Morchella oil based on supercritical carbon dioxide extraction and preparation method thereof

Abstract

The invention relates to the technical field of morchella oil extraction methods, in particular to morchella oil based on supercritical carbon dioxide extraction and a preparation method thereof.According to the morchella oil based on supercritical carbon dioxide extraction, the supercritical carbon dioxide extraction technology is adopted, grease can be efficiently extracted from morchella stems at the low temperature and pressure, and the morchella oil can be obtained. The damage of high temperature to nutritional ingredients can be effectively avoided, so that active ingredients and natural flavor in the morchella oil are reserved, and the nutritional value and quality of the product are improved; through the careful selecting, cleaning, drying and crushing steps, the purity and uniformity of morchella stem powder can be ensured, a good raw material basis is provided for subsequent supercritical carbon dioxide extraction, and particularly, surface impurities and excessive water can be effectively removed through deionized water flushing and electrothermal forced air drying; the morchella oil prepared in the invention can provide antioxidant components, delay senescence, provide fiber components and promote intestinal health, is suitable for all ages, and has excellent market promotion value.

Description

Technical Field

[0001] The invention relates to the technical field of morel oil extraction methods, and in particular to morel oil based on supercritical carbon dioxide extraction and a preparation method thereof. Background Art

[0002] Critical carbon dioxide refers to carbon dioxide whose temperature and pressure exceed its critical point. It has the viscosity and diffusion capacity of gas and the density and solubility of liquid. It has strong selectivity for non-polar solutes and is very suitable for extracting oil from oil. This method has the advantages of mild operating conditions, high oil extraction rate, good crude oil quality, no solvent residue, reduced loss of oil and fat accompaniments, and minimal pollution. It is a green edible vegetable oil processing method. The extraction rate of Morchella oil extracted by supercritical carbon dioxide extraction will be improved, and the product quality is excellent. It is a green and efficient oil extraction technology.

[0003] At present, most of the existing methods for preparing morel oil use traditional hot extraction methods. Traditional hot extraction methods usually require higher temperatures to extract the oil and flavor components in morels. However, high temperatures may cause certain nutrients in morels to be destroyed or lost, thereby affecting the nutritional value and quality of the final product. If supercritical carbon dioxide is used to extract morel oil, the nutritional value of morel oil can be further retained and the quality of morel oil can be improved. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a morel oil based on supercritical carbon dioxide extraction and a preparation method thereof, which can effectively solve the problem that the extraction method of the prior art cannot retain the nutritional components of the morel oil to the maximum extent and cannot enhance the flavor of the morel oil.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A method for preparing morel oil based on supercritical carbon dioxide extraction, the preparation method comprising the following steps:

[0009] Step 1, select, clean and dry the morel stems and crush them into morel stem powder;

[0010] Step 2, immersing the Morchella stem powder in vegetable oil, and pressing the mixture through a mesh filter press to obtain the Morchella stem coarse powder;

[0011] Step 3, extracting the Morchella stalk powder with supercritical carbon dioxide, and the obtained product is recorded as Morchella primary oil;

[0012] Step 4, centrifuge the morel primary oil to obtain morel oil.

[0013] Furthermore, the preparation steps of the Morchella stem powder in Step 1 are:

[0014] Step 1, first manually select the gravel impurities in the morel stem, then sieve with a 20-50 mesh separation sieve to remove grass clippings and morel stem debris, then immerse in 45°C distilled water for 5 minutes, stir at a speed of 50r / min for 10 minutes to remove the surface mud, then remove and rinse with deionized water 3 times;

[0015] Step 2, lay the Morchella stalk processed in step 1 on absorbent paper, lay another layer of absorbent paper on the surface and gently press dry the water, and finally place it in an electric hot air drying oven at 35° C. for 6-8 hours until the moisture content is less than 12%;

[0016] Step 3: crush the Morchella stalks processed in step 2 using a graded continuous feeding crusher, and the Morchella stalk powder is obtained after passing through a 30-mesh sieve.

[0017] Furthermore, the vegetable oil in Step 2 is one of corn oil, walnut oil and olive oil, and the material-liquid ratio of the impregnation in Step 2 is 1:1.3-1.5 g / mL.

[0018] Furthermore, the specific immersion method in Step 2 is: stirring at a stirring speed of 100 r / min for 5 minutes, then placing in a cold storage at 4° C. and immersing for 48 hours, and stirring 2-3 times during the immersion process.

