Method for eliminating chickens with abnormal invisible tissues or diseased chickens in early stage

Abstract

The invention provides a method for early elimination of chickens with abnormal invisible tissues or pathological changes, which comprises the following steps: S1, determining detection indexes, laying the chickens on an experiment table with the backs facing downwards, removing feathers at action sites, and uniformly smearing a coupling agent; the detection indexes comprise liver, abdominal cavity, and / or ovary, and / or fallopian tube; s2, setting detection parameters of a Doppler diasonograph, and detecting the chicken by using a probe; and S3, observing in the inspection process, storing and recording a corresponding detection result, and eliminating the chicken with abnormal invisible tissues or pathological changes according to the detection result. The method provided by the invention can diagnose the abnormality or lesion of the liver, abdominal cavity, ovary and fallopian tube of the chicken in different breeding periods, can timely discover the hidden abnormality and no obvious disease symptom and behavior characteristics of the chicken, and screens out and eliminates the abnormal abnormality and no obvious disease symptom and behavior characteristics, thereby effectively reducing the breeding cost, improving the breeding benefit, and improving the economic benefit of the chicken. And unnecessary breeding economic loss is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of chicken breeding, and in particular to a method for early elimination of chickens with invisible tissue abnormalities or lesions. Background Art

[0002] In recent years, with the continuous development of the fast food industry, the demand for broiler chickens has continued to increase. The broiler breeding industry has become an important part of animal husbandry. In order to better manage broiler chickens, they often need to be concentrated and divided into groups for breeding. During the breeding process, due to the influence of environmental factors, the growth rate of chickens is different, and they also carry various diseases. In the process of broiler breeding, The health monitoring of broiler chickens is often done through external observation of activity status through experience, so that only a rough judgment of the health of the chickens can be made, but it is often impossible to make a direct judgment on the abnormalities or lesions in the internal tissues. Or some detection methods are to randomly select a fixed number of broiler chickens to check various physiological indicators, obtain the health status of the selected chickens, and thus roughly judge the health status of the entire flock, which cannot comprehensively detect the health of the flock, and often cannot screen out chickens with early tissue abnormalities or lesions, resulting in inconsistent growth status of the flock, which invisibly increases the cost of raising chickens. Therefore, there is an urgent need for a method for early elimination of chickens with invisible tissue abnormalities or lesions, which can screen out chickens with invisible tissue abnormalities or lesions at an early stage and eliminate them, thereby effectively avoiding the death of chickens and improving the economic efficiency of breeding. Summary of the invention

[0003] In view of this, the present invention proposes a method for early elimination of chickens with invisible tissue abnormalities or lesions. The method of the present invention can effectively screen out chickens with invisible tissue abnormalities or lesions at an early stage and eliminate them, thereby reducing breeding costs and improving breeding benefits.

[0004] The technical solution of the present invention is achieved in this way: A method for early elimination of chickens with invisible tissue abnormalities or lesions, comprising the following steps: S1. Determine the detection index, lay the chicken on the experimental table with its back facing downward, remove the feathers at the action site and evenly apply the coupling agent; the detection index includes the liver, abdominal cavity, and / or ovary, and / or oviduct; S2, after setting the detection parameters of the Doppler ultrasonic diagnostic instrument, use the probe to detect the chickens; the detection parameters are set as follows: focus position 3-4, grayscale mapping 1-2, pseudo color 3-4, dynamic range 115-116, scanning range 0-1, scanning density, noise suppression 1-2, frame correlation 2-3, line average 2-3, space composite off, edge enhancement 0-1, image enhancement 0-1; S3. Observe during the inspection process, store and record the corresponding test results, and eliminate chickens with invisible tissue abnormalities or lesions based on the test results.

[0005] Furthermore, the detection parameters are set as follows: focus position 3, grayscale mapping 1, pseudo color 3, dynamic range 115, scanning range 0, scanning density medium, noise suppression 1, frame correlation 2, line averaging 2, spatial composite off, edge enhancement 0, and image enhancement 0.

