Sample pad treating fluid for cancer prognosis test strip

Abstract

The invention discloses a sample pad treating fluid of a cancer prognosis test strip. The sample pad treating fluid is prepared from the following raw materials in parts by mass: 5 to 6 parts of sodium carbonate solution, 15 to 25 parts of surfactant, 20 to 40 parts of glycerol, 15 to 18 parts of glucose, 20 to 27 parts of polyacrylamide, 1 to 2 parts of soybean protein and 900 to 950 parts of HEPES. The invention also discloses a preparation method and application of the sample pad treating fluid. The sample pad treating fluid provided by the invention is superior to a sample pad treating fluid in the prior art, and the detection specificity, sensitivity and detection range are improved. The sample pad treating fluid is suitable for preparing the cancer prognosis test strip, and the prepared treating fluid is further used as the sample pad treating fluid for colorectal cancer prognosis detection based on HSPB1, G3BP1 and HAMP ferroptosis genes.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a sample pad treatment liquid formula of a cancer prognosis test strip and its application in colorectal cancer prognosis. Background Art

[0002] Cancer prognosis assessment is crucial to improving the survival rate of cancer patients. With the increasing demand for home testing, postoperative follow-up, and monitoring of recurrence risk, the development of portable and rapid tumor prognosis test strips has become a trend. Among them, the sample pad treatment fluid, as an important component of the test strip detection, not only affects the sensitivity and accuracy of the test strip detection, but also improves the user experience and ease of operation. It is an important component for achieving efficient and reliable detection.

[0003] In order to better meet the prognosis and recurrence monitoring needs of colorectal cancer patients, the present invention discloses a sample pad processing liquid and an application method thereof that can be used for colorectal cancer prognosis detection test strips based on HSPB1, G3BP1 and HAMP ferroptosis genes, thereby improving the specificity and sensitivity of detection. Summary of the invention

[0004] In view of the defect that the existing technical means for processing liquids of sample pads of colorectal cancer prognosis detection test strips are not rich enough, the present invention provides a sample pad processing liquid that can be used for colorectal cancer prognosis detection test strips based on HSPB1, G3BP1 and HAMP ferroptosis genes, thereby improving the specificity and sensitivity of detection and enriching the types of sample pad processing liquids of cancer prognosis detection test strips.

[0005] In addition, the present invention also provides a method for preparing the sample pad treatment liquid of the test strip.

[0006] At the same time, the present invention provides an application method of the sample pad treatment liquid of the test strip.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions A sample pad processing liquid for a cancer prognosis test strip, wherein the raw materials for making the active ingredients thereof, by weight, include: 5-6 parts of sodium carbonate solution, 15-25 parts of surfactant, 20-40 parts of glycerol, 15-18 parts of glucose, 20-27 parts of polyacrylamide, 1-2 parts of soy protein, and 900-950 parts of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonicacid).

[0008] Furthermore, the surfactant is at least one of a Triton X-100 solution or a Tween-20 solution. The concentration of the solute in the surfactant is 2-3 g / L.

[0009] Furthermore, the molecular weight of the polyacrylamide is 10,000-15,000.

[0010] Furthermore, the concentration of HEPES is 10 mM - 25 mM.

[0011] Furthermore, the pH value of the sample pad treatment solution is 6.8-8.2.

[0012] The present invention also provides a method for preparing the sample pad treatment solution, which comprises uniformly mixing sodium carbonate, surfactant, glycerol, glucose, polyacrylamide and soy protein, and then adding HEPES buffer to obtain the test strip sample pad treatment solution of the present invention.

[0013] The present invention also provides an application of the sample pad treatment liquid, which is used as a sample pad treatment liquid for a colorectal cancer prognosis detection test strip based on HSPB1, G3BP1 and HAMP ferroptosis genes.

[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) Filled the gap in sample pad processing fluid for colorectal cancer prognosis detection test strips based on HSPB1, G3BP1 and HAMP ferroptosis genes; (2) The sample pad treatment solution provided by the present invention can effectively reduce nonspecific adsorption, thereby improving the signal-to-noise ratio and enhancing the detection sensitivity of the test strip, so that the three markers for colorectal cancer prognosis detection can be jointly tested on the same test strip, with low detection cost, simple and rapid operation, and intuitive test results; (3) The sample pad treatment solution provided by the present invention contains sodium carbonate in a reasonable proportion, so that the sample pad treatment solution can effectively increase the sensitivity of the test strip when detecting acidic samples and reduce the risk of adverse cross-reactions; (4) The sample pad treatment solution provided by the present invention is easy to store, has good stability, and has a shelf life of up to 24 months at room temperature; (5) The sample pad treatment solution provided by the present invention has a wide range of applications, and can use human serum, plasma, and whole blood as samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a picture of the bottled product of the sample pad treatment solution of the present invention. DETAILED DESCRIPTION

[0016] The above contents of the present invention are further described in detail below by means of specific implementation methods in the form of examples. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. Among them, the instruments and reagents used in the examples can all be commercially available. Figure 1 This is a picture of the bottled product of the sample pad treatment solution of the present invention.

