Deep sea cold spring macroorganism in-situ capture and fidelity transfer device and method

Abstract

The invention discloses a deep sea cold spring macro organism in-situ capture and fidelity transfer device and method, and relates to the technical field of ocean engineering, the deep sea cold spring macro organism in-situ capture and fidelity transfer device comprises a fixing frame, a plurality of fidelity transfer modules, an in-situ capture module and a pressure regulation and control and macro organism fixing module; each fidelity transfer module is provided with a seawater heat preservation interlayer, low-temperature seawater is injected for temperature buffering, an energy accumulator is arranged, and pressure-maintaining and heat-preservation transfer of the in-situ transfer tank is achieved by pre-filling nitrogen into the energy accumulator and the in-situ transfer tank; the in-situ capturing module conducts lossless capturing on target macroorganisms, the target macroorganisms are put into the in-situ transfer tank, the quick-insertion sealing connector is inserted, the first pressure reduction stop valve is opened, the in-situ transfer tank is pressurized through the energy accumulator, and in-situ capturing and efficient sealing storage of the deep-sea cold spring macroorganisms are achieved. And the pressure regulation and control and macro organism fixing module is used for carrying out pressure regulation and control on the in-situ transfer tank and carrying out character fixing on the target macro organism. The device can realize lossless grabbing of deep sea cold spring macroorganisms, and the macroorganism transfer culture process is full-fidelity.

Description

Technical Field

[0001] The present invention relates to the technical field of marine engineering, and more specifically, to a device and method for in-situ capture and fidelity transfer of deep-sea cold seep macroorganisms. Background Art

[0002] Cold seeps are environments where methane-rich fluids seep rapidly to the seafloor and produce a series of biogeochemical reactions. The carbon-related biogeochemical reactions that occur in this type of environment are mainly organic sulfate reduction, methanogenesis, methane anoxic oxidation, and authigenic carbonate precipitation. These biogeochemical processes have a significant impact on global carbon sequestration, nutrient cycling, climate change, and life evolution. Due to the influence of geological structure, hydrodynamics, complex topography, and special physical and chemical environment, the deep-sea chemoenvironmental ecological environment has a completely different community composition from the shallow sea in the illumination zone, and breeds unique biological species, including a large number of different species of macroorganisms such as mussels, snails, sea cucumbers, tubeworms, and armored shrimps, making it an important place to study the origin of life and adaptive evolution. Cold seeps in different regions have great environmental heterogeneity due to factors such as ocean depth, methane seepage flux, and cold seep development history. Therefore, studying the environmental adaptation mechanism of cold seep macroorganisms and the life response characteristics of different types of macroorganisms under the influence of environmental changes is of great significance to revealing the biological mechanism of extreme environments in deep-sea cold seeps and exploring the origin of deep-sea life.

[0003] At present, the research and development of deep-sea cold seep biological sampling tools are mainly focused on deep-sea trawls, grabs, trap-type large biological samplers, pump-type biological samplers, etc. This type of sampling method cannot take into account the accurate sampling of individual deep-sea macroorganisms without destroying the habitat conditions of the sampling site. At the same time, the current capture of deep-sea macroorganisms is mainly focused on passive heat preservation or pressure maintenance operations, and it is impossible to achieve the active fidelity function during the biological transfer process. The existing deep-sea cold seep macroorganism capture technology is subject to factors such as the extreme environment of the cold seep and the limited space size for obtaining samples. It is often carried out through ROV manipulators (31) to capture single macroorganisms, by directly crushing the organisms and then fixing them with RNAlater to obtain the real-time fixed shape of the macroorganisms or to carry out heat preservation or pressure maintenance after the single macroorganisms are captured. Although the method of crushing the organisms by manipulators and then transporting them can obtain information such as biological traits in a timely manner, it is impossible to conduct relevant research on the response of organisms to environmental changes. At the same time, in order to study the changes in macroscopic and microscopic characteristics of deep-sea cold seep macroorganisms such as physiology, metabolism, and protein under the background of changes in different environmental parameters, it is necessary to maintain the normal physiological and metabolic conditions of deep-sea macroorganisms as much as possible during the collection and capture process. Due to the species specificity of deep-sea cold seep macroorganisms, changes in their environmental parameters during the capture process can easily affect the normal physiological and metabolic activities of macroorganisms, resulting in the inability to conduct normal research on the adaptability of organisms to environmental changes and other macroorganism physiological and metabolic changes. Summary of the invention

[0004] One of the purposes of the present invention is to provide a device for in situ capture and fidelity transfer of deep-sea cold seep macro-organisms, so as to overcome the defects of the above-mentioned prior art that it is impossible to ensure the lossless capture of individuals when capturing deep-sea cold seep macro-organisms and it is impossible to ensure full fidelity when transferring macro-organisms; the second purpose is to provide a method for in situ capture and fidelity transfer of deep-sea cold seep macro-organisms.

