge plate 202 against the sample tube 102.

[0119] It should be understood that any of the first portion 220, the second portion 224, and the third portion 234 of the hinge plate 202 can be contacted by the actuation force to pivot the hinge assembly 200. For example, the first portion 220 can be pushed downward, the segment portion 224 can be pushed horizontally, and / or the third portion 234 of the hinge plate 202 can be pushed upward to move the hinge plate 202 into engagement with the sample tube 102. Fig. 15B In contrast, the third portion 234 of the hinge plate 202 has been actuated to change the position of the hinge assembly 200. Fig.15A and 16A The third portion 234 of the hinge plate 202 is actuated to rotate the hinge assembly 200 in a counterclockwise direction from a perspective of . The third portion 234 of the hinge plate 202 can be a lever arm that allows the hinge plate 202 to pivot.

[0120] Embodiments of hinge assembly 200 can be actuated by a divider 250 in a receiving compartment of receiving support 100 to move from a tube insertion configuration to a tube retention configuration. Divider 250 can include a surface, wall, shelf-like protrusion, enclosure, or any other suitable structure that is shaped, sized, and positioned in the receiving compartment to actuate hinge assembly 200 when support 100 is inserted into the receiving compartment. For example, in a non-limiting example, divider 250 can include one or more pins, rods, or bars positioned in the receiving compartment to interact with hinge assembly 200 when support 100 is inserted into the receiving compartment. Divider 250 is shaped and sized to separate the receiving compartment into two regions, a first region and a second region. When support 100 is received in the receiving compartment, reagent housing 110 is positioned in the first region, and sample tube holder 108 is positioned in the second region. When support 100 is located in the receiving compartment, divider 250 is located between reagent housing 110 and sample tube holder 108.

[0121] When the bracket 100 is inserted into the receiving compartment, the partition 250 acts on the hinge assembly 200. Fig.15A The tube insertion configuration shown in FIG. is transformed into Fig.16A 2, the spacer 250 contacts the third portion 234 of the hinge plate 202. The spacer 250 applies a counterclockwise force on the third portion 234 of the hinge plate 202. The interaction of the spacer 250 with the third portion 234 thus causes the hinge plate 202 to rotate about the hinge support 204 and causes the hinge assembly 200 to transition from the tube insertion configuration to the tube retention configuration.

[0122] The hinge assembly 200 has advantages over other alternative systems designed to retain a sample tube 102 in a sample tube holder 108 during a pipetting operation. One alternative system uses a row of spring clips to apply a horizontal force to each cap 172 of a sample tube 102 positioned in the sample tube holder 108. The spring clips can be arranged in a horizontal row between the upper stems 148 of the second portion 146 of the sample tube holder 108. The arms of the spring clips contact and apply a horizontal force to one side of the cap 172 of the sample tube 102 positioned in the sample tube holder 108. This force and the reaction force applied to the body of the sample tube 102 by the upper opening 144 of the sample tube holder 108 can prevent the sample tube 102 from moving out of the sample tube holder 108 in a horizontal direction. The distal end of the sample tube 102 can contact the fourth portion 160 of the sample tube holder 108. The force applied by the spring clip, the force applied by the upper opening 144, and the friction in the contact area at the bottom of the sample tube 102 can prevent the sample tube 102 from being lifted out of the sample tube holder 108 in a vertical direction.

[0123] In some cases, differences in the sizes of the sample tubes 102 and the caps 172 can prevent the system of spring clamps from consistently preventing the sample tubes 102 from moving in a vertical direction. In some cases, differences in the spring forces generated by the arms of the spring clamps (due to manufacturing differences, material differences, or wear of the spring clamps over time) can prevent the system of spring clamps from consistently preventing the sample tubes 102 from moving in a vertical direction. In such cases, one or more sample tubes 102 can be lifted vertically out of the sample tube holder 108 when the pipette tip is vertically moved upward out of the cap 172 and a vertical force sufficient to vertically displace the sample tubes 102 is applied to the sample tubes 102.

[0124] Therefore, in some previous designs, the sample tube 102 can be lifted during the liquid dispensing operation. In some cases, the sample tube 102 can be retained on the pipette tip of the liquid dispenser, for example due to the friction between the pipette tip and the cap 172. The spring clip can inconsistently retain the sample tube 102 in the sample tube holder 108, and in some cases, up to 25% of the sample tubes 102 can experience a certain degree of vertical lifting through the liquid dispenser. The vertical lifting of the sample tube 102 to any extent can interfere with the pipetting operation and cause the liquid dispenser to malfunction or operate at less than optimal speed and accuracy. In some extreme cases, the pipette tip cannot be separated from the sample tube 102 that is not fully constrained in the sample tube holder 108, potentially causing system interruption and failure. In order to ensure reliability and maximum throughput, it is hoped that the number of sample tubes 102 that experience vertical lifting is zero or close to zero.

[0125] Advantageously, embodiments of the present disclosure reliably constrain sample tubes 102 in sample tube holders 108, and can reduce the number of tubes that undergo vertical lifting to zero. Embodiments of the support 100 including hinge assemblies 200 according to the present disclosure reliably retain sample tubes 102 during instrument workflows. The upper opening 144 of the sample tube holder 108 can prevent the sample tube 102 from moving in a horizontal direction. In some cases, the force applied to the sample tube 102 by the hinge assembly 200 can also prevent the sample tube 102 from moving in a horizontal direction. The fourth portion 160 of the sample tube holder 108 and the hinge assembly 200 together prevent the sample tube 102 from moving in a vertical direction. The partition 250 can lock the hinge plate 202 in a tube retention configuration, thereby locking the sample tube 102 in a proper position and preventing the vertical lifting of the sample tube 102. Advantageously, the shape, size, and position of the hinge assembly 200 can be adapted to adjust the maximum retention force required to retain the sample tube 102.

[0126] Advantageously, embodiments of the present disclosure are capable of reliably and consistently locking sample tubes in one motion. When the divider contacts the hinge plate 202, the hinge plate 202 of the hinge assembly is pivoted by the actuation force of the divider 250. In some embodiments, the descent of the support 100 relative to the divider 250 is a motion that pivots the hinge plate 202 and thereby locks the sample tube 102. The divider 250 applies an upward force to the hinge plate 202, thereby pivoting the hinge plate 202 into a suitable position. Before the support 100 is lowered, the hinge plate 202 can be freely pivoted so that the sample tube 102 can be easily loaded into the sample tube holder 108. The hinge plate 202 is in consistent contact with multiple sample tubes 102, so that the hinge plate 202 can constrain all sample tubes 102 in the sample tube holder 108 at the same time. The overall structure of the hinge plate 202 allows a consistent force ratio to be applied to each sample tube 201. Furthermore, the pivoting motion of the hinge plate 202 is repeatable, such that the same actuation force results in the same pivoting motion of the hinge plate. Advantageously, the hinge plate is easily moved between configurations to easily load and unload batches of sample tubes during a series of diagnostic tests performed consecutively using the same rack, thereby minimizing user errors and the time to load and unload racks, and increasing pipetting efficiency.

[0127] In some embodiments, the hinge assembly 200 contacts the sample tube 102, such as contacting the cap 172 of the sample tube 102. In some embodiments, the hinge assembly 200 applies a downward force on the sample tube 102. In some embodiments, the hinge assembly 200 in the tube retention configuration prevents all vertical movement of the sample tube 102 in the sample tube holder 108. In some embodiments, the hinge assembly 200 in the tube retention configuration allows a certain vertical movement of the sample tube 102 in the sample tube holder 108. In some embodiments, one or more sample tubes can be lifted vertically a small distance during a pipetting operation, but the presence of the hinge plate 202 prevents the sample tube from moving vertically to the extent that the performance of the pipetting system is affected. In some embodiments, the hinge assembly 200 applies a compressive force on the sample tube 102. In some embodiments, the hinge plate 202 of the hinge assembly 200 is positioned laterally above a portion of the sample tube 102 and covers a portion of the sample tube 102 in some cases to prevent upward movement. In some embodiments, the hinge assembly 200 is positioned laterally over and in some cases a portion of the circumference of the cap 172 of the sample tube 102 without obstructing area C of the cap 172 from the pipette tip.

[0128] Advantageously, the hinge plate 202 according to the present disclosure can prevent or limit vertical movement of the sample tube 102 within the sample tube holder 108. In one example, the hinge plate 202 prevents or limits vertical lift-off of the sample tube 102 when the contents of the sample tube 102 are accessed by a liquid dispenser. In another example, the hinge plate 202 prevents or limits vertical movement of the sample tube 102 within the sample tube holder 108 during a fluid handling operation. In yet another example, the hinge plate 202 prevents or limits vertical lift-off of the sample tube 102 by a pipette tip.

[0129] Hinge assembly 200 can include several advantages. Hinge assembly 200 can be easy and intuitive to use. Hinge assembly 200 can be automatically actuated, such as by the simple action of inserting support 100 into the diagnostic device, as described herein. Using hinge assembly 200 can be a simple self-learning process. Hinge support 204 can span reagent housing 110 or a part thereof along the front surface of reagent housing 110. The first portion 220 of hinge plate 202 can be considered as a locking member. Hinge mechanism 200 can be made of any suitable material, including sheet metal.

[0130] Advantageously, embodiments of the present disclosure reliably and consistently unlock sample tubes in one action. The hinge plate 202 of the hinge assembly 200 can be held in place by the divider 250 of the receiving compartment of the receiving rack 100. The rack 100 can be lifted relative to the divider 250 to relieve the force of the divider 250. The hinge plate 202 is thus free to pivot relative to the hinge support 204. One action of raising the rack 100 relative to the receiving compartment can unlock the sample tube 102. After raising the rack 100, the hinge plate 202 can be freely pivoted so that the first sample tube 102 can be easily unloaded from the sample tube holder 108 and the next second sample tube 102 can be loaded. The hinge plate can be pivoted by the user from the tube retention configuration to the tube insertion configuration. The hinge plate 202 can be moved away from the sample tube holder 108 to easily load the next sample tube.

[0131] Hinge assembly 200 does not interfere with the loading and unloading operation of pipe. Advantageously, hinge assembly 200 allows the user to easily load and unload sample tube 102 into support 100. When support 100 is placed in diagnostic equipment, hinge assembly 200 allows the user to lock sample tube 102 in place. Hinge assembly 200 is a locking structure that retains sample tube 102 in support 100 once support 100 is received in receiving compartment. Hinge assembly 200 can facilitate the complete retention of sample tube 102 in sample tube holder 108. When support 100 is placed in diagnostic equipment, hinge assembly 200 can lock sample tube 102 in place. Hinge assembly 200 can serve as a cover above each sample tube 102. In the illustrated embodiment, all sample tubes 102 in sample tube holder 108 are constrained under a physical part (hinge plate 202).

[0132] Embodiments of hinge assemblies according to the present disclosure include additional advantages. Hinge assemblies of the present disclosure can be used with any design of sample tube 102. Hinge assemblies of the present disclosure can also be used with any design of cap 172. Hinge assemblies can be backward compatible so that a support without a hinge assembly can be advantageously improved to include a hinge assembly. As a non-limiting example, a support with a system of spring clips can be improved. In some embodiments, a system of spring clips is moved without separating a sample tube holder from a reagent housing. In some embodiments, a hinge assembly is installed without separating a sample tube holder from a reagent housing.

[0133] Hinge assembly 200 can be directly included in support 100, such as by fixing fasteners, as described herein. Hinge assembly 200 can be easily installed between sample tube holder 108 and reagent housing 110. Sample tube holder 108, hinge support 204 and reagent housing 110 can form an integral structure that is inserted into a receiving compartment in a fluid motion and removed from the receiving compartment in a fluid motion. Hinge plate 202 is designed to pivot relative to the integral structure. Hinge assembly 200 can be easily introduced into a manufacturing supply chain, or can be installed by a user at the moment of use of the support.

[0134] Fig.17 An example diagnostic device 300 according to the present disclosure is shown. The holder 100 can be designed so that it can be easily inserted into and removed from the diagnostic device 300. The reagent housing 110 can include a convenient Figure 2 and 3 One or more alignment members 130 for positioning the support 100 illustrated in the figure. The alignment member 130 is configured to ensure that the support 100 can only be placed in the diagnostic device 300 along a single orientation. In addition, the alignment member 130 can ensure that the support 100 is inserted along the appropriate orientation actuated by the separator 250. It is hoped that the support 100 is correctly positioned in the diagnostic device 300 when the movement is limited thereafter, so that the movement of the liquid dispenser will not be impaired during the liquid handling operation. For example, the alignment member 130 can limit movement in two directions, such as lateral movement and forward and backward movement of the support 100. Movement in the vertical direction can be limited based on the weight of the support 100, wherein the user can lift the support 100 from the diagnostic device 300. When placed in the diagnostic device 300, the alignment member 130 can provide stability for the support 100.

[0135] In certain embodiments, support 100 or diagnostic equipment 300 can include a sensor configured to indicate the proper placement of support 100 in diagnostic equipment 300. The sensor can communicate with the processor to provide a warning to the user, such as an auditory warning or a visual warning via an interface communication, if support 100 is not properly placed. The sensor can be configured to prevent the sample preparation process from starting or continuing if the placement error of support 100 is detected. In certain embodiments, support 100 such as auditory or physical gives the user support 100 correctly placed positive feedback. The positive feedback indicating that support 100 is properly placed in diagnostic equipment 300 can also indicate to the user that hinge assembly 200 has been converted into a tube retention structure, and sample tube 102 is locked in the appropriate position in sample tube holder 108.