[0019] Furthermore, in Step 3, the extraction temperature of the supercritical carbon dioxide extraction is 45°C, the extraction separation temperature is 50°C, the extraction pressure is 32Mpa, and the extraction time is 6h.

[0020] Furthermore, the specific operation of centrifugation in Step 4 is: centrifugation at a temperature of 25° C. and a speed of 8000 r / min for 5-6 minutes.

[0021] A morel oil based on supercritical carbon dioxide extraction is prepared by a preparation method of the morel oil based on supercritical carbon dioxide extraction.

[0022] Beneficial Effects

[0023] The present invention provides a morel oil based on supercritical carbon dioxide extraction and a preparation method thereof. Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention adopts supercritical carbon dioxide extraction technology, which can efficiently extract oil from morel stems at relatively low temperature and pressure, and can effectively avoid the destruction of nutrients by high temperature, thereby retaining the active ingredients and natural flavor in the morel oil, and improving the nutritional value and quality of the product; through the meticulous selection, cleaning, drying and crushing steps, the purity and uniformity of the morel stem powder can be ensured, providing a good raw material basis for subsequent supercritical carbon dioxide extraction, especially using deionized water washing and electric heating blast drying, which can effectively remove surface impurities and excess moisture, and ensure the extraction efficiency.

[0025] The invention selects suitable vegetable oil as the impregnation medium, and accurately controls the material-liquid ratio, stirring speed, impregnation time and temperature, which is helpful to better dissolve and release the oil and flavor components in the morel stem, thereby improving the yield and quality of the morel oil; the supercritical carbon dioxide extraction technology has the characteristics of being pollution-free and easy to recycle, and no chemical solvent needs to be added during the extraction process, which meets the requirements of green production, and at the same time, by accurately controlling the extraction conditions, energy consumption and production costs can be reduced; the morel oil after centrifugal treatment has impurities and sediments removed, and the clarity and stability of the product can be improved. The finally prepared morel oil has a strong natural flavor and unique nutritional value, and is suitable for the development of various foods and health products.

[0026] The morel oil prepared in the present invention can provide antioxidant components, delay aging, and can also provide fiber components to promote intestinal health. It is suitable for people of all ages and has excellent market promotion value. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The present invention will be further described below in conjunction with the embodiments.

[0029] Example 1

[0030] The present embodiment provides a method for preparing Morchella oil based on supercritical carbon dioxide extraction, the preparation method comprising the following steps:

[0031] Step 1, select, clean and dry the morel stems and crush them into morel stem powder;

[0032] Step 2, immersing the Morchella stem powder in vegetable oil, and pressing the mixture through a mesh filter press to obtain the Morchella stem coarse powder;

[0033] Step 3, extracting the Morchella stalk powder with supercritical carbon dioxide, and the obtained product is recorded as Morchella primary oil;

[0034] Step 4, centrifuge the morel primary oil to obtain morel oil.

[0035] The preparation steps of the Morchella stalk powder in Step 1 are as follows:

[0036] Step 1: first manually select the gravel impurities in the morel stem, then sieve with a 20-mesh separation sieve to remove grass debris and morel stem debris, then immerse in 45°C distilled water for 5 minutes, stir at a speed of 50r / min for 10 minutes to remove the surface mud, then remove and rinse with deionized water for 3 times;

[0037] Step 2, lay the Morchella stem processed in step 1 on absorbent paper, lay another layer of absorbent paper on the surface and gently press dry the water, and finally place it in an electric hot air drying oven at 35° C. for 6 hours until the moisture content is less than 12%;

[0038] Step 3: crush the Morchella stalks processed in step 2 using a graded continuous feeding crusher, and the Morchella stalk powder is obtained after passing through a 30-mesh sieve.

[0039] The vegetable oil in Step 2 is corn oil, and the material-liquid ratio of the immersion in Step 2 is 1:1.3 g / mL.

[0040] The specific immersion method in Step 2 is: stir at a stirring speed of 100 r / min for 5 minutes, then place in a cold storage at 4°C and immerse for 48 hours, turning it over twice during the immersion process.

[0041] In Step 3, the extraction temperature of supercritical carbon dioxide extraction is 45°C, the extraction separation temperature is 50°C, the extraction pressure is 32Mpa, and the extraction time is 6h.

[0042] The specific operation of centrifugation in Step 4 is: centrifuge at a temperature of 25°C and a speed of 8000r / min for 5 minutes.

