A composition comprising trichoderma and mortierella and its application in the biological control of panax notoginseng

Abstract

The present application relates to the technical field of plant disease prevention and control, and particularly relates to a composition containing Trichoderma and Mortierella, a preparation and application thereof. The composition contains strains, conidia and / or volatile substances of Trichoderma afroharzianum 3NG-1 and Mortierella alpina B28-1, and the volume ratio of the Trichoderma afroharzianum 3NG-1 to the Mortierella alpina B28-1 is 20:1-1:20. The composition and the preparation thereof are used for controlling the disease and biological agents of Panax notoginseng, relieving the continuous cropping effect of Panax notoginseng in the growth process and promoting the growth of Panax notoginseng. The present application has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant disease prevention and control, and particularly relates to a composition containing Trichoderma and Mortierella and application thereof in biological prevention and control of Panax notoginseng. BACKGROUND

[0002] Panax notoginseng is a traditional Chinese medicinal material with the effects of activating blood circulation to dissipate blood stasis and relieving swelling and pain, and has significant effects on cardiovascular and cerebrovascular diseases, and has high medicinal and economic values. The demand for Panax notoginseng is increasing day by day. In order to meet the market demand, Panax notoginseng is planted in large areas year after year. Due to the unique growth habit of Panax notoginseng, pathogenic bacteria are easily accumulated, and diseases are easily caused, among which, soil-borne root rot disease is particularly serious, causing Panax notoginseng to wither and die, and the continuous cropping obstacle is aggravated.

[0003] The imbalance of soil microbial community structure, the decrease of the abundance of beneficial microorganisms and the accumulation of soil-borne pathogens are the main reasons for the occurrence of Panax notoginseng root rot disease. At present, the methods for preventing and controlling Panax notoginseng root rot disease include crop rotation, chemical pesticide control and soil sterilization, but all have certain drawbacks. For example, crop rotation requires a long period, which is difficult to maintain the sustainable development of Panax notoginseng industry; chemical pesticide control may cause excessive residues and heavy metal problems; soil sterilization requires a high cost and kills the indigenous beneficial microorganisms in the soil. Biological control is an environmentally friendly method for preventing and controlling plant diseases by using beneficial microorganisms to regulate the composition of soil microorganisms. Many beneficial microorganisms have been used as biological fertilizers or microbial agents in agricultural production.

[0004] As an important biocontrol fungus, Trichoderma has the advantages of broad-spectrum and high efficiency, and has a certain control effect on many kinds of plant diseases, such as Fusarium oxysporum, Rhizoctonia solani, Pythium ultimum, Pseudomonas syringae and Xanthomonas campestris. Trichoderma has multiple biological control mechanisms, including competition, hyperparasitism, induced resistance and antibiosis, etc. Trichoderma can not only inhibit the growth of pathogenic bacteria, but also promote plant growth, improve plant stress resistance, root nutrient utilization rate and regulate soil ecological balance. However, the effect of using single Trichoderma to control crop diseases is sometimes not ideal, so the synergistic effect of Trichoderma and other biological or non-biological factors in disease prevention is more and more concerned.

[0005] Mortierella is a dominant genus widely existing in the rhizosphere microbial community of some healthy plants. It can produce auxin (IAA), iron carrier and increase the content of soil available nitrogen and available phosphorus to promote plant growth. In addition, it is reported that Mortierella can regulate soil microbial community to significantly alleviate the root rot disease of Panax ginseng caused by Fusarium oxysporum. The arachidonic acid produced by Mortierella can activate the plant defense pathway as a signal substance to reduce the occurrence of diseases. Therefore, Mortierella has great potential in improving plant nutrient absorption efficiency, protecting crops from adverse conditions and resisting pathogenic bacteria.

