Organic composite nano-calcium foliar fertilizer and application thereof

Abstract

The invention relates to the technical field of organic fertilizer preparation, in particular to an organic composite nano-calcium foliar fertilizer and application thereof. The organic composite nano-calcium leaf fertilizer is prepared from the following components in parts by mass: 45-55 parts of a green nano-calcium agent, 80-120 parts of an organic compound and 8-12 parts of a surfactant. The organic composite nano-calcium fertilizer is prepared by synthesizing a green nano-calcium material and reasonably matching the green nano-calcium material with organic active substances, and the organic composite nano-calcium fertilizer is sprayed on leaves in a squaring stage, a flowering and podding stage and a seed filling and mature stage of crops, so that the growth of the crops and the absorption and accumulation of calcium in edible parts are promoted, and the bioavailability and nutritional quality of calcium in agricultural products are improved; calcium-rich functional agricultural products are produced to promote calcium nutrition and health of human bodies.

Description

Technical Field

[0001] The invention relates to the technical field of organic fertilizer preparation, in particular to an organic composite nano-calcium foliar fertilizer and application thereof. Background Art

[0002] Calcium is an important element in the formation of human bones and teeth, accounting for about 99% of the total calcium in the human body. Calcium is essential for promoting bone health and maintaining normal body functions. The right amount of calcium is essential for maintaining normal physiological functions of the human body. Calcium deficiency may lead to health problems such as osteoporosis, increased risk of fractures, muscle cramps, and calcium nutrition is closely related to the prevention and treatment of cardiovascular and cerebrovascular diseases and cancer. According to the latest research, 66% of the world's population is calcium deficient, mainly due to low calcium concentration in food, low bioavailability, and insufficient intake. This phenomenon is mainly caused by a variety of factors, including unbalanced dietary structure (usually based on grains and vegetables), insufficient intake of dairy products, insufficient sunlight, and low absorption rate of calcium nutrition itself. In addition, many people lack the awareness or knowledge of calcium supplementation and fail to supplement calcium in a timely and effective manner through diet or supplements.

[0003] Calcium is not only vital to human health, but also one of the essential nutrients for plant growth and development, and is involved in the construction and maintenance of cell walls. Calcium deficiency in crops is a common problem in agricultural production, especially in acidic soils, sandy soils, or high temperature and drought conditions. Calcium deficiency may lead to necrosis of the terminal buds of crops, leaf deformity and yellowing, deterioration of fruit quality (fruit deformity, cracking of the epidermis, softening and rotting of fruits, typical examples are "bitter pit disease" in apples, "navel rot" in tomatoes and sweet peppers, and cracking and "hollow disease" in watermelons), increased sensitivity to diseases, abnormal flowering and fruiting, and poor root development. This not only directly affects the growth, yield, quality and commercial value of crops, but also indirectly affects the stability and sustainability of agricultural production. Therefore, scientific and reasonable supplementation of calcium for different crops and soil conditions can not only improve the yield and quality of crops, but also ensure food safety and promote the sustainable development of agriculture.

[0004] The low concentration and poor bioavailability of calcium in agricultural products are one of the important reasons for calcium deficiency in the human body. Since many common agricultural products contain insufficient calcium or have low bioavailability, according to the current dietary structure of residents, even if they consume a sufficient amount of these agricultural products, it is difficult to effectively absorb and utilize calcium, which ultimately leads to insufficient calcium intake in the human body. Therefore, increasing the concentration of calcium in agricultural products and the bioavailability of calcium in crops is one of the best ways to increase the effective daily intake of calcium without significantly changing the dietary habits of residents.

[0005] The application of nanotechnology in agriculture has great potential, especially in improving the absorption and utilization efficiency of plant nutrients. At present, there is a clear demand for increasing calcium content in agricultural production, but traditional fertilizer application methods often have limited effects or involve negative impacts that increase the burden on the environment. The use of nano-scale foliar fertilizers can promote the transportation and accumulation of calcium in the edible parts of crops, improve its bioavailability and the body's ability to absorb and utilize it. However, it is often difficult to achieve the desired goal by applying nano calcium fertilizers to the leaves alone. Starting from the relationship between elements and organic ligands and their coordination correlation, the foliar fertilizer formula can be adjusted to achieve the goal of increasing the calcium content in the edible part of crops, bioavailability and the quality of broad beans.