[0136] It will be apparent that embodiments of the hinge assembly of the present disclosure can be actuated in many different ways. For example, in one non-limiting embodiment, the spacer 250 is stationary, and as the support 100 is lowered into the diagnostic device 300, the support 100 interacts with the spacer 250, thereby actuating the hinge assembly 200. In another non-limiting embodiment, the spacer 250 moves vertically from the stowed position to the engaged position to contact the hinge assembly 200, and thereby applies a force to actuate the hinge assembly 200 after the support 100 is received in the diagnostic device 300.

[0137] When the rack 100 is placed within the diagnostic device 300, the spacer 250 is shaped and dimensioned to contact the hinge plate 202 of the hinge assembly 200 to lock one or more sample tubes 102 in place. Advantageously, the sample tubes 102 are constrained in the rack 100 and in the diagnostic device 300 in a horizontal direction at least by the upper opening 144 of the sample tube holder 108, and in a vertical direction at least by the hinge assembly 200.

[0138] The support 100 can be designed so that it can be easily removed and reinserted into the diagnostic device 300. After being removed from the diagnostic device 300, the separator 250 no longer applies force on the hinge plate 202 of the hinge assembly 200. One or more sample tubes 102 can be easily removed by sliding one or more sample tubes 102 vertically upwards. A new sample tube 102 can be easily inserted by sliding one or more sample tubes 102 vertically downwards. The support 100 can be reinserted into the diagnostic device 300. The action of inserting can actuate the hinge assembly 200 to retain a new set of sample tubes 102. The actuation of one or more separators 250 can therefore be automatic, without further action by the user. The action of engaging the hinge assembly 200 with the separator 250 can be the same action as inserting the support 100 into the diagnostic device 300.

[0139] It should be understood that embodiments of hinge assemblies according to the present disclosure can be received in a receiving compartment that does not include a divider 250. In such cases, the hinge assembly 200 can be locked in place by any suitable mechanism, including but not limited to a pin, linkage, lever, wedge, cam, or sliding rod that engages the hinge assembly 200 to pivot the hinge plate 202. In some embodiments, the mechanism is also used to lock the hinge plate 202 in a tube retaining configuration. In some embodiments, the support 100 can be loaded with one or more sample tubes 102 only before being inserted into a diagnostic device 300 such as used with a receiving compartment having a divider 250. In some embodiments, the support 100 can be loaded with one or more sample tubes 102 before and after being inserted into a diagnostic device 300 such as used with a receiving compartment having other suitable mechanisms.

[0140] Fig.18 Another example of a diagnostic device 300 according to the present disclosure is shown. According to an embodiment of the present disclosure, the diagnostic device 300 includes a receiving compartment 301 configured to receive a support 100 or multiple parts of a support 100. Fig.18 In the non-limiting embodiment illustrated in , the receiving compartment 301 is shown as being surrounded by dotted lines. In this example, the receiving compartment 301 includes a first portion 303 of a reagent housing 110 configured to receive the support 100. The receiving compartment 301 can include a second portion 304 that is separate from and adjacent to the first portion 303. The second portion 304 can include an open volume, hole or compartment that is separate from and adjacent to the first portion 303. The support 100 can be designed so that it can be easily inserted and removed from the diagnostic device 300, such as the reagent housing 110 can be inserted in the first portion 303 of the receiving compartment 301, and the sample tube holder 108 can be inserted in the second portion 304 of the receiving compartment 301. As described herein, the reagent housing 110 can include one or more alignment members 130 that facilitate the positioning of the support 100 in one or more recesses 306 in the diagnostic device 300. The registration feature 130 is configured to ensure that the holder 100 can only be placed in the diagnostic device 300 in a single orientation such that the first portion 303 receives the reagent housing 110 .

[0141] The receiving compartment 301 can include a divider 250, Fig.18In the non-limiting embodiment illustrated in , the divider 250 is shown as being surrounded by a dotted line. The divider 250 is located between the first portion 303 of the receiving compartment 301 and the second portion 304 of the receiving compartment 301. The divider 250 can be stationary in the receiving compartment 301, such as an integral wall portion of the receiving compartment 301. As the support 100 is lowered into the receiving compartment 301 of the diagnostic device 300, for example, as the reagent housing 110 is inserted into the first portion 303 and as the sample tube holder 108 is inserted into the second portion 304, the support 100 interacts with the divider 250. The divider 250 actuates the hinge assembly 200 as described herein.

[0142] Example Sample Tube and Reagent Holder Loading Procedure

[0143] An example sample tube and reagent holder loading procedure according to a first embodiment of the present disclosure will now be described. In some embodiments, the rack 100 is loaded with one or more sample tubes 102 on a work surface. The rack 100 can be designed so that it can be easily loaded with one or more sample tubes 102, such as in Figures 15A-15C . The loading of the sample tube 102 can be intuitive because the sample tube 102 is vertically inserted into the vertically aligned openings 144, 156. The hinge plate 202 is pivoted away from the openings 144, 156 to allow the sample tube 102 to be loaded.

[0144] The sample tube holder 108 can include aligned upper and lower openings 144, 156 that allow for insertion of the sample tube 102. The upper and lower openings 144, 156 can be aligned along a vertical axis so that the sample tube 102 can be inserted along the vertical axis. Fig.15A The vertical axis A shown in FIG. 1 is inserted. The upper opening 144 can support and guide the sample tube 102 during further insertion. The lower opening 156 can support and guide the sample tube 102 during further insertion.

[0145] In some embodiments, the reagent housing 110 of the rack 100 is loaded with one or more reagent holders 104 prior to being inserted into the diagnostic device 300. In some embodiments, the rack 100 is loaded with one or more reagent holders 104 on a work surface.

[0146] In some embodiments, the support 100 is designed to be stable on a horizontal surface, such as a workbench surface on which one or more sample tubes 102 and one or more reagent holders 104 are loaded. The support 100 is easily tipped over during transport, and for this purpose, the support 100 has one or more feet 124 symmetrically attached to the first and second vertical members 134 of the reagent housing 110. In certain embodiments, the support has a handle 126 that is easy to lift and move, and in some embodiments, the handle 126 can be locked into a vertical position during transport. In some embodiments, the handle 126 is positioned around an axis displaced from an axis passing through the center of the support 100 when loaded. The handle 126 is designed to rest on a position flush with the upper horizontal member 132 of the reagent housing 110.

[0147] The sample tube 102 can include additional features that facilitate the sample preparation process. The sample tube 102 is designed to hold and transport samples for various purposes. In a non-limiting embodiment, the sample tube 102 is used in sample preparation in a clinical setting. The sample tube 102 can have any suitable shape and size.

[0148] Example sample tubes and sample tube holders

[0149] The features of the example sample tube and the sample tube holder used for use with the embodiments of the present disclosure will now be described.It should be understood that in the embodiments of the present disclosure, the following example sample tube is non-restrictive, and any suitable sample tube can be used.In some cases, all sample tubes 102 loaded in the sample tube holder 108 are exactly the same.In some instances, two or more sample tubes 102 loaded in the sample tube holder 108 are the same size, shape and configuration.In other cases, two or more sample tubes 102 loaded in the sample tube holder 108 are different sizes, shapes and configurations.The diameter and length of the sample tube 102 can change.For example, two or more sample tubes 102 loaded in the sample tube holder 108 can have the same diameter.As another example, two or more sample tubes 102 loaded in the sample tube holder 108 can have different diameters.The sample tube with different features received in the support 100 can interact with the sample tube holder 108 and the hinge assembly 200 in different ways. For example, one sample tube 102 may be in contact with the fourth surface 160 of the sample tube holder 108, and one sample tube 102 may not be in contact with the fourth surface 160 of the sample tube holder 108. In some embodiments, the sample tube holder 108 is designed to hold exactly the same sample tube 102. In some embodiments, the second sample tube holder 108 is designed to hold exactly the same sample tube 102 of a different size, shape, or configuration than the first sample tube holder 108. In some embodiments, the sample tube holder 108 is interchangeable with or replaceable with a second sample tube holder 108 of the rack 100. In some embodiments, the sample tube holder 108 is coupled to the rack 100, and the second sample tube holder 108 is coupled to the second rack 100.

[0150] The sample tube 102 is a self-contained container that can prevent fluid leakage and minimize cross sample contamination. The sample tube 102 can include a sealing cap that allows the sample tube 102 to be transported. The sealing cap prevents any liquid from escaping by providing a liquid-tight seal. After the sample has been added to the sample tube 102, the sealing cap can be coupled to the sample tube 102 by the manufacturer or by a care provider. In certain embodiments, the proximal end 170 of the sample tube 102 includes a threaded surface. The sealing cap can engage the threaded surface to removably couple the sealing cap to the sample tube 102. The sample tube 102 and the sealing cap can be any commercially available sample tube system.

[0151] The sample tube 102 can include a cap 172 that allows the sample tube 102 to be penetrated by a fluid transfer device (such as a pipette tip). In some embodiments, the cap 172 may not provide a liquid-tight seal. The cap 172 can be coupled to the sample tube 102 at a location where sample preparation is to be performed (e.g., at a location of the diagnostic device 300). In some embodiments, the proximal end 170 of the sample tube 102 includes a threaded surface. The cap 172 can engage the threaded surface to removably couple the cap 172 to the sample tube 102.

[0152] Cap 172 can allow access to the sample by a liquid dispenser. In certain embodiments, cap 172 includes a threaded exterior designed to engage sample tube 102. In certain embodiments, cap 172 includes a penetrable diaphragm designed to receive a pipette tip therethrough. The diaphragm can include features that substantially limit accidental fluid transfer, evaporation, or leakage. The diaphragm can include features that wipe the pipette tip during withdrawal. Cap 172 can allow access to the sample to allow one or more amplification prepared samples to be prepared from the sample. Sample tube 102 with cap 172 is configured to be used in a diagnostic device 300 that can simultaneously prepare samples in more than one sample tube 102 for sample preparation.

[0153] The sample tube 102 for use with the support 100 can be made of any suitable material, including but not limited to plastic. The sample tube 102 can be sufficiently rigid so that the sample tube 102 is not easily deformed during routine handling and transportation. The tubular portion of the sample tube 102 can be made of a single piece (such as a whole or integral structure), wherein the cap 172 is made of a separate structure. The sample tube 102 can be translucent. The sample tube 102 can be disposable, such as intended for a single use, after which the sample tube 102 is discarded.

[0154] Example Liquid Dispenser

[0155] The sample tube 102 and the reagent holder 104 are configured to receive pipetting operations performed manually by an operator and by a liquid dispenser 302 when received by the rack 100. The liquid dispenser 302 controls fluid handling operations of any fluid, including fluid samples and in particular a variety of biological samples. The liquid dispenser 302 performs various aspiration and dispensing operations on the sample tube 102 and the reagent holder 104. The liquid dispenser 302 can include a pipette tip sized to penetrate the cap 172 of the sample tube 102.

[0156] In general, non-limiting features of the liquid dispenser 302 suitable for operating the sample tube 102 and the reagent holder 104 according to the present disclosure include at least: picking up the pipette tip and returning the pipette tip after use; grabbing and discarding the pipette tip from the liquid dispenser 302 after use or when an error is encountered; and accurately moving the pipette tip from one position to another position of a given support 100. For example, the sample from the sample tube 102 can be mixed with one or more reagents, the liquid reagent can be added to the solid reagent to form a solution, and the various liquid reagents and samples can be mixed with each other during the sample preparation protocol. When received by the support 100, the liquid dispenser 302 can operate two or more sample tubes 102 individually or simultaneously. When received by the support 100, the liquid dispenser 302 can operate two or more reagent holders 104 individually or simultaneously. The liquid dispenser 302 can operate two or more channels 106 individually or simultaneously. The liquid dispenser 302 can perform certain operations in parallel. The liquid dispenser 302 can perform certain operations continuously.The liquid dispenser 302 can move in multiple degrees of freedom, such as at least three degrees of freedom.

[0157] The diagnostic device 300 can be designed to carry out automated sample preparation to a plurality of samples contained in the sample tube 102 according to the steps exemplified herein. The geometrical arrangement of the components of the diagnostic device 300 is schematic and is not intended to be limiting. Embodiments of the present disclosure can be implemented in any suitable diagnostic device in which the sample tube is received in the sample tube holder. The diagnostic device 300 can additionally include a microfluidic box 308 in the box receiving compartment 305. The microfluidic box 308 can be configured to amplify the sample and detect the presence of the amplified polynucleotides in the microfluidic box 308. The liquid dispenser 302 can be configured to obtain aliquots of a fluid containing an analyte of interest (such as, but not limited to, nucleic acids extracted from one or more samples) and direct them to other areas of the diagnostic device (such as, but not limited to, a storage area).