[0006] Furthermore, when testing the liver, the probe acts on the left and right parts of the skin from the keel ridge to the side edge of the hen; when testing the abdominal cavity, the probe acts on the left, middle and right parts of the skin at the end of the keel process of the hen; when testing the ovary or fallopian tube, the probe acts on the part of the skin from the femoral head on the anatomical right side of the hen to the cloaca.

[0007] Furthermore, the Doppler ultrasound diagnostic instrument is a DCU10 digital Doppler ultrasound diagnostic instrument.

[0008] Furthermore, the probes used by the Doppler ultrasonic diagnostic instrument during the detection process include a phased array probe and a linear array probe; the phased array probe has F=8.0 MHz and D=60 mm; the linear array probe has F=9.0 MHz and D=40 mm.

[0009] Furthermore, during the detection process, the phased array probe is gently placed to use the transverse up and down swing scanning method to observe the left and right lobes of the liver, the linear array probe is gently placed to use the sequential continuous parallel section method to observe the abdominal cavity, and the three-dimensional fan-shaped section method is used to observe the ovary or fallopian tube through the phased array probe with uniform force of skin elasticity. Further, in step S3, the B model is used for observation, and after clear sound and image are clearly observed, they are stored as pictures in TIFF format and as videos in AVI format. The liver, abdominal cavity, and / or ovary, and / or oviduct of the chicken are observed through the video, so as to judge the abnormality or pathological condition of the chicken tissue.

[0010] Furthermore, the criteria for determining liver abnormalities or lesions are as follows: using a Doppler ultrasound diagnostic apparatus to observe that the ultrasound acoustic image characteristics of the left and right lobes of the liver are irregular and scattered high-echo areas, blurred edges of low-echo areas, occasional anechoic lesions, and uneven parenchymal echoes.

[0011] Furthermore, the criteria for judging the abdominal abnormality or lesion are: small or medium-sized abdominal effusion or cecal distension, reticular fiber separation of fluid areas, and jelly-like punctate high echoes.

[0012] Furthermore, the criteria for determining ovarian abnormalities or lesions are: follicles lose high and low echo nested structures, are elliptical in shape, contain large dark areas, and have strong heterogeneous echoes distributed between follicles.

[0013] Furthermore, the fallopian tube abnormality or lesions are judged through the egg formation process, and the judgment criteria are divided into ampulla to isthmus lesions or fimbria development disharmony or ovarian development disharmony; among them, the judgment criteria for ampulla to isthmus lesions are: the yolk is degenerated and the central part of the egg is homogeneous and highly echoic and scattered, and mixed with anechoic areas; the judgment criteria for fimbria development disharmony are: multiple eggs appearing in the eggshell gland at the same time in ultrasound images; the judgment criteria for ovarian development disharmony are: irregular follicle ultrasound images.

[0014] Furthermore, the entire testing process is carried out in a quiet environment, with gentle movements and completed in a short time to avoid stress and injury to the chickens.

[0015] Furthermore, the feathers at the probe action site are removed before testing. The feathers should be removed to remove the feather roots inside the skin without bleeding, and then the coupling agent is evenly applied to expel air and increase the lubrication effect.

[0016] Furthermore, the Doppler ultrasound diagnostic instrument should connect the two probes before turning on, and check various parameters such as gain, depth, frequency, pseudo-color scanning speed, etc. after turning on the instrument to keep them consistent throughout the inspection process.

[0017] Furthermore, when the Doppler ultrasound diagnostic instrument has blurred images during the diagnosis process, the sound wave signal can be enhanced by adding coupling agent, increasing the frequency, or reducing the time gain compensation to reduce the signal-to-noise.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The method of the present invention can diagnose abnormalities or lesions in the liver, abdominal cavity, ovaries and oviducts of chickens at different breeding periods, and can timely discover hidden abnormalities of chickens without obvious symptoms and behavioral characteristics, screen them out and eliminate them, thereby effectively reducing breeding costs, improving breeding benefits, and avoiding unnecessary breeding economic losses.

