A colorectal cancer tumor marker detection device

Abstract

The application relates to the marker detection field and specifically relates to a colorectal cancer tumor marker detection device, which comprises a tray, the tray comprises a bottom plate and a plurality of transverse plates and longitudinal plates arranged on the bottom plate, the transverse plates and the longitudinal plates are vertically staggered to form a plurality of rows of placement grooves, and enzyme strips are placed in the placement grooves; a sliding hole is formed in the end of each transverse plate, a sliding groove is formed in the position opposite to each placement groove, and the sliding groove is communicated with the sliding hole and the placement groove; in the application, the designed tray is matched with the enzyme strips, a push rod is arranged in the tray, the push rod is matched with a limiting structure, the enzyme strips are extruded and fixed in the placement grooves, meanwhile, the placement grooves are emptied, the enzyme strips can be conveniently taken out from the placement grooves in an inclined state, and the operation of a detection personnel on a detection process is facilitated.

Description

Technical Field

[0001] This invention relates to the field of biomarker detection, specifically a device for detecting colorectal cancer tumor markers. Background Technology

[0002] The detection of tumor markers for colorectal cancer is of significant clinical importance for early diagnosis, treatment selection, efficacy evaluation, and prognosis. Common colorectal cancer tumor markers include CEA (carcinoembryonic antigen), CA19-9 (carbohydrate antigen 19-9), and CA72-4 (carbohydrate antigen 72-4). These markers can be quantitatively detected using methods such as ELISA, and ELISA plates are the main tool for performing ELISA experiments.

[0003] The specific operating procedure for using an ELISA plate is as follows:

[0004] Coating: Immobilizing specific antibodies in the wells of an ELISA plate or ELISA strip to capture the corresponding antigens in the sample to be tested.

[0005] Blocking: Fill each well with a blocking solution (such as bovine serum albumin or skim milk powder) to reduce nonspecific binding.

[0006] Adding the sample or standard to be tested into the well of the ELISA plate, so that the antigen binds to the coated antibody.

[0007] Washing: Wash each well with washing buffer to remove unbound sample.

[0008] Enzyme-labeled antibody incubation: Add enzyme-labeled secondary antibody to bind with the already bound antigen-antibody complex.

[0009] Washing: Wash again to remove unbound enzyme-labeled antibodies.

[0010] Substrate color development: When a substrate such as tetramethylbenzidine (TMB) is added to the enzyme, a color change occurs under the action of the enzyme.

[0011] To stop the reaction: Add a stop solution (such as sulfuric acid) to stop the enzyme reaction.

[0012] Assay: The absorbance (OD value) of each well is measured at a specific wavelength using an ELISA reader, and the concentration of the analyte in the sample is calculated based on the standard curve.

[0013] In practice, for the detection of small amounts of biomarkers, enzyme-linked immunosorbent assay (ELISA) strips are generally used, such as... Figure 6The shape shown is composed of multiple short tubes strung together to form a strip. To ensure the enzyme label strip remains stable and does not tip over during use, it is generally placed in a special tray with slots for each tube. When using the existing tray with the enzyme label strip, the strip is placed in the tray, and each tube is positioned in its slot. This process can be done with one hand. However, after removing the strip from the tray, one hand holds the dispensing gun, and the other hand grasps the end of the strip. At this point, the entire strip is tilted and moved upwards from the tray. As it moves upwards and detaches from the slots, the lower edge of each tube presses against the inner wall of the slot. This upward movement of the strip causes the tray to move, making it difficult to remove the strip smoothly.

[0014] Therefore, a colorectal cancer tumor marker detection device is proposed to address the above problems. Summary of the Invention

[0015] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0016] The technical solution adopted by the present invention to solve its technical problem is: the colorectal cancer tumor marker detection device of the present invention includes a tray, the tray includes a base plate and a plurality of horizontal plates and vertical plates disposed on the base plate, the horizontal plates and vertical plates are perpendicularly intersected to form multiple rows of placement slots, and enzyme label strips are placed in the placement slots;

[0017] Each of the transverse plates has a sliding hole at its end, and a sliding groove is formed at the part of the sliding hole opposite to each placement groove, with the sliding groove connecting the sliding hole and the placement groove.