[0158] Diagnostic equipment 300 can include processor 310, such as microprocessor, and described processor 310 is configured to the function of the various parts of control diagnostic equipment 300, and communicates with each such parts of need control thus.Liquid distributor 302 can be controlled by processor 310.Liquid distributor 302 is configured to carry out various aspiration and distribution operations to the corresponding sample, fluid and reagent in sample tube 102 and reagent holder 104.Liquid distributor 302 can carry out this type of operation to a plurality of sample tubes 102 and reagent holder 104 simultaneously.Diagnostic equipment 300 can handle a plurality of samples in parallel, and wherein each passage 106 experiences independent or independent step or process.In addition, the order that various functions are hereinafter described is not to limit the order of processor 310 execution instructions when diagnostic equipment 300 is operating.

[0159] The diagnostic device 300 is configured to operate in conjunction with a complementary stent 100. In some embodiments, the diagnostic device 300 may be capable of receiving a plurality of stents 100. Fig.17 Two supports 100 in diagnostic equipment 300 are illustrated.Support 100 is configured to receive multiple biological samples in sample tube 102, and prepares these samples in a form suitable for post-processing (work-up) and diagnostic analysis.Support 100 is configured to receive multiple reagents in reagent holder 104, and the reagent holder 104 can be equipped with various parts, optionally including treatment tube 174, reagent tube 176 and receptacle 180 with container.Support 100 is configured so that, during sample post-processing, sample is processed in the corresponding reagent holder 104 in the corresponding channel 106 of sample tube 102.In certain embodiments, the processing relates to mixing, heating, cooling and / or magnetic separation.

[0160] The diagnostic device 300 can be self-contained and operate in conjunction with a sample tube 102 and a reagent holder 104 that are inserted into the diagnostic device 300 via a rack 100. The diagnostic device 300 can be configured for multiplexed sample analysis and / or analysis of multiple batches of samples, with a single rack 100 holding a single batch of samples. Each component of the diagnostic device 300 can therefore be present as many times as there are batches of samples, but the various components can be configured in a common housing.

[0161] A second exemplary bracket according to the present disclosure

[0162] 19-24 illustrate a stent 400 according to a second embodiment of the present disclosure. Fig.19A A front perspective view of the bracket 400 is shown. Fig.19B An exploded front perspective view of the bracket 400 is shown. Figures 20A-20B A rear perspective view of the bracket 400 is shown. Fig.21 A top view of the bracket 400 is shown. Fig. 22 A side view of the bracket 400 is shown. Fig.23 A front view of the bracket 400 is shown. Fig.24 A rear view of the support 400 is shown. The support 400 is configured to receive a plurality of sample tubes 102, and to receive a plurality of reagent holders 104. The support 400 receives these components in such a manner that the sample tubes 102 and the reagent holders 104 can be loaded individually and independently from each other. In this non-limiting embodiment, the sample tubes 102 and the reagent holders 104 are one-to-one corresponding. The support 400 is configured to receive twelve sample tubes 102 and twelve corresponding reagent holders 104. In certain embodiments, the support 400 can receive 1, 2, 4, 6, 8, 10, 12, 16, 20, 24, 36, 48, 60 or 72 sample tubes 102, and therefore the support 400 can receive 1, 2, 4, 6, 8, 10, 12, 16, 20, 24, 36, 48, 60 or 72 corresponding reagent holders 104. In certain embodiments, the support 400 can receive any suitable number of samples. In some embodiments, rack 400 can receive any suitable number of reagent holders.Rack 400 can include any of the features of rack 100 described herein.

[0163] The rack 400 can include two or more sub-components. The rack 400 includes a sample tube holder 408 configured to receive one or more sample tubes 102. See the non-limiting example of a sample tube 102. Figure 1-3 In this non-limiting example, the rack 400 also includes a reagent housing 410 configured to receive one or more reagent holders 104. See the non-limiting example of a reagent holder 104. Figure 1-3 The sample tube holder 408 and the reagent housing 410 can be coupled to form a unitary structure. The sample tube holder 408 can include any of the features of the sample tube holder 108 described herein. The reagent housing 410 can include any of the features of the reagent housing 110 described herein. The rack 400 can be divided into one or more channels 406. The channels 406 can have any of the features of the channels 106 described herein.

[0164] The reagent housing 410 comprises a horizontal member 432 and two vertical members 434 connected to the horizontal member 432. Each of the vertical members 434 comprises two feet 424, but other configurations of the vertical members 434 and feet 424 are contemplated. Any suitable arrangement of the horizontal and vertical members can be implemented. The two vertical members 434 are configured to allow the reagent housing 410 to stand or maintain stably. The reagent housing 410 can include a handle 426.

[0165] The reagent housing 410 includes a reagent housing edge 436. The reagent housing edge 436 is where the horizontal portion 436a and the vertical portion 436b of the reagent housing 410 meet. The reagent housing edge 436 can be a ninety degree edge or a right angle edge. The reagent housing edge 436 can be the edge of the horizontal member 432.

[0166] The reagent housing edge 436 is arranged so that it faces the sample tube holder 408, as described herein. The reagent housing 410 includes a first set of openings 438. The first set of openings 438 can include one or more openings. In the illustrated embodiment, the first set of openings 438 includes two openings. The first set of openings 438 is designed to couple with the sample tube holder 408 described herein.

[0167] The reagent housing 410 includes Fig.19B 4. The second set of openings 440 shown in FIG. The second set of openings 440 can include one or more openings. In the illustrated embodiment, the second set of openings 440 includes three openings. The second set of openings 440 is designed to be coupled to the hinge assembly described herein. The first set of openings 438 can be closer to the two vertical members 434. The second set of openings 440 can be disposed between the first set of openings 438.

[0168] Fig.25 and 26 A non-limiting example sample tube holder 408 is illustrated in accordance with the present disclosure. The sample tube holder 408 includes features to position and retain the sample tubes 102. The rack 400 is configured to receive a plurality of samples, each sample being received in an individual sample tube 102. Fig.25 A front perspective view of the sample tube holder 408 is shown, and Fig.26 Shown is a rear perspective view of the sample tube holder 408. The sample tube holder 408 can have any of the features of the sample tube holder 108 described herein.

[0169] The sample tube holder 408 includes a first portion 442, a second portion 446, a third portion 454, and a fourth portion 460. The first portion 442 is flat or substantially flat. The first portion 442 includes a plurality of upper openings 444. The upper openings 444 can be rounded or circular, but can have other cross-sectional shapes, depending on the characteristics of the sample tube 102.

[0170] The sample tube holder 408 includes a second portion 446. The second portion 446 is vertical or substantially vertical. The second portion 446 includes an upper post 448. The upper post 448 can extend vertically upward from the first portion 442. Each upper post 448 has an opening 450 configured to receive a fastener 412 therethrough. The second portion 446 can include one or more guide rails 452.

[0171] The sample tube holder 408 is configured to couple with the reagent housing 410 so that the two components of the rack 400 are rigidly connected. The openings 450 of the sample tube holder 408 are configured to align with the first set of openings 438 in the reagent housing 410. Any suitable fasteners 412 can extend through the openings 450 of the sample tube holder 408 and the corresponding openings in the reagent housing 410 to couple the sample tube holder 408 to the reagent housing 410. Fig.23 Fastener 412 is illustrated in FIG.

[0172] The sample tube holder 408 includes a third portion 454. In this example, the third portion 454 is oriented at a diagonal between the second portion 446 and the fourth portion 460. The third portion 454 includes a plurality of lower openings 456. The lower openings 456 can have any suitable cross-sectional shape, including but not limited to elliptical elongated openings, oval openings, and circular openings. The lower openings 456 are shaped and configured to accommodate the circumference of the sample tube 102.

[0173] The sample tube holder 408 includes a fourth portion 460. One or more portions of the fourth portion 460 can be horizontal or substantially horizontal. The fourth portion 460 can form a base of the sample tube holder 408. The fourth portion 460 can include one or more rails 464.

[0174] In a method of use according to the present disclosure, each of a plurality of sample tubes 102 is inserted into a sample tube holder 408. The distal end 166 of the sample tube 102 is first inserted into the upper opening 444 of the first portion 442. The distal end 166 of the sample tube 102 then passes vertically, parallel to the second portion 446. The distal end 166 of the sample tube 102 is next inserted into the lower opening 456 of the third portion 454. The distal end 166 of the sample tube 102 then passes vertically to rest on a surface of the fourth portion 460. When the sample tube 102 is received therein, the proximal end 170 of the sample tube 102 extends above the first portion 442 of the sample tube holder 408. The proximal end 170 of the sample tube 102 can include a cap 172. The cap 172 extends above the first portion 442. See an embodiment of a sample tube 102 including a distal end 166, a proximal end 170, and a cap 172. Figure 7 .

[0175] The upper opening 444 and the lower opening 456 can restrict movement in the horizontal direction based on the shape of the openings 444, 456 being similar to the outer surface of the sample tube 102. Advantageously, when disposed within the sample tube holder 408, embodiments of the present disclosure allow portions of the sample tube 102 to be accessible to a sample identifier, such as a barcode reader.

[0176] Example hinge assembly according to a second embodiment of the present disclosure

[0177] An exemplary hinge assembly 500 according to a second embodiment of the present disclosure will now be referred to. Figure 27-34 It should be understood that the hinge assembly of the present disclosure is not limited to the features of the example hinge assembly 500 and can take other forms, shapes and sizes consistent with the present disclosure. Fig. 27 A front perspective view of hinge assembly 500 is shown. Fig.28 A rear perspective view of hinge assembly 500 is shown. Fig.29 A front exploded view of hinge assembly 500 is shown. Figures 30A-30B A front view of hinge assembly 500 is shown. Fig.31 A top view of hinge assembly 500 is shown. Fig.32 A view of a portion of hinge assembly 500 is shown. Fig.33 A view of a portion of hinge assembly 500 is shown. Fig.34 A view of a portion of hinge assembly 500 is shown.

[0178] The hinge assembly 500 includes a tube insertion structure. In the tube insertion structure, the hinge assembly 500 can allow the sample tube 102 to move in the vertical direction in the sample tube holder 408. The sample tube 102 can be freely inserted into the sample tube holder 408 or removed from the sample tube holder 408. The sample tube 102 is moved vertically or substantially vertically to insert the sample tube 102 into the sample tube holder 408. This method can be repeated to vertically insert multiple sample tubes 102 into the sample tube holder 408. In some methods of use, the sample tube 102 is moved vertically or substantially vertically to remove the sample tube 102 from the sample tube holder 408. In some methods of use, the bracket is reversed to remove the sample tube 102 from the sample tube holder 408.

[0179] Hinge assembly 500 includes a tube retention structure. In the tube retention structure, hinge assembly 500 can prevent or limit the movement of sample tube 102 in the sample tube holder 408 along the vertical direction. In certain embodiments, hinge assembly 500 can prevent or limit sample tube 102 from experiencing vertical lifting during pipetting, thereby increasing pipetting efficiency. In some cases, there is a certain amount of vertical lifting of sample tube 102 during pipetting (e.g., during the exit of the pipette tip from sample tube 102). In these cases, the hinge assembly 500 in the tube retention structure stops sample tube 102 at a specific moment of the vertical travel of sample tube 102, especially when sample tube 102 is in physical contact with hinge assembly 500 and is physically prevented from further vertical lifting by hinge assembly 500. In these cases, hinge assembly 500 can prevent sample tube 102 from vertically lifting to the extent that pipetting will be hindered or completely stopped due to the failure of liquid dispenser. Advantageously, the sample tube 102 is vertically constrained by the hinge assembly 500 .

[0180] Hinge assembly 500 includes hinge support 502. Hinge support 502 is a horizontal sliding mounting. Hinge support 502 is an elongated member. In the illustrated embodiment, hinge support 502 is roughly rectangular in shape. Hinge support 502 includes front surface 504, rear surface 506 and side surface 508. Front surface 504 faces sample tube 102 in use. Rear surface 506 faces reagent housing 410 in use. Rear surface 506 can be flat to abut against vertical portion 436b of reagent housing 410. Rear surface 506 can be any feature that allows a firm connection between hinge support 502 and reagent housing 410 when fastened together. Hinge support 502 can be any material, including but not limited to metal and plastic.

[0181] The hinge support 502 has one or more openings 510 configured to receive the fasteners 414. The one or more openings 510 extend from the front surface 504 to the rear surface 506. Although three openings 510 are illustrated, the hinge support 502 can include any number of openings 510. Any segment of the hinge support 502 can include one or more openings 510. The openings 510 are configured to align with the second set of openings 440 in the reagent housing 410. When the fasteners 414 extend through the corresponding openings 510, 440, the hinge support 502 of the hinge assembly 500 is rigidly coupled to the reagent housing 410. The intermediate fasteners 414 can also be coupled to self-locking lugs, as described herein. Fig.23 Fastener 414 is illustrated. Fig.19B Fasteners of bracket 400 are illustrated.

[0182] The hinge support 502 includes a cutout 512. Although two cutouts 512 are illustrated, the hinge support 502 can include any number of cutouts 512. The cutout 512 includes an upper portion. The upper portion of the cutout 512 includes an upper opening 514. The upper opening 514 can be a generally rectangular opening of the cutout 512. The upper opening 514 extends from the front surface 504 to the rear surface 506. The upper opening 514 extends through the hinge support 502. In other embodiments, the upper opening 514 extends through a portion of the hinge support 502.