[0005] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0006] The present invention provides a deep-sea cold seep macro-organism in-situ capture and fidelity transfer device, comprising a fixing frame, a plurality of fidelity transfer modules, an in-situ capture module and a pressure regulation and macro-organism fixation module;

[0007] Several fidelity transfer modules are evenly arranged in the fixing frame, and the structures of the several fidelity transfer modules are the same, including a seawater insulation interlayer, an in-situ transfer tank, an accumulator, a first pressure reduction stop valve, an air charging valve, a fixing agent injection valve, a second pressure reduction stop valve, a first quick-connect female connector, a second quick-connect female connector and a quick-plug sealing connector;

[0008] The top of the seawater insulation interlayer is provided with an exhaust port and a seawater communication port, and the in-situ transfer tank and the accumulator are arranged inside the seawater insulation interlayer; one end of the inflation valve is suspended in the air, and the other end of the inflation valve is connected to the air inlet end of the accumulator, and the air outlet end of the accumulator is connected to one end of the first pressure reduction stop valve, and the other end of the first pressure reduction stop valve is connected to the air inlet of the in-situ transfer tank;

[0009] The drain port of the in-situ transfer tank is connected to one end of the second pressure-reducing stop valve, and the other end of the second pressure-reducing stop valve is connected to the first quick-connect female connector; the liquid injection end of the in-situ transfer tank is connected to one end of the fixative injection valve, and the other end of the fixative injection valve is connected to the second quick-connect female connector; the quick-plug sealing joint is plugged into the liquid inlet end of the in-situ transfer tank;

[0010] The pressure regulating end of the pressure regulating and macrobiological fixation module is plugged into the first quick-connect female connector, and the liquid outlet end of the pressure regulating and macrobiological fixation module is plugged into the second quick-connect female connector;

[0011] The in-situ capture module is connected to the fixing frame.

[0012] Preferably, the pressure regulation and macro-biological fixation module comprises a control unit, a displacement and drainage PID valve, a high-pressure quantitative injection unit, a biological fixative container, a first quick-connect male connector and a second quick-connect male connector;

[0013] The first control end of the control unit is connected to the control end of the replacement drainage PID valve, the output end of the replacement drainage PID valve is connected to the first quick-connect male connector, and the first quick-connect male connector is plugged into the first quick-connect female connector;

[0014] The second control end of the control unit is connected to the control end of the high-pressure quantitative injection unit, the input end of the high-pressure quantitative injection unit is connected to the liquid outlet of the biological fixative container, the output end of the high-pressure quantitative injection unit is connected to the second quick-connect male connector, and the second quick-connect male connector is plugged into the second quick-connect female connector.

[0015] It can be understood that the pressure regulation and macro-biological fixation module can realize slow changes in system pressure through control of the control unit. Finally, through the biological fixation technology, the biological properties of the macro-organisms at any time in the study of the influence of the pressure change rate on the macro-biological response can be fixed in real time to realize the study of macro-biological responses under the influence of temperature / pressure change rate.

[0016] Preferably, the in-situ capture module comprises an ROV manipulator and a protective cover; the surface of the ROV manipulator is covered with the protective cover; the ROV manipulator covered with the protective cover is connected to the fixing frame by grabbing the fixing frame.

[0017] It is understandable that the ROV manipulator is covered with a protective cover, which effectively prevents the metal clamp from causing damage to the macro-organism's shell and other tissues during the process of grasping the macro-organism, thereby achieving non-destructive grasping of deep-sea cold spring macro-organisms.

[0018] Preferably, each of the fidelity transfer modules further comprises a pressure sensor and a temperature sensor; the pressure sensor and the temperature sensor are arranged on the surface of the in-situ transfer tank.

[0019] Preferably, each of the fidelity transfer modules further comprises a hand-operated valve;

[0020] One end of the hand-operated valve is connected to the other end of the charging valve, and the other end of the hand-operated valve is connected to the air inlet end of the accumulator.

[0021] It is understandable that the hand valve can accurately control the initial pressure when the accumulator is pre-charged with nitrogen.

[0022] Preferably, the device further comprises a temperature regulating module;

[0023] The temperature adjustment module includes a thermostat and a constant temperature water tank;

[0024] The fidelity transfer module is arranged in a constant temperature water tank, and the temperature controller is connected to a control end of the constant temperature water tank.

[0025] It can be understood that the temperature regulation module can realize the slow change of the system temperature. Finally, through the biological fixation technology, the biological properties of the macroorganisms at any time in the study of the influence of the pressure change rate on the macroorganism response can be fixed in real time to realize the study of the macroorganism response under the influence of the temperature / pressure change rate.

[0026] Preferably, RNAlater fixative is provided in the biological fixative container.

[0027] Preferably, the protective cover is made of bionic soft matter material.

[0028] The present invention also discloses a method for in-situ capture and fidelity transfer of deep-sea cold seep macroorganisms, based on the above-mentioned device, characterized in that it includes:

[0029] S1: According to the number of target macroorganisms, a corresponding number of fidelity transfer modules are set in the fixed frame;

[0030] S2: According to the pressure environment of the target area, pre-charge nitrogen into the corresponding accumulator through the charging valve until all the in-situ transfer tanks and accumulators reach the set initial pressure;

[0031] S3: according to the temperature environment of the target area, the exhaust port and the seawater connecting port of the seawater insulation interlayer are opened, and low-temperature seawater that meets the temperature environment of the target area is injected from the seawater connecting port until all the seawater insulation interlayers are filled with low-temperature seawater, and the exhaust port and the seawater connecting port are closed;