[0018] Each of the aforementioned sliding holes is provided with a push rod, the end of which penetrates through the outside of the sliding hole, and a limiting structure is provided on the push rod at a position opposite to the sliding groove;

[0019] Each of the limiting structures includes a connecting part and a pressing part. One end of the connecting part extends along the slide groove into the slide hole and is connected to the push rod. The other end of the connecting part is fixed to the pressing part, which extends upward to the slot opening of the placement groove.

[0020] Preferably, a plurality of sleeves are provided on one side of the upper surface of the base plate, each sleeve being opposite to one end of a sliding hole, and one end of the push rod extending into the sleeve and connected to the sleeve by a spring;

[0021] Multiple notches are provided on the other side of the upper surface of the base plate. Each notch is arranged opposite to the other end of the sliding hole. An elastic plate is provided in the notch. Multiple locking teeth are provided on the upper surface of the elastic plate. A push plate is provided at the other end of each push rod. A locking block is provided on the lower edge of the push plate. The locking block can be locked onto the locking teeth on the upper surface of the elastic plate.

[0022] Preferably, each push rod has multiple annular locking grooves radially formed, and each locking groove is arranged opposite to a placement groove;

[0023] One end of each of the connecting parts is C-shaped, and one end of the connecting part is partially wrapped and can be clamped in the snap-fit ​​groove.

[0024] Preferably, the other end of each of the connecting parts is fixed to the extrusion part by a rubber block, and each extrusion part is arranged in an arc shape, the arc surface of the extrusion part can be extruded onto the outer wall of the enzyme label strip.

[0025] Preferably, the upper surfaces at both ends of the base plate are provided with storage grooves, and support rods are provided in the storage grooves. One end of the support rod is rotatably connected to the storage groove. Insertion holes are provided on the lower surfaces at both ends of the base plate, and the support rods can support and separate the upper and lower base plates.

[0026] Preferably, each of the connecting portions has chamfers on both sides at one end.

[0027] Preferably, the lower surface of the base plate is provided with a plurality of grooves, the grooves are positioned opposite to the placement slots, and each groove contains a plurality of transparent hemispheres, the spherical surface of the hemispheres being fixed to the top surface of the groove.

[0028] Preferably, the lower surface of the hemisphere at the center of each groove is horizontally arranged, and the lower surface of the hemisphere on the outer side of the center of each groove is inclined.

[0029] Preferably, both ends of each sleeve are open.

[0030] Preferably, the outer edge of each of the notches is flared.

[0031] The advantages of this invention are:

[0032] 1. In this invention, the designed tray is used in conjunction with the enzyme-labeled strip. A push rod is set inside the tray, and the push rod, together with the limiting structure, can compress and fix the enzyme-labeled strip in the slot, while also freeing up space in the slot so that the enzyme-labeled strip can be taken out of the slot in an inclined state, which is convenient for the testing personnel to operate in the testing process.

[0033] 2. In this invention, the push rod and limiting structure in the tray are designed to be detachable, which facilitates the cleaning and disinfection of the tray, as well as the cleaning and disinfection of the push rod and limiting structure themselves, ensuring that the tray is thoroughly cleaned so as to prevent residual bacteria or viruses from affecting subsequent testing. Attached Figure Description

[0034] Figure 1 This is a first-view perspective perspective view of the tray in this invention;

[0035] Figure 2This is a second-view perspective perspective view of the tray in this invention;

[0036] Figure 3 This is a top view of the tray in this invention;

[0037] Figure 4 This is a perspective view of the cooperation between the transverse plate and the longitudinal plate in this invention;

[0038] Figure 5 This is a perspective view of the interaction between the elastic plate and the push rod in this invention;

[0039] Figure 6 This is a three-dimensional view of the enzyme label strip in this invention;

[0040] Figure 7 This is a three-dimensional view of the fit between the enzyme label strip and the extrusion part in this invention;

[0041] Figure 8 This is a perspective view of the push rod in this invention;

[0042] Figure 9 This is a perspective view of the interaction between the push rod and the limiting structure in this invention;

[0043] Figure 10 This is a front view of the limiting structure in this invention;

[0044] Figure 11 This is a schematic diagram of the installation of the limiting structure in this invention;

[0045] Figure 12 This is a cross-sectional view of the base plate in this invention;

[0046] Figure 13 This is a schematic diagram of the fit between the push rod and the sleeve in this invention;

[0047] Figure 14 This is a schematic diagram illustrating the engagement of the locking block and locking teeth in this invention;

[0048] Figure 15 This is a bottom view of the tray in this invention;

[0049] Figure 16 This is a schematic diagram of the fit between the hemisphere and the placement groove in this invention.