[0183] The cutout 512 includes a lower portion. The lower portion of the cutout 512 includes a lower opening 516. The lower opening 516 can be a generally rectangular opening. The lower opening 516 can be smaller than the upper opening 514. The lower opening 516 can be centered relative to the upper opening 514. The lower opening 516 can extend from the front surface 504 through a portion of the hinge support 502. In some embodiments, the lower opening 516 of the lower portion of the cutout 512 does not extend through the rear surface 506. In such embodiments, the lower opening 516 only extends through a portion of the hinge support 502. In other embodiments, the lower opening 516 extends through the hinge support 502.

[0184] The lower portion of the cutout 512 includes a groove 518. The groove 518 can be a generally rectangular groove. The groove 518 can be smaller than the upper opening 514. Fig. 27 506. The groove 518 can be to the right of the lower opening 516 when viewed from the front of the hinge assembly 500 shown in FIG. 50. The groove 518 is disposed between the front surface 504 and the rear surface 506. In some embodiments, the groove 518 does not extend through the front surface 504. In some embodiments, the groove 518 does not extend through the rear surface 506. The groove 518 can form a snap fit, as described in more detail below.

[0185] The hinge support 502 can include one or more holes 520. One or more holes 520 can be coaxial. Although two holes 520 are shown, the hinge support 502 can include any number of holes 520. The hole 520 extends horizontally through the hinge support 502. The hole 520 extends inward from the side surface 508 toward the center of the hinge support 502. The hole 520 ends at the upper opening 514 and then continues past the upper opening 514 toward the center of the hinge support 502. In the illustrated example, each side surface 508 of the hinge support 502 can include a hole 520. In other examples, the hinge support 502 includes a single hole 520 extending between the side surfaces 508 of the hinge support 502.

[0186] The hinge assembly 500 includes one or more hinge pins 522. One or more hinge pins 522 can be coaxial. Although two hinge pins 522 are illustrated, the hinge assembly 500 can include any number of hinge pins 522. The number of hinge pins 522 can correspond to the number of holes 520. In some embodiments, the hinge assembly 500 can include a single hole 520 and a single hinge pin 522. In some embodiments, the hinge assembly 500 can include multiple hinge pins 522 and a corresponding number of holes 520. In the illustrated embodiment, each hole 520 is configured to receive a hinge pin 522. The hinge pin 522 can be a substantially cylindrical member, and the corresponding hole 520 can be cylindrical. The hinge pin 522 can provide a pivot axis around which the components of the hinge assembly 500 can pivot. The hinge pin 522 can be disposed in the upper opening 514. The hinge pin 522 can be centered relative to the upper opening 514. Hinge pin 522 can be any material, including stainless steel.

[0187] The hinge assembly 500 includes one or more springs 524. One or more springs 524 can be coaxial. Although two springs 524 are illustrated, the hinge assembly 500 can include any number of springs 524. The number of springs 524 can correspond to the number of cutouts 512 in a one-to-one correspondence. The number of springs 524 can correspond to the number of hinge pins 522 in a one-to-one correspondence. Any suitable spring 524 can be implemented in an embodiment of the present disclosure. The spring 524 can be a compression spring. The spring 524 can be designed to apply a biasing force to restore a neutral configuration. The spring 524 can be any material, including stainless steel. The spring 524 can be a 0.218" outer diameter spring. The spring can be a 1" length spring. The hinge assembly 500 can include one or more washers 526. The washers 526 can distribute the force of the springs 524. The number of washers 526 can correspond to the number of springs 524 in a one-to-one correspondence. The hinge assembly 500 can include one or more caps 528. The caps 528 can retain the hinge pins 522 within the holes 520. The number of caps 528 can correspond to the number of hinge pins 522 in a one-to-one correspondence.

[0188] Hinge assembly 500 includes a slide lock 530. Features of a non-limiting example of slide lock 530 will now be described, but it should be understood that the present disclosure is not limited to this example and any suitable slide lock can be implemented according to the present disclosure. Slide lock 530 includes a front surface 532, a rear surface 534 and a side surface 536. The front surface 532 faces the sample tube 102 during use. The rear surface 534 faces the reagent housing 410 during use. The slide lock 530 can include a top surface 538 and a bottom surface 540. The slide lock 530 can be any material, including plastic.

[0189] The top surface 538 of the sliding lock 530 can form a ledge. The top surface 538 can form a right angle ledge. The top surface 538 can include a lip 542 extending upward. The lip 542 can form a portion of the rear surface 534. The lip 542 can be a generally rectangular block. The top surface 538 has one or more openings 544 configured to receive a fastener. The one or more openings 544 extend from the top surface 538 toward the bottom surface 540. Although two openings 544 are shown, the sliding lock 530 can include any number of openings 544.

[0190] The sliding lock 530 includes one or more holes 546. Although one hole 546 is shown, the sliding lock 530 can include any number of holes 546. The hole 546 extends horizontally through the sliding lock 530. The hole 546 extends through the side surface 536. In the illustrated example, the sliding lock 530 includes a single hole 546 extending between the side surfaces 536 of the sliding lock 530. The hole 546 can extend along the middle portion of the sliding lock 530. The axis of the hole 546 can be transverse to the axis of the opening 544. In some embodiments, the hole 546 and the opening 544 intersect. In some embodiments, the hole 546 and the opening 544 do not intersect. The hole 546 can extend along the rotation center of the sliding lock 530. The hole 546 is configured to receive the hinge pin 522 through the hole 546.

[0191] The rear surface 534 of the sliding lock 530 can form a shelf-like protrusion. The rear surface 534 can form a right-angle shelf-like protrusion. The rear surface 534 can include a notch 548 extending inwardly. The notch 548 can form a part of the rear surface 534. The notch 548 can be a substantially rectangular notch. In some embodiments, the notch 548 and the lip 542 have the same or similar thickness. The notch 548 can extend parallel to the hole 546. The notch 548 can extend along the middle part of the sliding lock 530. The notch 548 can be used as a stopper to keep the hinge assembly 500 in the tube insertion configuration, as described herein. The rear surface 534 can be shaped so as to slide linearly relative to the reagent housing 410. The rear surface 534 can be substantially flat. The rear surface 534 can slide relative to the flat surface of the reagent housing 410.

[0192] The sliding lock 530 includes a flange 550. The flange 550 can be offset inwardly from the front surface 532. The flange 550 can be offset inwardly from the rear surface 534. The flange 550 can be centered between the front surface 532 and the rear surface 534. The flange 550 can be offset inwardly from the side surfaces 536. The flange 550 can be centered between the side surfaces 536. The flange 550 can form the bottom surface 540 of the sliding lock 530. The flange 550 can be a generally rectangular block. The flange 550 can include one or more rounded edges.

[0193] The sliding lock 530 can be assembled relative to the hinge pin 522 and the hinge support 502. The hinge pin 522 can be inserted into the hole 520 of the hinge support 502 near the side surface 508. The spring 524 can be disposed in the upper opening 514. The hinge pin 522 can be inserted through the spring 524. The washer 268 can be disposed in the upper opening 514. The hinge pin 522 can be inserted through the washer 526. The sliding lock 530 can be positioned in the cutout 512. The flange 550 can be disposed in the lower portion of the cutout 512. The flange 550 can be disposed in the lower opening 516 and / or the groove 518. The hinge pin 522 can be inserted through the sliding lock 530. The hinge pin 522 can be inserted into the hinge support 502. The hinge pin 522 can be fixed with a cap 528. Another hinge pin 522 can be inserted into a hole 520 of the hinge support 502 adjacent the other side surface 508 and through a second sliding lock 530 in a similar manner.

[0194] The sliding lock 530 is capable of sliding relative to the hinge pin 522. The sliding lock 530 is capable of sliding in two directions that are generally parallel to the longitudinal axis of the hinge support 502. The upper portion of the sliding lock 530 is capable of sliding within the upper opening 514 of the cutout 512. The flange 550 is capable of sliding within the lower opening 516. The flange 550 is capable of sliding within the groove 518. The groove 518 is sized to allow the flange 550 to slide therein. During the sliding action of the sliding lock 530, the hinge support 502 is configured to remain stationary. The sliding lock 530 is configured to slide relative to the fixed hinge support 502.

[0195] The slide lock 530 can slide in two directions. In a first direction, the slide lock 530 compresses the spring 524 against the inner surface of the upper opening 514. Fig. 30A In the illustrated embodiment shown in FIG, when the slide lock 530 slides to the left, the slide lock 530 compresses the spring. In the second direction, the slide lock 530 decompresses the spring 524. The spring 524 can bias the spring lock 530 to move in the second direction. Fig. 30A In the illustrated embodiment shown in FIG. 5 , when the slide lock 530 slides to the right, the slide lock 530 decompresses the spring.

[0196] When the flange 550 is positioned in the lower opening 516, the sliding lock 530 can rotate around the hinge pin 522. The flange 550 can rotate from a position aligned with the groove 518 to a position skewed relative to the groove 518. The flange 550 can rotate through the lower opening 516. When the spring 524 is compressed, the sliding lock 530 can rotate around the hinge pin 522. When the sliding lock 530 is slid to the right, the sliding lock 530 can rotate around the hinge pin 522. After sliding the sliding lock 530 to align the flange 550 with the lower opening 516, the sliding lock 530 can rotate around the hinge pin 522. In the tube insertion configuration, the flange 550 is not positioned in the groove 518 or constrained by the groove 518. In the tube insertion configuration, flange 550 has been rotated from a position within lower opening 516 (aligned with but not disposed within groove 518 ) to a position where flange 550 passes through a plane including front surface 504 . Fig. 30B The flange 550 is illustrated rotated out of the lower opening 516 in the tube insertion configuration.

[0197] To transition to the tube retention configuration, the slide lock 530 can be rotated to align the flange 550 with the groove 518. In some embodiments, the slide lock 530 is slid into the groove 518 by the user. In some embodiments, the spring 524 can bias the flange 550 to slide into the groove 518. Fig. 30B 502 is configured to remain stationary during the sliding motion. The sliding lock 530 is configured to slide relative to the fixed hinge support 502.

[0198] Hinge assembly 500 includes hinge plate 552. As will be described in more detail below, hinge plate 552 can be rigidly coupled to two slide locks 530 so that in use, sliding and pivoting movement of slide lock 530 relative to hinge support 502 causes sliding and pivoting movement of hinge plate 552 relative to hinge support 502. Hinge plate 552 includes front surface 554, rear surface 556 and side surface 558. Rear surface 556 faces reagent housing 410 in use. Hinge plate 552 can include top surface 560 and bottom surface 562. Hinge plate 552 can be flat or substantially flat. Top surface 560 and bottom surface 562 can be parallel. Hinge plate 552 can have a constant thickness between top surface 560 and bottom surface 562.

[0199] The hinge plate 552 includes one or more openings 564 configured to receive fasteners 566 therethrough. Although four openings 564 are illustrated, the hinge plate 552 can include any number of openings 564. A segment of the hinge plate 552 can include one or more openings 564. In the illustrated embodiment, two openings 564 are disposed near the right side of the hinge plate 552, and two openings 564 are disposed near the left side of the hinge plate 552. The one or more openings 564 extend from the top surface 560 of the hinge plate 552 to the bottom surface 562.

[0200] As described herein, the top surface 538 of the sliding lock 530 has one or more openings 544 configured to receive fasteners. The top surface 538 of the sliding lock 530 forms a shelf-like protrusion that is shaped and sized to receive a portion of the hinge plate 552. The top surface 538 of the sliding lock 530 includes a lip 542 extending upward. The lip 542 is configured to be disposed near the rear surface 556 of the hinge plate 552. The remainder of the top surface 538 of the sliding lock 530 extends along the bottom surface 562 of the hinge plate 552. The lip 542 can facilitate alignment between the sliding lock 530 and the hinge plate 552. When the hinge plate 552 abuts the lip, the openings 544, 564 are aligned. One or more openings 564 of the hinge plate 552 can be aligned with one or more openings 544 of the sliding lock 530. The fastener 566 can couple the hinge plate 552 and the sliding lock 530. The hinge plate 552 and the slide lock 530 can form an integral structure. When the fastener 566 extends through the corresponding openings 544, 564, the hinge plate 552 is rigidly coupled to the slide lock 530. The fastener 566 can be a torx screw. The fastener 566 can be an 18-8 screw. The fastener 566 can be made of any material, including stainless steel. In an alternative embodiment, the hinge plate 552 and one or more slide locks 530 are manufactured as a single integral piece, such as injection molding of any suitable plastic.

[0201] The rear surface 556 of the hinge plate 552 can include a flange 568. The flange 568 can provide support for the middle portion of the hinge plate 552. The flange 568 can prevent bending of the middle portion of the hinge plate 552. The flange 568 can be a surface that receives force and is actuated to pivot the hinge plate 552, as described in more detail below.

[0202] The front surface 554 of the hinge plate 552 can include a profiled surface. The front surface 554 includes a plurality of concave surfaces. The front surface 554 includes a plurality of minor indents 570. The hinge plate 552 is configured to prevent one or more sample tubes 102 received in the sample tube holder 408 from moving in a vertical direction. One or more sample tubes 102 are restrained by corresponding minor indents 570. In this example, the hinge plate 552 is configured to retain twelve sample tubes 102 received in the sample tube holder 408 by twelve minor indents 570, respectively. See FIG. Fig.21 and Fig.41B , which is described in more detail below. Although the hinge plate 552 is illustrated with twelve smaller notches 570 , the hinge plate 552 can include any number of smaller notches 570 .

