Automatic preparation device for microbial culture medium

Abstract

The present invention relates to the technical field related to culture medium preparation, and discloses an automatic preparation device for microbial culture medium. In order to solve the problem of component contamination caused by leakage of culture medium into the external environment during preparation, a medicine delivery cartridge containing various components is arranged on a medicine dispensing ring frame. When culture medium needs to be prepared, the medicine delivery cartridge is inserted into a filling plug, and the injection method is used to replace the dropper for feeding, thereby avoiding external environmental contamination and achieving precise feeding by using the scale on the medicine delivery cartridge. After the injection of various components of the culture medium is completed, a central disk is driven by a motor to rotate, thereby realizing the self-rotation of the bevel gear on the central disk along the fixed gear ring, thereby causing the culture medium test tube fixed on the bevel gear to rotate continuously, thereby realizing mixing between the various components in the culture medium test tube, and finally achieving the effect of sterile environment preparation and test tube rotation and stirring.

Description

Technical Field

[0001] The present invention relates to the technical field related to culture medium preparation, and in particular to an automatic preparation device for microbial culture medium. Background Art

[0002] Automated microbial culture medium preparation is designed to facilitate operator preparation, mixing, and dispensing of culture media, thereby improving experimental efficiency and culture medium quality. Automated microbial culture medium preparation has a wide range of applications in fields such as microbiology research, medical diagnostics, environmental monitoring, the food industry, drug development, and genetic engineering.

[0003] At present, the preparation of microbial culture medium still mainly relies on manual operation, for example, using a dropper to inject the culture medium into a rubber tube. However, this method is difficult to ensure the accurate preparation of each sample, the error is large, and it cannot meet the needs of high-precision experiments. In addition, during the preparation process, in order to ensure the full mixing of different culture medium components, it is often necessary to use a stirring rod to stir to ensure the uniformity of the various components of the culture medium. However, in actual applications, we have found that the culture medium usually needs to be kept sterile to prevent contamination by external environmental factors. Both the dropper injection and the stirring rod stirring methods will expose the culture medium to the external environment, thereby increasing its risk of contamination. Once the culture medium is contaminated, it will not only lead to component loss, but may also cause component denaturation, seriously affecting the accuracy of the experimental results. Summary of the Invention

[0004] The present invention proposes an automatic microbial culture medium preparation device, which has the advantages of sterile environment preparation and test tube rotation stirring, and is used to solve the problem of component contamination caused by leakage of culture medium into the external environment during the preparation process mentioned in the background art.

[0005] To achieve the above-mentioned purpose, the present invention adopts the following technical scheme: an automatic preparation device for microbial culture medium, comprising: a mounting base, a center disk driven by a motor is provided on the inner top; a bevel gear, a rotating shaft is movably mounted on the outer side of the center disk, and a fixed gear ring meshing with the bevel gear is fixedly mounted on the top of the mounting base; a culture medium test tube is fastened to the rotating shaft of the bevel gear through a test tube fixing frame, a test tube cap is inserted into the top of the culture medium test tube, and a filling plug is fixedly mounted on the top of the test tube cap; a medicine dispensing ring frame, movably mounted on the top of the center disk, and a medicine feeding barrel is arranged on the surface of the medicine dispensing ring frame, and a return push spring is provided between the medicine feeding barrel and the medicine dispensing ring frame.

[0006] Furthermore, there are multiple drug delivery cartridges, which are arranged in a circular shape at equal angles on the surface of the drug dispensing ring frame, and each drug delivery cartridge is filled with culture medium of different components.

[0007] Furthermore, a tightening wedge block is fixedly connected to the bottom of the test tube cover, a tightening screw is threadedly connected to the test tube cover, and a tightening ring is movably installed at the bottom of the tightening screw.

[0008] Furthermore, a weight column is placed inside the culture medium test tube.

[0009] Furthermore, guide buffer sliders are fixedly installed at both ends of the counterweight column, and a stirring rod is provided on the outside of the counterweight column.

[0010] Furthermore, an air jet is fastened to the inner side of the test tube cover, an air jet piston is mounted inside the air jet, one end of the piston driving arm is fastened to the top of the air jet piston, and the other end is fastened to a magnetic block; an air jet hole is opened on the side of the piston driving arm, and the air jet hole is communicated with the inner cavity of the air jet through the inner cavity of the piston driving arm and the inner cavity of the air jet.