[0050] In the diagram: 1. Tray; 2. Base plate; 3. Horizontal plate; 4. Vertical plate; 5. Placement slot; 6. Enzyme label strip; 7. Tube body; 8. Sliding hole; 9. Sliding groove; 10. Push rod; 11. Connecting part; 12. Squeezing part; 13. Sleeve; 14. Spring; 15. Elastic plate; 16. Clamping tooth; 17. Push plate; 18. Clamping block; 19. Clamping groove; 20. Rubber block; 21. Storage slot; 22. Support rod; 23. Insertion hole; 24. Chamfer; 25. Groove; 26. Hemisphere; 27. Notch. Detailed Implementation

[0051] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0052] Reference Figure 1 - Figure 7 A colorectal cancer tumor marker detection device includes a tray 1, the tray 1 including a base plate 2 and a plurality of transverse plates 3 and longitudinal plates 4 disposed on the base plate 2, the transverse plates 3 and longitudinal plates 4 are perpendicularly intersected to form multiple rows of placement slots 5, and enzyme labeling strips 6 are placed in the placement slots 5.

[0053] Each of the transverse plates 3 has a sliding hole 8 at its end, and a sliding groove 9 is provided at the part of the sliding hole 8 opposite to each placement groove 5. The sliding groove 9 connects the sliding hole 8 and the placement groove 5.

[0054] Each of the sliding holes 8 is provided with a push rod 10, the end of the push rod 10 passes through the outside of the sliding hole 8, and a limiting structure is provided on the push rod 10 at a position opposite to the sliding groove 9;

[0055] Each of the limiting structures includes a connecting part 11 and a pressing part 12. One end of the connecting part 11 extends along the sliding groove 9 into the sliding hole 8 and is connected to the push rod 10. The other end of the connecting part 11 is fixed to the pressing part 12, which extends upward to the slot opening of the placement groove 5.

[0056] In this embodiment, the colorectal cancer tumor marker detection device also includes an enzyme-linked immunosorbent assay (ELISA) reader. The ELISA strip 6 is placed in the tray 1, and then the tray 1 together with the ELISA strip 6 is placed in the ELISA reader for detection and analysis. The specific process of using the tray 1 with the ELISA strip 6 in this embodiment is as follows:

[0057] Place the enzyme-labeled strip 6, and first pull the pusher 10. Pull the pusher 10 towards the right side of the base plate 2, as shown. Figure 3 As shown, the push rod 10 drives the squeezing part 12 connected to it to move synchronously to the right side of the base plate 2, freeing up space in the placement groove 5. Then, the enzyme labeling strip 6 is placed, and each tube 7 of the enzyme labeling strip 6 is placed in the placement groove 5. Then, the push rod 10 is pushed to the left side of the base plate 2. At this time, the push rod 10 drives the squeezing part 12 on it to squeeze the outer wall of each tube 7 of the enzyme labeling strip 6. At this time, the squeezing part 12 squeezes and limits the entire enzyme labeling strip 6, stabilizing the enzyme labeling strip 6.

[0058] Remove the enzyme label strip 6. Reverse the operation of placing the enzyme label strip 6, pull the push rod 10 to the right side of the base plate 2. At this time, the squeezing part 12 no longer squeezes each tube 7 of the enzyme label strip 6, freeing up space in the placement slot 5. At this time, the space in each placement slot 5 is sufficient for the tube 7 to move smoothly upward from the placement slot 5 in an inclined state. That is, the inner side wall of the placement slot 5 and the side wall of the squeezing part 12 will not hinder the upward movement and detachment of the tube 7. Even when the entire enzyme label strip 6 moves upward from the tray 1 in an inclined state, it can be detached smoothly. For the testing personnel, one hand can hold the liquid dispensing gun and the other hand can easily take the enzyme label strip 6 out of the tray 1, which is convenient for the testing personnel.

[0059] Reference Figure 1 - Figure 7 ,as well as Figure 12 - Figure 14 The base plate 2 has multiple sleeves 13 on one side of its upper surface. Each sleeve 13 is opposite to one end of the sliding hole 8. One end of the push rod 10 extends into the sleeve 13 and is connected to the sleeve 13 by a spring 14.