[0032] S4: After the ROV manipulator covered with a protective cover grabs the fixed frame and transports it to the target area, it pulls out the quick-plug sealing connector of any fidelity transfer module, and the seawater in the target area enters the corresponding in-situ transfer tank;

[0033] S5: The ROV manipulator covered with a protective cover grabs and collects a target macro-organism, puts it into a corresponding in-situ transfer tank, and inserts a corresponding quick-plug sealing connector to complete the in-situ capture of the target macro-organism;

[0034] S6: Open the corresponding first pressure-reducing stop valve, and use the accumulator to pressurize the in-situ transfer tank until the preset pressure is reached, thereby completing the in-situ pressure maintenance of the target macro-organism;

[0035] S7: repeat steps S4-S6 to complete the in-situ capture and in-situ pressure holding of all target macroorganisms;

[0036] S8: The ROV manipulator wrapped in the protective cover grabs the fixture and transports it to the laboratory environment;

[0037] S9: Place any fidelity transfer module in a constant temperature water tank; connect the corresponding first quick-connect female connector and the first quick-connect male connector, and the corresponding second quick-connect female connector and the second quick-connect male connector;

[0038] S10: The control unit regulates the displacement drainage PID valve, opens the second pressure reduction stop valve, discharges the seawater in the target area in the in-situ transfer tank, and realizes the pressure regulation of the in-situ transfer tank; uses the thermostat to adjust the temperature of the constant temperature water tank, and realizes the temperature regulation of the in-situ transfer tank;

[0039] S11: After the preset time, the control unit regulates the high-pressure quantitative injection unit, opens the fixative injection valve, and injects RNAlater fixative into the in-situ transfer tank to complete the fixation of the properties of the target macroorganism in the fidelity transfer module;

[0040] S12: Repeat steps S9-S11 to complete the fixation of the traits of the target macroorganisms in all fidelity transfer modules.

[0041] The present invention also provides a computer device, including a memory and a processor, wherein the memory stores a computer program, and when the processor executes the computer program, the steps of the above-mentioned method for in situ capture and fidelity transfer of deep-sea cold seep macroorganisms are implemented.

[0042] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0043] The present invention evenly arranges a plurality of fidelity transfer modules in a fixed frame to meet the capture and collection work of multiple target macro-organisms in the same sampling area, thereby improving the efficiency of deep-sea operations; each fidelity transfer module is provided with a seawater insulation interlayer, and low-temperature seawater that meets the temperature environment of the target area is injected from the seawater connecting port as a temperature buffer before entering the water, thereby ensuring the heat preservation transfer of the in-situ transfer tank; an accumulator is provided, and nitrogen is pre-charged into the accumulator and the in-situ transfer tank through an air charging valve before entering the water, thereby ensuring the pressure-maintaining transfer of the in-situ transfer tank; a quick-plug sealing joint is provided at the liquid inlet end of the in-situ transfer tank, thereby achieving efficient sealing; the in-situ capture module grabs the fixed frame, transports the fidelity transfer module to the target area, pulls out the quick-plug sealing joint, and the seawater in the target area enters the in-situ transfer tank; the in-situ capture module non-destructively captures the target macro-organism, puts it into the in-situ transfer tank, inserts the corresponding quick-plug sealing joint, and then opens the first pressure-reducing stop valve, and uses the accumulator to pressurize the in-situ transfer tank until the preset pressure is reached, thereby achieving the in-situ capture and preservation of deep-sea cold spring macro-organisms. Afterwards, the in-situ capture module grabs the fixing frame and transports it to the laboratory environment, and connects it to the pressure control and macro-organism fixing module through the first quick-connect female head and the second quick-connect female head. The pressure control and macro-organism fixing module controls the pressure of the in-situ transfer tank through the pressure regulating end to achieve the response study of the target macro-organism to pressure changes; after a preset time, the target macro-organism in the in-situ transfer tank is fixed through the liquid outlet. The present invention can achieve non-destructive capture of deep-sea cold spring macro-organisms, and the macro-organism transfer and cultivation process is fully fidelity, ensuring that the physiological characteristics of the macro-organism will not change during the overall process of transferring from the deep-sea environment to the laboratory. Through the pressure control and macro-organism fixing module, the characteristics of the macro-organism at any time in the macro-organism response study are fixed in real time, realizing the fixation and analysis of the macro-organism biological information. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1This is a schematic diagram of the structure of a deep-sea cold seep macroorganism in-situ capture and fidelity transfer device described in Example 1;

[0045] Figure 2 This is a schematic diagram of the structure of a deep-sea cold seep macroorganism in-situ capture and fidelity transfer device described in Example 2;

[0046] Figure 3 This is a schematic diagram of the structure of the temperature adjustment module described in Example 2;

[0047] Figure 4 This is a flow chart of a method for in-situ capture and fidelity transfer of deep-sea cold seep macroorganisms as described in Example 3;

[0048] Figure 5 This is a schematic diagram of the structure of a computer device described in Example 3;