[0011] Furthermore, a one-way air intake valve is fixed on the top of the air jet tube.

[0012] Furthermore, a detection cylinder is threadedly connected to the top of the test tube cover, and an air supply pipe is fastened to the inside of the test tube cover; a detection piston is movably installed inside the detection cylinder, and a detection spring is connected between the detection piston and the detection cylinder.

[0013] Furthermore, a one-way air supply valve is fixedly installed on the surface of the detection piston.

[0014] The present invention has the following beneficial effects:

[0015] The present invention provides an automatic microbial culture medium preparation device. The core component of the device is a dispensing ring frame, on which are carefully arranged multiple drug delivery cartridges containing different culture medium components. These drug delivery cartridges not only store the required ingredients, but are also designed with the requirements of aseptic operation in mind, avoiding the risk of external environmental contamination associated with traditional dropper feeding.

[0016] When culture medium preparation is needed, the drug delivery cartridge is precisely inserted into the injection plug to deliver the culture medium components into the test tube by injection. This process is not only fast and efficient, but also, with the help of the clearly marked scale on the drug delivery cartridge, it can ensure that each delivery is accurate, thereby greatly improving the accuracy and consistency of culture medium preparation.

[0017] After the culture medium components are successfully injected into the test tube, the motor is activated, driving the central disk to begin rotating. The bevel gear on the central disk then rotates along the fixed gear ring. This design cleverly utilizes the principle of gear transmission, allowing the culture medium tube fixed to the bevel gear to rotate smoothly and continuously. This continuous rotation of the test tube ensures that the culture medium components within the test tube are thoroughly mixed, achieving an efficient and sterile mixing process.

[0018] In summary, this automated microbial culture medium preparation device not only achieves precise batching in a sterile environment but also ensures thorough mixing of all culture medium components through its innovative test tube rotation and stirring method. The application of this device will greatly improve the efficiency and quality of microbiology experiments, providing strong support for scientific research and production. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0020] The present invention can be more clearly understood from the following detailed description with reference to the accompanying drawings, in which:

[0021] Figure 1 This is a schematic diagram of the overall external three-dimensional structure of the present invention;

[0022] Figure 2 It is a three-dimensional schematic diagram of the drug dispensing structure of the present invention;

[0023] Figure 3 This is a schematic diagram of the center disk installation and drive structure;

[0024] Figure 4 This is a three-dimensional schematic diagram of the installation position of the culture medium test tube;

[0025] Figure 5 This is a schematic diagram of the external three-dimensional structure of the culture medium test tube;

[0026] Figure 6 This is a schematic diagram of the internal cross-sectional three-dimensional structure of the culture medium test tube;

[0027] Figure 7 for Figure 6 The enlarged structural diagram at E in the middle;

[0028] Figure 8 for Figure 6 Enlarged structural diagram at F in the middle.

[0029] In the figure: 1. Mounting base; 2. Fixed gear ring; 3. Dispensing ring frame; 4. Drug delivery cartridge; 5. Return push spring; 6. Center disk; 7. Bevel gear; 8. Test tube fixing frame; 9. Culture medium test tube; 10. Motor; 11. Test tube cover; 110. Filling plug; 111. Tightening wedge block; 12. Tightening ring; 120. Tightening screw; 13. Detection cylinder; 14. Counterweight column; 140. Guide buffer slider; 141. Stirring rod; 15. Detection piston; 150. Detection spring; 151. One-way air supply valve; 16. Air supply pipe; 17. Jet cylinder; 171. One-way suction valve; 18. Jet piston; 19. Piston drive arm; 190. Jet hole; 20. Magnetic block. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0031] For example 1, please refer to Figure 1 and Figure 3 As can be seen, the mounting base 1 is fixed in the desired position by the flange at the bottom. A motor 10 is fixedly mounted on the top of the mounting base 1. A center disk 6, located at the top of the mounting base 1, is fixedly mounted on the output shaft of the motor 10. The motor 10 can drive the center disk 6 to rotate. A plurality of bevel gears 7 are circumferentially mounted on the outer side of the center disk 6 at equal angles. Correspondingly, a fixed gear ring 2 is fixedly mounted on the top of the mounting base 1, meshing with the bevel gears 7. When the motor 10 causes the bevel gears 7 to rotate circumferentially around the fixed gear ring 2 via the center disk 6, the meshing between the bevel gears 7 and the fixed gear ring 2 forces the bevel gears 7 to rotate on their own.