[0043] A morel oil based on supercritical carbon dioxide extraction is prepared by a preparation method of the morel oil based on supercritical carbon dioxide extraction.

[0044] Example 2

[0045] The present embodiment provides a method for preparing Morchella oil based on supercritical carbon dioxide extraction, the preparation method comprising the following steps:

[0046] Step 1, select, clean and dry the morel stems and crush them into morel stem powder;

[0047] Step 2, immersing the Morchella stem powder in vegetable oil, and pressing the mixture through a mesh filter press to obtain the Morchella stem coarse powder;

[0048] Step 3, extracting the Morchella stalk powder with supercritical carbon dioxide, and the obtained product is recorded as Morchella primary oil;

[0049] Step 4, centrifuge the morel primary oil to obtain morel oil.

[0050] The preparation steps of the Morchella stalk powder in Step 1 are as follows:

[0051] Step 1: first manually select the gravel impurities in the morel stem, then sieve with a 50-mesh separation sieve to remove grass debris and morel stem debris, then immerse in 45°C distilled water for 5 minutes, stir at a speed of 50 r / min for 10 minutes to remove the surface mud, then remove and rinse with deionized water for 3 times;

[0052] Step 2, lay the Morchella stalk processed in step 1 on absorbent paper, lay another layer of absorbent paper on the surface and gently press dry the water, and finally place it in an electric hot air drying oven at 35° C. for 8 hours until the moisture content is less than 12%;

[0053] Step 3: crush the Morchella stalks processed in step 2 using a graded continuous feeding crusher, and the Morchella stalk powder is obtained after passing through a 30-mesh sieve.

[0054] The vegetable oil in Step 2 is walnut oil, and the material-liquid ratio of the immersion in Step 2 is 1:1.5 g / mL.

[0055] The specific immersion method in Step 2 is: stir at a stirring speed of 100 r / min for 5 minutes, then place in a cold storage at 4°C and immerse for 48 hours, turning over 3 times during the immersion process.

[0056] In Step 3, the extraction temperature of supercritical carbon dioxide extraction is 45°C, the extraction separation temperature is 50°C, the extraction pressure is 32Mpa, and the extraction time is 6h.

[0057] The specific operation of centrifugation in Step 4 is: centrifuge at a temperature of 25°C and a speed of 8000r / min for 6 minutes.

[0058] A morel oil based on supercritical carbon dioxide extraction is prepared by a preparation method of the morel oil based on supercritical carbon dioxide extraction.

[0059] Example 3

[0060] The present embodiment provides a method for preparing Morchella oil based on supercritical carbon dioxide extraction, the preparation method comprising the following steps:

[0061] Step 1, select, clean and dry the morel stems and crush them into morel stem powder;

[0062] Step 2, immersing the Morchella stem powder in vegetable oil, and pressing the mixture through a mesh filter press to obtain the Morchella stem coarse powder;

[0063] Step 3, extracting the Morchella stalk powder with supercritical carbon dioxide, and the obtained product is recorded as Morchella primary oil;

[0064] Step 4, centrifuge the morel primary oil to obtain morel oil.

[0065] The preparation steps of the Morchella stalk powder in Step 1 are as follows:

[0066] Step 1: first manually select the gravel impurities in the Morchella stalk, then sieve with a 30-mesh separation sieve to remove grass clippings and Morchella stalk debris, then immerse in 45°C distilled water for 5 minutes, stir at a speed of 50 r / min for 10 minutes to remove the surface mud, then remove and rinse with deionized water for 3 times;

[0067] Step 2, lay the Morchella stalk processed in step 1 on absorbent paper, lay another layer of absorbent paper on the surface and gently press dry the water, and finally place it in an electric hot air drying oven at 35° C. for 7 hours until the moisture content is less than 12%;

[0068] Step 3: crush the Morchella stalks processed in step 2 using a graded continuous feeding crusher, and the Morchella stalk powder is obtained after passing through a 30-mesh sieve.

[0069] The vegetable oil in Step 2 is olive oil, and the material-liquid ratio of the immersion in Step 2 is 1:1.43 g / mL.

[0070] The specific immersion method in Step 2 is: stir at a stirring speed of 100 r / min for 5 minutes, then place in a cold storage at 4°C and immerse for 48 hours, turning over 3 times during the immersion process.

[0071] In Step 3, the extraction temperature of supercritical carbon dioxide extraction is 45°C, the extraction separation temperature is 50°C, the extraction pressure is 32Mpa, and the extraction time is 6h.

