A formed-component imaging system

Abstract

A formed element imaging system, which includes a liquid injection mechanism, a detection mechanism, and a flow cell assembly. The liquid injection mechanism includes a solenoid valve unit, a syringe unit, and a motor assembly. The syringe unit includes a cleaning pump assembly, a sheath fluid pump assembly, a sample laminar flow pump assembly, and a sampling pump assembly. The detection mechanism includes a substrate, a flow cell assembly, an illumination module, and an imaging module. The flow cell assembly includes a flow cell bracket and a flow cell. By connecting the solenoid valve unit to the syringe unit, a stable laminar flow effect can be formed for the sample in the flow channel. The flow cell made of colorless high-transparency glass by integral molding avoids the influence of airtightness and light transmittance problems on imaging. The thickness of the laminar flow is limited to 4 ± 0.5 microns, so that the characteristic surface of the formed element can face the imaging module directly, ensuring that the imaging module can capture high-definition images.

Description

Technical Field

[0001] The present invention relates to the technical field of medical diagnostic devices, and in particular to a formed element imaging system. Background Art

[0002] Currently, in all domestic and foreign medical fields, there are only two technologies for automatic urine detection devices, namely: planar flow technology and urine sedimentation detection technology.

[0003] The existing detection technologies have the following problems:

[0004] 1. Due to control differences, both technologies have unclear imaging situations.

[0005] 2. The essence of the sedimentation method is to centrifuge the liquid, that is, there will be a phenomenon of formed element superposition. Because various formed elements overlap each other, the accuracy rate of photographing and recognition is greatly reduced.

[0006] 3. The types of formed elements that the sedimentation method can detect are limited, all below 9 types.

[0007] 4. Due to the defect of the control method in the planar flow technology, the laminar flow is unstable, and the imaging is sometimes clear and sometimes blurred.

[0008] 5. Problems with the raw materials of the flow cell used in the planar flow technology (specifically including the selection of colored materials resulting in low light transmittance, and the adhesive process problem causing the flow channel of the flow cell to be unsealed, thus leading to unstable laminar flow), resulting in blurred imaging and inability to identify formed elements.

[0009] In Chinese Patent CN2018112976713, a flow cell mechanism adjustment device for a fully automatic urine formed element analyzer is disclosed. It is composed of a motor base plate, an optocoupler bracket, a bearing seat, a flanged bearing, an optocoupler holder, a lateral adjustment plate, a lateral rotating block, a lining plate pressing block, a flow cell positioning cover, a flow cell, a silica gel pad, a flow cell lining plate, etc., and is driven by a stepping motor, with a lead screw drive with a backlash nut (to eliminate backlash), and cooperates with a linear guide rail to control the movement of the flow cell along the optical axis direction and automatically find the focus of the optical system. It itself cannot eliminate the defects of the flow cell itself, that is, low light transmittance of the flow cell, poor imaging, unstable laminar flow, etc. Summary of the Invention

[0010] In view of this, the present invention provides a formed element imaging system to solve the above technical problems.

