A tumor specimen sealing device and method

Abstract

The application relates to the technical field of specimen preservation equipment, in particular to a tumor specimen sealing device. The device comprises a cold storage tank, a plurality of sealing chambers are embedded at the port of the cold storage tank, a plurality of shockproof clamping plates are arranged in an annular array in the sealing chambers, a pump I corresponding to each sealing chamber is arranged on the inner bottom wall of the cold storage tank, the outlet end of the pump I is communicated with an electromagnetic reversing valve, the outlet of the electromagnetic reversing valve is respectively communicated with a pipe I and a pipe II, the upper end of the pipe I is inserted into the sealing chamber, the bottom end of the sealing chamber is communicated with a pump II, the upper end of the pipe II is communicated with a shunt pipe corresponding to each clamping plate, the other end of the shunt pipe is penetrated through the bottom wall of the sealing chamber after being annularly arranged in the corresponding clamping plate and then extended out. The scheme is characterized in that liquid nitrogen and the clamping plates are used for cold exchange, slow cooling to a set temperature is carried out, then the liquid nitrogen is used in a liquid nitrogen covering and immersion mode to rapidly cool to the liquid nitrogen temperature. The scheme can make the extracellular crystals and the intracellular solution reach vitrification, reduce the damage of cooling to the tumor tissue, and ensure the activity of the tumor tissue cells.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of specimen preservation equipment, and particularly relates to a tumor specimen sealing device. BACKGROUND

[0002] Tumor is a major disease that seriously threatens human life and health. In order to reduce the incidence and mortality of tumor, researchers in various countries are conducting unremitting research, and preservation of tumor tissues removed by surgery, establishment of a tumor tissue library, and then research and utilization, will greatly promote the development of anti-tumor research. If the tumor specimen has activity under low-temperature preservation, and various biochemical physical indicators are equivalent to fresh tissues, the reliability of the specimen will be greatly improved. If the tumor tissue with activity is cultured, more parallel samples can be obtained, which will greatly alleviate the problem of sample shortage; if the tumor tissue cells with activity are implanted into experimental animals, they can be used for tumor immunotherapy, therefore, it is of great significance to study the low-temperature preservation technology of tumor tissues.

[0003] Most of the metabolic activities in living organisms are catalyzed by enzymes, and low temperature can inhibit the activity of enzymes, thereby inhibiting the biochemical activities of living organisms, therefore, theoretically, tumor tissues can be preserved for a long time at low temperature. However, during the cooling process, the tumor tissues are easily damaged by factors such as freezing and thawing of the solution.

[0004] At present, the generally accepted reason for cell damage during cooling is the "two-factor hypothesis": one is the formation of ice crystals in cells caused by rapid cooling, which causes physical damage to cells; the other is the damage of solutes caused by slow cooling. Therefore, it can be known that rapid cooling and continuous slow cooling will cause damage to tumor tissues and affect the activity, and then affect the research reliability.

[0005] Therefore, the present application provides a tumor specimen sealing device, which can minimize the damage of cooling to tumor tissues while preserving tumor tissue specimens at low temperature.

[0006] CONTENT OF THE APPLICATION

[0007] The purpose of the present application is to solve the problems in the prior art and provide a tumor specimen sealing device.

[0008] In order to achieve the above purpose, the present application adopts the following technical scheme:

[0009] The utility model provides a tumor specimen sealing device, including cold storage tank and tank cover with heat insulation layer, the cold storage tank port is equipped with the heat insulation sealing plate, and a plurality of sealing chambers are embedded on the sealing plate, a plurality of shockproof clamping plates are arranged in the annular array in the sealing chamber, the sealing chamber port is equipped with the cover, the bottom wall of the cold storage tank is equipped with pump no.

[0010] Preferably, the cold storage tank is provided with a sandwich layer, the lower end of the sandwich layer is provided with a pump three in communication with the cold storage tank, the upper end of the sandwich layer is provided with an air pipe in communication with the sealing chamber, a pressure relief valve is arranged in the air pipe, the outlet of the electromagnetic reversing valve is further connected with a pipe three, the pipe three is inserted into the sealing chamber and is provided with an atomizing nozzle at the port.

[0011] Preferably, the bottom wall of the sealing chamber is uniformly provided with a plurality of elastic supporting blocks.

[0012] Preferably, a plurality of vertical gaps are arranged on the side of the clamping plate facing the center of the sealing chamber.

[0013] Preferably, a plurality of heat-conducting plates are embedded in the inner wall of the cold storage tank.

[0014] Preferably, the bottom end of the cover is provided with a horn cover.

[0015] Preferably, a plurality of elastic blocks are uniformly arranged between the clamping plate and the inner wall of the sealing chamber.

[0016] Preferably, the tank cover is provided with an opening corresponding to the cover.

[0017] Preferably, a filler pipe is embedded in the center of the tank cover and extends into the bottom end of the cold storage tank.

[0018] The sealing method of the tumor specimen sealing device comprises the following steps:

[0019] S1: precooling;

[0020] S2: specimen is put into the sealing;

[0021] S3: slowly cooling to a certain temperature;

[0022] S4: rapid cooling to liquid nitrogen temperature.