[0032] The culture medium test tube 9 is fastened to the rotating shaft of the bevel gear 7 through the test tube fixing frame 8. Figure 3 and Figure 4 It can be clearly seen that the test tube fixing frame 8 is composed of two elastic clamping blocks, wherein one clamping block is fixed on the bevel gear 7 and the other is sleeved on the screw. When the culture medium test tube 9 needs to be fixed, the culture medium test tube 9 is placed between the two clamping blocks, and the bolt on the screw is turned to make the two clamping blocks relatively close, and finally the culture medium test tube 9 between the clamping blocks is clamped and tightened.

[0033] Moreover, in order to prevent the material inside the culture medium test tube 9 from being poured out during the rotation process, a test tube cover 11 is inserted into the top of the culture medium test tube 9, thereby sealing the inner cavity of the culture medium test tube 9 and preventing the raw materials inside the culture medium test tube 9 from being contaminated by the external environment. Figure 4 and Figure 5 It can be seen that a filling plug 110 is fixedly installed on the top of the test tube cover 11. The filling plug 110 is generally made of rubber. When the needle on the drug delivery cartridge 4 pierces the filling plug 110, the various components of the culture medium can be injected into the sterile environment. Figure 1 and Figure 2As can be clearly seen, a central disk 6 is topped with a dispensing ring frame 3 connected by a bearing. Multiple drug delivery cartridges 4 are arranged at equal angles around the dispensing ring frame 3. A return spring 5 is positioned between each drug delivery cartridge 4 and the dispensing ring frame 3. Under normal circumstances, the elastic force of the return spring 5 forces the drug delivery cartridge 4 upward, causing the needle at the bottom of the drug delivery cartridge 4 to move relatively away from the culture medium tube 9. When culture medium is to be prepared, the drug delivery cartridge 4 containing the desired component is moved above the corresponding culture medium tube 9. This downward pressure on the drug delivery cartridge 4 and the return spring 5 compresses the return spring 5 until the needle at the bottom of the drug delivery cartridge 4 pierces the injection plug 110, injecting the culture medium component in the drug delivery cartridge 4 into the inner lumen of the culture medium tube 9 (the injection method for other components is similar). During this process, the culture medium is completely isolated from the outside world, significantly preventing contamination of the culture medium components by the external environment. Furthermore, scale lines are provided on the outside of the drug delivery cartridge 4, allowing the operator to accurately determine the injection amount of the culture medium component, ensuring relatively accurate injection of each component.

[0034] Specifically, during the culture medium preparation process, the operator rotates the dispensing ring 3 and moves the desired drug delivery cartridge 4 to the top of the culture medium test tube 9. After pressing the drug delivery cartridge 4 downward, the needle at the bottom of the drug delivery cartridge 4 pierces the injection plug 110 and enters the lumen of the culture medium test tube 9, thereby aseptically injecting the culture medium components in the drug delivery cartridge 4. When the injection is completed, the return spring 5 pushes the drug delivery cartridge 4 away from the culture medium test tube 9, completing the injection of the culture medium component. The injection method for other culture medium components is the same.

[0035] After culture media of different components are input into the culture media test tube 9, the motor 10 is used to drive the center disk 6 to rotate, thereby causing the center disk 6 to drive the bevel gear 7 to rotate circumferentially. During this process, the bevel gear 7 rotates around the fixed gear ring 2, thereby driving the culture media test tube 9 to rotate, so that the various components inside the culture media test tube 9 are evenly mixed, thereby ensuring that the culture media raw materials inside the culture media test tube 9 can be mixed and stirred under sterile conditions.

[0036] Finally, after the culture medium components in the culture medium test tube 9 have been stirred completely, the culture medium test tube 9 can be taken out from the test tube fixing rack 8 .