[0011] A formed - element imaging system includes a frame, a liquid injection mechanism, a detection mechanism, and a flow cell assembly disposed in the detection mechanism. The detection mechanism includes an imaging module, and the flow cell assembly includes a flow cell and a sample nozzle disposed on the flow cell. The flow cell includes a flow cell body, a laminar liquid flow channel disposed in the flow cell body, a sheath liquid inlet disposed on the flow cell body and communicating with the laminar liquid flow channel, and an outlet disposed on the flow cell body and communicating with one end of the laminar liquid flow channel. The laminar liquid flow channel communicates with the sample nozzle and the sheath liquid inlet. The sheath liquid inlet and the sample nozzle are disposed at the same end of the laminar liquid flow channel. In a cross - section along the extending direction of the laminar liquid flow channel, the contour of the laminar liquid flow channel is a gradually - shrinking arc. In a cross - section perpendicular to the extending direction of the laminar liquid flow channel, the contour of the laminar liquid flow channel is an ellipse. The sample nozzle includes an insertion pipe, a nozzle base body disposed at one end of the insertion pipe, a sample inlet disposed on the nozzle base body and communicating with the insertion pipe, and a flat nozzle disposed at the end of the insertion pipe away from the nozzle base body. The sheath liquid inlet and the sample nozzle are both disposed at the same end of the laminar liquid flow channel. The flat nozzle makes the sample liquid be in a flat shape when sprayed into the laminar liquid flow channel, and the flat nozzle is located at the center of the laminar liquid flow channel. The width of the laminar liquid flow channel accommodating the flat nozzle is greater than the width of the other end of the laminar liquid flow channel, so that a gradually - shrinking section is formed in the middle region of the laminar liquid flow channel to guide the flow direction of the sheath liquid, and the gradually - shrinking section faces the flat nozzle so that the sample liquid can be wrapped by the sheath liquid flowing down from the two side walls of the gradually - shrinking section after being ejected. The flat nozzle is parallel to the long axis of the laminar liquid flow channel so that the sample liquid flowing out of the flat nozzle can directly flow into the space between the two side walls of the gradually - shrinking section. In the direction of the sample flow, the position of the outlet of the sheath liquid nozzle is higher than the position of the outlet of the flat nozzle. Driven by the liquid injection mechanism, the flow rate range of the sheath liquid is between 128 uL / s and 146 uL / s, and the flow rate ratio between the sheath liquid and the sample liquid is between 31.4 and 35.8 to stratify the sample. After the sample is stratified, the imaging module images the sample to obtain a clear image.

[0012] Further, the frame includes a syringe mounting rack and four syringe plates arranged on the syringe mounting rack.

[0013] Further, the syringe mounting rack is integrally in an "L" shape, and its short - side part is a solenoid valve mounting part.

[0014] Further, each of the syringe plates includes a syringe plate body, a sliding hole provided at one end of the syringe plate body away from the solenoid valve mounting portion, and a bottom plate groove provided at an end of the syringe plate body close to the sliding hole.

[0015] Further, the liquid injection mechanism includes a solenoid valve unit provided on the solenoid valve mounting portion, a syringe unit provided on the syringe plate, and four motor assemblies provided on the end face of the syringe plate facing away from the syringe unit. The liquid injection mechanism includes a solenoid valve unit provided on the solenoid valve mounting portion, a syringe unit provided on the syringe mounting plate, and four motor assemblies provided on the end face of the syringe mounting plate facing away from the syringe unit.

[0016] Further, the solenoid valve unit includes a first solenoid valve, a second solenoid valve, a third solenoid valve, a fourth solenoid valve, a fifth solenoid valve, a sixth solenoid valve, a seventh solenoid valve, and an eighth solenoid valve arranged in sequence from left to right.

[0017] Further, the syringe unit includes a cleaning pump assembly, a sheath fluid pump assembly, a sample flow pump assembly, a sampling pump assembly, and a sampling needle arranged on the frame from right to left. The sampling needle is connected to the sampling pump assembly and the first solenoid valve.

[0018] Further, the cleaning pump assembly, the sheath fluid pump assembly, the sample flow pump assembly, and the sampling pump assembly have the same structure, and each of them includes a syringe body and a push rod provided at an end of the syringe body away from the solenoid valve.

[0019] Further, each of the motor assemblies includes a motor mounting plate on the frame, a bottom plate provided at one end of the frame, a linear motor fixedly provided on the motor mounting plate, at least two guide shafts respectively provided at both ends of the linear motor and the bottom plate, and a slider having one end connected to the movable end of the linear motor and movably provided on the guide shafts.

[0020] Further, an exhaust port is further provided at one end of the laminar liquid flow channel close to the sheath fluid inlet.

[0021] Further, a breathable and waterproof membrane is provided at the exhaust port.

[0022] Compared with the prior art, a formed element imaging system provided by the present invention sucks a sample into the sample laminar flow pump assembly through the sampling needle. The solenoid valve unit is connected to the syringe unit, so that after computational fluid dynamics, the operation speeds of the sample laminar flow pump assembly and the sheath fluid pump assembly can be precisely controlled through the motor assembly, thereby controlling the sample flow rate and the sheath fluid flow rate, enabling the sample to form a stable laminar flow effect in the flow channel and avoiding the superposition of formed elements. Moreover, the flow cell integrally formed with colorless and highly transparent glass has the effects of high light transmittance and high airtightness, avoiding the influence of airtightness and light transmittance problems on imaging. And the thickness of the laminar flow is limited to 4 ± 0.5 microns, which is much smaller than the 8-micron diameter of the formed element, enabling the characteristic surface of the formed element to face the camera and pass through the flow channel along with the laminar flow, ensuring that the imaging module can capture the formed elements flowing along with the laminar flow. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 FIG. is a schematic structural diagram of a formed element imaging system provided by the present invention.