[0060] Multiple recesses 27 are provided on the other side of the upper surface of the base plate 2. Each recess 27 is arranged opposite to the other end hole of the sliding hole 8. An elastic plate 15 is provided in the recess 27. Multiple locking teeth 16 are provided on the upper surface of the elastic plate 15. A push plate 17 is provided at the other end of each push rod 10. A locking block 18 is provided on the lower edge of the push plate 17. The locking block 18 can be locked onto the locking teeth 16 on the upper surface of the elastic plate 15.

[0061] When placing the enzyme label strip 6, the strip 6 is placed in the placement slot 5. Then, the pusher 10 is pushed, and the pusher plate 17 is set at the other end. The pusher plate 17 drives the pusher 10 to move to the left side of the base plate 2. At the same time, the locking block 18 set at the lower edge of the pusher plate 17 moves along the elastic plate 15. When the pusher 10 moves into place, the locking block 18 locks onto the locking teeth 16. Meanwhile, one end of the pusher 10 is pressed into the sleeve 13 by the spring 14. When it is necessary to remove the enzyme label strip 6 from the tray 1, the tester presses the elastic plate 15 with their finger. The elastic plate 15 tilts downward, and the locking teeth 16 on the elastic plate 15 also disengage from the locking block 18. Under the elastic force of the spring 14 connected to it, the pusher 10 moves to the right side of the base plate 2, and drives the squeezing part 12 to move. The squeezing part 12 no longer squeezes the enzyme label strip 6, further facilitating the tester's operation of the test process.

[0062] Reference Figure 1 - Figure 14 Each push rod 10 has multiple annular locking grooves 19 radially formed, and each locking groove 19 is arranged opposite to the placement groove 5.

[0063] One end of each of the connecting portions 11 is C-shaped, and one end of the connecting portion 11 is partially wrapped and can be clamped in the snap-fit ​​groove 19;

[0064] Considering the cleanliness of the tray 1 and its ease of recycling, after multiple uses, it is necessary to perform cleaning, sterilization, and disinfection operations. During cleaning, it is necessary to thoroughly clean every part inside the tray 1. Therefore, the push rod 10 and the limiting structure are designed to be detachable. That is, one end of the connecting part 11 is C-shaped and can extend along the slide groove 9 into the slide hole 8 and be locked in the locking groove 19 on the push rod 10. By forcefully pulling the limiting structure, the connecting part 11 is disengaged from the push rod 10, and then the push rod 10 is removed from the slide hole 8. Each placement slot 5, slide groove 9, and slide hole 8 are then immersed for cleaning and disinfection. At the same time, the removed slide rod and limiting structure are also thoroughly cleaned and disinfected to ensure that the tray 1 is thoroughly cleaned so as to prevent residual bacteria or viruses from affecting subsequent testing.

[0065] After cleaning, disinfection and drying, assemble. First, install each push rod 10 into the sliding hole 8, then install the limiting structure. Align the C-shaped part of the connecting part 11 with the sliding groove 9 and press it firmly into the snap-fit ​​groove 19 on the push rod 10.

[0066] Reference Figure 10 The other end of each of the connecting parts 11 is fixed to the extrusion part 12 by a rubber block 20, and each extrusion part 12 is arranged in an arc shape, and the arc surface of the extrusion part 12 can be extruded onto the outer wall of the enzyme label strip 6.

[0067] Considering that there are various sizes of enzyme labeling strips 6, that is, the diameter of the tube body 7 of enzyme labeling strips 6 is different, and there are cases where different sizes of enzyme labeling strips 6 are placed in the same row of placement slots 5, rubber blocks 20 are provided in the connecting part 11 and the squeezing part 12. By utilizing the elasticity of the rubber blocks 20, the squeezing parts 12 in the same row can effectively squeeze the tube bodies 7 of different sizes, so as to squeeze and fix the enzyme labeling strips 6 of different sizes of tube bodies 7.

[0068] Reference Figure 1 - Figure 3 ,as well as Figure 12 The upper surfaces of both ends of the base plate 2 are provided with storage grooves 21, and support rods 22 are provided in the storage grooves 21. One end of the support rods 22 is rotatably connected in the storage grooves 21. Insertion holes 23 are provided on the lower surfaces of both ends of the base plate 2. The support rods 22 can support and separate the upper and lower base plates 2.

[0069] The support rod 22 is used to separate the upper and lower trays 1. The support rod 22 is rotated to be perpendicular to the base plate 2. The upper end of the support rod 22 is inserted into the insertion hole 23 on the lower surface of the upper tray 1, which facilitates the stacking and detection of a large number of markers. When the support is not needed, the support rod 22 can be rotated into the storage slot 21 to avoid the support rod 22 from obstructing the detection operation.