[0006] Characteristics of organic composite nano calcium fertilizer: it is organically combined with green nano calcium agent, organic agent and surfactant, and has the characteristics of convenient use, safety and high efficiency. The particle size of green nano calcium agent is between 20 and 30nm, and its particles are small, the specific surface area is high, the chemical stability is good, and the nutrition is slowly released, which improves the absorption efficiency, transportation efficiency and bioavailability of calcium in crops. Surfactant increases the effective area of ​​the contact surface, dissolves the wax structure of the leaves, and increases the solubility of the solution. Organic fertilizer not only promotes crop growth and health and the permeability and adhesion of foliar fertilizer, but also improves the biocompatibility of nano calcium fertilizer, thereby improving the slow-release efficiency of nano calcium fertilizer, and then promoting the absorption efficiency and transportation efficiency of nano calcium fertilizer. At present, there are few studies on the use of nano calcium fertilizer combined with organic agent and surfactant to improve crop leaf absorption. In the present invention, nano calcium fertilizer is combined with organic agent and surfactant to develop calcium efficient foliar fertilizer, which is expected to increase the calcium enrichment in the edible part of crops by spraying organic composite nano calcium fertilizer. Summary of the invention

[0007] The purpose of the present invention is to provide an organic composite nano-calcium foliar fertilizer and its application. The present invention provides a method for using an organic composite nano-calcium fertilizer to improve the calcium enrichment and bioavailability of agricultural products and strengthen calcium nutrition and health of the human body. The organic composite nano-calcium fertilizer is prepared by synthesizing green nano-calcium materials and rationally combining them with organic active substances. The organic composite nano-calcium fertilizer is sprayed on the leaves during the budding period, flowering and pod-setting period, and grain maturity period of the crop to promote crop growth, absorption and accumulation of calcium in the edible part, improve the bioavailability and nutritional quality of calcium in agricultural products, and produce calcium-rich functional agricultural products to promote calcium nutrition and health of the human body.

[0008] The method of the invention is simple to operate and environmentally friendly, and can not only reduce the input of traditional calcium fertilizers, but also increase crop yields and the value of calcium nutrition.

[0009] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0010] The present invention provides an organic composite nano-calcium foliar fertilizer, which is prepared from the following components by mass: 45 to 55 parts of green nano-calcium agent, 80 to 120 parts of organic compound and 8 to 12 parts of surfactant;

[0011] Preferably, the preparation method of the green nano calcium agent comprises the following steps:

[0012] (1) drying the plant stems, extracting with water, filtering, centrifuging, taking the supernatant, and filtering to obtain the plant extract;

[0013] (2) The plant extract is mixed with a calcium chloride solution, the pH value is adjusted and then reacted, the precipitate is collected by centrifugation, and the precipitate is dried and then calcined to obtain a green nano calcium agent.

[0014] Preferably, the plant stems in step (1) are broad bean stems and leaves and / or tea stems.

[0015] Preferably, the mass ratio of the plant stems after drying in step (1) to water is 1:8-12; the water extraction temperature is 70-80°C, the time is 55-65 min, and the rotation speed is 200-240 rpm.

[0016] Preferably, in step (2), the volume ratio of the plant extract to the calcium chloride solution is 5:80-120; the concentration of the calcium chloride solution is 0.04-0.06 M; the pH adjuster is a sodium hydroxide solution with a concentration of 0.8-1.2 M; the pH is adjusted to 11.5-12.5; and the reaction time is 1.5-2.5 h.

[0017] Preferably, the calcination temperature in step (2) is 550-650° C. and the calcination time is 2-3 h.

[0018] Preferably, the organic compound is sorbitol.

[0019] Preferably, the surfactant is Tween-20, alkylphenol polyoxyethylene ether or fatty alcohol polyoxyethylene ether.

[0020] The invention also provides application of the organic composite nano-calcium foliar fertilizer in broad bean planting.

[0021] Preferably, the organic composite nano-calcium foliar fertilizer is diluted and sprayed on the leaves during the budding stage, flowering and podding stage, and grain maturity stage of broad beans.