[0024] Figure 2 is Figure 1 a schematic structural diagram of the formed element imaging system from another perspective.

[0025] Figure 3 is Figure 1 a schematic structural diagram of the syringe plate of the formed element imaging system.

[0026] Figure 4 is Figure 1 a schematic structural diagram of the syringe unit of the formed element imaging system.

[0027] Figure 5 is Figure 1 a schematic structural diagram of the flow cell of the formed element imaging system.

[0028] Figure 6 is Figure 5 a schematic cross-sectional structural diagram of the flow cell.

[0029] Figure 7 is Figure 6 a schematic cross-sectional structural diagram of the flow cell from another perspective. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following further details the specific embodiments of the present invention. It should be understood that the description of the embodiments of the present invention herein is not intended to limit the protection scope of the present invention.

[0031] As Figures 1 to 7As shown in the figure, it is a schematic structural diagram of a tangible component imaging system provided by the present invention. The tangible component imaging system includes a frame 10, a liquid injection mechanism 20, a detection mechanism 30, and a flow cell assembly 40 disposed in the detection mechanism 30. It can be envisioned that the tangible component imaging system further includes some other functional structures, such as pipe assemblies, lead screw slides, etc., which are well-known technologies to those skilled in the art and will not be elaborated herein.

[0032] The frame 10 is fixedly disposed on a workbench (not shown in the figure) to provide support for the above functional modules. The frame 10 includes a syringe mounting bracket 11 and four syringe plates 12 arranged on the syringe mounting bracket 11. The syringe mounting bracket 11 is integrally in an "L" shape, and its short side portion is a solenoid valve mounting portion 13, providing space for mounting solenoid valves. The four syringe plates 12 are arranged on the syringe mounting bracket 11. Each syringe plate 12 includes a syringe plate main body 121, a sliding hole 122 provided at one end of the syringe plate main body 121 away from the solenoid valve mounting portion 13, and a bottom plate groove 123 provided at the end of the syringe plate main body 121 close to the sliding hole 122. The syringe plate 12 is used in cooperation with the liquid injection mechanism 20 and will be described in detail below in conjunction with the liquid injection mechanism 20.

[0033] The liquid injection mechanism 20 includes a solenoid valve unit 21 disposed on the solenoid valve mounting portion 13, a syringe unit 22 disposed on the syringe plate 12, and four motor assemblies 23 disposed on the end faces of the syringe plate 12 facing away from the syringe unit 22.

[0034] Please refer to Figure 1 , the solenoid valve unit 21 includes a first solenoid valve 211, a second solenoid valve 212, a third solenoid valve 213, a fourth solenoid valve 214, a fifth solenoid valve 215, a sixth solenoid valve 216, a seventh solenoid valve 217, and an eighth solenoid valve 218 arranged in sequence from left to right. And the first solenoid valve 211 is connected to the second solenoid valve 212 through a pipeline. The number of solenoid valves can be increased or decreased according to actual needs.

[0035] The solenoid valve unit 21 is connected to the syringe unit 22. The syringe unit 22 includes a cleaning pump assembly 221, a sheath fluid pump assembly 222, a sample flow pump assembly 223, a sampling pump assembly 224, and a sampling needle 227 that are arranged on the syringe plate 12 from right to left. The first solenoid valve 211, the second solenoid valve 212 are connected to the sampling pump assembly 224 and the sample flow pump assembly 223, enabling either the sampling pump assembly 224 or the sample flow pump assembly 223 to independently close one of the pipelines, facilitating the control of the liquid flow direction. The connecting pipeline between the first solenoid valve 211 and the sampling pump assembly 224 is also connected to the sampling needle 227, allowing the sampling pump assembly 224 to draw samples through the sampling needle 227 and transport them to the sample flow pump assembly 223. The cleaning pump assembly 221 and the third, fifth, sixth, seventh, and eighth solenoid valves 213, 215, 216, 217, 218 are all connected to a pipeline integration system (not shown in the figure) to achieve the transportation of different types of sample liquids, such as urine, blood, etc., avoiding sample contamination caused by multiple sample liquids sharing a single solenoid valve and for the purpose of cleaning the flow cell. Since this embodiment mainly illustrates the sampling of samples and the formation of a laminar flow with the sheath fluid, the third solenoid valve 213, the fifth solenoid valve 215, the sixth solenoid valve 216, the seventh solenoid valve 217, and the eighth solenoid valve 218 will not be further described.