[0019] The present invention can effectively improve the accuracy of the Doppler ultrasonic diagnostic instrument during the detection process through a scientific and reasonable detection method and specific parameter settings, thereby improving the detection efficiency.

[0020] This method has the advantages of low cost, high speed and low stress, and is suitable for long-term, multiple and tracking aquaculture monitoring and inspection. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The figure is a schematic diagram of the inspection parts, detection probes and detection techniques of hens; Figure A is the liver inspection, Figure B is the ovary inspection, Figure C is the fallopian tube inspection, and Figure D is the abdominal cavity inspection.

[0022] Figure 2The ultrasound images and anatomical diagrams of the hen's liver are shown in Figure A1 and A2, respectively. Figures B1 and C1 are both ultrasound images of abnormal livers, and Figures B2 and C2 are both anatomical diagrams of abnormal livers. The arrow in Figure B1 points to uneven high echoes in the substance, and the arrow in Figure C1 points to scattered pore-like low echoes.

[0023] Figure 3 The following are ultrasound images and anatomical diagrams of the hen's abdominal cavity; Figures A1-D1 are ultrasound images of the abnormal abdominal cavity, and A2-D2 are anatomical diagrams corresponding to the abnormal abdominal cavity. The arrows in Figures A1 and A2 point to lattice-like effusions, the arrows in Figures B1 and B2 point to ascites, the arrows in Figures C1 and C2 point to jelly-like yolk, and the arrows in Figures D1 and D2 point to bloated cecum.

[0024] Figure 4 The ultrasound images and anatomical diagrams of hens' ovaries; Figures A1 and A2 are the ultrasound images and anatomical diagrams of normal ovaries, respectively; Figures B1-J1 and B2-J2 are the ultrasound images and corresponding anatomical diagrams of abnormal ovaries, respectively. The arrows in Figures B1 and B2 point to ovarian hyperplasia, the white asterisks in B1 and B2 point to yolk agglomerations, the arrows in Figures C1 and C2 point to transparent follicles, the arrows in Figures D1 and D2 point to atretic follicles, the arrows in Figures E1 and E2 point to ovarian nodules, the arrows in Figures F1 and F2 point to follicular fluid agglutination, the arrows in Figures G1 and G2 point to two dominant follicles, the arrows in Figures H1 and H2 point to cystic follicles, and the asterisks in Figures J1 and J2 point to degenerated follicles.

[0025] Figure 5 These are ultrasound sonograms and anatomical diagrams of the hen's oviduct; Figure A1 is the sonogram of a normal egg, Figure A2 is the sonogram of an abnormal oviduct, Figures B1-D1 and B2-D2 are the sonograms and corresponding anatomical diagrams of follicles after abnormal ovulation, respectively. The arrow in Figure A2 points to the irregular low-echo area, the arrow in B1 and the blue arrow on the right side of B2 point to the denatured yolk, the red arrow on the left side of B2 points to a normal egg, the arrows in C1 and C2 point to multiple ovulation, and the arrows in D1 and D2 point to intracavitary ovulation.

[0026] Figure 6 is the proportion of abnormal hens in the group; Figures AD are the proportion of abnormal hens in the 19th generation of M-line at 22 weeks of age, 32 weeks of age, 42 weeks of age and 50 weeks of age, respectively, and the control group is healthy individuals examined by ultrasound during the same period.

[0027] Figure 7 Figure A shows the persistence of abnormal conditions in hens; Figure A shows the proportion of abnormalities in the liver, ovaries, oviducts and abdominal cavity of chickens aged 210-222 days; Figure B shows the proportion of hens with multiple abnormalities; Figures C and D show the intersection of abnormal and normal chickens at 22 weeks, 32 weeks and 42 weeks, respectively.