[0006] Over the past decade, multi-omics sequencing technologies have been widely applied to plant microbiome research, revealing that complex rhizosphere microbial communities, rather than single microbial strains, protect plants from pathogen infection. Studies have shown that mixtures of non-antagonistic biocontrol agents can overcome the shortcomings of single agents applied in the field, such as limited efficacy, limited broad-spectrum antimicrobial activity, and inability to maximize their effectiveness. To address these issues, multiple, distantly related, and non-antagonistic strains are combined and applied to the field. While each strain exerts its own biocontrol effects, the strains work together to coordinate and control plant diseases, effectively enhancing control capabilities and significantly improving soil ecological structure. Previous studies have shown that both Trichoderma and Mortierella can be used as biocontrol strains to mitigate the occurrence of Panax notoginseng root rot and alleviate continuous cropping problems. However, whether their combined application can synergistically improve the stress resistance of Panax notoginseng plants and alleviate these problems remains unclear. Summary of the Invention

[0007] In order to solve the above problems, the present invention provides a composition comprising Trichoderma and Mortierella and its application in biological control of Panax notoginseng.

[0008] In order to achieve the above objectives, the technical solutions adopted by the present invention are as follows:

[0009] Specifically, the first aspect of the present invention provides a composition for biological control of Panax notoginseng, comprising strains, conidia and / or volatiles of bacterial cultures of Trichoderma afroharzianum 3NG-1 and Mortierella alpina B28-1, wherein the Trichoderma afroharzianum 3NG-1 has been disclosed in Lifen Luo, et al, “Negative Plant-Soil Feedback Drivenby Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng”, Frontiers in Microbiology, Volume 10, Article 1597, July 2019, fig 7, and the Mortierella alpina B28-1 has been disclosed in the applicant's patent (CN116218683B), and the deposit number of Mortierella alpina B28-1 is CGMCC No.40006; classification name: Mortierella alpina; the preservation unit is the General Microbiology Center of China Culture Collection Administration; the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101; the preservation date is December 10, 2021.

[0010] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 20:1 to 1:20.

[0011] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 10:1 to 1:10.

[0012] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 5:1 to 1:5.

[0013] As an optional manner, in the above composition, the volume ratio of the Trichoderma africanum 3NG-1 to the Mortierella alpina B28-1 is 4:1 to 1:1.

[0014] The second aspect of the present invention provides a preparation comprising the composition described in the first aspect.

[0015] As an optional mode, in the above preparation, the preparation is a mixed bacterial suspension.

[0016] As an optional method, in the above preparation, the concentration of the spore suspension of Trichoderma africanum 3NG-1 and Mortierella alpina B28-1 is 1.0×10 6 ~1.0×10 8 CFU / mL.

[0017] As an optional method, in the above preparation, the concentration of the spore suspension of Trichoderma africanum 3NG-1 and the spore suspension of Mortierella alpina B28-1 is 1.0×10 7 CFU / mL.

[0018] The third aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for biological control of Panax notoginseng diseases.

[0019] As an optional mode, in the above application, the Panax notoginseng disease is root rot.

[0020] As an optional manner, in the above application, the Panax notoginseng disease is black spot disease.

[0021] The fourth aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for alleviating the continuous cropping effect in the soil during the growth of Panax notoginseng.

[0022] The fifth aspect of the present invention provides the use of the composition described in the first aspect or the preparation described in the second aspect for promoting the growth of Panax notoginseng.

[0023] The sixth aspect of the present invention provides use of the composition described in the first aspect or the preparation described in the second aspect for preparing a biological control preparation.

[0024] The seventh aspect of the present invention provides use of the composition described in the first aspect or the preparation described in the second aspect for preparing a plant growth-promoting preparation.

[0025] As an optional manner, in the application described in the sixth or seventh aspect above, the preparation is used for Panax notoginseng.

[0026] The beneficial effects of the present invention are as follows:

[0027] The present invention provides a method for treating Panax notoginseng root rot by irrigating 50 mL of a mixed spore suspension of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 at a volume ratio of v(Mertierella alpina B28-1):v(Trichoderma africanum 3NG-1)=1. The method is used to treat Panax notoginseng root rot by irrigating 50 mL of a mixed spore suspension of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 at a volume ratio of v(Mertierella alpina B28-1):v(Trichoderma africanum 3NG-1)=1. After 48 hours of root irrigation, the leaves are inoculated with the Panax notoginseng black spot pathogen Alternaria panax. The results show that the lesion area of ​​the Panax notoginseng leaves is significantly reduced. The spore suspension concentration of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 was 1.0×10 6 and 1.0×10 7 CFU / mL, and the roots of Panax notoginseng were irrigated with spore suspensions mixed at different volume ratios. Compared with the sterilized water control, the mixed spore suspensions increased the survival rate of Panax notoginseng and reduced the incidence of root rot. Among them, the volume ratio of v (Mortellini alpina B28-1): v (Trichoderma harzianum 3NG-1) was 4:1, and the concentrations of both bacteria were 1.0×10 7 CFU / mL, and mixed at v(alpine Mortierella B28-1):v(African Harzianum 3NG-1)=1:4, both could significantly promote the survival rate of Panax notoginseng and reduce the incidence of Panax notoginseng root rot. The other two bacteria concentrations were 1.0×10 7 CFU / mL, and when mixed at a ratio of v(alpine Mortierella B28-1):v(African Harzianum 3NG-1)=1:1, the incidence of root rot of Panax notoginseng can be significantly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The confrontation between Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on PDA medium. Figure a shows the Mortierella alpina B28-1 control, b shows the Trichoderma africanum 3NG-1 control, and c shows the confrontation between the two strains.

[0029] Figure 2 Figure 3 shows the fresh weight growth of mycelium of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 after shake-culturing on different concentrations of crude Panax notoginseng saponins as the sole carbon source. Different lowercase letters in the figure indicate significant differences at the P < 0.05 level as tested by Duncan's new multiple range test.

[0030] Figure 3 The plates show Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 cultured against the main Panax notoginseng root rot pathogens Z5, F3, M, and RS6 for 4-6 days. Figure a shows a control plate of four pathogens cultured against Mortierella alpina B28-1, b shows a plate of four pathogens cultured against Mortierella alpina B28-1, c shows a control plate of four pathogens cultured against Trichoderma africanum 3NG-1, and d shows a plate of four pathogens cultured against Trichoderma africanum 3NG-1.

[0031] Figure 4 The combined use of Mortierella alpina B28-1 and Trichoderma harzianum 3NG-1 enhances the resistance of Panax notoginseng plants to black spot disease. Different lowercase letters in the figure indicate significant differences at the P < 0.05 level as tested by Duncan's new multiple range test.

[0032] Figure 5 The effect of the combined use of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on the survival rate and disease incidence of Panax notoginseng. Different lowercase letters in the figure indicate significant differences at the P < 0.05 level as tested by Duncan's new multiple range test. DETAILED DESCRIPTION

[0033] The present invention will be further described below with reference to specific embodiments. It should be understood that the specific embodiments described herein are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0034] If no specific techniques or conditions are specified in the examples, the experiments were carried out according to the techniques or conditions described in the literature in the field or according to the product instructions. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0035] The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are commercially available products unless otherwise specified.

[0036] Example 1 Evaluation of the antagonistic effect between Mortierella alpina B28-1 and Trichoderma africanum 3NG-1

[0037] Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 were prepared using a sterile cork borer. These strains were then inoculated onto PDA plates using the stand-off plate method, approximately 5.5 cm apart. Four replicates were used for each treatment. A control was inoculated on one side with Mortierella alpina B28-1 or Trichoderma africanum 3NG-1 and a blank PDA agar block on the other side. The plates were incubated at 28°C. The colony radius of each strain was measured when the colonies grew to a point where they were adjacent to each other.

[0038] The results are shown in Table 1 and Figure 1 As shown, compared with the control, Mortierella alpina B28-1 significantly promoted the growth of Trichoderma africanum 3NG-1; there was no significant difference in the colony radius of Mortierella alpina B28-1, indicating that there was no mutual antagonism between the two strains.

[0039] Table 1 Antagonistic effects of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on the main pathogens of Panax notoginseng root rot

[0040]

[0041] Note: * indicates significant difference compared with the control at P<0.05 level after t-test.

