EpCYS gene of scrophularia sinensis scpl family acyltransferase and application thereof

By cloning and expressing the EpCYS gene of the SCPL family acyltransferase of Echinacea purpurea, yeast and plant expression vectors were constructed, achieving efficient conversion of chlorogenic acid to cynarine. This solves the problem of low catalytic efficiency in existing technologies and promotes the application of cynarine in pharmaceutical components.

CN119331891BActive Publication Date: 2026-06-09SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN UNIV
Filing Date
2024-12-06
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing technologies are insufficient for efficiently catalyzing the formation of cynarine from chlorogenic acid, which limits the biosynthesis and application of cynarine and its derivatives in pharmaceutical formulations.

Method used

The gene of EpCYS, an acyltransferase of the SCPL family from Echinacea purpurea, was cloned and expressed. Yeast and plant expression vectors were constructed to achieve heterologous expression of EpCYS in yeast and Nicotiana benthamiana, which catalyzes the production of cynarine from chlorogenic acid.

Benefits of technology

The successful in vitro and in vivo validation of EpCYS-catalyzed production of cynarine from chlorogenic acid provides a new resource for the biosynthesis of cynarine, promotes its production as a drug component, and demonstrates its biological activities such as hepatoprotection, blood sugar regulation, antibacterial and anti-inflammatory effects, and inhibition of cancer cells.

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Abstract

The application discloses a SCPL family acyltransferase EpCYS gene of purple Echinacea purpurea, an amino acid sequence encoded by the EpCYS gene is shown as SEQ ID NO. 2, the gene is heterologously expressed in yeast or Nicotiana benthamiana, and after the expressed protein is used to catalyze a substrate in vitro, the catalyzed product is identified; it is shown that the EpCYS protease can catalyze the generation of Cynarine; more resources are provided for the application of plant acyltransferase in synthetic biology, the biosynthesis production of Cynarine as a medicinal ingredient is promoted, and a new contribution will be made for human health.
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