[0022] Preferably, the dilution multiple is 45 to 65 times; 35 to 45 L / mu is sprayed each time.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The organic fertilizer provided by the present invention can improve the absorption efficiency of calcium, because the tiny particle size of the green synthetic nano calcium agent makes it easier to be absorbed through the leaf surface, and has the effect of slow-release calcium when entering the plant body, and can slowly release and provide calcium fertilizer. The organic compound sugar alcohol can enhance the biocompatibility and absorbability of calcium in the broad bean body, thereby improving the bioavailability of calcium. Nano calcium combines with organic complex calcium to help the transportation and transformation of calcium in the plant body, ensuring that it can effectively reach the required parts, such as new leaves and fruits. This composite calcium fertilizer can enhance the resistance of plants to adversity, such as pests and diseases, and promote the overall health of plants. Nano calcium combined with organic compounds can also help to improve the nutritional content of broad beans. By improving the effectiveness of calcium and plant growth, foliar spraying can potentially increase the final yield of broad beans and the calcium content of broad beans.

[0025] The reasons why the present invention adopts broad bean stalks as plant-derived raw materials are as follows: 1) the content of biologically active ingredients is high, especially rich in active substances such as polyphenols, amino acids and natural sugars, and these ingredients can effectively promote the reduction and stabilization of nano calcium; 2) broad beans have strong biocompatibility, which helps to improve the bioavailability of calcium; 3) compared with other plant materials, broad bean stalks and leaves are more convenient and low-cost to obtain, and are usually waste in agriculture, which can achieve resource recycling and improve agricultural economic benefits; 4) the plant stock solution of broad bean stalks and leaves is used as a synthetic raw material to improve the safety of the product and make the synthesis method more environmentally friendly.

[0026] The sorbitol added in the present invention is a sugar alcohol chelate of calcium. Sugar alcohol calcium is mainly a sugar alcohol ion chelate product made by chelating sugar alcohol and calcium ions together using the chelation technology. The main purpose of adding is to promote the absorption and utilization of calcium fertilizer by crops, improve the utilization rate, and supplement calcium more efficiently; sugar alcohol itself has a good moisturizing function and can stay on the leaves for a long time, so it prolongs the time for the leaves to absorb calcium elements, which also improves the absorption and utilization of calcium by crops.

[0027] The foliar fertilizer provided by the present invention can significantly increase crop yield, calcium content in edible agricultural products and bioavailability, and has a reinforcing effect on human calcium nutrition and health. The present invention adopts a composite method of organic ingredients and nanotechnology, is simple to operate, reduces the use of chemical fertilizers, is environmentally friendly and safe, and is suitable for the sustainable development needs of modern agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0029] Figure 1 Statistics of calcium content in the edible part (grains) of broad beans in different experimental groups.

[0030] Figure 2 Statistics of bioavailable content of broad bean seeds in different experimental groups.

[0031] Figure 3 Statistics of phytic acid / calcium ratios in broad bean seeds of different experimental groups.

[0032] Figure 4 Statistics of theoretical daily calcium intake from broad bean seeds in different experimental groups. DETAILED DESCRIPTION

[0033] The invention provides an organic composite nano-calcium foliar fertilizer, which is prepared from the following components in parts by weight: 45 to 55 parts of green nano-calcium agent, 80 to 120 parts of organic compounds and 8 to 12 parts of surfactants; preferably 47 to 53 parts of green nano-calcium agent, 90 to 110 parts of organic compounds and 9 to 11 parts of surfactants; further preferably 49 to 51 parts of green nano-calcium agent, 100 parts of organic compounds and 10 parts of surfactants; more preferably 50 parts of green nano-calcium agent, 100 parts of organic compounds and 10 parts of surfactants.

[0034] In the present invention, the preparation method of the green nano calcium agent comprises the following steps:

[0035] (1) drying the plant stems, extracting with water, filtering, centrifuging, taking the supernatant, and filtering to obtain the plant extract;

[0036] (2) The plant extract is mixed with a calcium chloride solution, the pH value is adjusted and then reacted, the precipitate is collected by centrifugation, and the precipitate is dried and then calcined to obtain a green nano calcium agent.

[0037] In the present invention, the plant stems in step (1) are broad bean stems and leaves and / or tea stems; preferably broad bean stems and leaves (all parts of the above-ground broad bean plant except the parts with direct economic value, including stems and leaves).