[0049] In the figure, 1-fixed frame, 2-fidelity transfer module, 3-in-situ capture module, 4-pressure regulation and macrobiological fixation module, 5-temperature regulation module, 21-seawater insulation interlayer, 22-in-situ transfer tank, 23-accumulator, 24-first pressure reduction stop valve, 25-inflating valve, 26-fixant injection valve, 27-second pressure reduction stop valve, 28-first quick-connect female head, 29-second quick-connect female head, 210-quick-plug sealing joint, 211-pressure sensor, 212-temperature sensor, 213-hand valve, 31-ROV manipulator, 32-protective cover, 41-control unit, 42-displacement drainage PID valve, 43-high-pressure quantitative injection unit, 44-biological fixative container, 45-first quick-connect male head, 46-second quick-connect male head, 51-thermostat, 52-constant temperature water tank. DETAILED DESCRIPTION

[0050] The drawings are for illustrative purposes only and should not be construed as limiting the present patent;

[0051] In order to better illustrate the present embodiment, some parts in the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product;

[0052] It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0053] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0054] Example 1

[0055] This embodiment provides a deep-sea cold spring macro-organism in-situ capture and fidelity transfer device, such as Figure 1 As shown, it includes a fixing frame 1, a plurality of fidelity transfer modules 2, an in-situ capture module 3 and a pressure regulation and macrobiological fixation module 4;

[0056] Several fidelity transfer modules 2 are evenly arranged in the fixing frame 1, and the structures of the several fidelity transfer modules 2 are the same, including a seawater insulation interlayer 21, an in-situ transfer tank 22, an accumulator 23, a first pressure reduction stop valve 24, an air charging valve 25, a fixing agent injection valve 26, a second pressure reduction stop valve 27, a first quick-connect female connector 28, a second quick-connect female connector 29 and a quick-connect sealing joint 210;

[0057] The top of the seawater insulation interlayer 21 is provided with an exhaust port and a seawater communication port, and the in-situ transfer tank 22 and the accumulator 23 are arranged inside the seawater insulation interlayer 21; one end of the inflation valve 25 is suspended, and the other end of the inflation valve 25 is connected to the air inlet end of the accumulator 23, and the air outlet end of the accumulator 23 is connected to one end of the first pressure reduction stop valve 24, and the other end of the first pressure reduction stop valve 24 is connected to the air inlet of the in-situ transfer tank 22;

[0058] The drain port of the in-situ transfer tank 22 is connected to one end of the second pressure reducing stop valve 27, and the other end of the second pressure reducing stop valve 27 is connected to the first quick-connect female connector 28; the liquid injection end of the in-situ transfer tank 22 is connected to one end of the fixing agent injection valve 26, and the other end of the fixing agent injection valve 26 is connected to the second quick-connect female connector 29; the quick-plug sealing joint 210 is plugged into the liquid inlet end of the in-situ transfer tank 22;

[0059] The pressure regulating end of the pressure regulating and macrobiological fixation module 4 is plugged into the first quick-connect female connector 28 , and the liquid outlet end of the pressure regulating and macrobiological fixation module 4 is plugged into the second quick-connect female connector 29 ;

[0060] The in-situ capture module 3 is connected to the fixing frame 1 .

[0061] In the specific implementation process, the present embodiment evenly arranges a number of fidelity transfer modules 2 in the fixed frame 1 to meet the capture and collection work of multiple target macroorganisms in the same sampling area, thereby improving the efficiency of deep-sea operations; each fidelity transfer module 2 is provided with a seawater insulation interlayer 21, and low-temperature seawater that meets the temperature environment of the target area is injected from the seawater connecting port as a temperature buffer before entering the water, thereby ensuring the heat preservation transfer of the in-situ transfer tank 22; an accumulator 23 is provided, and nitrogen is pre-charged into the accumulator 23 and the in-situ transfer tank 22 through the charging valve 25 before entering the water, thereby ensuring the pressure-maintaining transfer of the in-situ transfer tank 22; A quick-plug sealing joint 210 is provided at the liquid inlet end of the transfer tank 22 to achieve efficient sealing; the in-situ capture module 3 grabs the fixing frame 1, transports the fidelity transfer module 2 to the target area, pulls out the quick-plug sealing joint 210, and the seawater in the target area enters the in-situ transfer tank 22; the in-situ capture module 3 non-destructively captures the target macro-organism, puts it into the in-situ transfer tank 22, inserts the corresponding quick-plug sealing joint 210, and then opens the first pressure-reducing stop valve 24, and uses the accumulator 23 to pressurize the in-situ transfer tank 22 until the preset pressure is reached, thereby realizing the in-situ capture and preservation of deep-sea cold spring macro-organisms. Afterwards, the in-situ capture module 3 grabs the fixing frame 1 and transports it to the laboratory environment, and connects it to the pressure control and macro-organism fixing module 4 through the first quick-connect female connector 28 and the second quick-connect female connector 29. The pressure control and macro-organism fixing module 4 controls the pressure of the in-situ transfer tank 22 through the pressure regulating end to achieve the response study of the target macro-organism to pressure changes; after a preset time, the target macro-organism in the in-situ transfer tank 22 is fixed through the liquid outlet. This embodiment can achieve non-destructive capture of deep-sea cold spring macro-organisms, and the macro-organism transfer and cultivation process is fully fidelity, ensuring that the physiological characteristics of the macro-organism will not change during the overall process of transferring from the deep-sea environment to the laboratory. Through the pressure control and macro-organism fixing module 4, the characteristics of the macro-organism at any time in the macro-organism response study are fixed in real time, realizing the fixation and analysis of the macro-organism biological information.