[0203] Smaller notch 570 can be rounded. Smaller notch 570 can be semicircular or hemispherical. Smaller notch 570 can be a part of a circle. Smaller notch 570 can be a part of a circle, such as 30 °, 40 °, 50 °, 60 °, 70 °, 80 °, 90 °, 100 °, 110 °, 120 °, 130 °, 140 °, 150 °, 160 °, 170 °, 180 °, 190 °, 200 °, 210 °, 220 °, 230 °, 240 °, 250 °, 260 °, 270 ° or any range of the above values. Smaller notch 570 can be tapered. Smaller notch 570 can correspond to the number of upper openings 444 in sample tube holder 408 in a one-to-one correspondence. The smaller notch 570 can include one or more rounded edges, such as rounded corners or a curve.

[0204] The front surface 554 includes a plurality of major indents 572. The hinge plate 552 is configured to allow insertion and removal of one or more sample tubes 102 through the upper and lower openings 444, 456 of the sample tube holder 408. When the sample tubes are aligned with the corresponding major indents 572, movement of the sample tubes 102 is permitted. Fig.40C , which is described in more detail below. The hinge plate 552 is configured to allow twelve sample tubes to be received through the larger notches 572 and then enter the upper and lower openings 444, 456 of the sample tube holder 408. The hinge plate 552 is also configured to release the twelve sample tubes 102 from the sample tube holder 408 when the twelve sample tubes 102 are aligned with the larger notches 572. Although the hinge plate 552 is illustrated with twelve larger notches 572, the hinge plate 552 can include any number of larger notches 572. Multiple larger notches 572 can be placed between multiple smaller notches 570. When from Fig.31 and32 When viewed from a perspective of , each of the larger notches 572 can be disposed on the right side of the corresponding smaller notch 570 .

[0205] The larger notch 572 can be rounded. The larger notch 572 can be semicircular or hemispherical. The larger notch 572 can be a part of a circle. The larger notch 572 can be a part of a circle, such as 90°, 100°, 110°, 120°, 130°, 140°, 150°, 160°, 170°, 180°, 190°, 200°, 210°, 220°, 230°, 240°, 250°, 260°, 270°, 280°, 290°, 300°, 310°, 320°, or any range of the above values. The larger notch 572 can be tapered. The larger notch 572 can correspond to the number of sample tubes 102 in the sample tube holder 408 in a one-to-one correspondence. The larger notch 572 can include one or more rounded edges, such as rounded corners or a curve.

[0206] In some embodiments, the smaller notch 570 and the larger notch 572 can have different radii of curvature. In some embodiments, the larger notch 572 can have a larger radius of curvature than the smaller notch 570. In some embodiments, the smaller notch 570 and the larger notch 572 can have the same radius of curvature. In some embodiments, the smaller notch 570 and the larger notch 572 can be concave. The hinge plate can include one or more rounded edges connecting the smaller notch 570 and the larger notch 572, such as rounded corners or bends. The smaller notch 570 and the larger notch 572 can form a repeating pattern.

[0207] The front surface 554 can include Fig.31 and 32558 when viewed from a perspective of . In the illustrated embodiment, the smaller notch 570 closest to the left side surface 558 can be a portion of a circle that is larger than the other smaller notches 570. The smaller notch 570 closest to the left side surface 558 can be a portion of a circle, such as 30°, 40°, 50°, 60°, 70°, 80°, 90°, 100°, 110°, 120°, 130°, 140°, 150°, 160°, 170°, 180°, 190°, 200°, 210°, 220°, 230°, 240°, 250°, 260°, 270°, or any range of the above values. In the illustrated embodiment, the larger notch 572 closest to the right side surface 558 can be a portion of a circle that is larger than the other larger notches 572. The larger notch 572 closest to the right side surface 558 can be a portion of a circle, such as 90°, 100°, 110°, 120°, 130°, 140°, 150°, 160°, 170°, 180°, 190°, 200°, 210°, 220°, 230°, 240°, 250°, 260°, 270°, 280°, 290°, 300°, 310°, 320°, or any range of the above values. The side surfaces 558 can be parallel. The side surfaces 558 can include one or more rounded edges. The larger notch 572 and the smaller notch 570 can be spaced inwardly from the side surfaces 558.

[0208] The smaller notch 570 of the hinge plate 552 can be shaped and sized to allow access to the contents of the sample tube 102 when the hinge plate 552 is in the tube retention configuration. In the tube retention configuration, the smaller notch 570 does not interfere with the central area or region C of the top of the sample tube 102. The smaller notch 570 can cover a portion of the cap 172, but not the central region C. Multiple smaller notches 570 can retain multiple sample tubes 102 in the sample tube holder 408 at the same time. Multiple smaller notches 570 can collectively and simultaneously retain all sample tubes 102 positioned in the sample tube holder 408. The smaller notch 570 of the hinge plate 552 may or may not contact the sample tube 102 in the sample tube holder 408. In some embodiments, the smaller notch 570 of the hinge plate 552 can be in direct physical contact with the cap or proximal end of the sample tube 102. In some embodiments, in a locked or tube retaining configuration, the smaller notch 570 can be vertically offset from the sample tube 102. In such a case, there is a vertical space between the top of the sample tube 102 (or the cap of the sample tube 102) and the bottom of the smaller notch 570 during normal operation, and the sample tube 102 contacts the smaller notch 570 (and is constrained from further vertical movement by the smaller notch 570) only when the sample tube 102 undergoes vertical movement or lift-off during a pipetting operation.

[0209] In the tube insertion configuration, the hinge plate 552 has been slid toward the left, as from Fig. 30A and 31 The hinge plate 552 has been pivoted after sliding, as shown in Fig. 30B . In the tube insertion configuration, the larger notch 572 is disposed above the cap 172 of the sample tube 102. The larger notch 572 of the hinge plate 552 allows for the insertion or removal of the sample tube 102. In the tube insertion configuration, the larger notch 572 does not hinder the vertical movement of the sample tube 102. Multiple larger notches 572 can allow the sample tube holder 408 to release multiple sample tubes 102 individually or simultaneously.

[0210] Example self-locking lugs according to a second embodiment of the present disclosure

[0211] An example self-locking lug for use in the second embodiment of the present disclosure will now be described. It should be understood that the example of the second embodiment does not require a self-locking lug. For example, the self-locking lug can be omitted from the second embodiment, or a different self-locking lug can be suitably implemented in the second embodiment. The self-locking lug allows the hinge plate to be mechanically pivoted by the upward force of the self-locking lug. Once pivoted, the hinge plate can slide from the tube insertion configuration to the tube retention configuration under the influence of the biasing force of the spring 524. The self-locking lug can be actuated by the receiving compartment of the diagnostic device 300 to ensure that the hinge assembly 500 is in the tube retention configuration during operation within the diagnostic device 300.

[0212] Fig.35 A top view of a bracket 400 having self-locking tabs 480 is shown. Fig.36 A bottom view of a bracket 400 with self-locking tabs 480 is shown. Fig.37 A side perspective view of the self-locking lug 480 is shown. Fig.38 A side view of the self-locking lug 480 is shown. Fig.39 A rear view of the self-locking lug 480 is shown. The self-locking lug 480 includes a first portion 482, a second portion 484, a third portion 486, and a fourth portion 488. The first portion 482 can be flat or substantially flat. In this non-limiting embodiment, the first portion 482 is vertical. The second portion 484 can be flat or substantially flat. The second portion 484 can be inclined downward between the first portion 482 and the third portion 486, such as in Fig.38 The second portion 484 can form an angle (α) from the vertical, as shown in Fig.38. The angle α relative to the vertical axis can be 40°, from vertical 45°, from vertical 50°, from vertical 50°, from vertical 55°, from vertical 60°, from vertical 65°, from vertical 70°, from vertical 75°, from vertical 80° (as shown), from vertical 85°, from vertical 90° (e.g., horizontal), or any range of the above values. The transition between the first portion 482 and the second portion 484 can be rounded. The transition between the second portion 484 and the third portion 486 can be rounded. The third portion 486 can be flat or substantially flat. In this non-limiting embodiment, the third portion 486 is vertical. The first portion 482 and the third portion 486 can be parallel.

[0213] The third portion 486 has an opening 490 configured to receive a fastener 414 therethrough. As described herein, the hinge support 502 has one or more openings 510 configured to receive one or more fasteners 414. The intermediate fastener 414 can also be coupled to the self-locking lug 480. Fig.23 4. The intermediate fastener 414 is shown in the figure. Any segment of the third portion 486 can include one or more openings 490. The opening 490 is configured to align with the corresponding opening 440 in the reagent housing 410 and the corresponding opening 510 in the hinge support 502. The opening 490 is configured to align with the intermediate opening 440 of the reagent housing 410 and the intermediate opening 510 in the hinge support 502. The opening 490 is shaped and sized to allow the fastener 414 to move freely in the opening 490 but still be constrained by the opening 490. As will be described in detail below, the self-locking lug 480 is configured to move vertically relative to the fastener 414. The movement of the self-locking lug 480 is constrained by various features described in detail below. One feature that constrains the movement of the self-locking lug 480 is the inner surface of the opening 490 that is in physical contact with the fastener 414 and is constrained by the fastener 414.

[0214] The fourth portion 488 can be flat or substantially flat. In this non-limiting embodiment, the fourth portion 488 is horizontal. The transition between the third portion 486 and the fourth portion 488 can be rounded. The second portion 484 can be skewed relative to the fourth portion 488.

[0215] In use, the self-locking lug 480 is disposed between the reagent housing 410 and the hinge support 502, such as in Fig.35 The third portion 486 is disposed between the reagent housing 410 and the hinge support 502. The third portion 486 extends above and below the horizontal member 432 of the reagent housing 410, as shown in FIG. Fig.35At least a portion of the first portion 482, the second portion 484, and the third portion 486 are above the horizontal member 432, as shown in Fig.35 The fourth portion 488 is below the horizontal member 432, as shown in Fig.36 . The first portion 482 and the second portion 484 extend away from the sample tube holder 408. The fourth portion 488 extends away from the sample tube holder 408. Back to reference Fig.33 , the hinge support 502 can include a cutout 578 configured to retain the self-locking lug 480. The cutout 578 can have similar lateral dimensions as the self-locking lug 480. The cutout 578 can allow vertical movement of the self-locking lug 480 relative to the hinge support 502.

[0216] As mentioned above Fig.23 and 27 As described, the hinge support 502 has one or more openings 510 configured to receive the fasteners 414. The one or more openings 510 extend from the front surface 504 to the rear surface 506. The openings 510 are configured to align with the second set of openings 440 in the reagent housing 410. When the fasteners 414 extend through the corresponding openings 510, 440, 490, the self-locking lugs 480 can slide relative to the reagent housing 410. The openings 490 are sized to be larger than the corresponding fasteners 414. The openings 490 allow the self-locking lugs 480 to slide relative to the reagent housing 410 in the vertical direction. The openings 490 are oval in the vertical direction. The openings 490 can be any shape that allows vertical movement when the self-locking lugs 480 are coupled to the reagent housing 410.

[0217] The self-locking lug 480 can translate vertically in an upward direction until the fourth portion 488 physically contacts and is restrained by the horizontal member 432 of the reagent housing 410. The self-locking lug 480 can translate vertically in a downward direction until the first portion 482 physically contacts and is restrained by the horizontal member 432 of the reagent housing 410. The self-locking lug 480 can translate vertically until the fastener 414 abuts the upper or lower wall of the opening 510 when the self-locking lug 480 slides.

[0218] Example method of operating the hinge assembly of the second embodiment of the present disclosure

[0219] Figures 40A-40D Views illustrating the operation of the hinge assembly 500 according to the second embodiment. Fig.40A is a side view of a sample tube 102 being inserted into a sample tube holder 408. Fig.40B is a side view of the sample tube 102 fully inserted into the sample tube holder 408. Fig.40Cis a top view of the sample tube 102 and the sample tube holder 408 when the sample tube 102 is fully inserted. Fig.40D yes Fig.40B Details of Fig.40D .exist Figures 40A-40D In a first configuration, illustrated in FIG. 5 and referred to herein as the "tube insertion configuration," the hinge assembly 500 is configured to allow a sample tube 102 to be inserted into the sample tube holder 408. The following description describes the hinge assembly 500 in the tube insertion configuration.