[0028] Figure 8 The effect of the detection parameter settings of Example 1 and Comparative Example 1 when the DCU10 ultrasonic diagnostic instrument performs detection. Figures AD are the sonograms of the liver, ovary, fallopian tube and abdominal cavity observed by the detection parameters provided by Comparative Example 1. Figure 8 E is the accuracy comparison result when using the detection parameters provided in Example 1 and the detection parameters provided in Comparative Example 1. DETAILED DESCRIPTION

[0029] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0030] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0031] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0032] The coupling agent manufacturer of the present invention is: Tianjin Yajin Technology Development Co., Ltd., and the name is TM-100 medical ultrasonic coupling agent.

[0033] The chickens used in the implementation of the present invention are 19th generation hens of the M series provided by the Luoniushan Breeding Farm of Hainan Tanniu Wenchang Chicken Co., Ltd. The feeding and management of the chickens meet the actual production standards. The sample size of the chickens is 3,620. Ultrasonic detection and production data collection were carried out at the 22nd, 32nd, 42nd and 50th weeks of age of the chickens, thereby verifying the feasibility of the method of the present application.

[0034] Example 1 - Method for early elimination of chickens with invisible tissue abnormalities or lesions The steps include: S1. Determine the test indicators (including liver, abdominal cavity, ovary and oviduct), and lay the hens flat on the experimental table with their backs facing down (such as Figure 1 As shown), remove the feathers at the affected area and apply coupling agent evenly; S2. After setting the detection parameters of the DCU10 digital Doppler ultrasonic diagnostic instrument, use the probe to detect the chickens; the detection parameters are set as follows:

[0035] The probes used in the detection process include phased array probes and linear array probes; the phased array probe has F=8.0MHz, D=60mm; the linear array probe has F=9.0MHz, D=40mm; when detecting the liver, the probe acts on the left and right parts of the skin from the keel ridge to the side edge of the hen; when detecting the abdominal cavity, the probe acts on the left, middle and right parts of the skin at the end of the keel process of the hen; when detecting the ovary or fallopian tube, the probe acts on the part of the skin from the femoral head to the cloaca on the right side of the hen's anatomy; and during the detection process, the phased array probe is gently placed to observe the left and right lobes of the liver using the transverse up and down swing scanning method, the linear array probe is gently placed to observe the abdominal cavity using the sequential continuous parallel section method, and the ovary or fallopian tube is observed through the phased array probe using the three-dimensional fan-shaped section method with uniform force applied to the skin elasticity; S3. During the inspection, use the B model to observe. After the clear sound and image are clearly observed, store them in TIFF format as pictures and in AVI format as videos. Observe the liver, abdominal cavity, ovary and fallopian tube of the chicken through the video. According to the various judgment criteria, detect the abnormal tissue or pathological conditions of the chicken, so as to eliminate the chickens with invisible tissue abnormalities or lesions. The judgment criteria are as follows: ① The criteria for judging liver abnormalities or lesions are: the liver is observed to be blood red or khaki using Doppler ultrasound diagnostic equipment, and the ultrasound sound image characteristics of the left and right lobes of the liver are irregular and scattered high-echo areas, blurred edges of low-echo areas, occasional anechoic lesions, and uneven parenchymal echoes; ② The criteria for judging abdominal abnormalities or lesions are: small or medium-sized abdominal effusions or cecal distension, reticular fiber separation fluid areas, and jelly-like high echoes; ③ The criteria for judging ovarian abnormalities or lesions are: follicles lose high and low echo nested structures, the shape is oval, containing large dark areas, and strong heterogeneous echoes are distributed between follicles; ④ Abnormalities or lesions of the fallopian tube are judged through the egg formation process, and the judgment criteria are divided into lesions from the ampulla to the isthmus, inharmonious development of the fimbria, or inharmonious development of the ovary. Among them, the judgment criteria for lesions from the ampulla to the isthmus are: the yolk is degenerated and the central part of the egg is homogeneous and highly echoic and scattered, and mixed with anechoic areas; the judgment criteria for inharmonious development of the fimbria are: ultrasound images of multiple eggs appearing in the eggshell gland at the same time; the judgment criteria for inharmonious development of the ovary are: irregular ultrasound images of follicles.