[0062] Example 2

[0063] This embodiment provides a deep-sea cold spring macro-organism in-situ capture and fidelity transfer device, including a fixing frame 1, a plurality of fidelity transfer modules 2, an in-situ capture module 3, a pressure control and macro-organism fixation module 4 and a temperature adjustment module 5;

[0064] like Figure 2 As shown, several of the fidelity transfer modules 2 are evenly arranged in the fixing frame 1, and the structures of the several fidelity transfer modules 2 are the same, including a seawater insulation interlayer 21, an in-situ transfer tank 22, an accumulator 23, a first pressure reduction stop valve 24, an air charging valve 25, a fixing agent injection valve 26, a second pressure reduction stop valve 27, a first quick-connect female connector 28, a second quick-connect female connector 29, a quick-plug sealing joint 210, a pressure sensor 211, a temperature sensor 212 and a hand valve 213;

[0065] An exhaust port and a seawater connecting port are provided on the top of the seawater insulation interlayer 21, and the in-situ transfer tank 22 and the accumulator 23 are arranged inside the seawater insulation interlayer 21; one end of the inflation valve 25 is suspended, and the other end of the inflation valve 25 is connected to one end of the hand-operated valve 213, and the other end of the hand-operated valve 213 is connected to the air inlet end of the accumulator 23, and the air outlet end of the accumulator 23 is connected to one end of the first pressure-reducing stop valve 24, and the other end of the first pressure-reducing stop valve 24 is connected to the air inlet of the in-situ transfer tank 22; the pressure sensor 211 and the temperature sensor 212 are arranged on the surface of the in-situ transfer tank 22;

[0066] The drain port of the in-situ transfer tank 22 is connected to one end of the second pressure reducing stop valve 27, and the other end of the second pressure reducing stop valve 27 is connected to the first quick-connect female connector 28; the liquid injection end of the in-situ transfer tank 22 is connected to one end of the fixing agent injection valve 26, and the other end of the fixing agent injection valve 26 is connected to the second quick-connect female connector 29; the quick-plug sealing joint 210 is plugged into the liquid inlet end of the in-situ transfer tank 22;

[0067] In this embodiment, the biological fixative container 44 is filled with RNAlater fixative.

[0068] The pressure regulation and macrobiological fixation module 4 includes a control unit 41, a displacement and drainage PID valve 42, a high-pressure quantitative injection unit 43, a biological fixative container 44, a first quick-connect male connector 45 and a second quick-connect male connector 46;

[0069] The first control end of the control unit 41 is connected to the control end of the replacement drainage PID valve 42, the output end of the replacement drainage PID valve 42 is connected to the first quick-connect male connector 45, and the first quick-connect male connector 45 is plugged into the first quick-connect female connector 28;

[0070] The second control end of the control unit 41 is connected to the control end of the high-pressure quantitative injection unit 43, the input end of the high-pressure quantitative injection unit 43 is connected to the liquid outlet of the biological fixative container 44, the output end of the high-pressure quantitative injection unit 43 is connected to the second quick-connect male connector 46, and the second quick-connect male connector 46 is plugged into the second quick-connect female connector 29;

[0071] The temperature adjustment module 5 includes a thermostat 51 and a constant temperature water tank 52;

[0072] The fidelity transfer module 2 is arranged in a constant temperature water tank 52, and the temperature controller 51 is connected to the control end of the constant temperature water tank 52;

[0073] The in-situ capture module 3 includes an ROV manipulator 31 and a protective cover 32; the surface of the ROV manipulator 31 is covered with the protective cover 32; the ROV manipulator 31 covered with the protective cover 32 is connected to the fixing frame 1 by grabbing the fixing frame 1;

[0074] In this embodiment, the material of the protective cover 32 is a bionic soft material.

[0075] It can be understood that the in-situ capture of deep-sea cold spring macro-organisms in this embodiment is mainly based on the function of the ROV manipulator 31 and the cooperation of the in-situ transfer tank 22. The ROV manipulator 31 is structurally covered with a protective cover 32 made of bionic soft material. This structural design effectively avoids the damage to the shell and other tissues caused by the metal clamp in the process of grasping the organism, and realizes the non-destructive capture of deep-sea cold spring macro-organisms. When the macro-organism is captured, the ROV manipulator 31 opens the in-situ transfer tank 22, and the tank will be filled with seawater in the habitat of the macro-organism. Then the ROV manipulator 31 grabs the target macro-organism of the required sample and puts it into the in-situ transfer tank 22. The opening of the in-situ transfer tank 22 is designed with a quick-insert sealing joint 210. After the target macro-organism is placed in the tank, the ROV manipulator 31 grabs the quick-insert sealing joint 210 and inserts it into the tank mouth position to complete the in-situ capture of the single-sample target macro-organism.