[0036] The structures of the cleaning pump assembly 221, the sheath fluid pump assembly 222, the sample flow pump assembly 223, and the sampling pump assembly 224 are all the same. They each consist of a syringe body 225 and a push rod 226 arranged at the end of the syringe body 225 away from the solenoid valve unit 21. The solenoid valve unit 21 is connected to the syringe body 225 through a pipeline. The cleaning pump assembly 221 is used to pump cleaning liquid into the detection mechanism 30 for cleaning. The sheath fluid pump assembly 222 is used to pump sheath fluid into the detection mechanism 30, and the flow rate of the sheath fluid in the sheath fluid pump assembly 222 is adjusted through the fourth solenoid valve 214 and the motor assembly 23. The sample flow pump assembly 223 is used to pump sample liquid into the detection mechanism 30, and the flow rate of the sample in the sample flow pump assembly 223 is adjusted through the second solenoid valve 212 and the motor assembly 23. The sampling pump assembly 224 is used for sampling. The above four components all need to be used in conjunction with the motor assembly 23.

[0037] The number of the motor assemblies 23 corresponds to the number of components included in the syringe unit 22. Each of the motor assemblies 23 includes a motor mounting plate 231 on the frame 10, a bottom plate 232 provided at one end of the frame 10, a linear motor 233 fixedly provided on the motor mounting plate 231, at least two guide shafts 234 with both ends respectively provided on the linear motor 233 and the bottom plate 232, and a slider 235 with one end connected to the movable end of the linear motor 233 and movably provided on the guide shafts 234.

[0038] The movable end of the linear motor 233 is arranged towards the bottom plate 232, so as to drive the slider 235 to slide along the guide shafts 234. Two linear bearings 236 are arranged at intervals in the middle of the guide shafts 234. Both of the two linear bearings 236 protrude from the two end faces of the slider 235. The linear bearings 236 are of the prior art and will not be elaborated here. The slider 235 is slidably arranged on the guide shafts 234 through the two linear bearings 236, and the self-lubricating effect of the linear bearings 236 is used to reduce wear and improve the sliding smoothness. Each of the sliders 235 is connected to a push rod 226, and the push rod 226 is pushed by the linear motor 233 to push out the sample in the syringe body 225. Moreover, an operator can control the pushing speed of the push rod 226 by adjusting the speed of the linear motor 233, so as to adjust the flow rates of the sample and the sheath fluid.

[0039] When the syringe unit 22 and the motor assemblies 23 are in use, through hydrodynamic calculation, it is first ensured that the "Reynolds number (a term in fluid mechanics)" of the sheath fluid and the sample is less than 2300, so as to form a laminar flow between the sheath fluid and the sample liquid. Then, the speeds of the two linear motors 233 are adjusted to change the flow rates of the sheath fluid and the sample liquid, and further change the thickness of the laminar flow, that is, the laminar flow will become thinner when the flow rate becomes faster, and the laminar flow will become thicker when the flow rate becomes slower. If the flow rate of the sheath fluid is too fast, the thickness of the laminar flow will be too thin, and more light sources will penetrate through the flow cell assembly 40 and then irradiate on the imaging module 34, which is not conducive to focusing. If the flow rate of the sheath fluid is too slow, the laminar flow will become too thick, and the light source cannot completely penetrate the sheath fluid, causing the light to diffuse in the sheath fluid, which is not conducive to the imaging module 34 to capture the formed components in the sample liquid.

