Orthosulfamuron hapten, artificial antigen and antibody as well as preparation methods and application of orthosulfamuron hapten, artificial antigen and antibody

By designing and synthetic oligine sulfuron semi -antigen and carrier protein, prepare more specific and higher -sensitivity antibodies, solve the long period, strong professionalism, strong professionalism, strong professionalism, strong professionalism, and strong professionalism in existing technologies. The high cost problem achieves a fast, sensitive and high -specific detection effect.

CN119330889AActive Publication Date: 2025-01-21SHENZHEN HAIJIXING AGRI PROD TESTING SCI & TECH CENT
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Patent Information

Application Number
CN202411512811.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-28
Publication Date
2025-01-21
Estimated Expiration
2044-10-28

AI Technical Summary

Technical Problem

The method for detecting pyrinilinesulfuron residues in the prior art has a long cycle, strong professionalism, high cost, and is not suitable for rapid on-site large-scale detection, and lacks pyrinilinesulfuron hapten, artificial antigen, and antibodies.

Method used

Design and synthesize a semi -antigen antigen, which is the carboxyl active group with a certain length interval arm by introducing the benzine sulfonon benzen in a sulfuron, so that it can be coupled with the protein of the carrier and obtain an artificial antigen for immunity. So as to prepare antibodies with stronger specificity and higher sensitivity.

Benefits of technology

The rapid, sensitive and specific detection of pyrimidinesulfuron is achieved, which reduces the detection cost and time, improves the applicability and efficiency of the detection, and is not interfered by structural analogs, reducing false positives or false negatives.

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Abstract

The invention discloses a orthosulfamuron hapten, an artificial antigen, an antibody and a preparation method and application thereof. The structure of the orthosulfamuron hapten is shown as a formula (I), a carboxyl active group with a certain length spacer arm is introduced to a benzene ring of orthosulfamuron to obtain the hapten, so that the hapten can be coupled with carrier protein to obtain an artificial antigen for immunization; the orthosulfamuron hapten reserves all characteristic groups of orthosulfamuron, the original structural characteristics of orthosulfamuron are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. According to the orthosulfamuron monoclonal antibody and indirect competitive ELISA based on the orthosulfamuron monoclonal antibody, the lowest detection limit of orthosulfamuron is 2.30 ng / mL, the IC50 is 17.65 ng / mL, the linear range is 4.87-63.94 ng / mL, the detection sensitivity is high, and the linear range is wide.
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Citation Information

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