[0023] Compared with the prior art, the tumor specimen sealing device provided by the utility model has the following beneficial effects:

[0024] 1. In operation, pump one first draws liquid nitrogen into pipe two. The liquid nitrogen then flows through pipe two into the distribution pipe, and then flows from the end of the distribution pipe into the cold storage tank. During this process, the low temperature of the liquid nitrogen is transferred to the clamping plate, which in turn transfers the low temperature to the cold storage tube, thus slowly cooling the internal environment of the cold storage tube. Once the temperature inside the sealed chamber reaches the set temperature, pump one injects liquid nitrogen into pipe one, which then enters the sealed chamber. The liquid nitrogen accumulates in the sealed chamber, covering the cold storage tube. The cold storage tube is largely immersed in liquid nitrogen, cooling it to a temperature close to or the same as that of the liquid nitrogen, thus achieving rapid cooling. This scheme first utilizes liquid nitrogen for cold exchange with the clamping plate, and then for contact cooling with the cold storage tube. The tumor tissue cells are first slowly cooled to the set temperature in a solution containing cryoprotectant; then, they are rapidly cooled to the liquid nitrogen temperature through immersion in liquid nitrogen. This process ultimately achieves partial crystallization vitrification, meaning that while there is crystallization outside the cell, the intracellular solution becomes vitrified. This can significantly reduce the damage to tumor tissue caused by cooling and ensure the viability of tumor cells during cryopreservation.

[0025] 2. After the cold storage tube is submerged in liquid nitrogen over a large area, pump two starts and maintains the same delivery efficiency as pump one, so that the amount of liquid nitrogen injected into the sealed chamber by pump one and the amount of liquid nitrogen pumped away by pump two remain the same, so that liquid nitrogen continues to circulate and ensures good cooling effect.

[0026] 3. When the cold storage tube is removed, pump two continues to run to completely remove the liquid nitrogen from the sealed chamber. Opening the cap at this time will reduce the amount of liquid nitrogen that will vaporize and overflow due to contact with the outside environment, thus reducing waste.

[0027] 4. Each cold storage tube is stored individually, thus avoiding cross-infection between tissue samples; and the entire process is carried out under sterile conditions, thus avoiding contamination by exogenous microorganisms.

[0028] 5. The sealed chamber isolates liquid nitrogen from the external environment, and the coordinated use of pipes one, two, and three allows the liquid nitrogen to flow within and out of the sealed chamber. When opened, direct contact between the liquid nitrogen and the external environment is avoided, thus reducing liquid nitrogen contamination and preventing any impact on the aseptic storage conditions of the cold storage tubes.

[0029] Other advantages, objectives and features of this application will be set forth in part in the description which follows; and in part will be apparent to those skilled in the art upon examination of the following description; or may be learned from practice of this application. Attached Figure Description

[0030] Figure 1 This is a three-dimensional schematic diagram of this application.

[0031] Figure 2 This is a top view of the present application.

[0032] Figure 3 A-A cross-sectional view of the tank cover of the present application. Figure 2 A-A cross-sectional view of the tank cover of the present application.

[0033] Figure 4 A-A cross-sectional view of the tank cover of the present application.

[0034] Figure 5 A-A cross-sectional view of the tank cover of the present application. Figure 3 A-A cross-sectional view of the tank cover of the present application.

[0035] Figure 6 A-A cross-sectional view of the tank cover of the present application.

[0036] Figure 7 A-A cross-sectional view of the tank cover of the present application. Figure 5 A-A cross-sectional view of the tank cover of the present application.

[0037] Figure 8 A-A cross-sectional view of the tank cover of the present application.

[0038] Figure 9 A-A cross-sectional view of the tank cover of the present application.

[0039] Figure 10 A-A cross-sectional view of the tank cover of the present application. Figure 9 A-A cross-sectional view of the tank cover of the present application.

[0040] Figure: 1, cold storage tank; 2, sealing plate; 3, sealing chamber; 4, tank cover; 5, cover; 6, clamp plate; 7, elastic block; 8, elastic support block; 9, interlayer; 10, pump one; 11, pipe one; 12, pipe two; 13, pipe three; 14, pump two; 15, pump three; 16, gas pipe; 17, horn cover; 18, atomizing nozzle; 19, shunt pipe; 20, electromagnetic reversing valve; 21, heat conducting plate; 22, filler pipe; 23, compartment; 24, pressure regulating pipe; 25, air cylinder; 26, lower pressing spring; 27, pressing claw; 28, buckling claw; 29, top rod; 30, pressure regulating channel; 31, sealing ball; 32, nitrogen spring; 33, vacuum pump; 34, top block. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Figures 1-10 It is obvious that the described embodiments are only some of the embodiments of the present application, but not all of the embodiments of the present application.

[0042] In order to solve the problem of tumor tissue damage during low temperature storage of tumor tissue in the prior art, the embodiment provides a tumor specimen sealing device, which comprises a cold storage tank 1 with a heat insulation layer and a tank cover 4, the cold storage tank 1 is provided with a heat insulation sealing plate 2 at the port, a plurality of sealing chambers 3 are embedded on the sealing plate 2, a plurality of shockproof clamping plates 6 are arranged in an annular array in the sealing chamber 3, a sealing cover 5 is arranged at the port of the sealing chamber 3, a pump I 10 corresponding to the sealing chamber 3 is arranged on the inner bottom wall of the cold storage tank 1, the outlet end of the pump I 10 is communicated with an electromagnetic reversing valve 20, the outlet of the electromagnetic reversing valve 20 is respectively communicated with a pipe I 11 and a pipe II 12, the upper end of the pipe I 11 is inserted into the sealing chamber 3, the bottom end of the sealing chamber 3 is communicated with a pump II 14, the upper end of the pipe II 12 is communicated with a shunt pipe 19 corresponding to the clamping plate 6, and the other end of the shunt pipe 19 is arranged to surround the corresponding clamping plate 6 and then extend out of the bottom wall of the sealing chamber 3.