[0040] Therefore, it is necessary to control the flow rates between the sheath fluid and the sample fluid. The flow rate range of the sheath fluid is between 128 uL / s and 146 uL / s. The reason within this range is that only within this range can the sheath fluid flow down along the side wall of the following laminar flow channel 412 to wrap the sample. When it is less than 128 uL / s, it will aggregate into a mass due to the action of surface tension and cannot form a layer. When it is greater than 146 uL / s, it cannot adhere to the wall due to the too fast flow rate and thus forms a linear flow, and also cannot wrap the sample below. Specifically, when the sheath fluid flow rate is 128 uL / s and the sample flow rate is 4.08 uL / s, the laminar thickness is 4 microns. When the sample flow rate remains unchanged, when the sheath fluid flow rate is 114 uL / s, the laminar thickness is 4.5 microns, and when the sheath fluid flow rate is 146 uL / s, the laminar thickness is 3.5 microns. Therefore, in this embodiment, the flow rate of the sample fluid is maintained at 4.08 uL / s. Under the drive of the injection mechanism 20, the flow rate range of the sheath fluid is between 128 uL / s and 146 uL / s, and the flow rate ratio between the sheath fluid and the sample fluid is between 31.4 and 35.8 to stratify the sample, that is, the flow rate of the sheath fluid is 31.4 to 35.8 times that of the sample fluid. It can be imagined that while maintaining the flow rate ratio between the sheath fluid flow rate and the sample flow rate, the flow rates of the sheath fluid and the sample fluid can be scaled proportionally to suit devices with different sampling efficiencies.

[0041] The detection mechanism 30 includes a substrate 31, a flow cell bracket 32 arranged in the middle area of the substrate 31, an illumination module 33 arranged on the substrate 31 on one side of the flow cell bracket 32, and an imaging module 34 arranged on the substrate 31 on the other side of the flow cell bracket 32.

[0042] The substrate 31 is fixed on a plane such as a desktop or a workbench, and it is used to carry the above-mentioned various functional modules, such as the flow cell bracket 32, the illumination module 33, and the imaging module, etc.

[0043] The flow cell bracket 32 is used to clamp and fix the flow cell assembly 40, and at the same time, the position of the flow cell assembly 40 can also be adjusted. The flow cell bracket 32 includes a bracket base 321, a bracket 322 arranged on the bracket base 321, a clamping base 323 embedded in the bracket 322, and a clamping cover plate 324 covering the clamping base 323. The bracket 322 is vertically arranged on the bracket base 321. The clamping base 323 and the clamping cover plate 324 are arranged on the end face of the bracket 322 on the side facing away from the illumination module 33. A lighting hole 325 is opened at a position on the bracket 322 corresponding to the illumination module 33, so that the light source of the illumination module 33 can directly irradiate the flow cell bracket 32.

[0044] The lighting module 33 is an existing device on the market that provides a stable light source for sample detection, such as a laser or a flashing xenon lamp, etc. It is prior art and will not be elaborated here.

[0045] The imaging module 34 is used to capture images of the formed components in the sample under the light source for analysis. The imaging module 34 is made of heat-insulating material to prevent the optical unit from being affected by temperature.

[0046] The flow cell assembly 40 includes a flow cell 41, a sample nozzle 42 disposed on the flow cell 41, and a sheath liquid nozzle 43 disposed on the flow cell 41. The flow cell assembly 40 is placed in the clamping base 323, and the clamping cover plate 324 is covered on the flow cell assembly 40 and is screwed and fixed to the clamping base 323, thereby fixing the flow cell assembly 40 in the flow cell bracket 32.

[0047] The flow cell 41 includes a flow cell body 411, a laminar liquid flow channel 412 disposed in the flow cell body 411, a sheath liquid inlet 413 disposed on the flow cell body 411 and communicating with the laminar liquid flow channel, and an outlet 414 disposed on the flow cell body 411 and communicating with one end of the laminar liquid flow channel 412. The flow cell 41 itself is prior art, such as an inspection flow cell applied to urine analysis disclosed in CN118190928A.

[0048] The flow cell body 411 is made of colorless high-transparency glass and is manufactured by a melt-integrated processing method. Using colorless high-transparency glass can achieve the purpose of almost lossless light passing, ensuring the clarity of imaging. The melt-integrated processing method can also ensure the integrity and sealing of the structure, reduce the risk of leakage, and ensure the uniformity of the material, reducing imaging errors caused by material non-uniformity.