[0037] On this basis, in order to prevent the test tube cover 11 from accidentally detaching from the end of the culture medium test tube 9, Figure 5-Figure 7It can be clearly seen that the bottom of the test tube cover 11 is fixedly connected to a tightening wedge block 111 located on the outside of the culture medium test tube 9. The tightening wedge block 111 is preferably made of rubber. There are three tightening wedge blocks 111, and the three tightening wedge blocks 111 are distributed at equal angles in a ring shape at the bottom of the test tube cover 11. A tightening screw 120 is threadedly connected to the test tube cover 11. When the tightening screw 120 is turned, it pushes the tightening ring 12 movably installed at the bottom to move downward synchronously. Since the inner ring of the tightening ring 12 is located on the outside of the tightening wedge block 111, when the tightening ring 12 is continuously pushed downward, combined with the outer side of the tightening wedge block 111 being an inclined surface, the squeezing strength of the tightening ring 12 on the outer side of the tightening wedge block 111 will continue to increase, and the tightening wedge block 111 will be tightly attached to the outer side of the culture medium test tube 9. The compression of the tightening ring 12 increases the friction between the tightening wedge block 111 and the culture medium test tube 9 , thereby preventing the test tube cover 11 from accidentally falling off from the end of the culture medium test tube 9 .

[0038] Example 2 is a further improvement on the basis of Example 1. In order to make the culture medium of each component in the culture medium test tube 9 uniformly mixed, please refer to Figure 6 It can be seen that a counterweight column 14 is placed inside the culture medium test tube 9. When the culture medium test tube 9 rotates, the counterweight column 14 moves in the culture medium test tube 9, thereby enhancing the stirring of the various component culture media in the inner cavity of the culture medium test tube 9. In addition, since the counterweight column 14 is made of metal, in order to prevent the counterweight column 14 from being damaged when it hits the culture medium test tube 9, guide buffer sliders 140 are fixedly installed at both ends of the counterweight column 14. The guide buffer sliders 140 enable the counterweight column 14 to move in a direction in the culture medium test tube 9, thereby avoiding direct collision between the counterweight column 14 and the culture medium test tube 9. At the same time, a plurality of stirring rods 141 are provided on the outside of the counterweight column 14. The stirring rods 141 can further enhance the stirring of the various component culture media in the culture medium test tube 9, thereby further enhancing the mixing efficiency of the various component culture media raw materials in the inner cavity of the culture medium test tube 9.

[0039] On this basis, combined with Figure 6-Figure 8 It can be seen that the inner side of the test tube cover 11 has a jet cylinder 17 coaxially connected to the connecting plate. The jet cylinder 17 is in the shape of a hollow cylinder, and a jet piston 18 is mounted inside the jet cylinder. The piston drive arm 19 is fastened to the top of the jet piston 18. Figure 8It can be clearly seen that the piston drive arm 19 is U-shaped. One end of the piston drive arm 19 passes through the jet cylinder 17 and is connected to the jet piston 18. The other end is located outside the jet cylinder 17. In addition, this end is tightly connected to a magnet 20. Under normal conditions, the magnet 20 will attract the counterweight column 14, forcing the two to move relatively close. Furthermore, a plurality of jet holes 190 are opened on the side of the piston drive arm 19, and the jet holes 190 are directly connected through the inner cavity of the piston drive arm 19 and the inner cavity of the jet cylinder 17. Figure 8 As shown, when the jet piston 18 moves upward, the airflow in the inner cavity of the jet cylinder 17 and located at the top of the jet piston 18 will be output from the jet hole 190; similarly, when the jet piston 18 moves downward, the airflow inside the culture medium test tube 9 will be unidirectionally transported from the one-way suction valve 171 fixed on the top of the jet cylinder 17 to the inner cavity of the jet cylinder 17.

[0040] Specific, combined Figure 1 As shown, when the culture medium test tube 9 is in a relatively vertical state, the test tube cover 11 is located above the end of the culture medium test tube 9. Figure 6 and Figure 8 As shown, the counterweight column 14 is located at the bottom of the inner cavity of the culture medium test tube 9 under the action of its own gravity, and the magnetic attraction of the magnetic block 20 to the counterweight column 14 forces the piston drive arm 19 to pull the jet piston 18 to move downward. At this time, the inner cavity of the jet cylinder 17 is located above the jet piston 18 to store a large amount of air.