[0042] Example 2 Utilization Ability of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on Crude Panax notoginseng Saponins

[0043] Mortierella alpina B28-1 strain and Trichoderma africanum 3NG-1 were placed in different concentrations (0.01%, 0.1% and 1%) of crude saponin liquid culture medium and shake culture medium composition was: 3g NaNO3, 1g KH2PO4, 0.5g KCl, 0.5g MgSO4·7H2O, 0.01g FeSO4· 7H2O , 0.1g (or 1g, 10g) crude saponin, 15g agar, 1000mL distilled water, dissolved, mixed, and adjusted to pH 7.0 with 1mol / LNaOH solution. The culture medium without crude saponin was used as the control. Alpine Mortierella B28-1 and African Trichoderma harzianum 3NG-1 were shaken at room temperature and 120rpm for 7d and 5d respectively, after which the water was drained and the agar block was removed to weigh the fresh weight of the mycelium. The results are as follows Figure 2As shown, compared with the control, Mortierella alpina B28-1 was able to grow using low concentrations of crude saponins, while high concentrations of crude saponins inhibited its growth; Trichoderma africanum 3NG-1 was able to grow using notoginseng crude saponins at all three tested concentrations, especially at high concentrations.

[0044] Example 3 Inhibitory Effects of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on the Main Pathogens of Panax notoginseng Root Rot

[0045] Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 were co-cultured with the main pathogens of Panax notoginseng root rot using opposing plates. The four Panax notoginseng pathogen strains tested included Cylindrocarpondestructans RS6, Fusarium solani F3, Fusarium oxysporum Z5, and Monographella cucumerina M. A sterile microporator was used to prepare bacterial cakes from Mortierella alpina B28-1, Trichoderma africanum 3NG-1, and the tested Panax notoginseng pathogens. The two strains were then inoculated onto polydimethylsiloxane (PDA) agar plates, each with the four pathogens, approximately 5.5 cm apart. Five replicates were used for each treatment. The control consisted of a blank PDA agar block inoculated with the pathogen on one side and a blank PDA agar block on the other. The plates were incubated at 28°C. The colony radius of the pathogens was measured after 4-6 days, and the inhibition rate was calculated.

[0046] Inhibition rate (%) = (control colony radius - confrontation culture colony radius) / control colony radius × 100

[0047] The results are shown in Table 2 and Figure 3 As shown in the results, both the above-mentioned Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 can significantly inhibit the growth of the main pathogens of Panax notoginseng root rot. Among them, Mortierella alpina B28-1 has a better inhibitory effect on Fusarium solani, with an inhibition rate of 29.9%, which is more than three times that of other pathogens. The inhibition rate of Trichoderma africanum 3NG-1 on the four pathogens reached more than 70%.

[0048] Table 2 Antagonistic effects of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 on the main pathogens of Panax notoginseng root rot

[0049]

[0050] Example 4 Application of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 in Combining to Induce Panax notoginseng Plants to Enhance Resistance to Black Spot Disease

[0051] A mixed spore suspension of Mortierella alpina B28-1 strain and Trichoderma africanum 3NG-1 is prepared, and the preparation method comprises the following steps:

[0052] 1) inoculating Mortierella alpina B28-1 strain and Trichoderma africanum 3NG-1 on PDA medium to obtain Mortierella alpina B28-1 strain colonies and Trichoderma africanum 3NG-1 strain colonies respectively;

[0053] 2) Add 20 mL of sterile water to each of the two culture dishes of the strains in step 1), scrape the conidia on the surface, and filter through four layers of sterile gauze to obtain conidia suspensions of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1, respectively. The concentration of both suspensions was adjusted to 1 × 10 7 CFU / mL;

[0054] 3) The two conidia suspensions in step 2) were thoroughly mixed at a volume ratio of v (Mortierella alpina B28-1):v (Trichoderma africanum 3NG-1) = 1:4.

[0055] The roots of Panax notoginseng were irrigated with 50 mL of a mixed conidia suspension of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 (7MT14), and treated with sterile water (CK) and single strain treatment (7M10: 10 mL of conidia suspension of Mortierella alpina B28-1 mixed with 40 mL of sterile water, 7T40: 40 mL of conidia suspension of Trichoderma africanum 3NG-1 mixed with 10 mL of sterile water). After 48 hours of treatment, the leaves were inoculated with Alternaria panax, the black spot pathogen of Panax notoginseng, and the lesion area was measured after the onset of the disease.