[0049] An exhaust port 415 is further provided at one end of the laminar liquid flow channel 412 close to the sheath liquid inlet 413.

[0050] The laminar liquid flow channel 412 is used to guide the flow of the detection sample. The laminar liquid flow channel 412 is in communication with the sample nozzle 42, the sheath liquid inlet 413, and the exhaust port 415. The sheath liquid inlet 413 and the sample nozzle 42 are provided at the same end of the laminar liquid flow channel 412. The sample nozzle 42 injects the sample liquid sample into the laminar liquid flow channel 412. The sheath liquid inlet 413 injects the sheath liquid into the laminar liquid flow channel 412. The exhaust port 415 discharges the excess air bubbles in the laminar liquid flow channel 412. In the cross-section along the extension direction of the laminar liquid flow channel 412, the profile of the laminar liquid flow channel 412 is a gradually shrinking arc. In the cross-section perpendicular to the extension direction of the laminar liquid flow channel 412, the profile of the laminar liquid flow channel 412 is an ellipse.

[0051] The sheath liquid inlet 413, the exhaust port 415, and the sample nozzle 42 are all provided at the same end of the laminar liquid flow channel 412. A breathable and waterproof film is provided at the exhaust port 415 to prevent liquid leakage. The liquid outlet 414 is provided at one end of the laminar liquid flow channel 412 facing away from the sample nozzle 42 to enable the entry and exit of the sample liquid sample.

[0052] The sample nozzle 42 is used to inject the sample liquid sample into the laminar liquid flow channel 412. The sample nozzle 42 includes an insertion tube 421, a nozzle base body 422 provided at one end of the insertion tube 421, a sample liquid inlet 423 provided on the nozzle base body 422 and communicating with the insertion tube 421, and a flat nozzle 424 provided at the end of the insertion tube 421 away from the nozzle base body 422.

[0053] When the sample nozzle 42 is fixed to the flow cell body 411, the insertion tube 421 is inserted into the laminar liquid flow channel 412. And the nozzle base body 422 and the flow cell body 411 are fixed and sealed with glue.

[0054] Please refer to Figure 7, the flat nozzle 424 makes the sample liquid flat when it is ejected into the laminar flow channel 412, and the flat nozzle 424 is located at the center of the laminar flow channel 412. The width of the laminar flow channel 412 accommodating the flat nozzle 424 is greater than the width of the other end of the laminar flow channel 412, so that a tapered section 416 is formed in the middle region of the laminar flow channel 412. The tapered section 416 can guide the flow direction of the sheath liquid, and the tapered section 416 is directly opposite to the flat nozzle 424, so that the sample liquid can be wrapped by the sheath liquid flowing down from both side walls of the tapered section 416 after being ejected. And the flat nozzle 424 is parallel to the long axis of the laminar flow channel 412, so that the sample liquid flowing out from the flat nozzle 424 can directly flow into the space between the two side walls of the tapered section 416.

[0055] One end of the sheath liquid nozzle 43 is arranged in the sheath liquid inlet 413. When the flow cell 41 is clamped on the flow cell bracket 32, in the direction of the sample flow, the position of the liquid outlet of the sheath liquid nozzle 43 is higher than the position of the liquid outlet of the flat nozzle 424, so that the sheath liquid can wrap the sample liquid when flowing in the laminar flow channel 412, that is, the sample liquid can be immediately wrapped by the sheath liquid after flowing out from the flat nozzle 424, so as to form a laminar flow earlier and increase the time for the subsequent imaging module 34 to take pictures.

[0056] The sheath liquid inlet 413 is connected to the second solenoid valve 212, and the sample nozzle 42 is communicated with the fourth solenoid valve 214 through a pipeline. Thus, the flow of the sample and the sheath liquid in the laminar flow channel 412 is controlled.

[0057] The above-mentioned sheath liquid is a balanced electrolyte solution without fluorescent background, which is mainly used in flow cytometers and some blood analyzers to improve the detection accuracy.