[0043] Principle details of the embodiment:

[0044] The tumor specimen sealing device comprises a cold storage tank 1 and a tank cover 4. The periphery of the cold storage tank 1 and the tank cover 4 is a flange structure and is fastened with self-locking bolts and nuts, so that the cold storage tank 1 and the tank cover 4 can be separated for internal maintenance and replacement. The cold storage tank 1 is filled with sterile liquid nitrogen.

[0045] The cold storage tank 1 port is embedded with a sealing plate 2, and the sealing plate 2 has a plurality of through holes arranged in an annular array on the side. The cold storage tank 1 port is provided with a threaded hole corresponding to the through hole. The sealing plate 2 is fastened and installed at the cold storage tank 1 port by screwing a screw through the through hole and into the threaded hole. A sealing strip is arranged at the contact position between the sealing plate 2 and the cold storage tank 1 port to increase the airtightness and prevent cold gas leakage. The sealing plate 2 has a plurality of mounting holes arranged in an annular array, and a plurality of sealing chambers 3 are fastened and installed in the mounting holes. The sealing chamber 3 port is provided with a cover 5, and the cover 5 and the sealing chamber 3 periphery are also flange structures and are fastened and connected by a screw. A sealing gasket is arranged at the contact position between the cover 5 and the sealing chamber 3 to prevent air leakage. The sealing chamber 3 has a plurality of clamping plates 6 arranged in an annular array. The clamping plate 6 has a shockproof function and is used to clamp a storage container such as a sample tube containing tumor tissue, and has a shockproof effect. The clamping plate 6 has good cold and heat transfer properties, such as copper 401, aluminum 237, iron 80, and tin 67. The cold storage tank 1 inner bottom wall is provided with a pump one 10 corresponding to the sealing chamber 3. The pump one 10 has an inlet end and an outlet end. The inlet end of the pump one 10 is close to the bottom wall of the cold storage tank 1 and is used to suck liquid nitrogen. The outlet end of the pump one 10 is communicated with an electromagnetic reversing valve 20. In this embodiment, the electromagnetic reversing valve 20 is a two-way valve with one inlet and two non-communicating outlets. The two outlets of the electromagnetic reversing valve 20 are respectively communicated with a pipe one 11 and a pipe two 12. The upper end of the pipe one 11 is inserted into the sealing chamber 3, and the bottom end of the sealing chamber 3 is communicated with a pump two 14 located in the cold storage tank 1. The outlet end of the pump two 14 extends into the cold storage tank 1. The upper end of the pipe two 12 is communicated with a shunt pipe 19 corresponding to the clamping plate 6. The other end of the shunt pipe 19 is inserted into the corresponding clamping plate 6 from the bottom end, and then winds around in a zigzag shape in the clamping plate 6, and then extends out from the other side of the bottom end of the clamping plate 6 and extends out from the bottom wall of the sealing chamber 3.

[0046] In this embodiment, the cold storage tank 1 and the sealing chamber 3 are provided with low-temperature-resistant temperature sensors, such as Cernox temperature sensors, which can accurately measure the temperature of the environment in a low-temperature environment and monitor the storage environment temperature of the specimen in a timely manner.

[0047] In this embodiment, the cold storage tank 1, the sealing plate 2 of the tank cover 4, the sealing chamber 3, and the cover 5 are all provided with a heat preservation and insulation layer to prevent cold gas leakage and ensure the stability of the tumor tissue cold storage environment.

[0048] In this embodiment, in order to prevent the pump one 10 and the pump two 14 from being affected by low temperature and affecting their normal operation, two structure forms are provided to isolate the low temperature:

[0049] First, a plurality of pump one 10 and pump two 14 are externally irradiated with a temperature isolation cover. The temperature isolation cover is provided with a heat insulation layer. The inlet and outlet of the pump one 10 and the pump two 14 respectively penetrate the temperature isolation cover, thereby achieving a certain low-temperature freezing prevention function.

[0050] Second, refer to the attachedFigure 8 As shown, a partition 23 is arranged at the bottom end of the cold storage tank 1, and a heat insulation layer is arranged in the partition 23. The pump 1 and the pump 2 are arranged in the partition 23, thereby being isolated from the liquid nitrogen, and further having a certain low-temperature freezing prevention function.

[0051] In the embodiment, the whole process and each device are sterilized, and in the non-use state, the sterilization is performed at a regular time.

[0052] According to the above technical scheme:

[0053] In use, the collected specimen is first fixed in a proper fixing liquid to fix the structure and morphology of the tissue cells. The fixed tissue specimen is buried in a wax block. Then, the buried specimen is cut into a thin slice. Then, the specimen support is put into a cold storage tube containing a cryoprotective agent, and a cap is quickly screwed on (before use, the cap of the cold storage tube is unscrewed, the upper part of the cold storage tube is clamped by a clamp, and is pre-cooled in a liquid nitrogen cup). Then, the cold storage tube is put into the sealed chamber 3 and is clamped by the clamp plate 6. The cover 5 is covered.

