Long-acting regenerated collagen and preparation method thereof

Abstract

The invention discloses long-acting regenerated collagen and a preparation method thereof, the long-acting regenerated collagen comprises pig collagen and hollow poly-L-lactic acid microspheres, and the ratio of the pig collagen to the hollow poly-L-lactic acid microspheres is 7: 3. According to the invention, the double-effect long-acting collagen and the type I pig collagen have the effect of space-occupying filling, the hollow poly-L-lactic acid of 50-80 microns is added, the poly-L-lactic acid has the effects of stimulating fibroblasts and self-collagen growth and the effect of double-effect regeneration, the collagen is firstly filled and supplemented in a space-occupying manner, and then the self-synthesis and growth of the collagen are stimulated, so that the collagen can be regenerated in a space-occupying manner. And meanwhile, the collagen is free from residues, safe and effective, and stimulated collagen is supplemented.

Description

Technical Field

[0001] The present invention relates to the technical field of regenerated collagen, in particular to a long-acting regenerated collagen and a preparation method thereof. Background Art

[0002] Collagen and elastin in the skin are two extremely important structural proteins. Together, they constitute the extracellular matrix of the skin, providing the skin with the necessary structural support and functional properties. Collagen is one of the most abundant proteins in the human body, accounting for about 30% of the total body protein. In the skin, collagen is particularly abundant, mainly in the dermis, with a content of up to 70% to 80%. It is composed of three polypeptide chains wound in a spiral form to form a triple helix structure, which gives collagen extremely high stability and toughness. The main function of collagen is to provide structural support for the skin and maintain the firmness and elasticity of the skin. With age, the synthesis of collagen will gradually decrease, and at the same time, due to the action of matrix metalloproteinases, collagen will gradually be degraded. These factors together lead to the formation of wrinkles, decreased elasticity and sagging skin during skin aging.

[0003] The existing publication number CN118388666A discloses a polypeptide for promoting the regeneration of skin collagen and elastin and a preparation method. The present invention innovatively designs a fusion protein, which plays an important role in promoting fibroblast proliferation, inhibiting metalloproteinase activity, and enhancing collagen and elastin expression by combining elastin polypeptide with collagen polypeptide and using an amino acid sequence designed by using an elastin fragment rich in VAPGXG motif and a core fragment of type I collagen α2 chain; the present invention utilizes genetic engineering technology to achieve efficient expression and purification of the fusion protein in an Escherichia coli expression system, significantly improving production efficiency and reducing costs with strong scalability; the transdermal peptide TAT sequence added to the N-terminus of the fusion protein produced by the present invention enhances its penetration ability in the skin, thereby improving the bioavailability and efficacy of the present invention in the deep layer of the skin.

[0004] The collagen in the prior art needs to be continuously injected for filling and is absorbed quickly, and cannot self-stimulate and promote regeneration. Therefore, we propose a long-acting regenerative collagen and a preparation method thereof. Summary of the invention

[0005] The purpose of the present invention is to provide a long-acting regenerated collagen and a preparation method thereof to solve the problems in the prior art.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a long-acting regenerated collagen, comprising porcine collagen and hollow poly-L-lactic acid microspheres, wherein the ratio of the porcine collagen to the hollow poly-L-lactic acid microspheres is 7:3.

[0007] Preferably, the hollow poly-L-lactic acid microspheres are hollow poly-L-lactic acid microspheres of 50-80 microns.

[0008] A method for preparing a long-acting regenerated collagen, using the long-acting regenerated collagen, comprising the following steps:

[0009] S1. Preparation of porcine collagen solution: extracting porcine collagen by electrolytic centrifugal purification process, dissolving porcine collagen in a solvent to form a porcine collagen solution;

[0010] S2. Preparation of hollow poly-L-lactic acid microspheres: hollow poly-L-lactic acid microspheres of 50-80 μm are used;

[0011] S3, mixing: adding the hollow poly-L-lactic acid microspheres into the porcine collagen solution and mixing;

[0012] S4, centrifugation: centrifuging the mixture by a centrifuge to make the poly-L-lactic acid microspheres evenly distributed in the porcine collagen solution;

[0013] S5. Cross-linking: connect collagen molecules to each other to form a stable collagen network structure.

[0014] Preferably, the pH value and ionic strength need to be adjusted in S1 to make the porcine collagen in a soluble state.

[0015] Preferably, the mixing in S3 is achieved by using a stirring device or an ultrasonic device to mix the hollow poly-L-lactic acid microspheres and the porcine collagen solution.

[0016] Preferably, the method further includes S6, coagulation: allowing the long-acting regenerated collagen to coagulate to form a three-dimensional network structure.