[0220] In the tube insertion configuration, the slide lock 530 is slid to the left, as in Fig. 30A The slide lock 530 compresses the spring 524, as shown in Fig. 30B The flange 550 is on the left side of the groove 518, as shown in Fig. 30B 518. The groove 518 does not retain the flange 550. The flange 550 is positioned within the lower opening 516. The flange 550 is rotated out of the lower opening 516. The flange 550 passes through the lower opening 516 in the front surface of the hinge assembly 500. A portion of the flange 550 abuts the wall 576 of the lower opening 516. The flange 550 is tilted outward from the lower opening 516. The flange 550 is rotated forward, as shown in FIG. Fig. 30B . The flange 550 is rotated away from the lower opening 516. In some cases, the flange 550 can be positioned at an angle γ relative to the vertical axis. The angle γ can be an acute angle. The angle γ can be from 2° vertical, from 4° vertical, from 6° vertical, from 8° vertical, from 10° vertical, from 12° vertical, from 14° vertical, from 16° vertical, from 18° vertical, from 20° vertical, from 22° vertical, from 24° vertical, from 26° vertical, from 28° vertical, from 30° vertical, from 32° vertical, from 34° vertical, from 36° vertical, from 38° vertical, from 40° vertical, or any range of the above values. Although in Fig.40B and 40D The view shown in FIG. 5 appears to indicate that flange 550 is in physical contact with sample tube 102, but this is Fig.40D . In some embodiments, the flange 550 of the slide lock 530 does not extend past the cap of the sample tube 102 in the side view. In some embodiments, the slide lock 530 is positioned so that during the range of motion, the slide lock 530 never contacts the sample tube 102 (e.g., is spaced apart between the sample tubes 102).

[0221] In the tube insertion configuration, the hinge plate 552 is tilted relative to the first portion 442 of the sample tube holder 408. The hinge plate 552 is tilted away from the sample tube 102. For example, the hinge plate 552 is rotated clockwise along its longitudinal axis, such as from Fig.40B. The hinge plate 552 is tilted upward. A plurality of larger notches 572 are aligned with the upper opening 444 of the sample tube holder 408. The hinge plate 552 is rotated toward the reagent housing 410. The hinge plate 552 is rotated to provide a gap for the sample tube 102 to be inserted through the plurality of larger notches 572 and inserted into the upper opening 444 of the sample tube holder 408. In the tube insertion configuration, the hinge plate 552 is positioned to provide a gap for the sample tube 102 to be vertically inserted into the sample tube holder 408. The tube insertion configuration also allows tube removal. The hinge plate 552 is rotated to provide a gap for the sample tube 102 to be removed through the plurality of larger notches 572 and removed from the upper opening 444 of the sample tube holder 408. The hinge plate 552 is positioned to provide a gap for the sample tube 102 to be vertically removed from the sample tube holder 408.

[0222] In the tube insertion configuration, the notch 548 of the slide lock 530 can act as a stop to hold or retain the hinge plate 552 in the tube insertion configuration until actuated to change from the tube insertion configuration. When held in the tube insertion configuration, the hinge plate 552 can be spring loaded to actuate and move to the tube retention configuration. The notch 548 can abut the reagent housing edge 436, as in Fig.40B . The notch 548 can provide an additional contact point between the hinge assembly 500 and the reagent housing 410. The notch 548 can provide stability for the slide lock 530. The notch 548 can prevent further rotation of the slide lock 530. When the notch 548 abuts the reagent housing edge 436, further rotational movement in one direction can be prevented. The notch 548 can prevent the slide lock 530 from rotating clockwise about the hinge pin 522. The notch 548 can allow the slide lock 530 to rotate counterclockwise (from the hinge pin 522) about the hinge pin 522. Fig.40B 4 (view) until the notch abuts the housing edge 436. The notch 548 of the slide lock 530 can abut the reagent housing edge 436 along the surface of the notch 548. The contact between the notch 548 of the slide lock 530 and the reagent housing 410 can limit further rotation of the hinge plate 552 in the clockwise direction, such as from Fig.40A and 40B Observed from the perspective of.

[0223] The hinge pin 522 provides an axis of rotation for the rotational movement of the hinge plate 552 and the slide lock 530. In the tube insertion configuration, all other translations and rotations can be prevented or limited. In the tube insertion configuration, the hinge plate 552 and the slide lock 530 can have one degree of freedom of rotational movement. For example, as in Fig.40A As shown in FIG. 5 , the hinge plate 552 and the slide lock 530 of the hinge assembly 200 can rotate in a counterclockwise direction. Once rotated in a counterclockwise direction, the slide lock 530 can have an additional degree of freedom such as allowing sliding.

[0224] Fig.40C A top view of the hinge plate 552 in the tube insertion configuration is shown. Fig.40A and 40B , the hinge plate 552 is rotated toward the reagent housing 410 (away from the first portion 442 of the sample tube holder 408). The larger notch 572 of the hinge plate 552 provides clearance for inserting the sample tube 102 into the sample tube holder 408 along the vertical axis of the upper opening 444 in the first portion 442. Fig.40C , the larger notch 572 is laterally offset from the upper opening 444 of the sample tube holder 408 when viewed from the top of the hinge assembly 500. The distance of the lateral offset d can be a distance that allows a user to insert a sample tube 102 into the sample tube holder 408 without interference from or contact with the hinge plate 552.

[0225] The operation of the hinge assembly 500 according to the present disclosure includes moving the hinge plate 552 from Figures 40A-40D The tube insertion structure shown in the figure moves to Fig.41A and 41B A second configuration is illustrated in and referred to herein as a “tube retaining configuration.” Fig.41A is a side view of the hinge assembly 500 after actuation. Fig.41B 5 is a top view of a sample tube 102 and a sample tube holder 408 after the hinge assembly 500 has been actuated and has moved from a tube insertion configuration to a tube retention configuration. As described herein, the hinge plate 552 is coupled to two sliding locks 530 via fasteners 566. The hinge plate 552 and one or more sliding locks 530 rotate around the hinge pin 522 as a unitary structure. The hinge plate 552 and one or more sliding locks 530 slide around the hinge pin 522 as a unitary structure. The hinge plate 552 and one or more sliding locks 530 are capable of rotating and sliding relative to the stationary hinge support 502. The following description describes the hinge assembly 500 in a tube retention configuration.

[0226] In this non-limiting embodiment, in the tube retaining configuration, the hinge plate 552 is positioned in a horizontal or substantially horizontal orientation, such as in Fig.41A As shown in the figure. Fig.40A516. The hinge plate 552 and the slide lock 530 can be rotated counterclockwise from a perspective. The hinge plate 552 and the slide lock 530 can be rotated from a skewed orientation to a generally horizontal orientation. As the hinge plate 552 and the slide lock 530 rotate, the flange 550 rotates from the wall 576 adjacent to the lower opening 516 to a position where the flange 550 is completely within the lower opening 516 between the front surface 504 and the rear surface 506 of the hinge support 502. As the hinge plate 552 and the slide lock 530 rotate, the flange 550 rotates inward toward the rear surface 506. The hinge plate 552 and the slide lock 530 can be rotated until the flange 550 is vertical or generally vertical. The hinge plate 552 and the slide lock 530 can be rotated until the flange 550 is adjacent to the rear portion of the lower opening 516. The hinge plate 552 and the slide lock 530 can be rotated until the flange 550 is aligned with the groove 518. In these example embodiments, contact between the flange 550 and the rear portion of the lower opening 516 limits further counterclockwise rotation of the hinge plate 552 and the sliding lock 530 .

[0227] In this non-limiting embodiment, the hinge plate 552 and the sliding lock 530 slide along the hinge pin 522. The spring 524 applies a force to the sliding lock 530, and thus to the hinge plate 552 coupled to the sliding lock 530. The spring 524 biases the sliding lock 530 in a first direction. The spring 524 biases the sliding lock 530 to move toward the groove 518 in the lower opening 516 in the first direction, see Fig. 30A . The flange 550 slides within the groove 518 under the influence of the spring 524. When the flange 550 slides within the groove 518, the hinge plate 552 is positioned in a horizontal or substantially horizontal orientation. The hinge plate 552 and the sliding lock 530 are able to slide until the sliding lock 530 contacts the side surface of the upper opening 514. In these example embodiments, the contact between the sliding lock 530 and the upper opening 514 limits further sliding of the sliding lock 530 relative to the hinge support 502, and thereby limits further sliding of the hinge plate 552 (coupled to the sliding lock 530) relative to the hinge support 502. After this pivoting and sliding movement of the sliding lock 530 and the hinge plate 552 is completed, the hinge plate 552 is in a tube retaining configuration, such as in Fig. 30A As shown in .

[0228] When in the tube retaining configuration, the hinge plate 552 retains the sample tube 102 in the sample tube holder 408. Fig.40BIn contrast, the hinge plates 552 are pivoted and slid. The smaller notches 570 of the plurality of hinge plates 552 are vertically aligned with the sample tube 102. In some embodiments, the smaller notches 570 of the plurality of hinge plates 552 are placed on the sample tube 102 or the cap 172 of the sample tube 102. In some embodiments, the hinge plates 552 are vertically offset from the top surface of the cap 172 of the sample tube 102. In some embodiments, the hinge plates 552 have a small vertical distance between the hinge plates 552 and the top surface of the cap 172 of the sample tube 102.

[0229] The smaller notch 570 of the hinge plate 552 can be a concave surface or cutout that allows access to the contents of the sample tube 102. In the tube retention configuration, the smaller notch 570 allows access to the central area or region C of the top of the sample tube 102. In some embodiments, the central area or region C of the top of the sample tube 102 can include a foil covering. In some embodiments, the central area or region C of the top of the sample tube 102 can include a film configured to be pierced. In the tube retention configuration, the smaller notch 570 can be shaped like an outer edge or edge of the cap 172. The smaller notch 570 can retain multiple sample tubes 102 in the sample tube holder 408 at the same time. If the sample tube experiences lift-off during a pipetting operation, the smaller notch 570 can apply a vertical retention force collectively and simultaneously.

[0230] In embodiments of the present disclosure, an actuation force pivots the hinge plate 552 to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. In some embodiments, the same actuation force pivots the hinge plate 552 to move the hinge assembly 500 from the tube retention configuration. In other embodiments, a second, different actuation force can move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration.

[0231] In some embodiments, the user applies an actuation force to a portion of the hinge assembly 500 to pivot the hinge plate 552 relative to the hinge support 502 to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. In some embodiments, after the user pivots the hinge plate 552, the actuation force of the spring 524 causes the hinge plate 552 to move in a translation direction (right / left, such as from Figures 30A-30B In some embodiments, the user applies an actuation force to a portion of the hinge assembly 500 to move the hinge assembly 500 from the tube retention configuration to the tube insertion configuration. In some embodiments, the user applies a force that rotates the hinge plate 552. In some embodiments, the user applies a force that translates the hinge plate 552. In some embodiments, the user can apply a downward force on the hinge plate 552 to rotate the hinge plate 552. In some embodiments, after the user applies a pivoting force on the hinge plate 552, the actuation force of the spring 524 translates the hinge plate 552 in a translation direction.

[0232] exist Fig.41A In another example illustrated in FIG. 4 and described in detail below, a structural feature of the receiving compartment in which the bracket 400 is received applies an actuation force to the self-locking lug 480 to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. The structural feature is capable of maintaining the actuation force, thereby locking the hinge plate 552 in the tube retention configuration.

[0233] It should be understood that any portion of the self-locking lug 480 can be contacted by the actuation force to pivot the hinge assembly 500. For example, the first portion 482, the second portion 484, the third portion 486, or the fourth portion 488 can be pushed upward to move the self-locking lug 480 into engagement with the hinge plate 552. The fourth portion 488 can be flat or substantially flat. In this non-limiting embodiment, the fourth portion 488 is horizontal. In some embodiments, the fourth portion 488 of the self-locking lug 480 is contacted by the actuation force to pivot the hinge assembly 500. The self-locking lug 480 can be actuated to change the position of the hinge assembly 500. As shown in FIG. Fig.40A and 41A The hinge plate 552 is actuated to rotate the hinge plate 552 in a counterclockwise direction from the perspective of . The second portion 484 of the hinge plate 552 can be a lever arm that allows the hinge plate 552 to rotate.

[0234] Embodiments of the hinge assembly 500 can be actuated by the divider 250 in the receiving bay of the receiving bracket 400 to move from a tube insertion configuration to a tube retention configuration. Fig.18 . The divider 250 can include a surface, wall, shelf-like protrusion, enclosure, or any other suitable structure that is shaped, sized, and positioned in the receiving compartment to actuate the self-locking lug 480 when the bracket 400 is inserted into the receiving compartment. In the illustrated example, the divider 250 can include one or more shelf-like protrusions or step surfaces. For example, in a non-limiting example, the divider 250 can include one or more pins, rods, or bars that are positioned in the receiving compartment to interact with the hinge assembly 500 when the bracket 400 is inserted into the receiving compartment. The divider 250 is shaped and sized to separate the receiving compartment into two areas, a first area and a second area. When the bracket 400 is received in the receiving compartment, the reagent housing 410 is positioned in the first area, and the sample tube holder 408 is positioned in the second area. When the bracket 400 is located in the receiving compartment, the divider 250 is located between the reagent housing 410 and the sample tube holder 408.

[0235] When the bracket 400 is inserted into the receiving compartment, the divider 250 acts on the self-locking lug 480. The divider 250 contacts the fourth portion 488 of the self-locking lug 480. The divider 250 applies an upward force on the fourth portion 488 of the self-locking lug 480. The interaction of the divider 250 with the fourth portion 488 thus causes the self-locking lug 480 to slide upward. The self-locking lug 480 contacts the flange 568 of the hinge plate 552. See Fig.35 and 40C The rear surface 556 of the hinge plate 552 can include a flange 568. The flange 568 can extend away from the profiled front surface 554. In use, when the bracket 400 is assembled, the flange 568 extends over the self-locking lugs 480.

