Cuttage breeding method for rhododendron mucronatum

Abstract

The invention discloses a cuttage breeding method for rhododendron mucronatum, and belongs to the technical field of seedling breeding. According to the method, the influence of different rooting powder, cutting length, soaking time and cutting age on the rooting propagation of the cutting of the rhododendron mucronatum is compared and analyzed, reference can be provided for wild domestication of the rhododendron mucronatum, and finally, the rooting powder is determined to be double-Gel No. 6 with the concentration being 100 mg / L, the length of the cutting is 5-8 cm, and the soaking time is 1.5-2 h; the age of the cutting slips is current year.

Description

Technical Field

[0001] The invention relates to the technical field of seedling propagation, and in particular to a cutting propagation method for Rhododendron pubescens. Background Art

[0002] Rhododendron championae Hook. is an evergreen shrub, 2-5 meters high; branches are brown, covered with spreading glandular bristles and short soft hairs, leaves are thick and papery, oblong-lanceolate, edges are densely covered with long bristles and sparse glandular hairs, corolla is white or light red, narrow funnel-shaped, grows in sparse forests in valleys at an altitude of 500-1300 meters, prefers a cool and humid climate, and hates extreme heat and dryness. Rhododendron championae Hook. has many and beautiful flowers and can be cultivated for garden viewing.

[0003] In order to further improve the garden and ecological applications of Rhododendron spinulosum, the introduction of Rhododendron spinulosum down the mountain has become a hot area. The development of Rhododendron in China mainly focuses on the development and utilization of common Rhododendron garden varieties, as well as the ecological habits, cold resistance, drought resistance and other physiological indicators of Rhododendron spinulosum. However, these studies have not been effective in the propagation of Rhododendron spinulosum. This may be related to the secretions of Rhododendron spinulosum itself. In high mountain areas, its roots may secrete different growth hormones or inhibitors. After going down the mountain, this secretion decreases or other conditions inhibit the growth of the root system of Rhododendron spinulosum, which is a great obstacle to the propagation and promotion of Rhododendron spinulosum. Rhododendron spinulosum has different biological and ecological characteristics from other varieties of Rhododendron. It is a high-altitude Rhododendron that is difficult to root. There are no reports on its cutting propagation and seedling promotion technology at home and abroad. Summary of the invention

[0004] The main purpose of the invention is to propose a cutting propagation method for Rhododendron pilosa, compare and analyze the effects of different rooting powders, cutting lengths, soaking times and cutting ages on the rooting and propagation of Rhododendron pilosa cuttings, and provide a reference for the cutting propagation of Rhododendron pilosa.

[0005] To achieve the above-mentioned purpose, the present invention proposes a method for propagating Rhododendron pilosa by cuttings, comprising the following steps: cutting Rhododendron pilosa cuttings, soaking the cuttings in rooting powder solution and then inserting them into a matrix for rooting culture; the length of the cuttings is 3-10 cm; the rooting powder solution is one of the aqueous solution No. 6 and ABT-1 aqueous solution with a concentration of 50-150 mg / L; the soaking time is 10s-2h.

[0006] Preferably, the cutting propagation method of Rhododendron spinulosa comprises the following steps:

[0007] S1. Mix river sand, perlite and loess as cutting medium, and add carbendazim powder into the medium to make a seedbed. When arranging the seedbed, the middle is slightly higher and slightly tilted to both sides, with cement enclosures on both sides;

[0008] S2, cutting Rhododendron spinulosum cuttings; soaking the cuttings in rooting powder and inserting them into the seedbed;

[0009] S3. After cuttings, make an arch shed with bamboo strips along the cement enclosure on the cutting bed, then cover the arch shed with a thin transparent mulch, cover both sides of the mulch with loess, and after covering it tightly, observe the water vapor state on the mulch. When the water vapor on the mulch decreases, lift the mulch and water it. When encountering continuous rainy weather, prevent diseases and choose a sunny day to spray carbendazim for disinfection. Remove moldy cuttings in time; after raising seedlings and hardening them, they can be transplanted and planted.

[0010] Preferably, the matrix is ​​a mixture of river sand, perlite and loess in a mass ratio of 4-6:1-3:2-4.