[0038] In the present invention, the mass ratio of the plant stems after drying in step (1) to water is 1:8-12; preferably 1:9-11; and more preferably 1:10.

[0039] In the present invention, the water extraction temperature in step (1) is 70-80°C, the time is 55-65min, and the rotation speed is 200-240rpm; preferably, the water extraction temperature is 72-78°C, the time is 57-63min, and the rotation speed is 210-230rpm; more preferably, the water extraction temperature is 74-76°C, the time is 59-61min, and the rotation speed is 220rpm; more preferably, the water extraction temperature is 75°C, the time is 60min, and the rotation speed is 220rpm.

[0040] In the present invention, the volume ratio of the plant extract to the calcium chloride solution in step (2) is 5:80-120; preferably 5:90-110; and more preferably 5:100.

[0041] In the present invention, the concentration of the calcium chloride solution in step (2) is 0.04-0.06M, preferably 0.05M.

[0042] In the present invention, the pH adjuster in step (2) is a sodium hydroxide solution with a concentration of 0.8 to 1.2 M; preferably 0.9 to 1.1 M; more preferably 1 M.

[0043] In the present invention, the pH value in step (2) is adjusted to 11.5-12.5; preferably 11.7-12.3; more preferably 11.9-12.1; and more preferably 12.

[0044] In the present invention, the reaction time of step (2) is 1.5 to 2.5 hours, preferably 2 hours.

[0045] In the present invention, the calcination temperature in step (2) is 550-650°C and the time is 2-3h; preferably, the calcination temperature is 570-630°C and the time is 2.5h; further preferably, the calcination temperature is 590-610°C and the time is 2.5h; more preferably, the calcination temperature is 600°C and the time is 2.5h.

[0046] In the present invention, the organic compound is sorbitol.

[0047] In the present invention, the surfactant is Tween-20, alkylphenol polyoxyethylene ether or fatty alcohol polyoxyethylene ether; preferably Tween-20.

[0048] The invention also provides application of the organic composite nano-calcium foliar fertilizer in broad bean planting.

[0049] In the present invention, the organic composite nano-calcium foliar fertilizer is diluted and sprayed on the leaves of broad beans during the budding stage, flowering and podding stage, and grain maturity stage.

[0050] In the present invention, the dilution multiple is 45 to 65 times; preferably 47 to 63 times; more preferably 49 to 61 times; and more preferably 50 times.

[0051] In the present invention, each spraying is 35-45 L / mu; preferably 37-43 L / mu; more preferably 39-41 L / mu; more preferably 40 L / mu.

[0052] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0053] Example 1

[0054] A method for preparing an organic composite nano-calcium foliar fertilizer comprises the following steps:

[0055] 1. Select broad bean stems and leaves as plant materials, wash them and dry them naturally at 25℃ for 72h.

[0056] 2. Grind the dried plant material into powder using a crusher, put it into a sample bag and store it in a refrigerator at 4°C for later use. Extract according to the ratio of 1:8 (plant material: deionized water): put the plant material into a round-bottom flask, add deionized water, add a stirrer and cover the lid. Heat to 70°C in a magnetic stirring constant temperature water bath, stir at 200 rpm for 55 minutes, and cool to room temperature.

[0057] 3. The mixture was filtered through a vacuum filtration system, and the filtrate was collected and stored in a 4°C refrigerator for later use. The extract was centrifuged at 6000 rpm for 15 minutes, and the supernatant after centrifugation was collected and filtered through a 0.22 μm water filter membrane. The original plant extract was stored in a 4°C refrigerator.

[0058] 4. Place a beaker on a magnetic stirrer, add 90 mL of 0.05 M calcium chloride solution, and start stirring. Slowly add 5 mL of the plant extract solution to the calcium chloride solution, and slowly adjust the pH to 11.5 with 1 M sodium hydroxide solution. Continue stirring the reaction for 2 hours until the solution becomes white and turbid, indicating that the precursor of nano calcium particles is formed.

[0059] Reaction formula: CaCl2+2NaOH→Ca(OH)2+2NaCl

[0060] After the reaction is completed, the mixed solution is centrifuged to separate the precipitated nano calcium particle precursor. After washing, the precipitate is dried in a vacuum dryer for 24 hours to obtain a white powder. The white powder is placed in a muffle furnace and calcined at 550°C for 2 hours to generate a green nano calcium agent (Green Nano Calcium Agent) precursor. The calcined calcium oxide particles are ground with a mortar or a high-throughput grinder to obtain a uniform nanoscale green nano calcium agent (Green Nano Calcium Agent) powder.