[0041] As motor 10 drives center disk 6 to rotate, bevel gear 7 rotates culture tube 9 along fixed gear ring 2, causing test tube cap 11 to rotate toward the bottom of tube 9. During this process, as tube 9 rotates 180°, counterweight column 14 also tends to move toward test tube cap 11 due to the rotation of tube 9. As counterweight column 14 moves along the interior of tube 9, it stirs the interior of tube 9 using its externally fixed stirring rod 141, thereby mixing the various components within tube 9. Furthermore, when counterweight column 14 approaches test tube cap 11, it first contacts magnetic block 20, thereby pushing piston drive arm 19. This pulls jet piston 18 toward one-way air intake valve 171, squeezing air from jet cylinder 17 and expelling it through jet orifice 190. At this time, the air jet hole 190 is immersed in the culture medium, and the bubbles bulging out through the air jet hole 190 realize the disturbance of the culture medium in the inner cavity of the culture medium test tube 9, further enhancing the mixing of the various component culture media in the inner cavity of the culture medium test tube 9.

[0042] As the culture medium test tube 9 rotates further, the culture medium test tube 9 will rotate 180° again until the test tube cover 11 moves to the square of the culture medium test tube 9 again. At this time, the counterweight column 14 moves downward under gravity, and the magnetic block 20 pushes the jet piston 18 away from the one-way suction valve 171 through the piston drive arm 19. The air at the top of the culture medium test tube 9 enters the inner cavity of the jet cylinder 17 through the one-way suction valve 171, and waits for the next rotation, and then uses the jet piston 18 to push the airflow in the jet cylinder 17 outward.

[0043] Example 3 is a supplement to Example 2. Please refer to Figure 5-Figure 8 It can be seen that the top of the test tube cover 11 is threadedly connected to the detection cylinder 13, and the inner side of the test tube cover 11 is fastened with an air pipe 16 for communicating the inner cavity of the detection cylinder 13 with the inner cavity of the air jet cylinder 17. Figure 8 It can be seen that when the jet piston 18 moves to the limit away from the one-way air inlet valve 171, the jet cylinder 17 is connected to the inner cavity of the detection cylinder 13 via the air supply pipe 16. A detection piston 15 is movably installed inside the detection cylinder 13, so that the inner cavity of the detection cylinder 13 and the bottom of the detection piston 15 form a relatively sealed area. In addition, a detection spring 150 is connected between the detection piston 15 and the detection cylinder 13. Under the elastic force of the detection spring 150, the detection piston 15 moves downward and approaches the air supply pipe 16. On this basis, combined with Figure 7 It can be seen that a one-way air supply valve 151 is fixedly installed on the surface of the detection piston 15 , and the one-way air supply valve 151 ensures that external air can only be input into the inner cavity of the detection cylinder 13 .

[0044] During specific preparation, the test tube cover 11 is inserted into the top of the culture medium test tube 9 , and the tightening ring 12 is pressed tightly against the tightening wedge block 111 , so that the test tube cover 11 is firmly locked on the top of the culture medium test tube 9 .

[0045] Under normal circumstances, the culture medium test tube 9 is arranged vertically and the test tube cover 11 is at the top of the culture medium test tube 9. At this time, the counterweight column 14 is located at the bottom of the inner cavity of the culture medium test tube 9, and the magnetic block 20 attracts the counterweight column 14, forcing the magnetic block 20 to push the jet piston 18 downward away from the one-way suction valve 171 according to the piston drive arm 19 until the jet piston 18 passes over the air supply pipe 16. At this time, the inner cavity of the jet cylinder 17 is connected to the detection cylinder 13 through the air supply pipe 16.