[0056] The control effect on the Panax notoginseng black spot pathogen Alternaria panax was calculated according to the formula: control effect (%) = (relative lesion area of ​​control - relative lesion area of ​​treatment) / relative lesion area of ​​control × 100%.

[0057] The synergistic ratio was calculated according to the Abbott method.

[0058] Efficiency ratio = Cobs / %Cexp = Cobs / [A+B-(AB / 100)]

[0059] Where: Cobs represents the actual control efficacy of the mixed bacterial suspension; %Cexp represents the expected control efficacy of the mixed bacterial suspension; A and B represent the actual control efficacy of the two single bacterial agents against the pathogens, respectively. If the synergistic ratio is greater than 1, the mixed bacterial suspension exhibits synergistic effects.

[0060] The results are as follows Figure 3As shown in Table 3, compared with the sterilized water control and the single strain conidia suspension, the lesion area of ​​the mixed conidia suspension treatment was significantly reduced, and the synergistic ratio of the mixed conidia suspension treatment was 8.64, indicating that the combination of Mortierella alpina B28-1 strain and African Trichoderma harzianum 3NG-1 synergistically enhanced the resistance of Panax notoginseng leaves to black spot disease.

[0061] Table 3 Synergistic ratio of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 in controlling black spot disease on Panax notoginseng leaves

[0062]

[0063] Note: * indicates synergistic effect

[0064] Example 5 Application of Mortierella alpina B28-1 strain and Trichoderma harzianum strain 3NG-1 in alleviating the obstacle of continuous cropping of Panax notoginseng

[0065] A mixed spore suspension of Mortierella alpina B28-1 strain and Trichoderma africanum 3NG-1 is prepared, and the preparation method comprises the following steps:

[0066] 1) inoculating Mortierella alpina B28-1 strain and Trichoderma africanum 3NG-1 on PDA medium to obtain Mortierella alpina B28-1 strain colonies and Trichoderma africanum 3NG-1 strain colonies respectively;

[0067] 2) Add 20 mL of sterile water to each of the two culture dishes of the strains in step 1), scrape the conidia on the surface, and filter through four layers of sterile gauze to obtain conidia suspensions of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1, respectively. The concentration of both suspensions was adjusted to 1 × 10 6 and 1×10 7 CFU / mL;

[0068] 3) The two conidia suspensions of the same concentration in step 2) were thoroughly mixed at different volume ratios as follows:

[0069] 6MT41: 1.0×10 6 The concentration of the spore suspension of 6M40 (6M40: the conidia suspension of the same concentration of Mortierella alpina B28-1) was mixed at a ratio of v(Mertierella alpina B28-1) to v(sterile water) at a ratio of 4:1; the conidia suspension of the same concentration of Trichoderma harzianum 3NG-1 was mixed at a ratio of v(Mertierella alpina B28-1) to v(sterile water) at a ratio of 1:4.

[0070] 7MT41: 1.0 x 10 7 CFU / mL of spore suspension concentration, mixed in the ratio of v(G. monticola B28-1):v(T. africanum 3NG-1) = 4:1, with single strain treatment as control (7M40: G. monticola B28-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(G. monticola B28-1):v(sterilized water) = 4:1; 7T10: T. africanum 3NG-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(T. africanum 3NG-1):v(sterilized water) = 1:4);

[0071] 7MT11: 1.0 x 10 7 CFU / mL of spore suspension concentration, mixed in the ratio of v(G. monticola B28-1):v(T. africanum 3NG-1) = 1:1, with single strain treatment as control (7M25: G. monticola B28-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(G. monticola B28-1):v(sterilized water) = 1:1; 7T25: T. africanum 3NG-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(T. africanum 3NG-1):v(sterilized water) = 1:1);