[0070] Reference Figure 9 - Figure 11 Each of the connecting parts 11 has chamfers 24 on both sides at one end;

[0071] Chamfers 24 are provided on both sides of one end of the connecting part 11, such as Figure 11 As shown, when installing the limiting structure, the limiting structure is placed in an inclined state so that one end of the connecting part 11 is quickly aligned with the snap-fit ​​groove 19. Then, the connecting part 11 is pressed with force, and the limiting structure and the push rod 10 are rotated to a vertical state. This facilitates the installation and connection of each limiting structure and the push rod 10, and does not require brute force to install the limiting structure.

[0072] Reference Figure 15 - Figure 16 The lower surface of the base plate 2 is provided with a plurality of grooves 25, which are arranged one-to-one with the placement grooves 5. Each groove 25 contains a plurality of transparent hemispheres 26, and the spherical surface of the hemispheres 26 is fixed to the top surface of the groove 25.

[0073] The groove 25 is designed to protect the hemisphere 26, which is used to focus the light beam emitted by the microplate reader, allowing the beam to concentrate on the tube 7 inside the placement groove 5, thus improving the utilization of light. The light beam emitted by the microplate reader's light source becomes a monochromatic beam after passing through a filter. This monochromatic beam is focused by the hemisphere 26 onto the sample to be tested inside the tube 7. After a portion is absorbed, the light reaches the photodetector. The photodetector converts the intensity of the light signal projected onto it into the magnitude of the electrical signal. After analog signal processing such as analog-to-digital conversion, the electrical signal is sent to the microprocessor for data processing and calculation. Finally, the test result is output by the result output section.

[0074] Reference Figure 16 The lower surface of the hemisphere 26 at the center of each groove 25 is horizontally arranged, and the lower surface of the hemisphere 26 on the outer side of the center of each groove 25 is inclined.

[0075] The inclined arrangement of the outer hemisphere 26 at the center of the groove 25 allows the light beam scattered outward from the light source to be refracted onto the surface of the tube 7, further improving the utilization rate of the light beam.

[0076] Reference Figure 13 Each of the sleeves 13 is open at both ends; the open design of the sleeves 13 allows air to flow through the sleeves 13 during subsequent cleaning, which helps the tray 1 to dry thoroughly.

[0077] Reference Figure 5Each of the recesses 27 has an flared outer opening; the flared design of the recesses 27 facilitates the pressing of the elastic plate 15 by the fingers of the testing personnel.

[0078] Working principle: In this embodiment, the colorectal cancer tumor marker detection device also includes an enzyme-linked immunosorbent assay (ELISA) reader. The ELISA strip 6 is placed in the tray 1, and then the tray 1 together with the ELISA strip 6 is placed in the ELISA reader for detection and analysis. The specific process of using the tray 1 with the ELISA strip 6 in this embodiment is as follows:

[0079] Place the enzyme-labeled strip 6, and first pull the pusher 10. Pull the pusher 10 towards the right side of the base plate 2, as shown. Figure 3 As shown, the push rod 10 drives the squeezing part 12 connected to it to move synchronously to the right side of the base plate 2, freeing up space in the placement groove 5. Then, the enzyme labeling strip 6 is placed, and each tube 7 of the enzyme labeling strip 6 is placed in the placement groove 5. Then, the push rod 10 is pushed to the left side of the base plate 2. At this time, the push rod 10 drives the squeezing part 12 on it to squeeze the outer wall of each tube 7 of the enzyme labeling strip 6. At this time, the squeezing part 12 squeezes and limits the entire enzyme labeling strip 6, stabilizing the enzyme labeling strip 6.

[0080] Remove the enzyme label strip 6. In the opposite direction to placing the enzyme label strip 6, pull the push rod 10 to the right side of the base plate 2. At this time, the squeezing part 12 no longer squeezes each tube 7 of the enzyme label strip 6, freeing up space in the placement slot 5. At this time, the space in each placement slot 5 is sufficient for the tube 7 to move smoothly upward from the placement slot 5 in an inclined state. That is, the inner side wall of the placement slot 5 and the side wall of the squeezing part 12 will not hinder the upward movement and detachment of the tube 7. Even when the entire enzyme label strip 6 moves upward from the tray 1 in an inclined state, it can be detached smoothly. For the testing personnel, one hand can hold the liquid dispensing gun and the other hand can easily take the enzyme label strip 6 out of the tray 1, which is convenient for the testing personnel.