[0061] 5. Using water as solvent, mix the sorbitol solution and the calcium source in a molar ratio of 2:1, use 0.05M sodium hydroxide solution and adjust the pH to 8, stir the mixture in a water bath at 40°C for 2 hours to generate a sorbitol complex, and finally concentrate to obtain a sorbitol stock solution for standby use.

[0062] The chelation reaction equation is C6H 14 O6+CaCl2→(C6H 13 O6)2Ca+2HCl

[0063] 6. Mix the nano-scale green nano-calcium powder, sorbitol stock solution and fatty alcohol polyoxyethylene ether in a mass ratio of 45:80:8 to obtain an organic composite nano-calcium foliar fertilizer.

[0064] Example 2

[0065] A method for preparing an organic composite nano-calcium foliar fertilizer comprises the following steps:

[0066] 1. Select broad bean stems and leaves and tea stems of equal quality as plant materials, wash them and dry them naturally at 25℃ for 96 hours.

[0067] 2. Grind the dried plant material into powder using a crusher, put it into a sample bag and store it in a refrigerator at 4°C for later use. Extract according to the ratio of 1:12 (plant material: deionized water): put the plant material into a round-bottom flask, add deionized water, add a stirrer and cover it with a lid. Heat to 77°C in a magnetic stirring constant temperature water bath, stir at 240 rpm for 65 minutes, and cool to room temperature.

[0068] 3. The mixture was filtered through a vacuum filtration system, and the filtrate was collected and stored in a 4°C refrigerator for later use. The extract was centrifuged at 6000 rpm for 15 minutes, and the supernatant after centrifugation was collected and filtered through a 0.22 μm water filter membrane. The original plant extract was stored in a 4°C refrigerator.

[0069] 4. Place a beaker on a magnetic stirrer, add 120 mL of 0.05 M calcium chloride solution, and start stirring. Slowly add 5 mL of the plant extract solution to the calcium chloride solution, and slowly adjust the pH to 12.5 with 1 M sodium hydroxide solution. Continue stirring the reaction for 2 hours until the solution becomes white and turbid, indicating that the precursor of nano calcium particles is formed.

[0070] Reaction formula: CaCl2+2NaOH→Ca(OH)2+2NaCl

[0071] After the reaction is completed, the mixed solution is centrifuged to separate the precipitated nano calcium particle precursor. After washing, the precipitate is dried in a vacuum dryer for 24 hours to obtain a white powder. The white powder is placed in a muffle furnace and calcined at 650°C for 3.5 hours to generate a green nano calcium agent precursor. The calcined calcium oxide particles are ground in a mortar to obtain a uniform nano-scale green nano calcium agent powder.

[0072] 5. Using water as solvent, mix the sorbitol solution and the calcium source in a molar ratio of 2:1, use 0.05M sodium hydroxide solution and adjust the pH to 9, stir the mixture in a water bath at 60°C for 2 hours to generate a sorbitol complex, and finally concentrate to obtain a sorbitol stock solution for standby use.

[0073] The chelation reaction equation is C6H 14 O6+CaCl2→(C6H 13 O6)2Ca+2HCl

[0074] 6. Mix the nano-scale green nano-calcium powder, sorbitol stock solution and alkylphenol polyoxyethylene ether in a mass ratio of 55:120:12 to obtain an organic composite nano-calcium foliar fertilizer.

[0075] Example 3

[0076] A method for preparing an organic composite nano-calcium foliar fertilizer comprises the following steps:

[0077] 1. Select broad bean stems and leaves as plant materials, wash them and dry them naturally at 25℃ for 80 hours.

[0078] 2. Grind the dried plant material into powder using a crusher, put it into a sample bag and store it in a refrigerator at 4°C for later use. Extract according to the ratio of 1:10 (plant material: deionized water): put the plant material into a round-bottom flask, add deionized water, add a stirrer and cover the lid. Heat to 75°C in a magnetic stirring constant temperature water bath, stir at 220 rpm for 60 minutes, and cool to room temperature.