[0036] After the liver, abdominal cavity, ovary and oviduct of the chickens were tested according to the above steps, the corresponding chickens were dissected and observed to verify the feasibility of the method of the application. The experimental results are as follows: Figure 2-6 shown.

[0037] like Figure 2As shown in the figure, the ultrasonic image characteristics of the left and right lobes of the chicken liver detected by Doppler ultrasound diagnostic instrument are irregular and scattered high echo areas, blurred edges of low echo areas, occasional anechoic lesions and uneven echo of the substance. After dissection, it was observed that the chicken liver was blood red or khaki. The former was often accompanied by pinhole hemorrhages or exudative blood spots, and the latter was often accompanied by hyperplasia, hypertrophy, crisp and greasy texture, and was an abnormal chicken.

[0038] like Figure 3 As shown, Doppler ultrasound diagnostic instrument was used to detect the abdominal cavity of chickens. The reticular fiber-separated fluid areas and jelly-like dot-like high echoes in the abdominal cavity of chickens were screened out and dissected. Medium and small abdominal effusions were found in the abdominal cavity of chickens, which are usually caused by respiratory diseases, and the second most common cause is cecal flatulence.

[0039] like Figure 4 As shown, using Doppler ultrasound diagnostic instrument to detect chicken ovaries, abnormal ovaries include many types, they have typical sound and image characteristics, the most common of which are follicular atresia and multi-level follicular redundancy (the number of follicles is greater than or equal to 8), and the diffuse high echo and dense dominant follicles presented by ultrasound sound image are unique to the abnormality of follicular redundancy; follicular cysts, follicular transparency, and follicular nodules mostly appear in the late egg-laying period. This type of abnormal condition shows the same symptoms as serous ovarian cancer, which is reflected in the sound and image characteristics of follicle loss, high and low echo nested structures, elliptical shape, containing large dark areas, and strong heterogeneous echoes distributed between follicles. Therefore, when using the method of the present invention, it should be noted that ovarian examination requires more standardized operation, relatively long-term diagnosis, and accurate image recognition.

[0040] like Figure 5 As shown in the figure, the oviduct is difficult to detect from the outside due to its structural specificity, but its lesions can be largely judged by the egg formation process. Compared with the ultrasound image of normal eggs, the eggs with yolk degeneration have a homogeneous high echo in the center and scattered echo-free areas, which may be caused by lesions from the ampulla to the isthmus. Multiple ovulation and intracavitary ovulation are mostly caused by the uncoordinated development of the fimbria and ovaries. The former is manifested as multiple eggs appearing in the shell gland at the same time, and the latter is manifested as irregular follicle ultrasound images.

[0041] like Figure 6As shown, the liver, abdominal cavity, ovary and oviduct of hens at different stages (22, 32, 42 and 50 weeks of age) were tested by the method of the present invention and abnormal chickens were screened. It was found that the proportion of abnormal groups in the whole group was about 8.36-13.31% at different egg-laying stages. Among various abnormalities, ovarian abnormalities accounted for the largest proportion, followed by abdominal abnormalities. This shows that in the actual production process, a considerable number of sick hens cannot be eliminated by traditional visual observation, palpation and other methods, and these organ development abnormalities almost accompany the entire production cycle of the individual. Although these hidden abnormalities have no obvious symptoms and behavioral characteristics, they can be easily identified by ultrasonic detection. As early as the early stage of egg laying, 8.96% of the hens in the whole group had organ development disorders, which suggests that ultrasonic examinations should be performed early.