[0017] Compared with the prior art, the beneficial effects of the present invention are: dual-effect long-acting collagen, type I porcine collagen itself has a place-filling effect, and 50-80 micron hollow poly-L-lactic acid is added at the same time. At the same time, poly-L-lactic acid has the function of stimulating fibroblasts and self-collagen growth, and has a dual-effect regeneration effect, first occupying the place to fill and supplement collagen, and then stimulating the self-synthesis and growth of collagen, while achieving no residue, safe and effective, and supplementing and stimulating collagen. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0019] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0020] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention.

[0021] See also Figure 1 In an embodiment of the present invention, a long-acting regenerated collagen comprises porcine collagen and hollow poly-L-lactic acid microspheres, wherein the ratio of the porcine collagen to the hollow poly-L-lactic acid microspheres is 7:3.

[0022] Preferably, the hollow poly-L-lactic acid microspheres are hollow poly-L-lactic acid microspheres of 50-80 microns.

[0023] Long-lasting regenerative collagen: composed of 70% type 1 porcine collagen and 30% hollow poly-L-lactic acid microspheres of 50 to 80 microns.

[0024] A method for preparing a long-acting regenerated collagen, using the long-acting regenerated collagen, comprising the following steps:

[0025] S1. Preparation of porcine collagen solution: extracting porcine collagen by electrolytic centrifugal purification process, dissolving porcine collagen in a solvent to form a porcine collagen solution;

[0026] S2. Preparation of hollow poly-L-lactic acid microspheres: hollow poly-L-lactic acid microspheres of 50-80 μm are used;

[0027] S3, mixing: adding the hollow poly-L-lactic acid microspheres into the porcine collagen solution and mixing;

[0028] S4, centrifugation: centrifuging the mixture by a centrifuge to make the poly-L-lactic acid microspheres evenly distributed in the porcine collagen solution;

[0029] S5. Cross-linking: connect collagen molecules to each other to form a stable collagen network structure.

[0030] It also includes S6 and coagulation: allowing the long-lasting regenerated collagen to coagulate and form a three-dimensional network structure.

[0031] Preferably, the pH value and ionic strength need to be adjusted in S1 to make the porcine collagen in a soluble state.

[0032] Preferably, the mixing in S3 is achieved by using a stirring device or an ultrasonic device to mix the hollow poly-L-lactic acid microspheres and the porcine collagen solution.

[0033] Porcine collagen is evenly distributed and exists in the form of a collagen network structure scaffold. Through centrifugation and electronic distribution technology, the poly-L-lactic acid solid microspheres are evenly distributed with the collagen network structure to avoid accumulation and excessive stimulation of regeneration in some areas.

[0034] Double-effect long-lasting collagen, type I porcine collagen itself has a space-filling effect, and 50-80 micron hollow poly-L-lactic acid is added at the same time. At the same time, poly-L-lactic acid has the function of stimulating fibroblasts and self-collagen growth, and has a double-effect regeneration effect, first filling the space to supplement collagen, and then stimulating the self-synthesis and growth of collagen, while achieving no residue, safe and effective, supplementing and stimulating collagen.

[0035] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A long-lasting regenerated collagen, characterized in that: The invention comprises porcine collagen and hollow poly-L-lactic acid microspheres, wherein the ratio of the porcine collagen to the hollow poly-L-lactic acid microspheres is 7:

3.

2. The long-acting regenerated collagen according to claim 1, characterized in that: The hollow poly-L-lactic acid microspheres are hollow poly-L-lactic acid microspheres of 50-80 microns.

3. A method for preparing long-lasting regenerated collagen, characterized in that: Using a long-acting regenerated collagen according to any one of claims 1-2; comprising the following steps: S1. Preparation of porcine collagen solution: extracting porcine collagen by electrolytic centrifugal purification process, dissolving porcine collagen in a solvent to form a porcine collagen solution; S2. Preparation of hollow poly-L-lactic acid microspheres: hollow poly-L-lactic acid microspheres of 50-80 μm are used; S3, mixing: adding the hollow poly-L-lactic acid microspheres into the porcine collagen solution and mixing; S4, centrifugation: centrifuging the mixture by a centrifuge to make the poly-L-lactic acid microspheres evenly distributed in the porcine collagen solution; S5. Cross-linking: connect collagen molecules to each other to form a stable collagen network structure.

4. The method for preparing long-acting regenerated collagen according to claim 3, characterized in that: In the S1, the pH value and ionic strength need to be adjusted to make the porcine collagen in a soluble state.

5. The method for preparing long-acting regenerated collagen according to claim 3, characterized in that: The mixing in S3 is achieved by using a stirring device or an ultrasonic device to mix the hollow poly-L-lactic acid microspheres and the porcine collagen solution.

6. The method for preparing long-acting regenerated collagen according to claim 1, characterized in that: It also includes S6 and coagulation: allowing the long-lasting regenerated collagen to coagulate and form a three-dimensional network structure.

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