[0079] 3. The mixture was filtered through a vacuum filtration system, and the filtrate was collected and stored in a 4°C refrigerator for later use. The extract was centrifuged at 6000 rpm for 15 minutes, and the supernatant after centrifugation was collected and filtered through a 0.22 μm water filter membrane. The original plant extract was stored in a 4°C refrigerator.

[0080] 4. Place a beaker on a magnetic stirrer, add 100 mL of 0.05 M calcium chloride solution, and start stirring. Slowly add 5 mL of the plant extract solution to the calcium chloride solution, and slowly adjust the pH to 12 with 1 M sodium hydroxide solution. Continue stirring the reaction for 2 hours until the solution becomes white and turbid, indicating that the precursor of nano calcium particles is formed.

[0081] Reaction formula: CaCl2+2NaOH→Ca(OH)2+2NaCl

[0082] After the reaction is completed, the mixed solution is centrifuged to separate the precipitated nano calcium particle precursor. After washing, the precipitate is dried in a vacuum dryer for 24 hours to obtain a white powder. The white powder is placed in a muffle furnace and calcined at 600°C for 2.5 hours to generate a green nano calcium agent precursor. The calcined calcium oxide particles are ground with a high-throughput grinder to obtain a uniform nanoscale green nano calcium agent powder.

[0083] 5. Using water as solvent, mix the sorbitol solution and the calcium source in a molar ratio of 2:1, use 0.05M sodium hydroxide solution and adjust the pH to 8.5, stir the mixture in a water bath at 50°C for 2 hours to generate a sorbitol complex, and finally concentrate to obtain a sorbitol stock solution for standby use.

[0084] The chelation reaction equation is C6H 14 O6+CaCl2→(C6H 13 O6)2Ca+2HCl

[0085] 6. Mix the nano-scale green nano-calcium powder, sorbitol stock solution and Tween-20 in a mass ratio of 50:100:10 to obtain an organic composite nano-calcium foliar fertilizer.

[0086] Example 4

[0087] An application method of an organic composite nano-calcium foliar fertilizer: dilute the organic composite nano-calcium foliar fertilizer obtained by the method of Example 1 by 45 times and spray it on the leaves of broad beans during the budding stage, flowering and podding stage, and grain maturity stage, with 35L / mu sprayed each time.

[0088] Example 5

[0089] An application method of an organic composite nano-calcium foliar fertilizer: dilute the organic composite nano-calcium foliar fertilizer obtained by the method of Example 2 by 65 times and spray it on the leaves of broad beans during the budding stage, flowering and podding stage, and grain maturity stage, with 45L / mu sprayed each time.

[0090] Example 6

[0091] An application method of an organic composite nano-calcium foliar fertilizer: dilute the organic composite nano-calcium foliar fertilizer obtained by the method of Example 3 by 62.5 times and spray it on the leaves of broad beans during the budding stage, flowering and podding stage, and grain maturity stage, with 40L / mu sprayed each time.

[0092] Experimental Example 1

[0093] Choose a plot of land to plant broad beans, and choose a broad bean variety with white flowers and large grains.

[0094] Among them, the application method of Example 6 was used as the experimental group (organic composite nano calcium fertilizer);

[0095] Control group: sprayed with water only.

[0096] Except for the different fertilizer application, the other growth management methods of each experimental group were exactly the same, and two groups were set up for each group. After the broad beans were harvested at maturity, the 100-grain weight and yield of broad beans were investigated, and the protein and phytic acid content of broad bean seeds were determined. The results were averaged, as shown in Tables 1 and 2.

[0097] Table 1 Effect of spraying organic composite nano-calcium fertilizer on the yield of broad bean with white flowers and large grains

[0098]

[0099] Table 2 Effect of spraying organic composite nano-calcium fertilizer on broad bean quality

[0100]

[0101] The results showed that compared with the control treatment of spraying water, spraying organic composite nano-calcium fertilizer can increase the 100-grain weight of broad bean grains and increase broad bean yield (Table 1); compared with the control treatment, spraying organic composite nano-calcium fertilizer can slightly increase the protein content of broad bean grains and reduce the phytic acid content (Table 2).

[0102] Experimental Example 2

[0103] The calcium content in the edible part of broad bean of each test group in Experimental Example 1 was determined by inductively coupled plasma optical emission spectrometry (ICP-OES), and the bioavailability of calcium in the edible part of broad bean was calculated. The results are shown in Table 3.