[0072] 7MT14: 1.0 x 10 7 CFU / mL of spore suspension concentration, mixed in the ratio of v(G. monticola B28-1):v(T. africanum 3NG-1) = 1:4, with single strain treatment as control (7M10: G. monticola B28-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(G. monticola B28-1):v(sterilized water) = 1:4; 7T40: T. africanum 3NG-1 strain conidial spore suspension of the same concentration mixed in the ratio of v(T. africanum 3NG-1):v(sterilized water) = 4:1);

[0073] The mixed conidial spore suspension of 50 mL of G. monticola B28-1 and T. africanum 3NG-1 was used for root irrigation treatment of continuous cropping G. elatum, and equal volume of sterilized water and single strain conidial spore suspension was used as control, 5 replicates for each treatment, once every 15 days, a total of three times of root irrigation, and the survival rate and root rot incidence were counted two months after the first root irrigation treatment.

[0074] The joint synergistic ratio of the mixed conidial spore suspension of G. monticola B28-1 and T. africanum 3NG-1 on G. elatum root rot was calculated.

[0075] The control effect on Panax notoginseng root rot was calculated according to the formula: control effect (%) = (control incidence rate - treatment incidence rate) / control incidence rate × 100%.

[0076] The synergistic ratio was calculated according to the Abbott method.

[0077] Efficiency ratio = Cobs / %Cexp = Cobs / [A+B-(AB / 100)]

[0078] Where: Cobs represents the actual control efficacy of the mixed bacterial suspension; %Cexp represents the expected control efficacy of the mixed bacterial suspension; A and B represent the actual control efficacy of the two single bacterial agents against the pathogens, respectively. If the synergistic ratio is greater than 1, the mixed bacterial suspension exhibits synergistic effects.

[0079] The results are as follows Figure 4 As shown in Table 4, compared with the sterile water control, the mixed conidia suspension of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 significantly reduced the incidence of root rot in Panax notoginseng and increased its seedling survival rate. When the spore suspension of the two strains was mixed at a ratio of v (M. alpina B28-1): v (sterile water) = 4:1 and applied to the roots of Panax notoginseng, the seedling survival rate of Panax notoginseng was significantly higher than that of the single strain treatment. In terms of root rot incidence, this mixed spore suspension also showed a lower incidence rate. In addition, the synergistic ratios of the 6MT41, 7MT41, and 7MT14 treatments were all greater than 1, indicating that the combination of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 synergized to reduce the incidence of root rot in Panax notoginseng. In summary, the combined use of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 has a better synergistic effect than the single strains, further alleviating the obstacles to continuous cropping of Panax notoginseng.

[0080] Table 4 Synergistic ratio of Mortierella alpina B28-1 and Trichoderma africanum 3NG-1 in the control of Panax notoginseng root rot

[0081]

[0082] Note: * indicates synergistic effect

[0083] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A composition for biological control of Panax notoginseng, characterized in that: The composition comprises Trichoderma africanum ( Trichoderma afroharzianum ) Conidia of 3NG-1 and Mortierella alpina ( Mortierella alpina ) conidia of B28-1, and the volume ratio of the conidia of Trichoderma harzianum 3NG-1 to the conidia of Mortierella alpina B28-1 is 1:4 or 4:

1.

2. A preparation comprising the composition of claim 1.

3. The preparation according to claim 2, characterized in that: The preparation is a mixed bacterial suspension.

4. The preparation according to claim 3, wherein: The concentration of the spore suspension of Trichoderma africanum 3NG-1 and Mortierella alpina B28-1 is 1.0×10 6 ~1.0×10 8 CFU / mL.

5. Use of the composition according to claim 1 or the preparation according to any one of claims 2 to 4 for biological control of Panax notoginseng diseases.

6. The use according to claim 5, characterized in that: The Panax notoginseng disease is root rot or black spot.

7. Use of the composition of claim 1 or the preparation of any one of claims 2 to 4 for alleviating the soil continuous cropping effect during the growth of Panax notoginseng.

8. Use of the composition according to claim 1 or the preparation according to any one of claims 2 to 4 for promoting the growth of Panax notoginseng.

9. Use of the composition according to claim 1 or the preparation according to any one of claims 2 to 4 in preparing a biological control preparation or a plant growth promotion preparation.

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