[0076] The fidelity transfer module 2 mainly relies on the seawater insulation interlayer 21, the in-situ transfer tank 22 and the accumulator 23. The in-situ transfer tank 22 and the accumulator 23 are all wrapped in the seawater insulation interlayer 21. The insulation function is achieved by first opening the exhaust port in the seawater insulation interlayer 21 according to the temperature environment of the target site to be investigated before the device enters the sea, and filling the seawater insulation interlayer 21 with low-temperature seawater that meets the temperature environment of the target sampling site through the seawater connecting port, as a temperature increase buffer during the insulation and pressure transfer process. The exhaust port is opened throughout the water injection process. After the water injection is completed, the gas in the seawater insulation interlayer 21 is also fully discharged. At this time, the exhaust port is closed and the seawater insulation interlayer 21 is completely sealed. The pressure-maintaining function is achieved by connecting the accumulator 23 to an external gas storage tank before the device enters the sea. According to the pressure environment of the target site to be investigated, the in-situ transfer tank 22 and the accumulator 23 are pre-charged with nitrogen for initial pressure. The initial pressure of the accumulator 23 can be further precisely regulated by the hand-operated valve 213 on the accumulator 23. The pressurization in the pressure-maintaining transfer tank can balance the internal and external pressures under high-pressure conditions, satisfying the convenience of opening the transfer tank. After the in-situ non-destructive capture of deep-sea cold spring macro-organisms is completed, the in-situ transfer tank 22 is in a sealed state, which is filled with seawater from the in-situ environment of the target sampling site and the required deep-sea cold spring target macro-organisms. At this time, the first pressure-reducing stop valve 24 is opened, and the in-situ transfer tank 22 is continuously pressurized according to the initial pressure to achieve the pressure-maintaining function.

[0077] When there are multi-sequence work tasks of collecting multiple species / biological individuals in the same sampling area, a number of fidelity transfer modules 2 are evenly arranged on the fixed frame 1, and different initial pressures are pre-charged for multiple accumulators 23. The ROV manipulator 31 grabs the fixed frame 1 and transports it to the sampling area, and the above process is repeated for each fidelity transfer module 2.

[0078] The pressure control and macro-biological fixation module 4 is not immersed in water. After the fidelity transfer module 2 is transported back to the laboratory environment, the pressure control and macro-biological fixation module 4 is connected to the in-situ transfer tank 22 through the first quick-connect male connector 45 and the second quick-connect male connector 46, respectively completing the tasks of displacement drainage and biological fixative injection. The pressure control function is realized by regulating the displacement drainage PID valve 42 through the control unit 41, and realizing the pressure reduction operation by discharging the seawater in the in-situ transfer tank 22; in addition, the pressure can be slowly reduced through the first pressure reduction stop valve 24 of the in-situ transfer tank 22 and the accumulator 23, so as to realize the response study of macro-biology to the slow pressure reduction process. The temperature control function is realized by the action of the seawater insulation interlayer 21, so that the temperature of the seawater in the in-situ transfer tank 22 remains roughly unchanged, such as Figure 3 As shown, it is then placed in a constant temperature water tank 52 in the laboratory, and the temperature of the water in the constant temperature water tank 52 is set to achieve temperature control. After the target macroorganism is maintained at the target pressure or temperature for a period of time, the biological metabolism tends to be stable, and the control unit 41 uses the high-pressure quantitative injection unit 43 to inject RNAlater fixative from the biological fixative container 44 into the in-situ transfer tank 22 through the fixative injection valve 26. During this process, the replacement drainage PID valve 42 drains the in-situ transfer tank 22 through the drain port, so that the fluid in the in-situ transfer tank 22 is replaced isothermally and isobarically, and finally the properties of the target macroorganism are fixed, so as to realize the target macroorganism response research under the influence of variable temperature / variable pressure rate.

[0079] Example 3

[0080] This embodiment provides a method for in situ capture and fidelity transfer of deep-sea cold seep macroorganisms, based on the device described in Example 1 or 2, such as Figure 4 As shown, including:

[0081] S1: According to the number of target macroorganisms, a corresponding number of fidelity transfer modules are set in the fixed frame;

[0082] S2: According to the pressure environment of the target area, pre-charge nitrogen into the corresponding accumulator through the charging valve until all the in-situ transfer tanks and accumulators reach the set initial pressure;

[0083] S3: according to the temperature environment of the target area, the exhaust port and the seawater connecting port of the seawater insulation interlayer are opened, and low-temperature seawater that meets the temperature environment of the target area is injected from the seawater connecting port until all the seawater insulation interlayers are filled with low-temperature seawater, and the exhaust port and the seawater connecting port are closed;

[0084] S4: After the ROV manipulator covered with a protective cover grabs the fixed frame and transports it to the target area, it pulls out the quick-plug sealing connector of any fidelity transfer module, and the seawater in the target area enters the corresponding in-situ transfer tank;

[0085] S5: The ROV manipulator covered with a protective cover grabs and collects a target macro-organism, puts it into a corresponding in-situ transfer tank, and inserts a corresponding quick-plug sealing connector to complete the in-situ capture of the target macro-organism;

[0086] S6: Open the corresponding first pressure-reducing stop valve, and use the accumulator to pressurize the in-situ transfer tank until the preset pressure is reached, thereby completing the in-situ pressure maintenance of the target macro-organism;

[0087] S7: repeat steps S4-S6 to complete the in-situ capture and in-situ pressure holding of all target macroorganisms;

[0088] S8: The ROV manipulator wrapped in the protective cover grabs the fixture and transports it to the laboratory environment;