[0054] The pump 1 corresponding to the sealed chamber 3 is started. The pump 1 first draws the liquid nitrogen into the pipe 2. The liquid nitrogen enters the shunt pipe 19 along the pipe 2, and then flows into the cold storage tank 1 from the end of the shunt pipe 19. In this process, the low temperature of the liquid nitrogen is transferred to the clamp plate 6, and the clamp plate 6 with good cold conduction performance further transfers the low temperature to the cold storage tube, thereby slowly cooling the internal environment of the cold storage tube. When the temperature sensor in the sealed chamber 3 detects that the cooling tube is cooled to a set temperature, the electromagnetic reversing valve 20 is reversed. At this time, the pump 1 injects the liquid nitrogen into the pipe 1 and enters the sealed chamber 3. The liquid nitrogen accumulates in the sealed chamber 3 and covers the cold storage tube. The cold storage tube is immersed in a large area by the liquid nitrogen and is cooled to a temperature close to or the same as the liquid nitrogen, thereby achieving the purpose of rapid cooling. After the cold storage tube is immersed in a large area by the liquid nitrogen, the pump 2 is started and has the same conveying efficiency as the pump 1. The amount of liquid nitrogen injected by the pump 1 in the sealed chamber 3 and the amount of liquid nitrogen drawn away by the pump 2 are kept the same, so that the liquid nitrogen is continuously circulated to ensure good cold conduction effect.

[0055] When the cold storage tube is taken out, the electromagnetic reversing valve 20 makes the pump 1 communicate with the pipe 2, circulates the liquid nitrogen in the shunt pipe 19, and the clamp plate 6 continues to cool in contact with the cold storage tube; and the pump 2 continuously runs to completely separate the liquid nitrogen in the sealed chamber 3. At this time, the cover 5 is opened, and the cold storage tube is taken out, which will reduce the amount of liquid nitrogen gasified and overflowed due to contact with the outside world, and reduce waste.

[0056] Each cold storage tube stores individually, thereby avoiding cross infection between tissue samples; and the whole process is carried out in a sterile condition, thereby avoiding contamination by external microorganisms.

[0057] The liquid nitrogen is isolated from the external environment by the sealed chamber 3, and the liquid nitrogen flows in and out of the sealed chamber 3 through the cooperation of pipe one 11, pipe two 12 and pipe three 13. When the device is opened, the direct contact between the liquid nitrogen and the external environment is avoided, thereby reducing the pollution of the liquid nitrogen and avoiding the influence on the sterile storage conditions of the cold storage tube.

[0058] In summary, the present scheme first utilizes liquid nitrogen to perform cold exchange with the clamp plate 6, and then performs contact cooling with the cold storage tube. The tumor tissue cells are first slowly cooled to a set temperature in a solution containing a cryoprotective agent; and then the liquid nitrogen is used to quickly cool to the liquid nitrogen temperature in a wrapping and immersion manner. The ultimate realization of this process is partial crystallization and vitrification, that is, the solution in the cells is vitrified while the outside of the cells is crystallized, which can more greatly reduce the damage of cooling to the tumor tissue and ensure the activity of the tumor tissue cells in cold storage.

[0059] In the further embodiment of the present scheme, different cells require different optimal cooling rates due to the differences in cell structure, size and properties. Although the running power of pump one 10 and pump two 14 can be adjusted to adjust the delivery efficiency of the liquid nitrogen and then adjust the cooling efficiency in the slow cooling stage, such a way of controlling the flow rate of the liquid nitrogen is too slow and inefficient. Therefore, in the present embodiment:

[0060] The cold storage tank 1 is provided with a clamping layer 9, the lower end of the clamping layer 9 is provided with a pump three 15 communicating with the cold storage tank 1, and the upper end of the clamping layer 9 is provided with an air pipe 16 communicating with the sealed chamber 3, the air pipe 16 is provided with a pressure relief valve, the outlet of the electromagnetic reversing valve 20 is also connected with the pipe three 13, and the pipe three 13 is inserted into the sealed chamber 3 and provided with an atomizing nozzle 18 at the port.

[0061] The principle details of the present embodiment are as follows:

[0062] In the present embodiment, the electromagnetic reversing valve 20 is a three-way valve, that is, one inlet and three outlets. The inlet of the electromagnetic reversing valve 20 communicates with the outlet end of the pump one 10, and the three outlets of the electromagnetic reversing valve 20 are connected with the pipe one 11, the pipe two 12 and the pipe three 13 respectively. The pipe one 11 is directly inserted into the sealed chamber 3, the pipe two 12 is connected with the shunt pipe 19, the pipe three 13 is also inserted into the sealed chamber 3 and provided with the atomizing nozzle 18 at the port of the pipe three 13, the atomizing nozzle 18 is a liquid nitrogen atomizer for atomizing the liquid nitrogen. The air pipe 16 is inserted into the clamping layer 9 at the side wall of the sealed chamber 3, and the air pipe 16 is provided with a pressure relief valve. The clamping layer 9 is provided on the side wall of the cold storage tank 1, and the clamping layer 9 is also provided with a heat preservation and insulation layer to avoid cold leakage. The air pipe 16 is inserted into the clamping layer 9 at the end. The bottom end of the clamping layer 9 is provided with the pump three 15, and the pump three 15 is also provided with a temperature insulation cover. The inlet port of the pump three 15 is located at the bottom end of the clamping layer 9, and the outlet end is inserted into the cold storage tank 1.