[0081] When placing the enzyme label strip 6, the strip 6 is placed in the placement slot 5. Then, the pusher 10 is pushed, and the other end of the pusher 10 is set with a pusher plate 17. The pusher plate 17 drives the pusher 10 to move to the left side of the base plate 2. At the same time, the locking block 18 set on the lower edge of the pusher plate 17 moves along the elastic plate 15. When the pusher 10 moves into place, the locking block 18 locks onto the locking teeth 16. Meanwhile, one end of the pusher 10 is pressed into the sleeve 13 by the spring 14. When it is necessary to remove the enzyme label strip 6 from the tray 1, the tester presses the elastic plate 15 with their finger. The elastic plate 15 tilts downward, and the locking teeth 16 on the elastic plate 15 also disengage from the locking block 18. Under the elastic force of the spring 14 connected to it, the pusher 10 moves to the right side of the base plate 2 and drives the squeezing part 12 to move. The squeezing part 12 no longer squeezes the enzyme label strip 6, further facilitating the tester's operation of the test process.

[0082] Considering the cleanliness of the tray 1 and its ease of recycling, after multiple uses, it is necessary to perform cleaning, sterilization, and disinfection operations. During cleaning, it is necessary to thoroughly clean every part inside the tray 1. Therefore, the push rod 10 and the limiting structure are designed to be detachable. That is, one end of the connecting part 11 is C-shaped and can extend along the slide groove 9 into the slide hole 8 and be locked in the locking groove 19 on the push rod 10. By forcefully pulling the limiting structure, the connecting part 11 is disengaged from the push rod 10, and then the push rod 10 is removed from the slide hole 8. Each placement slot 5, slide groove 9, and slide hole 8 are then immersed for cleaning and disinfection. At the same time, the removed slide rod and limiting structure are also thoroughly cleaned and disinfected to ensure that the tray 1 is thoroughly cleaned so as to prevent residual bacteria or viruses from affecting subsequent testing.

[0083] After cleaning, disinfection and drying, assemble. First, install each push rod 10 into the sliding hole 8, then install the limiting structure. Align the C-shaped part of the connecting part 11 with the sliding groove 9 and press it firmly into the snap-fit ​​groove 19 on the push rod 10.

[0084] Considering that there are various sizes of enzyme labeling strips 6, that is, the diameter of the tube body 7 of enzyme labeling strips 6 is different, and there are cases where enzyme labeling strips 6 of different sizes are placed in the same row of placement slots 5, rubber blocks 20 are provided in the connecting part 11 and the squeezing part 12. By utilizing the elasticity of the rubber blocks 20, the squeezing parts 12 in the same row can effectively squeeze the tube bodies 7 of different sizes, so as to squeeze and fix the enzyme labeling strips 6 of different sizes of tube bodies 7.

[0085] The support rod 22 is used to separate the upper and lower trays 1. The support rod 22 is rotated to be perpendicular to the base plate 2. The upper end of the support rod 22 is inserted into the insertion hole 23 on the lower surface of the upper tray 1, which facilitates the detection and stacking of a large number of markers. When the support is not needed, the support rod 22 can be rotated into the storage slot 21 to avoid the support rod 22 from obstructing the detection operation.

[0086] Chamfers 24 are provided on both sides of one end of the connecting part 11, such as Figure 11 As shown, when installing the limiting structure, the limiting structure is placed in an inclined state so that one end of the connecting part 11 is quickly aligned with the snap-fit ​​groove 19. Then, the connecting part 11 is pressed with force, and the limiting structure and the push rod 10 are rotated to a vertical state. This facilitates the installation and connection of each limiting structure and the push rod 10, and does not require brute force to install the limiting structure.

[0087] The groove 25 is designed to protect the hemisphere 26, which is used to focus the light beam emitted by the microplate reader, allowing the light beam to be concentrated onto the tube 7 inside the placement groove 5, thus improving the utilization of light. The light beam emitted by the microplate reader's light source lamp becomes a monochromatic light after passing through the filter. This monochromatic light beam is focused by the hemisphere 26 onto the sample to be tested inside the tube 7. After a portion is absorbed, it reaches the photodetector. The photodetector converts the intensity of the light signal projected onto it into the magnitude of the electrical signal. After analog signal processing such as analog-to-digital conversion, the electrical signal is sent to the microprocessor for data processing and calculation. Finally, the test result is output by the result output section.