[0072] The specific operation of centrifugation in Step 4 is: centrifuge at a temperature of 25°C and a speed of 8000r / min for 5 minutes.

[0073] A morel oil based on supercritical carbon dioxide extraction is prepared by a preparation method of the morel oil based on supercritical carbon dioxide extraction.

[0074] Performance Testing

[0075] The Morchella oils prepared in Examples 1-3 based on supercritical carbon dioxide extraction were marked as Example 1, Example 2 and Example 3, respectively, and then the performance of Examples 1-3 was tested. The specific testing methods and test items are as follows:

[0076] 1. Organize a sensory evaluation team with 15 members by using the sensory evaluation method. After standard training to keep the psychological scale of the tasters constant, conduct a sensory evaluation experiment for two weeks to perform sensory evaluation on Examples 1-3. The specific scoring criteria are shown in Table 1, and the obtained data are recorded in Table 2.

[0077] 2. Taking Example 1 as an example, the morel oil was analyzed by gas phase-ion mobility spectrometry, and the obtained data are recorded in Table 3;

[0078] 3. Taking Example 1 as an example, the health indicators of Morchella oil were analyzed, and the obtained data were recorded in Table 4;

[0079] Table 1

[0080]

[0081]

[0082] Table 2

[0083] Example 1 Example 2 Example 2 Overall Rating 78 79 78

[0084] Table 3

[0085]

[0086]

[0087] Table 4

[0088]

[0089] It can be seen from the data in the above table that the morel oil prepared by the preparation method of morel oil based on supercritical carbon dioxide extraction in the present invention contains more kinds of substances, has good oil flavor quality, and the hygiene indicators meet the national standards, and has excellent market promotion value.

[0090] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0091] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing morel oil based on supercritical carbon dioxide extraction, characterized in that, The preparation method comprises the following steps: Step 1, select, clean and dry the morel stems and crush them into morel stem powder; Step 2, immersing the Morchella stem powder in vegetable oil, and pressing the mixture through a mesh filter press to obtain the Morchella stem coarse powder; Step 3, extracting the Morchella stalk powder with supercritical carbon dioxide, and the obtained product is recorded as Morchella primary oil; Step 4, centrifuge the morel primary oil to obtain morel oil.

2. The method for preparing morel oil based on supercritical carbon dioxide extraction according to claim 1, characterized in that: The preparation steps of Morchella stalk powder in Step 1 are as follows: Step 1, first manually select the gravel impurities in the morel stem, then sieve with a 20-50 mesh separation sieve to remove grass clippings and morel stem debris, then immerse in 45°C distilled water for 5 minutes, stir at a speed of 50r / min for 10 minutes to remove the surface mud, then remove and rinse with deionized water 3 times; Step 2, lay the Morchella stalk processed in step 1 on absorbent paper, lay another layer of absorbent paper on the surface and gently press dry the water, and finally place it in an electric hot air drying oven at 35° C. for 6-8 hours until the moisture content is less than 12%; Step 3: crush the Morchella stalks processed in step 2 using a graded continuous feeding crusher, and the Morchella stalk powder is obtained after passing through a 30-mesh sieve.

3. The method for preparing morel oil based on supercritical carbon dioxide extraction according to claim 1, characterized in that: The vegetable oil in Step 2 is one of corn oil, walnut oil and olive oil, and the material-liquid ratio of the immersion in Step 2 is 1:1.3-1.5 g / mL.

4. The method for preparing morel oil based on supercritical carbon dioxide extraction according to claim 1, characterized in that: The specific immersion method in Step 2 is: stir at a stirring speed of 100 r / min for 5 minutes, then place in a cold storage at 4°C and immerse for 48 hours, stirring 2-3 times during the immersion process.

5. The method for preparing morel oil based on supercritical carbon dioxide extraction according to claim 1, characterized in that: In Step 3, the extraction temperature of supercritical carbon dioxide extraction is 45°C, the extraction separation temperature is 50°C, the extraction pressure is 32Mpa, and the extraction time is 6h.

6. The method for preparing morel oil based on supercritical carbon dioxide extraction according to claim 1, characterized in that: The specific operation of centrifugation in Step 4 is: centrifuge at a speed of 8000r / min for 5-6min at a temperature of 25°C.

7. A morel oil based on supercritical carbon dioxide extraction, characterized in that: The oil is prepared by the method for preparing morel oil based on supercritical carbon dioxide extraction as described in any one of claims 1 to 6.

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