[0042] In addition, if Figure 7 As shown in the data, liver abnormalities and fallopian tube abnormalities were relatively persistent and stable, while the proportions of ovarian abnormalities and abdominal abnormalities fluctuated. There were fewer individuals with multiple abnormalities, and the proportion of individuals with two abnormalities increased with age, most of which were ovarian abnormalities. There were 20 individuals with abnormalities in ultrasound examinations at 22 weeks, 32 weeks, and 42 weeks (mostly ovarian and fallopian tube abnormalities), accounting for 2.45% of the abnormal group in all three examinations. Similarly, there were 1,216 healthy individuals, accounting for 32.07%. The results suggest the necessity and early warning of ultrasound examinations, which can be performed as early as the stage of sexual maturity to eliminate potential hazards in advance and avoid unnecessary economic losses.

[0043] Moreover, this method uses Doppler ultrasound diagnostic equipment to detect Wenchang chickens in the early, peak, late and final stages of egg production (i.e., 22 weeks, 32 weeks, 42 weeks and 50 weeks). The results show that diseased individuals with poor production performance can be detected by ultrasound at each stage.

[0044] Finally, in the actual breeding process, attention should be paid to the time density of ultrasound examinations. If the interval is too long, it is easy to ignore new or recurrent organ abnormalities, and if the interval is too short, it is easy to cause cost waste and stress. During the inspection process, it was found that the proportion of diseased groups in the ultrasound diagnosis at an interval of 4 days was stable, so the frequency of ultrasound diagnosis can be appropriately reduced. However, in the ultrasound diagnosis at an interval of 10 weeks, there were fluctuations in the continuously healthy groups. Individuals diagnosed as healthy by ultrasound in the early egg-laying period may become ill again during the peak egg-laying period. Therefore, the interval between ultrasound diagnosis is recommended to be no more than 10 weeks.

[0045] Example 2 - Method for early elimination of chickens with invisible tissue abnormalities or lesions The detection parameters of the ultra-Doppler ultrasonic diagnostic instrument are adjusted on the basis of Example 1, and the detection parameters are set as follows:

[0046] Compared with Example 1, the detection parameter setting of this embodiment can also achieve good detection results. When performing ultrasonic observation, it can be found that the sound and image quality and clarity of the liver, abdominal cavity, ovaries and fallopian tubes are not significantly different from those of the embodiment. When performing ultrasonic diagnosis, the diagnostic accuracy of this embodiment is also not significantly different.

[0047] Comparative Example 1 Compared with Example 1, the detection parameters of the DCU10 digital Doppler ultrasound diagnostic instrument are changed in this comparative example. The frequencies of the phased array probe and the linear array probe in this comparative example are both 6.0 MHz. The detection parameters of this comparative example are set as follows:

[0048] The test results of this comparative example are compared with those of Example 1. Figure 8 shown.

[0049] Depend on Figure 8 It can be seen that when comparative example 1 and embodiment 1 use different parameters for ultrasonic observation, it can be found that the sound and image quality and clarity of the liver, abdominal cavity, ovaries and fallopian tubes are significantly deteriorated. Not only that, when performing ultrasonic diagnosis, the diagnostic accuracy of comparative example 1 is also significantly reduced.

[0050] In summary, the method of the present invention can effectively detect chickens with abnormalities or lesions in the liver, ovary, fallopian tube and abdominal cavity during chicken breeding, and can timely eliminate chickens with poor performance, thereby improving breeding efficiency and promoting the economic benefits of breeding. The method has the advantages of low cost, high speed, low stress, etc., and is suitable for long-term, multiple, tracking breeding monitoring and detection.