[0236] The flange 568 can be actuated to pivot the hinge plate 552. The self-locking lug 480 is pushed upward by the divider 250. The self-locking lug 480 slides relative to the fastener 414 inserted into the opening 490 of the self-locking lug 480. The second surface 484 of the self-locking lug 480 is skewed. Fig.38 As the self-locking lug 480 moves upward, the upward edge of the second surface 484 contacts the flange 568. The upward edge of the second surface 484 is located near the interface between the second surface 484 and the first surface 482. The upward edge of the second surface 484 is spaced apart from the third surface 486. The upward edge of the second surface 484 is toward the rear edge of the flange 568 to provide greater leverage to pivot the hinge plate 552.

[0237] Come back for reference Fig.18, the diagnostic device 300 includes a receiving compartment 301 configured to receive a rack 400 or multiple parts of a rack 400. In this example, the receiving compartment 301 includes a first portion 303303 configured to receive a reagent housing 410 of the rack 400. The receiving compartment 301 can include a second portion 304 that is separate from and adjacent to the first portion 303. The second portion 304 can include an open volume, hole or compartment that is separate from and adjacent to the first portion 303. The rack 400 can be designed so that it can be easily inserted and removed from the diagnostic device 300, for example, the reagent housing 410 can be inserted in the first portion 303 of the receiving compartment 301, and the sample tube holder 408 can be inserted in the second portion 304 of the receiving compartment 301. The receiving compartment 301 can include a partition 250. The partition 250 is located between the first portion 303 of the receiving compartment 301 and the second portion 304 of the receiving compartment 301. The divider 250 can be stationary in the receiving bay 301, such as an integral wall portion of the receiving bay 301. When the bracket 400 is lowered into the receiving bay 301 of the diagnostic device 300, the bracket 400 interacts with the divider 250. The divider 250 actuates the self-locking lug 480 by providing a stop that pushes the self-locking lug 480 upward. This upward movement of the self-locking lug 480 acts on the hinge plate 552 of the hinge assembly 500.

[0238] The self-locking lugs 480 cause the hinge plate 552 to rotate about the hinge support 502. After the rotation, the spring 524 causes the hinge plate 552 to slide and cause the hinge assembly 500 to transition from the tube insertion configuration to the tube retention configuration. In some embodiments, the self-locking lugs 480 cause the hinge plate 552 to pivot to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. In some embodiments, before insertion into the receiving compartment, the user pivots the hinge plate 552 to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. In some embodiments, the self-locking lugs 480 or the user provide the pivoting motion to facilitate the transition of the hinge assembly 500 from the tube insertion configuration to the tube retention configuration.

[0239] In some embodiments, the spring 524 slides the hinge plate 552 to facilitate the transition of the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. The spring 524 can provide a biasing force to slide the hinge plate 552. In some embodiments, the spring 524 slides the hinge plate 552 after the self-locking lug 480 or the user provides a pivoting motion. In some embodiments, the user pivots and slides the hinge plate 552 to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration.

[0240] When the bracket 400 is in the receiving compartment, the self-locking lugs 480 are pushed upward by the divider 250. When the bracket 400 is received in the receiving compartment, the self-locking lugs 480 limit or prevent the hinge plate 552 from pivoting into the tube insertion configuration. The self-locking lugs 480 ensure that the hinge assembly 500 is in the tube retention configuration when in the receiving compartment. The self-locking lugs 480 ensure that the hinge plate 552 is always horizontal or approximately horizontal when placed in the diagnostic device 300. Fig.17 An embodiment of a diagnostic device 300 is illustrated. The diagnostic device 300 can be configured to actuate the self-locking lugs 480 as described herein. In the tube retaining configuration, the hinge plate 552 can limit vertical movement of the sample tube 102 if the sample tube 102 is lifted during a pipetting operation.

[0241] The user can remove the bracket 400 from the receiving compartment of the diagnostic device 300. Once the bracket 400 is removed from the receiving compartment, the self-locking lug 480 no longer interacts with the partition 250 and can slide downward under the influence of gravity. The second portion 484 of the self-locking lug 480 can move downward away from the flange 568. When the bracket 400 is removed from the receiving compartment, the user can move the hinge assembly 500 from the tube retention structure to the tube insertion structure. In some embodiments, the user slides the hinge plate 552 and then pivots to move the hinge assembly 500 from the tube retention structure to the tube insertion structure. The force of the spring 524 is overcome to move the hinge assembly 500 from the tube retention structure to the tube insertion structure. In some embodiments, the user provides both the sliding and pivoting actuation forces that change from the tube retention structure to the tube insertion structure. The user can apply a sliding force, then an upward force or a clockwise force on the hinge plate 552 to move the hinge assembly 500 from the tube retention structure to the tube insertion structure. The side surface 558 of the hinge plate 552 near the right side can be easier to clamp because it is laterally offset from the nearest sample tube 102 in the tube retaining configuration. It should be understood that any portion of the hinge plate 552 or any portion of the sliding lock 530 can be contacted by the actuation force to slide or pivot the hinge assembly 500. The hinge plate 552 can be clamped near the side surface 558.

[0242] After the sample tube 102 is loaded, when the rack 400 is removed from the receiving compartment, the user is able to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration. In some embodiments, the user pivots and then slides the hinge plate 552 to move the hinge assembly 500 from the tube retention configuration to the tube insertion configuration. In some embodiments, the user pivots the hinge plate 552 and the spring 524 slides the hinge plate 552 to move the hinge assembly 500 from the tube retention configuration to the tube insertion configuration. In some embodiments, the user only provides the pivoting actuation force to transition from the tube insertion configuration to the tube retention configuration. For example, the user is able to apply a downward force or a counterclockwise force on the hinge plate 552 to cause the hinge assembly 500 to transition. Alternatively or additionally, the self-locking lug 480 is able to move the hinge assembly 500 from the tube insertion configuration to the tube retention configuration.

[0243] Hinge assembly 500 has advantages over other alternative systems designed to retain sample tube 102 in sample tube holder during pipetting operation. Advantageously, embodiments of the present disclosure reliably constrain sample tube 102 in sample tube holder and can reduce the number of tubes that undergo substantial vertical lifting. Embodiments of support 400 including hinge assembly 500 according to the present disclosure reliably retain sample tube 102 during instrument workflow. Upper opening 444 of sample tube holder 408 can prevent sample tube 102 from moving in horizontal direction. In some cases, hinge assembly 500 (specifically hinge plate 552) can prevent sample tube 102 from moving substantially in vertical direction. Sample tube can have slight vertical movement due to gap between cap 172 and hinge plate 552.

[0244] The divider 250 can lock the hinge plate 552 in the tube retention configuration, thereby locking the sample tube 102 in place and limiting vertical lifting of the sample tube 102. The divider 250 and the self-locking lug 480 interact to apply a pivoting force to the hinge plate 552. The pivoting force of the self-locking lug 480 combined with the biasing force of the spring 524 can transform the hinge plate 552 from the tube insertion configuration to the tube retention configuration. In some embodiments, the insertion of the bracket 400 into the receiving compartment ensures that the self-locking lug 480 is actuated. Advantageously, the shape, size and position of the hinge assembly 500 can be adapted to adjust the maximum retention force required to retain the sample tube 102.

[0245] Advantageously, embodiments of the present disclosure are able to reliably and consistently retain sample tubes 102 in one motion. When the partition 250 contacts the self-locking lug 480, the hinge plate 552 of the hinge assembly 500 is pivoted by the actuation force of the partition 250. In some embodiments, the descent of the support 400 relative to the partition 250 is a motion that causes the self-locking lug 480 to slide and thus pivot the hinge plate 552. The partition 250 applies an upward force on the self-locking lug 480, thereby pivoting the hinge plate 552. Once pivoted, the biasing force of the spring 524 causes the hinge plate 552 to slide. After the hinge plate 552 slides, the smaller notch 572 is placed above each sample tube 102 instead of the larger notch 570.

[0246] In the tube retaining configuration, the smaller notch 572 of the hinge plate 552 is able to contact or be spaced a small vertical distance from multiple sample tubes 102. The hinge plate 552 is able to restrain all of the sample tubes 102 in the sample tube holder 408 at the same time. The overall structure of the hinge plate 552 allows a consistent force ratio to be applied to each sample tube 102. In addition, the pivoting movement of the hinge plate 552 is repeatable, so that the same actuation force causes the same pivoting movement of the hinge plate 552. Advantageously, the hinge plate 552 is easily moved between configurations to easily load and unload batches of sample tubes during a series of diagnostic tests performed continuously using the same rack, thereby minimizing user errors and the time to load and unload racks, and increasing pipetting efficiency.

[0247] In some embodiments, in the tube retention configuration, the hinge assembly 500 allows a certain vertical movement of the sample tube 102 within the sample tube holder 408. In some embodiments, one or more sample tubes can be lifted vertically a small distance during a pipetting operation, but the presence of the hinge plate 552 prevents the sample tube from moving vertically to the extent that affects the performance of the pipetting system. In some embodiments, the hinge assembly 500 contacts the sample tube 102, such as contacting the cap 172 of the sample tube 102 with the smaller notch 572. In some embodiments, the hinge assembly 500 is placed above the cap 172 of the sample tube 102 using only the smaller notch 572. In some embodiments, in the tube retention configuration, the hinge assembly 500 prevents substantially all vertical movement of the sample tube 102 within the sample tube holder 408. In some embodiments, the hinge plate 552 is vertically positioned above a portion of the sample tube 102. In some embodiments, the hinge assembly 500 (specifically the smaller notch 572) covers a portion of the circumference of the cap 172 of the sample tube 102 without obstructing the region C of the cap 172 from the pipette tip. Embodiments of the hinge assembly according to the present disclosure include additional advantages. The hinge assembly 500 can be used with any design of the sample tube 102. The hinge assembly 500 can also be used with any design of the cap 172.

[0248] Advantageously, in the tube retaining configuration, the hinge plate 552 can prevent or limit vertical movement of the sample tube 102 within the sample tube holder 408. The hinge plate 552 can prevent or limit vertical lift-off of the sample tube 102 when the contents of the sample tube 102 are accessed by the liquid dispenser. The hinge plate 552 can prevent or limit vertical movement of the sample tube 102 within the sample tube holder 408 during fluid handling operations. The hinge plate 552 can prevent or limit vertical lifting of the sample tube 102 by the pipette tip. Pipetting operations can be performed within the diagnostic device 300. When within the diagnostic device 300, the hinge assembly 500 is in the tube retaining configuration.

[0249] Hinge assembly 500 can include several advantages. Hinge assembly 500 can be easy and intuitive to use. Hinge assembly 500 can be automatically actuated, such as by a simple action of inserting support 400 into diagnostic device 300, as described herein. Using hinge assembly 500 can be a simple self-learning process. Hinge support 502 can span reagent housing 410 or a portion thereof along the front surface of reagent housing 410. Hinge assembly can be easily actuated by a user or in some embodiments by self-locking lug 480.

[0250] The hinge assembly 500 can lock the sample tube 102 in place when the rack 400 is placed in the diagnostic device 300. The self-locking lugs 480 are locking structures that lock the hinge plate 552 in a position to retain the sample tube 102 in the rack 400. The hinge assembly 500 can facilitate complete retention of the sample tube 102 within the sample tube holder 408. The hinge assembly 500 can act as a cover over each sample tube 102. In the illustrated embodiment, all sample tubes 102 within the sample tube holder 408 are constrained under one physical part (the hinge plate 552).

[0251] Advantageously, embodiments of the present disclosure reliably and consistently unlock sample tubes in one motion. The hinge plate 552 of the hinge assembly 500 can be held in position by the divider 250 of the receiving compartment of the receiving rack 400. The rack 400 can be lifted relative to the divider 250 to relieve the force of the divider 250. The self-locking lug 480 can slide vertically away from the hinge plate 552. The user can apply a pivoting or downward force to the hinge plate 552. After pivoting the hinge plate 552, the hinge plate 552 can slide relative to the hinge support 502. The hinge assembly 500 can be automatically pivoted and slid by the user to the tube insertion configuration before the rack 400 is lowered into the diagnostic device 300 or after the rack 400 is removed from the diagnostic device 300.

[0252] In the tube insertion configuration, the hinge plate 552 is pivoted upward from the sample tube holder 408. The larger notch 570 is placed above the upper opening 444 of the sample tube holder 408. The sample tube 102 can be easily inserted without interfering with the hinge plate 552. The first sample tube 102 can be easily unloaded from the sample tube holder 408, and the next second sample tube 102 can be loaded. The hinge assembly 500 does not interfere with the loading and unloading operations of the tube. Advantageously, the hinge assembly 500 allows the user to easily load and unload the sample tube 102 into the rack 400.

[0253] Hinge assembly 500 can be backward compatible, so that the support without hinge assembly 500 can be advantageously improved to include hinge assembly.In certain embodiments, the sample tube holder of the support to be improved can be removed from the reagent housing.As described herein, the sample tube holder is configured to be coupled and separated with the reagent housing via one or more fasteners 412.The opening of the sample tube holder is configured to align with the first group of openings in the reagent housing.Any suitable fastener 412 can extend through the sample tube holder and the reagent housing to couple the sample tube holder to the reagent housing.One or more fasteners 412 can also separate the sample tube holder from the reagent housing.Any previous hinge assembly or retaining member can be removed from the support to be improved.In certain embodiments, the sample tube holder remains in position during improvement.In certain embodiments, the sample tube holder does not have to be separated from the reagent housing during the process of adding the hinge assembly to the support.