[0089] S9: Place any fidelity transfer module in a constant temperature water tank; connect the corresponding first quick-connect female connector and the first quick-connect male connector, and the corresponding second quick-connect female connector and the second quick-connect male connector;

[0090] S10: The control unit regulates the displacement drainage PID valve, opens the second pressure reduction stop valve, discharges the seawater in the target area in the in-situ transfer tank, and realizes the pressure regulation of the in-situ transfer tank; uses the thermostat to adjust the temperature of the constant temperature water tank, and realizes the temperature regulation of the in-situ transfer tank;

[0091] S11: After a preset time, the control unit regulates the high-pressure quantitative injection unit, opens the fixative injection valve, and injects RNAlater fixative into the in-situ transfer tank to complete the fixation of the properties of the target macroorganism in the fidelity transfer module;

[0092] S12: Repeat steps S9-S11 to complete the fixation of the traits of the target macroorganisms in all fidelity transfer modules.

[0093] This embodiment also provides a computer device, such as Figure 5 As shown, at least one processor 01 , at least one communication interface 02 , at least one memory 03 and at least one communication bus 04 .

[0094] In the embodiment of the present application, the number of the processor 01 , the communication interface 02 , the memory 03 , and the communication bus 04 is at least one, and the processor 01 , the communication interface 02 , and the memory 03 communicate with each other through the communication bus 04 .

[0095] Processor 01 may include one or more processing cores, such as a 4-core processor, an 8-core processor, etc. Processor 01 may be implemented in at least one hardware form of a digital signal processor (DSP), a field-programmable gate array (FPGA), and a programmable logic array (PLA). Processor 01 may also include a main processor and a coprocessor. The main processor is a processor for processing data in an awake state, also known as a central processing unit (CPU); the coprocessor is a low-power processor for processing data in a standby state. In some embodiments, processor 01 may be integrated with a graphics processing unit (GPU), which is responsible for rendering and drawing the content to be displayed on the display screen. In some embodiments, processor 01 may also include an artificial intelligence (AI) processor, which is used to process computing operations related to machine learning. Equipped with a high-performance multi-core processor and integrated GPU, it provides powerful computing power, supports real-time image processing and complex data analysis, ensures high efficiency and accuracy in deep-sea macro-organism detection tasks, integrates AI coprocessors, effectively accelerates the execution of related macro-organism target detection algorithms, and improves the accuracy and speed of macro-organism recognition and classification.

[0096] The memory 03 may include one or more computer-readable storage media, which may be non-transitory. The memory 03 may also include high-speed random access memory and non-volatile memory, such as one or more disk storage devices and flash memory storage devices. The high-speed and stable communication interface designed in the memory 03 supports wired and wireless data transmission, ensuring that the data collected by deep-sea detectors, underwater cameras and other equipment can be transmitted to the processor 01 in real time and accurately for processing. At the same time, the memory 03 supports multiple communication protocols to ensure compatibility with deep-sea detection equipment of different manufacturers and models.

[0097] Among them, the memory 03 stores a program, and the processor 01 can call the program stored in the memory 03, and the program is used to execute the steps of the deep-sea cold seep macro-organism in situ capture and fidelity transfer method described in Example 3.

[0098] The same or similar reference numerals correspond to the same or similar components;

[0099] The terms used in the drawings to describe positional relationships are only used for illustrative purposes and should not be construed as limiting this patent;

[0100] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A device for in-situ capture and fidelity transfer of deep-sea cold seep macroorganisms, characterized in that: It comprises a fixing frame (1), a plurality of fidelity transfer modules (2), an in-situ capture module (3) and a pressure regulation and macrobiological fixing module (4); A plurality of the fidelity transfer modules (2) are evenly arranged in the fixing frame (1), and the plurality of the fidelity transfer modules (2) have the same structure, and all include a seawater insulation interlayer (21), an in-situ transfer tank (22), an accumulator (23), a first pressure reduction stop valve (24), an air charging valve (25), a fixing agent injection valve (26), a second pressure reduction stop valve (27), a first quick-connect female connector (28), a second quick-connect female connector (29), and a quick-connect sealing joint (210); The top of the seawater heat-insulating interlayer (21) is provided with an exhaust port and a seawater communication port, and the in-situ transfer tank (22) and the accumulator (23) are arranged inside the seawater heat-insulating interlayer (21); one end of the inflation valve (25) is suspended in the air, and the other end of the inflation valve (25) is connected to the air inlet end of the accumulator (23); the air outlet end of the accumulator (23) is connected to one end of the first pressure-reducing stop valve (24), and the other end of the first pressure-reducing stop valve (24) is connected to the air inlet of the in-situ transfer tank (22); The drainage port of the in-situ transfer tank (22) is connected to one end of the second pressure reducing stop valve (27), and the other end of the second pressure reducing stop valve (27) is connected to the first quick-connect female connector (28); the liquid injection end of the in-situ transfer tank (22) is connected to one end of the fixing agent injection valve (26), and the other end of the fixing agent injection valve (26) is connected to the second quick-connect female connector (29); the quick-plug sealing joint (210) is plugged into the liquid inlet end of the in-situ transfer tank (22); The pressure regulating end of the pressure regulating and macro-biological fixation module (4) is plugged into the first quick-connect female connector (28), and the liquid outlet end of the pressure regulating and macro-biological fixation module (4) is plugged into the second quick-connect female connector (29); The in-situ capture module (3) is connected to the fixing frame (1).

2. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 1, characterized in that: The pressure regulation and macro-biological fixation module (4) comprises a control unit (41), a displacement drainage PID valve (42), a high-pressure quantitative injection unit (43), a biological fixative container (44), a first quick-connect male connector (45) and a second quick-connect male connector (46); The first control end of the control unit (41) is connected to the control end of the replacement drainage PID valve (42), the output end of the replacement drainage PID valve (42) is connected to the first quick-connect male connector (45), and the first quick-connect male connector (45) is plugged into the first quick-connect female connector (28); The second control end of the control unit (41) is connected to the control end of the high-pressure quantitative injection unit (43), the input end of the high-pressure quantitative injection unit (43) is connected to the liquid outlet of the biological fixative container (44), the output end of the high-pressure quantitative injection unit (43) is connected to the second quick-connect male connector (46), and the second quick-connect male connector (46) is plugged into the second quick-connect female connector (29).

3. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 1, characterized in that: The in-situ capture module (3) comprises an ROV manipulator (31) and a protective cover (32); the surface of the ROV manipulator (31) is covered with the protective cover (32); the ROV manipulator (31) covered with the protective cover (32) is connected to the fixing frame (1) by grabbing the fixing frame (1).

4. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 1, characterized in that: Each of the fidelity transfer modules (2) further comprises a pressure sensor (211) and a temperature sensor (212); the pressure sensor (211) and the temperature sensor (212) are arranged on the surface of the in-situ transfer tank (22).

5. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 1, characterized in that: Each of the fidelity transfer modules (2) further comprises a hand-operated valve (213); One end of the hand-operated valve (213) is connected to the other end of the charging valve (25), and the other end of the hand-operated valve (213) is connected to the air inlet end of the accumulator (23).

6. A deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to any one of claims 1 to 5, characterized in that: The device also includes a temperature adjustment module (5); The temperature adjustment module (5) comprises a temperature controller (51) and a constant temperature water tank (52); The fidelity transfer module (2) is arranged in a constant temperature water tank (52), and the temperature controller (51) is connected to a control end of the constant temperature water tank (52).

7. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 2, characterized in that: The biological fixative container (44) is filled with RNAlater fixative.

8. The deep-sea cold seep macroorganism in-situ capture and fidelity transfer device according to claim 3, characterized in that: The material of the protective cover (32) is a bionic soft material.

9. A method for in-situ capture and fidelity transfer of deep-sea cold seep macroorganisms, based on the device according to any one of claims 1 to 8, characterized in that: include: S1: According to the number of target macroorganisms, a corresponding number of fidelity transfer modules are set in the fixed frame; S2: According to the pressure environment of the target area, pre-charge nitrogen into the corresponding accumulator through the charging valve until all the in-situ transfer tanks and accumulators reach the set initial pressure; S3: according to the temperature environment of the target area, the exhaust port and the seawater connecting port of the seawater insulation interlayer are opened, and low-temperature seawater that meets the temperature environment of the target area is injected from the seawater connecting port until all the seawater insulation interlayers are filled with low-temperature seawater, and the exhaust port and the seawater connecting port are closed; S4: After the ROV manipulator covered with a protective cover grabs the fixed frame and transports it to the target area, it pulls out the quick-plug sealing connector of any fidelity transfer module, and the seawater in the target area enters the corresponding in-situ transfer tank; S5: The ROV manipulator covered with a protective cover grabs and collects a target macro-organism, puts it into a corresponding in-situ transfer tank, and inserts a corresponding quick-plug sealing connector to complete the in-situ capture of the target macro-organism; S6: Open the corresponding first pressure-reducing stop valve, and use the accumulator to pressurize the in-situ transfer tank until the preset pressure is reached, thereby completing the in-situ pressure maintenance of the target macro-organism; S7: repeat steps S4-S6 to complete the in-situ capture and in-situ pressure holding of all target macroorganisms; S8: The ROV manipulator wrapped in the protective cover grabs the fixture and transports it to the laboratory environment; S9: Place any fidelity transfer module in a constant temperature water tank; connect the corresponding first quick-connect female connector and the first quick-connect male connector, and the corresponding second quick-connect female connector and the second quick-connect male connector; S10: The control unit regulates the displacement drainage PID valve, opens the second pressure reduction stop valve, discharges the seawater in the target area in the in-situ transfer tank, and realizes the pressure regulation of the in-situ transfer tank; uses the thermostat to adjust the temperature of the constant temperature water tank, and realizes the temperature regulation of the in-situ transfer tank; S11: After a preset time, the control unit regulates the high-pressure quantitative injection unit, opens the fixative injection valve, and injects RNAlater fixative into the in-situ transfer tank to complete the fixation of the properties of the target macroorganism in the fidelity transfer module; S12: Repeat steps S9-S11 to complete the fixation of the traits of the target macroorganisms in all fidelity transfer modules.

10. A computer device comprising a memory and a processor, wherein the memory stores a computer program, characterized in that: When the processor executes the computer program, the steps of the method for in situ capture and fidelity transfer of deep-sea cold seep macroorganisms described in claim 9 are implemented.

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