[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for early elimination of chickens with invisible tissue abnormalities or lesions, characterized in that: The steps include: S1. Determine the detection index, lay the chicken on the experimental table with its back facing downward, remove the feathers at the action site and evenly apply the coupling agent; the detection index includes the liver, abdominal cavity, and / or ovary, and / or oviduct; S2, after Doppler ultrasonic diagnostic instrument is set to detection parameters, use probe to detect chickens; Described detection parameters are arranged as follows: focal position 3 or 4, grayscale mapping 1 or 2, pseudo color 3 or 4, dynamic range 115 or 116, scanning range 0 or 1, scanning density, noise suppression 1 or 2, frame correlation 2 or 3, line average 2 or 3, space composite off, edge enhancement 0 or 1, image enhancement 0 or 1; S3. Observe during the inspection process, store and record the corresponding test results, and eliminate chickens with invisible tissue abnormalities or lesions based on the test results.

2. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 1, characterized in that: The detection parameters are set as follows: focus position 3, grayscale mapping 1, pseudo color 3, dynamic range 115, scanning range 0, scanning density medium, noise suppression 1, frame correlation 2, line average 2, spatial composite off, edge enhancement 0, image enhancement 0.

3. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 1, characterized in that: When testing the liver, the probe acts on the left and right parts of the skin from the keel ridge to the side edge of the hen; when testing the abdominal cavity, the probe acts on the left, middle and right parts of the skin at the end of the keel process of the hen; when testing the ovary or fallopian tube, the probe acts on the skin from the femoral head on the anatomical right side of the hen to the cloaca.

4. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 1, characterized in that: The Doppler ultrasonic diagnostic instrument is a DCU10 digital Doppler ultrasonic diagnostic instrument; the probes used by the Doppler ultrasonic diagnostic instrument during the detection process include a phased array probe and a linear array probe; the phased array probe has F=8.0MHz, D=60mm; the linear array probe has F=9.0MHz, D=40mm.

5. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 3, characterized in that: During the detection process, the phased array probe is gently placed and the transverse up and down swing scanning method is used to observe the left and right lobes of the liver. The linear array probe is gently placed and the sequential continuous parallel section method is used to observe the abdominal cavity. The three-dimensional fan-shaped section method is used to observe the ovaries or fallopian tubes through the phased array probe as the skin elasticity is evenly applied.

6. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 1, characterized in that: In step S3, the B model is used for observation. After clear sound and image are observed, they are stored as pictures in TIFF format and as videos in AVI format. The liver, abdominal cavity, and / or ovary, and / or oviduct of the chicken are observed through the video to determine the abnormality or pathological condition of the chicken tissue.

7. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 5, characterized in that: The criteria for judging liver abnormality or lesions are as follows: using a Doppler ultrasound diagnostic apparatus to observe that the ultrasound acoustic image characteristics of the left and right lobes of the liver are irregular and scattered high-echo areas, blurred edges of low-echo areas, occasional anechoic lesions, and uneven parenchymal echoes.

8. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 5, characterized in that: The criteria for judging the abdominal abnormality or lesion are: small or medium-sized abdominal effusion or cecal distension, reticular fiber separation of fluid areas, and jelly-like punctate high echoes.

9. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 5, characterized in that: The criteria for judging ovarian abnormality or pathology are: follicles lose high and low echo nested structures, are oval in shape, contain large dark areas, and have strong heterogeneous echoes distributed between follicles.

10. The method for early elimination of chickens with invisible tissue abnormalities or lesions according to claim 5, characterized in that: The fallopian tube abnormality or lesion is judged through the egg formation process, and the judgment criteria are divided into ampulla to isthmus lesions or fimbria development discordance or ovarian development discordance; among them, the judgment criteria for ampulla to isthmus lesions are: the yolk is degenerated and the central part of the egg is homogeneous and highly echoic and scattered, and mixed with anechoic areas; the judgment criteria for fimbria development discordance are: multiple eggs appearing in the eggshell gland at the same time; the judgment criteria for ovarian development discordance are: irregular follicle ultrasound images.

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