[0088] The inclined arrangement of the outer hemisphere 26 at the center of the groove 25 allows the light beam scattered outward from the light source to be refracted onto the surface of the tube 7, further improving the utilization rate of the light beam.

[0089] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for detecting colorectal cancer tumor markers, characterized in that: The tray (1) includes a base plate (2) and multiple horizontal plates (3) and vertical plates (4) disposed on the base plate (2). The horizontal plates (3) and vertical plates (4) are perpendicularly intersected to form multiple rows of placement slots (5). Enzyme label strips (6) are placed in the placement slots (5). Each of the transverse plates (3) has a sliding hole (8) at its end, and a sliding groove (9) is provided at the part of the sliding hole (8) opposite to each placement groove (5). The sliding groove (9) connects the sliding hole (8) and the placement groove (5). Each of the sliding holes (8) is provided with a push rod (10), the end of the push rod (10) passes through the outside of the sliding hole (8), and a limiting structure is provided on the push rod (10) relative to the sliding groove (9); Each of the limiting structures includes a connecting part (11) and a pressing part (12). One end of the connecting part (11) extends along the slide groove (9) into the slide hole (8) and is connected to the push rod (10). The other end of the connecting part (11) is fixed to the pressing part (12). The pressing part (12) extends upward to the slot opening of the placement groove (5). The base plate (2) has multiple sleeves (13) on one side of its upper surface. Each sleeve (13) is opposite to one end of the sliding hole (8), and one end of the push rod (10) extends into the sleeve (13) and is connected to the sleeve (13) by a spring (14). Multiple recesses (27) are provided on the other side of the upper surface of the base plate (2). Each recess (27) is arranged opposite to the other end hole of the sliding hole (8). An elastic plate (15) is provided in the recess (27). Multiple locking teeth (16) are provided on the upper surface of the elastic plate (15). Each push rod (10) is provided with a push plate (17) at the other end. A locking block (18) is provided on the lower edge of the push plate (17). The locking block (18) can be locked onto the locking teeth (16) on the upper surface of the elastic plate (15).

2. The colorectal cancer tumor marker detection device according to claim 1, characterized in that: Each push rod (10) has multiple annular snap-fit ​​grooves (19) radially formed, and each snap-fit ​​groove (19) is arranged opposite to the placement groove (5); One end of each of the connecting parts (11) is C-shaped, and one end of the connecting part (11) is partially wrapped and can be clamped in the snap-fit ​​groove (19).

3. The colorectal cancer tumor marker detection device according to claim 2, characterized in that: The other end of each of the connecting parts (11) is fixed to the extrusion part (12) by a rubber block (20), and each extrusion part (12) is arranged in an arc shape, and the arc surface of the extrusion part (12) can be extruded onto the outer wall of the enzyme label strip (6).

4. The colorectal cancer tumor marker detection device according to claim 1, characterized in that: The upper surfaces of both ends of the base plate (2) are provided with storage grooves (21), and support rods (22) are provided in the storage grooves (21). One end of the support rods (22) is rotatably connected in the storage grooves (21). Insertion holes (23) are provided on the lower surfaces of both ends of the base plate (2). The support rods (22) can support and separate the upper and lower base plates (2).

5. The colorectal cancer tumor marker detection device according to claim 3, characterized in that: Each of the connecting parts (11) has chamfers (24) on both sides of one end.

6. The colorectal cancer tumor marker detection device according to claim 5, characterized in that: The lower surface of the base plate (2) is provided with multiple grooves (25), and the grooves (25) are positioned opposite to the placement slots (5). Each groove (25) contains multiple transparent hemispheres (26), and the spherical surface of the hemispheres (26) is fixed to the top surface of the groove (25).

7. The colorectal cancer tumor marker detection device according to claim 6, characterized in that: The lower surface of the hemisphere (26) at the center of each groove (25) is horizontally arranged, and the lower surface of the hemisphere (26) on the outer side of the center of each groove (25) is inclined.

8. The colorectal cancer tumor marker detection device according to claim 2, characterized in that: Both ends of each of the sleeves (13) are open.

9. The colorectal cancer tumor marker detection device according to claim 1, characterized in that: Each of the notches (27) has an flared outer opening.

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