[0011] Preferably, carbendazim powder is added to the matrix for disinfection.

[0012] Preferably, the amount of carbendazim powder added is 5-10% of the mass of the substrate.

[0013] Preferably, the amount of bacterium chloride powder added is 8% of the mass of the matrix.

[0014] Preferably, the age of the cuttings is one of one-year-old, two-year-old and three-year-old.

[0015] Preferably, the age of the cuttings is one year old.

[0016] Preferably, after the cutting in step S3, the temperature in the greenhouse is controlled to be 20-25°C, the humidity is 70-75%, and the pH value is 5.0-5.4.

[0017] Preferably, after the cutting in step S3, the temperature in the greenhouse is controlled to be 25° C., the humidity is 75%, and the pH value is 5.2.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The present invention establishes a set of efficient and rapid propagation methods for high-quality seedlings of Rhododendron spinulosum from the aspects of substrate selection, cutting selection and treatment, water and fertilizer, and pest and disease management, which is of great significance for the large-scale production of Rhododendron spinulosum;

[0020] (2) The present invention compares and analyzes the effects of different rooting powders, cutting lengths, soaking times and cutting ages on the rooting reproduction of Rhododendron pilosa cuttings, which can provide a reference for the wild domestication of Rhododendron pilosa; the rooting powder finally determined is Shuangjier No. 6 with a concentration of 100 mg / L, the cutting length is 5-8 cm, the soaking time is 1.5h-2h; the age of the cuttings is the current year. DETAILED DESCRIPTION

[0021] To avoid redundancy, the items used in the following examples are all commercially available products unless otherwise specified, and the methods used are all conventional methods unless otherwise specified.

[0022] The cuttings of Rhododendron pubescens were obtained from Hunan Forest Botanical Garden, and the planting coordinates were 113°01′30″E, 28°06′40″N.

[0023] Example 1

[0024] A method for propagating Rhododendron pubescens by cuttings comprises the following steps:

[0025] S1. Mix river sand, perlite and loess in a mass ratio of 5:2:3 as a cutting medium, and add carbendazim powder into the medium to make a seedbed. The amount of carbendazim powder added is 8% of the mass of the medium. When arranging the seedbed, the middle is slightly higher and slightly tilted to both sides for drainage. The seedbed is 12m×2m, with 20cm high cement enclosures on both sides;

[0026] S2, collect 50 one-year-old cuttings, cut into 5-8 cm cuttings, retain two buds, soak the cuttings in 100 mg / L Shuangjier No. 6 aqueous solution, soak for 1.5 hours and then dry naturally in the shade; then insert the cuttings into the seedbed;

[0027] S3. After cuttings, make an arch shed with bamboo strips along the cement enclosure on the cutting bed, and then cover the arch shed with a thin transparent mulch, cover both sides of the mulch with loess, and after covering it tightly, pay attention to the water vapor state on the mulch, control the temperature in the shed to 25℃, humidity to 75%, and pH value to 5.2. When the water vapor on the mulch decreases, pay attention to lift the mulch and water it. When encountering continuous rainy weather, pay attention to prevent diseases, choose a sunny day to spray carbendazim for disinfection, and remove moldy cuttings in time; after raising seedlings, they are hardened and then transplanted and planted. Water them thoroughly once, and then water them once a week. The survival rate was counted after 30 days, and the survival rate was 98.6%.

[0028] Test Example 1

[0029] Observe the effect of different rooting solution treatments on the rooting of Rhododendron pilosa cuttings:

[0030] 60 one-year-old cuttings were collected from Hunan Forest Botanical Garden, cut into 5-8 cm cuttings, retaining two buds, and divided into 3 groups, namely, a water treatment group, a 100 mg / L Shuangjier No. 6 group, and a 100 mg / L ABT-1 group. The cuttings were soaked in 100 mg / L Shuangjier No. 6 aqueous solution, an ABT-1 aqueous solution, and clean water, respectively, and dried naturally in the shade after soaking for 2 hours; then the cuttings were inserted into the seedbed; the cuttings were managed according to the method in Example 1, and the rooting rate and rooting quality of each treatment were counted. The results are shown in Table 1:

[0031] Table 1 Experimental results on the effects of different rooting solutions on the rooting of Rhododendron pilosa cuttings

[0032] Rooting powder Rooting rate (%) Average number of roots Longest adventitious root (cm) Double Gill No. 6 81.23Aa 13.21Aa 2.61Aa ABT-1 72.31Bb 9.68Bb 1.54Aa Shimizu 30.46Bb 4.32Bb 0.92Aa

[0033] From Table 1, it can be seen that the best rooting rate is in the GGR 6 treatment group, with a rooting rate of 81.23% and an average number of roots of 13.21. There is no significant difference in the rooting rate and average adventitious roots obtained by the cuttings treated with GGR 6 and ABT1 (P>0.05). However, in overall comparison, the rooting rate and average number of adventitious roots of GGR 6 are better than those of ABT-1, and both are better than the clear water control group.

[0034] Test Example 2

[0035] Observe the effect of different rooting solution soaking time on the rooting of Rhododendron pilosa cuttings:

[0036] 50 one-year-old cuttings were collected from Hunan Forest Botanical Garden, cut into 5-8 cm cuttings, retaining two buds, and immersed in 100 mg / L Shuangjier No. 6 aqueous solution. The cuttings were divided into 5 groups, and the experimental group was divided into 5 groups. The immersion time was 10s, 0.5s, 1h, 1.5h, and 2h respectively; then the cuttings were inserted into the seedbed; the cuttings were managed according to the method in Example 1, and the rooting rate and rooting quality of each treatment were counted. The results are shown in Table 2:

[0037] Table 2 Study on the effect of different soaking time on rooting of cuttings

[0038]

[0039]

[0040] It can be seen from the experimental data in Table 2 that the rooting rate increases with the extension of the soaking time. The rooting rate of the cuttings treated with immersion for 1.5 hours is the highest, and the lowest is the cuttings soaked for 10 seconds. The rooting rate of the two treatments differs by 33.28%. There is no significant difference in the rooting rate, average adventitious roots and longest adventitious roots between 1.5 hours and 2 hours (P>0.05). The results show that within a certain concentration range, the longer the soaking time, the longer the hormone will prolong the rooting rate of the cuttings. The effect of soaking GGR6 for 2 hours is relatively better, which is manifested in the large number of roots, longer root length and high rooting rate.

[0041] Test Example 3

[0042] Observation of the effects of Rhododendron spinulosum on rooting of cuttings in different years:

[0043] 20 one-year-old, 20 two-year-old, and 20 three-year-old cuttings were collected from Hunan Forest Botanical Garden, cut into 5-8 cm cuttings, retaining two buds, and rooting treatment and cutting management were performed according to the method in Example 1. The rooting rate and rooting quality of each treatment were statistically analyzed. The results are shown in Table 3:

[0044] Table 3 Study on the influence of Rhododendron spinulosum on rooting of cuttings in different years

[0045] Cutting age Rooting rate (%) Average number of roots Longest adventitious root (cm) First Year 86.23Aa 13.26Aa 11.26Aa Two-year student 52.34Aa 10.98Aa 9.73Bb Third year student 48.65Aa 8.23Aa 8.43Bb

[0046] From the experimental data in Table 3, it can be seen that the rooting rate of one-year-old Rhododendron pilosa cuttings is the highest, far greater than that of two-year-old and three-year-old cuttings; however, the survival rate of two-year-old and three-year-old cuttings is not much different, both of which are about 50%, and the root length and number of rooted branches of the two are also far less than that of one-year-old cuttings. This may be because the rooting effect of its lignified branches is significantly better than that of other slightly older years, and it maintains a certain rooting activity.