[0017] Example 1 A method for preparing a sample pad treatment solution for a cancer prognosis test strip A method for preparing a sample pad processing liquid for a cancer prognosis test strip, the method comprising the following steps: In the first step, 5.6 g of sodium carbonate, 17.5 g of 2.5 g / L surfactant Triton X-100, 28 g of glycerol, 16 g of glucose, 25.8 g of polyacrylamide, and 1.6 g of soy protein were mixed evenly, and then 930 mL of HEPES was added to make the volume to 1 L to obtain the test strip sample pad treatment solution. The pH value of the test strip sample pad treatment solution was found to be 7.4.

[0018] Example 2-6 A method for preparing a sample pad treatment solution for a cancer prognosis test strip Examples 2-6 are respectively methods for preparing a sample pad treatment solution for a cancer prognosis test strip. The steps are basically the same as those of Example 1, and the only difference is the amount of raw materials and process parameters. See Table 1 below for details:

[0019] Example 7 Performance test of test strip sample pad treatment solution The performance tests were conducted on the sample pad treatment solutions of Examples 1-6, and the main contents are as follows: The first step is to prepare the sample pad treatment solution A in the prior art: 0.01 mol / L PBS solution (pH=7.4) containing 1% BSA, 1.0%-2.0% sucrose, 0.1-0.5% Tween-20, and 0.1%-1.0% PVP polyvinyl pyrrolidone; and sample pad treatment solution B: 3.85 g borax, 10 g 5 g / L amphoteric surfactant S9, 16.5 ml 10 g / L non-surfactant tritonX-100 solution, 20 g PEG6000, 10 g trehalose, 35 g polyvinylpyrrolidone (PVP), 2 g casein, after mixing evenly, add 983.5 ml 0.2 mM Tris buffer to make the volume to 1 L, the pH value of sample pad treatment solution B is 8.5; In the second step, 4.5 mL (150 μL / cm) of the sample pad treatment solution prepared in Examples 1-6, the sample pad treatment solution A in the prior art, and the sample pad treatment solution B in the prior art were taken respectively, and then evenly applied on a 20*300 mm sample pad, placed on a rack to dry overnight, and then used in the colorectal cancer prognosis detection process based on HSPB1, G3BP1 and HAMP ferroptosis genes; In the third step, the reading results of the test strip kits treated with the sample pad treatment solution are shown in Table 2 below: ; From Table 2, we can see that the linear correlation coefficients R of the test strips treated with each sample pad treatment solution are 2 All are greater than 0.99. This indicates that the test results are reliable. In addition, the detection sensitivity and maximum detection range of the test strips treated with each sample pad treatment solution in Examples 1-6 are better than the sample pad treatment solution in the prior art. This shows that the sample pad treatment solution provided by the present invention has higher detection sensitivity and a larger detection range than the treatment solution in the prior art.

[0020] In summary, it can be seen that the sample pad treatment solution provided by the present invention has a high clinical application value for the detection of colorectal cancer prognosis based on HSPB1, G3BP1 and HAMP ferroptosis genes.

[0021] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A sample pad processing liquid for a cancer prognosis test strip, characterized in that: The raw materials for making the active ingredients thereof include, by weight, 5-6 parts of sodium carbonate solution, 15-25 parts of surfactant, 20-40 parts of glycerol, 15-18 parts of glucose, 20-27 parts of polyacrylamide, 1-2 parts of bean protein, and 900-950 parts of HEPES; The surfactant is at least one of a Triton X-100 solution or a Tween-20 solution; The concentration of the solute in the surfactant is 2-3 g / L; The molecular weight of the polyacrylamide is 10000-15000; The concentration of HEPES is 10 mM - 25 mM; The sample pad treatment liquid is used as a sample pad treatment liquid of a test strip for colorectal cancer prognosis detection based on HSPB1, G3BP1 and HAMP ferroptosis genes; The colorectal cancer prognosis detection index is the relative expression of HSPB1, G3BP1 and HAMP ferroptosis genes; The pH value of the treatment solution of the sample pad is 6.8-8.

2.

2. The method for preparing a sample pad processing liquid for a cancer prognosis test strip according to claim 1, characterized in that: The method comprises the following steps: uniformly mixing sodium carbonate, surfactant, glycerol, glucose, polyacrylamide and soy protein, and then adding HEPES buffer to obtain the sample pad treatment solution.

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