[0063] In the embodiment, the pressure relief valve is an electric valve, and a gas pressure sensor is arranged in the sealing chamber 3 and electrically connected with the electric valve. When the gas pressure in the sealing chamber 3 reaches a set value, the electric valve is opened to release pressure.

[0064] According to the above technical scheme:

[0065] In use, the electromagnetic reversing valve 20 is simultaneously connected with the pipe two 12 and the pipe three 13, so that liquid nitrogen is circulated into the shunt pipe 19 on one side to exchange heat with the cold storage pipe through the clamping plate 6, and the liquid nitrogen in the pipe three 13 is atomized by the atomizing nozzle 18 and then enters the sealing chamber 3 on the other side, so that the gaseous nitrogen contacts the cold storage pipe, the contact range of the cold storage pipe with the low-temperature nitrogen is expanded, and then the cooling efficiency is improved. By adjusting the atomization efficiency, the cooling efficiency can be controlled, and the control range is wider. The content of the gaseous nitrogen in the sealing chamber 3 gradually increases, in order to avoid the safety hazard caused by continuous pressure increase, when the gas pressure in the sealing chamber 3 reaches a certain degree, the pressure relief valve is automatically opened to guide the gaseous nitrogen into the interlayer 9, so as to achieve the purpose of pressure relief. The gaseous nitrogen accumulates in the interlayer 9, is cooled and liquefied. The newly liquefied liquid nitrogen is pumped back into the cold storage tank 1 by the pump three 15, and is continuously used, so that resources are saved and waste is avoided.

[0066] In the further embodiment of the scheme, a plurality of elastic supporting blocks 8 are uniformly distributed on the bottom wall of the sealing chamber 3. The bottom end of the cold storage pipe is supported by the elastic supporting blocks 8, so as to avoid the cold storage pipe from being knocked and damaged due to shaking and touching the wall, and to ensure that the bottom end of the cold storage pipe is fully contacted by the gaseous nitrogen to ensure uniform cooling efficiency.

[0067] In the further embodiment of the scheme, a plurality of vertical gaps are vertically and spacedly arranged on the side of the clamping plate 6 facing the center of the sealing chamber 3. The gaseous nitrogen can contact the cold storage pipe through the gaps, the contact area between the gaseous nitrogen and the cold storage pipe is increased, and then the uniform cooling of the cold storage pipe is ensured as much as possible.

[0068] In order to avoid that the content of the gaseous nitrogen injected into the interlayer 9 is too high to cause high gas pressure, which easily leads to the bursting of the tank wall of the cold storage tank 1. In the embodiment, a plurality of heat-conducting plates 21 are embedded in the inner wall of the cold storage tank 1 and inserted into the interlayer 9. The heat-conducting plates 21 are made of cold-conducting materials, such as copper 401, aluminum 237, iron 80 and tin 67. One end of the heat-conducting plate 21 is inserted into the cold storage tank 1, and the other end extends into the interlayer 9. The heat-conducting plate 21 contacts and exchanges heat between the liquid nitrogen in the cold storage tank 1 and the gaseous nitrogen in the interlayer 9, so as to accelerate the gathering and condensation of the gaseous nitrogen into liquid nitrogen.

[0069] In Example 6, a further embodiment of this solution, to prevent the inlet of the cold storage tube from being submerged in liquid nitrogen, which could cause a rapid temperature rise and rupture during revival, a horn cover 17 is provided at the bottom of the cap 5. The diameter of the horn cover 17 is larger than the diameter of the space formed by the interlocking of multiple clamps 6, to ensure that the cold storage tube has sufficient placement space and to avoid interference between the horn cover 17 and the upper part of the cold storage tube.

[0070] After the cap 5 is attached, the horn cover 17 is attached to the upper part of the cold storage tube. Liquid nitrogen is injected into the sealed chamber 3 through pipe 11 until the surface of the liquid nitrogen covers the bottom of the horn cover 17. At this point, the gas is concentrated and controlled inside the horn cover 17, while the upper part of the cold storage tube is inside the horn cover 17. The gas in the sealed chamber 3 is compressed and accumulates at the top. Under the action of air pressure, the liquid nitrogen can never rise to the top of the sealed chamber 3, thus exposing the upper end of the cold storage tube to the liquid nitrogen. This avoids the problem of the tube opening being submerged in liquid nitrogen, which could cause the tube to burst due to a rapid temperature rise of a large amount of liquid nitrogen during revival. (When the gas pipe 16 is present, the bottom of the horn cover 17 is located below the gas pipe 16 to reduce the amount of gas overflowing from the gas pipe 16 when the air pressure in the sealed chamber 3 increases. This ensures that there is sufficient air pressure inside the horn cover 17 to suppress the liquid nitrogen level and leave space for the cold storage tube port.)

[0071] Preferably, the upper end of the horn cover 17 is tapered and has a cylindrical hole shape. This tapered part is fastened to the upper port of the cold storage tube, thereby isolating the upper port of the cold storage tube from the inside of the sealing chamber 3 and increasing the tube port isolation characteristics of the cold storage tube.