[0058] During use, the sample laminar flow pump assembly 223 is communicated with the sample nozzle 42, and the sheath liquid pump assembly 222 is communicated with the sheath liquid inlet 413. The two motor assemblies 23 respectively push the sample laminar flow pump assembly 223 and the sheath liquid pump assembly 222 to inject the sample and the sheath liquid into the laminar flow channel 412. At the same time, after hydrodynamic calculation, the second solenoid valve 212 and the fourth solenoid valve 214 respectively control the sample laminar flow pump assembly 223 and the sheath liquid pump assembly 222, so as to control the sample flow rate and the sheath liquid flow rate. At the same time, the flow rate of the sheath liquid should be greater than the flow rate of the sample, flowing down from both side walls of the tapered section 416 to ensure that the sheath liquid can surround the sample liquid and make the sample form a stable laminar flow effect in the laminar flow channel 412, that is, the sample liquid is in the center, the laminar flow thickness is 4 ± 0.5 microns, and the sheath liquid is wrapped on both sides.

[0059] Since the sample cells flow out of the sample nozzle 42 continuously and even overlappingly, in order for the camera to capture individual sample cells, the continuous and even overlapping sample cells must be separated. However, the separation speed cannot be too fast, otherwise the cells will be broken through or it will be difficult for the imaging module 34 to focus.

[0060] Therefore, the ratio of the speed of the sheath fluid to the speed of the sample must be controlled within a certain range, so that the faster-flowing sheath fluid can bring out individual sample cells in the slower-flowing sample one by one with a certain interval between them, achieving a layering effect. And when flowing through the laminar fluid flow channel 412, the imaging module 34 takes pictures. Therefore, when the flow rate range of the sheath fluid is between 128 uL / s and 146 uL / s, and the flow rate ratio between the sheath fluid and the sample liquid is between 31.4 and 35.8, that is, the flow rate of the sheath fluid is 31.4 to 35.8 times that of the sample liquid. If the flow rate of the sheath fluid is less than 128 uL / s and the flow rate ratio is less than 31.4, the sample cells cannot be taken out of the sample flow one by one at intervals. And if the flow rate of the sheath fluid is greater than 146 uL / s and the flow rate ratio is less than 35.8, it may break through the sample cells and the imaging cannot be accurately focused.

[0061] And since the formed elements in the sample (such as red blood cells, white blood cells, epithelial cells, etc.) have diameters of more than 8 micrometers, when the laminar thickness is less than the diameter of the formed elements, it can make the characteristic surface of the formed elements face the camera and pass through the flow cell channel, so that the imaging module 34 can take a clearer image for analysis.

[0062] Compared with the prior art, a formed element imaging system provided by the present invention sucks a sample into the sample laminar flow pump assembly 223 through the sampling needle 227. The solenoid valve unit 21 is connected to the syringe unit 22, so that after computational fluid dynamics, the running speeds of the sample laminar flow pump assembly 223 and the sheath fluid pump assembly 222 can be accurately controlled by the motor assembly 23, thereby controlling the sample flow rate and the sheath fluid flow rate, so that the sample can form a stable laminar flow effect in the laminar fluid flow channel 412, avoiding the superposition of formed elements. And the flow cell 41 made of colorless high-transparency glass integrally formed has the effects of high light transmittance and high airtightness, avoiding the problems of airtightness and light transmittance affecting imaging. And the thickness of the laminar flow is limited to 4 ± 0.5 micrometers, which is much smaller than the 8-micrometer diameter of the formed elements, so that the characteristic surface of the formed elements can face the camera and pass through the laminar fluid flow channel 412 along with the laminar flow, ensuring that the imaging module 34 can capture the formed elements flowing along with the laminar flow.

[0063] The above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements or improvements within the spirit of the present invention are all covered within the scope of the claims of the present invention.