[0046] Next, the needle at the bottom of the drug delivery barrel 4 pierces the injection plug 110, and the culture medium components within the inner cavity of the drug delivery barrel 4 are injected into the culture medium tube 9. During this process, the injected culture medium causes the inner cavity pressure of the culture medium tube 9 to increase. The air pressure within the inner cavity of the culture medium tube 9 forces the inner cavity pressure of the connected detection barrel 13 to increase simultaneously, thereby pushing the detection piston 15 upward and compressing the detection spring 150. At this point, the detection piston 15 tends to move upward. After all the culture medium components have been injected into the inner cavity of the culture medium tube 9, the internal pressure of the culture medium tube 9 also increases relatively, and the detection piston 15 rises to a certain height. At this point, an outsider can clearly determine whether the interior of the culture medium tube 9 is relatively sealed by checking whether the detection piston 15 rises, thereby determining whether the culture medium in the inner cavity of the culture medium tube 9 is exposed to the external environment and contaminated. Specifically, if the detection piston 15 rises, the inner cavity of the culture medium tube 9 is airtight; otherwise, it indicates that the culture medium tube 9 is leaking.

[0047] As the motor 10 drives the center disk 6 to rotate, the culture medium test tube 9 will rotate. After the culture medium test tube 9 rotates 180°, the counterweight column 14 moves toward the test tube cover 11 and contacts the extrusion piston drive arm 19. The piston drive arm 19 pulls the jet piston 18 across the air pipe 16. At this time, the airflow in the inner cavity of the jet cylinder 17 is squeezed by the jet piston 18 and output from the jet hole 190 to the culture medium in the inner cavity of the culture medium test tube 9. The bubble disturbance is used to further enhance the intensity of the culture medium mixing.

[0048] If there is a leak between the culture medium test tube 9 and the test tube cover 11 during use, at this time, when the culture medium test tube 9 is arranged inverted, the test tube cover 11 is located at the bottom of the culture medium test tube 9. As the jet piston 18 presses the airflow in the inner cavity of the jet cylinder 17 into the inner cavity of the culture medium test tube 9, the airflow enters the inner cavity of the culture medium test tube 9, causing its air pressure to increase. Combined with the fact that the test tube cover 11 is at the bottom at this time, the culture medium will flow out directly along the leaking part, thereby alerting the operator that there is a leakage problem in the culture medium test tube 9 here.

[0049] As the culture medium test tube 9 continues to rotate, it rotates another 180°. At this point, the test tube cover 11 is located at the top of the culture medium test tube 9. The magnet 20, while attracting the counterweight column 14, pulls the jet piston 18 away from the one-way air inlet valve 171 through the piston drive arm 19 until the jet piston 18 passes over the air supply pipe 16. Because the airflow in the inner cavity of the detection cylinder 13 is squeezed by the detection spring 150, when the inner cavity of the detection cylinder 13 and the jet cylinder 17 are connected, the airflow in the jet cylinder 17 will continue to be discharged through the jet hole 190. Combined with the leakage between the culture medium test tube 9 and the test tube cover 11, the airflow in the air supply pipe 16 will continue to be discharged outward until the detection piston 15 moves to the bottom of the detection cylinder 13.

[0050] When the leaking culture medium tube 9 rotates another 180°, the tube cover 11 rotates below the culture medium tube 9, and the jet piston 18 again squeezes the air in the inner cavity of the jet cylinder 17, causing the pressure in the inner cavity of the culture medium tube 9 to increase, further exacerbating the discharge of culture medium from the leaking area of ​​the culture medium tube 9. Furthermore, when the jet piston 18 moves upward, the inner cavity of the jet cylinder 17 at the bottom of the jet piston 18 decreases due to pressure. As a result, the one-way air supply valve 151 draws external air through the air supply pipe 16 and into the jet cylinder 17, thereby reducing the movement resistance of the jet piston 18.

[0051] Finally, after the stirring of the culture medium test tube 9 is completed, if the detection piston 15 has not moved to the bottom of the detection cylinder 13, it means that the inner cavity of the culture medium test tube 9 is still in a relatively sealed state, and the culture medium in the culture medium test tube 9 has not been connected to the outside, and there is no problem of being contaminated by the external environment; on the contrary, if the detection piston 15 reaches the bottom of the detection cylinder 13, it means that the pressure inside the culture medium test tube 9 is consistent with that outside, and there is a leakage, and the culture medium inside the culture medium test tube 9 may be contaminated. In addition, if the leakage site is located between the culture medium test tube 9 and the test tube cover 11, the culture medium material inside the culture medium test tube 9 is discharged, so that the leakage site can be determined, which is convenient for subsequent personnel to quickly find the leakage point for repair.