[0072] Example 7, a further embodiment of this solution, provides a specific structure that enables the clamping plate 6 to achieve shock absorption: several elastic blocks 7 are evenly distributed between the clamping plate 6 and the inner wall of the sealing chamber 3. The elastic blocks 7 are made of low-temperature resistant materials, such as ultra-low temperature (77K) elastic organic crystal materials. By using the elastic blocks 7 to abut against the clamping plate 6, the clamping plate 6 can both clamp the cold storage tube and maintain a certain range of elasticity to achieve the purpose of shock absorption.

[0073] Example 8, a further embodiment of this solution, refers to the appendix. Figure 4 As shown, provided that sufficient heat insulation conditions are set, the can lid 4 has notches that correspond one-to-one with the sealing cap 5. Thus, when the sealing chamber 3 needs to be opened, it is not necessary to open the can lid 4; only the sealing cap 5 needs to be opened, making the entire process more convenient and efficient.

[0074] In Example 9, a further embodiment of this solution, a packing tube 22 extending into the bottom of the cold storage tank 1 is embedded in the axial center of the tank cover 4. The port of the packing tube 22 is provided with a heat-insulating sealing plug. The sealing plug completely covers the upper end of the packing tube 22 to prevent cold conduction from affecting the temperature balance inside the cold storage tank 1.

[0075] In use, the filler pipe 22 can be filled with liquid nitrogen or extracted, for the replenishment of liquid nitrogen and the external disinfection and sterilization treatment of liquid nitrogen, so that the use of the device is more convenient and practical.

[0076] The whole operation principle of the device in embodiments 1-9 is as follows:

[0077] After the cold storage tube is placed in the sealed chamber 3, the pump one 10 corresponding to the sealed chamber 3 is started, and the pump one 10 first draws liquid nitrogen into the pipe two 12 and the pipe three 13:

[0078] The liquid nitrogen in the pipe two 12 flows into the shunt pipe 19, and then flows into the cold storage tank 1 from the end of the shunt pipe 19. In this process, the low temperature of the liquid nitrogen is transferred to the clamping plate 6, and the clamping plate 6 with good cold conducting performance further transfers the low temperature to the cold storage tube, achieving the purpose of slow cooling.

[0079] The liquid nitrogen in the pipe three 13 is atomized from the atomizing nozzle 18 and enters the sealed chamber 3, and the gaseous nitrogen contacts the cold storage tube, expands the contact range of the cold storage tube with the low-temperature nitrogen, and then improves the cooling efficiency. Adjusting the atomization efficiency can control the slow cooling efficiency, which is suitable for tumor tissue cells with different structures, sizes and properties.

[0080] The content of gaseous nitrogen in the sealed chamber 3 gradually increases, in order to avoid the safety hazard of continuous pressure increase, when the air pressure in the sealed chamber 3 reaches a certain degree, the pressure relief valve is automatically opened, and the gaseous nitrogen is guided into the interlayer 9, achieving the purpose of pressure relief. The gaseous nitrogen accumulates in the interlayer 9, liquefies under the cold, and the re-liquefied liquid nitrogen is drawn back into the cold storage tank 1 by the pump three 15 for continuous use.

[0081] When the temperature sensor in the sealed chamber 3 detects that the cooling tube is cooled to the set temperature, the electromagnetic reversing valve 20 is reversed, at this time the pump one 10 injects liquid nitrogen into the pipe one 11 and enters the sealed chamber 3, and the liquid nitrogen accumulates in the sealed chamber 3 to cover the cold storage tube. Under the action of the horn cover 17, the gas in the sealed chamber 3 is extruded and gathered at the top, under the action of air pressure, the liquid nitrogen cannot rise to the top of the sealed chamber 3, so that the upper port of the cold storage tube is exposed to the liquid nitrogen, thereby avoiding the problem that the port of the cold storage tube is immersed in the liquid nitrogen, causing a large amount of liquid nitrogen in the tube to rapidly warm up and cause burst when resuscitating. The cold storage tube is immersed in liquid nitrogen, which will quickly cool to a temperature close to or the same as the temperature of the liquid nitrogen, thereby achieving the purpose of rapid cooling.

[0082] After the cold storage tube is immersed in liquid nitrogen, the pump two 14 is started and maintains the same conveying efficiency as the pump one 10, so that the amount of liquid nitrogen injected into the sealed chamber 3 by the pump one 10 and the amount of liquid nitrogen extracted by the pump two 14 remain the same, so that the liquid nitrogen circulates continuously to ensure good cold conduction effect.

[0083] Embodiment 10, a sealing method using the tumor specimen sealing device, comprising the following steps:

[0084] S1: pre-cooling;

[0085] S2: specimen is put into the sealed;

[0086] S3: slow cooling to a certain temperature;

[0087] S4: rapid cooling to liquid nitrogen temperature.

[0088] The principle details of the embodiment:

[0089] S1: pre-cooling of the cold storage tube to achieve the required cooling speed of vitrification; the support bearing the tumor tissue system is immersed in liquid nitrogen for more than five minutes to make the tissue cool down sufficiently and uniformly in liquid nitrogen.

[0090] S2: specimen processing (fixing, burying, slicing), and then the specimen is put into the pre-cooled cold storage tube, and then the cold storage tube is placed in the sealed chamber 3 and the cover 5 of the sealed chamber 3 is tightly covered.