Claims

1. A shaped component imaging system, characterized in that: The formed component imaging system comprises a frame, a liquid injection mechanism, a detection mechanism, and a flow cell assembly arranged in the detection mechanism, wherein the detection mechanism comprises an imaging module, the flow cell assembly comprises a flow cell, and a sample nozzle arranged on the flow cell, the flow cell comprises a flow cell body, a laminar flow channel arranged in the flow cell body, a sheath liquid inlet arranged on the flow cell body and connected to the laminar flow channel, and a liquid outlet arranged on the flow cell body and connected to one end of the laminar flow channel, wherein the The laminar liquid flow channel is connected with the sample nozzle and the sheath liquid inlet. The sheath liquid inlet and the sample nozzle are arranged at the same end of the laminar liquid flow channel. On the cross section along the extension direction of the laminar liquid flow channel, the profile of the laminar liquid flow channel is a tapered arc. On the cross section perpendicular to the extension direction of the laminar liquid flow channel, the profile of the laminar liquid flow channel is an ellipse. The sample nozzle includes a plug-in tube, a nozzle base body arranged at one end of the plug-in tube, a sample inlet arranged on the nozzle base body and connected to the plug-in tube, and a nozzle base body arranged at a distance from the plug-in tube. The flat nozzle on the end of the nozzle base body, the sheath liquid inlet and the sample nozzle are all arranged at the same end of the laminar liquid flow channel, the flat nozzle makes the sample liquid appear flat when it is sprayed into the laminar liquid flow channel, and the flat nozzle is located at the center of the laminar liquid flow channel, the width of the laminar liquid flow channel containing the flat nozzle is greater than the width of the other end of the laminar liquid flow channel, so that the middle area of ​​the laminar liquid flow channel forms a tapered section to guide the flow direction of the sheath liquid, and the tapered section is directly opposite to the flat nozzle so that the sample liquid can be sprayed out and flowed down from the two side walls of the tapered section. The flat nozzle is wrapped with sheath liquid, and the long axis of the flat nozzle and the laminar liquid flow channel are parallel to each other so that the sample liquid flowing out of the flat nozzle can directly flow into the space between the two side walls of the tapered section. In the direction along which the sample flows downward, the position of the liquid outlet of the sheath liquid nozzle is higher than the position of the liquid outlet of the flat nozzle. Under the drive of the injection mechanism, the flow rate of the sheath liquid ranges from 128uL / s to 146uL / s and the flow rate ratio between the sheath liquid and the sample liquid is between 31.4 and 35.8 to stratify the sample. After the sample is stratified, the imaging module images the sample to obtain a clear image.

2. The shaped component imaging system according to claim 1, characterized in that: The frame includes a syringe mounting frame and four syringe plates arranged on the syringe mounting frame.

3. The shaped component imaging system according to claim 2, characterized in that: The syringe mounting frame is in an "L" shape as a whole, and its short side portion is the solenoid valve mounting portion.

4. The shaped component imaging system according to claim 3, characterized in that: Each of the injector plates includes an injector plate body, a sliding hole arranged on one end of the injector plate body away from the solenoid valve mounting portion, and a bottom plate groove arranged on the end of the injector plate body close to the sliding hole.

5. The shaped component imaging system according to claim 3, characterized in that: The injection mechanism includes a solenoid valve unit arranged on the solenoid valve mounting portion, a syringe unit arranged on the syringe plate, and four motor assemblies arranged on the end surface of the syringe plate facing away from the syringe unit. The solenoid valve unit includes a first solenoid valve, a second solenoid valve, a third solenoid valve, a fourth solenoid valve, a fifth solenoid valve, a sixth solenoid valve, a seventh solenoid valve, and an eighth solenoid valve arranged in sequence from left to right.

6. The shaped component imaging system according to claim 5, characterized in that: The syringe unit includes a cleaning pump assembly, a sheath pump assembly, a sample flow pump assembly, a sample suction pump assembly, and a sample suction needle connected to the sample suction pump assembly and the first solenoid valve, which are arranged on the frame from right to left.

7. The shaped component imaging system according to claim 6, characterized in that: The cleaning pump assembly, the sheath fluid pump assembly, the sample flow pump assembly, and the sample suction pump assembly all have the same structure, and are all composed of a syringe body and a push rod arranged on the end of the syringe body away from the solenoid valve.

8. The shaped component imaging system according to claim 5, characterized in that: Each of the motor assemblies includes a motor mounting plate on the frame, a base plate arranged at one end of the frame, a linear motor fixedly arranged on the motor mounting plate, at least two guide shafts whose ends are respectively arranged on the linear motor and the base plate, and a slider with one end connected to the movable end of the linear motor and movably arranged on the guide shaft.

9. The shaped component imaging system according to claim 1, characterized in that: An exhaust port is also provided at one end of the laminar flow channel close to the sheath liquid inlet.

10. The shaped component imaging system according to claim 9, characterized in that: A breathable and waterproof membrane is arranged at the exhaust port.

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