Claims

1. A microbial culture medium automatic preparation device, characterized in that: include: The mounting base has a central disk driven by a motor on the top inner side; The bevel gear has a rotating shaft movably mounted on the outside of the center disk, and a fixed gear ring meshing with the bevel gear is fixedly mounted on the top of the mounting base; The culture medium test tube is fastened to the rotating shaft of the bevel gear through a test tube fixing frame, the top of the culture medium test tube is plugged with a test tube cover, and the top of the test tube cover is fixedly installed with a filling plug; When the motor causes the bevel gear to rotate circumferentially around the fixed gear ring through the center disk, the meshing between the bevel gear and the fixed gear ring forces the bevel gear to rotate on its own, driving the culture medium test tube to rotate, so that the components inside the culture medium test tube are evenly mixed; The medicine dispensing ring frame is movably mounted on the top of the central disk, and a medicine delivery barrel is arranged on the surface of the medicine dispensing ring frame, and a return spring is provided between the medicine delivery barrel and the medicine dispensing ring frame; A counterweight column is placed inside the culture medium test tube; when the culture medium test tube rotates, the counterweight column stirs the culture medium of each component in the lumen of the culture medium test tube; Guide buffer sliders are fixedly installed at both ends of the counterweight column, and a stirring rod is provided on the outside of the counterweight column; The inner side of the test tube cover is fastened with an air jet, and an air jet piston is mounted inside the air jet. One end of the piston driving arm is fastened to the top of the air jet piston, and the other end is fastened to a magnetic block. A one-way air suction valve is fixed to the top of the air jet. An air jet hole is opened on the side of the piston drive arm, and the air jet hole communicates with the inner cavity of the piston drive arm and the air jet tube. When the counterweight column approaches the test tube cap, it will first contact the magnetic block, thereby pushing the piston drive arm to move. At this time, the piston drive arm pulls the air jet piston toward the one-way suction valve, and the air jet piston squeezes the air in the inner cavity of the air jet tube and discharges it through the air jet hole. The top of the test tube cover is threadedly connected to a detection cylinder, and the inside of the test tube cover is tightly connected to an air supply pipe; a detection piston is movably installed inside the detection cylinder, and a detection spring is connected between the detection piston and the detection cylinder; A one-way air supply valve is fixedly installed on the surface of the detection piston; When the culture medium test tube is in a relatively vertical state, the test tube cover is located above the end of the culture medium test tube, and the counterweight column is located at the bottom of the inner cavity of the culture medium test tube under the action of its own gravity. The magnetic attraction of the magnetic block to the counterweight column forces the piston drive arm to pull the jet piston downward. The inner cavity of the jet cylinder and the upper part of the jet piston store a large amount of air. When the culture medium test tube is stirred, the motor drives the central disk to rotate, and the airflow in the inner cavity of the jet cylinder is squeezed by the jet piston and output from the jet hole to the culture medium in the inner cavity of the culture medium test tube, thereby utilizing bubble disturbance to enhance the intensity of culture medium mixing; the test tube cover rotates to the bottom of the culture medium test tube, and the jet piston squeezes the air in the inner cavity of the jet cylinder again. If there is a leak between the culture medium test tube and the test tube cover at this time, as the jet piston presses the airflow in the inner cavity of the jet cylinder into the inner cavity of the culture medium test tube, the airflow enters the inner cavity of the culture medium test tube, causing its air pressure to increase. Combined with the fact that the test tube cover is at the bottom at this time, the culture medium will flow out directly along the leaking part, thereby alerting the operator that there is a leak in the culture medium test tube at this location; When the stirring of the culture medium test tube is completed, if the leakage location is located between the culture medium test tube and the test tube cover, the culture medium raw materials inside the culture medium test tube are discharged.

2. The automatic microbial culture medium preparation device according to claim 1, characterized in that: There are multiple drug delivery cartridges, which are arranged in a circular shape with equal angles on the surface of the drug dispensing ring frame, and each drug delivery cartridge is filled with culture medium of different components.

3. The automatic microbial culture medium preparation device according to claim 1, characterized in that: A tightening wedge block is fixedly connected to the bottom of the test tube cover, a tightening screw is threadedly connected to the test tube cover, and a tightening ring is movably installed at the bottom of the tightening screw.

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