[0091] S3: pump one 10 starts to pump liquid nitrogen into tube two 12, the liquid nitrogen in tube two 12 flows along the shunt tube 19, and contacts and cools the cold storage tube through the clamping plate 6; or liquid nitrogen enters tube two 12 and tube three 13 at the same time, the liquid nitrogen in tube two 12 flows along the shunt tube 19, and contacts and cools the cold storage tube through the clamping plate 6, and the liquid nitrogen in tube three 13 is atomized by the atomizing nozzle 18, so that the cold storage tube is cooled more evenly. Through the above-mentioned way, the cold storage tube is slowly cooled until a certain temperature.

[0092] S4: the electromagnetic reversing valve 20 makes pump one 10 inject liquid nitrogen into tube one 11, and the liquid nitrogen enters the sealed chamber 3 along tube one 11 to immerse the cold storage tube, so as to achieve rapid cooling to liquid nitrogen temperature.

[0093] In this scheme, when placing the cooling tube, the cover 5 of the sealed chamber 3 needs to be opened, at which time the sealed chamber 3 is filled with air. After the cold storage tube is placed in the sealed chamber 3, the air pressure in the sealed chamber 3 is atmospheric pressure. During the slow cooling stage, the temperature in the sealed chamber 3 is still higher than the liquid nitrogen temperature, at which time the liquid nitrogen gasifies and diffuses in the sealed chamber 3. The gasified liquid nitrogen not only pollutes the liquid nitrogen, but also adheres to the vicinity of the tube opening of the cold storage tube, which is attached to the tube opening of the cold storage tube during the subsequent rapid cooling, which is easy to cause the tube to burst due to the rapid heating of a large amount of liquid nitrogen in the tube during the recovery stage.

[0094] Therefore, in this scheme, a sealed chamber 3 internal air pressure balancing adjusting device is also provided, which comprises:

[0095] A pressure regulating channel 30 is arranged at the center of the cover 5. The pressure regulating channel 30 is provided with a tapered end, and a sealing ball 31 is arranged at the tapered end. A nitrogen spring 32 is arranged below the sealing ball 31 in the pressure regulating channel 30, and the extending end of the nitrogen spring 32 abuts against the bottom end of the sealing ball 31.

[0096] A mounting plate is arranged at the opening of the cover 4, and a cylinder 25 is fixed on the mounting plate. The cylinder 25 is inverted on the mounting plate, and the extending end of the cylinder 25 penetrates through the mounting plate. A sleeve ring is arranged at the extending end of the cylinder 25, and a pressure regulating pipe 24 is fixed in the sleeve ring. The pressure regulating pipe 24 is connected with a pump IV, and the port of the pump IV is connected with a gas tank through a hose. The gas tank stores sterile gas, such as nitrogen, argon and other suitable gas for preserving articles. A top rod 29 is arranged at the lower port of the pressure regulating pipe 24.

[0097] A vacuum pump 33 is arranged on the cover 5, and the suction port of the vacuum pump 33 penetrates through the cover 5, and the exhaust port is connected with the outside.

[0098] After the cover 5 is fastened and sealed, the vacuum pump 33 is started to exhaust the air in the sealing chamber 3. Then the cylinder 25 is started, and the extending end of the cylinder 25 drives the pressure regulating pipe 24 to descend and insert into the pressure regulating channel 30. A sealing ring is arranged between the contact part between the pressure regulating pipe 24 and the inner wall of the pressure regulating channel 30 to avoid leakage of the pressure regulating channel 30. After the pressure regulating pipe 24 is inserted into the pressure regulating channel, the top rod 29 lowers the sealing ball 31 to make the lower port of the pressure regulating channel 30 penetrate through the pressure regulating pipe 24. Then the pump IV is started to inject the gas in the gas tank into the sealing chamber 3 to adjust the pressure in the sealing chamber 3 and exceed the atmospheric pressure. In this way, the contaminated gas in the sealing chamber 3 is exhausted, and the sterile gas is injected. Thus, the mixing of liquid nitrogen and air is avoided to affect the sterile storage condition, and the pressure in the sealing chamber 3 is increased. Under normal pressure, the liquid nitrogen will gasify when the temperature is lower than the temperature requirement, and when the pressure is increased, the temperature requirement for gasification is also increased to ensure that the liquid nitrogen entering the sealing chamber 3 maintains the liquid flow state and is not easily gasified. After the pressure regulation is completed, the cylinder 25 is retracted to drive the pressure regulating pipe 24 to ascend, and the sealing ball 31 ascends to abut against the tapered end under the action of the nitrogen spring 32 to seal the pressure regulating channel 3.

[0099] Preferably, the lower end of the collar abuts a downward spring 26, and the bottom end of the downward spring 26 is provided with a pressing claw 27. The pressing claw 27 is in the shape of a four-claw inverted buckle. A buckle claw 28 is provided around the upper end of the cover 5 at the pressure regulating channel 30, and the buckle claw 28 is provided with a plurality of legs abutting the surface of the cover 5. When the extended end of the cylinder 25 is lowered, the pressing claw 27 first contacts the buckle claw 28, and when the pressure regulating tube 24 is inserted into the pressure regulating channel 30, the pressing claw 27 is trapped on the cover 5 by the buckle claw 28, and the downward spring 26 is compressed, thereby tightly fastening the cover 5. After the pressure regulation is completed, the extended end of the cylinder 25 is retracted to separate the jacking rod 29 from the sealing ball 31, at which time the downward spring 26 is still in the compressed state and still tightly fastens the cover 5 by the pressing claw 27 and the buckle claw 28. The cylinder 25 is electrically connected with the gas pressure sensor in the sealing chamber 3 and the general controller. In this way, the cover 5 is blocked by the cylinder 25 to ensure that when the cold storage tube is to be taken out, the cover 5 can only be opened after the liquid nitrogen in the sealing chamber 3 is completely removed, so that the pressing claw 27 and the buckle claw 28 are separated, at which time the fastening can be unlocked to open the cover 5, thereby avoiding the opening of the cover 5 before the liquid nitrogen is completely removed, which leads to the gasification and overflow of the liquid nitrogen and waste.