[0254] The hinge support 502 has one or more openings 510 configured to receive the fasteners 414. The one or more openings 510 extend from the front surface 504 to the rear surface 506. Although three openings 510 are illustrated, the hinge support 502 can include any number of openings 510. The openings 510 are configured to align with a second set of openings in the reagent housing to be improved. When the fasteners 414 extend through the corresponding openings, the hinge support 502 of the hinge assembly 500 is rigidly coupled to the reagent housing to be improved.

[0255] The intermediate fastener 414 can also be coupled to the self-locking lug 480. When the fastener 414 extends through the corresponding opening in the hinge assembly 500, the self-locking lug 480 and the reagent housing to be improved, the self-locking lug 480 can slide relative to the reagent housing. The opening 490 is sized to be larger than the corresponding fastener 414. The opening 490 allows the self-locking lug 480 to slide relative to the reagent housing in the vertical direction. The opening 490 can be any shape that allows vertical movement when the self-locking lug 480 is coupled to the reagent housing. In the embodiment where the sample tube holder is removed to install the hinge assembly 500, the sample tube holder can be coupled to the reagent housing. In certain embodiments, the hinge assembly 500 is installed without separating the sample tube holder from the reagent housing. In certain embodiments, a supply kit is used to improve the support. The kit can include the hinge assembly 500. The kit can include the self-locking lug 480. The kit can include the fastener 412 that couples the sample tube holder to the reagent housing. The kit can include a fastener 414 that couples the hinge assembly to the reagent housing. The kit can include a tool, such as a tool for rotating the fastener. In some embodiments, the kit can include a 1 / 16th hex screwdriver. In some embodiments, the kit can include a 1 / 16th hex l-key wrench. In some embodiments, the kit can include a thread locking adhesive. In some embodiments, the kit can include 242. The kit can include any additional components described herein.

[0256] Hinge assembly 500 can be directly included in support 400 such as by fixing fasteners 412, 414, as described herein. Hinge assembly 500 and self-locking lugs 480 can be easily installed between sample tube holder 408 and reagent housing 410. Hinge assembly 500 can be easily introduced into the manufacturing supply chain, or can be installed by the user at the moment of use of support. Sample tube holder 408, hinge assembly 500, self-locking lugs 480 and reagent housing 410 can form an overall structure. The overall structure can be inserted into the receiving cabin in a fluid movement, and removed from the receiving cabin in a fluid movement.

[0257] The bracket 400 can be designed so that it can be easily inserted and removed from the Fig.17 and 184 and 5. The diagnostic device 300 shown in the figure is removed. The reagent housing 410 can include one or more alignment members 430 that facilitate the positioning of the support 400. The alignment member 430 can ensure that the support 400 is inserted along the appropriate orientation actuated by the separator 250. It is hoped that the support 400 is correctly positioned in the diagnostic device 300 when the movement is restricted thereafter so that the movement of the liquid dispenser will not be impaired during the liquid handling operation. In some embodiments, the support 400 or the diagnostic device 300 can include a sensor configured to indicate the appropriate placement of the support 400 in the diagnostic device 300.

[0258] It will be apparent that embodiments of the hinge assembly of the present disclosure can be actuated in many different ways. For example, in one non-limiting embodiment, the partition 250 is stationary, and as the rack 400 is lowered into the diagnostic device 300, the rack 400 interacts with the partition 250, thereby actuating the hinge assembly 500. When the rack 400 is placed in the diagnostic device 300, the partition 250 is shaped and sized to contact the self-locking lug 480. Advantageously, the sample tube 102 is constrained in the rack 400 in the horizontal direction at least by the upper opening 444 of the sample tube holder 408, and in the vertical direction at least by the hinge assembly 500.

[0259] The support 400 can be designed so that it can be easily removed and reinserted into the diagnostic device 300. After being removed from the diagnostic device 300, the separator 250 no longer applies force on the self-locking lug 480. The hinge plate 552 can remain in the tube retention structure until actuated by the user. The user can pivot and / or slide the hinge plate 552 so that one or more sample tubes 102 can be easily removed by sliding one or more sample tubes 102 vertically upwards. A new sample tube 102 can be easily inserted by sliding one or more sample tubes 102 vertically downwards. The support 400 can be reinserted into the diagnostic device 300. The action of inserting can actuate the hinge assembly 500 to retain a new set of sample tubes 102. The actuation of the self-locking lug 480 can therefore be automatic, without further action by the user. The action of engaging the self-locking lug 480 with the separator 250 can be the same action as inserting the support 400 into the diagnostic device 300.

[0260] It should be understood that the embodiment of the support 400 can be received in a receiving compartment that does not include a divider 250. In such cases, the hinge assembly 500 can be converted from a tube insertion configuration to a tube retention configuration by actuation of the hinge plate 552 by the user. In some embodiments, regardless of whether the self-locking lug 480 exists, the user converts the hinge plate 552 from the tube insertion configuration to the tube retention configuration. In some embodiments, the self-locking lug 480 is an additional protection to ensure that the hinge assembly 500 has been actuated to the tube retention configuration. In some embodiments, the hinge assembly 500 can be locked by the user or the self-locking lug 480. In some embodiments, the support 400 can be loaded with one or more sample tubes 102 only before being inserted into a diagnostic device 300 such as a receiving compartment with a divider 250. In some embodiments, the support 400 can be loaded with one or more sample tubes 102 before and after being inserted into a diagnostic device 300 such as a receiving compartment with no divider 250.

[0261] Hinge assembly 500 is a mechanical assembly that can slide and pivot, and is used for tube loading and removal when coming out of diagnostic equipment 300. In the tube insertion structure, the hinge assembly has a stable state so that the hinge assembly is locked in an open (open) or loadable structure. Hinge assembly 500 is a mechanical assembly that can slide and pivot, and is used for tube retention when coming out of diagnostic equipment 300 or when in diagnostic equipment 300. In the tube retention structure, the hinge assembly has a stable state so that the hinge assembly is locked in the closed structure. Hinge assembly 500 uses springs and self-closing components to ensure that when placed in diagnostic equipment 300, hinge assembly 500 exists in a closed state. By covering the minimum surface area on the top of the cap 172 of sample tube 102, sample tube 102 is vertically constrained. In the tube retention structure, in some embodiments, the hinge plate 552 of hinge assembly 500 does not touch the top of tube 102. The tube retention configuration allows pipetting while preventing escape of the sample tube 102. The hinge assembly 500 can be considered a spring-locked tube constraint. The hinge assembly 500 is related to sample collection and transport. The hinge assembly 500 is related to machinery and automation.

[0262] Hinge assembly 500 is a mechanism for applying vertical constraints. Hinge assembly 500 can replace other designs, including a series of spring fingers as described herein. When coming out of diagnostic equipment 300, hinge assembly 500 has two states: a tube retention structure, in which sample tube 102 is fixed and cannot escape support 400; and a tube insertion structure, in which a user can remove sample tube 102, insert sample tube 102, and / or remove sample tube 102 from support 400. In some embodiments, when coming out of diagnostic equipment, hinge assembly 500 has only two possible states. In some embodiments, when it is in diagnostic equipment 300, hinge assembly 500 can only operate in a tube retention structure. In some embodiments, when it is inserted into diagnostic equipment 300, hinge assembly 500 is converted into a tube retention structure.

[0263] The hinge assembly 500 works by using two springs 524 that cause the sliding lock 530 and thus the hinge plate 552 to slide laterally within the hinge support 502 and along the hinge pin 522. In the tube retention configuration, the sliding lock 530 prevents rotation of the hinge plate 552. The flange 540 of the sliding lock 530 is within the groove 518. The groove 518 prevents or limits rotation of the flange 540 of the sliding lock 530, and thus prevents or limits rotation of the hinge plate 552 attached to the sliding lock 530. In the tube retention configuration, such as when the bracket 400 is within the diagnostic device 300, the self-locking lug 480 can be actuated to push the flange 568 upward. In the tube retention configuration, such as when the bracket 400 is outside the diagnostic device 300, the self-locking lug 480 may not be actuated. The self-locking lugs 480 are self-closing mechanisms included in the design to ensure that the hinge assembly 500 is always in the tube retaining configuration when placed in the diagnostic device 300 .

[0264] In the tube insertion configuration, the sliding lock 530 is slid relative to the groove 518. The flange 540 of the sliding lock 530 is initially located within the lower opening 516. The flange 540 of the sliding lock 530 is then pivoted outward from the lower opening 516. The sliding lock 530 is pivoted relative to the hinge support 502, and thus the hinge plate 552 attached to the sliding lock 530 is pivoted relative to the hinge support 502. When the spring 524 and the sliding lock 530 have slid so that the flange 540 is aligned with the opening in the surface 504, the flange 540 can be rotated out of the lower opening 516, thereby causing the hinge plate 552 to rotate.

[0265] Hinge assembly 500 has the advantage of being superior to the spring finger design that keeps sample tube in place by contact friction. The spring constant used to produce contact friction has a variable spring constant and can be easily damaged, resulting in the escape of sample tube 102. Hinge assembly 500 does not rely on friction and springs for tube constraint. In the tube retention structure, sample tube 102 does not have a clear path to come out of hinge assembly 500. Sample tube 102 has a hard stopper that prevents it from escaping via interaction with hinge plate 552. Hinge assembly 500 also makes it easier for users to load and remove tubes. When hinge assembly 500 is in the tube insertion structure, the user has two hands that are free from inserting and removing sample tube 102. In some limited situations, support can be reversed, and sample tube 102 can be dumped out from support 400 at the same time. In certain embodiments, a single input force from the user is required to load or unload twelve sample tubes 102, rather than twelve individual input forces required by the user.

[0266] The hinge assembly 500 can prevent or limit vertical movement of the sample tube 102. The hinge assembly 500 can prevent or limit escape of the sample tube 102 from the sample tube holder 408. The hinge assembly 500 can prevent or limit escape of the sample tube 102 from the rack 400 during the instrument workflow. Sample tube escape will cause the operation to be interrupted and require user intervention.

[0267] Hinge assembly 500 can use springs for movement. Hinge assembly 500 does not use springs for movement. Hinge assembly 500 uses less surface area to constrain sample tube 102. Hinge assembly 500 is used to constrain tubes. Hinge assembly 500 uses springs for sliding movement of closing and opening. Hinge assembly 500 uses sliding hinge plate 552 for tube constraint.

[0268] The hinge plate 552 of the hinge assembly 500 is pivoted by the actuation force. In some embodiments, the self-locking lug 480 is a movement that pivots the hinge plate 552 and thus locks the sample tube 102. Prior to the use of the self-locking lug 480, the hinge plate 552 can be in a tube insertion configuration so that the sample tube 102 can be easily loaded into the sample tube holder 408. When in the tube retention configuration, the hinge plate 552 provides vertical constraints for multiple sample tubes 102 in a consistent manner so that the hinge plate 552 can constrain all sample tubes 102 in the sample tube holder 408 at the same time. The overall structure of the hinge plate 552 allows a consistent force to be applied to each sample tube 102. In addition, the pivoting movement of the hinge plate 552 is repeatable so that the same actuation force causes the same pivoting and / or sliding movement of the hinge plate 552. Advantageously, the hinge plate 552 is easily moved between configurations to facilitate loading and unloading of batches of sample tubes during a series of diagnostic tests performed consecutively using the same rack, thereby minimizing user errors and the time to load and unload racks, and increasing pipetting efficiency.

[0269] The above description is intended to illustrate various aspects of the present invention. It is not intended that the embodiments provided herein limit the scope of the present invention. The presently fully described technology will be readily apparent to one of ordinary skill in the art without departing from the spirit or scope of the appended claims.

Claims

1. A device for holding a sample tube, characterized in that Include: a sample tube holder comprising an opening configured to receive a sample tube; and A hinge assembly comprising a hinge plate and a hinge support, wherein the hinge plate is configured to slide and pivot relative to the hinge support between a first structure and a second structure, the hinge plate being positioned to allow the sample tube to be inserted into the opening in the first structure, and the hinge plate being positioned to limit vertical movement of the sample tube within the sample tube holder in the second structure.

2. The device according to claim 1, characterized in that Further included is a sliding lock coupled to the hinge plate.

3. The device according to claim 2, characterized in that The sliding lock is configured to slide and pivot relative to a hinge pin, wherein the hinge pin is coupled to the hinge support.

4. The device according to claim 2, characterized in that Further included is a spring configured to bias the sliding lock against an inner surface of the hinge support.

5. The device according to claim 1, characterized in that Further comprising a reagent housing.

6. The device according to claim 5, characterized in that The sample tube holder and the reagent housing are coupled.

7. The device according to claim 5, characterized in that The hinge support and the reagent housing are coupled.

8. The device according to claim 1, characterized in that The sample tube holder includes a plurality of openings configured to receive a plurality of sample tubes.

9. The device according to claim 1, characterized in that The sample tube holder includes two openings configured to receive the sample tube.

10. The device according to claim 1, characterized in that The opening constrains the sample tube in a horizontal direction when the sample tube is received in the sample tube holder.

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