[0047] Test Example 4

[0048] Observation of the effect of different cutting lengths on rooting:

[0049] 60 one-year-old Rhododendron spinulosum were collected from Hunan Forest Botanical Garden. The experiment was divided into 3 groups, one group was cut into 3-5 cm (one bud node), one group was cut into 6-8 cm (two bud nodes), and one group was cut into 8-10 cm (three bud nodes). The cuttings were soaked in GGR No. 6 for 1.5 h and the cuttings were cut and managed according to the method in Example 1. The rooting rate and rooting quality of each treatment were counted, and the statistical results were averaged. The results are shown in Table 4:

[0050] Table 4 Multiple comparisons of rooting characteristics of cuttings of different lengths

[0051]

[0052] From the experimental data in Table 4, it can be seen that the rooting rate of cuttings with a length of 3-5 cm is significantly lower, but the survival rate of cuttings above 5 cm is similar, among which the rooting rate of 5-8 cm is slightly greater than that of 8-10 cm; the root length and the average number of roots also show similar results. This may be because the cuttings are too long, which accelerates the metabolism of the cuttings and consumes their own nutrients and water, making the cuttings prone to withering and dying; if the cuttings are too long, the nutrients and water absorbed are insufficient; if the cuttings are too short, the nutrients supplied to themselves in the early stage are insufficient, and it is difficult to support the growth of new roots.

[0053] In summary, the present invention compares and analyzes four physiological indices that may affect the cuttings of Rhododendron pilosa, providing a reference for the wild domestication of Rhododendron pilosa. The establishment of the above technical system has important application value for the rapid propagation of Rhododendron pilosa seedlings.

[0054] The above are only preferred embodiments of the present invention, and do not limit the patent scope of the present invention. For those skilled in the art, the present invention may have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the patent protection scope of the present invention.

Claims

1. A method for propagating Rhododendron pilosa by cuttings, characterized in that: The steps include: Cut the cuttings of Rhododendron pubescens, soak them in rooting powder solution and then insert them into a matrix for rooting culture; the length of the cuttings is 3-10 cm; the rooting powder solution is one of Shuangjier 6 aqueous solution and ABT-1 aqueous solution with a concentration of 50-150 mg / L; the soaking time is 10s-2h.

2. The cutting propagation method of Rhododendron pilosa L. according to claim 1, characterized in that: The steps include: S1. Mix river sand, perlite and loess as cutting medium, and add carbendazim powder into the medium to make a seedbed. When arranging the seedbed, the middle is slightly higher and slightly tilted to both sides, with cement enclosures on both sides; S2, cutting Rhododendron spinulosum cuttings; soaking the cuttings in rooting powder and inserting them into the seedbed; S3. After cuttings, make an arch shed with bamboo strips along the cement enclosure on the cutting bed, then cover the arch shed with a thin transparent mulch, cover both sides of the mulch with loess, and after covering it tightly, observe the water vapor state on the mulch. When the water vapor on the mulch decreases, lift the mulch and water it. When encountering continuous rainy weather, prevent diseases and choose a sunny day to spray carbendazim for disinfection. Remove moldy cuttings in time; after raising seedlings, they are hardened and then transplanted out of the nursery.

3. The cutting propagation method of Rhododendron tricholoma according to claim 1, characterized in that: The matrix is ​​a mixture of river sand, perlite and loess in a mass ratio of 4-6:1-3:2-4.

4. The cutting propagation method of Rhododendron tricholoma according to claim 1, characterized in that: Carbendazim powder is added into the matrix for disinfection.

5. The cutting propagation method of Rhododendron tricholoma according to claim 4, characterized in that: The amount of the carbendazim powder added is 5-10% of the mass of the matrix.

6. The cutting propagation method of Rhododendron tricholoma according to claim 4, characterized in that: The amount of the carbendazim powder added is 8% of the mass of the substrate.

7. The cutting propagation method of Rhododendron tricholoma according to claim 1, characterized in that: The age of the cuttings is one of one-year-old, two-year-old and three-year-old.

8. The cutting propagation method of Rhododendron tricholoma according to claim 7, characterized in that: The age of the cuttings is one year old.

9. The cutting propagation method of Rhododendron tricholoma according to claim 2, characterized in that: In step S3, after the cutting, the temperature in the greenhouse is controlled to be 20-25° C., the humidity to be 70-75%, and the pH value to be 5.0-5.

4.

10. The cutting propagation method of Rhododendron spinulosa according to claim 9, characterized in that: In step S3, after the cutting, the temperature in the greenhouse is controlled to be 25° C., the humidity to be 75%, and the pH value to be 5.2.

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