[0100] Preferably, the pressure regulating channel 30, the sealing ball 31 and the pressure regulating tube 24 are all covered with a heat insulation layer to prevent the leakage of cold gas.

[0101] In the present scheme, an electromagnetic valve is provided between all the pumps and the corresponding pipelines to ensure that the pipelines are in a closed state when the pumps are closed.

[0102] The above description is only a preferred specific implementation of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can make equivalent replacements or changes to the technical scheme and the application concept of the present application within the technical scope disclosed by the present application, which should be covered by the protection scope of the present application.

[0103] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0104] Although the embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary, and are not to be interpreted as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. A tumor specimen sealing device, characterized by, The cold storage tank (1) and the tank cover (4) are provided with a heat insulation layer, the cold storage tank (1) is provided with a heat insulation sealing plate (2) at the port, the sealing plate (2) is embedded with a plurality of sealed chambers (3), a plurality of shockproof clamping plates (6) are arranged in the sealed chambers (3) in a ring array, the sealed chambers (3) are provided with a cover (5) at the port, a pump one (10) corresponding to the sealed chambers (3) is arranged on the inner bottom wall of the cold storage tank (1), the outlet end of the pump one (10) is communicated with an electromagnetic reversing valve (20), the outlet of the electromagnetic reversing valve (20) is respectively communicated with a pipe one (11) and a pipe two (12), the upper end of the pipe one (11) is inserted into the sealed chamber (3), the bottom end of the sealed chamber (3) is communicated with a pump two (14), the upper end of the pipe two (12) is communicated with a shunt pipe (19) corresponding to the clamping plate (6), the other end of the shunt pipe (19) is wrapped around the corresponding clamping plate (6) and then penetrates through the bottom wall of the sealed chamber (3) and extends out. The sealed chamber (3) is also provided with an internal pressure balance adjusting device, which comprises a pressure regulating channel (30) arranged on the axis of the cover (5), a tapered recess is arranged in the pressure regulating channel (30), a sealing ball (30) is arranged at the tapered recess, a nitrogen spring (32) is arranged below the sealing ball (31) in the pressure regulating channel (30), and the extended end of the nitrogen spring (32) abuts against the bottom end of the sealing ball (31); the installation plate is arranged at the opening of the tank cover (4), the air cylinder (25) is fixed on the installation plate, the air cylinder (25) is inverted on the installation plate and the extended end of the air cylinder (25) penetrates through the installation plate, the extended end of the air cylinder (25) is provided with a sleeve ring, the sleeve ring is fixed with a pressure regulating pipe (24), the pressure regulating pipe (24) is connected with a pump four, the port of the pump four is connected with a gas tank through a hose, the gas tank stores sterile gas, and the bottom port of the pressure regulating pipe (24) is provided with a top rod (29).

2. The tumor specimen sealing device of claim 1, wherein, The cold storage tank (1) is provided with a sandwich layer (9), the lower end of the sandwich layer (9) is communicated with a pump three (15) of the cold storage tank (1), the upper end of the sandwich layer (9) is communicated with a gas pipe (16) of the sealed chamber (3), the gas pipe (16) is provided with a pressure relief valve, the outlet of the electromagnetic reversing valve (20) is also communicated with a pipe three (13), the pipe three (13) is inserted into the sealed chamber (3) and is provided with an atomizing nozzle (18) at the port.

3. The tumor specimen sealing device of claim 2, wherein, The inner bottom wall of the sealed chamber (3) is uniformly provided with a plurality of elastic supporting blocks (8).

4. The tumor specimen sealing device of claim 2, wherein, The clamping plate (6) is vertically and spacedly provided with a plurality of openings on the side facing the center of the sealed chamber (3).

5. The tumor specimen sealing device of claim 2, wherein, The inner wall of the cold storage tank (1) is embedded with a plurality of heat-conducting plates (21) inserted into the sandwich layer (9).

6. The tumor specimen sealing device of claim 1, wherein, The bottom end of the cover (5) is provided with a horn cover (17).

7. The tumor specimen sealing device of claim 1, wherein, A plurality of elastic blocks (7) are uniformly arranged between the clamping plate (6) and the inner wall of the sealed chamber (3).

8. The tumor specimen sealing device of claim 1, wherein, The tank cover (4) is provided with an opening corresponding to the cover (5).

9. The tumor specimen sealing device of claim 1, wherein, The axis of the tank cover (4) is embedded with a filler pipe (22) extending into the bottom end of the cold storage tank (1).

10. A sealing method using the tumor specimen sealing device according to any one of claims 1-9, comprising the following steps: S1: precooling; S2: specimen is put into the sealing; S3: slow cooling to a certain temperature; S4: rapid cooling to liquid nitrogen temperature.

Images