[0104] Table 3 Calcium content in edible part

[0105]

[0106] The calcium content of the edible part of broad beans in the experimental group sprayed with organic composite nano-calcium fertilizer was as high as 187.95 mg / 100g, which was significantly increased by 43.6% compared with the control group with a calcium content of 130.88 mg / 100g. Figure 1 ), * represents p<0.05, ** represents p<0.01.

[0107] The bioavailability of calcium in the edible part of broad beans in the experimental group sprayed with organic composite nano-calcium fertilizer was as high as 17.52%, which was significantly increased by 30.44% compared with the bioavailability of calcium in the edible part of the control group (13.43%). Figure 2 ).

[0108] Experimental Example 3

[0109] The ratio of phytic acid to calcium in broad bean seeds of each test group in Experimental Example 1 was counted and calculated. The results are as follows: Figure 3 As shown: the ratio of phytic acid to calcium in broad bean seeds sprayed with organic composite nano-calcium fertilizer was as low as 0.22, which was 34.3% lower than the ratio of phytic acid to calcium in the control seeds of 0.33.

[0110] Experimental Example 4

[0111] The theoretical daily calcium intake (EDI) of broad bean seeds of each test group in Experimental Example 1 was counted and calculated, wherein the calcium intake was assumed to be 30 g broad bean seeds per day for an adult, and the calculation formula was: EDI (mg / day) = calcium content (mg / kg) × 30 / 1000.

[0112] The results are as follows Figure 4 As shown: the theoretical daily calcium intake (EDI) of broad bean seeds in the experimental group sprayed with organic composite nano-calcium fertilizer was as high as 56.38 mg / day, which was 43.6% higher than the theoretical daily calcium intake of 39.26 mg / day of calcium broad bean seeds in the control group.

[0113] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An organic composite nano-calcium foliar fertilizer, characterized in that: The method is prepared from the following components by weight: 45 to 55 parts of green nano calcium agent, 80 to 120 parts of organic compound and 8 to 12 parts of surfactant; The preparation method of the green nano calcium agent comprises the following steps: (1) drying the plant stems, extracting with water, filtering, centrifuging, taking the supernatant, and filtering to obtain the plant extract; (2) The plant extract is mixed with a calcium chloride solution, the pH value is adjusted and then reacted, the precipitate is collected by centrifugation, and the precipitate is dried and then calcined to obtain a green nano calcium agent.

2. An organic composite nano-calcium foliar fertilizer according to claim 1, characterized in that: The plant stems in step (1) are broad bean stems and leaves and / or tea stems.

3. An organic composite nano-calcium foliar fertilizer according to claim 1 or 2, characterized in that: The mass ratio of the plant stems after drying in step (1) to water is 1:8-12; the water extraction temperature is 70-80°C, the time is 55-65 minutes, and the rotation speed is 200-240rpm.

4. An organic composite nano-calcium foliar fertilizer according to claim 1, characterized in that: In step (2), the volume ratio of the plant extract to the calcium chloride solution is 5:80-120; the concentration of the calcium chloride solution is 0.04-0.06M; the pH adjuster is a sodium hydroxide solution with a concentration of 0.8-1.2M; the pH is adjusted to 11.5-12.5; and the reaction time is 1.5-2.5h.

5. The organic composite nano-calcium foliar fertilizer according to claim 1, characterized in that: The calcination temperature in step (2) is 550-650° C. and the calcination time is 2-3 hours.

6. The organic composite nano-calcium foliar fertilizer according to claim 1, characterized in that: The organic compound is sorbitol.

7. The organic composite nano-calcium foliar fertilizer according to claim 1, characterized in that: The surfactant is Tween-20, alkylphenol polyoxyethylene ether or fatty alcohol polyoxyethylene ether.

8. Use of the organic composite nano-calcium foliar fertilizer according to any one of claims 1 to 7 in broad bean planting.

9. The use according to claim 8, characterized in that: The organic composite nano-calcium foliar fertilizer is diluted and sprayed on the leaves of broad beans during the budding stage, flowering and pod-setting stage, and grain-filling and ripening stage.

10. The use according to claim 9, characterized in that: The dilution multiple is 45 to 65 times; 35 to 45 L / mu is sprayed each time.

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